keyword
MENU ▼
Read by QxMD icon Read
search

Ins 1e

keyword
https://www.readbyqxmd.com/read/29078993/distinguishing-signal-from-autofluorescence-in-cryogenic-correlated-light-and-electron-microscopy-of-mammalian-cells
#1
Stephen D Carter, Shrawan K Mageswaran, Zachary J Farino, João I Mamede, Catherine M Oikonomou, Thomas J Hope, Zachary Freyberg, Grant J Jensen
In cryogenic correlated light and electron microscopy (cryo-CLEM), frozen targets of interest are identified and located on EM grids by fluorescence microscopy and then imaged at higher resolution by cryo-EM. Whilst working with these methods, we discovered that a variety of mammalian cells exhibit strong punctate autofluorescence when imaged under cryogenic conditions (80 K). Autofluorescence originated from multilamellar bodies (MLBs) and secretory granules. Here we describe a method to distinguish fluorescent protein tags from these autofluorescent sources based on the narrower emission spectrum of the former...
October 25, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/29051320/combinatorial-treatment-with-mtor-inhibitors-and-streptozotocin-leads-to-synergistic-in-vitro-and-in-vivo-antitumor-effects-in-insulinoma-cells
#2
Julien Bollard, Céline Patte, Patrick Massoma, Isabelle Goddard, Nicolas Gadot, Noura Benslama, Valérie Hervieu, Carole Ferraro-Peyret, Martine Cordier-Bussat, Jean-Yves Scoazec, Colette Roche, Thomas Walter, Cécile Vercherat
Streptozotocin (STZ)-based chemotherapy is the first-line chemotherapy recommended for advanced pancreatic neuroendocrine tumors (pNETs), while targeted therapies, including mTOR inhibitors, are available in second-line treatment. Unfortunately objective response rates to both treatments are limited. Since mTOR pathway activation, commonly observed in pNETs, has been reported as one of the major mechanisms accounting for chemoresistance, we investigated the potential benefit of mTOR inhibition combined with STZ treatment in a subset of pNETs, namely insulinomas...
October 19, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29046394/recombinant-human-islet-amyloid-polypeptide-forms-shorter-fibrils-and-mediates-%C3%AE-cell-apoptosis-via-generation-of-oxidative-stress
#3
Richa Dubey, Pooja Minj, Nikita Malik, Devika M Sardesai, Shruti H Kulkarni, Jhankar D Acharya, Neel S Bhavesh, Shilpy Sharma, Ashutosh Kumar
Protein aggregation play an important role in many human diseases including Alzheimer's, Parkinson's and Type 2 diabetes mellitus (T2DM). Human islet amyloid polypeptide (hIAPP) forms amyloid plaques in pancreas of T2DM subjects that are involved in deteriorating islet function and in mediating β- cell apoptosis. However, the detailed mechanism of action, structure and nature of toxic hIAPP species responsible for this effect remains elusive till date mainly due to the high cost associated with the chemical synthesis of pure peptide required for these studies...
October 18, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/29044173/palmitate-induced-lipotoxicity-alters-acetylation-of-multiple-proteins-in-clonal-%C3%AE-cells-and-human-pancreatic-islets
#4
Federica Ciregia, Marco Bugliani, Maurizio Ronci, Laura Giusti, Claudia Boldrini, Maria R Mazzoni, Sandra Mossuto, Francesca Grano, Miriam Cnop, Lorella Marselli, Gino Giannaccini, Andrea Urbani, Antonio Lucacchini, Piero Marchetti
Type 2 diabetes is characterized by progressive β cell dysfunction, with lipotoxicity playing a possible pathogenetic role. Palmitate is often used to examine the direct effects of lipotoxicity and it may cause mitochondrial alterations by activating protein acetylation. However, it is unknown whether palmitate influences protein acetylation in β cells. We investigated lysine acetylation in mitochondrial proteins from INS-1E β cells (INS-1E) and in proteins from human pancreatic islets (HPI) after 24 h palmitate exposure...
October 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28993191/the-anti-aging-protein-klotho-is-induced-by-gaba-therapy-and-exerts-protective-and-stimulatory-effects-on-pancreatic-beta-cells
#5
Gérald J Prud'homme, Yelena Glinka, Merve Kurt, Wenjuan Liu, Qinghua Wang
Systemic gamma-aminobutyric acid (GABA) therapy prevents or ameliorates type 1 diabetes (T1D), by suppressing autoimmune responses and stimulating pancreatic beta cells. In beta cells, it increases insulin secretion, prevents apoptosis, and induces regeneration. It is unclear how GABA mediates these effects. We hypothesized that Klotho is involved. It is a multi-functional protein expressed in the kidneys, brain, pancreatic beta cells, other tissues, and is cell-bound or soluble. Klotho knockout mice display accelerated aging, and in humans Klotho circulating levels decline with age, renal disease and diabetes...
December 2, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28990659/persistence-of-stat-1-inhibition-and-induction-of-cytokine-resistance-in-pancreatic-%C3%AE-cells-treated-with-st-john-s-wort-and-its-component-hyperforin
#6
Michela Novelli, Pascale Beffy, Alex Gregorelli, Svetlana Porozov, Fabrizio Mascia, Chiara Vantaggiato, Pellegrino Masiello, Marta Menegazzi
OBJECTIVES: St John's wort extract (SJW) and its component hyperforin (HPF) were shown to potently inhibit cytokine-induced STAT-1 and NF-κB activation in pancreatic β cells and protect them against injury. This study aimed at exploring the time course of STAT-1 inhibition afforded by these natural compounds in the β-cell line INS-1E. METHODS: INS-1E cells were pre-incubated with SJW extract (2-5 μg/ml) or HPF (0.5-2 μm) and then exposed to a cytokine mixture...
October 9, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28983714/a-novel-phenylchromane-derivative-increases-the-rate-of-glucose-uptake-in-l6-myotubes-and-augments-insulin-secretion-from-pancreatic-beta-cells-by-activating-ampk
#7
Naomi Rozentul, Yosef Avrahami, Moran Shubely, Laura Levy, Anna Munder, Guy Cohen, Erol Cerasi, Shlomo Sasson, Arie Gruzman
PURPOSE: A series of novel polycyclic aromatic compounds that augment the rate of glucose uptake in L6 myotubes and increase glucose-stimulated insulin secretion from beta-cells were synthesized. Designing these molecules, we have aimed at the two main pathogenic mechanisms of T2D, deficient insulin secretion and diminished glucose clearance. The ultimate purpose of this work was to create a novel antidiabetic drug candidate with bi-functional mode of action. METHODS: All presented compounds were synthesized, and characterized in house...
October 5, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28968600/the-h-k-atpase-inhibitor-sch-28080-inhibits-insulin-secretion-and-induces-cell-death-in-ins-1e-rat-insulinoma-cells
#8
Martin Jakab, Nina Ketterl, Johannes Fürst, Marlena Beyreis, Michael Kittl, Tobias Kiesslich, Cornelia Hauser-Kronberger, Martin Gaisberger, Markus Ritter
BACKGROUND/AIMS: Glucose-stimulated insulin secretion (GSIS) of pancreatic β-cells involves glucose uptake and metabolism, closure of KATP channels and depolarization of the cell membrane potential (Vmem), activation of voltage-activated Ca2+ currents (ICav) and influx of Ca2+, which eventually triggers hormone exocytosis. Beside this classical pathway, KATP-independent mechanisms such as changes in intracellular pH (pHi) or cell volume, which also affect β-cell viability, can elicit or modify insulin release...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28911206/identification-of-the-molecular-dysfunction-caused-by-glutamate-dehydrogenase-s445l-mutation-responsible-for-hyperinsulinism-hyperammonemia
#9
Mariagrazia Grimaldi, Melis Karaca, Livia Latini, Estelle Brioudes, Thomas Schalch, Pierre Maechler
Congenital hyperinsulinism/hyperammonemia (HI/HA) syndrome gives rise to unregulated protein-induced insulin secretion from pancreatic beta-cells, fasting hypoglycemia and elevated plasma ammonia levels. Mutations associated with HI/HA were identified in the Glud1 gene, encoding for glutamate dehydrogenase (GDH). We aimed at identifying the molecular causes of dysregulation in insulin secretion and ammonia production conferred by the most frequent HI/HA mutation Ser445Leu. Following transduction with adenoviruses carrying the human GDH-wild type or GDH-S445L-mutant gene, immunoblotting showed efficient expression of the transgenes in all the investigated cell types...
September 15, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28833790/pycnogenol-cytotoxicity-in-pancreatic-ins-1e-%C3%AE-cells-induced-by-calcium-dysregulation
#10
Jana Viskupicova, Petronela Zizkova, Lucia Rackova, Lubica Horakova
Natural standardized flavonoid extract from the bark of Pinus pinaster, Pycnogenol (Pyc), was recently found to decrease intensively the activity of sarcoplasmic reticulum Ca(2+) -ATPase of rabbit skeletal muscle (SERCA1). On the basis of this inhibitory effect in a cell-free system and similarities of SERCA1 to its other isoforms, proapoptotic properties of Pyc may be expected in cellular systems. Pycnogenol (40-100 μg/mL) induced a concentration-dependent decrease of the viability of pancreatic INS-1E β cells associated with induction of apoptosis...
August 18, 2017: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/28767692/insulin-receptor-signaling-and-glucagon-like-peptide-1-effects-on-pancreatic-beta-cells
#11
Nunzia Caporarello, Cristina Parrino, Vincenzo Trischitta, Lucia Frittitta
Glucagon-like peptide-1 (GLP-1) is a potent gluco-incretin hormone, which plays a central role on pancreatic beta cell proliferation, survival and insulin secreting activity and whose analogs are used for treating hyperglycemia in type 2 diabetes mellitus. Notably, abnormal insulin signaling affects all the above-mentioned aspects on pancreatic beta cells. The aim of our study was to investigate whether the protective effects of GLP1-1 on beta cells are affected by altered insulin receptor signaling. To this end, several effects of GLP-1 were studied in INS-1E rat beta cells transfected either with an inhibitor of insulin receptor function (i...
2017: PloS One
https://www.readbyqxmd.com/read/28751022/er-resident-antioxidative-gpx7-and-gpx8-enzyme-isoforms-protect-insulin-secreting-ins-1e-%C3%AE-cells-against-lipotoxicity-by-improving-the-er-antioxidative-capacity
#12
Ilir Mehmeti, Stephan Lortz, Edward Avezov, Anne Jörns, Sigurd Lenzen
Increased circulating levels of saturated fatty acids (FFAs) and glucose are considered to be major mediators of β-cell dysfunction and death in T2DM. Although it has been proposed that endoplasmic reticulum (ER) and oxidative stress play a crucial role in gluco/lipotoxicity, their interplay and relative contribution to β-cell dysfunction and apoptosis has not been fully elucidated. In addition it is still unclear how palmitate - the physiologically most abundant long-chain saturated FFA - elicits ER stress and which immediate signals commit β-cells to apoptosis...
July 24, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28724742/the-myokine-irisin-is-released-in-response-to-saturated-fatty-acids-and-promotes-pancreatic-beta-cell-survival-and-insulin-secretion
#13
Annalisa Natalicchio, Nicola Marrano, Giuseppina Biondi, Rosaria Spagnuolo, Rossella Labarbuta, Immacolata Porreca, Angelo Cignarelli, Marco Bugliani, Piero Marchetti, Sebastio Perrini, Luigi Laviola, Francesco Giorgino
This study explored the role of irisin as a new pancreatic beta-cell secretagogue and survival factor and its potential role in the communication between skeletal muscle and pancreatic beta-cells under lipotoxic conditions. Recombinant irisin stimulated insulin biosynthesis and glucose-stimulated insulin secretion (GSIS) in a PKA-dependent manner, and prevented saturated fatty acid-induced apoptosis in human and rat pancreatic beta-cells, as well as in human and murine pancreatic islets, via AKT/BCL2 signaling...
July 19, 2017: Diabetes
https://www.readbyqxmd.com/read/28685559/both-3-5-diiodo-l-thyronine-t2-and-t3-modulate-glucose-induced-insulin-secretion
#14
P Fallahi, S M Ferrari, E Santini, S Camastra, G Frenzilli, M Puccini, F Goglia, A Lanni, P Marchetti, A Antonelli
3,5-diiodo-L-thyronine (T2), a naturally existing iodothyronine, has biological effects on humans, but no information is available on its action on pancreatic b-cells. We evaluated its effect vs triiodothyronine (T3), on glucose-induced insulin secretion in INS-1e cells, a rat insulinoma line, and on human islets. INS-1e were incubated in the presence/absence of T2 or T3 (0.1 nmol/L-10 μmol/L), and glucose (3.3, 7.5, 11.0, and 20 mmol/L). Insulin release and content (at 11.0 and 20 mmol/L glucose) were significantly (p less than 0...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28665109/role-of-site-specific-asparagine-deamidation-in-islet-amyloid-polypeptide-amyloidogenesis-key-contributions-of-residues-14-and-21
#15
Phuong Trang Nguyen, Ximena Zottig, Mathew Sebastiao, Steve Bourgault
Deamidation of an asparagine residue is a spontaneous non-enzymatic post-translational modification that results in the conversion of asparagine into a mixture of aspartic acid and isoaspartic acid. This chemical conversion modulates protein conformation and physicochemical properties, which could lead to protein misfolding and aggregation. In this study, we investigated the effects of site-specific Asn deamidation on the amyloidogenicity of the aggregation-prone peptide islet amyloid polypeptide (IAPP). IAPP is a 37-residue peptidic hormone whose deposition as insoluble amyloid fibrils is closely associated with type 2 diabetes...
July 25, 2017: Biochemistry
https://www.readbyqxmd.com/read/28527697/antibiotics-induce-mitonuclear-protein-imbalance-but-fail-to-inhibit-respiration-and-nutrient-activation-in-pancreatic-%C3%AE-cells
#16
Jaime Santo-Domingo, Isabelle Chareyron, Charlotte Broenimann, Steve Lassueur, Andreas Wiederkehr
Chloramphenicol and several other antibiotics targeting bacterial ribosomes inhibit mitochondrial protein translation. Inhibition of mitochondrial protein synthesis leads to mitonuclear protein imbalance and reduced respiratory rates as confirmed here in HeLa and PC12 cells. Unexpectedly, respiration in INS-1E insulinoma cells and primary human islets was unaltered in the presence of chloramphenicol. Resting respiratory rates and glucose stimulated acceleration of respiration were also not lowered when a range of antibiotics including, thiamphenicol, streptomycin, gentamycin and doxycycline known to interfere with bacterial protein synthesis were tested...
August 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28510423/phycocyanin-functionalized-selenium-nanoparticles-reverse-palmitic-acid-induced-pancreatic-%C3%AE-cell-apoptosis-by-enhancing-cellular-uptake-and-blocking-reactive-oxygen-species-ros-mediated-mitochondria-dysfunction
#17
Chang Liu, Yuanting Fu, Chang-E Li, Tianfeng Chen, Xiaoling Li
Accumulation of palmitic acid (PA) in human bodies could cause damage to pancreatic β cells and lead to chronic diseases by generation of reactive oxygen species (ROS). Therefore, it is of great significance to search for nutrition-available agents with antioxidant activity to protect pancreatic islet cells against PA-induced damage. Phycocyanin (PC) and selenium (Se) have been reported to have excellent antioxidant activity. In this study, PC-functionalized selenium nanoparticles (PC-SeNPs) were synthesized to investigate the in vitro protective effects on INS-1E rat insulinoma β cells against PA-induced cell death...
June 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28502980/w2476-ameliorates-%C3%AE-cell-dysfunction-and-exerts-therapeutic-effects-in-mouse-models-of-diabetes-via-modulation-of-the-thioredoxin-interacting-protein-signaling-pathway
#18
Ting Li, Guang-Yao Lin, Li Zhong, Yan Zhou, Jia Wang, Yue Zhu, Yang Feng, Xiao-Qing Cai, Qing Liu, Olivier Nosjean, Jean A Boutin, Pierre Renard, De-Hua Yang, Ming-Wei Wang
Recent evidence shows that high glucose levels recruit carbohydrate response element-binding protein, which binds the promoter of thioredoxin-interacting protein (txnip), thereby regulating its expression in β-cells. Overexpression of txnip not only induces β-cell apoptosis but also reduces insulin production. Thus, the discovery of compounds that either inhibit TXNIP activity or suppress its expression was the focus of the present study. INS-1E cells stably transfected with either a txnip proximal glucose response element connected to a luciferase reporter plasmid (BG73) or full-length txnip promoter connected to a luciferase reporter plasmid (CL108) were used in primary and secondary high-throughput screening campaigns, respectively...
July 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28449948/sunitinib-specifically-augments-glucose-induced-insulin-secretion
#19
Stefan Z Lutz, Axel Ullrich, Hans-Ulrich Häring, Susanne Ullrich, Felicia Gerst
The tyrosine kinase inhibitor sunitinib is used for the treatment of numerous cancers in humans. In diabetic patients, sunitinib lowers blood glucose levels and improves glycaemic control. This study aims to analyse whether sunitinib has specific and direct effects on insulin secreting β-cells. Regulation of insulin secretion, of cellular cAMP levels and activation of signalling pathways were examined upon exposure of rat insulinoma INS-1E cells to sunitinib under specific stimulatory and inhibitory conditions...
April 24, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28438776/the-immunoproteasome-is-induced-by-cytokines-and-regulates-apoptosis-in-human-islets
#20
Morten Lundh, Marco Bugliani, Tina Dahlby, Danny Hung-Chieh Chou, Bridget Wagner, Seyed Mojtaba Ghiasi, Vincenzo De Tata, Zhifei Chen, Marianne Nissan Lund, Michael J Davies, Piero Marchetti, Thomas Mandrup-Poulsen
In addition to degrading misfolded and damaged proteins, the proteasome regulates the fate of cells in response to stress. The role of the proteasome in pro-inflammatory cytokine-induced human beta-cell apoptosis is unknown. Using INS-1, INS-1E and human islets exposed to combinations of IFNγ, IL-1β and TNFα with or without addition of small molecules, we assessed the role of the immunoproteasome in pancreatic beta-cell demise. Here, we show that cytokines induce the expression and activity of the immuno-proteasome in INS-1E cells and human islets...
June 2017: Journal of Endocrinology
keyword
keyword
46765
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"