keyword
https://read.qxmd.com/read/38451906/dynamic-expression-of-cathepsin-l-in-the-black-soldier-fly-hermetia-illucens-gut-during-escherichia-coli-challenge
#21
JOURNAL ARTICLE
Yun-Ru Chiang, Han-Tso Lin, Chao-Wei Chang, Shih-Ming Lin, John Han-You Lin
The black soldier fly (BSF), Hermetia illucens, has the potential to serve as a valuable resource for waste bioconversion due to the ability of the larvae to thrive in a microbial-rich environment. Being an ecological decomposer, the survival of BSF larvae (BSFL) relies on developing an efficient defense system. Cathepsin L (CTSL) is a cysteine protease that plays roles in physiological and pathological processes. In this study, the full-length of CTSL was obtained from BSF. The 1,020-bp open reading frame encoded a preprotein of 339 amino acids with a predicted molecular weight of 32 kDa...
2024: PloS One
https://read.qxmd.com/read/38451450/syntaxin17-restores-lysosomal-function-and-inhibits-pyroptosis-caused-by-acinetobacter-baumannii
#22
JOURNAL ARTICLE
Zhiyuan An, Wenyi Ding
Acinetobacter baumannii (A. baumannii) causes autophagy flux disorder by degrading STX17, resulting in a serious inflammatory response. It remains unclear whether STX17 can alter the inflammatory response process by controlling autolysosome function. This study aimed to explore the role of STX17 in the regulation of pyroptosis induced by A. baumannii. Our findings indicate that overexpression of STX17 enhances autophagosome degradation, increases LAMP1 expression, reduces Cathepsin B release, and improves lysosomal function...
March 7, 2024: Journal of Microbiology / the Microbiological Society of Korea
https://read.qxmd.com/read/38451395/anti-rheumatoid-arthritis-potential-of-halodule-pinifolia-development-characterization-and-in-vivo-evaluation-of-h-pinifolia-based-oral-suspension-and-lipid-nano-emulsion
#23
JOURNAL ARTICLE
Samun Nessa, S Lavanya, Rajesh K Routray, Akash Chaurasiya, Onkar P Kulkarni, A Sajeli Begum
For treating chronic diseases like rheumatoid arthritis, herbal medicines are preferred due to their evident therapeutic effects and lesser side effects as compared to the long-term used conventional drugs. In this study, the anti-rheumatoid arthritis effect of an unexplored marine grass Halodule pinifolia (HP), and a combination of it with Glycyrrhiza glabra (liquorice; LQ), prepared as a conventional suspension (C1) and a lipid nano-emulsion (C1-N) was evaluated in Freund's complete adjuvant (FCA)- and collagen-induced arthritis (CIA) models...
March 7, 2024: Inflammopharmacology
https://read.qxmd.com/read/38445783/exosome-inhibiting-polymeric-sonosensitizer-for-tumor-specific-sonodynamic-immunotherapy
#24
JOURNAL ARTICLE
Jiayan Wu, Jingsheng Huang, Jie Yu, Mengke Xu, Jing Liu, Kanyi Pu
Combination cancer immunotherapy based on electromagnetic energy and immunotherapy has shown potent anti-cancer efficacy. However, as a factor that mediates tumor metastasis and immune suppression, the impact of tumor exosomes on therapy under electromagnetic energy stimulation remains unclear. Herein, our findings indicate that sonodynamic therapy (SDT) increases serum exosome levels by inducing apoptotic exosomes and loosening the tumor extracellular matrix, promoting lung metastasis. To address this problem, we synthesize an exosome-inhibiting polymeric sonosensitizer (EIPS) selectively inhibiting tumor exosome generation in response to the tumor biomarker...
March 6, 2024: Advanced Materials
https://read.qxmd.com/read/38445468/therapeutic-cysteine-protease-inhibitors-a-patent-review-2018-present
#25
REVIEW
Giulia Barchielli, Antonella Capperucci, Damiano Tanini
INTRODUCTION: Cysteine proteases are involved in a broad range of biological functions, ranging from extracellular matrix turnover to immunity. Playing an important role in the onset and progression of several diseases, including cancer, immune-related and neurodegenerative disease, viral and parasitic infections, cysteine proteases represent an attractive drug target for the development of therapeutic tools. AREAS COVERED: Recent scientific and patent literature focusing on the design and study of cysteine protease inhibitors with potential therapeutic application has been reviewed...
March 6, 2024: Expert Opinion on Therapeutic Patents
https://read.qxmd.com/read/38444130/-deficiency-of-cathepsin-k-improves-ischemic-angiogenesis-in-high-fat-diet-fed-mice
#26
JOURNAL ARTICLE
Xin-Yi Wang, Xu Wang, Zhen-Jie He, Shi-Piao Chen, Wei-Jian Li, Ren-Shan Cui, Hai-Ying Jiang
The present study aims to investigate the effect of cathepsin K (CatK) on ischemic angiogenesis in high-fat diet fed mice. The mice were subjected to unilateral hindlimb ischemic surgery, and the ischemic blood flow was measured with a laser Doppler blood flow imager. Immunohistochemical staining was used to observe the quantity of new capillaries in the ischemic lower extremity, and Western blot was used to detect the expression of insulin receptor substrate-1 (IRS-1), p-Akt, Akt and vascular endothelial growth factor (VEGF)...
February 25, 2024: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://read.qxmd.com/read/38443334/structure-based-design-of-pan-coronavirus-inhibitors-targeting-host-cathepsin-l-and-calpain-1
#27
JOURNAL ARTICLE
Xiong Xie, Qiaoshuai Lan, Jinyi Zhao, Sulin Zhang, Lu Liu, Yumin Zhang, Wei Xu, Maolin Shao, Jingjing Peng, Shuai Xia, Yan Zhu, Keke Zhang, Xianglei Zhang, Ruxue Zhang, Jian Li, Wenhao Dai, Zhen Ge, Shulei Hu, Changyue Yu, Jiang Wang, Dakota Ma, Mingyue Zheng, Haitao Yang, Gengfu Xiao, Zihe Rao, Lu Lu, Leike Zhang, Fang Bai, Yao Zhao, Shibo Jiang, Hong Liu
Respiratory disease caused by coronavirus infection remains a global health crisis. Although several SARS-CoV-2-specific vaccines and direct-acting antivirals are available, their efficacy on emerging coronaviruses in the future, including SARS-CoV-2 variants, might be compromised. Host-targeting antivirals provide preventive and therapeutic strategies to overcome resistance and manage future outbreak of emerging coronaviruses. Cathepsin L (CTSL) and calpain-1 (CAPN1) are host cysteine proteases which play crucial roles in coronaviral entrance into cells and infection-related immune response...
March 6, 2024: Signal Transduction and Targeted Therapy
https://read.qxmd.com/read/38439203/hepatic-and-adipose-tissue-transcriptome-analysis-highlights-a-commonly-deregulated-autophagic-pathway-in-severe-masld
#28
JOURNAL ARTICLE
Marica Meroni, Emilia De Caro, Federica Chiappori, Miriam Longo, Erika Paolini, Ettore Mosca, Ivan Merelli, Rosa Lombardi, Sara Badiali, Marco Maggioni, Alessandro Orro, Alessandra Mezzelani, Luca Valenti, Anna Ludovica Fracanzani, Paola Dongiovanni
OBJECTIVE: The incidence of metabolic dysfunction-associated steatotic liver disease (MASLD) is rapidly ramping up due to the spread of obesity, which is characterized by expanded and dysfunctional visceral adipose tissue (VAT). Previous studies have investigated the hepatic transcriptome across MASLD, whereas few studies have focused on VAT. METHODS: We performed RNA sequencing in 167 hepatic samples from patients with obesity and in a subset of 79 matched VAT samples...
March 4, 2024: Obesity
https://read.qxmd.com/read/38433822/novel-bisphosphonate-based-cathepsin-k-triggered-compound-targets-the-enthesis-without-impairing-soft-tissue-to-bone-healing
#29
JOURNAL ARTICLE
Brendan Y Shi, Varun Sriram, Shannon Y Wu, Dave Huang, Alexis Cheney, Melodie F Metzger, Oskar Sundberg, Karen M Lyons, Charles E McKenna, Ichiro Nishimura, Thomas J Kremen
Background: Osteoadsorptive fluorogenic sentinel 3 (OFS-3) is a recently described compound that contains a bone-targeting bisphosphonate (BP) and cathepsin K (Ctsk)-triggered fluorescence signal. A prior study in a murine Achilles repair model demonstrated its effectiveness at targeting the site of tendon-to-bone repair, but the intrinsic effect of this novel bisphosphonate chaperone on tendon-to-bone healing has not been previously explored. We hypothesized that application of this bisphosphonate-fluorophore cargo conjugate would not affect the biomechanical properties or histologic appearance of tendon-bone repairs...
2024: Frontiers in Bioengineering and Biotechnology
https://read.qxmd.com/read/38432290/cytoskeletal-%C3%AE-tubulin-and-cysteine-cathepsin-l-deregulation-by-sars-cov-2-spike-protein-interaction-with-the-neuronal-model-cell-line-sh-sy5y
#30
JOURNAL ARTICLE
Bernardo R Oliveira, Inga Nehlmeier, Amy Madeleine Kempf, Vaishnavi Venugopalan, Maren Rehders, Marianne E P Ceniza, Pedro A de T P V Cavalcanti, Markus Hoffmann, Stefan Pöhlmann, Klaudia Brix
SARS-CoV-2 mainly infects the respiratory tract but can also target other organs, including the central nervous system. While it was recently shown that cells of the blood-brain-barrier are permissive to SARS-CoV-2 infection in vitro, it remains debated whether neurons can be infected. In this study, we demonstrate that vesicular stomatitis virus particles pseudotyped with the spike protein of SARS-CoV-2 variants WT, Alpha, Delta and Omicron enter the neuronal model cell line SH-SY5Y. Cell biological analyses of the pseudo-virus treated cultures showed marked alterations in microtubules of SH-SY5Y cells...
March 1, 2024: Biochimie
https://read.qxmd.com/read/38431778/pdx-proteins-from-arabidopsis-thaliana-as-novel-substrates-of-cathepsin-b-implications-for-vitamin-b-6-biosynthesis-regulation
#31
JOURNAL ARTICLE
Marcelina Parra, Marianna Coppola, Hanjo Hellmann
Vitamin B6 is a critical molecule for metabolism, development, and stress sensitivity in plants. It is a cofactor for numerous biochemical reactions, can serve as an antioxidant, and has the potential to increase tolerance against both biotic and abiotic stressors. Due to the importance of vitamin B6 , its biosynthesis is likely tightly regulated. Plants can synthesize vitamin B6 de novo via the concerted activity of Pyridoxine Biosynthesis Protein 1 (PDX1) and PDX2. Previously, PDX proteins have been identified as targets for ubiquitination, indicating they could be marked for degradation by two highly conserved pathways: the Ubiquitin Proteasome Pathway (UPP) and the autophagy pathway...
March 3, 2024: FEBS Journal
https://read.qxmd.com/read/38431208/the-role-of-multiple-vitellogenins-in-early-development-of-fishes
#32
JOURNAL ARTICLE
Yilmaz Ozlem, V Sullivan Craig, Bobe Julien, Norberg Birgitta
Functions of vitellogenins have been in the limelight of fish reproductive physiology research for decades. The Vtg system of acanthomorph teleosts consists of two complete forms of Vtgs (VtgAa and VtgAb) and an incomplete form, VtgC. Insufficient uptake and processing of Vtgs and their yolk proteins lead to inadequate oocyte hydration ensuing failure in acquisition of egg buoyancy and early developmental deficiencies. This review presents a summary of our studies on utilization of multiple Vtgs in species with different egg buoyancy characteristics, as examples...
February 29, 2024: General and Comparative Endocrinology
https://read.qxmd.com/read/38428068/agcm-22-a-novel-cetuximab-based-egfr-targeting-antibody-drug-conjugate-with-highly-selective-anti-glioblastoma-efficacy
#33
JOURNAL ARTICLE
Dapeng Li, Xianyan Sun, Yiquan Li, Chao Shang, Yuchao Dong, Renshuang Zhao, Hang Zhang, Zihao Wang, Shiyong Fan, Chengyuan Ma, Xiao Li
The epidermal growth factor receptor (EGFR) has received significant attention as a potential target for glioblastoma (GBM) therapeutics in the past two decades. However, although cetuximab, an antibody that specifically targets EGFR, exhibits a high affinity for EGFR, it has not yet been applied in the treatment of GBM. Antibody-drug conjugates (ADCs) utilize tumor-targeting antibodies for the selective delivery of cytotoxic drugs, resulting in improved efficacy compared to conventional chemotherapy drugs...
February 27, 2024: Bioorganic & Medicinal Chemistry
https://read.qxmd.com/read/38427138/adiponectin-attenuates-h2o2-induced-apoptosis-in-chicken-skeletal-myoblasts-through-the-lysosomal-mitochondrial-axis
#34
JOURNAL ARTICLE
Han Wang, Chi Li, Longbo Zhu, Zhengqun Liu, Ning Li, Zi Zheng, Shiyue Liang, Jun Yan
Adiponectin has previously been investigated for exerting its protective effect against myocardial injury through anti-apoptotic and anti-oxidative actions. Therefore, the present study aimed to investigate the nature and mechanism of adiponectin inhibition of H2 O2 -induced apoptosis in chicken skeletal myoblasts. Skeletal muscle satellite cells were differentiated and assigned into three groups. Group C was on the blank control group, group H was stimulated with the H2 O2 (500 μmol/L, 4 h) alone group, group A + H was pre-treated with adiponectin (10 μg/mL, 24 h) and stimulated with the H2 O2 (500 μmol/L, 4 h) group...
February 26, 2024: In Vitro Cellular & Developmental Biology. Animal
https://read.qxmd.com/read/38426210/proteomic-analyses-of-urinary-exosomes-identify-novel-potential-biomarkers-for-early-diagnosis-of-sickle-cell-nephropathy-a-sex-based-study
#35
JOURNAL ARTICLE
Balamurugan Packialakshmi, Emily Limerick, Hans C Ackerman, Xionghao Lin, Sergei Nekhai, James D Oliver, Ian J Stewart, Mark A Knepper, Courtney Fitzhugh, Xiaoming Zhou
Sickle cell nephropathy (SCN) is a leading cause of morbidity and mortality in sickle cell disease (SCD). Early intervention is crucial for mitigating its effects. However, current diagnostic methods rely on generic tests and may not detect SCN until irreversible renal damage occurs. Therefore, specific biomarkers for early diagnosis of SCN are needed. Urinary exosomes, membrane-bound vesicles secreted by renal podocytes and epithelial cells, contain both common and cell type-specific membrane and cytosolic proteins, reflecting the physiologic and pathophysiologic states of the kidney...
2024: Frontiers in Physiology
https://read.qxmd.com/read/38422876/the-size-dependence-and-reversibility-of-polystyrene-nanoplastics-induced-lipid-accumulation-in-mice-possible-roles-of-lysosomes
#36
JOURNAL ARTICLE
Yan-Yang Lu, Lu Lu, Hong-Yun Ren, Weizhen Hua, Nengxing Zheng, Fu-Yi Huang, Jiani Wang, Meiping Tian, Qingyu Huang
Nanoplastics (NPs) continue to accumulate in global aquatic and terrestrial systems, posing a potential threat to human health through the food chain and/or other pathways. Both in vivo and in vitro studies have confirmed that the liver is one of the main organs targeted for the accumulation of NPs in living organisms. However, whether exposure to NPs induces size-dependent disorders of liver lipid metabolism remains controversial, and the reversibility of NPs-induced hepatotoxicity is largely unknown. In this study, the effects of long-term exposure to environmentally relevant doses of polystyrene nanoplastics (PS-NPs) on lipid accumulation were investigated in terms of autophagy and lysosomal mechanisms...
February 24, 2024: Environment International
https://read.qxmd.com/read/38420756/monocytes-release-pro-cathepsin-d-to-drive-blood-to-brain-transcytosis-in-diabetes
#37
JOURNAL ARTICLE
Dan Zhao, Zeng-Kang Huang, Yu Liang, Zhi-Jun Li, Xue-Wei Zhang, Kun-Hang Li, Hao Wu, Xu-Dong Zhang, Chen-Sheng Li, Dong An, Xue Sun, Ming-Xin An, Jun-Xiu Shi, Yi-Jun Bao, Li Tian, Di-Fei Wang, An-Hua Wu, Yu-Hua Chen, Wei-Dong Zhao
BACKGROUND: Microvascular complications are the major outcome of type 2 diabetes progression, and the underlying mechanism remains to be determined. METHODS: High-throughput RNA sequencing was performed using human monocyte samples from controls and diabetes. The transgenic mice expressing human CTSD (cathepsin D) in the monocytes was constructed using CD68 promoter. In vivo 2-photon imaging, behavioral tests, immunofluorescence, transmission electron microscopy, Western blot analysis, vascular leakage assay, and single-cell RNA sequencing were performed to clarify the phenotype and elucidate the molecular mechanism...
February 29, 2024: Circulation Research
https://read.qxmd.com/read/38420467/sars-cov-2-mechanisms-of-cell-tropism-in-various-organs-considering-host-factors
#38
REVIEW
Emad Behboudi, Seyed Nooreddin Faraji, Gholamreza Daryabor, Seyed Mohammad Ali Hashemi, Maryam Asadi, Fahime Edalat, Mohammad Javad Raee, Gholamreza Hatam
A critical step in the drug design for SARS-CoV-2 is to discover its molecular targets. This study comprehensively reviewed the molecular mechanisms of SARS-CoV-2, exploring host cell tropism and interaction targets crucial for cell entry. The findings revealed that beyond ACE2 as the primary entry receptor, alternative receptors, co-receptors, and several proteases such as TMPRSS2, Furin, Cathepsin L, and ADAM play critical roles in virus entry and subsequent pathogenesis. Additionally, SARS-CoV-2 displays tropism in various human organs due to its diverse receptors...
February 29, 2024: Heliyon
https://read.qxmd.com/read/38418607/cathepsin-b-degrades-rbcl-during-freezing-induced-programmed-cell-death-in-arabidopsis
#39
JOURNAL ARTICLE
Gang Yang, Tao Chen, Ting-Ting Fan, Xiang-Yu Lin, Yu-Qiong Cui, Wei-Chao Dong, Li-Zhe An, Hua Zhang
Cathepsin B plays an important role that degrades the Rubisco large subunit RbcL in freezing stress. Programmed cell death (PCD) has been well documented in both development and in response to environmental stresses in plants, however, PCD induced by freezing stress and its molecular mechanisms remain poorly understood. In the present study, we characterized freezing-induced PCD and explored its mechanisms in Arabidopsis. PCD induced by freezing stress was similar to that induced by other stresses and senescence in Arabidopsis plants with cold acclimation...
February 28, 2024: Plant Cell Reports
https://read.qxmd.com/read/38414787/anti%C3%A2-osteoclastogenic-effect-of-fermented-mealworm-extract-by-inhibiting-rankl%C3%A2-induced-nfatc1-action
#40
JOURNAL ARTICLE
Ju Ri Ham, Mi-Kyung Lee
Augmented osteoclast activity and differentiation can lead to destructive bone diseases, such as arthritis and osteoporosis. Therefore, modulating osteoclastogenesis and differentiation may serve to be a possible strategy for treating such diseases. Tenebrio molitor larvae, also known as mealworms, are considered a good source of protein with nutritional value, digestibility, flavor and functional properties, such as antioxidant, anti-diabetic and anti-obesity effects. However, the role of mealworms in osteoclastogenesis remains poorly understood...
April 2024: Experimental and Therapeutic Medicine
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