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https://www.readbyqxmd.com/read/29139030/potential-risk-factors-for-reactive-arthritis-and-persistence-of-symptoms-at-2%C3%A2-years-a-case-control-study-with-longitudinal-follow-up
#1
Helga Raquel Garcia Ferrer, Alexander Azan, Isa Iraheta, Joan Von Feldt, Luis R Espinoza, Julia Manasson, Jose U Scher, Abraham Garcia Kutzbach, Alexis Ogdie
The objective of the study is to determine the risk factors for the development of reactive arthritis (ReA) and examine the factors associated with the persistence of symptoms. Patients with a new diagnosis of ReA and controls with a gastrointestinal (GI), urogenital, or sexually transmitted infection in the 3-6 months prior to study entry were prospectively enrolled in Guatemala City. ReA patients fulfilled the Assessment in Spondyloarthritis International Society criteria for peripheral spondyloarthropathy (SpA)...
November 14, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/29129636/inflammation-mediated-muscle-metabolic-dysregulation-local-and-remote-to-the-site-of-major-abdominal-surgery
#2
Krishna K Varadhan, Dumitru Constantin-Teodosiu, Despina Constantin, Paul L Greenhaff, Dileep N Lobo
BACKGROUND & AIMS: Postoperative hyperglycaemia is common in patients having major surgery and is associated with adverse outcomes. This study aimed to determine whether bacteraemia contributed to postoperative systemic inflammation, and whether increases in the expression of muscle mRNAs and proteins reflecting increased muscle inflammation, atrophy and impaired carbohydrate oxidation were evident at the time of surgery, and both local and distant to the site of trauma, and could be associated with impaired glucoregulation...
November 2, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/29128403/cln5-is-secreted-and-functions-as-a-glycoside-hydrolase-in-dictyostelium
#3
Robert J Huber, Sabateeshan Mathavarajah
Ceroid lipofuscinosis neuronal 5 (CLN5) is a member of a family of proteins that are linked to neuronal ceroid lipofuscinosis (NCL). This devastating neurological disorder, known commonly as Batten disease, affects all ages and ethnicities and is currently incurable. The precise function of CLN5, like many of the NCL proteins, remains to be elucidated. In this study, we report the localization, molecular function, and interactome of Cln5, the CLN5 homolog in the social amoeba Dictyostelium discoideum. Residues that are glycosylated in human CLN5 are conserved in the Dictyostelium homolog as are residues that are mutated in patients with CLN5 disease...
November 8, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/29126020/mechanistic-insight-in-potential-dual-role-of-sinigrin-against-helicoverpa-armigera
#4
Aniruddha R Agnihotri, Chaitanya V Hulagabali, Anmol S Adhav, Rakesh S Joshi
The adverse effect of glucosinolates on diverse phytophagous insects is well documented, but its impact on insect physiology has remained enigmatic. Here we report insights into detrimental effects of plant glucosinolate molecule, sinigrin, on Helicoverpa armigera growth and development. In-silico screening of multiple glucosinolates predicted sinigrin as one of the potential inhibitor of H. armigera cathepsin B and L. Insects fed on sinigrin containing diet showed significantly reduced growth (20-30%), delayed pupation (10-15%), decreased fecundity (50-80%) and developmental abnormalities...
November 7, 2017: Phytochemistry
https://www.readbyqxmd.com/read/29123184/urinary-peptidomics-analysis-reveals-proteases-involved-in-diabetic-nephropathy
#5
Magdalena Krochmal, Georgia Kontostathi, Pedro Magalhães, Manousos Makridakis, Julie Klein, Holger Husi, Johannes Leierer, Gert Mayer, Jean-Loup Bascands, Colette Denis, Jerome Zoidakis, Petra Zürbig, Christian Delles, Joost P Schanstra, Harald Mischak, Antonia Vlahou
Mechanisms underlying the onset and progression of nephropathy in diabetic patients are not fully elucidated. Deregulation of proteolytic systems is a known path leading to disease manifestation, therefore we hypothesized that proteases aberrantly expressed in diabetic nephropathy (DN) may be involved in the generation of DN-associated peptides in urine. We compared urinary peptide profiles of DN patients (macroalbuminuric, n = 121) to diabetic patients with no evidence of DN (normoalbuminuric, n = 118)...
November 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29123088/a-water-soluble-nucleolin-aptamer-paclitaxel-conjugate-for-tumor-specific-targeting-in-ovarian-cancer
#6
Fangfei Li, Jun Lu, Jin Liu, Chao Liang, Maolin Wang, Luyao Wang, Defang Li, Houzong Yao, Qiulong Zhang, Jia Wen, Zong-Kang Zhang, Jie Li, Quanxia Lv, Xiaojuan He, Baosheng Guo, Daogang Guan, Yuanyuan Yu, Lei Dang, Xiaohao Wu, Yongshu Li, Guofen Chen, Feng Jiang, Shiguo Sun, Bao-Ting Zhang, Aiping Lu, Ge Zhang
Paclitaxel (PTX) is among the most commonly used first-line drugs for cancer chemotherapy. However, its poor water solubility and indiscriminate distribution in normal tissues remain clinical challenges. Here we design and synthesize a highly water-soluble nucleolin aptamer-paclitaxel conjugate (NucA-PTX) that selectively delivers PTX to the tumor site. By connecting a tumor-targeting nucleolin aptamer (NucA) to the active hydroxyl group at 2' position of PTX via a cathepsin B sensitive dipeptide bond, NucA-PTX remains stable and inactive in the circulation...
November 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/29121463/determining-the-toxicity-of-rare-earth-oxide-nanomaterials-in-macrophages-and-the-involvement-of-membrane-damage
#7
Jie Gao, Ruibin Li, Fengbang Wang, Xiaolei Liu, Jie Zhang, Ligang Hu, Jianbo Shi, Bin He, Qunfang Zhou, Maoyong Song, Bin Zhang, Guangbo Qu, Sijin Liu, Guibin Jiang
Rare earth oxide nanomaterials (REE NPs) hold considerable promise, with high availability and potential applications as superconductors, imaging agents, glass additives, fertilizers additives and feed additives. These results in potential REE NP exposure to humans and the environment through different routes and adverse effects induced by biological application of these materials are becoming an increasing concern. This study investigates the cytotoxicity of REE NPs: nLa2O3, nEu2O3, nDy2O3 and nYb2O3 from 2...
November 9, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29120254/novel-compound-heterozygous-mutations-causing-kufs-disease-type-b
#8
Cui Wang, Hongliang Xu, Yun Yuan, Yajun Lian, Nanchang Xie, Liang Ming
Kufs disease (KD) type B (also termed CLN13), an adult-onset form of neuronal ceroid lipofuscinosis (NCL), is genetically heterogeneous and challenging to diagnose. Recently, mutations in cathepsin-F (CTSF) have been identified as the causative gene for autosomal recessive KD type B. Here, we report a sporadic case of KD type B with novel compound heterozygous mutations, a novel missense mutation c.977G>T (p.C326F) and a novel nonsense mutation c.416C>A (p.S139X), in the CTSF gene. The magnetic resonance imaging findings were consistent with those demonstrated in adult NCL: diffuse cortical atrophy, mild hyperintensity and reduction of the deep white matter on T2-weighted images...
November 9, 2017: International Journal of Neuroscience
https://www.readbyqxmd.com/read/29114958/evidence-for-multiple-modes-of-neutrophil-serine-protease-recognition-by-the-eap-family-of-staphylococcal-innate-immune-evasion-proteins
#9
Daphne A C Stapels, Jordan L Woehl, Fin J Milder, Angelino T Tromp, Aernoud A van Batenburg, Wilco C de Graaf, Samuel C Broll, Natalie M White, Suzan H M Rooijakkers, Brian V Geisbrecht
Neutrophils contain high levels of chymotrypsin-like serine proteases (NSPs) within their azurophilic granules that have a multitude of functions within the immune system. In response, the pathogen Staphylococcus aureus has evolved three potent inhibitors (Eap, EapH1, and EapH2) that protect the bacterium as well as several of its secreted virulence factors from the degradative action of NSPs. We previously showed that these so-called EAP domain proteins represent a novel class of NSP inhibitors characterized by a non-covalent inhibitory mechanism and a distinct target specificity profile...
November 7, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29113862/comparative-expression-analysis-of-immune-related-factors-in-the-sea-cucumber-apostichopus-japonicus
#10
Jingwei Jiang, Zunchun Zhou, Ying Dong, Zelong Zhao, Hongjuan Sun, Bai Wang, Bei Jiang, Zhong Chen, Shan Gao
In order to preliminarily explore the joint involvement of different immune-related factors during the same immune process in Apostichopus japonicus, the transcriptional expression of Cu/Zn superoxide dismutase (Cu/Zn-SOD), catalase (CAT), c-type lysozyme (c-LYZ), i-type lysozyme (i-LYZ), cathepsin D, melanotransferrin (MTF), Toll, c-type lectin (c-LCT) and complement 3 (C3) during the development from fertilized eggs to juveniles and after challenging the juveniles with Vibrio splendidus, Pseudoalteromonas nigrifaciens, Shewanella baltica and Bacillus cereus, respectively, was measured using the method of quantitative real-time PCR (qRT-PCR), and then the correlations among different immune-related factors were analyzed...
November 4, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29109534/natural-history-of-helicobacter-pylori-vaca-toxin-in-human-gastric-epithelium-in-vivo-vacuoles-and-beyond
#11
Vittorio Necchi, Patrizia Sommi, Alessandro Vanoli, Roberto Fiocca, Vittorio Ricci, Enrico Solcia
Uptake, intracellular trafficking and pathologic effects of VacA toxin from Helicobacter pylori have been widely investigated in vitro. However, no systematic analysis investigated VacA intracellular distribution and fate in H. pylori-infected human gastric epithelium in vivo, using ultrastructural immunocytochemistry that combines precise toxin localization with analysis of the overall cell ultrastructure and intercompartimental/interorganellar relationships. By immunogold procedure, in this study we investigated gastric biopsies taken from dyspeptic patients to characterize the overall toxin's journey inside human gastric epithelial cells in vivo...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29107736/absence-of-vitamin-d-receptor-in-mature-osteoclasts-results-in-altered-osteoclastic-activity-and-bone-loss
#12
Yolandi Starczak, Daniel C Reinke, Kate R Barratt, Jackson W Ryan, René St-Arnaud, Howard A Morris, Rachel A Davey, Gerald J Atkins, Paul H Anderson
Mature osteoclasts express the vitamin D receptor (VDR) and are able to synthesise and respond to 1,25(OH)2D3 via CYP27B1 enzyme activity. Whether vitamin D signalling within osteoclasts is necessary for the regulation of osteoclastic bone resorption in an in vivo setting is unclear. To determine the requirement for the VDR- and CYP27B1-mediated activity in mature osteoclasts, conditional deletion mouse models were created whereby either Vdr or Cyp27b1 gene was inactivated by breeding either Vdr(fl/fl) or Cyp27b1(fl/fl) mice with Cathepsin K-Cre transgenic mice (Cstk(Cre)) to generate Ctsk(Cre)/Vdr(-/-) and Ctsk(Cre)/Cyp27b1(-/-) mice respectively...
October 28, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29107144/osteon-myospalacem-baileyi-attenuates-osteoclast-differentiation-through-rankl-induced-nfat-pathways
#13
Yulei Cui, Xiaoying Zhao, Lijuan Mei, Jinjin Pei, Shuo Wang, Yun Shao, Yanduo Tao, Xiaoling Zhang, Lei Jiang
ETHNOPHARMACOLOGICAL RELEVANCE: Osteon Myospalacem Baileyi, known as Sai long gu (Tibetan language, means "blind rat bone"), is the whole skeleton of Tibet plateau rodentia animal Myospalacem Baileyi. Osteon Myospalacem Baileyi had been widely used in the Tibet region as an anti-osteoporosis drug and since 1991 Osteon Myospalacem Baileyi has been listed in the Pharmacopoeia of People's Republic of China as the first-class animal new medical material. However, the mechanism of its anti-osteoporosis activities is still unclear...
October 26, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29104112/unclassified-hemangioma-like-renal-cell-carcinoma-a-potential-diagnostic-pitfall
#14
Kanika Taneja, Sohrab Arora, Craig G Rogers, Nilesh S Gupta, Liang Cheng, Sean R Williamson
Recently, rare renal cell carcinomas (RCC) have been reported to closely mimic hemangioma; however, these have been largely recognizable as clear cell RCC. Conversely, true hemangiomas of the kidney are also increasingly recognized. We report a 62-year-old woman who underwent partial nephrectomy for a hemangioma-like RCC without appreciable clear cell morphology. Immunohistochemistry revealed luminal structures that stained positively for cytokeratin (CK), CK7, carbonic anhydrase IX, PAX8, and high molecular weight keratin (HMWK), admixed with a CD34, CD31, and ERG-positive complex network of vessels...
November 2, 2017: Human Pathology
https://www.readbyqxmd.com/read/29104086/an-easy-to-use-fret-protein-substrate-to-detect-calpain-cleavage-in-vitro-and-in-vivo
#15
Christian-Scott E McCartney, James A MacLeod, Peter A Greer, Peter L Davies
Calpain-1 and -2 are Ca(2+)-activated intracellular cysteine proteases that regulate a wide range of cellular functions through the cleavage of their protein substrates. Unlike degradative proteases, calpains make limited, transformative cleavages, typically in accessible sequences linking discrete subdomains, to irreversibly alter substrate functions. The biological roles of calpain and their interplay with calcium signaling are of significant biomedical interest as biomarkers and potential therapeutic targets in a growing number of diseases including Alzheimer's, cancer and fibrosis...
November 2, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29103265/dna-vaccines-encoding-toxoplasma-gondii-cathepsin-c-1-induce-protection-against-toxoplasmosis-in-mice
#16
Yali Han, Aihua Zhou, Gang Lu, Guanghui Zhao, Wenchao Sha, Lin Wang, Jingjing Guo, Jian Zhou, Huaiyu Zhou, Hua Cong, Shenyi He
Toxoplasma gondii cathepsin C proteases (TgCPC1, 2, and 3) are important for the growth and survival of T. gondii. In the present study, B-cell and T-cell epitopes of TgCPC1 were predicted using DNAstar and the Immune Epitope Database. A TgCPC1 DNA vaccine was constructed, and its ability to induce protective immune responses against toxoplasmosis in BALB/c mice was evaluated in the presence or absence of the adjuvant α-GalCer. As results, TgCPC1 DNA vaccine with or without adjuvant α-GalCer showed higher levels of IgG and IgG2a in the serum, as well as IL-2 and IFN-γ in the spleen compared to controls (PBS, pEGFP-C1, and α-Galcer)...
October 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/29102662/rip1k-contributes-to-neuronal-and-astrocytic-cell-death-in-ischemic-stroke-via-activating-autophagic-lysosomal-pathway
#17
Yong Ni, Wei-Wei Gu, Zhi-He Liu, Yong-Ming Zhu, Jia-Guo Rong, Thomas A Kent, Min Li, Shi-Gang Qiao, Jian-Zhong An, Hui-Ling Zhang
Although the receptor-interacting protein 1 kinase (RIP1K)-regulated necroptosis can be evoked by cerebral ischemia, the effects of RIP1K in mediating neuronal and astrocytic cell death and the underlying mechanisms remain poorly understood. This study evaluates the contribution of RIP1K to ischemic stroke-induced neuronal and astrocytic cell death, and the activation of autophagic-lysosomal pathway. Using an in vitro oxygen and glucose deprivation (OGD) in primary cultured neurons or astrocytes and a permanent middle cerebral artery occlusion (pMCAO) model in rats or mice, we observed the role of RIP1K in the ischemic neuronal and astrocytic cell death and the underlying mechanisms by pharmacological or genetic inhibition of RIP1K...
November 8, 2017: Neuroscience
https://www.readbyqxmd.com/read/29101342/a-protease-resistant-escherichia-coli-asparaginase-with-outstanding-stability-and-enhanced-anti-leukaemic-activity-in-vitro
#18
Maristella Maggi, Steven D Mittelman, Jean Hugues Parmentier, Giorgio Colombo, Massimiliano Meli, Jeannette Marie Whitmire, D Scott Merrell, Julian Whitelegge, Claudia Scotti
L-Asparaginases (ASNases) have been used as first line drugs for paediatric Acute Lymphoblastic Leukaemia (ALL) treatment for more than 40 years. Both the Escherichia coli (EcAII) and Erwinia chrysanthemi (ErAII) type II ASNases currently used in the clinics are characterized by high in vivo instability, short half-life and the requirement of several administrations to obtain a pharmacologically active concentration. Moreover, they are sensitive to proteases (cathepsin B and asparagine endopeptidase) that are over-expressed by resistant leukaemia lymphoblasts, thereby impairing drug activity and pharmacokinetics...
November 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29100330/protective-cellular-immunity-generated-by-cross-presenting-recombinant-overlapping-peptide-proteins
#19
Lili Cai, Jianbo Zhang, Renying Zhu, Weixing Shi, Xiaobing Xia, Mark Edwards, William Finch, Anthony Coombs, Ju Gao, Kangwen Chen, Sophie Owen, Shisong Jiang, Wenshu Lu
Priming of naive CD8(+) and CD4(+) T cells by dendritic cells (DCs) requires effective antigen presentation on both MHC class I and II molecules. We have developed a novel technology to use recombinant overlapping peptides (ROP) that stimulate both CD8(+) and CD4(+) T cell immune responses. The single chain protein of a ROP is made up of overlapping peptides linked by the target sequence (LRMK) for cathepsin S, a protease found in the endosomes of DCs. We designed synthetic genes encoding ROPs derived from ovalbumin (OVA), tuberculosis protein (CFP10-ESAT6), human papilloma virus (HPV) protein (E7) and survivin, a protein commonly over-expressed in tumour cells...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29096286/effects-of-single-nucleotide-polymorphisms-and-haplotypes-of-the-protein-kinase-amp-activated-non-catalytic-subunit-gamma-3-prkag3-gene-on-production-meat-quality-and-carcass-traits-in-italian-large-white-pigs
#20
Stefania Dall'Olio, Emilio Scotti, Leonardo Nanni Costa, Luca Fontanesi
PRKAG3 is a major gene for meat quality and production in pigs. In this study, we investigated two single nucleotide polymorphisms (SNPs) of the PRKAG3 promoter region (g.-995A>G and g.-311A>G) in 381 pigs of different breeds. Association between these SNPs and three major haplotypes, constructed including other three substitutions (c.89C>A, c.154A>G and c.595G>A) and different traits (meat quality parameters: pH2h, pHu, glycogen and lactate content, glycolytic potential and cathepsin B activity of Semimembranosus muscle; estimated breeding values and random residuals for growth performances and carcass traits) were analysed in 271 Italian Large White performance tested pigs...
September 25, 2017: Meat Science
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