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https://www.readbyqxmd.com/read/29150519/biological-containment-of-genetically-modified-bacillus-subtilis
#1
Siamand Hosseini, Alex Curilovs, Simon M Cutting
Genetic manipulation of bacterial spores of the genus Bacillus has shown potential for vaccination and for delivery of drugs or enzymes. Remarkably, proteins displayed on the spore surface retain activity and generally are not degraded. The heat stability of spores coupled with their desiccation resistance makes them suitable for delivery to humans or to animals by the oral route. Despite these attributes one regulatory obstacle has remained regarding the fate of recombinant spores shed into the environment as viable spores...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29147107/debated-agronomy-public-discourse-and-the-future-of-biotechnology-policy-in-ghana
#2
Joseph A Braimah, Kilian N Atuoye, Siera Vercillo, Carrie Warring, Isaac Luginaah
This paper examines the highly contested and ongoing biotechnology (Bt) policy-making process in Ghana. We analyse media content on how Bt is viewed in the context of Ghana's parliamentary debate on the Plant Breeders Bill and within the broader public policy-making literature. This paper does not seek to take a position on Bt or the Bill, but to understand how policy actors influence the debate with political and scientific rhetoric in Ghana. The study reveals that in the midst of scientific uncertainties of Bt's potential for sustainable agriculture production and food security, policy decisions that encourage its future adoption are heavily influenced by health, scientific, economic, environmental and political factors dictated by different ideologies, values and norms...
2017: Global Bioethics, Problemi di Bioetica
https://www.readbyqxmd.com/read/29110175/optimization-and-verification-of-droplet-digital-pcr-even-specific-methods-for-the-quantification-of-gm-maize-das1507-and-nk603
#3
Katarzyna Grelewska-Nowotko, Magdalena Żurawska-Zajfert, Ewelina Żmijewska, Sławomir Sowa
In recent years, digital polymerase chain reaction (dPCR), a new molecular biology technique, has been gaining in popularity. Among many other applications, this technique can also be used for the detection and quantification of genetically modified organisms (GMOs) in food and feed. It might replace the currently widely used real-time PCR method (qPCR), by overcoming problems related to the PCR inhibition and the requirement of certified reference materials to be used as a calibrant. In theory, validated qPCR methods can be easily transferred to the dPCR platform...
November 6, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29074984/alf-a-strategy-for-identification-of-unauthorized-gmos-in-complex-mixtures-by-a-gw-ngs-method-and-dedicated-bioinformatics-analysis
#4
Alexandra Bogožalec Košir, Alfred J Arulandhu, Marleen M Voorhuijzen, Hongmei Xiao, Rico Hagelaar, Martijn Staats, Adalberto Costessi, Jana Žel, Esther J Kok, Jeroen P van Dijk
The majority of feed products in industrialised countries contains materials derived from genetically modified organisms (GMOs). In parallel, the number of reports of unauthorised GMOs (UGMOs) is gradually increasing. There is a lack of specific detection methods for UGMOs, due to the absence of detailed sequence information and reference materials. In this research, an adapted genome walking approach was developed, called ALF: Amplification of Linearly-enriched Fragments. Coupling of ALF to NGS aims for simultaneous detection and identification of all GMOs, including UGMOs, in one sample, in a single analysis...
October 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29034627/-detection-of-transgenic-components-in-animal-feeds-on-shanxi-markets
#5
Jianqin Yuan, Hong Chang, Jianghe Zhao, Zhongwei Tang, Zongyong Shi, Jundong Wang
To assess the presence of genetically modified (GM) maize and soybean in a range of commercialized feed in Shanxi province of China in 2015, improved hexadecyltrimethy ammonium bromide (CTAB) method was used to extract DNA. The screening of packed feeds was carried out by qualitative PCR. Then positive feeds were unpacked and detected by the CaMV 35S promoter, NOS terminator, zSSIIb, Lectin and CryIA (b) genes. The identified maize and soybean events were confirmed by event-specific MON810 and GTS40-3-2. Results showed that 83...
November 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29026979/hpaec-pad-for-oligosaccharide-analysis-novel-insights-into-analyte-sensitivity-and-response-stability
#6
Matthias Mechelke, Jonathan Herlet, J Philipp Benz, Wolfgang H Schwarz, Vladimir V Zverlov, Wolfgang Liebl, Petra Kornberger
The rising importance of accurately detecting oligosaccharides in biomass hydrolyzates or as ingredients in food, such as in beverages and infant milk products, demands for the availability of tools to sensitively analyze the broad range of available oligosaccharides. Over the last decades, HPAEC-PAD has been developed into one of the major technologies for this task and represents a popular alternative to state-of-the-art LC-MS oligosaccharide analysis. This work presents the first comprehensive study which gives an overview of the separation of 38 analytes as well as enzymatic hydrolyzates of six different polysaccharides focusing on oligosaccharides...
October 12, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28993215/surveying-selected-european-feed-and-livestock-production-chains-for-features-enabling-the-case-specific-post-market-monitoring-of-livestock-for-intake-and-potential-health-impacts-of-animal-feeds-derived-from-genetically-modified-crops
#7
Gijs Kleter, Sarah McFarland, Alex Bach, Umberto Bernabucci, Paul Bikker, Luca Busani, Esther Kok, Kaloyan Kostov, Anna Nadal, Maria Pla, Bruno Ronchi, Marta Terre, Ralf Einspanier
This review, which has been prepared within the frame of the European Union (EU)-funded project MARLON, surveys the organisation and characteristics of specific livestock and feed production chains (conventional, organic, GM-free) within the EU, with an emphasis on controls, regulations, traceability, and common production practices. Furthermore, an overview of the origin of animal feed used in the EU as well as an examination of the use of genetically modified organisms (GMOs) in feed is provided. From the data, it shows that livestock is traceable at the herd or individual level, depending on the species...
October 6, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28953174/validity-of-the-general-movement-optimality-list-in-infants-born-preterm
#8
Tordis Ustad, Kari Anne I Evensen, Natascia Bertoncelli, Rossella Frassoldati, Fabrizio Ferrari
PURPOSE: To examine concurrent and predictive validity of the optimality list "Detailed Assessment of General Movements (GMs) During Preterm and Term Age." METHODS: Video clips of general movements were analyzed for 20 infants born preterm without severe brain lesions. Concurrent validity of the optimality list compared with the General Movement Assessment (GMA) was examined. The General Movement Optimality Scores (GMOSs) between infants with normal and poor repertoire GMA were examined...
October 2017: Pediatric Physical Therapy
https://www.readbyqxmd.com/read/28916034/colorimetric-biosensor-based-on-a-dnazyme-primer-and-its-application-in-logic-gate-operations-for-dna-screening
#9
Chenguang Wang, Nan Cheng, Longjiao Zhu, Yuancong Xu, Kunlun Huang, Pengyu Zhu, Shuifang Zhu, Wei Fu, Wentao Xu
A colorimetric biosensor for DNA screening was designed based on the conformational changes of the horseradish peroxidase (HRP)-mimicking DNAzyme. The scheme of DNA biosensing was designed based on the base pairing of DNAzyme sequence to inhibit the formation of HRP-mimicking hemin/G-quadruplex structures in the process of amplification. DNA could be amplified via the universal primer multiplex polymerase chain reaction (UP-M-PCR) and innovatively detected as color disappear in the reaction visible to the naked eye...
September 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28913806/endogenous-reference-genes-and-their-quantitative-real-time-pcr-assays-for-genetically-modified-bread-wheat-triticum-aestivum-l-detection
#10
Litao Yang, Sheng Quan, Dabing Zhang
Endogenous reference genes (ERG) and their derivate analytical methods are standard requirements for analysis of genetically modified organisms (GMOs). Development and validation of suitable ERGs is the primary step for establishing assays that monitoring the genetically modified (GM) contents in food/feed samples. Herein, we give a review of the ERGs currently used for GM wheat analysis, such as ACC1, PKABA1, ALMT1, and Waxy-D1, as well as their performances in GM wheat analysis. Also, we discussed one model for developing and validating one ideal RG for one plant species based on our previous research work...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28859885/case-studies-on-genetically-modified-organisms-gmos-potential-risk-scenarios-and-associated-health-indicators
#11
Barbara De Santis, Norbert Stockhofe, Jean-Michel Wal, Eefke Weesendorp, Jean Paul Lallès, Jeroen van Dijk, Esther Kok, Marzia De Giacomo, Ralf Einspanier, Roberta Onori, Carlo Brera, Paul Bikker, Jan van der Meulen, Kleter Gijs
Within the frame of the EU-funded MARLON project, background data were reviewed to explore the possibility of measuring health indicators during post-market monitoring for potential effects of feeds, particularly genetically modified (GM) feeds, on livestock animal health, if applicable. Four case studies (CSs) of potential health effects on livestock were framed and the current knowledge of a possible effect of GM feed was reviewed. Concerning allergenicity (CS-1), there are no case-reports of allergic reactions or immunotoxic effects resulting from GM feed consumption as compared with non-GM feed...
August 28, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28844131/one-novel-multiple-target-plasmid-reference-molecule-targeting-eight-genetically-modified-canola-events-for-genetically-modified-canola-detection
#12
Zhuqing Li, Xiang Li, Canhua Wang, Guiwen Song, Liqun Pi, Lan Zheng, Dabing Zhang, Litao Yang
Multiple-target plasmid DNA reference materials have been generated and utilized as good substitutes of matrix-based reference materials in the analysis of genetically modified organisms (GMOs). Herein, we report the construction of one multiple-target plasmid reference molecule, pCAN, which harbors eight GM canola event-specific sequences (RF1, RF2, MS1, MS8, Topas 19/2, Oxy235, RT73, and T45) and a partial sequence of the canola endogenous reference gene PEP. The applicability of this plasmid reference material in qualitative and quantitative PCR assays of the eight GM canola events was evaluated, including the analysis of specificity, limit of detection (LOD), limit of quantification (LOQ), and performance of pCAN in the analysis of various canola samples, etc...
September 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28821537/investigating-novice-and-expert-conceptions-of-genetically-modified-organisms
#13
Lisa M Potter, Sarah A Bissonnette, Jonathan D Knight, Kimberly D Tanner
The aspiration of biology education is to give students tools to apply knowledge learned in the classroom to everyday life. Genetic modification is a real-world biological concept that relies on an in-depth understanding of the molecular behavior of DNA and proteins. This study investigated undergraduate biology students' conceptions of genetically modified organisms (GMOs) when probed with real-world, molecular and cellular, and essentialist cues, and how those conceptions compared across biology expertise...
2017: CBE Life Sciences Education
https://www.readbyqxmd.com/read/28819142/development-and-inter-laboratory-assessment-of-droplet-digital-pcr-assays-for-multiplex-quantification-of-15-genetically-modified-soybean-lines
#14
Alexandra Bogožalec Košir, Bjørn Spilsberg, Arne Holst-Jensen, Jana Žel, David Dobnik
Quantification of genetically modified organisms (GMOs) in food and feed products is often required for their labelling or for tolerance thresholds. Standard-curve-based simplex quantitative polymerase chain reaction (qPCR) is the prevailing technology, which is often combined with screening analysis. With the rapidly growing number of GMOs on the world market, qPCR analysis becomes laborious and expensive. Innovative cost-effective approaches are therefore urgently needed. Here, we report the development and inter-laboratory assessment of multiplex assays to quantify GMO soybean using droplet digital PCR (ddPCR)...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28795123/corrigendum-to-data-in-support-of-the-detection-of-genetically-modified-organisms-gmos-in-food-and-feed-samples-data-brief-7-2016-pp-243-252
#15
(no author information available yet)
[This corrects the article DOI: 10.1016/j.dib.2016.02.035.].
September 2016: Data in Brief
https://www.readbyqxmd.com/read/28680789/is-it-only-the-regulatory-status-broadening-the-debate-on-cisgenic-plants
#16
Lilian van Hove, Frøydis Gillund
In current debates on emerging technologies for plant breeding in Europe, much attention has been given to the regulatory status of these techniques and their public acceptance. At present, both genetically modified plants with cisgenic approaches-using genes from crossable species-as well as transgenic approaches-using genes from different species-fall under GMO regulation in the EU and both are mandatorily labelled as GMOs. Researchers involved in the early development of cisgenic GM plants convey the message that the potential use and acceptance of cisgenic approaches will be seriously hindered if GMO regulations are not adjusted...
2017: Environmental Sciences Europe
https://www.readbyqxmd.com/read/28664693/readiness-of-adolescents-to-use-genetically-modified-organisms-according-to-their-knowledge-and-emotional-attitude-towards-gmos
#17
Stanisław Lachowski, Anna Jurkiewicz, Piotr Choina, Magdalena Florek-Łuszczki, Agnieszka Buczaj, Małgorzata Goździewska
INTRODUCTION: Agriculture based on genetically modified organisms plays an increasingly important role in feeding the world population, which is evidenced by a considerable growth in the size of land under genetically modified crops (GM). Uncertainty and controversy around GM products are mainly due to the lack of accurate and reliable information, and lack of knowledge concerning the essence of genetic modifications, and the effect of GM food on the human organism, and consequently, a negative emotional attitude towards what is unknown...
June 7, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28647548/the-bioeconomy-in-poland-within-the-context-of-the-european-union
#18
REVIEW
Ewa Woźniak, Tomasz Twardowski
This article outlines the potential of the development of bioeconomy in Poland in the context of the European Union (EU). The analyses take into account the concept of bioeconomy, the overview of documents referring to the bioeconomy in the EU, including policy frameworks and agendas. Many countries including Poland emphasise the importance of bioeconomy, but have not yet developed a complex strategy. The state of bioeconomy in Poland is characterised by the sectors of agriculture, forestry and food production, as well as parts of the chemical, biotechnology and energy industries...
June 21, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28645870/impact-on-environment-ecosystem-diversity-and-health-from-culturing-and-using-gmos-as-feed-and-food
#19
REVIEW
Aristidis M Tsatsakis, Muhammad Amjad Nawaz, Victor A Tutelyan, Kirill S Golokhvast, Olga-Ioanna Kalantzi, Duck Hwa Chung, Sung Jo Kang, Michael D Coleman, Nadia Tyshko, Seung Hwan Yang, Gyuhwa Chung
Modern agriculture provides the potential for sustainable feeding of the world's increasing population. Up to the present moment, genetically modified (GM) products have enabled increased yields and reduced pesticide usage. Nevertheless, GM products are controversial amongst policy makers, scientists and the consumers, regarding their possible environmental, ecological, and health risks. Scientific-and-political debates can even influence legislation and prospective risk assessment procedure. Currently, the scientifically-assessed direct hazardous impacts of GM food and feed on fauna and flora are conflicting; indeed, a review of literature available data provides some evidence of GM environmental and health risks...
September 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28596811/genetic-engineering-and-human-mental-ecology-interlocking-effects-and-educational-considerations
#20
Ramsey Affifi
This paper describes some likely semiotic consequences of genetic engineering on what Gregory Bateson has called "the mental ecology" (1979) of future humans, consequences that are less often raised in discussions surrounding the safety of GMOs (genetically modified organisms). The effects are as follows: an increased 1) habituation to the presence of GMOs in the environment, 2) normalization of empirically false assumptions grounding genetic reductionism, 3) acceptance that humans are capable and entitled to decide what constitutes an evolutionary improvement for a species, 4) perception that the main source of creativity and problem solving in the biosphere is anthropogenic...
2017: Biosemiotics
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