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https://www.readbyqxmd.com/read/29746092/metabolic-recruitment-and-directed-evolution-of-nucleoside-triphosphate-uptake-in-escherichia-coli
#1
Valerie Pezo, Camille Hassan, Dominique Louis, Bruno Sargueil, Piet Herdewijn, Philippe Marliere
We report the design and elaboration of a selection protocol for importing a canonical substrate of DNA polymerase, thymidine triphosphate (dTTP) in Escherichia coli. Bacterial strains whose growth depend on dTTP uptake, through the action of an algal plastid transporter expressed from a synthetic gene inserted in the chromosome, were constructed and shown to withstand the simultaneous loss of thymidylate synthase and thymidine kinase. Such thyA tdk dual deletant strains provide an experimental model of tight nutritional containment for preventing dissemination of microbial GMOs...
May 10, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29717438/multiplex-droplet-digital-pcr-protocols-for-quantification-of-gm-maize-events
#2
David Dobnik, Bjørn Spilsberg, Alexandra Bogožalec Košir, Dejan Štebih, Dany Morisset, Arne Holst-Jensen, Jana Žel
The standard-curve based simplex quantitative polymerase chain reaction (qPCR) has been the gold standard for DNA target quantification for more than a decade. The large and growing number of individual analyses needed to test for genetically modified organisms (GMOs) is reducing the cost-effectiveness of qPCR. Droplet digital PCR (ddPCR) enables absolute quantification without standard curves, avoids the amplification efficiency bias observed with qPCR, allows more accurate estimations at low target copy numbers and, in combination with multiplexing, significantly improves cost efficiency...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29680759/impact-of-genetically-modified-organisms-on-aquatic-environments-review-of-available-data-for-the-risk-assessment
#3
REVIEW
Antonia Pott, Mathias Otto, Ralf Schulz
The aquatic environment is strongly connected to the surrounding agricultural landscapes, which regularly serve as sources of stressors such as agrochemicals. Genetically modified crops, which are cultivated on a large scale in many countries, may also act as stressors. Despite the commercial use of genetically modified organisms (GMOs) for over 20years, their impact on the aquatic environment came into focus only 10years ago. We present the status quo of the available scientific data in order to provide an input for informed aquatic risk assessment of GMOs...
April 19, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29675412/capacities-for-the-risk-assessment-of-gmos-challenges-to-build-sustainable-systems
#4
Danilo Fernández Ríos, Clara Rubinstein, Carmen Vicién
The need for functional risk assessment bodies in general, and in the biosafety field in particular, demands continued efforts and commitment from regulatory agencies, if results that are sustainable in time are to be achieved. The lack of formal processes that ensure continuity in the application of state of the art scientific criteria, the high rotation in some cases or the lack of experienced professionals, in others, is a challenge to be addressed. Capacity building initiatives with different approaches and degrees of success have been implemented in many countries over the years, supported by diverse governmental and non-governmental organizations...
2018: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29652133/ultra-sensitive-single-fluorescence-labeled-probe-mediated-sup-m-ddpcr-for-high-throughput-gmo-screening
#5
Chenqi Niu, Yuancong Xu, Chao Zhang, Pengyu Zhu, Kunlun Huang, Yunbo Luo, Wentao Xu
As genetically modified (GM) technology develops and genetically modified organisms (GMOs) become more available, GMOs face increasing regulations and pressure to adhere to strict labeling guidelines. A singleplex detection method cannot perform the high-throughput analysis necessary for optimal GMO detection. Combining the advantages of multiplex detection and droplet digital polymerase chain reaction (ddPCR), a single universal primer-multiplex-ddPCR (SUP-M-ddPCR) strategy was proposed for accurate broad-spectrum screening and quantification...
April 13, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29609161/electrochemical-genoassays-on-gold-coated-magnetic-nanoparticles-to-quantify-genetically-modified-organisms-gmos-in-food-and-feed-as-gmo-percentage
#6
Alexandra Plácido, Clara Pereira, Alexandra Guedes, M Fátima Barroso, Rebeca Miranda-Castro, Noemí de-Los-Santos-Álvarez, Cristina Delerue-Matos
The integration of nanomaterials in the field of (bio)sensors has allowed developing strategies with improved analytical performance. In this work, ultrasmall core-shell Fe3 O4 @Au magnetic nanoparticles (MNPs) were used as the platform for the immobilization of event-specific Roundup Ready (RR) soybean and taxon-specific DNA sequences. Firstly, monodisperse Fe3 O4 MNPs were synthesized by thermal decomposition and subsequently coated with a gold shell through reduction of Au(III) precursor on the surface of the MNPs in the presence of an organic capping agent...
March 21, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29594619/colorimetric-detection-of-genetically-modified-organisms-based-on-exonuclease-iii-assisted-target-recycling-and-hemin-g-quadruplex-dnazyme-amplification
#7
Decai Zhang, Weijia Wang, Qian Dong, Yunxiu Huang, Dongmei Wen, Yuejing Mu, Yong Yuan
An isothermal colorimetric method is described for amplified detection of the CaMV 35S promoter sequence in genetically modified organism (GMO). It is based on (a) target DNA-triggered unlabeled molecular beacon (UMB) termini binding, and (b) exonuclease III (Exo III)-assisted target recycling, and (c) hemin/G-quadruplex (DNAzyme) based signal amplification. The specific binding of target to the G-quadruplex sequence-locked UMB triggers the digestion of Exo III. This, in turn, releases an active G-quadruplex segment and target DNA for successive hybridization and cleavage...
December 21, 2017: Mikrochimica Acta
https://www.readbyqxmd.com/read/29584422/development-of-certified-matrix-based-reference-material-as-calibrator-for-genetically-modified-rice-g6h1-analysis
#8
Yu Yang, Liang Li, Hui Yang, Xiaying Li, Xiujie Zhang, Junfeng Xu, Dabing Zhang, Wujun Jin, Litao Yang
The accurate monitoring and quantification of genetically modified organisms (GMOs) are key points for the implementation of labelling regulations, and a certified reference material (CRM) acts as the scaleplate for quantifying the GM contents of foods/feeds and evaluating a GMO analytical method or equipment. Herein, we developed a series of CRMs for transgenic rice event G6H1, which possesses insect-resistant and herbicide-tolerant traits. Three G6H1 CRMs were produced by mixing seed powders obtained from homozygous G6H1 and its recipient cultivar Xiushui 110 at mass ratios of 49...
March 27, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29574561/critical-assessment-of-digital-pcr-for-the-detection-and-quantification-of-genetically-modified-organisms
#9
REVIEW
Tigst Demeke, David Dobnik
The number of genetically modified organisms (GMOs) on the market is steadily increasing. Because of regulation of cultivation and trade of GMOs in several countries, there is pressure for their accurate detection and quantification. Today, DNA-based approaches are more popular for this purpose than protein-based methods, and real-time quantitative PCR (qPCR) is still the gold standard in GMO analytics. However, digital PCR (dPCR) offers several advantages over qPCR, making this new technique appealing also for GMO analysis...
March 24, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29565638/biosensors-for-gmo-testing-nearly-25-years-of-research
#10
Marta Sánchez-Paniagua López, Carmen Lorena Manzanares-Palenzuela, Beatriz López-Ruiz
In the nearly two decades since genetically modified organisms (GMOs) were first commercialized, genetically engineered crops have gained ground on their conventional counterparts, reaching 185 million hectares worldwide in 2016. The technology has bestowed most of its benefits on enhancing crop productivity with two main traits currently dominating the market: insect-resistant and herbicide-tolerant crops. Despite their rapid and vast adoption by farmers worldwide, GMOs have generated heated debates, especially in European countries (EU), driven mostly by consumers concerned about safety of transgenic foods and about the potential impact on the environment...
March 22, 2018: Critical Reviews in Analytical Chemistry
https://www.readbyqxmd.com/read/29561212/sowing-the-seeds-of-skepticism-russian-state-news-and-anti-gmo-sentiment
#11
Shawn F Dorius, Carolyn J Lawrence-Dill
Biotech news coverage in English-language Russian media fits the profile of the Russian information warfare strategy described in recent military reports. This raises the question of whether Russia views the dissemination of anti-GMO information as just one of many divisive issues it can exploit as part of its information war, or if GMOs serve more expansive disruptive purposes. Distinctive patterns in Russian news provide evidence of a coordinated information campaign that could turn public opinion against genetic engineering...
March 21, 2018: GM Crops & Food
https://www.readbyqxmd.com/read/29521202/biotechnological-production-of-pharmaceuticals-and-biopharmaceuticals-in-plant-cell-and-organ-cultures
#12
Diego Hidalgo, Raul Sanchez, Liliana Lalaleo, Mercedes Bonfill, Purificacion Corchete, Javier Palazon
BACKGROUND: Plant biofactories are biotechnological platforms based on plant cell and organ cultures used for the production of pharmaceuticals and biopharmaceuticals, although to date only a few of these systems have successfully been implemented at an industrial level. Metabolic engineering is possibly the most straightforward strategy to boost pharmaceutical production in plant biofactories, but social opposition to the use of GMOs means empirical approaches are still being used. Plant secondary metabolism involves thousands of different enzymes, some of which catalyze specific reactions, giving one product from a particular substrate, whereas others can yield multiple products from the same substrate...
March 9, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29517296/a-pot-of-gold-at-the-end-of-the-rainbow-a-spectrum-of-attitudes-to-assisted-reproductive-technologies-in-ireland
#13
Maria Dempsey, Rob King, Andrea Nagy
OBJECTIVE: New technologies present new ethical dilemmas. Our ethical intuitions may mislead us in relation to new technologies such as nuclear power, vaccines, GMOs and assistive reproductive technologies (ART). Between 1999 and 2008 the number of ART treatment cycles increased by 265% in Ireland. The implications and potentials of such technologies are profound - challenging existing understanding of humans' relationships to reproduction. Because such technologies are comparatively unregulated, and their use has only been occurring for a single generation, detailed investigation of how awareness of ART influences understanding of personal fertility is needed...
February 2018: Journal of Reproductive and Infant Psychology
https://www.readbyqxmd.com/read/29509719/gene-editing-of-microalgae-scientific-progress-and-regulatory-challenges-in-europe
#14
REVIEW
Andrew Spicer, Attila Molnar
It is abundantly clear that the development of gene editing technologies, represents a potentially powerful force for good with regard to human and animal health and addressing the challenges we continue to face in a growing global population. This now includes the development of approaches to modify microalgal strains for potential improvements in productivity, robustness, harvestability, processability, nutritional composition, and application. The rapid emergence and ongoing developments in this area demand a timely review and revision of the current definitions and regulations around genetically modified organisms (GMOs), particularly within Europe...
March 6, 2018: Biology
https://www.readbyqxmd.com/read/29337228/withdrawn-monitoring-the-prevalence-of-genetically-modified-gm-maize-in-iran-food-products
#15
Motahreh Sadat Hosseini, Gilda Eslami, Bahador Hajimohammadi, Hossein Fallahzadeh, Zahra Derakhshan, Gea Oliveri Conti, Margherita Ferrante
This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal.
January 11, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29321648/comparative-analysis-of-mirna-expression-profiles-in-transgenic-and-non-transgenic-rice-using-mirna-seq
#16
Cheng Peng, Xiaoyun Chen, Xiaofu Wang, Xiaoli Xu, Wei Wei, Congmao Wang, Junfeng Xu
Safety assessment for genetically modified organisms (GMOs) is required before their release. To date, miRNAs that play important roles in eukaryotic gene regulation have not been considered in the current assessment system. In this study, we identified 6 independent Bt and EPSPS GM rice lines using PCR and immune strip. We analyzed the expression levels of Cry1Ac and EPSPS using quantitative real-time PCR and western blot. Further, miRNAs from the developing seeds of the 6 GM rice lines and the wild-type line were investigated using deep sequencing and bioinformatic approaches...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29236423/gmos-genetically-modified-organisms-or-genuinely-mixed-opinions-a-reasonable-consumer%C3%A2-s-understanding-of-the-terms-gmo-and-non-gmo-and-the-struggle-to-set-a-standard-%C3%AF-%C2%AA
#17
https://www.readbyqxmd.com/read/29173000/the-central-dogma-gmo-and-defective-epistemology
#18
Giovanni Tagliabue
The expression "Genetically Modified Organisms" was coined to indicate a group of agricultural products (mostly crops and vegetables), modified through direct DNA recombination in order to obtain useful phenotypic traits or to inhibit undesirable characteristics. But the border between rDNA ("GMO") and other biotech methods is blurred. Moreover, the ill-assorted group is frequently charged with having peculiar, negative characteristics: many activists, part of the public and a few social science scholars think that "GMOs" are all dubious, even inherently dangerous...
October 2, 2017: GM Crops & Food
https://www.readbyqxmd.com/read/29150519/biological-containment-of-genetically-modified-bacillus-subtilis
#19
Siamand Hosseini, Alex Curilovs, Simon M Cutting
Genetic manipulation of bacterial spores of the genus Bacillus has shown potential for vaccination and for delivery of drugs or enzymes. Remarkably, proteins displayed on the spore surface retain activity and generally are not degraded. The heat stability of spores coupled with their desiccation resistance makes them suitable for delivery to humans or to animals by the oral route. Despite these attributes one regulatory obstacle has remained regarding the fate of recombinant spores shed into the environment as viable spores...
November 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29147107/debated-agronomy-public-discourse-and-the-future-of-biotechnology-policy-in-ghana
#20
Joseph A Braimah, Kilian N Atuoye, Siera Vercillo, Carrie Warring, Isaac Luginaah
This paper examines the highly contested and ongoing biotechnology (Bt) policy-making process in Ghana. We analyse media content on how Bt is viewed in the context of Ghana's parliamentary debate on the Plant Breeders Bill and within the broader public policy-making literature. This paper does not seek to take a position on Bt or the Bill, but to understand how policy actors influence the debate with political and scientific rhetoric in Ghana. The study reveals that in the midst of scientific uncertainties of Bt's potential for sustainable agriculture production and food security, policy decisions that encourage its future adoption are heavily influenced by health, scientific, economic, environmental and political factors dictated by different ideologies, values and norms...
2017: Global Bioethics, Problemi di Bioetica
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