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Trabecular meshwork

H Salcedo, D Arciniega, M Mayorga, L Wu
PURPOSE: The water-drinking test (WDT) has recently re-emerged as a possible way to determine the competency of the trabecular meshwork. We performed a prospective interventional study to test the hypothesis that the WDT could be useful in assessing fluctuations in patients undergoing treatment for primary open angle glaucoma (POAG). METHODS: We included 122 patients; 62 on medical treatment for POAG (n=123 eyes) and 60 controls (n=120 eyes). The study group had been on intraocular pressures (IOP) lowering treatment continuously for at least 3months with stable IOP...
May 16, 2018: Journal Français D'ophtalmologie
Sheridan M Hoy
Latanoprostene bunod ophthalmic solution 0.024% (hereafter referred to as latanoprostene bunod 0.024%) [Vyzulta™] is a nitric oxide (NO)-donating prostaglandin F2α analogue approved in the USA for the reduction of intraocular pressure (IOP) in patients with open-angle glaucoma (OAG) or ocular hypertension. It is thought to lower IOP by increasing aqueous humour outflow through the uveoscleral pathway (mediated by latanoprost acid) and increasing the facility of aqueous humour outflow through the trabecular meshwork pathway (mediated by NO)...
May 14, 2018: Drugs
Miyuki Inoue-Mochita, Toshihiro Inoue, Sachi Kojima, Akiko Futakuchi, Tomokazu Fujimoto, Saori Sato-Ohira, Utako Tsutsumi, Hidenobu Tanihara
Glaucoma is one of the major causes of blindness, and TGF-β2 has been found to be elevated in the aqueous humor of eyes with primary open-angle glaucoma (POAG). TGF-β2 in aqueous humor causes the glaucoma-related fibrosis of human trabecular meshwork (HTM), suggesting an important role of TGF-β in POAG pathogenesis. Here, we sought to elucidate the effects of IL-6 trans-signaling on TGF-β signaling in HTM cells. Using a multiplex immunoassay, POAG patients decreased IL-6 levels and increased soluble IL-6 receptor (sIL-6R) levels compared with the controls...
May 11, 2018: Journal of Biological Chemistry
Ramesh B Kasetti, Prabhavathi Maddineni, Pinkal Patel, Charles Searby, Val C Sheffield, Gulab S Zode
Elevation of intraocular pressure (IOP) is a serious adverse effect of glucocorticoid (GC) therapy. Increased extracellular matrix (ECM) accumulation and endoplasmic reticulum (ER) stress in the trabecular meshwork (TM) is associated with GC-induced IOP elevation. However, the molecular mechanisms by which GCs induce ECM accumulation and ER stress in the TM have not been determined. Here, we show that a potent GC, dexamethasone (Dex), activates transforming growth factor β (TGFβ) signaling, leading to GC-induced ECM accumulation, ER stress and IOP elevation...
May 9, 2018: Journal of Biological Chemistry
Zhi-Peng You, Yue-Zhi Zhang, Yu-Lan Zhang, Lu Shi, Ke Shi
The aim of the present study was to investigate the effect of homocysteine (Hcy) in on human trabecular meshwork cells (HTMCs). A total of 41 patients with primary open-angle glaucoma (POAG) and 53 patients with senile cataracts (control group) were recruited. Plasma and aqueous humor samples were collected and the Hcy concentrations were determined using enzymatic cycling assays. In cell experiments, normal HTMCs were passaged and randomly divided into a blank control group, a normal HTMC group and experimental groups, which were treated with different concentrations of Hcy...
May 2018: Experimental and Therapeutic Medicine
Gianluca Scuderi, Maria Teresa Contestabile, Luca Scuderi, Aloisa Librando, Vito Fenicia, Siavash Rahimi
INTRODUCTION: Potential factors influencing stereopsis were investigated in patients with both refractive accommodative esotropia (RAE) and amblyopia. Pigment dispersion syndrome (PDS) is a condition where anomalous iridozonular contact leads to pigment dispersion throughout the anterior segment and the released pigment is abnormally deposited on various ocular structures. CLINICAL PRESENTATION: The clinical presentation of PDS is defined by the presence of pigmented cells on the corneal endothelium, an increase of pigmentation of the trabecular meshwork, and mid-periphery transillumination defects of the iris...
May 2, 2018: International Ophthalmology
Yalong Dang, Chao Wang, Priyal Shah, Susannah Waxman, Ralitsa T Loewen, Ying Hong, Hamed Esfandiari, Nils A Loewen
PURPOSE: To evaluate three different microincisional ab interno trabeculectomy procedures in a porcine eye perfusion model. METHODS: In perfused porcine anterior segments, 90° of trabecular meshwork (TM) was ablated using the Trabectome (T; n = 8), Goniotome (G; n = 8), or Kahook device (K; n = 8). After 24 h, additional 90° of TM was removed. Intraocular pressure (IOP) and outflow facility were measured at 5 and 10 μl/min perfusion to simulate an elevated IOP...
May 2, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
Yalong Dang, Susannah Waxman, Chao Wang, Priyal Shah, Ralitsa T Loewen, Nils A Loewen
Background: Outflow regulation and phagocytosis are key functions of the trabecular meshwork (TM), but it is not clear how the two are related in secondary open angle glaucomas characterized by an increased particle load. We hypothesized that diminished TM phagocytosis is not the primary cause of early ocular hypertension and recreated pigment dispersion in a porcine ex vivo model. Methods: Sixteen porcine anterior chamber cultures received a continuous infusion of pigment granules (Pg), while 16 additional anterior chambers served as controls (C)...
2018: F1000Research
Zhiqi Chen, Yinwei Song, Mu Li, Wei Chen, Shiliang Liu, Zhen Cai, Liugui Chen, Yan Xiang, Hong Zhang, Junming Wang
PURPOSE: Myopes have a higher risk for open-angle glaucoma; however, the reason is not clear. This study aims to explore the morphologic alterations of Schlemm's canal and trabecular meshwork size in highly myopic eyes. METHODS: Forty myopic eyes from 40 patients with high myopia and 40 control eyes from 40 subjects with low myopia, emmetropia or low hyperopia were enrolled. Optical coherence tomography (OCT) was used to image Schlemm's canal and trabecular meshwork size...
May 2018: Ophthalmic & Physiological Optics: the Journal of the British College of Ophthalmic Opticians (Optometrists)
Leona T Y Ho, Nikolai Skiba, Christoph Ullmer, Ponugoti Vasantha Rao
Purpose: Lysophosphatidic acid (LPA), a bioactive lipid, has been shown to increase resistance to aqueous humor outflow (AH) through the trabecular meshwork (TM). The molecular basis for this response of the TM to LPA, however, is not completely understood. In this study, we explored the possible involvement of mechanosensitive Yes-associated protein (YAP) and its paralog, transcriptional coactivator with PDZ-binding domain (TAZ), transcriptional activation in extracellular matrix (ECM) production by LPA-induced contractile activity in human TM cells (HTM)...
April 1, 2018: Investigative Ophthalmology & Visual Science
Reiko Yamagishi-Kimura, Megumi Honjo, Takashi Komizo, Takashi Ono, Akiko Yagi, Jinhee Lee, Kazunori Miyata, Tomokazu Fujimoto, Toshihiro Inoue, Hidenobu Tanihara, Junko Nishida, Takatoshi Uchida, Yuka Araki, Makoto Aihara
Purpose: To explore interactions between pilocarpine and the ROCK inhibitor, ripasudil, on IOP and pupil diameter in human eyes, and morphological and functional changes in outflow tissues in vitro. Methods: IOP and pupil diameter were measured after pilocarpine and/or ripasudil, which were topically applied in healthy subjects. Human trabecular meshwork (HTM) cells were used in a gel contraction assay, for the evaluation of phosphorylation of myosin light chain and cofilin, and immunostaining for cytoskeletal proteins...
April 1, 2018: Investigative Ophthalmology & Visual Science
Francesco Impagnatiello, Elena Bastia, Nicoletta Almirante, Stefania Brambilla, Brigitte Duquesroix, Angela C Kothe, Michael V W Bergamini
In patients with ocular hypertension or glaucoma all treatments aim at lowering intraocular pressure (IOP) by modulating aqueous humor (AH) production and/or uveoscleral and trabecular meshwork /Schlemm's canal AH drainage. Prostaglandin (PG) analogues are considered the 'gold standard', and are the most frequently used IOP-lowering agents. Recent data support an important role of nitric oxide (NO) in regulating IOP. Thus novel PG analogues carrying a NO-donating moiety were recently advanced. Latanoprostene bunod (LBN) and NCX 470, respectively a NO-donating derivative of latanoprost and bimatoprost, are examples of such compounds...
April 18, 2018: British Journal of Pharmacology
Sunee Chansangpetch, Sasiprapa Prombhul, Visanee Tantisevi, Pimpayao Sodsai, Anita Manassakorn, Nattiya Hirankarn, Shan C Lin
Background/Aims: Epigenetic mechanisms via DNA methylation may be related to glaucoma pathogenesis. This study aimed to determine the global DNA methylation level of the trabeculectomy specimens among patients with different types of glaucoma and normal subjects. Methods: Trabeculectomy sections from 16 primary open-angle glaucoma (POAG), 12 primary angle-closure glaucoma (PACG), 16 secondary glaucoma patients, and 10 normal controls were assessed for DNA methylation using combined-bisulfite restriction analysis...
2018: Journal of Ophthalmology
Andreas Katsanos, Anastasios G Konstas, Dimitrios G Mikropoulos, Luciano Quaranta, Irini C Voudouragkaki, Georgios P Athanasopoulos, Ioannis Asproudis, Miguel A Teus
In the last decade, selective laser trabeculoplasty (SLT) has been commonly used in the management of several different types of glaucoma, as either primary or adjunct therapy. The technique has an excellent safety profile and is at least as effective as argon laser trabeculoplasty. Although the actual mechanism of action of SLT remains unclear, evidence has shown that it does not induce morphologically evident trabecular meshwork alterations. SLT's non-disruptive mode of action offers the advantage of repeatability...
April 11, 2018: Advances in Therapy
Ke Wang, Guorong Li, A Thomas Read, Iris Navarro, Ashim K Mitra, W Daniel Stamer, Todd Sulchek, C Ross Ethier
It has been suggested that common mechanisms may underlie the pathogenesis of primary open-angle glaucoma (POAG) and steroid-induced glaucoma (SIG). The biomechanical properties (stiffness) of the trabecular meshwork (TM) have been shown to differ between POAG patients and unaffected individuals. While features such as ocular hypertension and increased outflow resistance in POAG and SIG have been replicated in mouse models, whether changes of TM stiffness contributes to altered IOP homeostasis remains unknown...
April 11, 2018: Scientific Reports
Hannah C Webber, Jaclyn Y Bermudez, J Cameron Millar, Weiming Mao, Abbot F Clark
Purpose: Wnt/β-catenin signaling in the trabecular meshwork (TM) is required for maintaining normal intraocular pressure (IOP), although the mechanism(s) behind this are unknown. We hypothesize that Wnt/β-catenin signaling regulates IOP via β-catenin's effects on cadherin junctions. Methods: Nonglaucomatous primary human TM (NTM) cells were treated with or without 100 ng/ml Wnt3a, 1 μg/ml sFRP1, or both for 4 to 48 hours. Cells were immunostained for β-catenin, total cadherins, or cadherin isoforms...
March 1, 2018: Investigative Ophthalmology & Visual Science
Yuwen Wang, Heding Zhou, Xiaotian Liu, Yin Han, Suqi Pan, Yanyan Wang
BACKGROUND: The trabecular meshwork (TM) plays a critical role in the outflow of aqueous humor. OBJECTIVES: In this study, we aimed to investigate the effect of miR-181a on H2O2-induced apoptosis in TM cells. MATERIAL AND METHODS: Human primary explant-derived TM cells were cultured in fibroblast medium and then treated with different concentrations of H2O2 for 2 h. We used a series of methods to carry out the research, such as MTT assay, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), apoptosis assay, and western blot methodology...
March 26, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Yalong Dang, Susannah Waxman, Chao Wang, Ralitsa T Loewen, Ming Sun, Nils A Loewen
Pigment dispersion can lead to pigmentary glaucoma, a poorly understood condition of younger myopic eyes with fluctuating high intraocular pressure. It has been difficult to investigate its pathogenesis without a model similar to human eyes in size and behavior. Here we present a porcine ex vivo model that recreates several features of pigmentary glaucoma, including intraocular hypertension, accumulation of pigment in the trabecular meshwork, and declining phagocytosis. We found that trabecular meshwork cells regulate outflow, form actin stress fibers, and have a decreased phagocytic activity...
April 3, 2018: Scientific Reports
Ganesh Prasanna, Luciana Ferrara, Christopher Adams, Takeru Ehara, Byron Li, Louis Yang, Chuanxi Xiang, Christopher Thow Hing Ng, Sean Kim, Christopher Towler, Todd Topley, Cale McAllister, Malay Ghosh, Ronald Newton, Rebecca Stacy, Dennis S Rice, Muneto Mogi
Purpose: The nitric oxide/soluble guanylate cyclase/protein kinase G (NO/sGC/PKG) is known to be involved in the regulation of intraocular pressure (IOP) and may be dysregulated in glaucoma. The purpose is to demonstrate that the sGC activator MGV354 lowers IOP in a monkey model of glaucoma and could be considered as a possible new clinical drug candidate. Methods: Changes to cGMP were assessed in primary human trabecular meshwork (hNTM) cells and binding studies were conducted using human sGC full-length protein...
April 1, 2018: Investigative Ophthalmology & Visual Science
Alessandro Mauro, Nicola Massarotti, Mohamed Salahudeen, Mario R Romano, Vito Romano, Perumal Nithiarasu
The present work describes the application of the generalised porous medium model to study heat and fluid flow in healthy and glaucomatous eyes of different subject specimens, considering the presence of ocular cavities and porous tissues. The 2D computational model, implemented into the open-source software OpenFOAM, has been verified against benchmark data for mixed convection in domains partially filled with a porous medium. The verified model has been employed to simulate the thermo-fluid dynamic phenomena occurring in the anterior section of four patient-specific human eyes, considering the presence of anterior chamber (AC), trabecular meshwork (TM), Schlemm's canal (SC), and collector channels (CC)...
March 22, 2018: Medical & Biological Engineering & Computing
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