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Mir-21 pdcd4

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https://www.readbyqxmd.com/read/27922982/mir-21-is-overexpressed-in-hydatidiform-mole-tissues-and-promotes-proliferation-migration-and-invasion-in-choriocarcinoma-cells
#1
Ya-Xin Wang, Jiu-Ru Zhao, Yue-Ying Xu, Wei-Bin Wu, Hui-Juan Zhang
OBJECTIVE: The aims of this study were to make clear whether miR-21 was dysregulated in hydatidiform mole (HM) tissues and choriocarcinoma (CCA) cells, to elucidate whether aberrant miR-21 expression would affect the function of CCA cells, and to find out whether there was a relationship between miR-21 and AKT, PDCD4, and PTEN in CCA cells. METHODS: Fresh and formalin-fixed, paraffin-embedded trophoblastic tissues (normal first trimester placentas and HMs) were retrieved from the biobank in the International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University...
December 3, 2016: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/27876813/oncogenic-transformation-of-human-lung-bronchial-epithelial-cells-induced-by-arsenic-involves-ros-dependent-activation-of-stat3-mir-21-pdcd4-mechanism
#2
Poyil Pratheeshkumar, Young-Ok Son, Sasidharan Padmaja Divya, Lei Wang, Zhuo Zhang, Xianglin Shi
Arsenic is a well-documented human carcinogen. The present study explored the role of the onco-miR, miR-21 and its target protein, programmed cell death 4 (PDCD4) in arsenic induced malignant cell transformation and tumorigenesis. Our results showed that treatment of human bronchial epithelial (BEAS-2B) cells with arsenic induces ROS through p47(phox), one of the NOX subunits that is the key source of arsenic-induced ROS. Arsenic exposure induced an upregulation of miR-21 expression associated with inhibition of PDCD4, and caused malignant cell transformation and tumorigenesis of BEAS-2B cells...
November 23, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27832626/ischemic-postconditioning-regulated-mir-499-protects-the-rat-heart-against-ischemia-reperfusion-injury-by-inhibiting-apoptosis-through-pdcd4
#3
Jianbing Zhu, Kang Yao, Qian Wang, Junjie Guo, Hongtao Shi, Leilei Ma, Haibo Liu, Wei Gao, Yunzeng Zou, Junbo Ge
BACKGROUND: Here, we determined miR-499 involvement in the protective effect of ischemic postconditioning (IPC) against myocardial ischemia/reperfusion (I/R) injury and identified the underlying mechanisms. METHODS: To investigate the cardioprotective effect of IPC-induced miR-499, rats were divided into the following five groups: sham, I/R, IPC, IPC + scramble, and IPC + antagomiR-499. Hemodynamic indexes were measured by carotid-artery intubation to assess left ventricular function ...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27824649/downregulation-of-microrna-21-in-colonic-cd3-t-cells-in-uc-remission
#4
Yugo Ando, Luca Mazzurana, Marianne Forkel, Kazuichi Okazaki, Mamiko Aoi, Peter T Schmidt, Jenny Mjösberg, Francesca Bresso
BACKGROUND: In active inflammatory bowel disease (IBD), microRNA expression profiling consistently features disease-specific signatures, and microRNA-21 (miR-21) has been shown to be upregulated in the inflamed colon of patients with active ulcerative colitis (UC). However, the cellular sources of miR-21 expression in IBD tissues have not yet been identified. We sought to determine the expression levels of miR-21 and one of its downstream target genes, programmed cell death 4 (PDCD4), in CD3 T cells isolated from the colonic mucosa of patients with active IBD, inactive IBD, and non-IBD controls...
November 7, 2016: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/27725205/methylated-urolithin-a-the-modified-ellagitannin-derived-metabolite-suppresses-cell-viability-of-du145-human-prostate-cancer-cells-via-targeting-mir-21
#5
Benhong Zhou, Jing Wang, Guohua Zheng, Zhenpeng Qiu
Urolithins are bioactive ellagic acid-derived metabolites produced by human colonic microflora. Although previous studies have demonstrated the cytotoxicity of urolithins, the effect of urolithins on miRNAs is still unclear. In this study, the suppressing effects of methylated urolithin A (mUA) on cell viability in human prostate cancer DU145 cells was investigated. mUA induced caspase-dependent cell apoptosis, mitochondrial depolarization and down-regulation of Bcl-2/Bax ratio. The results showed that upon exposure to mUA, miR-21 expression was decreased and the expression of PTEN and Pdcd4 protein was elevated...
October 8, 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27688683/the-effect-and-mechanism-of-action-of-metformin-on-in%C3%A2-vitro-fadu-cell-proliferation
#6
Ruijie Sun, Xiaojie Ma, Xiaolan Cai, Xinliang Pan, Dayu Liu
OBJECTIVE: To investigate the effect and mechanism of action of metformin on proliferation of a human hypopharyngeal carcinoma cell line (FaDu). METHODS: FaDu cells were treated with metformin (25-125 mmol/l). Cell proliferation was evaluated via CCK-8 assay. Real-time quantitative reverse transcription-polymerase chain reaction was used to evaluate microRNA (miR)-21-5p and PDCD4 (programmed cell death 4) expression. PDCD4 protein was quantified by Western blot...
September 29, 2016: Journal of International Medical Research
https://www.readbyqxmd.com/read/27608614/swimming-training-affects-apoptosis-related-micrornas-and-reduces-cardiac-apoptosis-in-mice
#7
Yongcai Zhao, Zhiyong Ma
We sought to investigate effects of exercise training on apoptosis-related microRNAs (miRs) and their validated targets, discussing molecular mechanism of the exercise-induced benefit in heart. Male C57BL/6 mice were randomly assigned to three groups: sedentary (SE), exercise training 1 (ET1) and exercise training 2 (ET2). ET1 swam for 8 weeks, once a day and 5 days per week with incremental load. ET2 performed the same work as ET1 and switched to twice a day by the end of the 5th week. In ET2, positive cell rate (%) tested by TUNEL assay decreased significantly (p < 0...
September 9, 2016: General Physiology and Biophysics
https://www.readbyqxmd.com/read/27600360/mir-21-inhibitor-suppressed-the-progression-of-retinoblastoma-via-the-modulation-of-pten-pi3k-akt-pathway
#8
Fu Gui, Zhengdong Hong, Zhipeng You, Hongxi Wu, Yulan Zhang
MicroRNA-21 (miR-21) was reported to act as an oncogene during the development of many human tumors. However, little was revealed about the function of miR-21 in retinoblastoma (RB). In this study, we examined the expression of miR-21 in RB tissues and explored the relationship between miR-21 and phosphatase and tensin homolog (PTEN)/phosphatidylinositol-3-OH kinase (PI3K)/AKT signal. Quantitative real-time PCR (qRT-PCR) results showed that the level of miR-21 in RB tissues was higher than that in retinal normal tissues...
September 7, 2016: Cell Biology International
https://www.readbyqxmd.com/read/27533779/microrna-21-regulates-the-erk-nf-%C3%AE%C2%BAb-signaling-pathway-to-affect-the-proliferation-migration-and-apoptosis-of-human-melanoma-a375-cells-by-targeting-spry1-pdcd4-and-pten
#9
Xu-Hua Mao, Min Chen, Yan Wang, Pan-Gen Cui, Si-Bian Liu, Zei-Yong Xu
This study aims to explore the effects of microRNA-21 (miR-21) and ERK/NF-κB signaling pathway on human melanoma A375 cells. The melanoma tissues and adjacent normal tissues were obtained from 45 melanoma patients. qRT-PCR was conducted to quantify the expression of miR-21 and the gene mRNA expressions. Human melanoma A375 cells were divided into the Mock, negative control (NC), miR-21 inhibitors, miR-21 inhibitors + siRNA-SPRY1, miR-21 inhibitors + siRNA-PDCD4, and miR-21 inhibitors + siRNA-PTEN groups...
August 17, 2016: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/27431939/tetramethylpyrazine-enhances-functional-recovery-after-contusion-spinal-cord-injury-by-modulation-of-microrna-21-fasl-pdcd4-and-pten-expression
#10
Jiang-Hu Huang, Yong Cao, Lei Zeng, Guan Wang, Min Cao, Hong-Bin Lu, Jian-Zhong Hu
Our previous study showed Tetramethylpyrazine (TMP) has protective effects against SCI. In this study, we aimed to uncover the mechanism underlying the protective effects of TMP in SCI. SCI was induced in Sprague-Dawley rats with a modified weight-drop device. One group was subjected to SCI in combination with TMP administration at a dose of 200mg/kgd, for 3 days. Concurrently, another group received SCI in combination with an equal volume of 0.9% saline. Locomotor functional recovery was assessed during the 4 weeks post-injury by performing the Basso, Beattie, and Bresnahan (BBB) rating procedure...
October 1, 2016: Brain Research
https://www.readbyqxmd.com/read/27428511/targeted-inhibition-of-oncogenic-mir-21-maturation-with-designed-rna-binding-proteins
#11
Yu Chen, Fan Yang, Lorena Zubovic, Tom Pavelitz, Wen Yang, Katherine Godin, Matthew Walker, Suxin Zheng, Paolo Macchi, Gabriele Varani
The RNA recognition motif (RRM) is the largest family of eukaryotic RNA-binding proteins. Engineered RRMs with well-defined specificity would provide valuable tools and an exacting test of the current understanding of specificity. We have redesigned the specificity of an RRM using rational methods and demonstrated retargeting of its activity in cells. We engineered the conserved RRM of human Rbfox proteins to specifically bind to the terminal loop of a microRNA precursor (pre-miR-21) with high affinity and inhibit its processing by Drosha and Dicer...
September 2016: Nature Chemical Biology
https://www.readbyqxmd.com/read/27422860/microrna-21-contributes-to-orthodontic-tooth-movement
#12
N Chen, B D Sui, C H Hu, J Cao, C X Zheng, R Hou, Z K Yang, P Zhao, Q Chen, Q J Yang, Y Jin, F Jin
microRNAs could be mechanosensitive and emerge as critical posttranscriptional regulators in the bone-remodeling process. During orthodontic tooth movement (OTM), the application of mechanical force induces alveolar bone remodeling, but whether microRNAs respond to orthodontic force and contribute to OTM is unknown. microRNA-21 (miR-21) has been previously reported in vitro to mediate stretch-induced osteogenic differentiation of periodontal ligament stem cells and support osteoclast differentiation. In this study, the authors show that miR-21 responded to orthodontic force in periodontal tissue in a dose- and time-dependent manner and regulated the osteogenesis of human periodontal ligament stem cells following OTM...
June 28, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27355932/anti-cancer-effect-of-r3v6-peptide-mediated-delivery-of-an-anti-microrna-21-antisense-oligodeoxynucleotide-in-a-glioblastoma-animal-model
#13
Binna Oh, Hojung Song, Dahee Lee, Jungju Oh, Gyeungyun Kim, Sung-Hee Ihm, Minhyung Lee
MicroRNA-21 (miR-21) expression in glioblastoma inhibits the expression of pro-apoptotic genes, thereby promoting tumor growth. In a previous study, it was shown that the amphiphilic R3V6 peptide is an efficient carrier of the anti-miR-21 antisense oligodeoxynucleotide (antisense-ODN) into cells in vitro. In the current study, in vivo delivery of antisense-ODN using the R3V6 peptide was evaluated in a glioblastoma animal model. In vitro transfection showed that the R3V6 peptide delivered antisense-ODN more efficiently than polyethylenimine (25 kDa, PEI25k) in C6 glioblastoma cells...
June 29, 2016: Journal of Drug Targeting
https://www.readbyqxmd.com/read/27355827/a-conserved-microrna-regulatory-circuit-is-differentially-controlled-during-limb-appendage-regeneration
#14
Benjamin L King, Viravuth P Yin
BACKGROUND: Although regenerative capacity is evident throughout the animal kingdom, it is not equally distributed throughout evolution. For instance, complex limb/appendage regeneration is muted in mammals but enhanced in amphibians and teleosts. The defining characteristic of limb/appendage regenerative systems is the formation of a dedifferentiated tissue, termed blastema, which serves as the progenitor reservoir for regenerating tissues. In order to identify a genetic signature that accompanies blastema formation, we employ next-generation sequencing to identify shared, differentially regulated mRNAs and noncoding RNAs in three different, highly regenerative animal systems: zebrafish caudal fins, bichir pectoral fins and axolotl forelimbs...
2016: PloS One
https://www.readbyqxmd.com/read/27344173/quercetin-inhibits-cr-vi-induced-malignant-cell-transformation-by-targeting-mir-21-pdcd4-signaling-pathway
#15
Poyil Pratheeshkumar, Young-Ok Son, Sasidharan Padmaja Divya, Lei Wang, Lilia Turcios, Ram Vinod Roy, John Andrew Hitron, Donghern Kim, Jin Dai, Padmaja Asha, Zhuo Zhang, Xianglin Shi
Hexavalent chromium [Cr(VI)] is an important human carcinogen associated with pulmonary diseases and lung cancer. Inhibition of Cr(VI)-induced carcinogenesis by a dietary antioxidant is a novel approach. Quercetin is one of the most abundant dietary flavonoids widely present in many fruits and vegetables, possesses potent antioxidant and anticancer properties. MicroRNA-21 (miR-21) is a key oncomiR significantly elevated in the majority of human cancers that exerts its oncogenic activity by targeting the tumor suppressor gene programmed cell death 4 (PDCD4)...
June 17, 2016: Oncotarget
https://www.readbyqxmd.com/read/27338031/corrigendum-inhibition-of-nf-%C3%AE%C2%BAb-by-deoxycholic-acid-induces-mir-21-pdcd4-dependent-hepatocellular-apoptosis
#16
Pedro M Rodrigues, Marta B Afonso, André L Simão, Pedro M Borralho, Cecília M P Rodrigues, Rui E Castro
No abstract text is available yet for this article.
2016: Scientific Reports
https://www.readbyqxmd.com/read/27336721/cardiac-progenitor-cell-derived-exosomes-prevent-cardiomyocytes-apoptosis-through-exosomal-mir-21-by-targeting-pdcd4
#17
J Xiao, Y Pan, X H Li, X Y Yang, Y L Feng, H H Tan, L Jiang, J Feng, X Y Yu
Cardiac progenitor cells derived from adult heart have emerged as one of the most promising stem cell types for cardiac protection and repair. Exosomes are known to mediate cell-cell communication by transporting cell-derived proteins and nucleic acids, including various microRNAs (miRNAs). Here we investigated the cardiac progenitor cell (CPC)-derived exosomal miRNAs on protecting myocardium under oxidative stress. Sca1(+)CPCs-derived exosomes were purified from conditional medium, and identified by nanoparticle trafficking analysis (NTA), transmission electron microscopy and western blotting using CD63, CD9 and Alix as markers...
2016: Cell Death & Disease
https://www.readbyqxmd.com/read/27323401/hexavalent-chromium-induces-malignant-transformation-of-human-lung-bronchial-epithelial-cells-via-ros-dependent-activation-of-mir-21-pdcd4-signaling
#18
Poyil Pratheeshkumar, Young-Ok Son, Sasidharan Padmaja Divya, Lilia Turcios, Ram Vinod Roy, John Andrew Hitron, Lei Wang, Donghern Kim, Jin Dai, Padmaja Asha, Zhuo Zhang, Xianglin Shi
Hexavalent chromium [Cr(VI)] is a well-known human carcinogen associated with an increased risk of lung cancer. However, the mechanisms underlying Cr(VI)-induced carcinogenesis remain unclear. MicroRNA-21 (miR-21) is a key regulator of oncogenic processes. Studies have shown that miR-21 exerts its oncogenic activity by targeting the tumor suppressor gene programmed cell death 4 (PDCD4). The present study examined the role of miR-21-PDCD4 signaling in Cr(VI)-induced cell transformation and tumorigenesis. Results showed that Cr(VI) induces ROS generation in human bronchial epithelial (BEAS-2B) cells...
June 13, 2016: Oncotarget
https://www.readbyqxmd.com/read/27240294/effect-of-microrna-21-on-the-proliferation-of-human-degenerated-nucleus-pulposus-by-targeting-programmed-cell-death-4
#19
B Chen, S G Huang, L Ju, M Li, F F Nie, Y Zhang, Y H Zhang, X Chen, F Gao
This study aims to explore the effect of microRNA-21 (miR-21) on the proliferation of human degenerated nucleus pulposus (NP) by targeting programmed cell death 4 (PDCD4) tumor suppressor. NP tissues were collected from 20 intervertebral disc degeneration (IDD) patients, and from 5 patients with traumatic spine fracture. MiR-21 expressions were tested. NP cells from IDD patients were collected and divided into blank control group, negative control group (transfected with miR-21 negative sequences), miR-21 inhibitor group (transfected with miR-21 inhibitors), miR-21 mimics group (transfected with miR-21 mimics) and PDCD4 siRNA group (transfected with PDCD4 siRNAs)...
May 24, 2016: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/27179559/significance-of-microrna-21-in-gastric-cancer
#20
Durairaj Sekar, Ramalingam Krishnan, Krishnaraj Thirugnanasambantham, Baskaran Rajasekaran, Villianur Ibrahim Hairul Islam, Punitha Sekar
Despite promising developments of treatment, the mortality due to gastric cancer remains high and the mechanisms of gastric cancer initiation and the development also remains elusive. It has been reported that patients with positive serologic tests for H. pylori have a higher risk of the development of gastric cancer. microRNAs (miRNAs) are short non-coding RNA molecules consisting of 21-25 nucleotides (nt) in length. The miRNAs silence their cognate target genes by inhibiting mRNA translation or degrading the mRNA molecules by binding to their 3'-untranslated (UTR) regions and plays a very important role in cancer biology...
May 11, 2016: Clinics and Research in Hepatology and Gastroenterology
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