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https://www.readbyqxmd.com/read/28738662/highly-efficient-enrichment-of-low-abundance-intact-proteins-by-core-shell-structured-fe3o4-chitosan-graphene-composites
#1
Peng Zhang, Xiaoni Fang, Guoquan Yan, Mingxia Gao, Xiangmin Zhang
In proteomics research, the screening and monitoring of disease biomarkers is still a major challenge, mainly due to their low concentration in biological samples. However, the universal enrichment of intact proteins has not been further studied. In this work, we developed a Fe3O4-chitosan@graphene (Fe3O4-CS@G) core-shell composite to enrich low-abundance proteins from biological samples. Fe3O4-CS@G composite holds chitosan layer decorated Fe3O4 core, which improves the hydrophilicity of materials greatly. Meanwhile, the graphene nanosheets shell formed via electrostatic assembly endows the composite with huge surface area (178m(2)/g)...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738638/mixed-matrix-membranes-incorporated-with-ln-mof-for-selective-and-sensitive-detection-of-nitrofuran-antibiotics-based-on-inner-filter-effect
#2
Feng Zhang, Hua Yao, Yifan Zhao, Xing Li, Gaowei Zhang, Yangyi Yang
The detection of antibiotics is critical and challenging due to the pervasive use of antibiotics had inevitably brought some negative impacts on ecosystem and human health. Herein, we reported an anti-interfere ability enhanced luminescent sensor of lanthanide metal-organic framework (Ln-MOF) filled mixed matrix membranes (MMMs), which combining the processibility of poly(methyl methacrylate) (PMMA) polymer with Ln-MOF of {[Tb2(AIP)2(H2O)10]·(AIP)·4H2O}n (Tb-AIP, where AIP is 5-aminoisophthalate) fillers...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738628/mip-meps-based-sensing-strategy-for-the-selective-assay-of-dimethoate-application-to-wheat-flour-samples
#3
D Capoferri, M Del Carlo, N Ntshongontshi, E I Iwuoha, M Sergi, F Di Ottavio, D Compagnone
The aim of this work was to demonstrate the potentialities of the use of a molecularly imprinted (MIP) sensor coupled to a microextraction by packed sorbent (MEPS) strategy for the selective and sensitive detection of dimethoate in real samples. A dimethoate-polypyrrole MIP film was realised by cyclic voltammetry (CV) on the surface of a glassy carbon electrode (GCE). Being dimethoate electro-inactive, K3[Fe(CN)6] was used as probe for the indirect quantification of the analyte via the decrease of redox peaks observed upon binding of the target analyte...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738590/extracellular-matrix-characterization-in-plaques-from-carotid-endarterectomy-by-a-proteomics-approach
#4
N Ucciferri, S Rocchiccioli, L Comelli, M Marconi, M Ferrari, G Pelosi, A Cecchettini
Extracellular matrix (ECM) is a fundamental component of multicellular organisms. Alteration of its structure and/or molecular composition are associated with several pathologies, among the others with atherosclerosis. To determine how the overall ECM architecture of a tissue as complex as the atheroma may change under varying pathological conditions constitutes a great technical challenge. This entails removing cell components and solubilisation of fibrillar proteins in order to allow enzymatic digestion and mass spectrometry analyses...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738588/maldi-matrix-assisted-laser-desorption-ionization-imaging-mass-spectrometry-ims-of-skin-aspects-of-sample-preparation
#5
Cristiana Santos de Macedo, David M Anderson, Kevin L Schey
MALDI (matrix assisted laser desorption ionization) Imaging Mass Spectrometry (IMS) allows molecular analysis of biological materials making possible the identification and localization of molecules in tissues, and has been applied to address many questions on skin pathophysiology, as well as on studies about drug absorption and metabolism. Sample preparation for MALDI IMS is the most important part of the workflow, comprising specimen collection and preservation, tissue embedding, cryosectioning, washing, and matrix application...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738526/the-potential-of-chrysophanol-in-protecting-against-high-fat-induced-cardiac-injury-through-nrf2-regulated-anti-inflammation-anti-oxidant-and-anti-fibrosis-in-nrf2-knockout-mice
#6
Yonggang Lian, Xiangjun Xia, Haiying Zhao, Yunfeng Zhu
Cardiovascular complications induced by high fat are one of the most challenging health problems presently. Oxidative stress, inflammation and fibrosis are major components included in the pathology of diabetic cardiomyopathy. Nuclear factor erythroid 2-related factor 2 (Nrf2) has been reported as an essential therapeutic target in various diseases for its induction of anti-oxidant enzymes, and other protective enzymes. Additionally, a number of Nrf2 activators showed strong anti-inflammatory properties. In the study, we calculated the Nrf2-dependent anti-oxidant, anti-inflammatory and anti-fibrosis effects of chrysophanol in high fat diet-induced diabetic heart injury...
July 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738525/mesenchymal-stem-cells-with-igf-1-and-tgf-%C3%AE-1-in-laminin-gel-for-osteochondral-defects-in-rabbits
#7
Mudasir Bashir Gugjoo, Amarpal, Ahmed Abdelbaset-Ismail, Hari Prasad Aithal, Prakash Kinjavdekar, Abhijit Motiram Pawde, Gutulla Sai Kumar, Gutulla Taru Sharma
OBJECTIVE: Healing of articular cartilage is still a challenge due to its limited potential to regenerate. In the present study, we evaluated allogenic bone marrow mesenchymal stem cells (BM-MSCs) alone or in combination with growth factors, insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1) in laminin scaffolds for healing of osteochondral defects. DESIGN: Osteochondral defects of 4mm (diameter) x 5mm (depth) were induced in the rabbit knee joints and treated with phosphate-buffered saline (PBS; control), BM-MSCs, BM-MSCs in laminin, BM-MSCs in laminin with IGF-1, or BM-MSCs in laminin with IGF-1 and TGF-β1 in 10 animals each...
July 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738520/dissolving-microneedles-for-transdermal-drug-delivery-advances-and-challenges
#8
REVIEW
Kevin Ita
Over the last number of years, a significant body of evidence has shown the benefit of using dissolving microneedles (DMNs) for transdermal drug delivery. These devices are prepared from a wide range of materials such as sugars and polymers. DMNs are mainly fabricated by micromolding, photopolymerization, drawing lithography and droplet-airborne blowing. In this review, we have focused on the advances made in the field in recent years using a representative set of studies. Although the list of studies is not exhaustive, they highlight the challenges encountered such as the need to increase mechanical strength as well as medication dose while ensuring fast release of the active ingredient...
July 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738506/taxonomy-or-trait-based-ecological-assessment-for-tropical-rivers-case-study-on-benthic-diatoms-in-mayotte-island-france-indian-ocean
#9
Kálmán Tapolczai, Agnès Bouchez, Csilla Stenger-Kovács, Judit Padisák, Frédéric Rimet
Diatom-based ecological quality assessment methods have been implemented and used regularly in the Water Framework Directive. These indices use the species' abundance profiles along a specific environmental gradient, which they aim to assess. However, this approach has several problematic issues including the unstable and fast-changing diatom taxonomy. The use of traits can be a solution if their responses to the environmental pressure are well-defined. Here, we developed taxonomy-based and trait-based diatom assemblage indices to assess the ecological status of riverine sites on a tropical island...
July 18, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28738479/-risk-of-bias-assessment-1-overview
#10
Z R Yang, F Sun, S Y Zhan
This paper reviews the concept of risk of bias, followed by demonstrating why assessment of risk of bias in systematic reviews should be different from that of quality of evidence, methodological quality, reporting quality, precision, and external validity. We also discuss the recent development of tools for risk of bias assessment, the problems with the tools themselves, and the challenges in using these tools. This review may help systematic reviewers understand risk of bias assessment and the use of assessment tools...
July 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28738453/-promoting-hepatitis-b-elimination-and-laying-emphasis-on-its-economics-evaluation
#11
S X Zhang
WHO has developed and issued global hepatitis B elimination program. Continuing hepatitis B immunization and conducting community based antiviral treatment for chronic hepatitis B are the most important strategies for hepatitis B elimination. According to the estimation of related cost by WHO, the cost of antiviral therapy would be very high. Great achievement has been made in hepatitis B immunization in China, but the prevalence of chronic hepatitis B is high. Elimination of hepatitis B needs a large amount of public resource, posing serious challenge to public health policy making...
July 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28738448/new-1-2-ghz-nmr-spectrometers-new-horizons
#12
EDITORIAL
Harald Schwalbe
The latest ultrahigh-field NMR spectrometers are a huge technological challenge that require large financial investments. In his Guest Editorial, Harald Schwalbe justifies the need for spectrometers with higher magnetic field strengths. The important results from previous generations of high-field NMR spectrometers are discussed, and research areas are identified that will benefit from the latest spectrometers.
July 24, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28738327/updated-concepts-on-treatment-outcomes-for-obstructive-sleep-apnea
#13
Victor Certal, Robson Capasso
Obstructive sleep apnea is a highly prevalent and poorly understood condition that not only leads to significant individual patient health damage, but carries a large societal cost secondary to excessive healthcare utilization, motor-vehicle accidents, and work absenteeism. While a significant fund of knowledge about its damaging effects on the cardiovascular system and neural pathways has been gathered over the past few decades, the translation to direct patient care still presents numerous challenges. With the exception of clear issues such as socially unacceptable snoring and breathing irregularities, the exact cause and effect relationship between airway narrowing, hypoxemia, and sleep fragmentation with subjective sleep-related complaints and neurocognitive symptoms is less clear...
2017: Advances in Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28738324/the-link-between-refractoriness-and-neuroprogression-in-treatment-resistant-bipolar-disorder
#14
Isabelle E Bauer, Jair C Soares, Salih Selek, Thomas D Meyer
Treatment refractoriness remains one of the biggest challenges in the field of bipolar disorder (BD) as treatments are often suboptimal or unsatisfactory. Recent evidence points towards a potential link between the progressively evolving nature of BD, increased inflammation, and reduced treatment response. There are several medications and other somatic treatments available, but remission rates are low, and medication compliance is still problematic. Psychotherapeutic techniques appear to be promising in several disease states and in relapse prevention, but additional research is needed to determine who will benefit from what strategy the most...
July 24, 2017: Modern Trends in Pharmacopsychiatry
https://www.readbyqxmd.com/read/28738319/meeting-report-intercellular-interactions-in-context-towards-a-mechanistic-understanding-of-cells-in-organs
#15
David Bryant, Aaron Johnson
The Company of Biologists held the workshop 'Intercellular interactions in context: towards a mechanistic understanding of cells in organs' at historic Wiston House in West Sussex, UK, 5-8 February 2017. The meeting brought together around 30 scientists from disparate backgrounds - yet with a common interest of how tissue morphogenesis occurs and its dysregulation leads to pathologies - to intensively discuss their latest research, the current state of the field, as well as any challenges for the future. This report summarises the concepts and challenges that arose as key questions for the fields of cell, cancer and developmental biology...
July 1, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28738262/systematic-review-of-community-health-impacts-of-mountaintop-removal-mining
#16
REVIEW
Abee L Boyles, Robyn B Blain, Johanna R Rochester, Raghavendhran Avanasi, Susan B Goldhaber, Sofie McComb, Stephanie D Holmgren, Scott A Masten, Kristina A Thayer
BACKGROUND: The objective of this evaluation is to understand the human health impacts of mountaintop removal (MTR) mining, the major method of coal mining in and around Central Appalachia. MTR mining impacts the air, water, and soil and raises concerns about potential adverse health effects in neighboring communities; exposures associated with MTR mining include particulate matter (PM), polycyclic aromatic hydrocarbons (PAHs), metals, hydrogen sulfide, and other recognized harmful substances...
July 21, 2017: Environment International
https://www.readbyqxmd.com/read/28738238/periprosthetic-seroma-with-false-positive-fdg-pet-ct-reactive-nodes-mistaken-for-metastases-in-a-patient-previously-treated-of-metastasic-melanoma-potential-source-of-diagnostic-errors
#17
Alberto Gomez Portilla, Eleder Onaindia, Maitane Larrañaga, Eduardo López de Heredia, Victor Echenagusía
FDG PET/CT is believed to be crucial in oncology, but its limited specificity represents a challenge. Prosthetic meshes used for repair abdominal defects may lead to false-positives FDG PET/CT uptake, over staging malignancies and inducing inappropriate treatments. A false-positive FDG PET/CT uptake mimicking metastatic disease during the follow-up of a previously treated metastatic melanoma patient is presented.
July 8, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28738235/treatment-decisions-in-metastatic-colorectal-cancer-beyond-first-and-second-line-combination-therapies
#18
REVIEW
A Vogel, R D Hofheinz, S Kubicka, D Arnold
Median overall survival (OS) of patients with metastatic colorectal cancer (mCRC) has reached up to 30months in recent clinical trials of first line therapies. Following disease progression after the standard in both, 1st and 2nd line, combination chemotherapy with monoclonal antibodies, many patients maintain a good performance status and a significant proportion is motivated to undergo further therapy. Choices of treatment beyond the second line setting for mCRC are therefore becoming increasingly important...
May 4, 2017: Cancer Treatment Reviews
https://www.readbyqxmd.com/read/28738203/early-detection-monitoring-for-aquatic-non-indigenous-species-optimizing-surveillance-incorporating-advanced-technologies-and%C3%A2-identifying-research-needs
#19
REVIEW
Anett S Trebitz, Joel C Hoffman, John A Darling, Erik M Pilgrim, John R Kelly, Emily A Brown, W Lindsay Chadderton, Scott P Egan, Erin K Grey, Syed A Hashsham, Katy E Klymus, Andrew R Mahon, Jeffrey L Ram, Martin T Schultz, Carol A Stepien, James C Schardt
Following decades of ecologic and economic impacts from a growing list of nonindigenous and invasive species, government and management entities are committing to systematic early- detection monitoring (EDM). This has reinvigorated investment in the science underpinning such monitoring, as well as the need to convey that science in practical terms to those tasked with EDM implementation. Using the context of nonindigenous species in the North American Great Lakes, this article summarizes the current scientific tools and knowledge - including limitations, research needs, and likely future developments - relevant to various aspects of planning and conducting comprehensive EDM...
July 21, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28738152/natural-products-and-their-mimics-as-targets-of-opportunity-for-discovery
#20
Stephen F Martin
Diverse structural types of natural products and their mimics have served as targets of opportunity in our laboratory to inspire the discovery and development of new methods and strategies to assemble polyfunctional and polycyclic molecular architectures. Furthermore, our efforts toward identifying novel compounds having useful biological properties led to the creation of new targets, many of which posed synthetic challenges that required the invention of new methodology. In this Perspective, selected examples of how we have exploited a diverse range of natural products and their mimics to create, explore, and solve a variety of problems in chemistry and biology will be discussed...
July 24, 2017: Journal of Organic Chemistry
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