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uterine nk cells

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https://www.readbyqxmd.com/read/28434167/analysis-of-s1p-receptor-expression-by-uterine-immune-cells-using-standardized-multi-parametric-flow-cytometry
#1
Jianhong Zhang, Annie Bang, Stephen J Lye
Flow cytometry is a powerful tool for phenotypic and functional analyses of single immune cells. The increasing capability of flow cytometry technology has driven a more detailed understanding of immune cell subsets and functions in complex cellular systems such as the developing human decidua/placenta. We propose a standardized procedure for the isolation and analysis of human decidual natural killer (dNK) cells and this method can be extended to investigation of other uterine lymphocytes. Here this platform is used to examine the expression of sphingosine-1-phosphate (S1P) receptor and functional growth factors by dNK cells...
April 23, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28425436/corrigendum-mhc-dependent-inhibition-of-uterine-nk-cells-impedes-fetal-growth-and-decidual-vascular-remodeling
#2
Jens Kieckbusch, Louise M Gaynor, Ashley Moffett, Francesco Colucci
No abstract text is available yet for this article.
April 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28417191/mapping-of-estradiol-binding-sites-through-receptor-micro-autoradiography-in-the-endometrial-stroma-of-early-pregnant-mice
#3
Telma M T Zorn, Rodolfo R Favaro, Mauricio Soto-Suazo, Walter E Stumpf
Estradiol triggers key biological responses in the endometrium, which rely on the presence and levels of its cognate receptors on target cells. Employing the receptor micro-autoradiography (RMAR) technique, we aimed to provide a temporal and spatial map of the functional binding sites for estradiol in the mouse endometrial stroma during early pregnancy. Uterine samples from days 1.5 to 7.5 of pregnancy were collected 1 h after tritiated- (3H-) estradiol administration and prepared for RMAR analysis. Autoradiographic incorporation of 3H-thymidine (after 1-h pulse) was evaluated over the same gestational interval...
April 17, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28414293/inherited-gins1-deficiency-underlies-growth-retardation-along-with-neutropenia-and-nk-cell-deficiency
#4
Julien Cottineau, Molly C Kottemann, Francis P Lach, Young-Hoon Kang, Frédéric Vély, Elissa K Deenick, Tomi Lazarov, Laure Gineau, Yi Wang, Andrea Farina, Marie Chansel, Lazaro Lorenzo, Christelle Piperoglou, Cindy S Ma, Patrick Nitschke, Aziz Belkadi, Yuval Itan, Bertrand Boisson, Fabienne Jabot-Hanin, Capucine Picard, Jacinta Bustamante, Céline Eidenschenk, Soraya Boucherit, Nathalie Aladjidi, Didier Lacombe, Pascal Barat, Waseem Qasim, Jane A Hurst, Andrew J Pollard, Holm H Uhlig, Claire Fieschi, Jean Michon, Vladimir P Bermudez, Laurent Abel, Jean-Pierre de Villartay, Frédéric Geissmann, Stuart G Tangye, Jerard Hurwitz, Eric Vivier, Jean-Laurent Casanova, Agata Smogorzewska, Emmanuelle Jouanguy
Inborn errors of DNA repair or replication underlie a variety of clinical phenotypes. We studied 5 patients from 4 kindreds, all of whom displayed intrauterine growth retardation, chronic neutropenia, and NK cell deficiency. Four of the 5 patients also had postnatal growth retardation. The association of neutropenia and NK cell deficiency, which is unusual among primary immunodeficiencies and bone marrow failures, was due to a blockade in the bone marrow and was mildly symptomatic. We discovered compound heterozygous rare mutations in Go-Ichi-Ni-San (GINS) complex subunit 1 (GINS1, also known as PSF1) in the 5 patients...
April 17, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28413491/upregulated-unique-long-16-binding-protein-1-detected-in-preeclamptic-placenta-affects-human-extravillous-trophoblast-cell-line-htr-8-svneo-invasion-by-modulating-the-function-of-uterine-natural-killer-cells
#5
Jing Liu, Guang Song, Xuewen Lin, Xining Pang, Tao Meng
Well-controlled trophoblast invasion at the maternal-fetal interface is crucial for normal placentation and successful pregnancy, otherwise pathological conditions of pregnancy occur, such as preeclampsia. In previous studies, it has been demonstrated that unique long 16 binding protein (ULBP)1, a ligand for the natural-killer group (NKG)2D receptor on uterine natural killer (uNK) cells, is upregulated in the placenta in patients with preeclampsia. As they are present on the majority of the decidua, uNK have an important role in pregnancy...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28396316/extravillous-trophoblast-and-endothelial-cell-crosstalk-mediates-leukocyte-infiltration-to-the-early-remodeling-decidual-spiral-arteriole-wall
#6
Ruhul H Choudhury, Caroline E Dunk, Stephen J Lye, John D Aplin, Lynda K Harris, Rebecca L Jones
Decidual spiral arteriole (SpA) remodeling is essential to ensure optimal uteroplacental blood flow during human pregnancy, yet very little is known about the regulatory mechanisms. Uterine decidual NK (dNK) cells and macrophages infiltrate the SpAs and are proposed to initiate remodeling before colonization by extravillous trophoblasts (EVTs); however, the trigger for their infiltration is unknown. Using human first trimester placenta, decidua, primary dNK cells, and macrophages, we tested the hypothesis that EVTs activate SpA endothelial cells to secrete chemokines that have the potential to recruit maternal immune cells into SpAs...
April 10, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28395334/natural-killer-cell-deficiency-alters-placental-development-in-rats
#7
Stephen J Renaud, Regan L Scott, Damayanti Chakraborty, Mohammad A K Rumi, Michael J Soares
Natural killer (NK) cells are the most prevalent leukocyte population in the uterus during early pregnancy. Natural killer cells contribute to uterine vascular (spiral artery) remodeling in preparation for the increased demand on these vessels later in pregnancy. A second wave of spiral artery modification is directed by invasive trophoblast cells. The significance of the initial wave of NK-cell-mediated vascular remodeling in species exhibiting deep trophoblast invasion such as humans and rats is not known...
January 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28388469/higher-prevalence-of-colonization-with-gardnerella-vaginalis-and-gram-negative-anaerobes-in-patients-with-recurrent-miscarriage-and-elevated-peripheral-natural-killer-cells
#8
R J Kuon, R Togawa, K Vomstein, M Weber, T Goeggl, T Strowitzki, U R Markert, S Zimmermann, V Daniel, A H Dalpke, B Toth
The role of vaginal infections in recurrent miscarriage (RM) is discussed controversially and screening is not recommended in international guidelines. Peripheral and uterine NK cells (pNK, uNK) play an important role in the establishment of a healthy pregnancy and are targets of immune diagnostics in RM patients. The aim of this study was to analyze the composition of the vaginal microbiota in RM patients and to correlate the findings to clinical characteristics as well as NK cell parameters. In total, n=243 RM patients with ≥3 consecutive miscarriages were recruited between 11/2011 and 03/2016...
March 30, 2017: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/28369855/in-vivo-activation-of-invariant-natural-killer-t-cells-induces-systemic-and-local-alterations-in-t-cell-subsets-prior-to-preterm-birth
#9
Nardhy Gomez-Lopez, Roberto Romero, Marcia Arenas-Hernandez, George Schwenkel, Derek St Louis, Sonia S Hassan, Tara N Mial
Preterm birth, the leading cause of neonatal morbidity and mortality worldwide, is frequently preceded by spontaneous preterm labor, a syndrome of multiple etiologies. Pathological inflammation is causally linked to spontaneous preterm labor. Indeed, direct activation of invariant natural killer T (iNKT) cells via α-galactosylceramide induces preterm labor/birth largely by initiating systemic and local (i.e., decidua and myometrium) innate immune responses. Herein, we investigated whether iNKT-cell activation altered local and systemic T-cell subsets...
April 2, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28327604/chymase-producing-cells-of-the-innate-immune-system-are-required-for-decidual-vascular-remodeling-and-fetal-growth
#10
Nicole Meyer, Katja Woidacki, Martin Knöfler, Gudrun Meinhardt, Désirée Nowak, Philipp Velicky, Jürgen Pollheimer, Ana C Zenclussen
Intrauterine growth restriction (IUGR) is caused by insufficient remodeling of spiral arteries (SAs). The mechanism underlying the relevance of natural killer cells (NKs) and mast cells (MCs) for SA remodeling and its effects on pregnancy outcome are not well understood. We show that NK depletion arrested SA remodeling without affecting pregnancy. MC depletion resulted in abnormally remodeled SAs and IUGR. Combined absence of NKs and MCs substantially affected SA remodeling and impaired fetal growth. We found that α-chymase mast cell protease (Mcpt) 5 mediates apoptosis of uterine smooth muscle cells, a key feature of SA remodeling...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28318556/natural-killer-cells-in-placentation-and-cancer-implications-for-hypertension-during-pregnancy
#11
Heather Yvonne Small, Denise C Cornelius, Tomasz J Guzik, Christian Delles
Hypertension during pregnancy is the most common medical condition encountered during gestation. Despite this, knowledge of the mechanisms that underlie the disease and the development of new therapies are limited. Hypertension during pregnancy and some forms of cancer confer an increased risk to the development of cardiovascular disease later in life; one mechanism which may link these conditions is the involvement of natural killer (NK) cells. Whilst immunology and immunotherapy are well-developed areas in oncology; the complex mechanisms of the immune system in health and disease at the maternal-fetal interface are less well-defined...
March 7, 2017: Placenta
https://www.readbyqxmd.com/read/28295343/epithelial-membrane-protein-2-emp2-deficiency-alters-placental-angiogenesis-mimicking-features-of-human-placental-insufficiency
#12
Carmen J Williams, Alison Chu, Wendy N Jefferson, David Casero, Deepthi Sudhakar, Nevil Khurana, Claire P Hogue, Chinmayi Aryasomayajula, Priya Patel, Peggy Sullivan, Elizabeth Padilla-Banks, Shabnam Mohandessi, Carla Janzen, Madhuri Wadehra
Epithelial membrane protein-2 (EMP2) is a tetraspan protein predicted to regulate placental development. Highly expressed in secretory endometrium and trophectoderm cells, previous studies suggest that it may regulate implantation by orchestrating the surface expression of integrins and other membrane proteins. In order to test the role of EMP2 in pregnancy, mice lacking EMP2 (Emp2(-/-) ) were generated. Emp2(-/-) females are fertile but have reduced litter sizes when carrying Emp2(-/-) but not Emp2(+/-) fetuses...
March 14, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28284455/uterine-nk-cells-and-macrophages-in-pregnancy
#13
Marijke M Faas, Paul de Vos
The presence of immune cells in the placental bed is important for both mother and child. Although various immune cells can be found in the placental bed, such as regulatory T cells and dendritic cells, uterine NK cells and macrophages are the most prominent immune cells in the placental bed in early pregnancy. uNK cell and macrophage numbers in the placental bed decrease in the third trimester. These cells seem to be specifically adapted for their function and environment. uNK cells do not show cytotoxic activity, but are producers of cytokines, growth factors and many other factors...
March 2, 2017: Placenta
https://www.readbyqxmd.com/read/28283683/pre-pregnancy-levels-of-peripheral-natural-killer-cells-as-markers-for-immunomodulatory-treatment-in-patients-with-recurrent-miscarriage
#14
Ruben J Kuon, Franziska Müller, Kilian Vomstein, Maja Weber, Hannes Hudalla, Sabine Rösner, Thomas Strowitzki, Udo Markert, Volker Daniel, Bettina Toth
Immunological risk factors in patients with recurrent miscarriage (RM) are discussed controversially. Abnormalities of natural killer cells (NK) have been described in RM patients. Lipid infusions are known to modulate lymphocyte subsets. The aim of this study was to identify immune parameters that predict success of treatment with lipid infusions in RM patients with elevated NK. In sum, n = 341 couples with RM were screened for established risk factors and peripheral lymphocyte subpopulations as well as uterine NK cells...
March 11, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28280497/activation-of-blood-cd3-cd56-cd8-t-cells-during-pregnancy-and-multiple-sclerosis
#15
Clara de Andrés, Lidia Fernández-Paredes, Marta Tejera-Alhambra, Bárbara Alonso, Rocío Ramos-Medina, Silvia Sánchez-Ramón
A striking common feature of most autoimmune diseases is their female predominance, with at least twice as common among women than men in relapsing-remitting multiple sclerosis (MS), the prevailing MS clinical form with onset at childbearing age. This fact, together with the protective effect on disease activity during pregnancy, when there are many biological changes including high levels of estrogens and progesterone, puts sex hormones under the spotlight. The role of natural killer (NK) and NKT cells in MS disease beginning and course is still to be elucidated...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28185362/definitive-class-i-human-leukocyte-antigen-expression-in-gestational-placentation-hla-f-hla-e-hla-c-and-hla-g-in-extravillous-trophoblast-invasion-on-placentation-pregnancy-and-parturition
#16
Rinat Hackmon, Lakmini Pinnaduwage, Jianhong Zhang, Stephen J Lye, Daniel E Geraghty, Caroline E Dunk
PROBLEM: The extravillous trophoblasts (EVT) express HLA-C and HLA-G, but HLA-E and HLA-F are the subject of conflicting reports. In this study, we define the HLA expression profile during active EVT placental implantation, pregnancy development, and parturition. METHOD OF STUDY: Immunohistochemistry, q-PCR, and Western blot were used to investigate HLA-C, HLA-E, and HLA-F placental expression across gestation from the early first trimester, late first trimester, second trimester (n=10 in each), preterm gestation (n=6) to elective term cesarean section and term vaginal deliveries (n=12, 38-41 weeks)...
February 10, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/28161567/multi-resolution-cell-orientation-congruence-descriptors-for-epithelium-segmentation-in-endometrial-histology-images
#17
Guannan Li, Shan E Ahmed Raza, Nasir M Rajpoot
It has been recently shown that recurrent miscarriage can be caused by abnormally high ratio of number of uterine natural killer (UNK) cells to the number of stromal cells in human female uterus lining. Due to high workload, the counting of UNK and stromal cells needs to be automated using computer algorithms. However, stromal cells are very similar in appearance to epithelial cells which must be excluded in the counting process. To exclude the epithelial cells from the counting process it is necessary to identify epithelial regions...
January 22, 2017: Medical Image Analysis
https://www.readbyqxmd.com/read/28161059/dnk-derived-ifn-%C3%AE-mediates-vsmc-migration-and-apoptosis-via-the-induction-of-lncrna-meg3-a-role-in-uterovascular-transformation
#18
Weifang Liu, Xiaoxia Liu, Minglian Luo, Xiaoping Liu, Qingqing Luo, Hui Tao, Di Wu, Sisi Lu, Jing Jin, Yin Zhao, Li Zou
INTRODUCTION: Appropriate spiral artery remodeling is critical for successful fetal development and pregnancy outcomes. The vascular smooth muscle cell (VSMC) loss and separation, involving cell apoptosis and migration, plays an important role in this process. Decidual natural killer cells (dNK)-derived interferon gamma (IFN-γ), a key regulator of uterine arterial remodeling, can facilitate separation of VSMC layers, however, the specific mechanisms of it action are unknown. Long non-coding RNA MEG3 functions as tumor suppressor by regulating apoptosis and migration...
February 2017: Placenta
https://www.readbyqxmd.com/read/28110751/novel-regulators-of-hemodynamics-in-the-pregnant-uterus
#19
N C Clark, C A Pru, J K Pru
The uterus is a highly dynamic organ, undergoing dramatic physiological changes during normal cyclicity and pregnancy. Many of these changes involve remodeling of the uterine vasculature in order to provide oxygen and nutrients to the developing embryo/fetus. Vasculogenesis, angiogenesis, vasodilation/vasoconstriction, and vascular permeability are coordinated by a vast network of autocrine, paracrine, and endocrine-signaling factors that derive from a number of cellular sources at the maternal:fetal interface, as well as from tissue outside the uterus...
2017: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/28098245/natural-cytotoxicity-receptor-1-in-mouse-unk-cell-maturation-and-function
#20
A M Felker, B A Croy
Early and midgestational decidua of mice genetically ablated for expression of the natural killer (NK) cell natural cytotoxicity receptor (NCR; Ncr1(Gfp/Gfp) mice) shows restricted angiogenesis and atypically small uterine (u)NK cells . We hypothesized that NCR1 inactivation disturbs maturation and angiokine production by uterine natural killer (uNK) cells. Using histological and morphometric approaches, we observed that Ncr1(Gfp/Gfp) but not control C57BL/6 (B6) implantation sites sustain immature, non-granulated uNK cells into midpregnancy...
January 18, 2017: Mucosal Immunology
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