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Decellularized matrix

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https://www.readbyqxmd.com/read/28639560/mechanical-properties-of-decellularized-extracellular-matrix-coated-with-ticapcon-film
#1
I V Sukhorukova, A N Sheveyko, K L Firestein, Ph V Kiryukhantsev-Korneev, D Golberg, D V Shtansky
For the first time the surface of decellularized extracellular matrix (DECM) was modified via deposition of a multicomponent bioactive nanostructured film for improvement of the DECM's mechanical properties. TiCaPCON films were deposited onto the surface of intact and decellularized ulna, radius, and humerus bones by magnetron sputtering of TiC0.5 + 10%Ca3(PO4)2 and Ti targets in a gaseous mixture of Ar + N2. The film structure was studied using x-ray diffraction, scanning and transmission electron microscopy, and Raman spectroscopy...
June 22, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28634932/preparation-of-decellularized-biological-scaffolds-for-3d-cell-culture
#2
Bryan N Brown, Michael J Buckenmeyer, Travis A Prest
The biggest challenge of designing and implementing an in vitro study is developing a microenvironment that most closely represents the interactions observed in vivo. Decellularization of tissues and organs has been shown to be an effective method for the removal of potentially immunogenic constituents while preserving essential growth factors and extracellular matrix (ECM ) proteins necessary for proper cell function. Enzymatic digestion of decellularized tissues allows these tissue-specific components to be reconstituted into bioactive hydrogels through a physical crosslinking of collagen...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28631900/-experimental-development-and-rationale-for-a-renal-decellularization-protocol-with-subsequent-comprehensive-assessment-of-the-biological-scaffold
#3
P V Glybochko, S N Alekseenko, E A Gubareva, E V Kuevda, A A Basov, A S Sotnichenko, S S Dzhimak, I S Gumenyuk, I Kh Egiev, V N Chechelyan, R Z Nakokhov, O M Lyasota, Yu V Teterin
Chronic renal failure (CRF) is one of the most challenging problems of contemporary medicine. Patients with chronic renal failure usually need renal replacement therapy as either hemodialysis, peritoneal dialysis or a kidney transplant. The latter is the most promising option for end-stage kidney disease. However, the shortage of donor organs, the complexity of their delivery, the difficulty in finding an immunologically compatible donor and the need for lifelong immunosuppression triggered advances in modern tissue engineering...
June 2017: Urologii︠a︡
https://www.readbyqxmd.com/read/28629082/influence-of-residual-composition-on-the-structure-and-properties-of-extracellular-matrix-derived-hydrogels
#4
Jesús A Claudio-Rizo, Magdalena Rangel-Argote, Laura E Castellano, Jorge Delgado, José L Mata-Mata, Birzabith Mendoza-Novelo
In this work, hydrolysates of extracellular matrix (hECM) were obtained from rat tail tendon (TR), bovine Achilles tendon (TAB), porcine small intestinal submucosa (SIS) and bovine pericardium (PB), and they were polymerized to generate ECM hydrogels. The composition of hECM was evaluated by quantifying the content of sulphated glycosaminoglycans (sGAG), fibronectin and laminin. The polymerization process, structure, physicochemical properties, in vitro degradation and biocompatibility were studied and related to their composition...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28608951/tumstatin-regulates-the-angiogenic-and-inflammatory-potential-of-airway-smooth-muscle-extracellular-matrix
#5
Louise Margaret Harkness, Markus Weckmann, Matthias Kopp, Tim Becker, Anthony Wayne Ashton, Janette Kay Burgess
The extracellular matrix (ECM) creates the microenvironment of the tissue; an altered ECM in the asthmatic airway may be central in airway inflammation and remodelling. Tumstatin is a collagen IV-derived matrikine reduced in the asthmatic airway wall that reverses airway inflammation and remodelling in small and large animal models of asthma. This study hypothesized that the mechanisms underlying the broad asthma-resolving effects of tumstatin were due to autocrine remodelling of the ECM. Neutrophils and endothelial cells were seeded on decellularized ECM of non-asthmatic (NA) or asthmatic (A) airway smooth muscle (ASM) cells previously exposed to tumstatin in the presence or absence of a broad matrix metalloproteinase inhibitor, Marimastat...
June 13, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28604702/isdot-in-situ-decellularization-of-tissues-for-high-resolution-imaging-and-proteomic-analysis-of-native-extracellular-matrix
#6
Alejandro E Mayorca-Guiliani, Chris D Madsen, Thomas R Cox, Edward R Horton, Freja A Venning, Janine T Erler
The extracellular matrix (ECM) is a master regulator of cellular phenotype and behavior. It has a crucial role in both normal tissue homeostasis and disease pathology. Here we present a fast and efficient approach to enhance the study of ECM composition and structure. Termed in situ decellularization of tissues (ISDoT), it allows whole organs to be decellularized, leaving native ECM architecture intact. These three-dimensional decellularized tissues can be studied using high-resolution fluorescence and second harmonic imaging, and can be used for quantitative proteomic interrogation of the ECM...
June 12, 2017: Nature Medicine
https://www.readbyqxmd.com/read/28602577/fabrication-of-porous-scaffolds-with-decellularized-cartilage-matrix-for-tissue-engineering-application
#7
Bita Nasiri, Shohreh Mashayekhan
Due to the avascular nature of articular cartilage, damaged tissue has little capacity for spontaneous healing. Three-dimensional scaffolds have potential for use in tissue engineering approach for cartilage repair. In this study, bovine cartilage tissue was decellularized and chemically crosslinked hybrid chitosan/extracellular matrix (ECM) scaffolds were fabricated with different ECM weight ratios by simple freeze drying method. Various properties of chitosan/ECM scaffolds such as microstructure, mechanical strength, swelling ratio, and biodegradability rate were investigated to confirm improved structural and biological characteristics of chitosan scaffolds in the presence of ECM...
June 8, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28600910/dried-human-skin-fibroblasts-as-a-new-substratum-for-functional-culture-of-hepatic-cells
#8
Agnieszka Wencel, Karolina Ewa Zakrzewska, Anna Samluk, Bartłomiej Henryk Noszczyk, Dorota Genowefa Pijanowska, Krzysztof Dariusz Pluta
The primary hepatocytes culture is still one of the main challenges in toxicology studies in the drug discovery process, development of in vitro models to study liver function, and cell-based therapies. Isolated hepatocytes display a rapid decline in viability and liver-specific functions including albumin production, conversion of ammonia to urea, and activity of the drug metabolizing enzymes. A number of methods have been developed in order to maintain hepatocytes in their highly differentiated state in vitro...
2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28600128/decellularized-tongue-tissue-as-an-in-vitro-model-for-studying-tongue-cancer-and-tongue-regeneration
#9
Long Zhao, Linxuan Huang, Shuyi Yu, Junheng Zheng, Hua Wang, Yan Zhang
The decellularization of tissues or organs provides an efficient strategy for preparing functional scaffolds for tissue engineering. The microstructures of native extracellular matrices and biochemical compositions retained in the decellularized matrices provide tissue-specific microenvironments for anchoring cells. Here, we report the tongue extracellular matrix (TEM), which showed favorable cytocompatibility for normal tongue-derived cells and tongue squamous cell carcinoma (TSCC) cells under static or stirring culture conditions...
June 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28583764/proteomic-composition-and-immunomodulatory-properties-of-urinary-bladder-matrix-scaffolds-in-homeostasis-and-injury
#10
REVIEW
Kaitlyn Sadtler, Sven D Sommerfeld, Matthew T Wolf, Xiaokun Wang, Shoumyo Majumdar, Liam Chung, Dhanashree S Kelkar, Akhilesh Pandey, Jennifer H Elisseeff
Urinary bladder matrix (UBM) is used clinically for management of wounds and reinforcement of surgical soft tissue repair, among other applications. UBM consists of the lamina propria and basal lamina of the porcine urinary bladder, and is decellularized as part of the process to manufacture the medical device. UBM is composed mainly of Collagen I, but also contains a wide variety of fibrillar and basement membrane collagens, glycoproteins, proteoglycans and ECM-associated factors. Upon application of the biomaterial in a traumatic or non-traumatic setting in a mouse model, there is a cascade of immune cells that respond to the damaged tissue and biomaterial...
June 2, 2017: Seminars in Immunology
https://www.readbyqxmd.com/read/28583182/tissue-specific-bioactivity-of-soluble-tendon-derived-and-cartilage-derived-extracellular-matrices-on-adult-mesenchymal-stem-cells
#11
Benjamin B Rothrauff, Guang Yang, Rocky S Tuan
BACKGROUND: Biological scaffolds composed of tissue-derived extracellular matrix (ECM) can promote homologous (i.e., tissue-specific) cell differentiation through preservation of biophysical and biochemical motifs found in native tissues. Solubilized ECMs derived from decellularized tendon and cartilage have recently been promoted as tissue-specific biomaterials, but whether tissue-specific bioactivity is preserved following solubilization is unknown. This study explored the tissue-specific bioactivity of soluble decellularized tendon and cartilage ECMs on human bone marrow-derived mesenchymal stem cells (MSCs) presented across different culture microenvironments, including two-dimensional (2D) tissue culture plastic, aligned electrospun nanofibers, cell pellets, and cell-seeded photocrosslinkable hydrogels...
June 5, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28576031/fabrication-of-a-three-dimensional-spongy-scaffold-using-human-wharton-s-jelly-derived-extra-cellular-matrix-for-wound-healing
#12
Bahareh Beiki, Bahman Zeynali, Ehsan Seyedjafari
The Wharton's jelly (WJ) contains significant amounts of extracellular matrix (ECM) components and rich source of endogenous growth factors. In this study, we designed a new biomimetic spongy scaffold from decellularized WJ-derived ECM and used it as a skin substitute. Histological analysis and biochemical assays showed that bio-active molecules preserved in the fabricated scaffolds and that the scaffolds have highly interconnected porous structure. Cytotoxicity and mechanical evaluation of the scaffold indicated that it is non-toxic and has appropriate mechanical properties...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28570606/effects-of-two-different-decellularization-routes-on-the-mechanical-properties-of-decellularized-lungs
#13
Jessica Julioti Urbano, Renata Kelly da Palma, Flávia Mafra de Lima, Paula Fratini, Leticia Lopes Guimaraes, Juan J Uriarte, Letícia Heineck Alvarenga, Maria Angelica Miglino, Rodolfo de Paula Vieira, Renato Araujo Prates, Daniel Navajas, Ramon Farrè, Luis Vicente Franco Oliveira
Considering the limited number of available lung donors, lung bioengineering using whole lung scaffolds has been proposed as an alternative approach to obtain lungs suitable for transplantation. However, some decellularization protocols can cause alterations on the structure, composition, or mechanical properties of the lung extracellular matrix. Therefore, the aim of this study was to compare the acellular lung mechanical properties when using two different routes through the trachea and pulmonary artery for the decellularization process...
2017: PloS One
https://www.readbyqxmd.com/read/28566689/bone-marrow-niche-mimetics-modulate-hspc-function-via-integrin-signaling
#14
Martin Kräter, Angela Jacobi, Oliver Otto, Stefanie Tietze, Katrin Müller, David M Poitz, Sandra Palm, Valentina M Zinna, Ulrike Biehain, Manja Wobus, Triantafyllos Chavakis, Carsten Werner, Jochen Guck, Martin Bornhauser
The bone marrow (BM) microenvironment provides critical physical cues for hematopoietic stem and progenitor cell (HSPC) maintenance and fate decision mediated by cell-matrix interactions. However, the mechanisms underlying matrix communication and signal transduction are less well understood. Contrary, stem cell culture is mainly facilitated in suspension cultures. Here, we used bone marrow-mimetic decellularized extracellular matrix (ECM) scaffolds derived from mesenchymal stromal cells (MSCs) to study HSPC-ECM interaction...
May 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28562183/extracellular-matrices-for-bone-regeneration-a-literature-review
#15
Alaa Mansour, Mohamed Amine Mezour, Zahi Badran, Faleh Tamimi
Bone regeneration is a major challenge in cranio-facial and orthopedic surgery. Several biomaterials have been developed to overcome the limitations of autogenic bone graft, the gold standard for bone regeneration. Among these materials, decellularized extracellular matrix (ECM) has offered promising outcomes in bone regeneration. The ECMs include bioactive molecules that modulate cell proliferation and differentiation, thus, providing an excellent microenvironment for cell recruitment. ECM can be derived from tissues such as bone, cartilage, skin, and developing tooth germs, Alternatively, it can also be derived in vitro from cells such as osteoblasts, chondrocytes, adipose stem cells (ASCs), mesenchymal cells (MSCs) and embryonic stem cells (ESCs)...
May 31, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28556592/the-effect-of-riboflavin-uva-cross-linking-on-anti-degeneration-and-promoting-angiogenic-capability-of-decellularized-liver-matrix
#16
Junxi Xiang, Peng Liu, Xinglong Zheng, Dinghui Dong, Shujuan Fan, Jian Dong, Xufeng Zhang, Xuemin Liu, Bo Wang, Yi Lv
Weak mechanical property and unstable degradation rate limited the application of decellularized liver matrix in tissue engineering. The aim of this study was to explore a new method for improving the mechanical properties, anti-degeneration and angiogenic capability of decellularized liver matrix. This was achieved by a novel approach using riboflavin/ultraviolet A treatment to induce collagen cross-linking of decellularized matrix. Histological staining and scanning electron microscope showed that the diameter of cross-linked fibers significantly increased compared with the control group...
May 27, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28554010/a-bilaminated-decellularized-scaffold-for-islet-transplantation-structure-properties-and-functions-in-diabetic-mice
#17
Xi Wang, Kai Wang, Wei Zhang, Ming Qiang, Ying Luo
Ectopic transplantation of islets provides a beta cell-replacement approach that may allow the recovery of physiological regulation of the blood sugar level in patients with Type I diabetes (T1D). In development of new extrahepatic islet transplantation protocols in support of the islet engraftment, it is pivotal to develop scaffold materials with multifaceted functions to provide beneficial microenvironment, mediate host response in favor of vascularization/islet integration and maintain long-term islet function at the transplantation site...
May 22, 2017: Biomaterials
https://www.readbyqxmd.com/read/28550502/decellularization-methods-for-scaffold-fabrication
#18
Sweta K Gupta, Narayan C Mishra
Decellularization is the process of removal of native cells from tissue, leaving behind a three-dimensional (3D) ultrastructure of extracellular matrix (ECM) proteins while preserving the bioactivity and mechanics of the tissue. It offers a unique top-down approach for fabricating ECM based natural scaffold for tissue engineering application. Herein, this chapter presents the fabrication of decellularized scaffold employing different methods: whole organ perfusion, immersion and agitation, pressure gradient, and supercritical fluid...
May 27, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28546133/tissue-engineered-trachea-regeneration-using-decellularized-trachea-matrix-treated-with-laser-micropore-technique
#19
Yong Xu, Dan Li, Zongqi Yin, Aijuan He, Miaomiao Lin, Gening Jiang, Xiao Song, Xuefei Hu, Yi Liu, Jinpeng Wang, Xiaoyun Wang, Liang Duan, Guangdong Zhou
Tissue-engineered trachea provides a promising approach for reconstruction of long segmental tracheal defects. However, a lack of ideal biodegradable scaffolds greatly restricts its clinical translation. Decellularized trachea matrix (DTM) is considered a proper scaffold for trachea cartilage regeneration owing to natural tubular structure, cartilage matrix components, and biodegradability. However, cell residual and low porosity of DTM easily result in immunogenicity and incomplete cartilage regeneration. To address these problems, a laser micropore technique (LMT) was applied in the current study to modify trachea sample porosity to facilitate decellular treatment and cell ingrowth...
May 22, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28543403/decellularized-human-liver-is-too-heterogeneous-for-designing-a-generic-extracellular-matrix-mimic-hepatic-scaffold
#20
Giorgio Mattei, Chiara Magliaro, Andrea Pirone, Arti Ahluwalia
Decellularized human livers are considered the perfect extracellular matrix (ECM) surrogate because both three-dimensional architecture and biological features of the hepatic microenvironment are thought to be preserved. However, donor human livers are in chronically short supply, both for transplantation or as decellularized scaffolds, and will become even scarcer as life expectancy increases. It is hence of interest to determine the structural and biochemical properties of human hepatic ECM to derive design criteria for engineering biomimetic scaffolds...
May 23, 2017: Artificial Organs
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