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Decellularized matrix

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https://www.readbyqxmd.com/read/27925237/ambiguity-in-the-presentation-of-decellularized-tissue-composition-the-need-for-standardized-approaches
#1
Arne A N Bruyneel, Carolyn A Carr
Decellularization offers great potential to the field of tissue engineering, as this method gives rise to scaffold material with the native organ architecture by removing all cellular material and leaving much of the extracellular matrix (ECM) intact. However, many parameters may affect decellularization efficacy and ECM retention and, therefore, decellularization protocols need to be optimized for specific needs. This requires robust methods for comparison of decellularized tissue composition. Various representation methods are used in literature to express tissue composition (DNA, glycosaminoglycans, collagen, other ECM proteins, and growth factors)...
December 7, 2016: Artificial Organs
https://www.readbyqxmd.com/read/27925229/plga-ptmc-cultured-bone-mesenchymal-stem-cell-scaffold-enhances-cartilage-regeneration-in-tissue-engineered-tracheal-transplantation
#2
Bingyang Yan, Zhipei Zhang, Xiaoping Wang, Yunfeng Ni, Yongshi Liu, Tao Liu, Wuping Wang, Hao Xing, Ying Sun, Jian Wang, Xiao-Fei Li
The treatment of long-segment tracheal defect requires the transplantation of effective tracheal substitute, and the tissue-engineered trachea (TET) has been proposed as an ideal tracheal substitute. The major cause of the failure of segmental tracheal defect reconstruction by TET is airway collapse caused by the chondromalacia of TET cartilage. The key to maintain the TET structure is the regeneration of chondrocytes in cartilage, which can secrete plenty of cartilage matrices. To address the problem of the chondromalacia of TET cartilage, this study proposed an improved strategy...
December 7, 2016: Artificial Organs
https://www.readbyqxmd.com/read/27924607/fabrication-and-characterization-of-decellularized-periodontal-ligament-cell-sheet-constructs
#3
Amro Farag, Cédryck Vaquette, Dietmar W Hutmacher, P Mark Bartold, Saso Ivanovski
Decellularized tissue-engineered constructs have the potential to promote regeneration by providing a biomimetic extracellular matrix that directs tissue-specific regeneration when implanted in situ. Recently, the use of cell sheets has shown promising results in promoting periodontal regeneration. Here, we describe the fabrication of decellularized periodontal cell sheets with intact extracellular matrix structural and biological properties. Melt electro spun polycaprolactone (PCL) scaffolds are used as a carrier for the inherently fragile cell sheets, to provide support during the processes of decellularization...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27915024/extracellular-matrix-hydrogels-from-decellularized-tissues-structure-and-function
#4
REVIEW
Lindsey T Saldin, Madeline C Cramer, Sachin S Velankar, Lisa J White, Stephen F Badylak
: Extracellular matrix (ECM) bioscaffolds prepared from decellularized tissues have been used to facilitate constructive and functional tissue remodeling in a variety of clinical applications. The discovery that these ECM materials could be solubilized and subsequently manipulated to form hydrogels expanded their potential in vitro and in vivo utility; i.e. as culture substrates comparable to collagen or Matrigel, and as injectable materials that fill irregularly-shaped defects. The mechanisms by which ECM hydrogels direct cell behavior and influence remodeling outcomes are only partially understood, but likely include structural and biological signals retained from the native source tissue...
November 30, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27905026/native-matrix-based-human-lung-alveolar-tissue-model-in-vitro-studies-of-the-reparatory-actions-of-mesenchymal-stem-cells
#5
Ieva Bruzauskaite, Jovile Raudoniute, Jaroslav Denkovskij, Edvardas Bagdonas, Sandra Meidute-Abaraviciene, Vaida Simonyte, Daiva Bironaite, Almantas Siaurys, Eiva Bernotiene, Ruta Aldonyte
Studies of lung diseases in vitro often rely on flat, plastic-based monocultures, due to short lifespan of primary cells, complicated anatomy, lack of explants, etc. We hereby present a native 3D model with cues for repopulating epithelial cells. Abilities of mesenchymal stem cells (MSC) to modulate bacterial lipopolysaccharide (LPS) and cigarette smoke-induced injury to pulmonary epithelium were tested in our model. Post-mortem human lung tissue was sliced, cut and decellularized. Resulting matrix pads were reseeded with pulmonary epithelium (A549 line)...
December 1, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27901386/comparison-of-four-methods-for-generating-decellularized-equine-synovial-extracellular-matrix
#6
Nathalie A Reisbig, Hayam A Hussein, Erin Pinnell, Alicia L Bertone
OBJECTIVE To evaluate 4 methods for generating decellularized equine synovial extracellular matrix. SAMPLE Villous synovium harvested from the femoropatellar and medial femorotibial joints of 4 healthy adult horses < 7 years of age. Synovial samples were frozen (-80°C) until used. PROCEDURES Synovial samples were thawed and left untreated (control) or decellularized with 1 of 4 methods (15 samples/horse/method): incubation in 0.1% peracetic acid (PAA), incubation in 0.1% PAA twice, incubation in 1% Triton X-100 followed by incubation in DNase, and incubation in 2M NaCl followed by incubation in DNase...
December 2016: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/27898187/tissue-engineering-acellular-scaffolds-the-significant-influence-of-physical-and-procedural-decellularization-factors
#7
F Starnecker, F Koenig, C Hagl, N Thierfelder
The importance of decellularized medical products has significantly increased during the last years. In this paper, we evaluated the effects of selected physical and procedural decellularization (DC) factors with the aim to systematically assess their influence on DC results. 72 porcine aortic walls (AW) were divided into three groups and exposed to a DC solution for 4 h and 8 h, either continuously or in repeated cycles. The AW were rocked (90bpm), whirled (10 l/min), sonicated (120W, 45 kHz) or exposed to a combination of these treatments, followed by 10 washing cycles...
November 29, 2016: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/27895039/region-specific-effect-of-the-decellularized-meniscus-extracellular-matrix-on-mesenchymal-stem-cell-based-meniscus-tissue-engineering
#8
Kazunori Shimomura, Benjamin B Rothrauff, Rocky S Tuan
BACKGROUND: The meniscus is the most commonly injured knee structure, and surgical repair is often ineffective. Tissue engineering-based repair or regeneration may provide a needed solution. Decellularized, tissue-derived extracellular matrices (ECMs) have received attention for their potential use as tissue-engineered scaffolds. In considering meniscus-derived ECMs (mECMs) for meniscus tissue engineering, it is noteworthy that the inner and outer regions of the meniscus have different structural and biochemical features, potentially directing the differentiation of cells toward region-specific phenotypes...
November 28, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27884776/inverted-orientation-improves-decellularization-of-whole-porcine-hearts
#9
Po-Feng Lee, Eric Chau, Rafael Cabello, Alvin T Yeh, Luiz C Sampaio, Andrea S Gobin, Doris A Taylor
: In structurally heterogeneous organs, such as heart, it is challenging to retain extracellular matrix integrity in the thinnest regions (eg, valves) during perfusion decellularization and completely remove cellular debris from thicker areas. The high inflow rates necessary to maintain physiologic pressure can distend or damage thin tissues, but lower pressures prolong the process and increase the likelihood of contamination. We examined two novel retrograde decellularization methods for porcine hearts: inverting the heart or venting the apex to decrease inflow rate...
November 21, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27876676/anatomical-region-dependent-enhancement-of-3-dimensional-chondrogenic-differentiation-of-human-mesenchymal-stem-cells-by-soluble-meniscus-extracellular-matrix
#10
Benjamin B Rothrauff, Kazunori Shimomura, Riccardo Gottardi, Peter G Alexander, Rocky S Tuan
: Extracellular matrix (ECM) derived from decellularized tissues has been found to promote tissue neogenesis, most likely mediated by specific biochemical and physical signaling motifs that promote tissue-specific differentiation of progenitor cells. Decellularized ECM has been suggested to be efficacious for the repair of tissue injuries. However, decellularized meniscus contains a dense collagenous structure, which impedes cell seeding and infiltration and is not readily applicable for meniscus repair...
November 19, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27875930/hybrid-aorta-constructs-via-in-situ-crosslinking-of-poly-glycerol-sebacate-elastomer-within-a-decellularized-matrix
#11
Selcan Guler, Pezhman Hosseinian, Halil Murat Aydin
Decellularization of tissues and organs has high potential to obtain unique conformation and composition as native tissue structure but may result with weaken tissue mechanical strength. In this study poly(glycerol-sebacate) (PGS) elastomers were combined with decellularized aortae fragments to investigate the changes in mechanical properties. PGS prepolymer was synthesized via microwave irradiation then in-situ cross-linked within the decellularized aorta ECM. Tensile strength (σ) values were found comparable as 0...
November 22, 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27870078/cartilage-extracellular-matrix-as-a-biomaterial-for-cartilage-regeneration
#12
Emi A Kiyotake, Emily C Beck, Michael S Detamore
The extracellular matrix (ECM) of various tissues possesses the model characteristics that biomaterials for tissue engineering strive to mimic; however, owing to the intricate hierarchical nature of the ECM, it has yet to be fully characterized and synthetically fabricated. Cartilage repair remains a challenge because the intrinsic properties that enable its durability and long-lasting function also impede regeneration. In the last decade, cartilage ECM has emerged as a promising biomaterial for regenerating cartilage, partly because of its potentially chondroinductive nature...
November 2016: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/27866022/novel-approaches-toward-the-generation-of-bioscaffolds-as-a-potential-therapy-in-cardiovascular-tissue-engineering
#13
REVIEW
Fahimeh Shahabipour, Maciej Banach, Thomas P Johnston, Matteo Pirro, Amirhossein Sahebkar
Cardiovascular disease associated with myocardial infarction (MI) is among the leading causes of mortality worldwide, in part, due to the limited regenerative capacity of tissues. Although various approaches have been employed to generate bioartificial myocardial tissues, including surgical reconstruction and the use of biosynthetic or biological cell-free grafts, many challenges still remain. Natural biomaterials based on decellularization have made significant inroads into the development of favorable biomatrices for myocardial tissue regeneration...
November 9, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27863069/a-straightforward-method-to-produce-decellularized-dermis-based-matrices-for-tumor-cell-cultures
#14
Virginia Brancato, Maurizio Ventre, Giorgia Imparato, Francesco Urciuolo, Concetta Meo, Paolo A Netti
Decellularized matrices are steadily gaining popularity to study the biology of cells and tissues, since they represent a biomimetic environment in which cells can recapitulate certain behaviors that share similarities with those observed in vivo. Basically, biochemistry, microstructure and mechanics of the decellularized matrices are the most valuable properties that differentiate these culturing systems from conventional bidimensional models. Several procedures to decellularize tissues have been proposed so far, with the common aim to preserve the tissue chemical/physical properties of the original tissue...
November 11, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27862079/discarded-livers-find-a-new-life-engineered-liver-grafts-using-hepatocytes-recovered-from-marginal-livers
#15
Basak E Uygun, Maria-Louisa Izamis, Maria Jaramillo, Yibin Chen, Gavrielle Price, Sinan Ozer, Martin L Yarmush
Treatment for end-stage liver failure is restricted by the critical shortage of donor organs; about 4000 people die in the USA while waiting for a transplantable organ. This situation has been a major driving force behind the rise of tissue engineering to build artificial tissues/organs. Recent advancements in creating transplantable liver grafts using decellularized liver scaffolds bring the field closer to clinical translation. However, a source of readily available and highly functional adult hepatocytes in adequate numbers for regenerative liver therapies still remains unclear...
November 8, 2016: Artificial Organs
https://www.readbyqxmd.com/read/27858399/a-modified-chemical-protocol-of-decellularization-of-rat-sciatic-nerve-and-its-recellularization-with-mesenchimal-differentiated-schwann-like-cells-morphological-and-functional-assessments
#16
M M García-Pérez, H G Martínez-Rodríguez, G G López-Guerra, A Soto-Domínguez, S L Said-Fernández, R Morales-Avalos, R E Elizondo-Omaña, R Montes-de-Oca-Luna, S Guzmán-López, M L Castillo-Galván, O F Mendoza-Lemus, F Vílchez-Cavazos
The functional reconstruction of large neural defects usually requires the use of peripheral nerve autografts, though these have certain limitations. As a result, interest in new alternatives for autograft development has risen. The acellular peripheral nerve graft is an alternative for peripheral nerve injury repair, but to date there is not a standardized chemical decellularization method widely accepted. The objective of this study was to propose a modified chemical protocol of decellularization of rat sciatic nerve and its recellularization in vitro with mesenchimal differentiated Schwann-like cells...
November 18, 2016: Histology and Histopathology
https://www.readbyqxmd.com/read/27852013/modulation-of-cell-attachment-proliferation-and-angiogenesis-by-decellularized-dehydrated-human-amniotic-membrane-in-in-vitro-models
#17
Xuan Guo, Aleksandr Kaplunovsky, Raihana Zaka, Chuan Wang, Hemlata Rana, Jeffrey Turner, Qian Ye, Ivana Djuretic, Joseph Gleason, Vladimir Jankovic, Janice M Smiell, Mohit Bhatia, Wolfgang Hofgartner, Robert Hariri
BACKGROUND: Decellularized, dehydrated human amniotic membrane (DDHAM) is an extracellular matrix devoid of cells, cell debris, and growth factors. This study examines the effect of cell attachment to the DDHAM and the induced cellular responses. MATERIALS AND METHODS: The cell types employed in this study were human dermal fibroblasts (HDF), human epithelial keratinocytes (HEK), and human dermal microvascular endothelial cells (HDMEC), all of which play critical roles in the wound healing process...
October 24, 2016: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/27846786/systematic-comparison-of-protocols-for-the-preparation-of-human-articular-cartilage-for-use-as-scaffold-material-in-cartilage-tissue-engineering
#18
Cornelia Schneider, Johannes Lehmann, Gerjo van Osch, Florian Hildner, Andreas Herbert Teuschl, Xavier Monforte, David Miosga, Patrick Heimel, Eleni Priglinger, Heinz Redl, Susanne Wolbank, Sylvia Nürnberger
Natural extracellular matrix (ECM) derived biomaterials from decellularized allogenic tissues are of increasing interest for tissue engineering since their structure and composition provide a complexity that is not achievable with current manufacturing techniques. The prerequisite to bring allogenic tissue from bench to bedside as a functional biomaterial is the full removal of cells while preserving most of its native characteristics such as structure and composition. The exceptionally dense structure of articular cartilage, however, poses a special challenge for decellularisation, scaffold preparation and reseeding...
November 15, 2016: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/27845391/effect-of-bovine-pericardial-extracellular-matrix-scaffold-niche-on-seeded-human-mesenchymal-stem-cell-function
#19
Zhi Zhao Liu, Maelene L Wong, Leigh G Griffiths
Numerous studies have focused on generation of unfixed bovine pericardium (BP) extracellular matrix (ECM) for clinical application. However, the extent to which maintenance of native ECM niche is capable of directing behavior of repopulating cells remains relatively unexplored. By exploiting the sidedness of BP scaffolds (i.e., serous or fibrous surface), this study aims to determine the effect of ECM niche preservation on cellular repopulation using different scaffold generation methods. BP underwent either sodium dodecyl sulfate (SDS) decellularization or stepwise, solubilization-based antigen removal using amidosulfobetaine-14 (ASB-14)...
November 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27830445/tissue-engineering-for-neurodegenerative-diseases-using-human-amniotic-membrane-and-umbilical-cord
#20
Anahí Sanluis-Verdes, Namibia Sanluis-Verdes, María Jesús Manso-Revilla, Antonio Manuel Castro-Castro, Jorge Pombo-Otero, María Fraga-Mariño, Jacinto Sanchez-Ibañez, Nieves Doménech, María Esther Rendal-Vázquez
Regenerative medicine, based on the use of stem cells, scaffolds and growth factors, has the potential to be a good approach for restoring damaged tissues of the central nervous system. This study investigated the use of human amniotic mesenchymal stem cells (hAMSC), human amniotic epithelial stem cells (hAESC), and human Wharton's jelly mesenchymal stem cells (hWJMSC) derived from human umbilical cord as a source of stem cells, and the potential of the human amniotic membrane (HAM) as a scaffold and/or source of growth factors to promote nerve regeneration...
November 9, 2016: Cell and Tissue Banking
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