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Decellularized matrix

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https://www.readbyqxmd.com/read/29472603/bronchial-extracellular-matrix-from-copd-patients-induces-altered-gene-expression-in-repopulated-primary-human-bronchial-epithelial-cells
#1
Ulf Hedström, Oskar Hallgren, Lisa Öberg, Amy DeMicco, Outi Vaarala, Gunilla Westergren-Thorsson, Xiaohong Zhou
Chronic obstructive pulmonary disease (COPD) is a serious global health problem characterized by chronic airway inflammation, progressive airflow limitation and destruction of lung parenchyma. Remodeling of the bronchial airways in COPD includes changes in both the bronchial epithelium and the subepithelial extracellular matrix (ECM). To explore the impact of an aberrant ECM on epithelial cell phenotype in COPD we developed a new ex vivo model, in which normal human bronchial epithelial (NHBE) cells repopulate and differentiate on decellularized human bronchial scaffolds derived from COPD patients and healthy individuals...
February 22, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29469614/tgf-%C3%AE-1-induced-deposition-of-provisional-extracellular-matrix-by-tracheal-basal-cells-promotes-epithelial-to-mesenchymal-transition-in-a-jnk1-dependent-manner
#2
Jos L van der Velden, Darcy E Wagner, Karolyn G Lahue, Sarah T Abdalla, Ying W Lam, Daniel J Weiss, Yvonne M W Janssen-Heininger
Epithelial cells have been suggested as potential drivers of lung fibrosis, although the epithelial-dependent pathways that promote fibrogenesis remain unknown. Extracellular matrix is increasingly recognized as an environment that can drive cellular responses in various pulmonary diseases. In this study, we demonstrate that transforming growth factor- beta (TGF-β1)-stimulated mouse tracheal basal (MTB) cells produce provisional matrix proteins in vitro, which initiate mesenchymal changes in subsequently freshly plated MTB cells via Rho kinase- and c-Jun-N-terminal kinase (JNK1)-dependent processes...
February 22, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29459044/decellularized-human-fetal-intestine-as-a-bioscaffold-for-regeneration-of-the-rabbit-bladder-submucosa
#3
Abdol-Mohammad Kajbafzadeh, Reza Khorramirouz, Ahmad Masoumi, Sorena Keihani, Behnam Nabavizadeh
PURPOSE: We aim to report a method to create a natural acellular scaffold from human fetal small intestine for augmentation cystoplasty in rabbits. METHODS: Fetal intestines were decellularized by immersion in a hypotonic solution. The success of this protocol was evaluated by histological analysis, scanning electron microscopy and measurement of collagen and sulfated glycosaminoglycan of the acellular tissues. Eight mature rabbits were selected and acellular scaffolds were implanted on the exposed urothelium...
February 1, 2018: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29452238/human-adipose-tissue-derived-stromal-cells-in-combination-with-exogenous-stimuli-facilitate-three-dimensional-network-formation-of-human-endothelial-cells-derived-from-various-sources
#4
Dominique Manikowski, Birgit Andrée, Esther Samper, Clémence Saint-Marc, Ruth Olmer, Peter Vogt, Sarah Strauß, Axel Haverich, Andres Hilfiker
In natural tissues, the nutrition of cells and removal of waste products is facilitated by a dense capillary network which is generated during development. This perfusion system is also indispensable for tissue formation in vitro. Nutrition depending solely on diffusion is not sufficient to generate tissues of clinically relevant dimensions, which is a core aim in tissue engineering research. In this study, the establishment of a vascular network was investigated in a self-assembling approach employing endothelial and mural cells...
February 13, 2018: Vascular Pharmacology
https://www.readbyqxmd.com/read/29450424/cd44v6-increases-gastric-cancer-malignant-phenotype-by-modulating-adipose-stromal-cell-mediated-ecm-remodeling
#5
Bianca N Lourenço, Nora L Springer, Daniel Ferreira, Carla Oliveira, Pedro L Granja, Claudia Fischbach
CD44, an abundantly expressed adhesion molecule, and its alternative splice variants have been associated with tumorigenesis and metastasis. In the context of gastric cancer (GC), de novo expression of CD44 variant 6 (CD44v6) is found in more than 60% of GCs, but its role in the pathogenesis and progression of this type of cancer remains unclear. Using a combination of media conditioning experiments and decellularized extracellular matrices (ECMs), this study investigates the hypothesis that CD44v6 overexpression enhances tumor cell malignant behavior by modulating stromal cell-mediated ECM remodeling...
February 16, 2018: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/29446183/comparison-of-detergent-based-decellularization-protocols-for-the-removal-of-antigenic-cellular-components-in-porcine-aortic-valve
#6
Xiaohong Liu, Ning Li, Dejun Gong, Cuiping Xia, Zhiyun Xu
BACKGROUND: Various detergent-based protocols are used to remove cells and cellular debris in porcine aortic valve (PAV). However, the removal of antigenic cellular components has not been thoroughly elucidated to date. In this study, we used 4 detergent-based protocols to decellularize PAVs and aimed to evaluate their effects on removing antigenic cellular components. METHODS: Porcine aortic valves were decellularized using sodium dodecyl sulfate (SDS), SDS in combination with sodium deoxycholate (SDS/SD), Triton X-100, and Triton X-100 in combination with SD (Triton X-100/SD), respectively...
February 15, 2018: Xenotransplantation
https://www.readbyqxmd.com/read/29443081/recombinant-collagen-i-peptide-microcarriers-for-cell-expansion-and-their-potential-use-as-cell-delivery-system-in-a-bioreactor-model
#7
Melva Suarez Muñoz, Davide Confalonieri, Heike Walles, Elisabeth M W M van Dongen, Gudrun Dandekar
Tissue engineering is a promising field, focused on developing solutions for the increasing demand on tissues and organs regarding transplantation purposes. The process to generate such tissues is complex, and includes an appropriate combination of specific cell types, scaffolds, and physical or biochemical stimuli to guide cell growth and differentiation. Microcarriers represent an appealing tool to expand cells in a three-dimensional (3D) microenvironment, since they provide higher surface-to volume ratios and mimic more closely the in vivo situation compared to traditional two-dimensional methods...
February 7, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29440901/guided-osteoporotic-bone-regeneration-with-composite-scaffolds-of-mineralized-ecm-heparin-membrane-loaded-with-bmp2-related-peptide
#8
Tingfang Sun, Man Liu, Sheng Yao, Yanhui Ji, Lei Shi, Kai Tang, Zekang Xiong, Fan Yang, Kaifang Chen, Xiaodong Guo
Introduction: At present, the treatment of osteoporotic defects poses a great challenge to clinicians, owing to the lower regeneration capacity of the osteoporotic bone as compared with the normal bone. The guided bone regeneration (GBR) technology provides a promising strategy to cure osteoporotic defects using bioactive membranes. The decellularized matrix from the small intestinal submucosa (SIS) has gained popularity for its natural microenvironment, which induces cell response. Materials and methods: In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29440894/sustained-release-of-fgf-2-from-a-hybrid-hydrogel-of-heparin-poloxamer-and-decellular-matrix-promotes-the-neuroprotective-effects-of-proteins-after-spinal-injury
#9
He-Lin Xu, Fu-Rong Tian, Jian Xiao, Pian-Pian Chen, Jie Xu, Zi-Liang Fan, Jing-Jing Yang, Cui-Tao Lu, Ying-Zheng Zhao
Introduction: The short lifetime of protein-based therapies has largely limited their therapeutic efficacy in injured nervous post-spinal cord injury (post-SCI). Methods: In this study, an affinity-based hydrogel delivery system provided sustained-release of proteins, thereby extending the efficacy of such therapies. The affinity-based hydrogel was constructed using a novel polymer, heparin-poloxamer (HP), as a temperature-sensitive bulk matrix and decellular spinal cord extracellular matrix (dscECM) as an affinity depot of drug...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29431032/decellularized-muscle-supports-new-muscle-fibers-and-improves-function-following-volumetric-injury
#10
Michael James McClure, David Josh Cohen, Allison N Ramey, Caroline B Bivens, Satya Mallu, Jonathan E Isaacs, Emily Imming, Yen-Chen Huang, MoonHae Sunwoo, Zvi Schwartz, Barbara Boyan
Current strategies to treat volumetric muscle loss use primarily pedicle or free muscle transfers, but these grafts fail to adequately regenerate functional tissue. Decellularized soft tissue grafts possess physical and chemical cues to promote muscle regeneration, suggesting their potential for use in large muscle defects. In this study, we developed a decellularized muscle matrix (DMM) graft using rat gastrocnemius. Anisotropy and chemical components of the extracellular matrix were retained, including laminin, fibronectin, and collagen...
February 12, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29411714/effects-of-tissue-processing-on-bioactivity-of-cartilage-matrix-based-hydrogels-encapsulating-osteoconductive-particles
#11
Jakob M Townsend, Taylor A Zabel, Yi Feng, Jinxi Wang, Brian T Andrews, Randy Nudo, Cory Berkland, Michael S Detamore
In the treatment of severe traumatic brain injury (TBI), decompressive craniectomy is commonly used to remove a large portion of calvarial bone to allow unimpeded brain swelling. Hydrogels have the potential to revolutionize TBI treatment by permitting a single-surgical intervention, remaining pliable during brain swelling, and tuned to regenerate bone after swelling has subsided. With this motivation, our goal is to present a pliable material capable of regenerating calvarial bone across a critical size defect...
February 7, 2018: Biomedical Materials
https://www.readbyqxmd.com/read/29407754/assessment-of-static-and-perfusion-methods-for-decellularization-of-pcl-membrane-supported-periodontal-ligament-cell-sheet-constructs
#12
Amro Farag, Saeed M Hashimi, Cedryck Vaquette, Fabio Z Volpato, Dietmar W Hutmacher, Saso Ivanovski
OBJECTIVES: Decellularization aims to harness the regenerative properties of native extracellular matrix. The objective of this study was to evaluate different methods of decellularization of periodontal ligament cell sheets whilst maintaining their structural and biological integrity. DESIGN: Human periodontal ligament cell sheets were placed onto melt electrospun polycaprolactone (PCL) membranes that reinforced the cell sheets during the various decellularization protocols...
January 31, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29402858/skeletal-extracellular-matrix-supports-cardiac-differentiation-of-embryonic-stem-cells-a-potential-scaffold-for-engineered-cardiac-tissue
#13
Xian Hong, Yin Yuan, Xiaoxi Sun, Meiling Zhou, Guangyu Guo, Quan Zhang, Jürgen Hescheler, Jiaoya Xi
BACKGROUND/AIMS: Decellularized cardiac extracellular matrix (cECM) has been widely considered as an attractive scaffold for engineered cardiac tissue (ECT), however, its application is limited by immunogenicity and shortage of organ donation. Skeletal ECM (sECM) is readily available and shows similarities with cECM. Here we hypothesized that sECM might be an alternative scaffold for ECT strategies. METHODS: Murine ventricular tissue and anterior tibial muscles were sectioned into 300 mm-thick, and then cECM and sECM were acquired by pretreatment/SDS/TritonX-100 three-step-method...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29394158/microspheres-containing-decellularized-cartilage-induce-chondrogenesis-in-vitro-and-remain-functional-after-incorporation-within-a-poly-caprolactone-filament-useful-for-fabricating-a-3d-scaffold
#14
Paulomi Ghosh, Stacey M S Gruber, Chia-Ying Lin, Patrick W Whitlock
In this study, articular cartilage was decellularized preserving a majority of the inherent proteins, cytokines, growth factors and sGAGs. The decellularized cartilage matrix (dCM) was then encapsulated in poly(lactic acid) microspheres (MS + dCM) via double emulsion. Blank microspheres without dCM, MS(-), were also produced. The microspheres were spherical in shape and protein encapsulation efficiency within MS + dCM was 63.4%. The sustained release of proteins from MS + dCM was observed over 4 weeks in vitro...
February 2, 2018: Biofabrication
https://www.readbyqxmd.com/read/29392581/a-novel-patient-derived-xenograft-model-for-claudin-low-triple-negative-breast-cancer
#15
Margarite D Matossian, Hope E Burks, Annie C Bowles, Steven Elliott, Van T Hoang, Rachel A Sabol, Nicholas C Pashos, Benjamen O'Donnell, Kristin S Miller, Bahia M Wahba, Bruce A Bunnell, Krzysztof Moroz, Arnold H Zea, Steven D Jones, Augusto C Ochoa, Amir A Al-Khami, Fokhrul Hossain, Adam I Riker, Lyndsay V Rhodes, Elizabeth C Martin, Lucio Miele, Matthew E Burow, Bridgette M Collins-Burow
BACKGROUND: Triple-negative breast cancer (TNBC) subtypes are clinically aggressive and cannot be treated with targeted therapeutics commonly used in other breast cancer subtypes. The claudin-low (CL) molecular subtype of TNBC has high rates of metastases, chemoresistance and recurrence. There exists an urgent need to identify novel therapeutic targets in TNBC; however, existing models utilized in target discovery research are limited. Patient-derived xenograft (PDX) models have emerged as superior models for target discovery experiments because they recapitulate features of patient tumors that are limited by cell-line derived xenograft methods...
February 1, 2018: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29391037/effect-of-pulsatile-flow-perfusion-on-decellularization
#16
Sung Min Park, Seran Yang, Se-Min Rye, Seong Wook Choi
BACKGROUND: Decellularized animal organs have been used as scaffolds for tissue engineering. To make a properly functioning scaffolds, the extracellular matrix (ECM) components must be preserved after decellularization. Because pulsatile flow is known to be beneficial for tissue perfusion, pulsatile perfusion of a detergent might decrease the exposure time of the tissues to the detergent used for decellularization. Using Energy Equivalent Pressure (EEP) as a pulsatility parameter, the effect of pulsatile flow in decellularization process is studied...
February 1, 2018: Biomedical Engineering Online
https://www.readbyqxmd.com/read/29390876/decellularization-and-solubilization-of-porcine-liver-for-use-as-a-substrate-for-porcine-hepatocyte-culture-method-optimization-and-comparison
#17
Ramon E Coronado, Maria Somaraki-Cormier, Shanmugasundaram Natesan, Robert J Christy, Joo L Ong, Glenn A Halff
Biologic substrates, prepared by decellularizing and solubilizing tissues, have been of great interest in the tissue engineering field because of the preservation of complex biochemical constituents found in the native extracellular matrix (ECM). The integrity of the ECM is critical for cell behavior, adhesion, migration, differentiation, and proliferation that in turn affect homeostasis and tissue regeneration. Previous studies have shown that various processing methods have a distinctive way of affecting the composition of the decellularized ECM...
December 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29377912/recellularization-of-rat-liver-an-in-vitro-model-for-assessing-human-drug-metabolism-and-liver-biology
#18
Matthew J Robertson, Benjamin Soibam, Jacqueline G O'Leary, Luiz C Sampaio, Doris A Taylor
Liver-like organoids that recapitulate the complex functions of the whole liver by combining cells, scaffolds, and mechanical or chemical cues are becoming important models for studying liver biology and drug metabolism. The advantages of growing cells in three-dimensional constructs include enhanced cell-cell and cell-extracellular matrix interactions and preserved cellular phenotype including, prevention of de-differentiation. In the current study, biomimetic liver constructs were made via perfusion decellularization of rat liver, with the goal of maintaining the native composition and structure of the extracellular matrix...
2018: PloS One
https://www.readbyqxmd.com/read/29370745/design-and-fabrication-of-injectable-microcarriers-composed-of-acellular-cartilage-matrix-and-chitosan
#19
Farzane Sivandzade, Shohreh Mashayekhan
Cartilage is an avascular tissue with limited self-repair ability. Since the methods for treatment of cartilage defects have not been effective, new therapies based on tissue engineering are considered over the recent years. In this study, human cartilage tissue was decellularized and porous injectable microcarriers (MCs) composed of acellularextracellular matrix (ECM) of cartilage tissue and chitosan(CS), with different ECM weight ratios, were fabricated by electrosprayingtechnique to be used in the treatment of articular cartilage defects...
January 26, 2018: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29367647/proteomic-analysis-of-nucleus-pulposus-cell-derived-extracellular-matrix-niche-and-its-effect-on-phenotypic-alteration-of-dermal-fibroblasts
#20
Minting Yuan, Pei-Jing Pai, Xiaofen Liu, Henry Lam, Barbara P Chan
Reconstituting biomimetic matrix niche in vitro and culturing cells at the cell niche interface is necessary to understand the effect and function of the specific matrix niche. Here we attempted to reconstitute a biomimetic extracellular matrix (ECM) niche by culturing nucleus pulposus cells (NPCs) in a collagen microsphere system previously established and allowing them to remodel the template matrix. The reconstituted NPC-derived complex ECM was obtained after decellularization and the composition of such niche was evaluated by proteomic analysis...
January 24, 2018: Scientific Reports
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