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Decellularized matrix

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https://www.readbyqxmd.com/read/29222839/decellularized-human-liver-extracellular-matrix-hdlm-mediated-hepatic-differentiation-of-human-induced-pluripotent-stem-cells-hipsc
#1
Maria Jaramillo, Heidi Yeh, Martin L Yarmush, Basak E Uygun
Liver tissue engineering has emerged as a promising approach in organ transplantation, but has been hampered by the lack of a reliable and readily available cell source. Induced pluripotent cells (hiPSC) have been highlighted a desirable source, due to their differentiation potential, ability to self-renew and the possibility of making patient specific cells. We developed a decellularization protocol that efficiently removes cellular material, while retaining extracellular matrix components. Subsequently, hiPSC were differentiated on the decellularized human liver matrix (hDLM) scaffolds using an established hepatic differentiation protocol...
December 8, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29221151/collagen-i-promotes-hepatocellular-carcinoma-cell-proliferation-by-regulating-integrin-%C3%AE-1-fak-signaling-pathway-in-nonalcoholic-fatty-liver
#2
Xinglong Zheng, Wenyan Liu, Junxi Xiang, Peng Liu, Mengyun Ke, Bo Wang, Rongqian Wu, Yi Lv
Nonalcoholic fatty liver disease (NAFLD) has become a major risk factor for hepatocellular carcinoma (HCC) worldwide. However, the underlying mechanism remains insufficiently elucidated. The expression of Collagen I, an important component of extracellular matrix (ECM), was increased during the progression from simple steatosis to NASH. The purpose of this study was to investigate the role of Collagen I in NAFLD-related HCC. To study this, the decellularized liver matrix, which preserves the pathological changes of ECM, was prepared from the human fatty liver (FLM) and human normal liver (NLM)...
November 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29211918/decellularized-human-placenta-supports-hepatic-tissue-and-allows-rescue-in-acute-liver-failure
#3
Zurab Kakabadze, Anne Kakabadze, David Chakhunashvili, Lika Karalashvili, Ekaterine Berishvili, Yogeshwar Sharma, Sanjeev Gupta
Tissue engineering with scaffolds to form transplantable organs is of wide interest. Decellularized tissues have been tested for this purpose, although supplies of healthy donor tissues, vascular recellularization for perfusion and tissue homeostasis in engineered organs pose challenges. We hypothesized decellularized human placenta will be suitable for tissue engineering. The universal availability and unique structures of placenta for accommodating tissue, including presence of embedded vessels, were major attractions...
December 6, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29210278/decellularized-liver-matrix-modified-cryogel-scaffolds-as-potential-hepatocyte-carriers-in-bioartificial-liver-support-systems-and-implantable-liver-constructs
#4
Apeksha Damania, Anupam Kumar, Arun Kumar Teotia, Kimura Haruna, Masamichi Kamihira, Hiroyuki Ijima, Shiv Kumar Sarin, Ashok Kumar
Recent progress in the use of decellularized organ scaffolds as regenerative matrices for tissue engineering holds great promise in addressing the issue of donor organ shortage. Decellularization preserves the mechanical integrity, composition, and microvasculature critical for zonation of hepatocytes in the liver. Earlier studies have reported the possibility of repopulating decellularized matrices with hepatic cell lines or stem cells to improve liver regeneration. In this work, we study the versatility of decellularized liver matrix as a substrate coating of 3D cryogel scaffolds...
December 6, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29209868/laser-surface-modification-of-decellularized-extracellular-cartilage-matrix-for-cartilage-tissue-engineering
#5
Eva Goldberg-Bockhorn, Silke Schwarz, Rachana Subedi, Alexander Elsässer, Ricarda Riepl, Paul Walther, Ludwig Körber, Roman Breiter, Karl Stock, Nicole Rotter
The implantation of autologous cartilage as the gold standard operative procedure for the reconstruction of cartilage defects in the head and neck region unfortunately implicates a variety of negative effects at the donor site. Tissue-engineered cartilage appears to be a promising alternative. However, due to the complex requirements, the optimal material is yet to be determined. As demonstrated previously, decellularized porcine cartilage (DECM) might be a good option to engineer vital cartilage. As the dense structure of DECM limits cellular infiltration, we investigated surface modifications of the scaffolds by carbon dioxide (CO2) and Er:YAG laser application to facilitate the migration of chondrocytes inside the scaffold...
December 6, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/29196674/artificial-controlled-model-of-blood-circulation-system-for-adhesive-evaluation
#6
Sang-Myung Jung, Goo Yong Chung, Hwa Sung Shin
Since there are several casualties due to uncontrolled bleeding resulting from simple injury to surgery, effective styptic or vessel adhesives are important; however, their development is limited by the lack of standardized systems to evaluate potential compounds. The current study outlines the development of an aorta styptic evaluation system, comprising of decellularized swine aorta tissue and a heart pump-mimicking system. Although the cells in the swine aorta were removed, the structural stability of the aorta was sustained due to the maintenance of the extracellular matrix...
December 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29192810/histological-and-ultrastructural-evaluation-of-human-decellularized-matrix-as-a-hernia-repair-device
#7
Martina Ghetti, Valentina Papa, Giovanni Deluca, Valeria Purpura, Paolo Ruscelli, Davide Melandri, Daniela Capirossi, Evandro Nigrisoli, Paola Minghetti, Elena Bondioli, Giovanna Cenacchi
Recently, interest has been increasing for human decellularized matrices, due to their ability to reduce numerous side effects related to hernia repair. To date, only animal studies investigated the biological interaction post-implant of human decellularized matrices for soft tissue repair. Therefore, the aim of this study was to evaluate the morphological response one year post implant of human decellularized matrix, through morphological analysis of human biopsies. The histological and ultrastructural results revealed a perfect cellular repopulation and neoangiogenesis, with minimal inflammatory response and a well-organized collagen matrix...
December 1, 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29191508/in-vivo-performance-of-freeze-dried-decellularized-pulmonary-heart-valve-allo-and-xenografts-orthotopically-implanted-into-juvenile-sheep
#8
Tobias Goecke, Karolina Theodoridis, Igor Tudorache, Anatol Ciubotaru, Serghei Cebotari, Robert Ramm, Klaus Höffler, Samir Sarikouch, Andrés Vásquez-Rivera, Axel Haverich, Willem F Wolkers, Andres Hilfiker
The decellularization of biological tissues decreases immunogenicity, allows repopulation with cells, and may lead to improved long-term performance after implantation. Freeze drying these tissues would ensure off-the-shelf availability, save storage costs, and facilitates easy transport. This study evaluates the in vivo performance of freeze-dried decellularized heart valves in juvenile sheep. TritonX-100 and sodium dodecylsulfate decellularized ovine and porcine pulmonary valves (PV) were freeze-dried in a lyoprotectant sucrose solution...
November 27, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29187125/nitro-oleic-acid-no2oa-release-enhances-regional-angiogenesis-in-a-rat-abdominal-wall-defect-model
#9
Antonio D'Amore, Marco Fazzari, Hongbin Jiang, Samuel K Luketich, Michael E Luketich, Richard F Hoff, Daniel L Jacobs, Xinzhu Gu, Stephen F Badylak, Bruce A Freeman, William R Wagner
Ventral hernia is often addressed surgically by the placement of prosthetic materials, either synthetic or from allogeneic and xenogeneic biologic sources. Despite advances in surgical approaches and device design, a number of postsurgical limitations remain, including hernia recurrence, mesh encapsulation, and reduced vascularity of the implanted volume. The in situ controlled release of angiogenic factors from a scaffold facilitating abdominal wall repair might address some of these issues associated with sub-optimal tissue reconstruction...
November 29, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29185592/decellularization-of-placentas-establishing-a-protocol
#10
L C P C Leonel, C M F C Miranda, T M Coelho, G A S Ferreira, R R Caãada, M A Miglino, S E Lobo
Biological biomaterials for tissue engineering purposes can be produced through tissue and/or organ decellularization. The remaining extracellular matrix (ECM) must be acellular and preserve its proteins and physical features. Placentas are organs of great interest because they are discarded after birth and present large amounts of ECM. Protocols for decellularization are tissue-specific and have not been established for canine placentas yet. This study aimed at analyzing a favorable method for decellularization of maternal and fetal portions of canine placentas...
November 17, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29183849/a-sterilization-method-for-decellularized-xenogeneic-cardiovascular-scaffolds
#11
Cátia Fidalgo, Laura Iop, Manuela Sciro, Michael Harder, Dimosthenis Mavrilas, Sotirios Korossis, Andrea Bagno, Giorgio Palù, Paola Aguiari, Gino Gerosa
Decellularized xenogeneic scaffolds have shown promise to be employed as compatible and functional cardiovascular biomaterials. However, one of the main barriers to their clinical exploitation is the lack of appropriate sterilization procedures. This study investigated the efficiency of a two-step sterilization method, antibiotics/antimycotic (AA) cocktail and peracetic acid (PAA), on porcine and bovine decellularized pericardium. In order to assess the efficiency of the method, a sterilization assessment protocol was specifically designed, comprising: i) controlled contamination with a known amount of bacteria; ii) sterility test; iii) identification of contaminants through MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) mass spectrometry and iv) quantification by the Most Probable Number (MPN) method...
November 25, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29167844/recellularization-of-decellularized-adipose-tissue-derived-stem-cells-role-of-the-cell-secreted-extracellular-matrix-in-cellular-differentiation
#12
V Guneta, Z Zhou, N S Tan, S Sugii, M T C Wong, C Choong
Adipose-derived stem cells (ASCs) are found in a location within the adipose tissue known as the stem cell niche. The ASCs in the niche are maintained in the quiescent state, and upon exposure to various microenvironmental triggers are prompted to undergo proliferation or differentiation. These microenvironmental triggers also modulate the extracellular matrix (ECM), which interacts with the cells through the cytoskeleton and induces downstream events inside the cells that bring about a change in cell behaviour...
November 23, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/29164819/decellularized-bovine-cotyledons-may-serve-as-biological-scaffolds-with-preserved-vascular-arrangement
#13
Rodrigo da Silva Nunes Barreto, Patricia Romagnolli, Andrea Maria Mess, Maria Angelica Miglino
Technically produced scaffolds are common to establish transplantable tissues for Regenerative Medicine, but also biological ones that are closer to the natural condition become of interest. Placentas are promising, because they represented available, complete organs with rich extracellular matrix (ECM) and well developed vasculature that easily could build anastomoses to a host´s organ. Only placentas from larger animal models such as the bovine meet the dimensions large enough for most organs, but are not adequately described yet...
November 21, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29159728/in-lab-manufacturing-of-decellularized-rat-renal-scaffold-for-kidney-bioengineering
#14
Andrea Peloso, Antonio Citro, Valeria Corradetti, Szandra Brambilla, Graziano Oldani, Francesco Calabrese, Tommaso Dominioni, Marcello Maestri, Lorenzo Cobianchi
Whole-organ decellularization is recently gaining interest in the transplantation field as strategy to obtain acellular scaffold only composed by extracellular matrix. These structures, that still remain organ-specific in terms of biological cues and tridimensional morphology could be then recellularized with patient's autologous cells. The final result should be a nwe transplantable autologous organ that should by-pass, at the same time, the problem of organ shortage and secondly the consequences related to the immunosuppression need...
November 21, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29157906/novel-decellularized-liver-matrix-alginate-hybrid-gel-beads-for-the-3d-culture-of-hepatocellular-carcinoma-cells
#15
Dongsheng Sun, Yang Liu, Huihui Wang, Fei Deng, Ying Zhang, Shan Zhao, Xiaojun Ma, Huijian Wu, Guangwei Sun
Developing reliable three-dimensional (3D) cell culture systems that can mimic native tumor microenvironments is necessary for investigating the mechanism of hepatocellular carcinoma (HCC) metastasis and screen therapeutic drugs. In the present study, we developed decellularized liver matrix-alginate (DLM-ALG) hybrid gel beads. DLM powder was prepared by optimized decellularization methods and liquid nitrogen grinding. DLM-ALG beads were generated by dropping alginate solution containing DLM powder into a gelling bath...
November 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29156171/the-use-of-decellularized-human-placenta-in-full-thickness-wound-repair-and%C3%A2-periarticular-soft-tissue-reconstruction-an-update-on-regenerative-healing
#16
REVIEW
Stephen A Brigido, Scott C Carrington, Nicole M Protzman
Prolonged or incomplete healing of the foot and ankle can pose significant challenges. Therefore, investigators have begun searching for alternative treatment strategies. With advances in tissue engineering, decellularized human placental connective tissue matrix has been suggested as a means to achieve more rapid and complete healing for various soft tissue and bone procedures. Basic science and clinical studies have shown that decellularized human placental connective tissue matrix can support regenerative healing through cellular migration, accelerated tissue remodeling, and the establishment of functional tissue...
January 2018: Clinics in Podiatric Medicine and Surgery
https://www.readbyqxmd.com/read/29155942/detergent-based-decellularization-strategy-preserves-macro-and-microstructure-of-heart-valves
#17
Jessica Haupt, Georg Lutter, Stanislav N Gorb, Dan T Simionescu, Derk Frank, Jette Seiler, Alina Paur, Irma Haben
OBJECTIVES: Biological tissue has great potential to function as bioprostheses in patients for heart valve replacement. As these matrices are mainly xenogenic, the immunogenicity needs to be reduced by decellularization steps. Reseeding of bioscaffolds has tremendous potential to prevent calcification upon implantation, so intact microstructure of the material is mandatory. An optimal decellularization protocol of heart valves resulting in adequate preservation of the extracellular architecture has still not been developed...
October 9, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29131298/decellularized-kidney-matrix-as-functional-material-for-whole-organ-tissue-engineering
#18
Marina Figliuzzi, Barbara Bonandrini, Andrea Remuzzi
Renal transplantation is currently the most effective treatment for end-stage renal disease, which represents one of the major current public health problems. However, the number of available donor kidneys is drastically insufficient to meet the demand, causing prolonged waiting lists. For this reason, tissue engineering offers great potential to increase the pool of donated organs for kidney transplantation, by way of seeding cells on supporting scaffolding material. Biological scaffolds are prepared by removing cellular components from the donor organs using a decellularization process with detergents, enzymes or other cell lysing solutions...
November 11, 2017: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/29128541/a-pegylated-platelet-free-plasma-hydrogel-based-composite-scaffold-enables-stable-vascularization-and-targeted-cell-delivery-for-volumetric-muscle-loss
#19
Amit Aurora, Nicole Wrice, Thomas J Walters, Robert J Christy, Shanmugasundaram Natesan
Extracellular matrix (ECM) scaffolds are being used for the clinical repair of soft tissue injuries. Although improved functional outcomes have been reported, ECM scaffolds show limited tissue specific remodeling response with concomitant deposition of fibrotic tissue. One plausible explanation is the regression of blood vessels which may be limiting the diffusion of oxygen and nutrients across the scaffold. Herein we develop a composite scaffold as a vasculo-inductive platform by integrating PEGylated platelet free plasma (PFP) hydrogel with a muscle derived ECM scaffold (m-ECM)...
November 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29127860/human-embryonic-stem-cell-derived-cardiovascular-progenitor-cells-efficiently-colonize-in-bfgf-tethered-natural-matrix-to-construct-contracting-humanized-rat-hearts
#20
Sarah Rajabi, Sara Pahlavan, Mohammad Kazemi Ashtiani, Hassan Ansari, Saeed Abbasalizadeh, Forough Azam Sayahpour, Fahimeh Varzideh, Sawa Kostin, Nasser Aghdami, Thomas Braun, Hossein Baharvand
Bioengineering of whole hearts using human embryonic stem cells (hESCs)-derived cardiovascular progenitor cells (CPCs) and natural matrices is a promising approach to overcome organ donor shortage threatening millions of patients awaiting for heart transplantation. Here, we developed a novel strategy for generation of heart constructs by repopulating engineered decellularized rat hearts using hESCs-derived CPCs. Careful expansion of CPCs in a scalable stirred-suspension bioreactor combined with step-wise seeding (60 million cells in 3 steps of 20 million per 1...
November 1, 2017: Biomaterials
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