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Decellularized matrix

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https://www.readbyqxmd.com/read/28433789/macromolecular-crowding-for-tailoring-tissue-derived-fibrillated-matrices
#1
Valentina Magno, Jens Friedrichs, Heather M Weber, Marina C Prewitz, Mikhail V Tsurkan, Carsten Werner
Tissue-derived fibrillated matrices can be instrumental for the in vitro reconstitution of multiphasic extracellular microenvironments. However, despite of several advantages, the obtained scaffolds so far offer a rather narrow range of materials characteristics only. In this work, we demonstrate how macromolecular crowding (MMC) - the supplementation of matrix reconstitution media with synthetic or natural macromolecules in ways to create excluded volume effects (EVE) - can be employed for tailoring important structural and biophysical characteristics of kidney-derived fibrillated matrices...
April 19, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28432818/characterization-of-decellularized-ovine-small-intestine-submucosal-layer-as-extracellular-matrix-based-scaffold-for-tissue-engineering
#2
Morteza Rashtbar, Jamshid Hadjati, Jafar Ai, Issa Jahanzad, Mahmoud Azami, Sadegh Shirian, Somayeh Ebrahimi-Barough, Esmaeil Sadroddiny
Extracellular matrix-based scaffolds derived from mammalian tissues have been used in tissue engineering applications. Among all the tissues, decellularized small intestine submucosal layer (SIS) has been recently investigated for its exceptional characteristics and biocompatibilities. These investigations have been mainly focused on the decellularized porcine SIS; however, there has not been any report on ovine SIS (OSIS) layer. In this study, OSIS was decellularized and its physical, chemical, and morphological properties were evaluated...
April 22, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28430301/towards-a-bioengineered-kidney-recellularization-strategies-for-decellularized-native-kidney-scaffolds
#3
Michele Fedecostante, Oana G Onciu, Koen G C Westphal, Rosalinde Masereeuw
Patients with end-stage renal disease often undergo dialysis as a partial substitute for kidney function while waiting for their only treatment option: a kidney transplant. Several research directions emerged for alternatives in support of the ever-growing numbers of patients. Recent years brought big steps forward in the field, with researchers questioning and improving the current dialysis devices as well as moving towards the design of a bioengineered kidney. Whole-organ engineering is also being explored as a possibility, making use of animal or human kidney scaffolds for engineering a transplantable organ...
April 20, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28429412/bone-extracellular-matrix-hydrogel-enhances-osteogenic-differentiation-of-c2c12-myoblasts-and-mouse-primary-calvarial-cells
#4
Noura Alom, Heather Peto, Glen R Kirkham, Kevin M Shakesheff, Lisa J White
Hydrogel scaffolds derived from the extracellular matrix (ECM) of mammalian tissues have been successfully used to promote tissue repair in vitro and in vivo. The objective of this study was to evaluate the osteogenic potential of ECM hydrogels prepared from demineralized and decellularized bovine bone in the presence and absence of osteogenic medium. Culture of C2C12 and mouse primary calvarial cells (mPCs) on decellularized bone ECM (bECM) and demineralized bone matrix (DBM) gels resulted in increased expression of osteogenic gene markers, including a 3...
April 21, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28429072/kidney-development-and-perspectives-for-organ-engineering
#5
REVIEW
Ganna Reint, Aleksandra Rak-Raszewska, Seppo J Vainio
Organ transplantation is currently the best strategy for treating end stage renal disease (ESRD) but the numbers of donor kidneys available are not sufficient to meet the needs of the ever-increasing ESRD population. Therefore, developments in the field of tissue engineering are necessary to provide alternative treatments. Decellularization and three-dimensional (3D) bioprinting strategies may serve as attractive novel options. Since successful tissue engineering requires an in -depth understanding of organ development and regulatory pathways, we discuss signaling in renal development and the composition of the renal extracellular matrix before presenting progress in the decellularization and 3D bioprinting fields...
April 20, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28415482/terminal-sterilization-of-equine-derived-decellularized-tendons-for-clinical-use
#6
Alessandro F Pellegata, Marta Bottagisio, Federica Boschetti, Marco Ferroni, Monica Bortolin, Lorenzo Drago, Arianna B Lovati
In the last few years, the demand for tissue substitutes has increased and decellularized matrices has been widely proposed in the medical field to restore severe damages thanks to high biocompatibility and biomechanical properties similar to the native tissues. However, biological grafts represent a potential source of contamination and disease transmission; thus, there is the need to achieve acceptable levels of sterility. Several sterilization methods have been investigated with no consensus on the outcomes in terms of minimizing structural damages and preserving functional features of the decellularized matrix for transplantation in humans...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415472/supercritical-carbon-dioxide-extracted-extracellular-matrix-material-from-adipose-tissue
#7
Jun Kit Wang, Baiwen Luo, Vipra Guneta, Liang Li, Selin Ee Min Foo, Yun Dai, Timothy Thatt Yang Tan, Nguan Soon Tan, Cleo Choong, Marcus Thien Chong Wong
Adipose tissue is a rich source of extracellular matrix (ECM) material that can be isolated by delipidating and decellularizing the tissue. However, the current delipidation and decellularization methods either involve tedious and lengthy processes or require toxic chemicals, which may result in the elimination of vital proteins and growth factors found in the ECM. Hence, an alternative delipidation and decellularization method for adipose tissue was developed using supercritical carbon dioxide (SC-CO2) that eliminates the need of any harsh chemicals and also reduces the amount of processing time required...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28412553/native-and-solubilized-decellularized-extracellular-matrix-a-critical-assessment-of-their-potential-for-improving-the-expansion-of-mesenchymal-stem-cells
#8
REVIEW
Aida Shakouri-Motlagh, Andrea J O'Connor, Shaun P Brennecke, Bill Kalionis, Daniel E Heath
Capturing the promise of mesenchymal stem cell (MSC)-based treatments is currently limited by inefficient production of cells needed for clinical therapies. During conventional ex vivo expansion, a large portion of MSCs lose the properties that make them attractive for use in cell therapies. Decellularized extracellular matrix (dECM) has recently emerged as a promising substrate for the improved expansion of MSCs. MSCs cultured on these surfaces exhibit improved proliferation capacity, maintenance of phenotype, and increased differentiation potential...
April 12, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28407495/stem-cell-delivery-in-tissue-specific-hydrogel-enabled-meniscal-repair-in-an-orthotopic-rat-model
#9
Xiaoning Yuan, Yiyong Wei, Aránzazu Villasante, Johnathan J D Ng, Derya E Arkonac, Pen-Hsiu Grace Chao, Gordana Vunjak-Novakovic
Interest in non-invasive injectable therapies has rapidly risen due to their excellent safety profile and ease of use in clinical settings. Injectable hydrogels can be derived from the extracellular matrix (ECM) of specific tissues to provide a biomimetic environment for cell delivery and enable seamless regeneration of tissue defects. We investigated the in situ delivery of human mesenchymal stem cells (hMSCs) in decellularized meniscus ECM hydrogel to a meniscal defect in a nude rat model. First, decellularized meniscus ECM hydrogel retained tissue-specific proteoglycans and collagens, and significantly upregulated expression of fibrochondrogenic markers by hMSCs versus collagen hydrogel alone in vitro...
July 2017: Biomaterials
https://www.readbyqxmd.com/read/28402966/an-injectable-nucleus-pulposus-cell-modified-decellularized-scaffold-biocompatible-material-for-prevention-of-disc-degeneration
#10
Zhi Shan, Xianfeng Lin, Shengyu Wang, Xuyang Zhang, Yichuan Pang, Shengyun Li, Tianming Yu, Shunwu Fan, Fengdong Zhao
We developed a nucleus pulposus cell (NPC)-modulated decellularized small intestinal submucosa (SIS) scaffold, and assessed the ability of this material to prevent Intervertebral disc degeneration (IVD) degeneration. Decellularized porcine SIS was squashed into particles and the biological safety and efficiency of these particles were evaluated. Next, SIS particles were seeded with rabbit NPCs, cultured for two months in vitro, decellularized again and suspended for intervertebral injection. We demonstrated that use of the decellularization protocol resulted in the removal of cellular components with maximal retention of extracellular matrix...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28402065/identification-of-a-synergistic-interaction-between-endothelial-cells-and-retinal-pigment-epithelium
#11
Carrie Spencer, Stephanie Abend, Kevin J McHugh, Magali Saint-Geniez
The retinal pigment epithelium located between the neurosensory retina and the choroidal vasculature is critical for the function and maintenance of both the photoreceptors and underlying capillary endothelium. While the trophic role of retinal pigment epithelium on choroidal endothelial cells is well recognized, the existence of a reciprocal regulatory function of endothelial cells on retinal pigment epithelium cells remained to be fully characterized. Using a physiological long-term co-culture system, we determined the effect of retinal pigment epithelium-endothelial cell heterotypic interactions on cell survival, behaviour and matrix deposition...
April 12, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28396578/comparative-characterization-of-decellularized-renal-scaffolds-for-tissue-engineering
#12
Iris Fischer, Michael Westphal, Bella Rossbach, Nicole Bethke, Krithika Hariharan, Imran Ullah, Petra Reinke, Andreas Kurtz, Harald Stachelscheid
Native extracellular matrix (ECM) provides scaffolds for tissue engineering with natural architecture and biochemical composition. Maintaining the native ECM in decellularized tissues provides cues for cells, which promote their tissue specific arrangement and function. Several approaches have been used to decellularize ECM from the kidney in order to reestablish renal tissue but their comparability is hampered because methods for decellularization and assessment of ECM vary widely. Therefore, we applied a standardized immersion protocol to decellularize porcine kidney tissue with three detergents Triton X-100, sodium dodecyl sulfate (SDS) and sodium deoxycholate (SDC) at variable temperatures...
April 11, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28395322/de-and-recellularization-of-the-pig-uterus-a-bioengineering-pilot-study%C3%A2
#13
Hannes Campo, Pedro M Baptista, Nuria López-Pérez, Amparo Faus, Irene Cervelló, Carlos Simón
Absolute uterine factor infertility, or the absence of a functional uterus, has a prevalence of 3%-5% in the general population. Despite the great strides being made in reproductive medicine, patients diagnosed with absolute uterine factor infertility remain untreatable. The only available solution has been gestational surrogacy, but recently the Brannström group presented a viable alternative by reporting the first successful live birth after uterus transplantation. Similar to other transplantations, this approach has inherent limitations such as the paucity of donor organs and the need for long-term immunosuppression...
January 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28382576/in-vivo-human-corpus-cavernosum-regeneration-fabrication-of-tissue-engineered-corpus-cavernosum-in-rat-using-the-body-as-a-natural-bioreactor
#14
Abdol-Mohammad Kajbafzadeh, Reza Abbasioun, Nastaran Sabetkish, Shabnam Sabetkish, Ali Akbar Habibi, Kamyar Tavakkolitabassi
PURPOSE: Few researches have been conducted to develop an ideal method for tissue engineering of corpus cavernosum. We produce a decellularized human corpus cavernosum scaffold and investigate the in vivo cell seeding of the scaffold after transplantation into the rat omentum. METHODS: Eight adult human male corpus cavernosum were obtained in sterile condition. After dissecting the urethra, corpus cavernosum was decellularized by inserting an 18-gauge needle into the body of the tissue...
April 5, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28380688/fabrication-and-characterization-of-a-decellularized-bovine-tendon-sheet-for-tendon-reconstruction
#15
Liang-Ju Ning, Yan-Lin Jiang, Cheng-Hao Zhang, Yi Zhang, Jie-Liang Yang, Jing Cui, Yan-Jing Zhang, Xuan Yao, Jing-Cong Luo, Ting-Wu Qin
Obtaining a performing decellularized tendon scaffold with proper dimensions and adequate availability is highly desirable. However, the combined study of complete decellularization and detailed characterization of native tendon extracellular matrix (ECM) from large animals is still lacking. In the present study, we developed a new decellularization protocol, including physical methods and enzymatic solutions for processing bovine Achilles tendons, and produced a decellularized bovine tendon sheet (DBTS) scaffold for tendon reconstruction...
April 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28372287/organ-reconstruction-dream-or-reality-for%C3%A2-the%C3%A2-future
#16
J-F Stoltz, L Zhang, J S Ye, N De Isla
The relevance of research on reconstructed organs is justified by the lack of organs available for transplant and the growing needs for the ageing population. The development of a reconstructed organ involves two parallel complementary steps: de-cellularization of the organ with the need to maintain the structural integrity of the extracellular matrix and vascular network and re-cellularization of the scaffold with stem cells or resident cells.Whole organ engineering for liver, heart, lung or kidneys, is particularly difficult because of the structural complexity of organs and heterogeneity of cells...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28372286/research-progress-in-liver-tissue-engineering
#17
Lei Zhang, Zheng Guan, Jun-Song Ye, Yan-Feng Yin, Jean-François Stoltz, Natalia de Isla
Liver transplantation is the definitive treatment for patients with end-stage liver diseases (ESLD). However, it is hampered by shortage of liver donor. Liver tissue engineering, aiming at fabricating new livers in vitro, provides a potential resolution for donor shortage. Three elements need to be considered in liver tissue engineering: seeding cell resources, scaffolds and bioreactors. Studies have shown potential cell sources as hepatocytes, hepatic cell line, mesenchymal stem cells and others. They need scaffolds with perfect biocompatiblity, suitable micro-structure and appropriate degradation rate, which are essential charateristics for cell attachment, proliferation and secretion in forming extracellular matrix...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28371271/development-of-hybrid-scaffolds-with-natural-extracellular-matrix-deposited-within-synthetic-polymeric-fibers
#18
Ritu Goyal, Maria E Vega, Alexandra K Pastino, Shivani Singh, Murat Guvendiren, Joachim Kohn, N Sanjeeva Murthy, Jean E Schwarzbauer
A major challenge of tissue engineering is to generate materials that combine bioactivity with stability in a form that captures the robust nature of native tissues. Here we describe a procedure to fabricate a novel hybrid extracellular matrix (ECM) - synthetic scaffold biomaterial by cell-mediated deposition of ECM within an electrospun fiber mat. Synthetic polymer fiber mats were fabricated using poly(desamino tyrosyl-tyrosine carbonate) (PDTEC) co-spun with poly(ethylene glycol) (PEG) used as a sacrificial polymer...
March 28, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28371268/aging-of-the-skeletal-muscle-extracellular-matrix-drives-a-stem-cell-fibrogenic-conversion
#19
Kristen M Stearns-Reider, Antonio D'Amore, Kevin Beezhold, Benjamin Rothrauff, Loredana Cavalli, William R Wagner, David A Vorp, Alkiviadis Tsamis, Sunita Shinde, Changqing Zhang, Aaron Barchowsky, Thomas A Rando, Rocky S Tuan, Fabrisia Ambrosio
Age-related declines in skeletal muscle regeneration have been attributed to muscle stem cell (MuSC) dysfunction. Aged MuSCs display a fibrogenic conversion, leading to fibrosis and impaired recovery after injury. Although studies have demonstrated the influence of in vitro substrate characteristics on stem cell fate, whether and how aging of the extracellular matrix (ECM) affects stem cell behavior has not been investigated. Here, we investigated the direct effect of the aged muscle ECM on MuSC lineage specification...
March 30, 2017: Aging Cell
https://www.readbyqxmd.com/read/28364144/current-advances-in-the-development-of-natural-meniscus-scaffolds-innovative-approaches-to-decellularization-and-recellularization
#20
REVIEW
Yunbin Chen, Jiaxin Chen, Zeng Zhang, Kangliang Lou, Qi Zhang, Shengyu Wang, Jinhu Ni, Wenyue Liu, Shunwu Fan, Xianfeng Lin
The increasing rate of injuries to the meniscus indicates the urgent need to develop effective repair strategies. Irreparably damaged menisci can be replaced and meniscus allografts represent the treatment of choice; however, they have several limitations, including availability and compatibility. Another approach is the use of artificial implants but their chondroprotective activities are still not proved clinically. In this situation, tissue engineering offers alternative natural decellularized extracellular matrix (ECM) scaffolds, which have shown biomechanical properties comparable to those of native menisci and are characterized by low immunogenicity and promising regenerative potential...
March 31, 2017: Cell and Tissue Research
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