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Decellularized matrix

Weimin Guo, Xifu Zheng, Weiguo Zhang, Mingxue Chen, Zhenyong Wang, Chunxiang Hao, Jingxiang Huang, Zhiguo Yuan, Yu Zhang, Mingjie Wang, Jiang Peng, Aiyuan Wang, Yu Wang, Xiang Sui, Wenjing Xu, Shuyun Liu, Shibi Lu, Quanyi Guo
Articular cartilage lacks a blood supply and nerves. Hence, articular cartilage regeneration remains a major challenge in orthopedics. Decellularized extracellular matrix- (ECM-) based strategies have recently received particular attention. The structure of native cartilage exhibits complex zonal heterogeneity. Specifically, the development of a tissue-engineered scaffold mimicking the aligned structure of native cartilage would be of great utility in terms of cartilage regeneration. Previously, we fabricated oriented PLGA/ACECM (natural, nanofibrous, articular cartilage ECM) composite scaffolds...
2018: Stem Cells International
Saeed Zahiri, Elahe Masaeli, Elahe Poorazizi, Mohammad Hossein Nasr-Esfahani
Current studies based on regenerative medicine suggest, decellularized extracellular matrix (DC-ECM) components can regulate cell phenotype. In this regard, it is believed, presence of cartilage extracellular matrix particles in culture condition could produce physical and biochemical supportive cues for chondrogenesis. In this study, DC-ECM nanoparticles with average size of 61.5± 22.4 nm were produced by decellularization and mechanical processing. Homogenous distribution and nano scale size of yield particles were observed by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) microscopy imaging...
April 17, 2018: Journal of Biomedical Materials Research. Part A
Jia Dong, Mei Yu, Yan Zhang, Yin Yin, Weidong Tian
Natural and synthetic materials have been developed to provide a three-dimensional (3-D) scaffold that mimics the extracellular matrix properties for tissue regeneration and reconstruction. On account of the excellent biological and mechanical properties, diverse decellularized tissue materials have been developed as natural scaffolds. Among them, decellularized adipose tissue (DAT) has generated great interest in recent years due to the extensiveness of the source and the eximious potential to enhance the regeneration and repair of damaged tissues...
April 17, 2018: Journal of Biomedical Materials Research. Part A
Jimmy Su, Simon C Satchell, Ramille N Shah, Jason A Wertheim
Hydrogels, highly-hydrated crosslinked polymer networks, closely mimic the microenvironment of native extracellular matrix (ECM) and thus present as ideal platforms for three-dimensional cell culture. Hydrogels derived from tissue- and organ-specific decellularized ECM (dECM) may retain bioactive signaling cues from the native tissue or organ that could in turn modulate cell-material interactions and response. In this study, we demonstrate that porcine kidney dECM can be processed to form hydrogels suitable for cell culture and encapsulation studies...
April 17, 2018: Journal of Biomedical Materials Research. Part A
Ksenia I Lukanina, Timofei E Grigoriev, Sergey V Krasheninnikov, Vissarion G Mamagulashvilli, Roman A Kamyshinsky, Sergey N Chvalun
A novel high-tech composite biomimetic matrixes for a wide range of medical purposes were prepared. The structure of scaffolds was inspired by the architecture of native decellularized tissue: material consists of a sponge and fibrous components of different spatial geometry based on cellulose acetate with collagen or chitosan filler. The fibrous component was prepared by electrospinning, the sponge - freeze-drying technique. The influence of main technological parameters, such as freeze mode, polymer type and concentration, etc...
July 1, 2018: Carbohydrate Polymers
Tao Lin, Sheng Liu, Shihao Chen, Shuai Qiu, Zilong Rao, Jianghui Liu, Shuang Zhu, Liwei Yan, Haiquan Mao, Qingtang Zhu, Daping Quan, Xiaolin Liu
Decellularized matrix hydrogels derived from tissues or organs have been used for tissue repair due to their biocompatibility, tunability, and tissue-specific extracellular matrix (ECM) components. However, the preparation of decellularized peripheral nerve matrix hydrogels and their use to repair nerve defects have not been reported. Here, we developed a hydrogel from porcine decellularized nerve matrix (pDNM-G), which was confirmed to have minimal DNA content and retain collagen and glycosaminoglycans content, thereby allowing gelatinization...
April 9, 2018: Acta Biomaterialia
Yang Zhu, Sato Hideyoshi, Hongbin Jiang, Yasumoto Matsumura, Jenna L Dziki, Samuel T LoPresti, Luai Huleihel, Gabriela N F Faria, Leah C Fuhrman, Ricardo Lodono, Stephen F Badylak, William R Wagner
Biodegradable injectable hydrogels have been extensively studied and evaluated in various medical applications such as for bulking agents, drug delivery reservoirs, temporary barriers, adhesives, and cell delivery matrices. Where injectable hydrogels are intended to facilitate a healing response, it may be desirable to encourage rapid cellular infiltration into the hydrogel volume from the tissue surrounding the injection site. In this study, we developed a platform technique to rapidly form pores in a thermally responsive injectable hydrogel, poly(NIPAAm-co-VP-co-MAPLA) by using mannitol particles as porogens...
April 9, 2018: Acta Biomaterialia
Maria De Luca
The extracellular matrix (ECM) is an intricate network that provides structural and anchoring support to cells in order to stabilize cell morphology and tissue architecture. The ECM also controls many aspects of the cell's dynamic behavior and fate through its ongoing, bidirectional interaction with cells. These interactions between the cell and components of the surrounding ECM are implicated in several biological processes, including development and adult tissue repair in response to injury, throughout the lifespan of multiple species...
April 4, 2018: Mechanisms of Ageing and Development
Kamyar Esmaeili Pourfarhangi, Shohreh Mashayekhan, Sasan Ghanbari Asl, Zahra Hajebrahimi
High rates of mortality and morbidity stemming from cardiovascular diseases unveil extreme limitations in current therapies despite enormous advances in medical and pharmaceutical sciences. Following myocardial infarction (MI), parts of myocardium undergo irreversible remodeling and is substituted by a scar tissue which eventually leads to heart failure (HF). To address this issue, cardiac patches have been utilized to initiate myocardial regeneration. In this study, a porous cardiac patch is fabricated using a mixture of decellularized myocardium extracellular matrix (ECM) and chitosan (CS)...
April 3, 2018: Biologicals: Journal of the International Association of Biological Standardization
Yaling Yu, Li Liu, Jianse Zhang, Zairong Wei, Jin Mei
The decellularized (DC) scaffolds retain three-dimensional structures for the stimulation of cell growth, with components of the extracellular matrix (ECM) relatively conserved between species. The strategy based on decellularized scaffolds provides a new way for organ regeneration, with a number of prominent advances having been reported in the past few years. While their lack of biomechanical strength and excessive degradation limit the clinical applications, therefore it is urgent to modify the DC scaffolds to improve the performance...
April 6, 2018: Methods in Molecular Biology
Remy V Rabinovich, Steven J Lee
Wrist arthritis is a common problem. When managed early, motion-sparing procedures can be performed with successful outcomes. Proximal row carpectomy (PRC) has demonstrated good functional results and high patient satisfaction with up to 20-year follow-up. The majority of patients with longer follow-up have, however, exhibited radiographic signs of progressive degeneration of the radiocapitate space. Although radiographic changes have not been shown to correlate with wrist pain and function, it remains a concern and questions the durability of the radiocapitate articulation with continued patient follow-up, especially those who underwent the original procedure at a younger age...
April 2018: Journal of Hand Surgery
Oskar Rosmark, Emma Åhrman, Catharina Müller, Linda Elowsson Rendin, Leif Eriksson, Anders Malmström, Oskar Hallgren, Anna-Karin Larsson-Callerfelt, Gunilla Westergren-Thorsson, Johan Malmström
Remodelling of the extracellular matrix is accomplished by altering the balance between matrix macromolecule production and degradation. However, it is not well understood how cells balance production of new matrix molecules and degradation of existing ones during tissue remodelling and regeneration. In this study, we used decellularized lung scaffolds repopulated with allogenic lung fibroblasts cultured with stable isotope labelled amino acids to quantify the balance between matrix production and degradation at a proteome-wide scale...
April 3, 2018: Scientific Reports
Xinyu Qiu, Shiyu Liu, Hao Zhang, Bin Zhu, Yuting Su, Chenxi Zheng, Rong Tian, Miao Wang, Huijuan Kuang, Xinyi Zhao, Yan Jin
BACKGROUND: Skeletal muscle plays an important role in the body's physiology but there are still no effective treatments for volumetric muscle loss (VML) resulting from severe traumatic injury or tumor excision. Recent studies show that a tissue engineering strategy using a compound containing mesenchymal stem cells (MSCs) and decellularized extracellular matrix (ECM) scaffold generates significant regenerative effects on VML injury, but the underlying mechanisms are not fully understood...
April 3, 2018: Stem Cell Research & Therapy
Margarita Zhuravleva, Zarema Gilazieva, Timofei E Grigoriev, Alexey D Shepelev, Timur Kh Tenchurin, Roman Kamyshinsky, Sergey V Krasheninnikov, Sergei Orlov, Gina Caralogli, Svetlana Archipova, Mark J Holterman, Mikhail Mavlikeev, Roman V Deev, Sergei N Chvalun, Paolo Macchiarini
Artificial tissue-engineered grafts offer a potential alternative to autologous tissue grafts for patients, which can be traumatic. After decellularizing Papio hamadryas esophagus and studying the morphology and physical properties of the extracellular matrix (ECM), we generated electrospun polyamide-6 based scaffolds to mimic it. The scaffolds supported a greater mechanical load than the native ECM and demonstrated similar 3D microstructure, with randomly aligned fibers, 90% porosity, 29 μm maximal pore size, and average fiber diameter of 2...
March 30, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Matthias Becker, Janita A Maring, Maria Schneider, Aarón X Herrera Martin, Martina Seifert, Oliver Klein, Thorsten Braun, Volkmar Falk, Christof Stamm
There is a growing need for scaffold material with tissue-specific bioactivity for use in regenerative medicine, tissue engineering, and for surgical repair of structural defects. We developed a novel composite biomaterial by processing human cardiac extracellular matrix (ECM) into a hydrogel and combining it with cell-free amniotic membrane via a dry-coating procedure. Cardiac biocompatibility and immunogenicity were tested in vitro using human cardiac fibroblasts, epicardial progenitor cells, murine HL-1 cells, and human immune cells derived from buffy coat...
March 29, 2018: International Journal of Molecular Sciences
Kwang Il Lee, Jung Soo Lee, Kyoung Tak Kang, Young Bock Shim, Young Sik Kim, Ju Woong Jang, Seong Hwan Moon, Darryl D D'Lima
BACKGROUND: Anterior cruciate ligament (ACL) reconstruction is the current standard of care for ACL tears. However, the results are not consistently successful; autografts or allografts have certain disadvantages; and synthetic grafts have had poor clinical results. PURPOSE: To determine if recellularization of decellularized tendons combined with mechanical stimulation in a bioreactor could replicate the mechanical properties of the native ACL and be successfully used for ACL reconstruction in vivo...
March 1, 2018: American Journal of Sports Medicine
Jing Chu, Panpan Shi, Xiaoyuan Deng, Ying Jin, Hao Liu, Maosheng Chen, Xue Han, Hanping Liu
Significantly effective therapies need to be developed for chronic non-healing diabetic wounds. In this work, the topical transplantation of mesenchymal stem cell (MSC) seeded on an acellular dermal matrix (ADM) scaffold is proposed as a novel therapeutic strategy for diabetic cutaneous wound healing. GFP-labeled MSCs were co-cultured with an ADM scaffold that was decellularized from normal mouse skin. These cultures were subsequently transplanted as a whole into the full-thickness cutaneous wound site in streptozotocin (STZ)-induced diabetic mice...
March 25, 2018: Journal of Biophotonics
Martin Boháč, Ľuboš Danišovič, Ján Koller, Jana Dragúňová, Ivan Varga
Acellular matrices are used for various purposes and they have been studied extensively for their potential roles in regenerating tissues or organs. The acellular matrix generates physiological cues that mimic the native tissue microenvironment. Acellular dermal matrix (ADM) is a soft connective tissue graft generated by a decellularization process that preserves the intact extracellular skin matrix. Upon implantation, this structure serves as a scaffold for donor-side cells to facilitate subsequent incorporation and revascularization...
January 22, 2018: European Journal of Histochemistry: EJH
Kamal Hany Hussein, Tarek Saleh, Ebtehal Ahmed, Ho-Hyun Kwak, Kyung-Mee Park, Se-Ran Yang, Byung-Jae Kang, Ki-Young Choi, Kyung-Sun Kang, Heung-Myong Woo
Whole kidney decellularization is a promising approach in regenerative medicine for engineering a functional organ. The reaction of the potential host depends on the biocompatibility of these decellularized constructs. Despite the proven ability of decellularized kidney scaffolds to guide cell attachment and growth, little is known about biocompatibility and hemocompatibility of these scaffolds. Our aim is to prepare decellularized kidneys of a clinically relevant size and evaluate its biocompatibility and hemocompatibility...
March 22, 2018: Journal of Biomedical Materials Research. Part A
Radwa Ayman Salah, Ihab K Mohamed, Nagwa El-Badri
Developing effective stem cell-based therapies requires the design of complex in vitro culture systems for accurate representation of the physiological stem cell niche. Human amniotic membrane (hAM) has been successfully used in clinical grafting applications due to its unique biological and regenerative properties. Decellularized hAM (d-hAM) has been previously applied to the culture of human bone marrow mesenchymal stem cells (hMSCs), promoting their expansion and differentiation into adipogenic and osteogenic lineages...
March 22, 2018: Journal of Molecular Histology
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