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https://www.readbyqxmd.com/read/29057501/different-approaches-to-heart-valve-decellularization-a-comprehensive-overview-of-the-past-30%C3%A2-years
#1
REVIEW
Filippo Naso, Alessandro Gandaglia
Xenogeneic decellularized heart valve scaffolds have the potential to overcome the limitations of existing bioprosthetic heart valves that have limited duration due to calcification and tissue degeneration phenomena. This article presents a review of 30 years of decellularization approaches adopted in cardiovascular tissue engineering, with a focus on the use, either individually or in combination, of different detergents. The safety and efficacy of cell-removal procedures are specifically reported and discussed, as well as the structure and biomechanics of the treated extracellular matrix (ECM)...
October 22, 2017: Xenotransplantation
https://www.readbyqxmd.com/read/29055775/bio-artificial-tongue-with-tongue-extracellular-matrix-and-primary-taste-cells
#2
Jung Seung Lee, Ann-Na Cho, Yoonhee Jin, Jin Kim, Suran Kim, Seung-Woo Cho
Artificial taste devices for tastant sensing and taste information standardization are attracting increasing attention with the exponential growth of the food and beverage industries. Despite recent developments in artificial taste sensors incorporating polymers, lipid membranes, and synthetic vesicles, current devices have limited functionality and sensitivity, and are complex to manufacture. Moreover, such synthetic systems cannot simulate the taste signal transmissions that are critical for complicated taste perception...
October 10, 2017: Biomaterials
https://www.readbyqxmd.com/read/29052367/decellularized-caprine-liver-extracellular-matrix-as-a-2d-substrate-coating-and-3d-hydrogel-platform-for-vascularized-liver-tissue-engineering
#3
Tarun Agarwal, Rajan Narayan, Somnath Maji, Sudip Kumar Ghosh, Tapas Kumar Maiti
Development of a vascularized liver tissue construct is a need of an hour to circumvent the current demand of liver transplantation in healthcare sector. An appropriate matrix must support liver cell viability, functionality, and development of microvasculature. With this perspective, here, we report the use of decellularized Caprine liver ECM (CLECM) derived hydrogel for tissue engineering applications. First, CLECM was used as a substrate coating material for 2D hepatocyte culture. HepG2 cells cultured on CLECM coated surface showed higher albumin, urea, glycogen and GAGs synthesis in comparison to collagen coated surface (taken as control for the study)...
October 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29050915/decellularized-matrix-of-adipose-derived-mesenchymal-stromal-cells-enhanced-retinal-progenitor-cell-proliferation-via-the-akt-erk-pathway-and-neuronal-differentiation
#4
Jing Ji, Dandan Zhang, Wei Wei, Bingqiao Shen, Yi Zhang, Yuyao Wang, Zhimin Tang, Ni Ni, Hao Sun, Jiaqiang Liu, Xianqun Fan, Ping Gu
BACKGROUND AIMS: Retinal progenitor cells (RPCs) are a promising cell therapy treatment for retinal degenerative diseases. However, problems with limited proliferation ability and differentiation preference toward glia rather than neurons restrict the clinical application of these RPCs. The extracellular matrix (ECM) has been recognized to provide an appropriate microenvironment to support stem cell adhesion and direct cell behaviors, such as self-renewal and differentiation. METHODS: In this study, decellularized matrix of adipose-derived mesenchymal stromal cells (DMA) was manufactured using a chemical agent method (0...
October 16, 2017: Cytotherapy
https://www.readbyqxmd.com/read/29049912/globular-chitosan-treatment-of-bovine-jugular-veins-evidence-of-anticalcification-efficacy-in-the-subcutaneous-rat-model
#5
Anna A Krasilnikova, David S Sergeevichev, Vladislav V Fomenko, Alexander A Korobeynikov, Maria B Vasilyeva, Alexander S Yunoshev, Alexander M Karaskov, Evgeny A Pokushalov
Vascular xenografts are widely used in cardiovascular surgery as an alternative to autologous vessels and vascular allografts. Calcification is one of the main drawbacks of vascular grafts, especially among young patients and children. Among different anticalcification approaches, chitosan emerges as a highly promising candidate due to its versatility, natural origin, and biocompatibility. We investigated the anticalcification efficacy of globular chitosan ("Chitozol") as it demonstrated the improved rate of water solubility as compared with conventional linear macromolecules of chitosan...
September 14, 2017: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/29048241/nanofibrous-electrospun-hdecm-based-hybrid-scaffold-as-wound-dressing-for-reducing-scarring
#6
Tae Hee Kim, Youngmee Jung, Soo Hyun Kim
Produced via electrospinning, poly(l-lactide-co-caprolactone) (PLCL) membranes, which have a porous structure and are biodegradable, are of interest in various medical fields. The porous-structured electrospun membrane is particularly interesting because of several favorable properties: it exudes fluid from the wound, does not build up under the wound covering, and does not cause wound desiccation. Moreover, extracellular matrix (ECM)-based structures derived by tissue decellularization have application as engineered tissue scaffolds and as supports for cellular regeneration...
October 19, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29037855/nephrosphere-derived-cells-are-induced-to-multilineage-differentiation-when-cultured-on-human-decellularized-kidney-scaffolds
#7
Silvia Bombelli, Chiara Meregalli, Carla Scalia, Giorgio Bovo, Barbara Torsello, Sofia De Marco, Massimiliano Cadamuro, Paolo Viganò, Guido Strada, Giorgio Cattoretti, Cristina Bianchi, Roberto A Perego
In end-stage chronic kidney disease, the option of organ transplantation is limited due to the scarce availability of kidneys. The combination of stem cell research, regenerative medicine, and tissue engineering seems a promising approach to produce new transplantable kidneys. Currently, the possibility to repopulate naturally obtained scaffolds with cells of different sources is advancing. Our aim was to test, for the first time, whether the nephrosphere (NS) cells, composed by renal stem/progenitor-like cells, were able to repopulate different nephron portions of renal extracellular matrix scaffolds obtained after decellularization of human renal tissue slices...
October 13, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/29037258/transplantation-of-isl1-cardiac-progenitor-cells-in-small-intestinal-submucosa-improves-infarcted-heart-function
#8
Lingjun Wang, Elizabeth M Meier, Shuo Tian, Ienglam Lei, Liu Liu, Shaoxiang Xian, Mai T Lam, Zhong Wang
BACKGROUND: Application of cardiac stem cells combined with biomaterial scaffold is a promising therapeutic strategy for heart repair after myocardial infarction. However, the optimal cell types and biomaterials remain elusive. METHODS: In this study, we seeded Isl1(+) embryonic cardiac progenitor cells (CPCs) into decellularized porcine small intestinal submucosa extracellular matrix (SIS-ECM) to assess the therapeutic potential of Isl1(+) CPCs and the biocompatibility of SIS-ECM with these cells...
October 16, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29035736/mechanotransduction-of-human-pluripotent-stem-cells-cultivated-on-tunable-cell-derived-extracellular-matrix
#9
In Gul Kim, Chang-Hyun Gil, Joseph Seo, Soon-Jung Park, Ramesh Subbiah, Taek-Hee Jung, Jong Soo Kim, Young-Hoon Jeong, Hyung-Min Chung, Jong Ho Lee, Man Ryul Lee, Sung-Hwan Moon, Kwideok Park
Cell-derived matrices (CDM) are becoming an attractive alternative to conventional biological scaffolding platforms due to its unique ability to closely recapitulate a native extracellular matrix (ECM) de novo. Although cell-substrate interactions are recognized to be principal in regulating stem cell behavior, very few studies have documented the acclimation of human pluripotent stem cells (hPSCs) on pristine and altered cell-derived matrices. Here, we investigate crosslink-induced mechanotransduction of hPSCs cultivated on decellularized fibroblast-derived matrices (FDM) to explore cell adhesion, growth, migration, and pluripotency in various biological landscapes...
October 9, 2017: Biomaterials
https://www.readbyqxmd.com/read/29035553/construction-of-antithrombotic-tissue-engineered-blood-vessel-via-reduced-graphene-oxide-based-dual-enzyme-biomimetic-cascade
#10
Da Huo, Ge Liu, Yanzhao Li, Yuxin Wang, Ge Guan, Mingcan Yang, Keyu Wei, Jingyuan Yang, Lingqin Zeng, Gang Li, Wen Zeng, Chuhong Zhu
Thrombosis is one of the biggest obstacles in the clinical application of small-diameter tissue-engineered blood vessels (TEBVs). The implantation of an unmodified TEBV will lead to platelet aggregation and further activation of the coagulation cascade, in which the high concentration of adenosine diphosphate (ADP) that is released by platelets plays an important role. Inspired by the phenomenon that endothelial cells continuously generate endogenous antiplatelet substances via enzymatic reactions, we designed a reduced graphene oxide (RGO) based dual-enzyme biomimetic cascade to successively convert ADP into adenosine monophosphate (AMP) and AMP into adenosine...
October 20, 2017: ACS Nano
https://www.readbyqxmd.com/read/29024376/evaluation-of-collagen-foam-poly-l-lactic-acid-nanofiber-mesh-and-decellularized-matrices-for-corneal-regeneration
#11
Bahar Aslan, Selcan Guler, Atakan Tevlek, Halil Murat Aydin
Corneal tissue engineering efforts to obtain corneal tissue matrices through various types of materials for the replacement of damaged tissues. In this study, three different corneal constructs were prepared and evaluated in terms of morphological, optical, and biological characteristics. Type-I collagen was used to obtain collagen foam scaffolds through dehydrothermal crosslinking, while poly(l-lactic acid) (PLLA) was used to produce both random and aligned oriented electrospun corneal constructs. Bovine corneas were decellularized as third matrix...
October 12, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29019588/ultrastructural-analysis-and-residual-dna-evaluation-of-rabbit-vein-scaffold
#12
Matheus Bertanha, Marcone Lima Sobreira, Ana Lívia de Carvalho Bovolato, Jaqueline de Carvalho Rinaldi, Patricia Pintor Reis, Andrei Moroz, Leonardo Nazario de Moraes, Elenice Deffune
PURPOSE: To investigate the ultrastructural characteristics and analysis of residual DNA in scaffold models, produced with decellularized vena cava in an experimental model with rabbits. METHODS: Three groups were created for ultrastructural and residual DNA analysis: group 1 - control, consisting of samples of vena cava in natura; group 2 - SD, consisting of vein fragments submitted to 2% sodium deoxycholate decellularization by shaking (160rpm - Shaker News Brunswick Scientific®) for 1 hour at controlled temperature shaker at 37°C; group 3 - SDS, consisting of vein fragments submitted to 1% sodium dodecyl sulfate decellularization under the same previous condition, for 2 hours...
September 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29018244/bioengineering-of-functional-human-induced-pluripotent-stem-cell-derived-intestinal-grafts
#13
Kentaro Kitano, Dana M Schwartz, Haiyang Zhou, Sarah E Gilpin, Gregory R Wojtkiewicz, Xi Ren, Cesar A Sommer, Amalia V Capilla, Douglas J Mathisen, Allan M Goldstein, Gustavo Mostoslavsky, Harald C Ott
Patients with short bowel syndrome lack sufficient functional intestine to sustain themselves with enteral intake alone. Transplantable vascularized bioengineered intestine could restore nutrient absorption. Here we report the engineering of humanized intestinal grafts by repopulating decellularized rat intestinal matrix with human induced pluripotent stem cell-derived intestinal epithelium and human endothelium. After 28 days of in vitro culture, hiPSC-derived progenitor cells differentiate into a monolayer of polarized intestinal epithelium...
October 10, 2017: Nature Communications
https://www.readbyqxmd.com/read/28984044/an-engineered-tendon-ligament-bioscaffold-derived-from-decellularized-and-demineralized-cortical-bone-matrix
#14
Jie-Liang Yang, Xuan Yao, Quan Qing, Yi Zhang, Yan-Lin Jiang, Liang-Ju Ning, Jing-Cong Luo, Ting-Wu Qin
Demineralized bone matrix (DBM), as an extracellular matrix (ECM), has had limited use as a medical replacement although studies have reported a possibility for its use in tendon or ligament tissue engineering. To be an acid-extracted organic matrix, DBM contains much of bone protein, with a small amount of inorganic solids and some cell debris. However, cell debris is a critical factor that triggers inflammatory reaction in clinical reconstructions using ECM. In this study, we used a protocol incorporating the use of detergent with nuclease treatment to prepare decellularized DBM (DCDBM)...
October 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28976282/injectable-co-gels-of-collagen-and-decellularized-vascular-matrix-improve-msc-based-therapy-for-acute-kidney-injury
#15
Shuang Huang, Yajian Li, Xinning Wang, Xin Ma, Xu Zhang
Transplantation of mesenchymal stem cells (MSCs) is promising for treatment of acute kidney injury (AKI), but their therapeutic effects are often limited under normal conditions. In this study, we prepared the co-gels of decellularized vascular matrix and collagen, and investigated whether the co-gels increase the therapeutic potentials of MSCs on AKI. In vitro studies indicated that the co-gels enhanced the paracrine effects of MSCs, and significantly reduced the apoptosis of MSCs under oxidative environments...
October 13, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28972946/recent-advances-in-decellularization-and-recellularization-for-tissue-engineered-liver-grafts
#16
Yujia Wang, Clara T Nicolas, Harvey S Chen, Jeffery J Ross, Silvana B De Lorenzo, Scott L Nyberg
Liver transplantation from deceased or living human donors remains the only proven option for patients with end-stage liver disease. However, the shortage of donor organs is a significant clinical concern that has led to the pursuit of tissue-engineered liver grafts generated from decellularized liver extracellular matrix and functional cells. Investigative efforts on optimizing both liver decellularization and recellularization protocols have been made in recent decades. In the current review, we briefly summarize these advances, including the generation of high-quality liver extracellular matrix scaffolds, evaluation criteria for quality control, modification of matrix for enhanced properties, and reseeding strategies...
2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/28971683/hydroxylamine-chemical-digestion-for-insoluble-extracellular-matrix-characterization
#17
Alexander S Barrett, Matthew J Wither, Ryan C Hill, Monika Dzieciatkowska, Angelo D'Alessandro, Julie A Reisz, Kirk C Hansen
The extracellular matrix (ECM) is readily enriched by decellularizing tissues with non-denaturing detergents to solubilize and deplete the vast majority of cellular components. This approach has been used extensively to generate ECM scaffolds for regenerative medicine technologies and in 3D cell culture to model how the ECM contributes to disease progression. A highly enriched ECM fraction can then be generated using a strong chaotrope buffer that is compatible with downstream bottom-up proteomic analysis or 3D cell culture experiments after extensive dialysis...
October 3, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28967085/tracheal-decellularization-using-a-combination-of-chemical-physical-and-bioreactor-methods
#18
Yourka D Tchoukalova, Justin M Hintze, Richard E Hayden, David G Lott
INTRODUCTION: The purpose of this study was to compare different decellularization protocols with the conventional detergent enzymatic method (DEM) using continuous agitation. METHODS: The first experiment compared conventional DEM with sonication and lyophilization+freeze-thaw cycles. A second experiment was carried out to compare time-adjusted DEM (2-hour instead of 4-hour incubations with 4% deoxycholate) to decellularization in a bioreactor. Cellularity was determined by DNA-quantitation, H&E-staining and immunostaining for major histocompatibility complex-1 (MHC-1)...
September 28, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28963712/3d-decellularized-native-extracellular-matrix-scaffold-for-in-vitro-culture-expansion-of-human-wharton-s-jelly-derived-mesenchymal-stem-cells-hwj-mscs
#19
Balasubramanian Sundaram, Anne George Cherian, Sanjay Kumar
Mesenchymal stem cells (MSCs) are derived from Wharton's jelly tissue of the human umbilical cord. Given appropriate culture conditions, these cells can self-renew and differentiate into multiple cell types across the lineages. Among the properties exhibited by these cells, immunomodulation through secretion of trophic factors has been widely exploited in a broad spectrum of preclinical/clinical regenerative applications. Moreover, the extracellular matrix is found to play a major role apart from niche cells in determining stem cell fate including that of MSCs...
September 26, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28962583/engineering-human-ventricular-heart-muscles-based-on-a-highly-efficient-system-for-purification-of-human-pluripotent-stem-cell-derived-ventricular-cardiomyocytes
#20
Bin Li, Hui Yang, Xiaochen Wang, Yongkun Zhan, Wei Sheng, Huanhuan Cai, Haoyang Xin, Qianqian Liang, Ping Zhou, Chao Lu, Ruizhe Qian, Sifeng Chen, Pengyuan Yang, Jianyi Zhang, Weinian Shou, Guoying Huang, Ping Liang, Ning Sun
BACKGROUND: Most infarctions occur in the left anterior descending coronary artery and cause myocardium damage of the left ventricle. Although current pluripotent stem cells (PSCs) and directed cardiac differentiation techniques are able to generate fetal-like human cardiomyocytes, isolation of pure ventricular cardiomyocytes has been challenging. For repairing ventricular damage, we aimed to establish a highly efficient purification system to obtain homogeneous ventricular cardiomyocytes and prepare engineered human ventricular heart muscles in a dish...
September 29, 2017: Stem Cell Research & Therapy
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