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https://www.readbyqxmd.com/read/28524086/dietary-green-pea-protects-against-dss-induced-colitis-in-mice-challenged-with-high-fat-diet
#1
Shima Bibi, Luís Fernando de Sousa Moraes, Noelle Lebow, Mei-Jun Zhu
Obesity is a risk factor for developing inflammatory bowel disease. Pea is unique with its high content of dietary fiber, polyphenolics, and glycoproteins, all of which are known to be health beneficial. We aimed to investigate the impact of green pea (GP) supplementation on the susceptibility of high-fat diet (HFD)-fed mice to dextran sulfate sodium (DSS)-induced colitis. Six-week-old C57BL/6J female mice were fed a 45% HFD or HFD supplemented with 10% GP. After 7-week dietary supplementation, colitis was induced by adding 2...
May 18, 2017: Nutrients
https://www.readbyqxmd.com/read/28521868/metabolic-syndrome-enhances-endoplasmic-reticulum-oxidative-stress-and-leukocyte-endothelium-interactions-in-pcos
#2
Celia Bañuls, Susana Rovira-Llopis, Aranzazu Martinez de Marañon, Silvia Veses, Ana Jover, Marcelino Gomez, Milagros Rocha, Antonio Hernandez-Mijares, Victor M Victor
OBJECTIVE: Polycystic ovary syndrome (PCOS) is associated with insulin resistance, which can lead to metabolic syndrome (MetS). Oxidative stress and leukocyte-endothelium interactions are related to PCOS. Our aim was to evaluate whether the presence of MetS in PCOS patients can influence endoplasmic reticulum (ER) and oxidative stress and leukocyte-endothelium interactions. MATERIAL AND METHODS: This was a prospective controlled study conducted in an academic medical center...
June 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28510249/mechanism-of-pingyangmycin-induced-apoptosis-of-cultured-human-umbilical-vein-endothelial-cells
#3
Y D Huang, X Tong, Q Chen, S W Xia, Y Zhuo, Z H Kang, X Y Xue
In this study, we investigated the effects of pingyangmycin (PYM) on the growth inhibition and apoptosis of human umbilical vein endothelial cells (HUVEC). In this study, we aimed to explore the optimal concentration of PYM to induce the apoptosis of HUVEC and to determine its mechanism of action. After treatment of HUVEC with different concentrations of PYM for 24 h, cell counting kit-8 (CCK-8) was used to detect growth inhibiting effects. Annexin V-FITC/propidium iodide stain was used to detect apoptosis, and western blot was used to detect the expression of glucose-related protein 78 (GPR78) and C/EBP homologous protein (CHOP) endoplasmic reticulum stress proteins...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28506771/15d-pgj2-as-an-endoplasmic-reticulum-stress-manipulator-in-multiple-myeloma-in-vitro-and-in-vivo
#4
Marcelo Sperandio, Ana Paula D Demasi, Elizabeth F Martinez, Sara O Saad, Fernando V Pericole, Karla P Vieira, Nadir S Freitas, Vera C Araújo, Amy Louise Brown, Juliana Trindade Clemente-Napimoga, Marcelo Henrique Napimoga
Multiple myeloma (MM) is characterised by intense protein folding and, consequently endoplasmic reticulum (ER) stress. The prostaglandin 15d-PGJ2 is able to raise oxidative stress levels within the cell and potentially trigger cell death. The aim of this study was to evaluate the antineoplastic effect of 15d-PGJ2 on MM in vitro and in vivo via ER and oxidative stress pathways. MM.1R and MM.1S cell lines were treated with 15d-PGJ2 at 1-10μM and evaluated with regard to proliferation, mRNA expression of PRDX1, PRDX4, GRP78, GRP94, CHOP, BCL-2 and BAX...
May 12, 2017: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/28500853/protective-effects-of-circulating-microvesicles-derived-from-myocardial-ischemic-rats-on-apoptosis-of-cardiomyocytes-in-myocardial-ischemia-reperfusion-injury
#5
Yao Wang, Su Wei, Yi-Lu Wang, Miao Liu, Man Shang, Qi Zhang, Yan-Na Wu, Ming-Lin Liu, Jun-Qiu Song, Yan-Xia Liu
OBJECTIVE: To investigate the effects of circulating microvesicles derived from myocardial ischemia (I-MVs) on apoptosis in myocardial ischemia/reperfusion (I/R) injury in rats. METHODS: I-MVs from rats undergoing myocardial left anterior descending (LAD) coronary artery ligation were isolated by ultracentrifugation from circulating blood and characterized by flow cytometry. I-MVs were administered intravenously (4.8 mg/kg) at 5 min before reperfusion procedure in I/R injury model which was induced by 30-min of ischemia and 120-min of reperfusion of LAD in rats...
April 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28498407/respiratory-syncytial-virus-infection-accelerates-lung-fibrosis-through-the-unfolded-protein-response-in-a-bleomycin-induced-pulmonary-fibrosis-animal-model
#6
Lina Wang, Wei Cheng, Zhimin Zhang
Emerging evidence has demonstrated that endoplasmic reticulum stress (ER) is involved in the pathogenesis of idiopathic pulmonary fibrosis, however, the underlying mechanism remains unclear. Viral infection often triggers a hyperinflammatory response by an expansion of the ER. The present study was designed to observe the role of respiratory syncytial virus infection (RSV)‑induced ER stress on lung fibrosis. In order to determine the role of ER stress on the onset and progression of pulmonary fibrosis, mice received an intratracheal combined injection of RSV and bleomycin on day 0...
May 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28497987/elevated-activating-transcription-factor-4-and-glucose-regulated-78-kda-protein-levels-correlate-with-inflammatory-cytokines-in-the-aqueous-humor-and-vitreous-of-proliferative-diabetic-retinopathy
#7
Yanuo Wang, Sha Gao, Yanji Zhu, Xi Shen
PURPOSE: To determine concentrations of endoplasmic reticulum (ER) stress-related factors activating transcription factor 4 (ATF4) and glucose-regulated 78 kDa protein (GRP78) in vitreous and aqueous humor (AqH) of patients with proliferative diabetic retinopathy (PDR) and the correlation of ATF4, GRP78 and inflammatory cytokines interleukin-6(IL-6) and monocyte chemoattractant protein-1 (MCP-1). MATERIALS AND METHODS: AqH and vitreous samples were collected from eyes of patients with PDR and idiopathic macular hole (IMH) which needed vitrectomy...
May 12, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28495142/n-methyl-d-aspartate-receptor-mediated-calcium-overload-and-endoplasmic-reticulum-stress-are-involved-in-interleukin-1beta-induced-neuronal-apoptosis-in-rat-hippocampus
#8
Yilong Dong, Allan V Kalueff, Cai Song
Increased levels of interleukin (IL)-1β and its gene expression are implicated in the etiology of Alzheimer's disease (AD). IL-1β activates microglia and stimulates glutamatergic N-methyl-d-aspartate receptor NMDA receptor expression, thereby disturbing intracellular Ca(2+) homeostasis. Ca(2+) disequilibrium, in turn, may trigger endoplasmic reticulum (ER) stress, contributing to overall excitotoxicity and neuronal death that evoke AD. However, it is unclear whether IL-1β-induced neuronal apoptosis is mediated by the glutamatergic system, ER stress and/or Ca(2+) dysfunction...
June 15, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/28493835/expression-and-release-of-glucose-regulated-protein-78-grp78-in-multiple-myeloma
#9
Normann Steiner, Bojana Borjan, Roman Hajek, Karin Jöhrer, Georg Göbel, Wolfgang Willenbacher, Johann Kern, Eberhard Gunsilius, Gerold Untergasser
INTRODUCTION: Multiple myeloma (MM) is a plasma cell neoplasm that is mostly incurable due to acquired resistance during the treatment course. Thus, we evaluated expression and release of glucose-regulated protein 78 kDa (GRP78/BiP), an endoplasmic reticulum (ER) based pro-survival chaperone involved in immunoglobulin folding and unfolded protein responses. RESULTS: GRP78 protein expression in the ER and on the cell surface did not significantly differ between MGUS, NDMM and RRMM patients although there was a trend to higher surface expression in RRMM...
April 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28488585/eets-reduces-lps-induced-hyperpermeability-by-targeting-grp78-mediated-src-activation-and-subsequent-rho-rock-signaling-pathway
#10
Ruolan Dong, Danli Hu, Yan Yang, Zhihui Chen, Menglu Fu, Dao Wen Wang, Xizhen Xu, Ling Tu
Integrity of endothelial barrier is a determinant of the prognosis in the acute lung injury caused by sepsis. The epoxyeicosatrienoic acids (EETs), metabolites of arachidonic acid, exhibit protective effects in various pathogenic states, however, whether EETs play a role in endothelial barrier enhancement and the involved mechanisms remain to be investigated. Here, we show that increased EETs level by endothelial specific cytochrome P450 epoxygenase 2J2 over-expression and soluble epoxide hydrolase (sEH) inhibitor TPPU reduced lipopolysaccharide-induced endothelial hyper-permeability in vivo, accompanied by improved survival of septic mice...
April 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28488455/intracellular-methylglyoxal-induces-oxidative-damage-to-pancreatic-beta-cell-line-ins-1-cell-through-ire1%C3%AE-jnk-and-mitochondrial-apoptotic-pathway
#11
Chongxiao Liu, Yanhong Huang, Yifan Zhang, Xueru Chen, Xiang Kong, Yan Dong
An increased intracellular methylglyoxal (MGO) under hyperglycemia led to pancreatic beta cell death. However, its mechanism in which way with MGO induced beta cell death remains unknown. We investigated both high glucose and MGO treatment significantly inclined intracellular MGO concentration and inhibited cell viability in vitro. MGO treatment also triggered intracellular advanced glycation end products (AGEs) formation, declined mitochondrial membrane potential (MMP), increased oxidative stress and the expression of ER stress mediators Grp78/Bip and p-PERK; activated mitochondrial apoptotic pathway, which could mimic by Glo1 knockdown...
May 10, 2017: Free Radical Research
https://www.readbyqxmd.com/read/28488286/steroidogenesis-decline-accompanied-with-reduced-antioxidation-and-endoplasmic-reticulum-stress-in-mice-testes-during-ageing
#12
D Huang, W Wei, F Xie, X Zhu, L Zheng, Z Lv
To gain an understanding of the mechanisms by which Leydig cell steroidogenic function degenerates with ageing, we explored steroidogenic gene expression in relation to antioxidation status and endoplasmic reticulum (ER) stress during the ageing of mice. Expression of StAR, P450scc and other steroidogenic enzymes decreased starting at middle age (12-month-old) compared to that of the young control (3-month-old) mice. The immunohistochemical staining intensity of 3β-HSD for Leydig cells was significantly weaker in the aged (24-month-old) group than that in the young control group...
May 9, 2017: Andrologia
https://www.readbyqxmd.com/read/28487027/interleukin-11-up-regulates-endoplasmic-reticulum-stress-induced-target-pdia4-in-human-first-trimester-placenta-and-in%C3%A2-vivo-in-mice
#13
A L Winship, K Sorby, J Correia, A Rainczuk, J Yap, E Dimitriadis
Interleukin (IL)11 is a crucial factor for human trophoblast function and placentation. Elevated levels are associated with pregnancy complications including preeclampsia, intrauterine growth restriction (IUGR) and preterm birth. However, the regulation of IL11 in the placenta has not been investigated. We examined the effect of pro-inflammatory cytokines IL1β and TNFα, as well as low oxygen tension (2%) on IL11 levels in first trimester placental villous explants. IL1β upregulated IL11 mRNA and protein, while TNFα and low oxygen had no effect...
May 2017: Placenta
https://www.readbyqxmd.com/read/28485890/inhibition-of-endoplasmic-reticulum-stress-apoptosis-by-estrogen-protects-human-umbilical-vein-endothelial-cells-through-the-pi3-kinase-akt-signaling-pathway
#14
Yu Wang, Xin Yang, Xiao-Dong Li, Yong-Fen Qi, Xiao-Jing He, Yan-Jie Wang
OBJECTIVES: We aimed to investigate whether the cardioprotective effect of estrogen is mediated by inhibiting the apoptosis induced by endoplasmic reticulum stress (ERS) and to explore the underlying signaling pathway responsible for this effect. METHODS: The effect of estrogen on ERS apoptosis, the mechanism responsible for that effect, and the ERS signaling pathways were examined in human umbilical vein endothelial cells (HUVECs) and measured using western blot or Hoechst stains...
May 9, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28484232/inhibition-of-sp1-prevents-er-homeostasis-and-causes-cell-death-by-lysosomal-membrane-permeabilization-in-pancreatic-cancer
#15
Patricia Dauer, Vineet K Gupta, Olivia McGinn, Alice Nomura, Nikita S Sharma, Nivedita Arora, Bhuwan Giri, Vikas Dudeja, Ashok K Saluja, Sulagna Banerjee
Endoplasmic reticulum (ER) stress initiates an important mechanism for cell adaptation and survival, named the unfolded protein response (UPR). Severe or chronic/prolonged UPR can breach the threshold for survival and lead to cell death. There is a fundamental gap in knowledge on the molecular mechanism of how chronic ER stress is stimulated and leads to cell death in pancreatic ductal adenocarcinoma (PDAC). Our study shows that downregulating specificity protein 1 (Sp1), a transcription factor that is overexpressed in pancreatic cancer, activates UPR and results in chronic ER stress...
May 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28482861/therapeutic-effect-of-urine-derived-stem-cells-for-protamine-lipopolysaccharide-induced-interstitial-cystitis-in-a-rat-model
#16
Jia Li, Hui Luo, Xingyou Dong, Qian Liu, Chao Wu, Teng Zhang, Xiaoyan Hu, Yuanyuan Zhang, Bo Song, Longkun Li
BACKGROUND: Interstitial cystitis (IC) is a chronic inflammation disorder mainly within the submucosal and muscular layers of the bladder. As the cause of IC remains unknown, no effective treatments are currently available. Administration of stem cell provides a potential for treatment of IC. METHODS: This study was conducted using urine-derived stem cells (USCs) for protamine/lipopolysaccharide (PS/LPS)-induced interstitial cystitis in a rodent model. In total, 60 female Sprague-Dawley rats were randomized into three experimental groups (n = 5/group): sham controls; IC model alone; and IC animals intravenously treated with USCs (1...
May 8, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28482410/-therapeutic-efficacy-and-mechanism-of-action-of-ginsenoside-rg1-in-treating-acute-hepatic-failure-in-mice
#17
H Luo, W X Huang, C Yang, J Q Zhao, S Liu, Y S Xu, C W Liu
Objective: To examine the regulatory effect of ginsenoside Rg1 (G-Rg1) on endoplasmic reticulum stress and its effect on hepatocellular apoptosis in carbon tetrachloride (CCl(4))-induced acute liver failure (ALF). Methods: Forty healthy, adult male C57/BL mice were randomly divided into normal saline control (NS) group, G-Rg1 blank control (G-Rg1) group, CCl(4) model (CCl(4)) group, and G-Rg1 preventive treatment (CCl(4)+G-Rg1) group, and an ALF mouse model was established by CCl(4) induction. Blood and liver specimens were collected from all mice upon sacrifice at 12 hours post-intraperitoneal injection...
March 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/28478799/role-of-microrna-124-in-cardiomyocyte-hypertrophy-inducedby-angiotensin-ii
#18
Q Bao, L Chen, J Li, M Zhao, S Wu, W Wu, X Liu
Cardiac hypertrophy is a crucial predictor of heart failure and is regulated by microRNAs. MicroRNA-124 (miR-124) is regarded as a prognostic indicator for outcomes after cardiac arrest. However, whether miR-124 participates in cardiac hypertrophy remains unclear. Therefore, our study aimed to determine the role of miR-124 in angiotensin II(AngII)-induced myocardial hypertrophy and the possible mechanism. Primary cultured rat neonatal cardiomyocytes(NCMs) were transfected with miR-124 mimics or inhibitor, followed by AngII stimulation...
April 29, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28472796/the-sodium-glucose-co-transporter-2-inhibitor-empagliflozin-protects-against-diabetic-cardiomyopathy-by-inhibition-of-the-endoplasmic-reticulum-stress-pathway
#19
Yang Zhou, Wei Wu
BACKGROUND/AIMS: This study aimed to determine whether or not the sodium-glucose co-transporter 2 inhibitor, empagliflozin (EMPA), can protect against diabetic cardiomyopathy (DCM) and to elucidate the related mechanism. METHODS: Rats were divided into the following four groups: a non-diabetic group; diabetic cardiomyopathy rats without EMPA treatment; and diabetic cardiomyopathy rats with EMPA treatment (low- and high-dose EMPA). Hemodynamic measurements were performed to evaluate left ventricular systolic and diastolic function...
May 4, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28472786/roles-of-the-exogenous-h2s-mediated-sr-a-signaling-pathway-in-renal-ischemia-reperfusion-injury-in-regulating-endoplasmic-reticulum-stress-induced-autophagy-in-a-rat-model
#20
Qing Ling, Xiao Yu, Tao Wang, Shao-Gang Wang, Zhang-Qun Ye, Ji-Hong Liu
OBJECTIVE: This study aims to explore the effects of the exogenous hydrogen sulfide (H2S)-mediated scavenger receptor A (SR-A) signaling pathway on renal ischemia/reperfusion injury (IRI) by regulating endoplasmic reticulum (ER) stress-induced autophagy in rats. METHODS: A total of 48 normal Sprague-Dawley (SD) rats and SR-A knockout rats were selected and divided into six groups (n = 8): wild-type (WT) + sham, WT + ischemia-reperfusion (I/R), WT + I/R + NaHS, SR-A-/- + sham, SR-A-/- + I/R and SR-A-/- + I/R + NaHS...
May 4, 2017: Cellular Physiology and Biochemistry
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