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Jie Lei, Lei Zhao, Yujing Zhang, Yanfeng Wu, Yanbo Liu
BACKGROUND/AIMS: The mechanisms by which high glucose (HG) results in podocyte damage remains unclear. We investigated the potential role of endoplasmic reticulum (ER) stress and mTOR signaling in HG injured podocyte. METHODS: In cultured mouse podocytes, cellular apoptosis was assessed using FITC-Annexin V and propidium iodide staining followed by flow cytometry analysis. Apoptosis-related proteins as well as the ER stress and the mTOR signals were evaluated using immunoblot assay...
March 15, 2018: Cellular Physiology and Biochemistry
Zhiquan Lv, Chong Liu, Meili Zhai, Qian Zhang, Jun Li, Fang Zheng, Mingqing Peng
BACKGROUND/AIMS: Lipopolysaccharide (LPS) pretreatment has a strong neuroprotective effect on cerebral ischaemia/reperfusion injury (IRI), but the mechanism has not been fully elucidated to date. This study investigated the effect of LPS pretreatment on the pathway mediated by endoplasmic reticulum (ER) stress-CCAAT/enhancer-binding protein- homologous protein (CHOP) and the role of this pathway on cerebral ischaemia/reperfusion (I/R)-induced inflammation and apoptosis. METHODS: Healthy male BALB/c mice were randomised into four groups as follows: sham operation group (sham group, n=30); LPS group (BALB/c mice treated with LPS, n=30); ischaemia/reperfusion group (I/R group, n=30) and I/R+LPS group (BALB/c mice treated with LPS before ischaemia, n=30)...
March 10, 2018: Cellular Physiology and Biochemistry
Shengye Liu, Long Zhou, Liyu Yang, Shuai Mu, Tao Fang, Qin Fu
Background: Prolonged and over-dosed administration of glucocorticoids results in more bone remodeling, leading to glucocorticoid-induced osteoporosis, which is primarily due to dysfunction and apoptosis of osteoblasts. The present study investigated the therapeutic effect and molecular mechanism of gastrodin, a natural bioactive compound isolated from the traditional Chinese herbal agent Gastrodia elata, on osteoblastic cells in vivo and in vitro . Materials and Methods: The anti-dexamethasone (DEX) effects of gastrodin on primary osteoblasts were measured by cell viability, flow cytometry, and western blot analysis in vitro , and also extensively examined in a rat model in vivo ...
February 20, 2018: Oncotarget
Dicson Sheeja Malar, Venkatesan Suryanarayanan, Mani Iyer Prasanth, Sanjeev Kumar Singh, Krishnaswamy Balamurugan, Kasi Pandima Devi
Amyloid beta (Aβ) formation is one of the neuropathological hallmarks of Alzheimer's disease (AD), which induces the generation of reactive oxygen species (ROS), further leading to the alteration of several signalling pathways. In the present study, vitexin has been evaluated for its neuroprotective activity against Aβ25-35 induced toxicity in Neuro-2a cells. Results of cell free studies indicated that vitexin significantly inhibited the aggregation of Aβ25-35 . Studies in Neuro-2a cells revealed that Aβ25-35 significantly affected the cell viability by inducing ROS mediated toxicity and apoptosis...
March 12, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Yeong-Min Yoo, Eui-Bae Jeung
The phenotypes of calbindin-D9k - ( CaBP-9k -) knockout (KO), calbindin - D28k - ( CaBP-28k -) KO, and CaBP-9k/28k -KO mice are similar to those of wild-type (WT) mice due to the compensatory action of other calcium transport proteins. In this study, we investigated the expression of cellular prion protein (PrPC ) in the brains of CaBP-9k -, CaBP-28k -, and CaBP-9k/28k -KO mice. PrPC expression was significantly upregulated in the brain of all three strains. Levels of phospho-Akt (Ser473) and phospho-Bad (Ser136) were significantly elevated, but those of phospho-ERK and phospho-Bad (Ser155 and 112) were significantly reduced in the brains of CaBP-9k -, CaBP-28k -, and CaBP-9k/28k -KO mice...
2018: Oxidative Medicine and Cellular Longevity
Wen-Jun Yan, Ruo-Bin Liu, Ling-Kai Wang, Ya-Bing Ma, Shao-Li Ding, Fei Deng, Zhong-Yuan Hu, Da-Bin Wang
Endoplasmic reticulum (ER) stress occurring in stringent conditions is critically involved in neuronal survival and death. Resveratrol is a non-flavonoid polyphenol that has neuroprotective effects against many neurological disorders. Here, we investigated the potential protective effects of resveratrol in an in vitro ER stress model mimicked by tunicamycin (TM) treatment in neuronal HT22 cells. We found that TM dose-dependently decreased cell viability and increased apoptosis, which were both significantly attenuated by resveratrol treatment...
2018: Frontiers in Neuroscience
Denisse Sepúlveda, María-José Barrera, Isabel Castro, Sergio Aguilera, Patricia Carvajal, Carolina Lagos, Sergio González, Nicolás Albornoz, Verónica Bahamondes, Andrew F G Quest, Ulises Urzúa, Claudio Molina, Cecilia Leyton, Marcela A Hermoso, María-Julieta González
Objectives: Labial salivary glands (LSGs) of SS patients show alterations related to endoplasmic reticulum stress. Glandular dysfunction could be partly the consequence of an altered inositol-requiring enzyme 1α (IRE1α)/X box-binding protein 1 (XBP-1) signalling pathway of the unfolded protein response, which then regulates genes involved in biogenesis of the secretory machinery. This study aimed to determine the expression, promoter methylation and localization of the IRE1α/XBP-1 pathway components in LSGs of SS patients and also their expression induced by IFN-γ in vitro...
March 9, 2018: Rheumatology
Xiaohua Wang, Cheng Chen, Guoren Zhou, Jinjun Ye, Rong Yin, Dongjie Feng, Shuai Zhang, Xiaojun Wang, Xin Zhao, Zhi Zhang
BACKGROUND/AIMS: Our previous study suggested the anti-tumor activity of sepia ink oligopeptide (SIO). Here we sought to investigate the underlying molecular mechanism. METHODS: Cell proliferation was evaluated by cell counting kit-8 (CCK-8) assay. Cell apoptosis was determined by Annexin V/Propidium Iodide (PI) staining. The mitochondria pathway was characterized by quantification of Bcl-2, Bax, Caspase-9 and Cyto-C. The death receptor pathway was analyzed by determinement of Fas, Caspase-8 and NIK...
March 7, 2018: Cellular Physiology and Biochemistry
Shan Yang, You-Chen Zhang, Hui-Wen Li, Zheng-Yong Jin
OBJECTIVE: To investigate the protective effect of prostaglandin E1 (PGE-1) against brain injury induced by hyperoxia in neonatal rats and observe the changes in the expression of glucose-regulated protein 78 (GRP78) and cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP), and to provide a theoretical basis for the clinical application of PGE-1 in the treatment of neonatal brain injury induced by hyperoxia. METHODS: Sixty neonatal Wistar rats were randomly divided into air control group, hyperoxic brain injury model group, and hyperoxic brain injury+PGE-1 group...
March 2018: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
Mitchell R K L Lie, Janine van der Giessen, Gwenny M Fuhler, Alison de Lima, Maikel P Peppelenbosch, Cokkie van der Ent, C Janneke van der Woude
BACKGROUND: Around 30% of patients with inflammatory bowel disease (IBD) are refractory to current IBD drugs or relapse over time. Novel treatments are called for, and low dose Naltrexone (LDN) may provide a safe, easily accessible alternative treatment option for these patients. We investigated the potential of LDN to induce clinical response in therapy refractory IBD patients, and investigated its direct effects on epithelial barrier function. METHODS: Patients not in remission and not responding to conventional therapy were offered to initiate LDN as a concomitant treatment...
March 9, 2018: Journal of Translational Medicine
N Zhao, L Mi, X J Zhang, M Y Xu, H Y Yu, Z W Liu, X J Liu, G C Guan, W Gao, J K Wang
MicroRNA 711 (miR-711) levels in the heart change dynamically after myocardial infarction (MI). As peroxisome proliferator-activated receptor gamma (PPARγ) can upregulate miR-711 in adipocytes and cardiac fibroblasts, this study examined the precise mechanism of PPARγ-mediated miR-711 upregulation and its role in the heart in the early stages after MI. In a rat model of MI induced by left anterior descending coronary artery ligation, immunohistochemical and western blot analyses revealed increased PPARγ expression in cardiomyocyte nuclei after MI...
March 6, 2018: Journal of Molecular and Cellular Cardiology
Hongyan Wu, Qing Chang
Valvular diseases are common health problems that are strongly related to high morbidity and mortality; aortic valve allograft transplantation may be a promising way to improve survival and relieve symptoms. However, ideal tissue viability has not been observed with common valve cryopreservation methods, which could lead to apoptosis and necrosis in cryopreserved tissue. It has been observed that trehalose plays a positive role by acting to maintain cell structures and protect cells from stress responses. In this study, we studied the effects of trehalose in protecting rat valve tissue from the cooling process...
2018: PloS One
Q Zhang, J L Huang, Y B Liang, Y P He, D W Tong, X G Xu
Transmissible gastroenteritis virus (TGEV) is an important pathogen in swine that is responsible for substantial economic losses. Previous studies suggest that the TGEV non-structural protein 7 (nsp7) plays an important role in the viral assembly process. However, the subcellular localization and other functions of the TGEV nsp7 protein are still unclear. In this study we have examined the subcellular localization and other functions of TGEV nsp7 protein through analysis of its effects on cell growth, cell cycle progression, interleukin 8 (IL-8) expression, and NF-κB activation...
2018: Acta Virologica
Ri-Zhen Huang, Xiao-Chao Huang, Bin Zhang, Hai-Yang Jia, Zhi-Xin Liao, Heng-Shan Wang
BACKGROUND: Hepatocellular carcinoma (HCC) is the leading cause of cancer death, and novel chemotherapeutic drugs for treating HCC are urgently needed. 16-O-caffeoyl-16-hydroxylhexadecanoic acid (CHHA) is a new phenylpropanoid isolated by our group from Euphorbia nematocypha which is commonly used to treat solid tumors. However, the underlying mechanisms responsible for the CHHA-induced apoptosis in cancer cells, particularly in HCC, remain unknown. PURPOSE: In the present work, we evaluated the growth inhibitory effect of CHHA on HCC cells and explored the underlying molecular mechanisms...
March 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Haibo Ni, Qin Rui, Yitian Xu, Jun Zhu, Fan Gao, Baoqi Dang, Di Li, Rong Gao, Gang Chen
Receptor for activated protein kinase C 1 (RACK1) is a multifaceted scaffolding protein known to be involved in the regulation of signaling events required for neuronal protection. In the present study, we investigated the role of RACK1 in secondary brain injury in a rat traumatic brain injury (TBI) model. A weight-drop TBI model was established in Sprague Dawley rats, and RACK1 in vivo knockdown and overexpression were performed 24 h before TBI insult. The IRE1 inhibitor 3,5-dibromosalicylaldehyde (DBSA) was administered by intracerebroventricular injection 1 h after TBI insult...
March 5, 2018: Experimental Neurology
Eran Gershon, Ron Hadas, Michal Elbaz, Evan Booker, Moran Muchnik, Alona Kleinjan, Sharon Karasenti, Olga Genin, Yuval Cinnamon, Peter C Gray
Cripto-1 (TDGF1) is a multifunctional signaling factor that stimulates cellular effects including proliferation, migration, survival, EMT and angiogenesis to regulate embryogenesis, tissue homeostasis and tumorigenesis. Those cell behaviors are also associated with implantation of the embryo into the uterine wall and this led us to investigate the role of embryo-derived Cripto in embryo attachment and implantation. We show here that Cripto and its signaling mediator GRP78 are uniquely localized to embryo implantation sites...
February 28, 2018: Endocrinology
Haowen Dou, Shasha Yang, Yulai Hu, Dongyuan Xu, Lan Liu, Xiangdan Li
AIMS: Sesamin, a major lignan of sesame oil, has demonstrated anticancer properties. However, its anticancer effects on cervical cancer have not been studied. Here, we investigated the effects of sesamin on cervical cancer (HeLa) cell line and explored the underlying mechanisms. MAIN METHODS: HeLa cells were cultured with sesamin. CCK-8 and scratch wound test were applied to detect the proliferation and migration ability, while flow cytometry and TUNEL staining were applied to detect apoptosis...
March 2, 2018: Life Sciences
Ömer Hazman, Mehmet Fatih Bozkurt, Abdurrahman Fatih Fidan, Fadime Erkan Uysal, Sefa Çelik
The development of treatment protocols that can reduce side effects in chemotherapy applications is extremely important in terms of cancer treatment. In this context, it was aimed to investigate the effects of boric acid and borax on cisplatin toxicity (nephrotoxicity) in rats. In the experimental phase, eight groups were formed from rats. Boric acid and borax were given to the treatment groups with three different doses using gavage. On the fifth day of the study, cisplatin (10 mg/kg) was administered to all rats except the control group...
March 2, 2018: Inflammation
Yiqun Bian, Hao Wang, Shui Sun
Osteoarthritis (OA), characterized by pain and stiffness, swelling, deformity and dysfunction of joints, affects large numbers of population. The purpose of this study was to discover the effects of taurine in human OA chondrocytes and explore the underlying mechanisms. 46 patients with different grades of OA were recruited. Of these patients, 24 underwent total knee replacement and cartilages were harvested. The mRNA expressions of type II collagen (Collagen II) and endoplasmic reticulum (ER) stress markers (GRP78, GADD153 and Caspase-12) in cartilages were quantified by qRT-PCR...
December 2018: Redox Report: Communications in Free Radical Research
Wensheng Liu, Kaizhong Liu, Shu Zhang, Lihong Shan, Jiangfeng Tang
BACKGROUND Acute lung injury (ALI) is a life-threatening complication of sepsis. Tetramethylpyrazine (TMP) has been used in the clinical treatment of vascular diseases. The aim of this study was to investigate the therapeutic effects and possible involved mechanisms on ALI. MATERIAL AND METHODS Cecal ligation and puncture (CLP) was used to establish a sepsis model in rats. TMP at various dosages were administrated to rats using a intragastric method. Animal survival rate was calculated. The lung functions were evaluated by lung weight/dry weight ratio (W/D), PaO2, dynamic compliance (DC), and airway resistance index (ARI)...
February 28, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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