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Na k atpase

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https://www.readbyqxmd.com/read/29155857/impaired-aqp2-trafficking-in-fxyd1-knockout-mice-a-role-for-fxyd1-in-regulated-vesicular-transport
#1
Elena Arystarkhova, Richard Bouley, Yi Bessie Liu, Kathleen J Sweadner
The final adjustment of urine volume occurs in the inner medullary collecting duct (IMCD), chiefly mediated by the water channel aquaporin 2 (AQP2). With vasopressin stimulation, AQP2 accumulation in the apical plasma membrane of principal cells allows water reabsorption from the lumen. We report that FXYD1 (phospholemman), better known as a regulator of Na,K-ATPase, has a role in AQP2 trafficking. Daytime urine of Fxyd1 knockout mice was more dilute than WT despite similar serum vasopressin, but both genotypes could concentrate urine during water deprivation...
2017: PloS One
https://www.readbyqxmd.com/read/29154223/mitochondrial-f1-atpase-extends-glycolysis-and-ph-decline-in-an-in-vitro-model
#2
Sulaiman K Matarneh, Mariane Beline, Saulo de Luz E Silva, Hao Shi, David E Gerrard
The experiment was conducted to identify the mitochondrial protein responsible for enhancing glycolytic flux. We hypothesized that mitochondrial F1-ATPase promotes ATP hydrolysis and thereby the flux through glycolysis. Porcine longissimus muscle mitochondria were incorporated into an in vitro system designed to recapitulate postmortem glycolysis with or without Na-azide to specifically inhibit the β-subunit of mitochondrial F1-ATPase that catalyzes ATP hydrolysis. Addition of mitochondria enhanced ATP hydrolysis, glycogen degradation, lactate accumulation, and pH decline in the in vitro system...
November 7, 2017: Meat Science
https://www.readbyqxmd.com/read/29152793/effects-of-dietary-tryptophan-levels-on-performance-and-biochemical-variables-of-plasma-and-intestinal-mucosa-in-yellow-feathered-broiler-breeders
#3
S Q Jiang, Z Y Gou, X J Lin, L Li
The effects of dietary tryptophan (Trp) levels on performance and biochemical variables of plasma and intestinal mucosa in broiler breeder hens were investigated in this study. A total of 780 Lingnan yellow-feathered broiler breeder hens were randomly assigned in one of five dietary treatments with six replicates per treatment (26 birds per replicate). The breeder hens were fed either the basal diet (0.11% Trp) or the basal diet supplemented to 0.15%, 0.19%, 0.23% and 0.27% Trp, from 197 to 259 days of age...
November 19, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29148540/single-cell-heterogeneity-in-ductal-carcinoma-in-situ-of-breast
#4
Michael J Gerdes, Yesim Gökmen-Polar, Yunxia Sui, Alberto Santamaria Pang, Nicole LaPlante, Adrian L Harris, Puay-Hoon Tan, Fiona Ginty, Sunil S Badve
Heterogeneous patterns of mutations and RNA expression have been well documented in invasive cancers. However, technological challenges have limited the ability to study heterogeneity of protein expression. This is particularly true for pre-invasive lesions such as ductal carcinoma in situ of the breast. Cell-level heterogeneity in ductal carcinoma in situ was analyzed in a single 5 μm tissue section using a multiplexed immunofluorescence analysis of 11 disease-related markers (EGFR, HER2, HER4, S6, pmTOR, CD44v6, SLC7A5 and CD10, CD4, CD8 and CD20, plus pan-cytokeratin, pan-cadherin, DAPI, and Na+K+ATPase for cell segmentation)...
November 17, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29146150/the-effect-of-cold-acclimation-on-active-ion-transport-in-cricket-ionoregulatory-tissues
#5
Lauren E Des Marteaux, Soheila Khazraeenia, Gil Y Yerushalmi, Andrew Donini, Natalia G Li, Brent J Sinclair
Cold-acclimated insects defend ion and water transport function during cold exposure. We hypothesized that this is achieved via enhanced active transport. The Malpighian tubules and rectum are likely targets for such transport modifications, and recent transcriptomic studies indicate shifts in Na(+)-K(+) ATPase (NKA) and V-ATPase expression in these tissues following cold acclimation. Here we quantify the effect of cold acclimation (one week at 12°C) on active transport in the ionoregulatory organs of adult Gryllus pennsylvanicus field crickets...
November 13, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/29137423/expression-of-the-%C3%AE-3-subunit-of-na-k-atpase-is-increased-in-gastric-cancer-and-regulates-gastric-cancer-cell-progression-and-prognosis-via-the-pi3-akt-pathway
#6
Li Li, Ru Feng, Qian Xu, Feiyue Zhang, Tong Liu, Jiang Cao, Sujuan Fei
ATP1B3 encodes the β3 subunit of Na(+)/K(+)-ATPase and is located in the q22-23 region of chromosome 3. Na(+)/K(+)-ATPase participates in normal cellular activities but also plays a crucial role in carcinogenesis. In the present study, we found that expression of the β3 subunit of Na(+)/K(+)-ATPase was increased in human gastric cancer tissues compared with that in normal matched tissues and that this increased expression predicted a poor outcome. ATP1B3 expression was elevated at both the mRNA and protein levels in gastric cancer cell lines relative to those in a normal gastric epithelial cell line...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29136770/magnesium-valproate-ameliorates-type-1-diabetes-and-cardiomyopathy-in-diabetic-rats-through-estrogen-receptors
#7
Samir Rabadiya, Shradhha Bhadada, Ashvin Dudhrejiya, Devendra Vaishnav, Bhoomika Patel
Estrogen is known to exhibit cardioprotective and antihyperlipidemic action. Valproic acid has been shown to upregulate estrogen receptors (ERs) in breast and prostate cancer tissues. No pharmacological evaluations for magnesium valproate (MgV) so far have been done for diabetic cadio-lipidemic complications. Based on the above context, current study was undertaken to evaluate the therapeutic effectiveness of MgV in cardiac complications associated with type-1 diabetes mellitus in rats wherein diabetes was induced by single tail vein injection of streptozotocin (STZ, 45mg/kg, IV) in female Sprague Dawley rats and treatment of MgV (210mg/kg, PO) was given for eight weeks to diabetic animals, after which, various biochemical and cardiac biomarkers, hypertrophic, hemodynamic and histological parameters along with immunohistochemistry of ERs in the left ventricle (LV) were estimated...
November 7, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29133296/comparison-of-preservation-and-transportation-protocols-for-preloaded-descemet-membrane-endothelial-keratoplasty
#8
Vito Romano, Mohit Parekh, Alessandro Ruzza, Colin E Willoughby, Stefano Ferrari, Diego Ponzin, Stephen B Kaye, Hannah J Levis
BACKGROUND/AIMS: Descemet membrane endothelial keratoplasty (DMEK) preparation is technically demanding and is a limiting factor for uptake of this kind of surgery. Supply methods that simplify the procedure for surgeons are key to increasing uptake. This study compares two different shipping protocols for DMEK. METHODS: An 8.5 mm DMEK graft was punched, marked and loaded for transportation in two different conditions: (A) endothelium trifolded inwards in organ culture conditions (n=7) and (B) endothelium rolled outwards in hypothermic conditions (n=7)...
November 13, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29130514/involvement-of-src-signaling-in-the-synergistic-effect-between-cisplatin-and-digoxin-on-cancer-cell-viability
#9
Duane Gischewski Pereira, Mariana Alves Resende Salgado, Sayonarah Carvalho Rocha, Hérica L Santos, José Augusto Fp Villar, Ruben G Contreras, Carlos Frederico Leite Fontes, Leandro Augusto Barbosa, Vanessa Faria Cortes
Cisplatin and other platinum-containing drugs have played a crucial role in anticancer treatments for over 30 years. However, treatment with cisplatin may cause serious side effects, such as myelosuppression, nausea, ototoxicity, nephrotoxicity and cell resistance processes. In addition, cardiotonic steroids, particularly digoxin, have recently been suggested to exert potent anticancer effects. Therefore, it is possible that the combined treatment of HeLa cells with cisplatin and digoxin can ameliorate the cytotoxic effects and decrease the side effects of cisplatin...
November 11, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29129208/augmented-gene-expression-triggered-by-na-k-atpase-inhibition-role-of-ca-2-i-mediated-and-independent-excitation-transcription-coupling
#10
Larisa V Smolyaninova, Svetlana V Koltsova, Svetlana V Sidorenko, Sergei N Orlov
In rat vascular smooth muscle cells (RVSMC), 3-h Na(+),K(+)-ATPase inhibition by ouabain or in K(+)-free medium resulted in the inversion of the [Na(+)]i/[K(+)]i ratio and elevation up to 7-fold the content of Egr1, Atf3, Nr4a1 and Ptgs2 mRNAs. Ouabain increased the rate of 45Ca(2+) influx by 2-fold that was abolished by L-type voltage-gated Ca(2+) channel blocker nicardipine, but it was resistant to Na(+)/Ca(2+) exchanger inhibitor KB-R7943. To study the role of Ca(2+)-mediated signaling in the expression of Na(+)i/K(+)i-sensitive genes we used intracellular Ca(2+) chelator BAPTA and incubated RVSMC in Ca(2+)-free medium...
December 2017: Cell Calcium
https://www.readbyqxmd.com/read/29124681/synergistic-toxicity-of-the-neurometabolites-quinolinic-acid-and-homocysteine-in-cortical-neurons-and-astrocytes-implications-in-alzheimer-s-disease
#11
Paula Pierozan, Helena Biasibetti-Brendler, Felipe Schmitz, Fernanda Ferreira, Carlos Alexandre Netto, Angela T S Wyse
The brain of patients affected by Alzheimer's disease (AD) develops progressive neurodegeneration linked to the formation of proteins aggregates. However, their single actions cannot explain the extent of brain damage observed in this disorder, and the characterization of co-adjuvant involved in the early toxic processes evoked in AD is essential. In this line, quinolinic acid (QUIN) and homocysteine (Hcy) appear to be involved in the AD neuropathogenesis. Herein, we investigate the effects of QUIN and Hcy on early toxic events in cortical neurons and astrocytes...
November 9, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/29120676/glutamine-protection-in-an-experimental-model-of-acetaminophen-nephrotoxicity
#12
Marco Brovedan, Sara María Molinas, Gerardo Pisani, Liliana Alicia Monasterolo, Laura Trumper
Acetaminophen (APAP) is a widely prescribed analgesic - antipyretic drug. In the present work we studied the effects of glutamine (Gln) in an "in vivo" model of APAP-induced nephrotoxicity in male Wistar rats. Renal function, histological characteristics and Na+, K+ ATPase cortical abundance and distribution were analyzed. The appearance of HSP70 and actin in urine were also evaluated. Mieloperoxidase activity in cortical tissue was measured as an index of the inflammatory response. Gln administration 30 min before APAP protected from the renal functional and histological damage promoted by APAP...
November 9, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/29120088/assessment-of-chemotherapy-on-various-biochemical-markers-in-breast-cancer-patients
#13
Márcia Fernanda Correia Jardim Paz, Antonio Luiz Gomes Júnior, Muhammad Torequl Islam, Shams Tabrez, Nasimudeen R Jabir, Mohammad Zubair Alam, Kátia da Conceição Machado, Marcus Vinícius Oliveira Barros de Alencar, Keylla da Conceição Machado, Eunus S Ali, Siddhartha Kumar Mishra, Leonardo Fortes Gomes, João Marcelo de Castro E Sousa, Geane Felix de Souza, Ana Amélia de Carvalho Melo-Cavalcante, Juliana da Silva
Chemotherapy is a standard treatment method for the patients with locally advanced breast cancer. Lately, cyclophosphamide (CYP) and doxorubicin (DOX) are used as the major chemotherapeutic agents. Till date, no serum biomarker has been able to provide an early diagnosis of breast cancer. This study aimed to assess inflammatory, cardiac, renal and hematological markers in 56 breast cancer patients (BCP) before, during and after termination of chemotherapy with CYP and DOX. Blood samples were collected from the patients at the each treatment stages mentioned above...
November 9, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29119246/skeletal-muscle-and-performance-adaptations-to-high-intensity-training-in-elite-male-soccer-players-speed-endurance-runs-versus-small-sided-game-training
#14
Dan Fransson, Tobias Schmidt Nielsen, Karl Olsson, Tobias Christensson, Paul S Bradley, Ioannis G Fatouros, Peter Krustrup, Nikolai Baastrup Nordsborg, Magni Mohr
PURPOSE: To examine the skeletal muscle and performance responses across two different exercise training modalities which are highly applied in soccer training. METHODS: Using an RCT design, 39 well-trained male soccer players were randomized into either a speed endurance training (SET; n = 21) or a small-sided game group (SSG; n = 18). Over 4 weeks, thrice weekly, SET performed 6-10 × 30-s all-out runs with 3-min recovery, while SSG completed 2 × 7-9-min small-sided games with 2-min recovery...
November 8, 2017: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/29118925/bardoxolone-methyl-cddo-me-or-rta402-induces-cell-cycle-arrest-apoptosis-and-autophagy-via-pi3k-akt-mtor-and-p38-mapk-erk1-2-signaling-pathways-in-k562-cells
#15
Xin-Yu Wang, Xue-Hong Zhang, Li Peng, Zheng Liu, Yin-Xue Yang, Zhi-Xu He, Hong-Wan Dang, Shu-Feng Zhou
Chronic myeloid leukemia (CML) treatment remains a challenge due to drug resistance and severe side effect, rendering the need on the development of novel therapeutics. CDDO-Me (Bardoxolone methyl), a potent Nrf2 activator and NF-κB inhibitor, is a promising candidate for cancer treatment including leukemia. However, the underlying mechanism for CDDO-Me in CML treatment is unclear. This study aimed to evaluate the molecular interactome of CDDO-Me in K562 cells using the quantitative proteomics approach stable-isotope labeling by amino acids in cell culture (SILAC) and explore the underlying mechanisms using cell-based functional assays...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29118027/heterogeneity-of-signal-transduction-by-na-k-atpase-%C3%AE-isoforms-role-of-src-interaction
#16
Hui Yu, Xiaoyu Cui, Jue Zhang, Joe X Xie, Moumita Banerjee, Sandrine V Pierre, Zijian Xie
Of the four Na/K-ATPase α isoforms, the ubiquitous α1 Na/K-ATPase possesses both ion transport and Src-dependent signaling functions. Mechanistically, we have identified two putative pairs of domain interactions between α1 Na/K-ATPase and Src that are critical for α1 signaling. Our subsequent report that α2 Na/K-ATPase lacks these putative Src-binding sites and fails to carry on Src-dependent signaling further supported our proposed model of direct interaction between α1 Na/K-ATPase and Src, but fell short of providing evidence for a causative role...
November 8, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29117147/the-na-k-atpase-%C3%AE-subunit-isoforms-expression-in-glioblastoma-multiforme-moonlighting-roles
#17
Deborah Rotoli, Mariana-Mayela Cejas, María-Del-Carmen Maeso, Natalia-Dolores Pérez-Rodríguez, Manuel Morales, Julio Ávila, Ali Mobasheri, Pablo Martín-Vasallo
Glioblastoma multiforme (GBM) is the most common form of malignant glioma. Recent studies point out that gliomas exploit ion channels and transporters, including Na, K-ATPase, to sustain their singular growth and invasion as they invade the brain parenchyma. Moreover, the different isoforms of the β-subunit of Na, K-ATPase have been implicated in regulating cellular dynamics, particularly during cancer progression. The aim of this study was to determine the Na, K-ATPase β subunit isoform subcellular expression patterns in all cell types responsible for microenvironment heterogeneity of GBM using immunohistochemical analysis...
November 8, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29117117/anticancer-and-immunogenic-properties-of-cardiac-glycosides
#18
REVIEW
Naira Fernanda Zanchett Schneider, Claudia Cerella, Cláudia Maria Oliveira Simões, Marc Diederich
Cardiac glycosides (CGs) are natural compounds widely used in the treatment of several cardiac conditions and more recently have been recognized as potential antitumor compounds. They are known to be ligands for Na/K-ATPase, which is a promising drug target in cancer. More recently, in addition to their antitumor effects, it has been suggested that CGs activate tumor-specific immune responses. This review summarizes the anticancer aspects of CGs as new strategies for immunotherapy and drug repositioning (new horizons for old players), and the possible new targets for CGs in cancer cells...
November 8, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29116460/potential-usefulness-of-methyl-gallate-in-the-treatment-of-experimental-colitis
#19
María Laura Anzoise, Angeles Rodríguez Basso, Julieta Sofía Del Mauro, Andrea Carranza, Graciela López Ordieres, Susana Gorzalczany
Methyl gallate is a gallotannin widely distributed in nature. Previous studies have demonstrated its antioxidant, anti-inflammatory, antimicrobial and anti-tumor activities. In the present study, the activity of methyl gallate on experimental models of inflammatory bowel disease has been investigated. Experimental colitis was induced in Sprague-Dawley rats through the intracolonic instillation of an acetic acid solution (2 mL, 4% v/v). Methyl gallate (100 and 300 mg/kg, p.o.) and the reference drug mesalazine (100 mg/kg, p...
November 7, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/29115894/selectivity-analyses-of-%C3%AE-benzylidene-digoxin-derivatives-to-different-na-k-atpase-%C3%AE-isoforms-a-molecular-docking-approach
#20
Marco T C Pessôa, Silmara L G Alves, Alex G Taranto, José A F P Villar, Gustavo Blanco, Leandro A Barbosa
Digoxin and other cardiotonic steroids (CTS) exert their effect by inhibiting Na,K-ATPase (NKA) activity. CTS bind to the various NKA isoforms that are expressed in different cell types, which gives CTS their narrow therapeutic index. We have synthesised a series of digoxin derivatives (γ-Benzylidene digoxin derivatives) with substitutions in the lactone ring (including non-oxygen and ether groups), to obtain CTS with better NKA isoform specificity. Some of these derivatives show some NKA isoform selective effects, with BD-3, BD-8, and BD-13 increasing NKA α2 activity, BD-5 inhibiting NKA α1 and NKA α3, BD-10 reducing NKA α1, but stimulating NKA α2 and α3; and BD-14, BD-15, and BD-16 enhancing NKA α3 activity...
December 2018: Journal of Enzyme Inhibition and Medicinal Chemistry
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