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Na k atpase

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https://www.readbyqxmd.com/read/28324828/evaluation-of-dna-damage-and-physiological-responses-in-nile-tilapia-oreochromis-niloticus-linnaeus-1758-exposed-to-sub-lethal-diclofenac-dcf
#1
Pramod K Pandey, Malachy N O Ajima, Kundan Kumar, Nalini Poojary, Saurav Kumar
The frequent bioaccumulation of pharmaceuticals in the aquatic ecosystem has raised a concern about their possible ecotoxicological consequences. DNA damage, haematological changes and activities of oxidative stress enzymes in Nile tilapia, Oreochromis niloticus in response to diclofenac (DCF) exposure were investigated for up to 60 days at the concentrations of 0.17, 0.34 and 0.68mgL(-1) in the fish liver. Evaluation of genotoxic effects of the drug in the liver, using single-cell gel electrophoresis, showed DNA damage on exposure at the concentrations of 0...
March 8, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28316890/computer-modelling-reveals-new-conformers-of-the-atp-binding-loop-of-na-k-atpase-involved-in-the-transphosphorylation-process-of-the-sodium-pump
#2
Gracian Tejral, Bruno Sopko, Alois Necas, Wilhelm Schoner, Evzen Amler
Hydrolysis of ATP by Na(+)/K(+)-ATPase, a P-Type ATPase, catalyzing active Na(+) and K(+) transport through cellular membranes leads transiently to a phosphorylation of its catalytical α-subunit. Surprisingly, three-dimensional molecular structure analysis of P-type ATPases reveals that binding of ATP to the N-domain connected by a hinge to the P-domain is much too far away from the Asp(369) to allow the transfer of ATP's terminal phosphate to its aspartyl-phosphorylation site. In order to get information for how the transfer of the γ-phosphate group of ATP to the Asp(369) is achieved, analogous molecular modeling of the M4-M5 loop of ATPase was performed using the crystal data of Na(+)/K(+)-ATPase of different species...
2017: PeerJ
https://www.readbyqxmd.com/read/28315791/neurotoxic-effects-molecular-responses-and-oxidative-stress-biomarkers-in-nile-tilapia-oreochromis-niloticus-linnaeus-1758-exposed-to-verapamil
#3
Malachy N O Ajima, Pramod K Pandey, Kundan Kumar, Nalini Poojary
Pharmaceutical drugs and their metabolites are detected in aquatic ecosystems and have been reported to cause ecotoxicological consequences to resident aquatic organisms The study investigated the effects of acute and long-term exposure to verapamil on activities of acetylcholinesterase and antioxidant enzymes as well as mRNA expression of stress-related genes in brain and muscle tissues of Nile tilapia, Oreochromis niloticus. The 96h LC50 of verapamil to O. niloticus was 2.29mg L(-1). Exposure to sub-lethal concentrations of verapamil (0...
March 15, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28301970/disclosing-the-parameters-leading-to-high-productivity-of-retroviral-producer-cells-lines-evaluating-random-vs-targeted-integration
#4
Vanessa Sofia Bandeira, Hélio A Tomás, Evren Alici, Manuel J C T Carrondo, Ana Sofia Coroadinha
Gammaretrovirus and lentivirus are the preferred viral vectors to genetically modify T- and NK- cells to be used in immune-cell therapies. The transduction efficiency of hematopoietic and T cells is more efficient using Gibbon ape leukemia virus (GaLV) pseudotyping. In this context gammaretroviral vector producer cells offer competitive higher titers than transient lentiviral vectors productions. The main aim of this work was to identify the key parameters governing GalV pseudotyped gammaretroviral vector productivity in stable producer cells using a retroviral vector expression cassette enabling positive (facilitating cell enrichment) and negative cell selection (allowing cell elimination)...
March 16, 2017: Human Gene Therapy Methods
https://www.readbyqxmd.com/read/28301304/neuroprotective-and-therapeutic-effect-of-caffeine-on-the-rat-model-of-parkinson-s-disease-induced-by-rotenone
#5
Yasser A Khadrawy, Ahmed M Salem, Karima A El-Shamy, Emad K Ahmed, Nevein N Fadl, Eman N Hosny
The present study aimed to investigate the protective and therapeutic effects of caffeine on rotenone-induced rat model of Parkinson's disease (PD). Rats were divided into control, PD model induced by rotenone (1.5 mg/kg intraperitoneally (i.p.) for 45 days), protected group injected with caffeine (30 mg/kg, i.p.) and rotenone for 45 days (during the development of PD model), and treated group injected with caffeine (30 mg/kg, i.p.) for 45 days after induction of PD model. The data revealed a state of oxidative and nitrosative stress in the midbrain and the striatum of animal model of PD as indicated from the increased lipid peroxidation and nitric oxide levels and the decreased reduced glutathione level and activities of glutathione-S-transferase and superoxide dismutase...
September 3, 2017: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/28300845/digoxin-induced-retinal-degeneration-depends-on-rhodopsin
#6
Britta Landfried, Marijana Samardzija, Maya Barben, Christian Schori, Katrin Klee, Federica Storti, Christian Grimm
Na,K-ATPases are energy consuming ion pumps that are required for maintaining ion homeostasis in most cells. In the retina, Na,K-ATPases are especially important to sustain the dark current in photoreceptor cells needed for rapid hyperpolarization of rods and cones in light. Cardiac glycosides like digoxin inhibit the activity of Na,K-ATPases by targeting their catalytic alpha subunits. This leads to a disturbed ion balance, which can affect cellular function and survival. Here we show that the treatment of wild-type mice with digoxin leads to severe retinal degeneration and loss of vision...
March 16, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28298638/pnaktide-attenuates-steatohepatitis-and-atherosclerosis-by-blocking-na-k-atpase-ros-amplification-in-c57bl6-and-apoe-knockout-mice-fed-a-western-diet
#7
Komal Sodhi, Krithika Srikanthan, Perrine Goguet-Rubio, Alexandra Nichols, Amrita Mallick, Athar Nawab, Rebecca Martin, Preeya T Shah, Muhammad Chaudhry, Saroj Sigdel, Mehiar El-Hamdani, Jiang Liu, Zijian Xie, Nader G Abraham, Joseph I Shapiro
We have previously reported that the α1 subunit of sodium potassium adenosine triphosphatase (Na/K-ATPase), acts as a receptor and an amplifier for reactive oxygen species, in addition to its distinct pumping function. On this background, we speculated that blockade of Na/K-ATPase-induced ROS amplification with a specific peptide, pNaKtide, might attenuate the development of steatohepatitis. To test this hypothesis, pNaKtide was administered to a murine model of NASH: the C57Bl6 mouse fed a "western" diet containing high amounts of fat and fructose...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28297727/new-knowledge-about-old-drugs-the-anti-inflammatory-properties-of-cardiac-glycosides
#8
Robert Fürst, Ilse Zündorf, Theo Dingermann
In the 19th century, cardio-active steroid glycosides, shortly cardiac glycosides, were scientifically established as drugs against heart failure. Their in vivo, cellular, and molecular actions as well as their predominant target, Na(+)-K(+)-ATPase, have been comprehensively investigated in the 20th century and the discovery of endogenous cardiac glycosides has fostered this research field. In the last years, however, results from clinical trials and meta-analyses have questioned their therapeutic value due to efficacy and safety issues...
March 15, 2017: Planta Medica
https://www.readbyqxmd.com/read/28293195/comparative-investigation-of-copper-tolerance-and-identification-of-putative-tolerance-related-genes-in-tardigrades
#9
Thomas L Hygum, Dannie Fobian, Maria Kamilari, Aslak Jørgensen, Morten Schiøtt, Martin Grosell, Nadja Møbjerg
Tardigrades are microscopic aquatic animals renowned for their tolerance toward extreme environmental conditions. The current study is the first to investigate their tolerance toward heavy metals and we present a novel tardigrade toxicant tolerance assay based on activity assessments as a measure of survival. Specifically, we compare tolerance toward copper in four species representing different evolutionary lineages, habitats and adaptation strategies, i.e., a marine heterotardigrade, Echiniscoides sigismundi, a limno-terrestrial heterotardigrade, Echiniscus testudo, a limno-terrestrial eutardigrade, Ramazzottius oberhaeuseri, and a marine eutardigrade, Halobiotus crispae...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28292827/the-glycoside-oleandrin-reduces-glioma-growth-with-direct-and-indirect-effects-on-tumor-cells
#10
Stefano Garofalo, Alfonso Grimaldi, Giuseppina Chece, Alessandra Porzia, Stefania Morrone, Fabrizio Mainiero, Giuseppina D'Alessandro, Vincenzo Esposito, Barbara Cortese, Silvia Di Angelantonio, Flavia Trettel, Cristina Limatola
Oleandrin is a glycoside that inhibits the ubiquitous enzyme Na(+)-K(+)-ATPase. In addition to its known effects on cardiac muscle, recent in vitro and in vivo evidence highlighted potential for anticancer properties of this compound. In this paper we evaluated for the first time the effect of oleandrin on brain tumors. To this aim mice were transplanted with human or murine glioma and analyzed for tumor progression upon oleandrin treatment. In both systems, oleandrin impaired glioma development, reduced tumor size, and inhibited cell proliferation...
March 14, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28283795/na-k-atpase-%C3%AE-1-subunit-associates-with-%C3%AE-1-subunit-modulating-a-higher-nka-in-hyposmotic-media-response-in-gills-of-euryhaline-milkfish-chanos-chanos
#11
Yau-Chung Hu, Keng-Fu Chu, Wen-Kai Yang, Tsung-Han Lee
The euryhaline milkfish (Chanos chanos) is a popular aquaculture species that can be cultured in fresh water, brackish water, or seawater in Southeast Asia. In gills of the milkfish, Na(+), K(+)-ATPase (i.e., NKA; sodium pump) responds to salinity challenges including changes in mRNA abundance, protein amount, and activity. The functional pump is composed of a heterodimeric protein complex composed of α- and β-subunits. Among the NKA genes, α1-β1 isozyme comprises the major form of NKA subunits in mammalian osmoregulatory organs; however, most studies on fish gills have focused on the α1 subunit and did not verify the α1-β1 isozyme...
March 10, 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/28280923/na-k-atpase-response-to-salinity-change-and-its-correlation-with-fxyd11-expression-in-anguilla-marmorata
#12
Fenfei Liang, Li Li, Guosong Zhang, Shaowu Yin, Xiaolu Wang, Peng Li, Yihe Jia, Yayuan Wang, Li Wang, Xiaojun Wang
The Na(+)/K(+)-ATPase (NKA) is a primary electrogenic protein that promotes ion transport in teleosts. FXYD11 is a putative regulatory subunit of the NKA pump. The regulation of Na (+)/K (+) -ATPase and FXYD11 is of critical importance for osmotic homeostasis. To investigate the changes of the two genes under different salinity environments, we first identified NKA (AmNKAα1) and FXYD11 (AmFXYD11) in Anguilla marmorata, and then evaluated the mRNA levels of NKA and FXYD11 as well as the activity of NKA in the gill and kidney at different timepoints (0, 1, 3, 6, 12, 24, 48, 72, 96, and 360 h) under three salinity conditions-0‰ (fresh water: FW), 10‰ (brackish water: BW), and 25‰ (seawater: SW)...
March 9, 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/28277329/sevoflurane-compared-with-isoflurane-minimizes-lung-damage-in-pulmonary-but-not-in-extrapulmonary-acute-respiratory-distress-syndrome-in-rats
#13
Mariana N Araújo, Cíntia L Santos, Cynthia S Samary, Luciana B B Heil, Vinicius C M Cavalcanti, Fernanda F Cruz, Nathane S Felix, Johnatas D Silva, Marcelo M Morales, Paolo Pelosi, Fatima C Fernandes, Nivaldo R Villela, Pedro L Silva, Patricia R M Rocco
BACKGROUND: Volatile anesthetics modulate inflammation in acute respiratory distress syndrome (ARDS). However, it is unclear whether they act differently depending on ARDS etiology. We hypothesized that the in vivo and in vitro effects of sevoflurane and isoflurane on lung damage would not differ in pulmonary (p) and extrapulmonary (exp) ARDS. METHODS: Twenty-four Wistar rats were randomized to undergo general anesthesia (1-2 minutes) with sevoflurane and isoflurane...
March 8, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28276710/effects-of-vanillin-on-potassium-bromate-induced-neurotoxicity-in-adult-mice-impact-on-behavior-oxidative-stress-genes-expression-inflammation-and-fatty-acid-composition
#14
Hajer Ben Saad, Nadia Kharrat, Dorra Driss, Manel Gargouri, Rim Marrakchi, Kamel Jammoussi, Christian Magné, Tahia Boudawara, Samia Ellouz Chaabouni, Khaled Mounir Zeghal, Ahmed Hakim, Ibtissem Ben Amara
CONTEXT: Vanillin is known to possess important antioxidant activity. OBJECTIVE: The current study was conducted to establish the therapeutic efficiency of vanillin against potassium bromate (KBrO3)-induced depression-like behavior and oxidative stress in mice. MATERIAL AND METHODS: Mice were exposed during 15 days either to potassium bromate (KBrO3), KBrO3+ vanillin or to only vanillin. RESULTS: Our results revealed a significant modification in the fatty acid composition of the KBrO3-treated mice...
February 26, 2017: Archives of Physiology and Biochemistry
https://www.readbyqxmd.com/read/28274925/salt-supplementation-ameliorates-developmental-kidney-defects-in-cox-2-mice
#15
Patrick Slattery, Stefanie Frölich, Itamar Goren, Rolf Michael Nüsing
Deficiency of cyclooxygenase-2 (COX-2) activity in the early postnatal time course causes impairment of kidney development leading to kidney insufficiency. We hypothesize that impaired NaCl reabsorption during the first days of life is a substantial cause for nephrogenic defects observed in COX-2-/- mice and that salt supplementation corrects these defects. Daily injections of 0.8 mg/g/d NaCl for the first 10 days after birth ameliorated impaired kidney development in COX-2-/- pups resulting in an increase in glomerular size and reduction of immature superficial glomeruli...
March 8, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28274922/membrane-lipid-rafts-disturbance-in-the-response-of-rat-skeletal-muscle-to-short-term-disuse
#16
Alexey M Petrov, Violetta V Kravtsova, Vladimir V Matchkov, Alexander N Vasiliev, Andrey L Zefirov, Alexander V Chibalin, Judith A Heiny, Igor I Krivoi
Marked loss of skeletal muscle mass occurs under various conditions of disuse, but the molecular and cellular mechanisms leading to atrophy are not completely understood. We investigate early molecular events which might play a role in skeletal muscle remodeling during mechanical unloading (disuse). The effects of acute (6 - 12 h) hindlimb suspension on the soleus muscles from adult rats were examined. The integrity of plasma membrane lipid rafts was tested utilizing cholera toxin B subunit, or fluorescent sterols...
March 8, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28271418/blood-pressure-and-the-renal-actions-of-at2-receptors
#17
REVIEW
Robert M Carey
Angiotensin type-2 receptors (AT2Rs) in the renal proximal tubule inhibit sodium (Na+) reabsorption by inducing renal cyclic GMP formation and internalizing and inhibiting major Na+ transporters Na+-H+ exchanger-3 (NHE-3) and Na+/K+ATPase (NKA). Instead of angiotensin II (Ang II), angiotensin III (Ang III) is the predominant endogenous agonist for this response. Exogenous non-peptide AT2R agonist Compound-21 induces natriuresis and lowers blood pressure (BP) in normal and Ang II-infused hypertensive rodents...
March 2017: Current Hypertension Reports
https://www.readbyqxmd.com/read/28260988/human-corneal-endothelial-cells-expanded-in-vitro-are-a-powerful-resource-for-tissue-engineering
#18
REVIEW
Yongsong Liu, Hong Sun, Min Hu, Min Zhu, Sean Tighe, Shuangling Chen, Yuan Zhang, Chenwei Su, Subo Cai, Ping Guo
Human corneal endothelial cells have two major functions: barrier function mediated by proteins such as ZO-1 and pump function mediated by Na-K-ATPase which help to maintain visual function. However, human corneal endothelial cells are notorious for their limited proliferative capability in vivo and are therefore prone to corneal endothelial dysfunction that eventually may lead to blindness. At present, the only method to cure corneal endothelial dysfunction is by transplantation of a cadaver donor cornea with normal corneal endothelial cells...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28257114/na-k-atpase-signaling-and-salt-sensitivity-the-role-of-oxidative-stress
#19
REVIEW
Jiang Liu, Yanling Yan, Ying Nie, Joseph I Shapiro
Other than genetic regulation of salt sensitivity of blood pressure, many factors have been shown to regulate renal sodium handling which contributes to long-term blood pressure regulation and have been extensively reviewed. Here we present our progress on the Na/K-ATPase signaling mediated sodium reabsorption in renal proximal tubules, from cardiotonic steroids-mediated to reactive oxygen species (ROS)-mediated Na/K-ATPase signaling that contributes to experimental salt sensitivity.
March 2, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28256112/the-effects-of-altered-membrane-cholesterol-levels-on-sodium-pump-activity-in-subclinical-hypothyroidism
#20
Suparna Roy, Anindya Dasgupta
BACKGROUND: Metabolic dysfunctions characteristic of overt hypothyroidism (OH) start at the early stage of subclinical hypothyroidism (SCH). Na⁺/K⁺-ATPase (the sodium pump) is a transmembrane enzyme that plays a vital role in cellular activities in combination with membrane lipids. We evaluated the effects of early changes in thyroid hormone and membrane cholesterol on sodium pump activity in SCH and OH patients. METHODS: In 32 SCH patients, 35 OH patients, and 34 euthyroid patients, sodium pump activity and cholesterol levels in red blood cell membranes were measured...
February 28, 2017: Endocrinology and Metabolism
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