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Na k atpase

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https://www.readbyqxmd.com/read/29351422/body-mass-specific-na-k-atpase-activity-in-the-medullary-thick-ascending-limb-implications-for-species-dependent-urine-concentrating-mechanisms
#1
Mun Aw, Tamara M Armstrong, C Michele Nawata, Sarah N Bodine, Jeeeun J Oh, Guojun Wei, Kristen K Evans, Mohammad Shahidullah, Timo Rieg, Thomas L Pannabecker
In general, mammalian whole body mass-specific metabolic rate correlates positively with maximal urine concentration (Umax) irrespective of whether or not the species have adapted to arid or mesic habitat. Accordingly, we hypothesized that the thick ascending limb (TAL) of a rodent with markedly higher whole body mass-specific metabolism than rat exhibits a substantially higher TAL metabolic rate as estimated by Na+, K+-ATPase activity and Na+, K+-ATPase α1 gene and protein expression. The kangaroo rat inner stripe of the outer medulla exhibits significantly higher mean Na+, K+-ATPase activity (~70%) compared to 2 rat strains (Sprague-Dawley and Munich-Wistar), extending prior studies showing rat activity exceeds rabbit...
January 3, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/29351414/the-murine-choroid-plexus-epithelium-expresses-the-2cl-h-exchanger-clc-7-and-na-h-exchanger-nhe6-in-the-luminal-membrane-domain
#2
Helle H Damkier, Henriette L Christensen, Inga B Christensen, Qi Wu, Robert A Fenton, Jeppe Praetorius
The choroid plexus epithelium within the brain ventricles secretes the majority of the cerebrospinal fluid (CSF). The luminal Na+,K+-ATPase acts in concert with a host of other transport proteins to mediate efficient fluid secretion across the epithelium. The CSF contains little protein buffer, but the pH value seems nonetheless maintained within narrow limits, even when faced with acid/base challenges. The involvement of choroid plexus acid/base transporters in CSF pH regulation is highlighted by the expression of several acid/base transporters in the epithelium...
December 20, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29351411/disruption-of-the-lens-circulation-causes-calcium-accumulation-and-precipitates-in-connexin-mutant-mice
#3
Junyuan Gao, Peter J Minogue, Eric C Beyer, Richard T Mathias, Viviana M Berthoud
The lens is an avascular organ whose function and survival depend on an internal circulation system. Cx46fs380 mice model a human autosomal dominant cataract caused by a mutant lens connexin. In these mice, fiber cell connexin levels and gap junction coupling are severely decreased. The present studies were conducted to examine components of the lens circulation system that might be altered and contribute to the pathogenesis of cataracts. Lenses from wild type mice and Cx46fs380 heterozygotes and homozygotes were studied at 2 months of age...
January 3, 2018: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29351408/choroid-plexus-epithelial-cells-express-the-adhesion-protein-p-cadherin-at-cell-cell-contacts-and-syntaxin-4-in-the-luminal-membrane-domain
#4
Inga Baasch Christensen, Esben Nees Mogensen, Helle Hasager Damkier, Jeppe Praetorius
The choroid plexus epithelial cells (CPECs) belong to a small group of polarized cells, where the Na+,K+-ATPase is expressed in the luminal membrane. The basic polarity of the cells is, therefore, still debated. We investigated the subcellular distribution of an array of proteins known to play fundamental roles in either in establishing and maintaining basic cell polarity or the polarized delivery and recycling of plasma membrane proteins. Immunofluorescence histochemical analysis was applied to determine the subcellular localization of apical and basolateral membrane determinants...
January 10, 2018: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29350302/antifungal-mechanism-of-sodium-dehydroacetate-against-geotrichum-citri-aurantii
#5
Xu Tang, Qiuli Ouyang, Guoxing Jing, Xingfeng Shao, Nengguo Tao
This study investigated the potential anti-fungal mechanisms of sodium dehydroacetate (SD) against Geotrichum citri-aurantii. The results showed that the cell wall integrity of G. citri-aurantii was not affected, whereas the membrane permeability of G. citri-aurantii mycelia was visibly altered by SD. Dramatic morphological changes of the mycelia, such as loss of cytoplasm, plasmolysis, and dissolution of intracellular substances, were observed by scanning electron microscopy and transmission electron microscopy analyses, indicating that the mycelium is severely damaged by the SD treatment...
January 19, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29347962/primary-explant-culture-and-collagen-i-substrate-enhances-corneal-endothelial-cell-morphology
#6
Judith Zavala, María-Dolores Montalvo-Parra, Guillermo-Isaac Guerrero-Ramírez, Carlos-Alberto Rodríguez-Barrientos, Victor Treviño, Jorge E Valdez-García
OBJECTIVES: Corneal endothelial cell (CEC) isolation and harvest aim to produce engineered grafts to solve donor corneal tissue shortage. To yield high amounts of CEC maintaining morphological and molecular characteristics, several isolation and culture conditions are reported. Here, we combined direct explant culture, with three different coating conditions and a two-step media approach to compare confluence efficiency, morphology, and specific molecular markers expression. DATA DESCRIPTION: Confluence was reached after 2 weeks in the three coating conditions (Matrigel, collagen I, and in uncoated plates) using a two-step approach (proliferative medium without pituitary extract, followed by stabilizer basal medium)...
January 18, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29344767/klotho-attenuates-isoproterenol-induced-hypertrophic-response-in-h9c2-cells-by-activating-na-k-atpase-and-inhibiting-the-reverse-mode-of-na-ca2-exchanger
#7
Gang Tang, Yi Shen, Pan Gao, Shuang-Shuang Song, Liang-Yi Si
Cardiac hypertrophy plays a major role in heart failure and is related to patient morbidity and mortality. Calcium overloading is a main risk for cardiac hypertrophy, and Na+/K+-ATPase (NKA) has been found that it could not only regulate intracellular Na+ levels but also control the intracellular Ca2+ ([Ca2+]i) level through Na+/Ca2+-exchanger (NCX). Recent studies have reported that klotho could affect [Ca2+]i level. In this study, we aimed at exploring the role of klotho in improving isoproterenol-induced hypertrophic response of H9C2 cells...
January 17, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29344503/genetics-of-magnesium-disorders
#8
REVIEW
Heng Li, Shiren Sun, Jianghua Chen, Goushuang Xu, Hanmin Wang, Qi Qian
Background: Magnesium (Mg2+), the second most abundant cation in the cell, is woven into a multitude of cellular functions. Dysmagnesemia is associated with multiple diseases and, when severe, can be life-threatening. Summary: This review discusses Mg2+ homeostasis and function with specific focus on renal Mg2+ handling. Intrarenal channels and transporters related to Mg2+ absorption are discussed. Unraveling the rare genetic diseases with manifestations of dysmagnesemia has greatly increased our understanding of the complex and intricate regulatory network in the kidney, specifically, functions of tight junction proteins including claudin-14, -16, -19, and -10; apical ion channels including: TRPM6, Kv1...
December 2017: Kidney Diseases
https://www.readbyqxmd.com/read/29341798/phenolic-constituents-and-inhibitory-effects-of-hibiscus-sabdariffa-l-sorrel-calyx-on-cholinergic-monoaminergic-and-purinergic-enzyme-activities
#9
Ganiyu Oboh, Taiwo M Adewuni, Adedayo O Ademiluyi, Tosin A Olasehinde, Ayokunle O Ademosun
This study revealed the effect of phenolic extract from Hibiscus sabdariffa L. (sorrel) calyx on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), monoamine oxidase (MAO), and ecto-5' nucleotidase (E-NTDase) activities as well as pro-oxidant-induced oxidative damage in rat brain in vitro. Sorrel extract inhibited AChE (EC50 = 46.96 µg/mL), BChE (EC50 = 40.38 µg/mL), MAO (EC50 = 43.69 µg/mL), and E-NTDase (EC50 = 40.52 µg/mL) and stimulated Na+/K+-ATPase (EC50 = 22.01 µg/mL) activities. The phenolic extract also reduced Fe2+- (EC50 = 22...
January 17, 2018: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/29339801/cardiac-glycoside-aglycones-inhibit-hiv-1-gene-expression-by-a-mechanism-requiring-mek1-2-erk1-2-signaling
#10
Raymond W Wong, Clifford A Lingwood, Mario A Ostrowski, Tyler Cabral, Alan Cochrane
The capacity of HIV-1 to develop resistance to current drugs calls for innovative strategies to control this infection. We aimed at developing novel inhibitors of HIV-1 replication by targeting viral RNA processing-a stage dependent on conserved host processes. We previously reported that digoxin is a potent inhibitor of this stage. Herein, we identify 12 other cardiac glycoside/aglycones or cardiotonic steroids (CSs) that impede HIV growth in HIV-infected T cells from clinical patients at IC50s (1.1-1.3 nM) that are 2-26 times below concentrations used in patients with heart conditions...
January 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29339143/gene-silencing-reveals-multiple-functions-of-na-k-atpase-in-the-salmon-louse-lepeophtheirus-salmonis
#11
Anna Z Komisarczuk, Heidi Kongshaug, Frank Nilsen
Na+/K+-ATPase has a key function in a variety of physiological processes including membrane excitability, osmoregulation, regulation of cell volume, and transport of nutrients. While knowledge about Na+/K+-ATPase function in osmoregulation in crustaceans is extensive, the role of this enzyme in other physiological and developmental processes is scarce. Here, we report characterization, transcriptional distribution and likely functions of the newly identified L. salmonis Na+/K+-ATPase (LsalNa+/K+-ATPase) α subunit in various developmental stages...
January 12, 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29335615/marinobufagenin-is-related-to-elevated-central-and-24-h-systolic-blood-pressures-in-young-black-women-the-african-predict-study
#12
Michél Strauss, Wayne Smith, Wen Wei, Olga V Fedorova, Aletta E Schutte
Marinobufagenin (MBG) is an endogenous steroidal α1-Na+K+-ATPase inhibitor. Because of its role in sodium handling, MBG has been associated with both antihypertensive and prohypertensive effects in normal physiology and pathology. MBG is positively associated with blood pressure in Dahl salt-sensitive rats exhibiting a similar hypertensive phenotype to black populations, characterized by impaired urinary Na+ excretion. However, clinical studies exploring blood pressure (BP)-related effects of MBG in black populations are scant...
January 15, 2018: Hypertension Research: Official Journal of the Japanese Society of Hypertension
https://www.readbyqxmd.com/read/29326243/concentrative-transport-of-antifolates-mediated-by-the-proton-coupled-folate-transporter-pcft-slc46a1-augmentation-by-a-hepes-buffer
#13
Rongbao Zhao, Mitra Najmi, Srinivas Aluri, David C Spray, I David Goldman
The proton-coupled folate transporter (PCFT) is ubiquitously expressed in solid tumors where it delivers antifolates, particularly pemetrexed, into cancer cells. Studies on PCFT-mediated transport, to date, have focused exclusively on the influx of folates and antifolates. This paper addresses the impact of PCFT on concentrative transport, critical to the formation of the active polyglutamate congeners, and at pH levels relevant to the tumor microenvironment. A HeLa-derived cell line was employed in which folate-specific transport was mediated exclusively by PCFT...
January 11, 2018: Molecular Pharmacology
https://www.readbyqxmd.com/read/29321306/digitoxin-suppresses-human-cytomegalovirus-replication-via-na-k-atpase-%C3%AE-1-subunit-dependent-ampk-and-autophagy-activation
#14
Rupkatha Mukhopadhyay, Rajkumar Venkatadri, Jenny Katsnelson, Ravit Arav-Boger
Host-directed therapeutics for human cytomegalovirus (HCMV) requires elucidation of cellular mechanisms that inhibit HCMV. We report on a novel pathway used by cardiac glycosides to inhibit HCMV replication: induction of AMPK activity and autophagy flux through the Na+, K+/ATPase α1 subunit. Our data illustrate an intricate balance between autophagy regulators AMPK, mTOR and ULK1 during infection and treatment with the cardiac glycoside, digitoxin. Both infection and digitoxin induced AMPK phosphorylation, but ULK1 was differentially phosphorylated at unique sites leading to opposing effects on autophagy...
January 10, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29318961/targeting-na-k-atpase-signaling-a-new-approach-to-control-oxidative-stress
#15
Jiang Liu, Megan N Lilly, Joseph I Shapiro
Renal and cardiac function are greatly affected by chronic oxidative stress which can cause many pathophysiological states. The Na/K-ATPase is well-described as an ion pumping enzyme involved in maintaining cellular ion homeostasis; however, in the past two decades, extensive research has been done to understand the signaling function of the Na/K-ATPase and determine its role in physiological and pathophysiological states. Our lab has shown that the Na/K-ATPase signaling cascade can function as an amplifier of reactive oxygen species (ROS) which can be initiated by cardiotonic steroids or increases in ROS...
January 9, 2018: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/29317213/influence-of-strain-origin-on-osmoregulatory-and-endocrine-parameters-of-two-non-native-strains-of-atlantic-salmon-salmo-salar-l
#16
Benoît Bernard, Kevin Chantung Sobandi, Veerle Darras, Xavier Rollin, Syaghalirwa N M Mandiki, Patrick Kestemont
Non-native strains of Atlantic salmon are used in reinstatement trials where populations are extinct. Environmental cues like photoperiod and temperature are known to influence the smolting process and there is evidence of strain-, stock- or population-specific differences associated with seaward migration or smoltification. The objective of this study was to compare morphological, osmoregulatory and endorcrine features between two strains, one originating from a cold and short river in Ireland (Cong) and another from a long and warm river in France (Loire-Allier), reared under Belgian conditions in order to highlight major differences in restocking adaptability...
January 6, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29315599/influence-of-the-drying-method-on-the-bioactive-compounds-and-pharmacological-activities-of-rhubarb
#17
Jiachen Sun, Yueting Wu, Shengjie Dong, Xia Li, Wenyuan Gao
BACKGROUND: Raw rhubarb samples that have been subjected to different drying procedures will have different therapeutic effects possibly due to processing-induced variations in the chemical composition. In the present work, the fresh materials were processed by smoking, sun-drying, shade-drying and oven-drying at low, moderate and high temperatures. To facilitate the selection of a suitable drying method for rhubarb, the quality of rhubarb processed under various drying conditions was evaluated based on the simultaneous determination of multiple bioactive constituents in combination with bioactivity assays...
January 9, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29313547/the-p-type-atpase-inhibiting-potential-of-polyoxotungstates
#18
Nadiia Gumerova, Lukáš Krivosudský, Gil Fraqueza, Joscha Breibeck, Emir Al-Sayed, Elias Tanuhadi, Aleksandar Bijelic, Juan Fuentes, Manuel Aureliano, Annette Rompel
Polyoxometalates (POMs) are transition metal complexes that exhibit a broad diversity of structures and properties rendering them promising for biological purposes. POMs are able to inhibit a series of biologically important enzymes, including phosphatases, and thus are able to affect many biochemical processes. In the present study, we analyzed and compared the inhibitory effects of nine different polyoxotungstates (POTs) on two P-type ATPases, Ca2+-ATPase from skeletal muscle and Na+/K+-ATPase from basal membrane of skin epithelia...
January 9, 2018: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/29313218/thallium-induced-toxicity-in-rat-brain-crude-synaptosomal-mitochondrial-fractions-is-sensitive-to-anti-excitatory-and-antioxidant-agents
#19
Marisol Maya-López, María Verónica Mireles-García, Monserrat Ramírez-Toledo, Ana Laura Colín-González, Sonia Galván-Arzate, Isaac Túnez, Abel Santamaría
The mechanisms by which the heavy metal thallium (Tl+) produces toxicity in the brain remain unclear. Herein, isolated synaptosomal/mitochondrial P2 crude fractions from adult rat brains were exposed to Tl+ (5-250 μM) for 30 min. Three toxic endpoints were evaluated: mitochondrial dysfunction, lipid peroxidation, and Na+/K+-ATPase activity inhibition. Concentration-response curves for two of these endpoints revealed the optimum concentration of Tl+ to induce damage in this preparation, 5 μM. Toxic markers were also estimated in preconditioned synaptosomes incubated in the presence of the N-methyl-D-aspartate receptor antagonist kynurenic acid (KYNA, 50 μM), the cannabinoid receptor agonist WIN 55,212-2 (1 μM), or the antioxidant S-allyl-L-cysteine (SAC, 100 μM)...
January 8, 2018: Neurotoxicity Research
https://www.readbyqxmd.com/read/29310813/effect-of-sevoflurane-on-the-atpase-activity-of-hippocampal-neurons-in-a-rat-model-of-cerebral-ischemia-reperfusion-injury-via-the-camp-pka-signaling-pathway
#20
Tie-Jun Liu, Jin-Cun Zhang, Xiao-Zeng Gao, Zhi-Bin Tan, Jian-Jun Wang, Pan-Pan Zhang, Ai-Bin Cheng, Shu-Bo Zhang
We aim to investigate the effects of sevoflurane on the ATPase activity of the hippocampal neurons in rats with cerebral ischemia-reperfusion injury (IRI) via the cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) signaling pathway. Sixty rats were assigned into the normal, model and sevoflurane groups (n = 20, the latter two groups were established as focal cerebral IRI models). The ATPase activity was detected using an ultramicro Na (+)-K (+)-ATP enzyme kit. Immunohistochemical staining was used to detect the positive protein expression of cAMP and PKA...
January 2018: Kaohsiung Journal of Medical Sciences
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