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https://www.readbyqxmd.com/read/28822161/-synergistic-mechanism-of-traditional-chinese-medicine-based-on-target-combination-of-pept1-and-ppar%C3%AE
#1
Lian-Sheng Qiao, Yan-Kun Chen, Gang-Gang Luo, Fang Lu, Si-Jia Liu, Gong-Yu Li, Yan-Ling Zhang
Synergistic effect is main pharmacological mechanism of traditional Chinese medicine(TCM). The research method based on the key targets combination is an important method to explore the synergistic effect of TCM. Peptide transporter 1 (PepT1) is an essential target for drug uptake into the bloodstream, accounting for about 50% of the total transporter protein content from the small intestine. Peroxisome proliferator-activated receptor α(PPARα) is the lipid-lowering target of fibrates, which have a good hypolipidemic effect by activating PPARα...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822131/metabolic-engineering-of-photorespiration
#2
Martin K M Engqvist, Veronica G Maurino
The introduction of two alternative glycolate catabolic pathways in the chloroplasts of Arabidopsis thaliana rendered plants with increased biomass. To introduce these synthetic pathways, the selected genes were stepwise integrated in the nuclear genome of wild-type plants. These plants were transformed by Agrobacterium tumefaciens carrying the binary vectors using the floral dip method. Selection of transformants was conducted using different selection agents and the expression of the transgenes was confirmed by PCR and enzyme activity measurements...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28821960/comparative-analysis-of-germ-cells-and-dna-of-the-genus-amblyomma-adding-new-data-on-amblyomma-maculatum-and-amblyomma-ovale-species-acari-ixodidae
#3
Fredy Arvey Rivera-Páez, Bruno Rodrigues Sampieri, Marcelo Bahia Labruna, Renata da Silva Matos, Thiago Fernandes Martins, Maria Izabel Camargo-Mathias
Among tick species, members of the subfamily Amblyomminae have received special attention, since they serve as vectors for pathogens such as Rickettsia spp. and display cryptic species complexes that make their taxonomical classification challenging. Amblyomma ovale, Amblyomma maculatum, and other species of the genus Amblyomma have shown a long history of taxonomic controversies. Spermiotaxonomy has proved to be a valuable tool in the solution of systematic conflicts in Metazoa that can aid molecular and external morphological analyses in ticks and, overall, provide more robust analyses and results...
August 18, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28821927/control-of-wrist-movement-in-deafferented-man-evidence-for-a-mixed-strategy-of-position-and-amplitude-control
#4
R Chris Miall, P Haggard, J D Cole
There is a continuing debate about control of voluntary movement, with conflicted evidence about the balance between control of movement vectors (amplitude control) that implies knowledge of the starting position for accuracy, and equilibrium point or final position control, that is independent of the starting conditions. We tested wrist flexion and extension movements in a man with a chronic peripheral neuronopathy that deprived him of proprioceptive knowledge of his wrist angles. In a series of experiments, we demonstrate that he could scale the amplitude of his wrist movements in flexion/extension, even without visual feedback, and appeared to adopt a strategy of moving via a central wrist position when asked to reach target angles from unknown start locations...
August 18, 2017: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
https://www.readbyqxmd.com/read/28821784/high-copy-and-stable-expression-of-the-xylanase-xynhb-in-saccharomyces-cerevisiae-by-rdna-mediated-integration
#5
Cheng Fang, Qinhong Wang, Jonathan Nimal Selvaraj, Yuling Zhou, Lixin Ma, Guimin Zhang, Yanhe Ma
Xylanase is a widely-used additive in baking industry for enhancing dough and bread quality. Several xylanases used in baking industry were expressed in different systems, but their expression in antibiotic free vector system is highly essential and safe. In the present study, an alternative rDNA-mediated technology was developed to increase the copy number of target gene by integrating it into Saccharomyces cerevisiae genome. A xylanase-encoding gene xynHB from Bacillus sp. was cloned into pHBM367H and integrated into S...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821712/emergence-of-recombinant-mayaro-virus-strains-from-the-amazon-basin
#6
Carla Mavian, Brittany D Rife, James Jarad Dollar, Eleonora Cella, Massimo Ciccozzi, Mattia C F Prosperi, John Lednicky, J Glenn Morris, Ilaria Capua, Marco Salemi
Mayaro virus (MAYV), causative agent of Mayaro Fever, is an arbovirus transmitted by Haemagogus mosquitoes. Despite recent attention due to the identification of several cases in South and Central America and the Caribbean, limited information on MAYV evolution and epidemiology exists and represents a barrier to prevention of further spread. We present a thorough spatiotemporal evolutionary study of MAYV full-genome sequences collected over the last sixty years within South America and Haiti, revealing recent recombination events and adaptation to a broad host and vector range, including Aedes mosquito species...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821668/sensory-biased-and-multiple-demand-processing-in-human-lateral-frontal-cortex
#7
Abigail L Noyce, Nishmar Cestero, Samantha W Michalka, Barbara G Shinn-Cunningham, David C Somers
The functionality of much of human lateral frontal cortex (LFC) has been characterized as 'multiple demand' as these regions appear to support a broad range of cognitive tasks. In contrast to this domain-general account, recent evidence indicates that portions of LFC are consistently selective for sensory modality. Michalka et al. (2015) reported two bilateral regions that are biased for visual attention, superior precentral sulcus (sPCS) and inferior precentral sulcus (iPCS), interleaved with two bilateral regions that are biased for auditory attention, transverse gyrus intersecting precentral sulcus (tgPCS) and caudal inferior frontal sulcus (cIFS)...
August 8, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28820881/vector-competence-of-populations-of-aedes-aegypti-from-three-distinct-cities-in-kenya-for-chikungunya-virus
#8
Sheila B Agha, Edith Chepkorir, Francis Mulwa, Caroline Tigoi, Samwel Arum, Milehna M Guarido, Peris Ambala, Betty Chelangat, Joel Lutomiah, David P Tchouassi, Michael J Turell, Rosemary Sang
BACKGROUND: In April, 2004, chikungunya virus (CHIKV) re-emerged in Kenya and eventually spread to the islands in the Indian Ocean basin, South-East Asia, and the Americas. The virus, which is often associated with high levels of viremia in humans, is mostly transmitted by the urban vector, Aedes aegypti. The expansion of CHIKV presents a public health challenge both locally and internationally. In this study, we investigated the ability of Ae. aegypti mosquitoes from three distinct cities in Kenya; Mombasa (outbreak prone), Kisumu, and Nairobi (no documented outbreak) to transmit CHIKV...
August 18, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28820740/subspace-based-interference-removal-methods-for-a-multichannel-biomagnetic-sensor-array
#9
Kensuke Sekihara, Srikantan S Nagarajan
OBJECTIVE: In biomagnetic signal processing, the theory of the signal subspace has been applied to removing interfering magnetic fields, and a representative algorithm is the signal space projection algorithm, in which the signal/interference subspace is defined in the spatial domain as the span of signal/interference-source lead field vectors. This paper extends the notion of this conventional (spatial domain) signal subspace by introducing a new definition of signal subspace in the time domain...
August 18, 2017: Journal of Neural Engineering
https://www.readbyqxmd.com/read/28820722/house-structure-is-associated-with-plasmodium-falciparum-infection-in-a-low-transmission-setting-in-southern-zambia
#10
Matthew M Ippolito, Kelly M Searle, Harry Hamapumbu, Timothy M Shields, Jennifer C Stevenson, Philip E Thuma, William J Moss, For The Southern Africa International Center Of Excellence For Malaria Research
House structure may influence the risk of malaria by affecting mosquito entry and indoor resting. Identification of construction features associated with protective benefits could inform vector control approaches, even in low-transmission settings. We examined the association between house structure and malaria prevalence in a cross-sectional analysis of 2,788 children and adults residing in 866 houses in a low-transmission area of Southern Province, Zambia, over the period 2008-2012. Houses were categorized according to wall (brick/cement block or mud/grass) and roof (metal or grass) material...
August 7, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28820719/central-nervous-system-infection-associated-with-orientia-tsutsugamushi-in-south-korea
#11
Han Sang Lee, Jun-Sang Sunwoo, Seon-Jae Ahn, Jangsup Moon, Jung-Ah Lim, Jin-Sun Jun, Woo-Jin Lee, Soon-Tae Lee, Keun-Hwa Jung, Kyung-Il Park, Ki-Young Jung, Sang Kun Lee, Kon Chu
Orientia tsutsugamushi is a major cause of vector-borne infection in Asia. Prompt recognition and appropriate treatment are crucial because of its potentially fatal complications and lack of response to beta-lactam antibiotics. The present study retrospectively evaluated the clinical characteristics and laboratory findings of 16 patients with scrub typhus-related central nervous system (CNS) infections. Single titers ≥ 1:40 of total serum antibodies against O. tsutsugamushi detected by an indirect immunofluorescent assay were considered as positive results...
July 31, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28820716/evaluation-of-community-directed-operation-of-black-fly-traps-for-entomological-surveillance-of-onchocerca-volvulus-transmission-in-the-madi-mid-north-focus-of-onchocerciasis-in-northern-uganda
#12
Denis Loum, Charles R Katholi, Thomson Lakwo, Peace Habomugisha, Edridah M Tukahebwa, Thomas R Unnasch
Entomological measures of transmission are important metrics specified by the World Health Organization to document the suppression and interruption of transmission of Onchocerca volvulus, the causative agent of onchocerciasis. These metrics require testing of large numbers of vector black flies. Black fly collection has relied on human landing collections, which are inefficient and potentially hazardous. As the focus of the international community has shifted from onchocerciasis control to elimination, replacement of human landing collections has become a priority...
July 31, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28820715/limited-transmission-potential-of-takeda-s-tetravalent-dengue-vaccine-candidate-by-aedes-albopictus
#13
Elizabeth A Dietrich, Yee Tsuey Ong, Janae L Stovall, Hansi Dean, Claire Y-H Huang
Recombinant live-attenuated chimeric tetravalent dengue vaccine viruses, TDV-1, -2, -3, and -4, contain the premembrane and envelope genes of dengue virus serotypes 1-4 in the replicative background of the attenuated dengue virus type-2 (DENV-2) PDK-53 vaccine strain. Previous results have shown that these recombinant vaccine viruses demonstrate limited infection and dissemination in Aedes aegypti and are unlikely to be transmitted by the primary mosquito vector of DENVs. In this report, we expand this analysis by assessing vector competence of all four serotypes of the TDV virus in Aedes albopictus, the secondary mosquito vector of DENVs...
August 14, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28820695/prevalence-and-seroprevalence-of-trypanosoma-cruzi-infection-in-a-military-population-in-texas
#14
Bryant J Webber, Mary T Pawlak, Sandra Valtier, Candelaria C Daniels, Charla C Tully, Edward J Wozniak, Walter D Roachell, Francisco X Sanchez, Audra A Blasi, Thomas L Cropper
Recent biosurveillance findings at Joint Base San Antonio (JBSA), a large military installation located in south-central Texas, indicate the potential for vector-borne human Chagas disease. A cross-sectional study was conducted to determine the prevalence and seroprevalence of Trypanosoma cruzi infection in highest risk subpopulations on the installation, including students and instructors who work and sleep in triatomine-endemic field settings. Real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and indirect immunofluorescent antibody assay were performed on enrolled subjects (N = 1,033), none of whom tested positive for T...
August 14, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28820690/scaling-up-of-an-innovative-intervention-to-reduce-risk-of-dengue-chikungunya-and-zika-transmission-in-uruguay-in-the-framework-of-an-intersectoral-approach-with-and-without-community-participation
#15
César Basso, Elsa García da Rosa, Rosario Lairihoy, Ruben M Caffera, Ingrid Roche, Cristina González, Ricardo da Rosa, Alexis Gularte, Eduardo Alfonso-Sierra, Max Petzold, Axel Kroeger, Johannes Sommerfeld
To contribute to the prevention of dengue, chikungunya, and Zika, a process of scaling up an innovative intervention to reduce Aedes aegypti habitats, was carried out in the city of Salto (Uruguay) based on a transdisciplinary analysis of the eco-bio-social determinants. The intervention in one-third of the city included the distributions of plastic bags for all households to collect all discarded water containers that were recollected by the Ministry of Health and the Municipality vector control services. The results were evaluated in 20 randomly assigned clusters of 100 households each, in the intervention and control arm...
August 7, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28820490/the-potentiodynamic-bottom-up-growth-of-the-tin-oxide-nanostructured-layer-for-gas-analytical-multisensor-array-chips
#16
Fedor S Fedorov, Dmitry Podgainov, Alexey Varezhnikov, Andrey Lashkov, Michail Gorshenkov, Igor Burmistrov, Martin Sommer, Victor Sysoev
We report a deposition of the tin oxide/hydroxide nanostructured layer by the potentiodynamic method from acidic nitrate solutions directly over the substrate, equipped with multiple strip electrodes which is employed as a gas-analytical multisensor array chip. The electrochemical synthesis is set to favor the growth of the tin oxide/hydroxide phase, while the appearance of metallic Sn is suppressed by cycling. The as-synthesized tin oxide/hydroxide layer is characterized by mesoporous morphology with grains, 250-300 nm diameter, which are further crystallized into fine SnO₂ poly-nanocrystals following heating to 300 °C for 24 h just on the chip...
August 18, 2017: Sensors
https://www.readbyqxmd.com/read/28820478/detection-of-interactions-between-proteins-by-using-legendre-moments-descriptor-to-extract-discriminatory-information-embedded-in-pssm
#17
Yan-Bin Wang, Zhu-Hong You, Li-Ping Li, Yu-An Huang, Hai-Cheng Yi
Protein-protein interactions (PPIs) play a very large part in most cellular processes. Although a great deal of research has been devoted to detecting PPIs through high-throughput technologies, these methods are clearly expensive and cumbersome. Compared with the traditional experimental methods, computational methods have attracted much attention because of their good performance in detecting PPIs. In our work, a novel computational method named as PCVM-LM is proposed which combines the probabilistic classification vector machine (PCVM) model and Legendre moments (LMs) to predict PPIs from amino acid sequences...
August 18, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28820323/non-surgical-model-for-alveolar-bone-regeneration-by-bone-morphogenetic-protein-2-7-gene-therapy
#18
Mariko Kawai, Yo-Hei Kataoka, Junya Sonobe, Hiromitsu Yamamoto, Masakazu Inubushi, Takuya Ishimoto, Takayoshi Nakano, Hiroki Maruyama, Jun-Ichi Miyazaki, Toshio Yamamoto, Kazuhisa Bessho, Kiyoshi Ohura
BACKGROUND: Alveolar bone is a critical tissue for tooth retention; however, once alveolar bone is lost, it may not spontaneously regenerate. Currently, bone grafts or artificial bone are commonly used for alveolar bone regeneration therapy. However, these therapies require surgical procedures, which present risks, particularly in aged patients. Therefore, the development of alveolar bone regeneration techniques that do not require surgical procedures is critical. It is well known that stem cells present in the periosteal and periodontal ligament may be induced to differentiate into osteogenic cells...
August 18, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28820183/technique-of-retinal-gene-therapy-delivery-of-viral-vector-into-the-subretinal-space
#19
K Xue, M Groppe, A P Salvetti, R E MacLaren
PurposeSafe and reproducible delivery of gene therapy vector into the subretinal space is essential for successful targeting of the retinal pigment epithelium (RPE) and photoreceptors. The success of surgery is critical for the clinical efficacy of retinal gene therapy. Iatrogenic detachment of the degenerate (often adherent) retina in patients with hereditary retinal degenerations and small volume (eg, 0.1 ml) subretinal injections pose new surgical challenges.MethodsOur subretinal gene therapy technique involved pre-operative planning with optical coherence tomography (OCT) and autofluorescence (AF) imaging, 23 G pars plana vitrectomy, internal limiting membrane staining with Membrane Blue Dual (DORC BV, Zuidland, Netherlands), a two-step subretinal injection using a 41 G Teflon tipped cannula (DORC) first with normal saline to create a parafoveal bleb followed by slow infusion of viral vector via the same self-sealing retinotomy...
August 18, 2017: Eye
https://www.readbyqxmd.com/read/28820179/effects-of-dck-knockdown-on-proliferation-apoptosis-and-tumorigenicity-in-vivo-of-cervical-cancer-hela-cells
#20
Q-Y Shang, C-S Wu, H-R Gao
The present study explored the effect that deoxycytidine kinase (DCK) knockdown had on proliferation, apoptosis and tumorigenicity in vivo of cervical cancer HeLa cells. Human cervical cancer HeLa cells that had received no prior treatment were selected from the HeLa group. The HeLa-negative control (NC) group consisted of cells that had undergone an empty vector treatment, and finally the HeLa-short hairpin RNA (shRNA) group included cells that were treated by means of shRNA-DCK expression. DCK expressions were evaluated by quantitative real-time polymerase chain reaction in addition to western blotting assays...
August 18, 2017: Cancer Gene Therapy
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