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https://www.readbyqxmd.com/read/29232693/host-factors-that-promote-retrotransposon-integration-are-similar-in-distantly-related-eukaryotes
#1
Sudhir Kumar Rai, Maya Sangesland, Michael Lee, Caroline Esnault, Yujin Cui, Atreyi Ghatak Chatterjee, Henry L Levin
Retroviruses and Long Terminal Repeat (LTR)-retrotransposons have distinct patterns of integration sites. The oncogenic potential of retrovirus-based vectors used in gene therapy is dependent on the selection of integration sites associated with promoters. The LTR-retrotransposon Tf1 of Schizosaccharomyces pombe is studied as a model for oncogenic retroviruses because it integrates into the promoters of stress response genes. Although integrases (INs) encoded by retroviruses and LTR-retrotransposons are responsible for catalyzing the insertion of cDNA into the host genome, it is thought that distinct host factors are required for the efficiency and specificity of integration...
December 12, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/29232506/thiolactone-chemistry-based-combinatorial-methodology-to-construct-multifunctional-polymers-for-efficacious-gene-delivery
#2
Zengshi Zha, Yongyi Hu, Jean Mukerabigwi, Weijian Chen, Yuheng Wang, Chuanxin He, Zhishen Ge
Hydrophobic segments and amino moieties in polymeric nonviral gene vectors play important roles in overcoming a cas-cade of barriers for efficient gene delivery. However, it remains a great challenge to facilely construct well-defined multi-functional polymers through optimization of the amino and hydrophobic groups. Herein, we choose thiolactone chemistry to perform the ring opening reaction of varying hydrophobic groups-modified thiolactones by various amines to generate mercapto groups for further Michael addition reaction with poly[2-(acryloyloxy)ethyl methacrylate] (PAOEMA)...
December 12, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29232453/detection-of-zika-virus-in-aedes-mosquitoes-from-mexico
#3
Herón Huerta, Jesús Felipe González-Roldán, Gustavo Sánchez-Tejeda, Fabián Correa-Morales, Francisco Eduardo Romero-Contreras, Raúl Cárdenas-Flores, Mónica Liliana Rangel-Martínez, Juan Manuel Mata-Rivera, José de Jesús Siller-Martínez, Gonzalo M Vazquez-Prokopec, Pablo Manrique-Saide, Felipe Dzul-Manzanilla, Mauricio Vázquez-Pichardo, Claudia Rosales-Jiménez, María de la Luz Torres-Rodríguez, Alma Núñez-León, Belem Torres-Longoria, Irma López-Martínez, Cuitláhuac Ruíz-Matus, Pablo Antonio Kuri-Morales, José Alberto Díaz-Quiñónez
Background: We report on the results of an entomovirological surveillance system of Aedes populations performed by the Ministry of Health of the central state of San Luis Potosí, Mexico. Methods: Indoor adult Aedes aegypti and Aedes albopictus pools collected at San Martín, Tamazunchale, Ciudad Valles, Metlapa, Ebano, Tamuin and Axtla during the dry season of 2016 were examined for the presence of dengue (DENV), chikungunya (CHIKV) and Zika (ZIKV) viruses using real-time PCR...
July 1, 2017: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29232402/chagas-disease-vector-blood-meal-sources-identified-by-protein-mass-spectrometry
#4
Judith I Keller, Bryan A Ballif, Riley M St Clair, James J Vincent, M Carlota Monroy, Lori Stevens
Chagas disease is a complex vector borne parasitic disease involving blood feeding Triatominae (Hemiptera: Reduviidae) insects, also known as kissing bugs, and the vertebrates they feed on. This disease has tremendous impacts on millions of people and is a global health problem. The etiological agent of Chagas disease, Trypanosoma cruzi (Kinetoplastea: Trypanosomatida: Trypanosomatidae), is deposited on the mammalian host in the insect's feces during a blood meal, and enters the host's blood stream through mucous membranes or a break in the skin...
2017: PloS One
https://www.readbyqxmd.com/read/29232397/distribution-and-prevalence-of-vector-borne-diseases-in-california-chipmunks-tamias-spp
#5
Mary H Straub, Austin N Roy, Amanda Martin, Kathleen E Sholty, Nicole Stephenson, Janet E Foley
California, with 13 chipmunk (Tamias) species, has more than any other state or country, occupying habitats ranging from chaparral to the high peaks of the Sierra Nevada. Chipmunks host zoonotic pathogens including Yersinia pestis, Anaplasma phagocytophilum, relapsing fever (RF) Borrelia spp., Borrelia burgdorferi, and spotted fever group (SFG) Rickettsia species. Chipmunk species are often not differentiated by public health workers, yet different species utilize different ecological niches and may have intrinsically different capacities for maintaining vector-borne pathogens and infecting vectors...
2017: PloS One
https://www.readbyqxmd.com/read/29232388/more-than-the-eyes-can-see-the-worrying-scenario-of-canine-leishmaniasis-in-the-brazilian-side-of-the-triple-border
#6
Vanete Thomaz Soccol, Aline Kuhn Sbruzzi Pasquali, Eliane Maria Pozzolo, André de Souza Leandro, Luciana Chiyo, Rafael Antunes Baggio, Mario Sergio Michaliszyn, Carlos Silva, Patrícia Hoerner Cubas, Ricardo Peterlle, Otacilio Lopes de Souza Paz, Ivana Lucia Belmonte, Alceu Bisetto-Junior
A cross-sectional epidemiological study in the extreme-west of the state of Paraná was carried out to access the prevalence, distribution, and risk variables of canine Visceral Leishmaniases (cVL). This study was conducted in three areas, two cities of far west of Parana state: Foz do Iguaçu (FI) and Santa Terezinha de Itaipu (STI), and along two transects between these two municipalities. To sample the entire urban area, the cities (FI and STI) were divided into a grid of squares of 400 m2 (patch). Among the 526 patches, 123 in FI, 40 in the transects and 33 in STI were selected according to the 'worst scenario' criterion...
2017: PloS One
https://www.readbyqxmd.com/read/29232385/sustaining-visceral-leishmaniasis-elimination-in-bangladesh-could-a-policy-brief-help
#7
Alyssa Fitzpatrick, Noor Saad M S Al-Kobaisi, Jessica Beitman Maya, Yu Ren Chung, Satyender Duhan, Erdene Elbegdorj, Sushant Jain, Edward Kuhn, Alexandra Nastase, Be-Nazir Ahmed, Piero Olliaro
Bangladesh has made significant progress towards elimination of visceral leishmaniasis, and is on track to achieve its target of less than one case per 10,000 inhabitants in each subdistrict in 2017. As the incidence of disease falls, it is likely that the political capital and financial resources dedicated towards the elimination of visceral leishmaniasis may decrease, raising the prospect of disease resurgence. Policy memos may play a crucial role during the transition of the elimination plan from the 'attack' to the 'consolidation' and 'maintenance' phases, highlighting key stakeholders and areas where ongoing investment is crucial...
December 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29232111/a-hand-held-optoelectronic-nose-for-the-identification-of-liquors
#8
Zheng Li, Kenneth S Suslick
Successful discrimination of 14 representative liquors (including scotch, bourbon and rye whiskies, brandy, and vodka) was achieved using a 36-element colorimetric sensor array comprising multiple classes of cross-reactive, chemically responsive inks. In combination with a palm-sized image analyzer, the sensor array permits real-time identification of liquor products based on vapor analysis within 2 min. Changes in sensor spot colors before and after exposure to the vapors of the liquors that are partially oxidized as they are pumped over the sensor array provides a unique color difference pattern for each analyte...
December 12, 2017: ACS Sensors
https://www.readbyqxmd.com/read/29231864/vibration-sensor-monitoring-of-nickel-titanium-alloy-turning-for-machinability-evaluation
#9
Tiziana Segreto, Alessandra Caggiano, Sara Karam, Roberto Teti
Nickel-Titanium (Ni-Ti) alloys are very difficult-to-machine materials causing notable manufacturing problems due to their unique mechanical properties, including superelasticity, high ductility, and severe strain-hardening. In this framework, the aim of this paper is to assess the machinability of Ni-Ti alloys with reference to turning processes in order to realize a reliable and robust in-process identification of machinability conditions. An on-line sensor monitoring procedure based on the acquisition of vibration signals was implemented during the experimental turning tests...
December 12, 2017: Sensors
https://www.readbyqxmd.com/read/29231282/new-insight-into-the-synthesis-and-biological-activity-of-the-polymeric-materials-consisting-of-folic-acid-and-%C3%AE-cyclodextrin
#10
Artur Kasprzak, Ireneusz P Grudzinski, Magdalena Bamburowicz-Klimkowska, Andrzej Parzonko, Maciej Gawlak, Magdalena Poplawska
This work presents a very new look at folate targeting and is focused on synthesizing and assessing the biological activity of folic acid-targeted drug delivery materials based on β-cyclodextrin. Both folic acid and β-cyclodextrin have been covalently conjugated to branched polyethylenimine as the polymeric vector. Host-guest inclusion of folic acid into a β-cyclodextrin cavity, demonstrated by means of the spectroscopic methods (2-D NMR, IR, UV-Vis), is found to be of crucial importance for biological activity of nanotherapeutics...
December 12, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29231227/viscoelastic-behaviour-and-relaxation-modes-of-one-polyamic-acid-organogel-studied-by-rheometers-and-dynamic-light-scattering
#11
Ensong Zhang, Yong Zhao, Wenke Yang, Hongxiang Chen, Wei Liu, Xuemin Dai, Xuepeng Qiu, Xiangling Ji
A novel polyamic acid (PAA from BAPMPO-BPDA) organogel was synthesized and characterized via dynamic light scattering (DLS), a classical rheometer, and diffusion wave spectroscopy (DWS). In situ monitoring was performed using a classical rheometer to observe the formation of the PAA organogel. The rheological curves confirm the formation of the PAA gel network and the origin of hydrogen bonding from the -NH- group (donor) and P[double bond, length as m-dash]O group (acceptor). The autocorrelation functions of PAA under different conditions (pure gel, gel with NaNO3, gel with formamide) are measured via DLS, and different characteristic times are obtained via the CONTIN method...
December 12, 2017: Soft Matter
https://www.readbyqxmd.com/read/29230823/decreasing-fructose-1-6-bisphosphate-aldolase-activity-reduces-plant-growth-and-tolerance-to-chilling-stress-in-tomato-seedlings
#12
Bingbing Cai, Qiang Li, Fengjiao Liu, Huangai Bi, Xizhen Ai
In northern China, low temperatures are the most common abiotic stresses for tomato plants cultivated in solar-greenhouse in winter. We recently found that the expression and enzyme activity of fructose 1,6-bisphosphate aldolases in tomato (FBAs), which are important enzymes in the Calvin-Benson cycle (CBC), were significantly altered in tomato seedlings subjected to heat/cold stresses. In order to study the role of FBA in photosynthesis and in regulating cold stress responses of tomato seedlings (Solanum lycopersicum), we transformed a tomato inbred line (FF) with RNA interference (RNAi) vector containing SlFBA7 reverse tandem repeat sequence...
December 12, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/29230570/the-relationship-between-systolic-vector-flow-mapping-parameters-and-left-ventricular-cardiac-function-in-healthy-dogs
#13
Seijirow Goya, Tomoki Wada, Kazumi Shimada, Daiki Hirao, Ryou Tanaka
Vector flow mapping (VFM) is a novel echocardiographic technology that shows blood flow vectors and vortexes, enabled the hydrokinetic evaluation of hemodynamics within the left ventricle. VFM provides several unique parameters: circulation, vorticity, vortex area, and energy loss. The present study aims to reveal a relationship between VFM parameters and cardiac function. Five healthy Beagle dogs were anesthetized and administered with dobutamine (0, 2, 4, 8, 12 µg/kg/min). Pressure-volume diagrams were acquired to assess cardiac function using pressure-volume conductance catheter...
December 11, 2017: Heart and Vessels
https://www.readbyqxmd.com/read/29230236/post-transcriptional-regulation-of-pcsk9-by-mir-191-mir-222-and-mir-224
#14
Parisa Naeli, Fatemeh Mirzadeh Azad, Mahshid Malakootian, Nabil G Seidah, Seyed J Mowla
Since proprotein convertase subtilisin kexin 9 (PCSK9) discovery, a gene involved in LDL metabolism regulation and cardiovascular diseases (CVD), many therapeutic strategies have been introduced for direct targeting of PCSK9. The main goal of these strategies has been to reduce PCSK9 protein level either by application of antibodies or inhibition of its production. In this study, we have tried to discover microRNAs (miRNAs) which can target, and hence regulate, PCSK9 expression. Using bioinformatics tools, we selected three microRNAs with binding sites on 3'-UTR of PCSK9...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/29230228/overexpression-of-smmyc2-increases-the-production-of-phenolic-acids-in-salvia-miltiorrhiza
#15
Na Yang, Wenping Zhou, Jiao Su, Xiaofan Wang, Lin Li, Liru Wang, Xiaoyan Cao, Zhezhi Wang
MYC2 is a core transcription factor in the plant response to jasmonates. It also functions in secondary metabolism and various processes for growth and development. However, the knowledge about its role in Salvia miltiorrhiza is still very limited. We determined that the biosynthesis of salvianolic acid B (Sal B) was strongly induced in 2-month-old transgenic plants that over-expressed SmMYC2. In the roots of transgenic line 12 that over-expressed SmMYC2 (OEM-12), the Sal B concentration was as high as 5.95 ± 0...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29230202/development-of-a-novel-escherichia-coli-kocuria-shuttle-vector-using-the-cryptic-pkpal3-plasmid-from-k-palustris-ipufs-1-and-its-utilization-in-producing-enantiopure-s-styrene-oxide
#16
Hiroshi Toda, Nobuya Itoh
The novel cryptic pKPAL3 plasmid was isolated from the Gram-positive microorganism Kocuria palustris IPUFS-1 and characterized in detail. pKPAL3 is a circular plasmid that is 4,443 bp in length. Open reading frame (ORF) and homology search analyses indicated that pKPAL3 possesses four ORFs; however, there were no replication protein coding genes predicted in the plasmid. Instead, there were two nucleotide sequence regions that showed significant identities with untranslated regions of K. rhizophila DC2201 (NBRC 103217) genomic sequences, and these sequences were essential for autonomous replication of pKPAL3 in Kocuria cells...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29229947/recombinant-human-osteopontin-expressed-in-nicotiana-benthamiana-stimulates-osteogenesis-related-genes-in-human-periodontal-ligament-cells
#17
Kaewta Rattanapisit, Supaniga Abdulheem, Daneeya Chaikeawkaew, Anchanee Kubera, Hugh S Mason, Julian K-C Ma, Prasit Pavasant, Waranyoo Phoolcharoen
Tissue engineering aims to utilise biologic mediators to facilitate tissue regeneration. Several recombinant proteins have potential to mediate induction of bone production, however, the high production cost of mammalian cell expression impedes patient access to such treatments. The aim of this study is to produce recombinant human osteopontin (hOPN) in plants for inducing dental bone regeneration. The expression host was Nicotiana benthamiana using a geminiviral vector for transient expression. OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni affinity chromatography...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29229938/exploiting-anopheles-responses-to-thermal-odour-and-visual-stimuli-to-improve-surveillance-and-control-of-malaria
#18
Frances M Hawkes, Roch K Dabiré, Simon P Sawadogo, Stephen J Torr, Gabriella Gibson
Mosquito surveillance and control are at the heart of efforts to eliminate malaria, however, there remain significant gaps in our understanding of mosquito behaviour that impede innovation. We hypothesised that a combination of human-associated stimuli could be used to attract and kill malaria vectors more successfully than individual stimuli, and at least as well as a real human. To test this in the field, we quantified Anopheles responses to olfactory, visual and thermal stimuli in Burkina Faso using a simple adhesive trap...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29229829/psma-targeted-polyinosine-polycytosine-vector-induces-prostate-tumor-regression-and-invokes-an-antitumor-immune-response-in-mice
#19
Yael Langut, Alaa Talhami, Samarasimhareddy Mamidi, Alexei Shir, Maya Zigler, Salim Joubran, Anna Sagalov, Efrat Flashner-Abramson, Nufar Edinger, Shoshana Klein, Alexander Levitzki
There is an urgent need for an effective treatment for metastatic prostate cancer (PC). Prostate tumors invariably overexpress prostate surface membrane antigen (PSMA). We designed a nonviral vector, PEI-PEG-DUPA (PPD), comprising polyethylenimine-polyethyleneglycol (PEI-PEG) tethered to the PSMA ligand, 2-[3-(1, 3-dicarboxy propyl)ureido] pentanedioic acid (DUPA), to treat PC. The purpose of PEI is to bind polyinosinic/polycytosinic acid (polyIC) and allow endosomal release, while DUPA targets PC cells. PolyIC activates multiple pathways that lead to tumor cell death and to the activation of bystander effects that harness the immune system against the tumor, attacking nontargeted neighboring tumor cells and reducing the probability of acquired resistance and disease recurrence...
December 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29229808/impact-of-insecticide-resistance-in-anopheles-arabiensis-on-malaria-incidence-and-prevalence-in-sudan-and-the-costs-of-mitigation
#20
Hmooda Toto Kafy, Bashir Adam Ismail, Abraham Peter Mnzava, Jonathan Lines, Mogahid Shiekh Eldin Abdin, Jihad Sulieman Eltaher, Anuar Osman Banaga, Philippa West, John Bradley, Jackie Cook, Brent Thomas, Krishanthi Subramaniam, Janet Hemingway, Tessa Bellamy Knox, Elfatih M Malik, Joshua O Yukich, Martin James Donnelly, Immo Kleinschmidt
Insecticide-based interventions have contributed to ∼78% of the reduction in the malaria burden in sub-Saharan Africa since 2000. Insecticide resistance in malaria vectors could presage a catastrophic rebound in disease incidence and mortality. A major impediment to the implementation of insecticide resistance management strategies is that evidence of the impact of resistance on malaria disease burden is limited. A cluster randomized trial was conducted in Sudan with pyrethroid-resistant and carbamate-susceptible malaria vectors...
December 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
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