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https://www.readbyqxmd.com/read/29040929/determination-of-geographical-origin-and-icariin-content-of-herba-epimedii-using-near-infrared-spectroscopy-and-chemometrics
#1
Yue Yang, Yongjiang Wu, Weili Li, Xuesong Liu, Jiyu Zheng, Wentao Zhang, Yong Chen
Near infrared (NIR) spectroscopy coupled with chemometrics was used to discriminate the geographical origin of Herba Epimedii in this work. Four different classification models, namely discriminant analysis (DA), back propagation neural network (BPNN), K-nearest neighbor (KNN), and support vector machine (SVM), were constructed, and their performances in terms of recognition accuracy were compared. The results indicated that the SVM model was superior over the other models in the geographical origin identification of Herba Epimedii...
October 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29040908/predicting-lysine-glycation-sites-using-bi-profile-bayes-feature-extraction
#2
Zhe Ju, Juhe Sun, Yanjie Li, Li Wang
Glycation is a nonenzymatic post-translational modification which has been found to be involved in various biological processes and closely associated with many metabolic diseases. The accurate identification of glycation sites is important to understand the underlying molecular mechanisms of glycation. As the traditional experimental methods are often labor-intensive and time-consuming, it is desired to develop computational methods to predict glycation sites. In this study, a novel predictor named BPB_GlySite is proposed to predict lysine glycation sites by using bi-profile bayes feature extraction and support vector machine algorithm...
October 12, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29040803/synthesis-and-characterization-of-fatty-acid-grafted-chitosan-polymer-and-their-nanomicelles-for-non-viral-gene-delivery-applications
#3
Divya Sharma, Jagdish Singh
The aim of this study was to synthesize and characterize fatty acid-grafted-chitosan (fatty acid-g-CS) polymer and their nanomicelles for using as carriers for gene delivery. CS was hydrophobically modified using saturated fatty acids of increasing fatty acyl chain length. Carbodiimide along with N-hydroxysuccinimide was used for coupling carboxyl group of fatty acids with amine groups of CS. Proton nuclear magnetic resonance and Fourier transform-infrared spectroscopy were used to quantify fatty acyl substitution onto CS backbone...
October 17, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29040730/diurnal-temperature-range-and-chikungunya-virus-infection-in-invasive-mosquito-vectors
#4
Barry W Alto, Keenan Wiggins, Bradley Eastmond, Sara Ortiz, Kylie Zirbel, L Philip Lounibos
Climate strongly influences the geographic distribution and timing of mosquito-borne disease outbreaks. Environmental temperature affects phenotypic traits of mosquitoes including vector competence for arboviruses mediated by changes in infection, extrinsic incubation period and in rates of transmission. Most experiments, however, are done at constant temperatures. In nature, mosquitoes are more likely to experience daily fluctuations in temperature. Here we compare disseminated infection (leg infection) and saliva infection of Aedes aegypti (L...
October 10, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29040704/in-planta-processing-of-the-spcas9-grna-complex
#5
Masafumi Mikami, Seiichi Toki, Masaki Endo
In CRISPR/Cas9-mediated genome editing in plants, Streptococcus pyogenes Cas9 (SpCas9) protein and the required guide RNA (gRNA) are, in most cases, expressed from a stably integrated transgene. Generally, SpCas9 protein is expressed from an RNA polymerase (pol) II promoter, while gRNA is expressed from a pol III promoter. However, pol III promoters have not been much characterized other than in model plants, making it difficult to select appropriate promoters for specific applications, while pol II transcripts have to be processed to generate functional gRNAs...
October 12, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29040681/20-years-of-the-smart-protein-domain-annotation-resource
#6
Ivica Letunic, Peer Bork
SMART (Simple Modular Architecture Research Tool) is a web resource (http://smart.embl.de) for the identification and annotation of protein domains and the analysis of protein domain architectures. SMART version 8 contains manually curated models for more than 1300 protein domains, with approximately 100 new models added since our last update article (1). The underlying protein databases were synchronized with UniProt (2), Ensembl (3) and STRING (4), doubling the total number of annotated domains and other protein features to more than 200 million...
October 10, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29040672/unsupervised-learning-of-spatiotemporal-interictal-discharges-in-focal-epilepsy
#7
Maxime O Baud, Jonathan K Kleen, Gopala K Anumanchipalli, Liberty S Hamilton, Yee-Leng Tan, Robert Knowlton, Edward F Chang
BACKGROUND: Interictal epileptiform discharges are an important biomarker for localization of focal epilepsy, especially in patients who undergo chronic intracranial monitoring. Manual detection of these pathophysiological events is cumbersome, but is still superior to current rule-based approaches in most automated algorithms. OBJECTIVE: To develop an unsupervised machine-learning algorithm for the improved, automated detection and localization of interictal epileptiform discharges based on spatiotemporal pattern recognition...
October 10, 2017: Neurosurgery
https://www.readbyqxmd.com/read/29040629/elongation-factor-1%C3%AE-accurately-reconstructs-relationships-amongst-psyllid-families-hemiptera-psylloidea-with-possible-diagnostic-implications
#8
Francesco Martoni, Simon R Bulman, Andrew Pitman, Karen F Armstrong
The superfamily Psylloidea (Hemiptera: Sternorrhyncha) lacks a robust multigene phylogeny. This impedes our understanding of the evolution of this group of insects and, consequently, an accurate identification of individuals, of their plant host associations, and their roles as vectors of economically important plant pathogens. The conserved nuclear gene elongation factor-1 alpha (EF-1α) has been valuable as a higher-level phylogenetic marker in insects and it has also been widely used to investigate the evolution of intron/exon structure...
October 10, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29040362/prediction-of-protein-protein-interactions-between-fungus-magnaporthe-grisea-and-rice-oryza-sativa-l
#9
Shiwei Ma, Qi Song, Huan Tao, Andrew Harrison, Shaobo Wang, Wei Liu, Shoukai Lin, Ziding Zhang, Yufang Ai, Huaqin He
Rice blast disease caused by the fungus Magnaporthe grisea (M. grisea) is one of the most serious diseases for the cultivated rice Oryza sativa (O. sativa). A key factor causing rice blast disease and defense might be protein-protein interactions (PPIs) between rice and fungus. In this research, we have developed a computational pipeline to predict PPIs between blast fungus and rice. After cross-prediction by interolog-based and domain-based method, we achieved 532 potential PPIs between 27 fungus proteins and 236 rice proteins...
October 11, 2017: Briefings in Bioinformatics
https://www.readbyqxmd.com/read/29040305/switching-and-optimizing-control-for-coal-flotation-process-based-on-a-hybrid-model
#10
Zhiyong Dong, Ranfeng Wang, Minqiang Fan, Xiang Fu
Flotation is an important part of coal preparation, and the flotation column is widely applied as efficient flotation equipment. This process is complex and affected by many factors, with the froth depth and reagent dosage being two of the most important and frequently manipulated variables. This paper proposes a new method of switching and optimizing control for the coal flotation process. A hybrid model is built and evaluated using industrial data. First, wavelet analysis and principal component analysis (PCA) are applied for signal pre-processing...
2017: PloS One
https://www.readbyqxmd.com/read/29040267/protecting-cows-in-small-holder-farms-in-east-africa-from-tsetse-flies-by-mimicking-the-odor-profile-of-a-non-host-bovid
#11
Rajinder K Saini, Benedict O Orindi, Norber Mbahin, John A Andoke, Peter N Muasa, David M Mbuvi, Caroline M Muya, John A Pickett, Christian W Borgemeister
BACKGROUND: For the first time, differential attraction of pathogen vectors to vertebrate animals is investigated for novel repellents which when applied to preferred host animals turn them into non-hosts thereby providing a new paradigm for innovative vector control. For effectively controlling tsetse flies (Glossina spp.), vectors of African trypanosomosis, causing nagana, repellents more powerful than plant derived, from a non-host animal the waterbuck, Kobus ellipsiprymnus defassa, have recently been identified...
October 17, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29040159/new-approach-for-differentiation-of-bone-marrow-mesenchymal-stem-cells-toward-chondrocyte-cells-with-overexpression-of-microrna-140
#12
Mahboudi Hossein, Soleimani Masoud, Hanaee-Ahvaz Hana, Ghanbarian Hossein, Bandehpour Mojgan, Enderami Seyed Ehsan, Kazemi Bahram
Mesenchymal stem cells are widely stimulated by transforming growth factor beta-3 (TGFβ3) for chondrocyte differentiation. The objective of our study was to establish a new method for differentiation of human mesenchymal stem cells toward chondrocyte by overexpression of MicroRNA-140 (miR-140), and also this method was compared with method of induction with TGFβ3 in high-cell density culture systems. Mesenchymal stem cells were harvested from bone marrow of human. We prepared vectors and then was used for recombinant Lenti virus production in HEK-293 cell...
October 16, 2017: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/29036807/obtaining-and-characterization-of-monoclonal-antibodies-against-recombinant-extracellular-domain-of-human-epidermal-growth-factor-receptor-2
#13
Sarina Nurgul, Abeldenov Sailau, Turgimbayeva Aigerim, Zhylkibayev Assylbek, Ramankulov Erlan, Khassenov Bekbolat, Eskendirova Saule
Human epidermal growth factor receptor 2 (HER2) is an important biomarker for detection and treatment of different types of cancers such as breast, ovarian, stomach cancer. In this study, we developed a monoclonal antibody against the extracellular domain (ECD) of HER2 biomarker of breast cancer. For this purpose, the ECD-HER2 gene was amplified and cloned into an expression vector. Gene was generated in Escherichia coli BL21 (DE3) strain for expression of recombinant protein. The expressed protein was separated by SDS-PAGE and detected by anti-his monoclonal antibody in immunoblotting...
September 8, 2017: Human Antibodies
https://www.readbyqxmd.com/read/29036788/knockdown-of-lncrna-ccat2-inhibits-endometrial-cancer-cells-growth-and-metastasis-via-sponging-mir-216b
#14
Pengmu Xie, Hongying Cao, Ying Li, Jianhua Wang, Zhumei Cui
OBJECTIVE: Colon cancer-associated transcript 2 (CCAT2), a novel lncRNA has been reported as an oncogene in several cancers. This study was aimed to explore whether CCAT2 also exerted oncogenic roles in endometrial cancer cells. MATERIALS AND METHODS: The expression of CCAT2 in 30 pairs of endometrial cancer and matched non-cancerous tissues were detected by qRT-PCR. Two endometrial cancer cell lines HEC-1-A and RL95-2 were used throughout this study. CCAT2 in cells was silenced by transfection with shRNA targeted CCAT2, then cell growth and metastasis were assessed by performing trypan blue staining, Transwell assay, and flow cytometry...
September 29, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/29036573/complexviewer-visualization-of-curated-macromolecular-complexes
#15
Colin W Combe, Marine Dumousseau Sivade, Henning Hermjakob, Joshua Heimbach, Birgit H M Meldal, Gos Micklem, Sandra Orchard, Juri Rappsilber
Summary: Proteins frequently function as parts of complexes, assemblages of multiple proteins and other biomolecules, yet network visualizations usually only show proteins as parts of binary interactions. ComplexViewer visualizes interactions with more than two participants and thereby avoids the need to first expand these into multiple binary interactions. Furthermore, if binding regions between molecules are known then these can be displayed in the context of the larger complex. Availability and implementation: freely available under Apache version 2 license; EMBL-EBI Complex Portal: http://www...
August 3, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29036474/casanova-a-multiclass-support-vector-machine-model-for-the-classification-of-human-sperm-motility-patterns
#16
Summer G Goodson, Sarah White, Alicia M Stevans, Sanjana Bhat, Chia-Yu Kao, Scott Jaworski, Tamara R Marlowe, Martin Kohlmeier, Leonard McMillan, Steven H Zeisel, Deborah A O'Brien
The ability to accurately monitor alterations in sperm motility is paramount to understanding multiple genetic and biochemical perturbations impacting normal fertilization. Computer-aided sperm analysis (CASA) of human sperm typically reports motile percentage and kinematic parameters at the population level, and uses kinematic gating methods to identify subpopulations such as progressive or hyperactivated sperm. The goal of this study was to develop an automated method that classifies all patterns of human sperm motility during in vitro capacitation following the removal of seminal plasma...
October 4, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29036390/endothelial-cells-modified-by-adenovirus-vector-containing-nine-copies-hypoxia-response-elements-and-human-vascular-endothelial-growth-factor-as-the-novel-seed-cells-for-bone-tissue-engineering
#17
Xiaobin Song, Liang Shi, Lamei Chen, Xinyu Liu, Xun Qu, Ketao Wang, Fengcai Wei
Vascularization is one of the hotspots during the development of new therapeutic strategies for bone tissue engineering, which can alleviate hypoxic circumstance and prevent transplant failure. Vascular endothelial growth factor (VEGF) gene transfection using recombinant adenovirus (Ad) vector can effectively promote angiogenesis, but uncontrolled long-term continuous expression of VEGF brings safety concern. Here we constructed a recombinant Ad vector containing nine copies of HRE promoter and the hVEGF165 gene, which conserved the oxygen sensitivity of hypoxia-inducible factor-1/hypoxia response elements (HIF-1/HRE)...
September 27, 2017: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/29036370/estimation-of-the-effects-of-multipoint-pacing-on-battery-longevity-in-routine-clinical-practice
#18
Finn Akerström, Irene Narváez, Alberto Puchol, Marta Pachón, Cristina Martín-Sierra, Moisés Rodríguez-Mañero, Luis Rodríguez-Padial, Miguel A Arias
Aims: Multipoint pacing (MPP) permits simultaneous multisite pacing of the left ventricle (LV); initial studies suggest haemodynamic and clinical benefits over conventional (single LV site) cardiac resynchronization therapy (CRT). The aim of this study was to estimate the impact of MPP activation on battery longevity in routine clinical practice. Methods and results: Patient (n = 46) and device data were collected from two centres at least 3 months after MPP-CRT device implantation...
September 23, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/29036170/quantifying-the-effects-of-temperature-on-mosquito-and-parasite-traits-that-determine-the-transmission-potential-of-human-malaria
#19
Lillian L M Shapiro, Shelley A Whitehead, Matthew B Thomas
Malaria transmission is known to be strongly impacted by temperature. The current understanding of how temperature affects mosquito and parasite life history traits derives from a limited number of empirical studies. These studies, some dating back to the early part of last century, are often poorly controlled, have limited replication, explore a narrow range of temperatures, and use a mixture of parasite and mosquito species. Here, we use a single pairing of the Asian mosquito vector, An. stephensi and the human malaria parasite, P...
October 16, 2017: PLoS Biology
https://www.readbyqxmd.com/read/29036169/developing-a-dengue-forecast-model-using-machine-learning-a-case-study-in-china
#20
Pi Guo, Tao Liu, Qin Zhang, Li Wang, Jianpeng Xiao, Qingying Zhang, Ganfeng Luo, Zhihao Li, Jianfeng He, Yonghui Zhang, Wenjun Ma
BACKGROUND: In China, dengue remains an important public health issue with expanded areas and increased incidence recently. Accurate and timely forecasts of dengue incidence in China are still lacking. We aimed to use the state-of-the-art machine learning algorithms to develop an accurate predictive model of dengue. METHODOLOGY/PRINCIPAL FINDINGS: Weekly dengue cases, Baidu search queries and climate factors (mean temperature, relative humidity and rainfall) during 2011-2014 in Guangdong were gathered...
October 16, 2017: PLoS Neglected Tropical Diseases
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