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https://www.readbyqxmd.com/read/28454463/reduced-ebp50-expression-levels-are-correlated-with-unfavorable-clinicopathological-features-of-extrahepatic-bile-duct-carcinoma-and-promote-the-proliferation-and-migration-of-qbc939-cells
#1
Duiping Feng, Ying Xiong, Zhiqiang Peng, Qiang Ma, Tao Tao, Hua Liu, Jianfang Liang, Zhigang Wei, Junfang Zheng, Lei Wang, Hui Zhang
The present study aimed to clarify the association between ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50) expression level and the tumor phenotype and clinicopathological features of extrahepatic bile duct carcinoma. Tissue samples from patients with extrahepatic bile duct carcinoma (54 cases) and patients with normal bile duct epithelia from gallbladder of cholecystitis (20 cases) were collected, and immunohistochemical staining was used to detect the expression levels of EBP50 in these tissues. In addition, small interfering (si)RNA-EBP50 was used to knock down the expression of EBP50 in the QBC939 human cholangiocarcinoma (CC) cell line...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454403/mir-22-promotes-apoptosis-of-osteosarcoma-cells-via-inducing-cell-cycle-arrest
#2
Pengzhou Gai, Hongliang Sun, Guangda Wang, Qiang Xu, Xiaojun Qi, Zuofu Zhang, Lei Jiang
To study the effects of miR-22 on the proliferation and the apoptosis of osteosarcoma MG-63 cell line and to explore the potential molecular mechanism that miR-22 regulates this biological process. Quantitive real-time polymerase chain reaction (RT-qPCR) was performed to explore the miRNA level of miR-22. The MG-63 cell line was infected with miR-22 mimics for establishment of miR-22 overexpression. Non-infected cells were in blank group and cells infected with empty vector were served as negative control (NC group)...
April 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454319/construction-and-expression-of-a-lentivirus-expression-vector-carrying-the-vegf165-egfp-fusion-gene-in-breast-cancer-mcf-7-cells
#3
Minna Luo, Huan Huang, Lei Hou, Shan Shao, Shangke Huang, Xinhan Zhao
Vascular endothelial growth factor (VEGF)165 is one of the most abundant and potent angiogenic factors in both physiological and pathological conditions. However, the function and mechanism of VEGF165 in tumors and their environment remain to be elucidated. In the present study, a lentivirus vector (LV) that contained the VEGF165-enhanced green fluorescent protein (EGFP) fusion gene was constructed and transfected into the human breast cancer cell line MCF-7. Following transfection, the expression of VEGF165 in MCF-7 cells was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454290/a-multi-shrna-vector-enhances-the-silencing-efficiency-of-exogenous-and-endogenous-genes-in-human-cells
#4
Yanjie Weng, Ying Shi, Xi Xia, Wenjuan Zhou, Hongyan Wang, Changyu Wang
RNA interference (RNAi) is a powerful technology for suppressing gene function. In most studies, small interfering RNAs (siRNAs) consist of one short hairpin RNA (shRNA) and, therefore, are often unable to achieve loss-of-function of their target genes. In the current study, an RNAi vector containing three shRNAs under the control of three RNA polymerase III U6 promoters was constructed. RNAi vectors containing one or two shRNAs were generated for comparisons. A pilot study targeting exogenously expressed DsRed in the HEK293 cell line revealed promising effects and a high selectivity for the multi-shRNA RNAi vector...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454226/long-non-coding-rna-hotair-enhances-radioresistance-in-mda-mb231-breast-cancer-cells
#5
Yun Zhou, Chaoqun Wang, Xia Liu, Chengjun Wu, Haitao Yin
The aim of the present study was to investigate the radiosensitizing effects of homeobox (HOX) transcript antisense RNA (HOTAIR) long non-coding RNA on breast cancer tumor cells and examine the underlying mechanisms. Recombinant plasmid vectors containing HOTAIR gene were constructed and MDA-MB231 cells were transfected with these plasmids using liposomes. The cells were treated with radiation and cell apoptosis, proliferation, and double-stranded DNA breaks were examined. HOXD10, phosphorylated AKT (p-AKT) and p-BAD expression levels were measured using western blot analysis...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454222/effects-of-rna-silencing-of-matrix-metalloproteinase-2-on-the-growth-of-esophageal-carcinoma-cells-in-vivo
#6
Yu-Guang Shen, Wen Feng, Yi-Jun Xu, Na-Na Jiao, Da-Qiang Sun, Wen-Dong Qu, Quan Tang, Wei Xiong, Yang Tang, Yu Xia, Qing-Yong Cai, Da-Xing Liu, Xun Zhang, Gang Xu, Gui-You Liang
Esophageal carcinoma is one of the most common malignancies in China. Previous studies reported that matrix metalloproteinases (MMPs) have important roles in the progression and invasion of numerous types of solid tumors. Among the MMPs, MMP-2 has been closely associated with tumor growth and invasion. In the present study, a short hairpin RNA (shRNA) lentiviral expression vector targeting the MMP-2 gene was constructed in order to observe the inhibitory effect of MMP-2 gene silencing on the growth of the KYSE150 esophageal carcinoma cell line in vivo...
March 2017: Oncology Letters
https://www.readbyqxmd.com/read/28454088/overexpression-of-a-functional-calcium-sensing-receptor-dramatically-increases-osteolytic-potential-of-mda-mb-231-cells-in-a-mouse-model-of-bone-metastasis-through-epiregulin-mediated-osteoprotegerin-downregulation
#7
Boudot Cédric, Hénaut Lucie, Thiem Ursula, Geraci Sandra, Galante Mariangela, Saldanha Paulo, Saidak Zuzana, Six Isabelle, Clézardin Philippe, Kamel Said, Mentaverri Romuald
INTRODUCTION AND AIMS: Osteolytic bone metastases are observed in advanced cases of breast cancer. In vitro data suggest that the activity of the calcium-sensing receptor (CaSR) expressed by metastatic cells could potentiate their osteolytic potential. This study aimed to demonstrate in vivo the involvement of the CaSR in breast cancer cells osteolytic potential and to identify potential targets linked to CaSR activity. METHODS AND RESULTS: MDA-MB-231 stably transfected with plasmids containing either a full-length wild-type CaSR (CaSR-WT), or a functionally inactive dominant negative mutant (CaSR-DN) or an empty vector (EV) were intratibially injected into Balb/c-Nude mice...
April 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28453911/validation-of-biomechanical-deformable-image-registration-in-the-abdomen-thorax-and-pelvis-in-a-commercial-radiotherapy-treatment-planning-system
#8
Michael Velec, Joanne L Moseley, Stina Svensson, Björn Hårdemark, David A Jaffray, Kristy K Brock
PURPOSE: The accuracy of deformable image registration tools can vary widely between imaging modalities and specific implementations of the same algorithms. A biomechanical model-based algorithm initially developed in-house at an academic institution was translated into a commercial radiotherapy treatment planning system and validated for multiple imaging modalities and anatomic sites. METHODS: Biomechanical deformable registration (Morfeus) is a geometry driven algorithm based on the finite element method...
April 28, 2017: Medical Physics
https://www.readbyqxmd.com/read/28453837/borrelia-burgdorferi-bbi39-paralogs-targets-of-protective-immunity-reduce-pathogen-persistence-either-in-hosts-or-in-the-vector
#9
Preeti Singh, Deepshikha Verma, Brian T Backstedt, Simarjot Kaur, Manish Kumar, Alexis A Smith, Kavita Sharma, Xiuli Yang, José F Azevedo, Maria Gomes-Solecki, Ozlem Buyuktanir, Utpal Pal
Borrelia burgdorferi genome harbors several paralogous gene families (pgf) that can encode immunogenic proteins of unknown function. Protein-protein interaction assays using a transmission-blocking vaccine candidate, BBA52, as bait identified an interacting partner in spirochetes-a member of pgf 54, annotated as BBI39. We show that BBI39 is a surface-exposed membrane antigen that is immunogenic during spirochete infection, despite the gene being primarily transcribed in the vector with a transient expression in the host only at tick-bite sites...
March 15, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28453776/modulating-and-evaluating-receptor-promiscuity-through-directed-evolution-and-modeling
#10
Sarah C Stainbrook, Jessica S Yu, Michael P Reddick, Neda Bagheri, Keith E J Tyo
The promiscuity of G-protein-coupled receptors (GPCRs) has broad implications in disease, pharmacology and biosensing. Promiscuity is a particularly crucial consideration for protein engineering, where the ability to modulate and model promiscuity is essential for developing desirable proteins. Here, we present methodologies for (i) modifying GPCR promiscuity using directed evolution and (ii) predicting receptor response and identifying important peptide features using quantitative structure-activity relationship models and grouping-exhaustive feature selection...
April 27, 2017: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/28453576/a-novel-framework-for-the-identification-of-drug-target-proteins-combining-stacked-auto-encoders-with-a-biased-support-vector-machine
#11
Qi Wang, YangHe Feng, JinCai Huang, TengJiao Wang, GuangQuan Cheng
The identification of drug target proteins (IDTP) plays a critical role in biometrics. The aim of this study was to retrieve potential drug target proteins (DTPs) from a collected protein dataset, which represents an overwhelming task of great significance. Previously reported methodologies for this task generally employ protein-protein interactive networks but neglect informative biochemical attributes. We formulated a novel framework utilizing biochemical attributes to address this problem. In the framework, a biased support vector machine (BSVM) was combined with the deep embedded representation extracted using a deep learning model, stacked auto-encoders (SAEs)...
2017: PloS One
https://www.readbyqxmd.com/read/28453513/genetic-diversity-and-population-structure-of-the-tsetse-fly-glossina-fuscipes-fuscipes-diptera-glossinidae-in-northern-uganda-implications-for-vector-control
#12
Robert Opiro, Norah P Saarman, Richard Echodu, Elizabeth A Opiyo, Kirstin Dion, Alexis Halyard, Augustine W Dunn, Serap Aksoy, Adalgisa Caccone
Uganda is the only country where the chronic and acute forms of human African Trypanosomiasis (HAT) or sleeping sickness both occur and are separated by < 100 km in areas north of Lake Kyoga. In Uganda, Glossina fuscipes fuscipes is the main vector of the Trypanosoma parasites responsible for these diseases as well for the animal African Trypanosomiasis (AAT), or Nagana. We used highly polymorphic microsatellite loci and a mitochondrial DNA (mtDNA) marker to provide fine scale spatial resolution of genetic structure of G...
April 28, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28453368/establishment-of-expanded-and-streamlined-pipeline-of-pitch-knock-in-a-web-based-design-tool-for-mmej-mediated-gene-knock-in-pitch-designer-and-the-variations-of-pitch-pitch-tg-and-pitch-kiko
#13
Kazuki Nakamae, Yuki Nishimura, Mitsumasa Takenaga, Shota Nakade, Naoaki Sakamoto, Hiroshi Ide, Tetsushi Sakuma, Takashi Yamamoto
The emerging genome editing technology has enabled the creation of gene knock-in cells easily, efficiently, and rapidly, which has dramatically accelerated research in the field of mammalian functional genomics, including in humans. We recently developed a microhomology-mediated end-joining-based gene knock-in method, termed the PITCh system, and presented various examples of its application. Since the PITCh system only requires very short microhomologies (up to 40 bp) and single-guide RNA target sites on the donor vector, the targeting construct can be rapidly prepared compared with the conventional targeting vector for homologous recombination-based knock-in...
April 28, 2017: Bioengineered
https://www.readbyqxmd.com/read/28453362/a-novel-mutation-in-the-dominantly-inherited-topors-gene-supports-haploinsufficiency-as-the-mechanism-of-retinitis-pigmentosa
#14
Marta Latasiewicz, Anna Paola Salvetti, Robert E MacLaren
BACKGROUND: Inherited retinal degenerations are a major cause of untreatable blindness in the younger age group. Recent advances in gene therapy using adeno-associated viral (AAV) vectors have raised the possibility of slowing or stopping retinal degenerations with gene replacement in cases of gene deficiency. MATERIALS AND METHODS: In this report, we present a family with autosomal dominant retinitis pigmentosa. A screen for common ADRP genes was performed with 105 genes targeted...
April 28, 2017: Ophthalmic Genetics
https://www.readbyqxmd.com/read/28453272/bacterial-iron-uptake-pathways-gates-for-the-import-of-bactericide-compounds
#15
Isabelle J Schalk, Gaëtan L A Mislin
Bacterial resistance to most antibiotics in clinical use has reached alarming proportions. A challenge for modern medicine will be to discover new antibiotics or strategies to combat multidrug resistant bacteria, especially Gram-negative bacteria for which the situation is particularly critical. Vectorization of bactericide compounds by siderophores (iron chelators produced by bacteria) is a promising strategy able to considerably increase the efficacy of drugs. Such a Trojan horse strategy can also extend activity of specific Gram-positive antibiotics to Gram-negative bacteria...
April 28, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28453254/gene-transfection-mediated-by-catiomers-requires-free-highly-charged-polymer-chains-to-overcome-intracellular-barriers
#16
Lindomar Jose Calumby Albuquerque, Carlos E de Castro, Karin A Riske, Maria Cristina Carlan da Silva, Paulo I R Muraro, Vanessa Schmidt Giacomelli, Cristiano Giacomelli, Fernando Carlos Carlos Giacomelli
The prospective use of the block copolymers PEO113-b-PDEA50 and POEGMA70-b-P(OEGMA10-co-DEA47-co-DPA47) as non-viral gene vectors was evaluated. The polymers are able to properly condense DNA into nano-sized particles (RH ~ 75 nm) which are marginally cytotoxic and can be uptaken by cells. However, the GFP expression assays evidenced that DNA delivery is essentially negligible meaning that intracellular trafficking hampers efficient gene release. Subsequently we demonstrate that cellular uptake and particularly the quantity of GFP-positive cells are substantially enhanced when the block copolymer polyplexes are produced and further supplemented by BPEI chains (branched polyethyleneimine)...
April 28, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28449534/safety-profile-of-egfr-targeted-hybrid-vector-system-composed-of-pamam-dendrimer-and-oncolytic-adenovirus
#17
COMMENT
A-Rum Yoon, Dayananda Kasala, Jinwoo Hong, Chae-Ok Yun
No abstract text is available yet for this article.
March 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/28449201/silencing-of-agrobacterium-tumefaciens-oncogenes-ipt-and-iaam-induces-resistance-to-crown-gall-disease-in-plum-but-not-in-apricot
#18
Nuria Alburquerque, Lydia Faize, Lorenzo Burgos
BACKGROUND: In this study two vectors with short-length chimeric transgenes were used to produce Prunus rootstocks resistant to crown gall disease through RNA interference-mediated gene silencing of the Agrobacterium tumefaciens oncogenes ipt and iaaM. RESULTS: Transgenic plum and apricot lines were produced with efficiencies of up to 7.7 and 1.1%, respectively. An in vitro evaluation method allowed identifying susceptible lines and reducing the number of lines to be evaluated in the greenhouse...
April 27, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28449112/phenotypic-variability-of-the-amazonian-species-rhodnius-brethesi-hemiptera-reduviidae
#19
A C Souza, S Catalá, A L Carbajal de la Fuente, A C V Junqueira
The wild species Rhodnius brethesi (Matta, 1919) (Hemiptera: Reduviidae) is found in areas of piassabais in microregion of Rio Negro, Amazonas, Brazil. Its geographical distribution overlaps the areas of Leopoldinia piassaba palm. In areas where palm trees are found, transmission cycle of Trypanosoma cruzi is related to the extractive activity of the palm fiber, exposing workers to wild vector transmission of this parasite. The close association with the palm tree L. piassaba suggests that this wild triatomine has special features in its sensory system allowing specificity of ecotope...
April 25, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28449102/enhancing-expression-of-ssu1-genes-in-saccharomyces-uvarum-leads-to-an-increase-in-sulfite-tolerance-and-transcriptome-profiles-change
#20
X Z Liu, M Sang, X A Zhang, T K Zhang, H Y Zhang, X He, S X Li, X D Sun, Z M Zhang
Saccharomyces uvarum is a good wine yeast species which may have great potential for the future. However, sulfur tolerance of most S. uvarum strains is very poor. In addition there is still little information about the SSU1 gene of S. uvarum. In order to analyze the function of SSU1 genes, two expression vectors which contained different SSU1 genes were constructed and transferred into a sulfite-tolerant S. uvarum strain A9. Then sulfite tolerance, PCR analysis, sequencing analysis, SO2 output production analysis, RT-qPCR analysis and transcriptome analysis were used to access the function of the S...
April 25, 2017: FEMS Yeast Research
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