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https://www.readbyqxmd.com/read/28654862/biopolymer-zein-coated-gold-nanoparticles-synthesis-antibacterial-potential-toxicity-and-histopathological-effects-against-the-zika-virus-vector-aedes-aegypti
#1
Periyakaruppan Suganya, Baskaralingam Vaseeharan, Sekar Vijayakumar, Banumathi Balan, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
The control of multidrug-resistant bacteria as well as insect pests and vectors is timely and important now a days. The present study was designed to evaluate the in vitro antibacterial, antibiofilm and mosquito larvicidal effects of gold nanoparticles synthesized using the zein biopolymer (Ze-AuNPs) against Gram positive (Bacillus pumilus and Bacillus subtilis), Gram negative (Shigella sonnei and Pseudomonas aeruginosa) bacteria and third instar larvae of the dengue and Zika virus vector Aedes aegypti. The synthesized Ze-AuNPs were characterized by UV-vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM)...
June 17, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28654784/generation-and-characterization-of-a-human-oral-squamous-carcinoma-cell-line-scc-9-with-crispr-cas9-mediated-deletion-of-the-p75-neurotrophin-receptor
#2
Ping Huang, Dongdong Tong, Jing Sun, Qing Li, Fenghe Zhang
OBJECTIVE: To investigate the importance of the p75 neurotrophin receptor (p75(NTR)) in human tongue squamous carcinoma cells, we exploited the CRISPR/Cas9 technology to establish a p75(NTR)-knockout SCC-9 cell line and to explore the effect on biological functions. MATERIALS AND METHODS: The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonuclease (Cas9) system was used to generate genomic deletion mutants of p75(NTR) in the tongue squamous carcinoma cell lines SCC-9...
June 24, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28654693/fatty-acid-synthase-reprograms-the-epigenome-in-uterine-leiomyosarcomas
#3
Min Guan, Xiwei Wu, Peiguo Chu, Warren A Chow
SK-UT-1 uterine leiomyosarcomas (Ut-LMS) cells were transduced with a fatty acid synthase (FASN)-containing retroviral vector to recapitulate the "lipogenic phenotype of cancer." Consistent with this model, forced expression of FASN enhanced SK-UT-1 proliferation, migration, and cellular motion. Further investigation showed FASN promotes trimethylation of H3K9 (H3K9me3) and acetylation of H3K27 (H3K27ac) in SK-UT-1 cells. In contrast, siRNA targeting of FASN in high endogenous FASN expressing SK-LMS-1 Ut-LMS cells inhibits trimethylation of H3K9 and acetylation of H3K27...
2017: PloS One
https://www.readbyqxmd.com/read/28654423/daily-wrist-activity-classification-using-a-smart-band
#4
Nhan Duc Nguyen, Phuc Huu Truong, Gu-Min Jeong
In this letter, we propose a novel method for classifying daily wrist activities by using a smart band. Triaxial acceleration data are collected by built-in sensors of the smart band during experiments regarding five activities, i.e., texting, calling, placing a hand in a pocket, carrying a suitcase, and swinging a hand. We analyze patterns in the sensor signals during these activities based on three types of features, i.e., norm, norm-variance, and frequency-domain features. After extracting the significant features, a multi-class support vector machine (SVM) algorithm is applied to classify these activities...
June 27, 2017: Physiological Measurement
https://www.readbyqxmd.com/read/28654319/partial-characterization-of-tick-borne-encephalitis-virus-isolates-from-ticks-of-southern-ukraine
#5
Oksana O Yurchenko, Dmytro O Dubina, Nataliya O Vynograd, Jean-Paul Gonzalez
Tick-borne encephalitis (TBE) is the most common tick-borne viral infection in Eurasia; thousands of human cases are annually reported from several European countries. Several tick species are vectors of the tick-borne encephalitis virus (TBEV), while TBE appears to be spreading from the Eurasian continent westward to Europe. Fifteen study sites were chosen from five territories of southern Ukraine, including Odessa, Mykolaiv, Kherson Oblast, the Autonomous Republic of Crimea, and Sevastopol. Tick collection was performed in spring season of three consecutive years (1988-1990) using either flagging technique or direct collection of specimens feeding on cattle...
June 27, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28654073/rapid-deletion-production-in-fungi-via-agrobacterium-mediated-transformation-of-oscar-deletion-constructs
#6
Scott E Gold, Zahi Paz, María D García-Pedrajas, Anthony E Glenn
Precise deletion of gene(s) of interest, while leaving the rest of the genome unchanged, provides the ideal product to determine that particular gene's function in the living organism. In this protocol the OSCAR method of precise and rapid deletion plasmid construction is described. OSCAR relies on the cloning system in which a single recombinase reaction is carried out containing the purified PCR-amplified 5' and 3' flanks of the gene of interest and two plasmids, pA-Hyg OSCAR (the marker vector) and pOSCAR (the assembly vector)...
June 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28654071/protocols-for-c-brick-dna-standard-assembly-using-cpf1
#7
Shi-Yuan Li, Guo-Ping Zhao, Jin Wang
CRISPR-associated protein Cpf1 cleaves double-stranded DNA under the guidance of CRISPR RNA (crRNA), generating sticky ends. Because of this characteristic, Cpf1 has been used for the establishment of a DNA assembly standard called C-Brick, which has the advantage of long recognition sites and short scars. On a standard C-Brick vector, there are four Cpf1 recognition sites - the prefix (T1 and T2 sites) and the suffix (T3 and T4 sites) - flanking biological DNA parts. The cleavage of T2 and T3 sites produces complementary sticky ends, which allow for the assembly of DNA parts with T2 and T3 sites...
June 15, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28654067/bidirectional-retroviral-integration-site-pcr-methodology-and-quantitative-data-analysis-workflow
#8
Gajendra W Suryawanshi, Song Xu, Yiming Xie, Tom Chou, Namshin Kim, Irvin S Y Chen, Sanggu Kim
Integration Site (IS) assays are a critical component of the study of retroviral integration sites and their biological significance. In recent retroviral gene therapy studies, IS assays, in combination with next-generation sequencing, have been used as a cell-tracking tool to characterize clonal stem cell populations sharing the same IS. For the accurate comparison of repopulating stem cell clones within and across different samples, the detection sensitivity, data reproducibility, and high-throughput capacity of the assay are among the most important assay qualities...
June 14, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28654036/in-ovo-electroporation-in-the-chicken-auditory-brainstem
#9
Ting Lu, Ariel Loren Cohen, Jason Tait Sanchez
Electroporation is a method that introduces genes of interest into biologically relevant organisms like the chicken embryo. It is long established that the chicken embryo is an effective research model for studying basic biological functions of auditory system development. More recently, the chicken embryo has become particularly valuable in studying gene expression, regulation and function associated with hearing. In ovo electroporation can be used to target auditory brainstem regions responsible for highly specialized auditory functions...
June 9, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28653683/vector-control-gets-new-impetus-and-direction
#10
EDITORIAL
(no author information available yet)
No abstract text is available yet for this article.
June 27, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28653659/continuous-assessment-of-schizophrenia-using-heart-rate-and-accelerometer-data
#11
Erik Reinertsen, Maxim Osipov, Chengyu Liu, John M Kane, Georgios Petrides, Gari D Clifford
OBJECTIVE: Schizophrenia has been associated with changes in heart rate (HR) and physical activity measures. However, the relationship between analysis window length and classifier accuracy using these features has yet to be quantified. APPROACH: Here we used objective HR and activity data to classify contiguous days of data as belonging to a schizophrenia patient or a healthy control. HR and physical activity recordings were made on 12 medicated subjects with schizophrenia and 12 healthy controls...
June 27, 2017: Physiological Measurement
https://www.readbyqxmd.com/read/28653623/replication-study-the-common-feature-of-leukemia-associated-idh1-and-idh2-mutations-is-a-neomorphic-enzyme-activity-converting-alpha-ketoglutarate-to-2-hydroxyglutarate
#12
Megan Reed Showalter, Jason Hatakeyama, Tomas Cajka, Kacey VanderVorst, Kermit L Carraway, Oliver Fiehn
In 2016, as part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Fiehn et al., 2016), that described how we intended to replicate selected experiments from the paper "The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting alpha-ketoglutarate to 2-hydroxyglutarate" (Ward et al., 2010). Here, we report the results of those experiments. We found that cells expressing R172K mutant IDH2 did not display isocitrate-dependent NADPH production above vector control levels, in contrast to the increased production observed with wild-type IDH2...
June 27, 2017: ELife
https://www.readbyqxmd.com/read/28653538/cationic-polypeptoids-with-optimized-molecular-characteristics-toward-efficient-non-viral-gene-delivery
#13
Lipeng Zhu, Jessica M Simpson, Xin Xu, Hua He, Donghui Zhang, Lichen Yin
The rational design of gene vectors relies on the understanding of their structure-property relationship. Polypeptoids, structural isomers of natural polypeptides, hold great potentials as gene delivery vectors due to their facial preparation, structural tunability, and most importantly, desired proteolytic stability. We herein designed a library of polypeptoids with different cationic side-chain terminal groups, degrees of polymerization (DP), side-chain lengths, and incorporated aliphatic side chains, attempting to unravel the structure-property relationship toward maximized gene delivery efficiency while minimized cytotoxicity...
June 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28653450/environmental-risk-modeling-and-potential-sand-fly-vectors-of-cutaneous-leishmaniasis-in-chitral-district-a-leishmanial-focal-point-of-mount-tirchmir-pakistan
#14
Farrah Zaidi, Syeda Hira Fatima, Tehmina Jan, Mehreen Fatima, Asad Ali, Muhammad Khisroon, Muhammad Adnan, Syed Basit Rasheed
OBJECTIVE: To provide baseline information about suspected vectors and the incidence, distribution and an active zone of transmission for Cutaneous Leishmaniasis (CL) in Chitral, Pakistan using GIS tools; and to investigate the role of environmental factors in the disease dynamics. METHOD: Two surveys in 2014 and 2016 as a basis for chloropleth and environmental risk mapping. RESULTS: 769 captured specimens yielded 18 Phlebotomus and 4 Sergentomyia species including two potential vectors of CL, i...
June 27, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28653334/vector-competence-of-malaysian-aedes-albopictus-with-and-without-wolbachia-to-four-dengue-virus-serotypes
#15
Sylvia Joanne, Indra Vythilingam, Boon-Teong Teoh, Cherng-Shii Leong, Kim-Kee Tan, Meng-Li Wong, Nava Yugavathy, Sazaly AbuBakar
OBJECTIVE: To determine the susceptibility status of Aedes albopictus with and without Wolbachia to the four dengue virus serotypes. METHODS: Two newly colonised colonies of Ae. albopictus from the wild were used for the study. One colony was naturally infected with Wolbachia while in the other Wolbachia was removed by tetracycline treatment. Both colonies were orally infected with dengue virus-infected fresh blood meal. Dengue virus load was measured using quantitative RT-PCR at four time intervals in the salivary glands, midguts and ovaries...
June 27, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28653015/classification-of-breast-masses-in-ultrasound-images-using-self-adaptive-differential-evolution-extreme-learning-machine-and-rough-set-feature-selection
#16
Kadayanallur Mahadevan Prabusankarlal, Palanisamy Thirumoorthy, Radhakrishnan Manavalan
A method using rough set feature selection and extreme learning machine (ELM) whose learning strategy and hidden node parameters are optimized by self-adaptive differential evolution (SaDE) algorithm for classification of breast masses is investigated. A pathologically proven database of 140 breast ultrasound images, including 80 benign and 60 malignant, is used for this study. A fast nonlocal means algorithm is applied for speckle noise removal, and multiresolution analysis of undecimated discrete wavelet transform is used for accurate segmentation of breast lesions...
April 2017: Journal of Medical Imaging
https://www.readbyqxmd.com/read/28652859/lncrna-h19-promotes-the-differentiation-of-bovine-skeletal-muscle-satellite-cells-by-suppressing-sirt1-foxo1
#17
Xiaochun Xu, Shengyue Ji, Weili Li, Bao Yi, Hengxin Li, Hongfu Zhang, Wenping Ma
BACKGROUND: H19 is a well-characterized Long noncoding RNA (lncRNA) that has been proven to promote myoblast differentiation in humans and mice. However, its mechanism of action is still not fully interpreted. METHODS: Using RT-qPCR, we examined H19 RNA levels in various tissues from 1-week, 1-month, 6-month and 36-month old male cattle (i.e., newborn, infant, young and adult). The protein and mRNA levels of MyoG, MyHC, Sirt1 and FoxO1 in the satellite and C2C12 cells with an H19 silencing or overexpression vector were respectively detected using western blot and real-time qPCR...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28651889/a-novel-expression-vector-for-the-secretion-of-abaecin-in-bacillus-subtilis
#18
Li Li, Lan Mu, Xiaojuan Wang, Jingfeng Yu, Ruiping Hu, Zhen Li
This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant...
June 3, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28651635/an-anatomical-like-triangular-vector-ligament-reconstruction-for-the-medial-collateral-ligament-and-the-posterior-oblique-ligament-injury-with-single-femoral-tunnel-a-retrospective-study
#19
Hongtao Xu, Kai Kang, Jian Zhang, Dongmei Xin, Wei Liu, Guorong Jin, Jiangtao Dong, Shijun Gao
BACKGROUND: The purpose of this study was to evaluate the clinical outcomes of anatomical-like triangular-vector ligament reconstruction (TLR) in treating the combined injury of medial collateral ligament (MCL) and posterior oblique ligament (POL). METHODS: During July 2013 to May 2014, 26 patients who received anatomical-like TLR were included into this study. All patients received clinical physical examination, imaging examination, and knee joint function score both preoperative and follow-up...
June 26, 2017: Journal of Orthopaedic Surgery and Research
https://www.readbyqxmd.com/read/28651628/separability-of-motor-imagery-of-the-self-from-interpretation-of-motor-intentions-of-others-at-the-single-trial-level-an-eeg-study
#20
João Andrade, José Cecílio, Marco Simões, Francisco Sales, Miguel Castelo-Branco
BACKGROUND: We aimed to investigate the separability of the neural correlates of 2 types of motor imagery, self and third person (actions owned by the participant himself vs. another individual). If possible this would allow for the development of BCI interfaces to train disorders of action and intention understanding beyond simple imitation, such as autism. METHODS: We used EEG recordings from 20 healthy participants, as well as electrocorticography (ECoG) in one, based on a virtual reality setup...
June 26, 2017: Journal of Neuroengineering and Rehabilitation
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