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https://www.readbyqxmd.com/read/28256631/spore-surface-proteins-of-brevibacillus-laterosporus-are-involved-in-insect-pathogenesis
#1
Maria Giovanna Marche, Maria Elena Mura, Giovanni Falchi, Luca Ruiu
Outer spore envelope proteins of pathogenic bacteria often present specific virulence factors and tools to evade the defence system of their hosts. Brevibacillus laterosporus, a pathogen of invertebrates and an antimicrobial-producing species, is characterised by a unique spore coat and canoe-shaped parasporal body (SC-CSPB) complex surrounding the core spore. In the present study, we identified and characterised major proteins of the SC-CSPB complex of B. laterosporus, and we investigated their entomopathogenic role...
March 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28122880/lethal-effects-of-the-insect-growth-regulator-cyromazine-against-three-species-of-filth-flies-musca-domestica-stomoxys-calcitrans-and-fannia-canicularis-diptera-muscidae-in-cattle-swine-and-chicken-manure
#2
William A Donahue, Allan T Showler, Michael W Donahue, Bret E Vinson, Weste L A Osbrink
The presence of various species of filth flies is a widespread problem where livestock, including poultry, are maintained and where manure accumulates. The house fly, Musca domestica L.; the stable fly, Stomoxys calcitrans (L.); and the little house fly, Fannia canicularis (L.) (each Diptera: Muscidae), the target pests in our study, can mechanically spread diseases, and S. calcitrans can bite cattle, causing losses in meat and milk production. Chemical control is widely used to suppress filth flies, but resistance to conventional insecticides has become problematic...
January 25, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28102959/x-ray-scatter-correction-for-multi-source-interior-computed-tomography
#3
Hao Gong, Hao Yan, Xun Jia, Bin Li, Ge Wang, Guohua Cao
PURPOSE: The schemes of multi-source interior computed tomography (CT) have shown promise for ultra-fast, organ-oriented, and low-dose dynamic imaging. Besides forward scattering, x-ray cross scattering from multiple x-ray sources activated simultaneously can further degrade image quality. Here, we investigate the overall x-ray scattering artifact in a recently proposed multi-source interior CT architecture, and present two methods for scatter correction. METHODS: Compared to single-source global CT, scattering in multi-source interior CT architecture is affected by two new factors: cross scattering from simultaneously activated multiple x-ray sources and region-of-interest (ROI) oriented interior CT mode...
January 2017: Medical Physics
https://www.readbyqxmd.com/read/28087775/rapid-expansion-of-immune-related-gene-families-in-the-house-fly-musca-domestica
#4
Timothy B Sackton, Brian P Lazzaro, Andrew G Clark
The house fly, Musca domestica, occupies an unusual diversity of potentially septic niches compared to other sequenced Dipteran insects and is a vector of numerous diseases of humans and livestock. In the present study, we apply whole-transcriptome sequencing to identify genes whose expression is regulated in adult flies upon bacterial infection. We then combine the transcriptomic data with analysis of rates of gene duplication and loss to provide insight into the evolutionary dynamics of immune-related genes...
January 12, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28073977/the-effects-of-a-modified-hot-water-treatment-on-anastrepha-ludens-diptera-tephritidae-infested-mango
#5
Emilio Hernández, Marysol Aceituno-Medina, Jorge Toledo, Bigail Bravo, José Caro-Corrales, Pablo Montoya, Robert Mangan
The Mexican fruit fly, Anastrepha ludens (Loew), is a quarantine pest in mango (Mangifera indica L.) that can be controlled by using a hot water treatment (HWT). This treatment is normally followed by a 30-min hydrocooling (HYC) process that reduces the negative effects that the treatment has on fruit quality. However, if hot water-treated fruits are immediately immersed in water at 21 °C, the survival rate of third-instar A. ludens may be increased. The current approved treatment protocol states that if HYC is used, then treated fruit should undergo an additional 10-min HWT or on platform for 30 min before HYC...
January 10, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28048554/th-ab-202-03-a-novel-tool-for-computing-deliverable-doses-in-dynamic-mlc-tracking-treatments
#6
M Fast, C Kamerling, S Crijns, M Menten, S Nill, B Raaymakers, U Oelfke
PURPOSE: In tracked dynamic multi-leaf collimator (MLC) treatments, segments are continuously adapted to the target centroid motion in beams-eye-view. On-the-fly segment adaptation, however, potentially induces dosimetric errors due to the finite MLC leaf width and non-rigid target motion. In this study, we outline a novel tool for computing the 4d dose of lung SBRT plans delivered with MLC tracking. METHODS: The following automated workflow was developed: A) centroid tracking, where the initial segments are morphed to each 4dCT phase based on the beams-eye-view GTV shift (followed by a dose calculation on each phase); B) re-optimized tracking, in which all morphed initial plans from (A) are further optimised ("warm-started") in each 4dCT phase using the initial optimisation parameters but phase-specific volume definitions...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28039199/occupational-allergy-to-fruit-flies-drosophila-melanogaster-in-laboratory-workers
#7
Meinir Jones, Sue Blair, Stephanie MacNeill, Jennifer Welch, Alice Hole, Peter Baxter, Paul Cullinan
OBJECTIVES: Drosophila melanogaster (the 'fruit fly') is commonly used in genetic research, but there is only one report of IgE-associated allergy in exposed workers. 4 newly identified cases prompted us to examine the extent of this problem in a university laboratory. Our aim in this study is to determine the prevalence and determinants of sensitisation to fruit flies in a population of exposed workers. METHODS: In a cross-sectional study, we surveyed 286 employees working in a department carrying out research involving D...
December 30, 2016: Occupational and Environmental Medicine
https://www.readbyqxmd.com/read/28031470/effectiveness-of-naturally-occurring-substances-added-to-duck-litter-in-reducing-emergence-and-landing-of-adult-musca-domestica-diptera-muscidae
#8
Simon Lachance, Justine Shiell, Michele T Guerin, Cynthia Scott-Dupree
Poultry barns provide an ideal breeding environment for Musca domestica L. (Diptera: Muscidae) owing to the controlled temperatures and litter (manure plus bedding material) accumulation. Management techniques that render the litter less suitable for immature stage development, such as the addition of naturally occurring insecticidal substances, could reduce fly infestations. The effectiveness of acetic, boric, and citric acids; diatomaceous earth; and hydrated lime at restricting female landing and growth of house flies in duck litter was evaluated...
December 28, 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28028097/behavior-and-survival-of-the-filth-fly-parasitoids-spalangia-endius-and-urolepis-rufipes-hymenoptera-pteromalidae-in-response-to-three-granular-house-fly-baits-and-components
#9
Edwin R Burgess, B H King
Behaviors and mortality of two filth fly parasitoid wasps, Spalangia endius Walker and Urolepis rufipes Ashmead, were tested in response to granular fly baits containing one of the three active ingredients (AI): Golden Malrin (methomyl), QuickBayt (imidacloprid), or Quikstrike (dinotefuran). Behavioral responses to each of the two components of the baits, the AIs and the fly attractant pheromone (Z)-9-tricosene, were also examined independently. Spalangia endius avoided contact with bait granules, regardless of bait type...
December 2016: Environmental Entomology
https://www.readbyqxmd.com/read/28025247/prevalence-of-escherichia-coli-o157-h7-from-house-flies-diptera-muscidae-and-dairy-samples-in-north-central-florida1
#10
Roxanne G Burrus, Jerome A Hogsette, Phillip E Kaufman, James E Maruniak, Amy H Simonne, Volker Mai
Efficient detection of enterohemorrhagic Escherichia coli O157:H7 is important to monitor the safety of food products obtained from cattle, and it has been primarily accomplished by analyzing manure samples by selective cultivation techniques, PCR, and ELISA. As each technique suffers from different biases, there may be value in using multiple methods and samples to increase detection efficiency. Difficulties associated with cattle manure sampling can be circumvented by isolation of E. coli O157:H7 from house flies, Musca domestica (L...
December 25, 2016: Journal of Medical Entomology
https://www.readbyqxmd.com/read/27936090/manure-preferences-and-postemergence-learning-of-two-filth-fly-parasitoids-spalangia-cameroni-and-muscidifurax-raptor-hymenoptera-pteromalidae
#11
Caitlin E Taylor, Erika T Machtinger, Christopher J Geden, Matthew Kramer
The efficiency of host-seeking behavior is crucial to the reproductive performance of female parasitoids. Initially, parasitoids may use chemical information garnered from the microhabitat in which they emerge to locate hosts. Spalangia cameroni and Muscidifurax raptor are commercially available parasitoids of filth flies. Postemergence exposure to a specific manure may provide a way to increase parasitism in specific microhabitats found at livestock facilities upon release. In this study, female parasitoids of both species were exposed to equine manure, bovine manure, or clean pupae...
2016: PloS One
https://www.readbyqxmd.com/read/27925019/entering-and-exiting-behaviour-of-the-phlebotomine-sand-fly-lutzomyia-longiflocosa-diptera-psychodidae-in-rural-houses-of-the-sub-andean-region-of-colombia
#12
Raúl Hernando Pardo, Erika Santamaría, Olga Lucia Cabrera
The present study identified the entering and exiting sites for Lutzomyia longiflocosa in rural houses of the sub-Andean region in Colombia. Entering sites were identified with sticky traps set up outside the bedrooms, around the eave openings, and with cage traps enclosing the slits in the doors and windows inside the bedrooms. Exiting sites were identified by releasing groups of females indoors. These females were blood fed and marked with fluorescent powders. Females were recaptured with the trap placement described above but set up on the opposite sides of the openings...
January 1, 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/27899608/tfbsbank-a-platform-to-dissect-the-big-data-of-protein-dna-interaction-in-human-and-model-species
#13
Dongsheng Chen, Sanjie Jiang, Xiaoyan Ma, Fang Li
Genome-wide transcription factors (TFs) binding data has been extensively generated in the past few years, which poses a great challenge to data interpretation. Therefore, comprehensive and dedicated functional annotation databases for TF-DNA interaction are in great demands to manage, explore and utilize those invaluable data resources. Here, we constructed a platform 'TFBSbank' which houses the annotation of 1870 chromatin immunoprecipitation (ChIP) datasets of 585 TFs in five species (human, mouse, fly, worm and yeast)...
January 4, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/27876987/prevalence-of-hypodermosis-in-cattle-slaughtered-in-industrial-slaughtered-house-of-ardebil-iran
#14
Naghi Mohammad Bagherzadeh, Hamed Behniafar, Sadegh Rahbari, Soghra Valizadeh
Hypodermosis is one of the important veterinary problems because of its worldwide prevalence and significant economic losses. Economic losses is due to decreasing of productivity potential and leather quality. The disease is caused by two parasitic fly species in cattle, Hypoderma bovis and lineatus. Diagnosis of the parasites has crucial importance because of treatment and eradication plannings. Basically diagnosis is based on parasitological methods but recently serological methods were used in some countries...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27864339/development-of-a-platform-to-enable-fully-automated-cross-titration-experiments
#15
Jason Cassaday, Michael Finley, Brian Squadroni, Sylvie Jezequel-Sur, Albert Rauch, Bharti Gajera, Victor Uebele, Jeffrey Hermes, Paul Zuck
In the triage of hits from a high-throughput screening campaign or during the optimization of a lead compound, it is relatively routine to test compounds at multiple concentrations to determine potency and maximal effect. Additional follow-up experiments, such as agonist shift, can be quite valuable in ascertaining compound mechanism of action (MOA). However, these experiments require cross-titration of a test compound with the activating ligand of the receptor requiring 100-200 data points, severely limiting the number tested in MOA assays in a screening triage...
April 2017: SLAS Technol
https://www.readbyqxmd.com/read/27809930/sleeping-habits-affect-access-to-host-by-chagas-disease-vector-triatoma-dimidiata
#16
Etienne Waleckx, Rafael Pasos-Alquicira, María Jesús Ramírez-Sierra, Eric Dumonteil
BACKGROUND: Chagas disease, caused by the parasite Trypanosoma cruzi, is mainly transmitted by blood-sucking bugs called triatomines. In the Yucatán Peninsula, Mexico, the main vector of T. cruzi is Triatoma dimidiata. While this species may colonize houses in other regions, it is mostly intrusive in Yucatán: it generally lives in sylvan and peridomestic areas, and frequently enters inside homes, likely attracted by potential vertebrate hosts, without establishing colonies. Bugs collected inside homes have a low nutritional status, suggesting that they cannot efficiently feed inside these houses...
November 3, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27792261/diversity-of-knockdown-resistance-alleles-in-a-single-house-fly-population-facilitates-adaptation-to-pyrethroid-insecticides
#17
S Kasai, H Sun, J G Scott
Insecticide use exerts a tremendous selection force on house fly populations, but the frequencies of the initial resistance mutations may not reach high levels if they have a significant fitness cost in the absence of insecticides. However, with the continued use of the same (or similar) insecticides, it is expected that new mutations (conferring equal or greater resistance, but less of a fitness cost) will evolve. Pyrethroid insecticides target the insect voltage sensitive sodium channel (VSSC) and have been widely used for control of house flies at animal production facilities for more than three decades...
February 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/27781284/effects-of-four-commercial-fungal-formulations-on-mortality-and-sporulation-in-house-flies-musca-domestica-and-stable-flies-stomoxys-calcitrans
#18
E N I Weeks, E T Machtinger, S A Gezan, P E Kaufman, C J Geden
The house fly Musca domestica L. (Diptera: Muscidae) and stable fly Stomoxys calcitrans (L.) (Diptera: Muscidae) are major pests of livestock. Biological control is an important tool in an integrated control framework. Increased mortality in filth flies has been documented with entomopathogenic fungi, several strains of which are commercially available. Three strains of Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Hypocreales: Cordycipitaceae) and one strain of Metarhizium brunneum (Petch) (Hypocreales: Clavicipitaceae) were tested in commercial formulations for pathogenicity against house flies and stable flies...
October 26, 2016: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/27730590/protocols-to-study-aging-in-drosophila
#19
Matthew D W Piper, Linda Partridge
The fruit fly Drosophila melanogaster offers a host of advantages for studying the biology of aging: a well-understood biology, a wide range of genetic reagents, well-defined dietary requirements, and a relatively short life span, with a median of ~80 days and maximum ~100 days. Several phenotypes can be used to assess the aging process, but the simplest and most widely used metric is length of life. Here we describe a standard life span assay for Drosophila housed on a simple sugar/yeast diet.
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27694431/behavior-and-survival-of-the-filth-fly-parasitoids-spalangia-endius-and-urolepis-rufipes-hymenoptera-pteromalidae-in-response-to-three-granular-house-fly-baits-and-components
#20
Edwin R Burgess, B H King
Behaviors and mortality of two filth fly parasitoid wasps, Spalangia endius Walker and Urolepis rufipes Ashmead, were tested in response to granular fly baits containing one of the three active ingredients (AI): Golden Malrin (methomyl), QuickBayt (imidacloprid), or Quikstrike (dinotefuran). Behavioral responses to each of the two components of the baits, the AIs and the fly attractant pheromone (Z)-9-tricosene, were also examined independently. Spalangia endius avoided contact with bait granules, regardless of bait type...
September 30, 2016: Environmental Entomology
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