keyword
MENU ▼
Read by QxMD icon Read
search

GARP

keyword
https://www.readbyqxmd.com/read/28207945/cd4-cd25-garp-tregs-display-a-compromised-suppressive-function-in-patients-with-dilated-cardiomyopathy
#1
Yuzhen Wei, Kunwu Yu, Hui Wei, Xin Su, Ruirui Zhu, Huairui Shi, Haitao Sun, Quan Luo, Wenbin Xu, Junhui Xiao, Yucheng Zhong, Qiutang Zeng
Dilated cardiomyopathy (DCM) is a lethal inflammatory heart disease and closely connected with dysfunction of the immune system. Glycoprotein -A repetitions predominant (GARP) expressed on activated CD4(+) T cells with suppressive activity has been established. This study aimed to investigate the frequency and function of circulating CD4(+) CD25(+) GARP(+) regulatory T cells (Tregs) in DCM. 45 DCM patients and 46 controls were enrolled in this study. There was a significant increase in peripheral Th1 and Th17 number and their related cytokines (IFN-γ, IL-17), and an obvious decrease in Treg number, TGF-β1 levels and the expression of FOXP3 and GARP in patients with DCM as compared with controls...
February 16, 2017: Immunology
https://www.readbyqxmd.com/read/28198469/garp2-accelerates-retinal-degeneration-in-rod-cgmp-gated-cation-channel-%C3%AE-subunit-knockout-mice
#2
Marci L DeRamus, Delores A Stacks, Youwen Zhang, Carrie E Huisingh, Gerald McGwin, Steven J Pittler
The Cngb1 locus-encoded β-subunit of rod cGMP-gated cation channel and associated glutamic acid rich proteins (GARPs) are required for phototransduction, disk morphogenesis, and rod structural integrity. To probe individual protein structure/function of the GARPs, we have characterized several transgenic mouse lines selectively restoring GARPs on a Cngb1 knockout (X1(-/-)) mouse background. Optical coherence tomography (OCT), light and transmission electron microscopy (TEM), and electroretinography (ERG) were used to analyze 6 genotypes including WT at three and ten weeks postnatal...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28150865/shifts-in-the-ecological-niche-of-lutzomyia-peruensis-under-climate-change-scenarios-in-peru
#3
D A Moo-Llanes, W Arque-Chunga, O Carmona-Castro, C Yañez-Arenas, H H Yañez-Trujillano, L Cheverría-Pacheco, C M Baak-Baak, A G Cáceres
The Peruvian Andes presents a climate suitable for many species of sandfly that are known vectors of leishmaniasis or bartonellosis, including Lutzomyia peruensis (Diptera: Psychodidae), among others. In the present study, occurrences data for Lu. peruensis were compiled from several items in the scientific literature from Peru published between 1927 and 2015. Based on these data, ecological niche models were constructed to predict spatial distributions using three algorithms [Support vector machine (SVM), the Genetic Algorithm for Rule-set Prediction (GARP) and Maximum Entropy (MaxEnt)]...
February 2, 2017: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/28040549/increased-peripheral-cd4-regulatory-t-cells-persist-after-successful-direct-acting-antiviral-treatment-of-chronic-hepatitis-c
#4
Bettina Langhans, Hans Dieter Nischalke, Benjamin Krämer, Annekristin Hausen, Leona Dold, Peer van Heteren, Robert Hüneburg, Jacob Nattermann, Christian P Strassburg, Ulrich Spengler
INTRODUCTION: CD4(+) regulatory T cells (Tregs) expand during chronic hepatitis C virus (HCV) infection, inhibit antiviral immunity and promote fibrosis. Directly acting antivirals (DAA) have revolutionized HCV therapy. However, it is unclear if Tregs are normalized after DAA-induced HCV elimination. METHODS: We analyzed Tregs before (=baseline), at end of therapy (EOT), 12 and 24 weeks (SVR12, SVR24) and long-term (51±14 weeks) after EOT in 26 genotype-1-infected patients successfully treated with sofosbuvir (SOF) plus IFN/ribavirin (n=12) and IFN-free DAA regimens (SOF plus daclatasvir or simeprevir; n=14)...
December 28, 2016: Journal of Hepatology
https://www.readbyqxmd.com/read/28039301/il-6-inhibits-upregulation-of-membrane-bound-tgf-%C3%AE-1-on-cd4-t-cells-and-blocking-il-6-enhances-oral-tolerance
#5
Chantal Kuhn, Rafael Machado Rezende, Hanane M'Hamdi, Andre Pires da Cunha, Howard L Weiner
Oral administration of Ag induces regulatory T cells that express latent membrane-bound TGF-β (latency-associated peptide [LAP]) and have been shown to play an important role in the induction of oral tolerance. We developed an in vitro model to study modulation of LAP(+) on CD4(+) T cells. The combination of anti-CD3 mAb, anti-CD28 mAb, and recombinant IL-2 induced expression of LAP on naive CD4(+) T cells, independent of Foxp3 or exogenous TGF-β. In vitro generated CD4(+)LAP(+)Foxp3(-) T cells were suppressive in vitro, inhibiting proliferation of naive CD4(+) T cells and IL-17A secretion by Th17 cells...
December 30, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28025245/predicting-the-distribution-of-phlebotomus-papatasi-diptera-psychodidae-the-primary-vector-of-zoonotic-cutaneous-leishmaniasis-in-golestan-province-of-iran-using-ecological-niche-modeling-comparison-of-maxent-and-garp-models
#6
Aioub Sofizadeh, Yavar Rassi, Hassan Vatandoost, Ahmad Ali Hanafi-Bojd, Abolfazl Mollalo, Sayena Rafizadeh, Amir Ahmad Akhavan
Zoonotic cutaneous leishmaniasis (ZCL) is a prevalent vector-borne disease in the Golestan province of Iran, with Phlebotomus papatasi (Scopoli, 1786) serving as the main vector. The aim of this study was to model the probability of presence of this species in the study area, and to determine the underlying factors affecting its distribution. Three villages were selected from each county of the province and visited monthly for investigating ZCL. Sticky paper traps were used for collecting the sand flies to determine the species present...
December 24, 2016: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28001437/garp-as-a-therapeutic-target-for-modulation-of-t-regulatory-cell-function
#7
Ethan M Shevach
Foxp3(+) T regulatory cells (Tregs) play critical roles in immune homeostasis primarily by suppressing many aspects of the immune response. Tregs uniquely express GARP on their cell surface and GARP functions as a delivery system for latent TGF-β. As Treg-derived TGF-β may mediate the suppressive functions of Tregs, GARP may represent a target to inhibit Treg suppression in cancer or augment suppression in autoimmunity. Areas covered: This article will focus on 1) the role of Treg-derived TGF-β in the suppressive activity of Treg, 2) the cellular and molecular regulation of expression of GARP on mouse and human Tregs, 3) the role of integrins in the activation of latent-TGF-β/GARP complex, 4) an overview of our present understanding of the function of the latent-TGF-β/GARP complex...
December 29, 2016: Expert Opinion on Therapeutic Targets
https://www.readbyqxmd.com/read/27956610/neutrophils-induce-proangiogenic-t-cells-with-a-regulatory-phenotype-in-pregnancy
#8
Suchita Nadkarni, Joanne Smith, Amanda N Sferruzzi-Perri, Agata Ledwozyw, Madhav Kishore, Robert Haas, Claudio Mauro, David J Williams, Sandra H P Farsky, Federica M Marelli-Berg, Mauro Perretti
Although neutrophils are known to be fundamental in controlling innate immune responses, their role in regulating adaptive immunity is just starting to be appreciated. We report that human neutrophils exposed to pregnancy hormones progesterone and estriol promote the establishment of maternal tolerance through the induction of a population of CD4(+) T cells displaying a GARP(+)CD127(lo)FOXP3(+) phenotype following antigen activation. Neutrophil-induced T (niT) cells produce IL-10, IL-17, and VEGF and promote vessel growth in vitro...
December 27, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27929418/yeast-reporter-assay-to-identify-cellular-components-of-ricin-toxin-a-chain-trafficking
#9
Björn Becker, Tina Schnöder, Manfred J Schmitt
RTA, the catalytic A-subunit of the ribosome inactivating A/B toxin ricin, inhibits eukaryotic protein biosynthesis by depurination of 28S rRNA. Although cell surface binding of ricin holotoxin is mainly mediated through its B-subunit (RTB), sole application of RTA is also toxic, albeit to a significantly lower extent, suggesting alternative pathways for toxin uptake and transport. Since ricin toxin trafficking in mammalian cells is still not fully understood, we developed a GFP-based reporter assay in yeast that allows rapid identification of cellular components required for RTA uptake and subsequent transport through a target cell...
December 6, 2016: Toxins
https://www.readbyqxmd.com/read/27913437/surface-expression-of-tgf%C3%AE-docking-receptor-garp-promotes-oncogenesis-and-immune-tolerance-in-breast-cancer
#10
Alessandra Metelli, Bill X Wu, Caroline W Fugle, Saleh Rachidi, Shaoli Sun, Yongliang Zhang, Jennifer Wu, Stephen Tomlinson, Philip H Howe, Yi Yang, Elizabeth Garrett-Mayer, Bei Liu, Zihai Li
GARP encoded by the Lrrc32 gene is the cell surface docking receptor for latent TGFβ, which is expressed naturally by platelets and regulatory T cells (Treg). Although Lrrc32 is amplified frequently in breast cancer, the expression and relevant functions of GARP in cancer have not been explored. Here, we report that GARP exerts oncogenic effects, promoting immune tolerance by enriching and activating latent TGFβ in the tumor microenvironment. We found that human breast, lung, and colon cancers expressed GARP aberrantly...
December 15, 2016: Cancer Research
https://www.readbyqxmd.com/read/27884290/atopic-dermatitis-according-to-garp-new-mechanistic-insights-in-disease-pathogenesis
#11
Janna Nousbeck, Alan D Irvine
In complex disease such as atopic dermatitis, the journey from identification of strong risk loci to profound functional and mechanistic insights can take several years. Here, Manz et al. have elegantly deciphered the mechanistic pathways in the well-established 11q13.5 atopic dermatitis risk locus. Their genetic and functional insights emphasize a role for T regulatory cells in atopic dermatitis pathogenesis.
December 2016: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/27777305/expansion-of-lysine-rich-repeats-in-plasmodium-proteins-generates-novel-localization-sequences-that-target-the-periphery-of-the-host-erythrocyte
#12
Heledd M Davies, Konstantinos Thalassinos, Andrew R Osborne
Repetitive low complexity sequences, mostly assumed to have no function, are common in proteins that are exported by the malaria parasite into its host erythrocyte. We identify a group of exported proteins containing short lysine-rich tandemly repeated sequences that are sufficient to localize to the erythrocyte periphery, where key virulence-related modifications to the plasma membrane and the underlying cytoskeleton are known to occur. Efficiency of targeting is dependent on repeat number, indicating that novel targeting modules could evolve by expansion of short lysine-rich sequences...
December 9, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27757121/golden-2-like-transcription-factors-of-plants
#13
Min Chen, Meiling Ji, Binbin Wen, Li Liu, Shaoxuan Li, Xiude Chen, Dongsheng Gao, Ling Li
Golden2-like (GLK) transcription factors are members of the GARP family of Myb transcription factors with an established relationship to chloroplast development in the plant kingdom. In the last century, Golden2 was proposed as a second golden producing factor and identified as controlling cellular differentiation in maize leaves. Then, GLKs were also found to play roles in disease defense and their function is conserved in regulating chloroplast development. Recently, research on GLKs has rapidly increased and shown that GLKs control chloroplast development in green and non-green tissues...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27581988/vaccinia-virus-uses-retromer-independent-cellular-retrograde-transport-pathways-to-facilitate-the-wrapping-of-intracellular-mature-virions-during-viral-morphogenesis
#14
Kate Harrison, Ismar R Haga, Tali Pechenick Jowers, Seema Jasim, Jean-Christophe Cintrat, Daniel Gillet, Thomas Schmitt-John, Paul Digard, Philippa M Beard
: Poxviruses such as Vaccinia virus (VACV) undertake a complex cytoplasmic replication cycle which involves morphogenesis through four distinct virion forms, and includes a crucial "wrapping" step whereby intracellular mature virions (IMVs) are wrapped in two additional membranes to form intracellular enveloped virions (IEVs). To determine if cellular retrograde transport pathways were required for this wrapping step we examined VACV morphogenesis in cells with reduced expression of the tetrameric tethering factor complex GARP (Golgi-associated retrograde pathway complex), a central component of retrograde transport...
August 31, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27462928/autophagy-in-saccharomyces-cerevisiae-requires-the-monomeric-gtp-binding-proteins-arl1-and-ypt6
#15
Shu Yang, Anne G Rosenwald
Macroautophagy/autophagy is a cellular degradation process that sequesters organelles or proteins into a double-membrane structure called the phagophore; this transient compartment matures into an autophagosome, which then fuses with the lysosome or vacuole to allow hydrolysis of the cargo. Factors that control membrane traffic are also essential for each step of autophagy. Here we demonstrate that 2 monomeric GTP-binding proteins in Saccharomyces cerevisiae, Arl1 and Ypt6, which belong to the Arf/Arl/Sar protein family and the Rab family, respectively, and control endosome-trans-Golgi traffic, are also necessary for starvation-induced autophagy under high temperature stress...
October 2, 2016: Autophagy
https://www.readbyqxmd.com/read/27458219/divergent-transcriptional-responses-to-physiological-and-xenobiotic-stress-in-giardia-duodenalis
#16
Brendan R E Ansell, Malcolm J McConville, Louise Baker, Pasi K Korhonen, Samantha J Emery, Staffan G Svärd, Robin B Gasser, Aaron R Jex
Understanding how parasites respond to stress can help to identify essential biological processes. Giardia duodenalis is a parasitic protist that infects the human gastrointestinal tract and causes 200 to 300 million cases of diarrhea annually. Metronidazole, a major antigiardial drug, is thought to cause oxidative damage within the infective trophozoite form. However, treatment efficacy is suboptimal, due partly to metronidazole-resistant infections. To elucidate conserved and stress-specific responses, we calibrated sublethal metronidazole, hydrogen peroxide, and thermal stresses to exert approximately equal pressure on trophozoite growth and compared transcriptional responses after 24 h of exposure...
October 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27448748/targeted-resequencing-and-functional-testing-identifies-low-frequency-missense-variants-in-the-gene-encoding-garp-as-significant-contributors-to-atopic-dermatitis-risk
#17
Judith Manz, Elke Rodríguez, Abdou ElSharawy, Eva-Maria Oesau, Britt-Sabina Petersen, Hansjörg Baurecht, Gabriele Mayr, Susanne Weber, Jürgen Harder, Eva Reischl, Agatha Schwarz, Natalija Novak, Andre Franke, Stephan Weidinger
Gene-mapping studies have consistently identified a susceptibility locus for atopic dermatitis and other inflammatory diseases on chromosome band 11q13.5, with the strongest association observed for a common variant located in an intergenic region between the two annotated genes C11orf30 and LRRC32. Using a targeted resequencing approach we identified low-frequency and rare missense mutations within the LRRC32 gene encoding the protein GARP, a receptor on activated regulatory T cells that binds latent transforming growth factor-β...
July 21, 2016: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/27440922/tssc1-is-novel-component-of-the-endosomal-retrieval-machinery
#18
David C Gershlick, Christina Schindler, Yu Chen, Juan S Bonifacino
Endosomes function as a hub for multiple protein-sorting events, including retrograde transport to the trans-Golgi network (TGN) and recycling to the plasma membrane. These processes are mediated by tubular-vesicular carriers that bud from early endosomes and fuse with a corresponding acceptor compartment. Two tethering complexes named GARP (composed of ANG2, VPS52, VPS53, and VPS54 subunits) and EARP (composed of ANG2, VPS52, VPS53, and Syndetin subunits) were previously shown to participate in SNARE-dependent fusion of endosome-derived carriers with the TGN and recycling endosomes, respectively...
September 15, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27428774/catchr-hops-and-corvet-tethering-complexes-share-a-similar-architecture
#19
Hui-Ting Chou, Danijela Dukovski, Melissa G Chambers, Karin M Reinisch, Thomas Walz
We show here that the Saccharomyces cerevisiae GARP complex and the Cog1-4 subcomplex of the COG complex, both members of the complexes associated with tethering containing helical rods (CATCHR) family of multisubunit tethering complexes, share the same subunit organization. We also show that HOPS, a tethering complex acting in the endolysosomal pathway, shares a similar architecture, thus suggesting that multisubunit tethering complexes use related structural frameworks.
August 2016: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/27415620/uncontrolled-il-17-production-by-intraepithelial-lymphocytes-in-a-case-of-non-ipex-autoimmune-enteropathy
#20
Moira Paroni, Andrea Magarotto, Silvia Tartari, Giulia Nizzoli, Paola Larghi, Giulia Ercoli, Umberto Gianelli, Massimiliano Pagani, Luca Elli, Sergio Abrignani, Dario Conte, Jens Geginat, Flavio Caprioli
OBJECTIVES: To provide a functional and phenotypic characterization of immune cells infiltrating small intestinal mucosa during non-IPEX autoimmune enteropathy (AIE), as to gain insights on the pathogenesis of this clinical condition. METHODS: Duodenal biopsies from a patient with AIE at baseline and following drug-induced remission were analyzed by immunohistochemistry, immunofluorescence, and flow cytometry, and results were compared with those obtained from patients with active celiac disease, ileal Crohn's disease and healthy controls...
2016: Clinical and Translational Gastroenterology
keyword
keyword
46471
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"