Eduardo Barbieri, Gina N Mollica, Brandyn D Moore, Sobhana A Sripada, Shriarjun Shastry, Ryan E Kilgore, Casee M Loudermilk, Zachary H Whitacre, Katie M Kilgour, Elena Wuestenhagen, Annika Aldinger, Heiner Graalfs, Oliver Rammo, Michael M Schulte, Thomas F Johnson, Michael A Daniele, Stefano Menegatti
The recent uptick in the approval of ex vivo cell therapies highlights the relevance of lentivirus (LV) as an enabling viral vector of modern medicine. As labile biologics, however, LVs pose critical challenges to industrial biomanufacturing. In particular, LV purification-currently reliant on filtration and anion-exchange or size-exclusion chromatography-suffers from long process times and low yield of transducing particles, which translate into high waiting time and cost to patients. Seeking to improve LV downstream processing, this study introduces peptides targeting the enveloped protein Vesicular stomatitis virus G (VSV-G) to serve as affinity ligands for the chromatographic purification of LV particles...
November 10, 2023: Biotechnology and Bioengineering