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Blood purification

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https://www.readbyqxmd.com/read/28105119/clinical-observation-on-the-treatment-of-acute-liver-failure-by-combined-non-biological-artificial-liver
#1
Maoqin Li, Jingxi Sun, Jiaqiong Li, Zaixiang Shi, Jiyuan Xu, Bo Lu, Shuli Cheng, Yanjun Xu, Xiaomeng Wang, Xianjiang Zhang
The clinical efficacy and safety of different combinations of non-bio artificial liver in the treatment of acute liver failure was examined. A total of 61 cases were selected under blood purification treatment from the patients with severe acute liver failure admitted to the severe disease department of the hospital from December, 2010 to December, 2015. Three types of artificial liver combinations were observed, i.e., plasma exchange plus hemoperfusion plus continuous venovenous hemodiafiltration (PE+HP+CVVHDF), PE+CVVHDF and HP+CVVHDF...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28101473/purification-and-characterization-of-bovine-serum-albumin-using-chromatographic-method
#2
Sanaz Balkani, Sara Shamekhi, Ramin Raoufinia, Reza Parvan, Jalal Abdolalizadeh
Purpose: Albumin is an abundant protein of blood and has many biopharmaceutical applications. The aim of this study was to purify bovine serum albumin (BSA) using produced rabbit anti-BSA antibody. Methods: The polyclonal antibody was produced against the BSA in rabbits. Then, the pure BSA was injected to three white New Zealand rabbits. ELISA test was done to evaluate antibody production. After antibody purification,the purified antibody was attached to CNBr-activated sepharose and finally it was used for purification of albumin from bovine serum...
December 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28078733/therapeutic-apheresis-in-immunologic-renal-and-neurological-diseases
#3
REVIEW
Rolf Bambauer, Reinhard Latza, Daniel Burgard, Ralf Schiel
Since the mid 1970s, when membrane modules became available, plasma separation techniques have gained in importance especially in the past few years. The advantages of this method are a complete separation of the corpuscular components from the plasma and due to increased blood flow rate and higher efficacy. Systemic autoimmune diseases based on an immune pathogenesis produce autoantibodies and circulating immune complexes, which cause inflammation in the tissues of various organs. In most cases, these diseases have a poor prognosis without treatment...
January 12, 2017: Therapeutic Apheresis and Dialysis
https://www.readbyqxmd.com/read/28073899/paper-based-microrna-expression-profiling-from-plasma-and-circulating-tumor-cells
#4
Sai Mun Leong, Karen Mei-Ling Tan, Hui Wen Chua, Mo-Chao Huang, Wai Chye Cheong, Mo-Huang Li, Steven Tucker, Evelyn Siew-Chuan Koay
BACKGROUND: Molecular characterization of circulating tumor cells (CTCs) holds great promise for monitoring metastatic progression and characterizing metastatic disease. However, leukocyte and red blood cell contamination of routinely isolated CTCs makes CTC-specific molecular characterization extremely challenging. METHODS: Here we report the use of a paper-based medium for efficient extraction of microRNAs (miRNAs) from limited amounts of biological samples such as rare CTCs harvested from cancer patient blood...
January 10, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28070048/-role-of-local-citrate-anticoagulation-in-continuous-blood-purification-to-patients-at-high-risk-of-bleeding-in-icu
#5
Shangping Zhao, Hao Ou, Yue Peng, Zuoliang Liu, Mingshi Yang, Xuefei Xiao
To evaluate the safety and efficiency of citrate anticoagulant-based continuous blood purification in patients at high risk of bleeding. 
 Methods: One hundred and fifty-two patients at high risk of bleeding were divided into local citrate group (group A, n=68) and heparin group (group B, n=84). Clotting function, change of pH, ionized sodium, bicarbonate ion, ionized calcium, activated clotting time (ACT) and complications were monitored before and during treatment. 
 Results: Compared to the group A, the incidence of clotting in filter and chamber, the degree of bleeding or fresh bleeding were significantly reduced in the group B (P<0...
December 28, 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28062629/unraveling-the-molecular-complexity-of-o-glycosylated-endogenous-n-terminal-pro-b-type-natriuretic-peptide-forms-in-blood-plasma-of-patients-with-severe-heart-failure
#6
Bernhard Halfinger, Angelika Hammerer-Lercher, Benno Amplatz, Bettina Sarg, Leopold Kremser, Herbert H Lindner
BACKGROUND: Currently, N-terminal pro-B-type natriuretic peptide (NT-proBNP) and its physiologically active counterpart, BNP, are most frequently used as biomarkers for diagnosis, prognosis, and disease monitoring of heart failure (HF). Commercial NT-proBNP and BNP immunoassays cross-react to varying degrees with unprocessed proBNP, which is also found in the circulation. ProBNP processing and immunoassay response are related to O-linked glycosylation of NT-proBNP and proBNP. There is a clear and urgent need to identify the glycosylation sites in the endogenously circulating peptides requested by the community to gain further insights into the different naturally occurring forms...
January 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28033679/peritoneal-dialysis-registry-with-2013-survey-report
#7
Ikuto Masakane, Takeshi Hasegawa, Satoshi Ogata, Naoki Kimata, Shigeru Nakai, Norio Hanafusa, Takayuki Hamano, Kenji Wakai, Atsushi Wada, Kosaku Nitta
Since 2009, the peritoneal dialysis (PD) registry has been carried out as part of the annual nationwide survey conducted by the Statistical Survey Committee of the Japanese Society for Dialysis Therapy with the cooperation of the Japanese Society for Peritoneal Dialysis. In this study, the current status of PD patients is reported on the basis of the results of the survey conducted at the end of 2013. The subjects were PD patients who lived in Japan and participated in the 2013 survey. Descriptive analysis was performed for various items including the current status of the combined use of PD and another dialysis method such as hemodialysis (HD) or hemodiafiltration (HDF), the method of exchanging dialysate, the use of an automated peritoneal dialysis (APD) machine, and the incidences of peritonitis and catheter exit-site infection...
December 2016: Therapeutic Apheresis and Dialysis
https://www.readbyqxmd.com/read/28013163/photocrosslinked-poly-amidoamine-nanoparticles-for-central-nervous-system-targeting
#8
Smbat Gevorgyan, Eleonora Rossi, Martino Alfredo Cappelluti, Alessandro Tocchio, Federico Martello, Irini Gerges, Cristina Lenardi, Paolo Milani, Simona Argentiere
This study presents an innovative method for the synthesis of polymeric nanoparticles (NPs) for central nervous system (CNS) targeting. The method is based on Ultraviolet light (UV)-induced crosslinking of diacrylamide-terminated oligomers of poly(amidoamine)s (PAAs), a widely used class of synthetic polymers in biomedical field research, especially in drug delivery thanks to their excellent biocompatibility and controlled biodegradability. Previous attempts aiming at preparing PAA-based NPs by self-assembly were challenged by lack of structural stability and consequently their early degradation and premature drug release...
December 14, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28002514/lectin-purification-from-carp-roe-cyprinus-carpio-l-investigation-of-its-carbohydrate-specificity-and-application-in-histochemistry
#9
Rostyslav Antonyuk, Alexander Lutsyk, Volodymyr Antonyuk
A method of lectin purification from carp roe (CCRA) was elaborated, which includes affinity chromatography on cross-linked ovomucoid and copolymers of polyvinyl alcohol and blood group-specific substances. That allowed obtaining lectin with electrophoretic purity and yielding of ˜ 42 mg÷kg roe. Electrophoresis in 15% polyacrylamide gel in the presence of β-mercaptoethanol showed one band with molecular mass ˜ 15 kDa, whereas in the absence of β-mercaptoethanol, CCRA exposed band with molecular mass ˜ 60 kDa...
2016: Romanian Journal of Morphology and Embryology, Revue Roumaine de Morphologie et Embryologie
https://www.readbyqxmd.com/read/28001351/proteoform-specific-protein-binding-of-small-molecules-in-complex-matrices
#10
Geuncheol Gil, Pan Mao, Bharathi Avula, Zulfiqar Ali, Amar G Chittiboyina, Ikhlas A Khan, Larry A Walker, Daojing Wang
Characterizing the specific binding between protein targets and small molecules is critically important for drug discovery. Conventional assays require isolation and purification of small molecules from complex matrices through multistep chromatographic fractionation, which may alter their original bioactivity. Most proteins undergo posttranslational modification, and only certain proteoforms have the right conformation with accessible domains and available residues for small molecule binding. We developed a top-down mass spectrometry (MS) centric workflow for rapid evaluation of the bioactivity of crude botanical extracts after a one-step reaction...
December 21, 2016: ACS Chemical Biology
https://www.readbyqxmd.com/read/28000366/hemocompatiblilty-evaluation-of-poly-1-8-octanediol-citrate-blend-polyethersulfone-membranes
#11
Muhamad Zulhilmi Zailani, Ahmad Fauzi Ismail, Siti Hamimah Sheikh Abdul Kadir, Mohd Hafiz Dzarfan Othman, Pei Sean Goh, Hasrinah Hasbullah, Mohd Sohaimi Abdullah, Be Cheer Ng, Fatmawati Kamal
In this study, poly (1,8- octanediol citrate) (POC) was used to modify polyethersufone (PES) based membrane to enhance its hemocompatibility. Different compositions of POC (0-3%) were added into the polyethersulfone (PES) dope solutions and polyvinylpyrrolidone (PVP) was used as pore forming agent. The hemocompatible POC modified PES membranes were fabricated through phase-inversion technique. The prepared membranes were characterized using attenuated total reflectance-fourier transform infrared (ATR-FTIR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), Atomic-force microscopy (AFM), contact angle, Zeta-potential, membrane porosity and pore size and pure water flux (PWF) and BSA rejection...
December 21, 2016: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27975084/slanted-asymmetric-microfluidic-lattices-as-size-selective-sieves-for-continuous-particle-cell-sorting
#12
Masumi Yamada, Wataru Seko, Takuma Yanai, Kasumi Ninomiya, Minoru Seki
Hydrodynamic microfluidic platforms have been proven to be useful and versatile for precisely sorting particles/cells based on their physicochemical properties. In this study, we demonstrate that a simple lattice-shaped microfluidic pattern can work as a virtual sieve for size-dependent continuous particle sorting. The lattice is composed of two types of microchannels ("main channels" and "separation channels"). These channels cross each other in a perpendicular fashion, and are slanted against the macroscopic flow direction...
December 15, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27951570/biocompatibility-of-hemodiafilters
#13
Tadashi Tomo
BACKGROUND: Biocompatibility and the efficiency of solute removal are important considerations in blood purification therapy. Improvement of biocompatibility is expected to lead to the prevention of dialysis-related complications (e.g. amyloidosis, arteriosclerosis, and malnutrition) and to the delay of disease progression by alleviating microinflammation. SUMMARY: The biocompatibility of dialyzers is greatly influenced by the interaction between blood and the treatment materials, in which the chemical and physical characteristics of membrane materials play important roles...
2017: Contributions to Nephrology
https://www.readbyqxmd.com/read/27951567/evidence-for-targeting-low-molecular-weight-proteins-in-hemodialysis-and-hemodiafiltration
#14
Kenji Tsuchida, Kojiro Nagai, Narushi Yokota, Satoru Yamada, Hiroyuki Michiwaki, Jun Minakuchi
BACKGROUND: With the identification of β2-microglobulin (β2MG) as an active participant in dialysis-related amyloid fibril formation, low-molecular-weight proteins (LMWPs) are now recognized as a distinct class of uremic toxins, and numerous compounds in this category have been identified. The class of LMWPs, although not precisely defined, has a molecular weight range of approximately 1,000-50,000 Da. With this in mind, dialysis prescriptions have been modified to increase the efficiency of uremic solute removal...
2017: Contributions to Nephrology
https://www.readbyqxmd.com/read/27951557/prospects-for-vascular-access-education-in-developing-countries-current-situation-in-cambodia
#15
Toshihide Naganuma, Yoshiaki Takemoto
We report our activities training doctors on vascular access procedures at International University (IU) Hospital in Cambodia through a program facilitated by Ubiquitous Blood Purification International, a nonprofit organization that provides medical support to developing countries in the field of dialysis medicine. Six doctors from Japan have been involved in the education of medical personnel at IU, and we have collectively visited Cambodia about 15 times from 2010 to 2016. In these visits, we have performed many operations, including 42 for arteriovenous fistula, 1 arteriovenous graft, and 1 percutaneous transluminal angioplasty...
2017: Contributions to Nephrology
https://www.readbyqxmd.com/read/27942355/role-of-toll-like-receptors-in-tuberculosis-infection
#16
Oguz Oben Biyikli, Aysegul Baysak, Gulfem Ece, Adnan Tolga Oz, Mustafa Hikmet Ozhan, Afig Berdeli
BACKGROUND: One-third of the world's population is infected with Mycobacterium tuberculosis. Investigation of Toll-like receptors (TLRs) has revealed new information regarding the immunopathogenesis of this disease. Toll-like receptors can recognize various ligands with a lipoprotein structure in the bacilli. Toll-like receptor 2 and TLR-4 have been identified in association with tuberculosis infection. OBJECTIVES: The aim of our study was to investigate the relationship between TLR polymorphism and infection progress...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27941495/evaluation-of-2-purification-methods-for-isolation-of-human-adipose-derived-stem-cells-based-on-red-blood-cell-lysis-with-ammonium-chloride-and-hypotonic-sodium-chloride-solution
#17
Sheng-Hong Li, Xuan Liao, Tian-En Zhou, Li-Ling Xiao, Yuan-Wen Chen, Fan Wu, Jing-Ru Wang, Biao Cheng, Jian-Xing Song, Hong-Wei Liu
BACKGROUND: The present study was conducted to compare 2 purification methods for isolation of human adipose-derived stromal vascular fraction or stem cells (ADSCs) based on red blood cell (RBC) lysis with 155 mM ammonium chloride (NH4Cl) and hypotonic sodium chloride (NaCl) solution, and try to develop a safe, convenient, and cost-effective purification method for clinical applications. METHODS: Adipose-derived stem cells and RBC were harvested from the fatty and fluid portions of liposuction aspirates, respectively...
January 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/27936718/heparin-like-chitosan-hydrogels-with-tunable-swelling-behavior-prolonged-clotting-times-and-prevented-contact-activation-and-complement-activation
#18
Xuelian Huang, Rui Wang, Ting Lu, Dongxu Zhou, Weifeng Zhao, Shudong Sun, Changsheng Zhao
The aim of this study was to create heparin-like chitosan hydrogels (HLCHs) for blood purification. Herein, we prepared two heparin-like chitosans (HLCSs) with various carboxymethyl and sulfate groups, followed by a cross-linking reaction with glutaraldehyde. The synthetic chitosan derivatives were characterized by X-ray photoelectron spectroscopy, gel permeation chromatography, FTIR and NMR. The average sulfonation degrees of two HLCSs were 0.69 and 0.94 per sugar unit, respectively. The swelling ratio of the HLCH could reach up to 4800%, and the HLCHs remained a well-defined shape and stable below 170 °C...
December 12, 2016: Biomacromolecules
https://www.readbyqxmd.com/read/27923319/non-invasive-swansea-criteria-are-valuable-alternatives-for-diagnosing-acute-fatty-liver-of-pregnancy-in-a-chinese-population
#19
Shan Wang, Shan-Ling Li, Yan-Xia Cao, Yan-Ping Li, Jin-Lai Meng, Xie-Tong Wang
BACKGROUND: This study aims to assess the diagnostic and prognostic value of Swansea criteria in diagnosing acute fatty liver of pregnancy (AFLP) in a Chinese population. METHODS: A retrospective study was conducted on 52 Chinese women diagnosed with AFLP. All selected cases were reassessed using the Swansea criteria with special focus on the non-invasive criteria, since performing a liver biopsy for this indication is rare in a Chinese population. RESULTS: Ninety point four percent of patients fulfilled five or more of the Swansea criteria...
December 6, 2016: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/27916705/newborn-screening-for-severe-combined-immunodeficiency-using-a-novel-and-simplified-method-to-measure-t-cell-excision-circles-trec
#20
Laura Tagliaferri, Joachim B Kunz, Margit Happich, Susanna Esposito, Thomas Bruckner, Daniel Hübschmann, Jürgen G Okun, Georg F Hoffmann, Ansgar Schulz, Judit Kappe, Carsten Speckmann, Martina U Muckenthaler, Andreas E Kulozik
The prognosis of children with severe combined immunodeficiency (SCID) depends on a presymptomatic diagnosis and early treatment before complications occur. We established and tested a simplified, practical and economic newborn screening method based on the quantification of T-cell receptor excision circles (TRECs) on dried blood spots (DBSs) through qPCR. Our method was validated by the analysis of 11 positive controls, which all showed an absence of TRECs, thus yielding a sensitivity of 100%. Further, we analyzed 6034 anonymized newborns of whom 6031 (99,95%) showed a normal TREC qPCR with a median of 600 estimated TREC copies/1...
December 2, 2016: Clinical Immunology: the Official Journal of the Clinical Immunology Society
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