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Blood purification

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https://www.readbyqxmd.com/read/29458095/rapid-loop-mediated-isothermal-amplification-detection-of-coeliac-disease-risk-alleles
#1
Michael Erlichster, Jason A Tye-Din, Michael D Varney, Efstratios Skafidas, Patrick Kwan
Human leukocyte antigen (HLA) genotyping has become a useful investigation in the diagnostic work-up of coeliac disease (CD), with utility in risk stratification and screening. However, broad application of this technology has been hindered by the cost and time burden of conventional laboratory-based assays. We have developed and validated CD-loop-mediated isothermal amplification (CD-LAMP), a LAMP assay, which enables rapid identification of the signature CD risk genotypes, HLA-DQ2.5, HLA-DQ8, HLA-DQ2.2, and HLA-DQA1*05...
February 16, 2018: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/29457280/continuous-blood-purification-ameliorates-clinical-signs-and-corrects-the-plasma-phospholipid-levels-of-patients-with-multiple-organ-dysfunction-syndromes
#2
Wei Wen, Kun Wang, Zhi-Ming Jiang, Zhong-Hui Zhang, Lei Zhou
BACKGROUND: Multiple organ dysfunction syndromes (MODS) is reported as a leading cause of mortality in intensive care units. Recently, continuous blood purification (CBP) has been mostly applied for MODS treatment. Thus, the purpose of this study was to investigate the effects of CBP on plasma phospholipid level in patients with MODS. METHODS: A total of 126 patients with MODS and 120 healthy people were collected. The serum cytokine levels, blood biochemical parameters, and blood gas indexes were detected, and the correlation among phospholipid compounds with serum cytokine levels, blood biochemical parameters, and blood gas indexes was analyzed...
February 18, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29445800/acoustic-separation-in-plastic-microfluidics-for-rapid-detection-of-bacteria-in-blood-using-engineered-bacteriophage
#3
P Dow, K Kotz, S Gruszka, J Holder, J Fiering
A more effective treatment of bacteremia requires a diagnostic platform that is both sensitive, accurate and rapid. Currently, clinical laboratory techniques require growth of bacteria prior to diagnosis, take days to complete, and leave empiric therapy and broad spectrum antibiotics as the only option at the onset of treatment. In order to bypass this growth requirement, we engineered a system that purifies bacteria from blood to improve performance in a bacteriophage-based luminescence assay. To perform the purification, we used acoustophoresis in plastic microfluidic chips, enabling future development into a low cost point-of-care system...
February 15, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29442909/synthetic-polysaccharides-as-drug-carriers-synthesis-of-polyglucose-amphotericin-b-conjugates-and-in-vitro-evaluation-of-their-anti-fungal-and-anti-leishmanial-activities
#4
Vasanthan Ravichandran, Gayathri P Kothandaraman, Christian Bories, Philippe M Loiseau, A Jayakrishnan
While many naturally occurring polysaccharides have been widely used as drug carriers, there are two main drawbacks in their use: the first is their physical properties such as molecular weight, branching, type of glycosidic linkages and solubility depend on their source and the method of isolation and purification, the second is many of them are contaminated with proteins and protein removal is essential for preventing immune reactions. Synthetic polysaccharides on the other hand can be tailor made from their respective monomers with consistent physical properties and are, free from protein contamination, both being significant advantages in their use...
April 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29429515/mice-dental-pulp-and-periodontal-ligament-endothelial-cells-exhibit-different-proangiogenic-properties
#5
Mohammad Ali Saghiri, Armen Asatourian, Christine M Sorenson, Nader Sheibani
Dental pulp is a highly vascularized tissue with a high regenerative capacity. This is attributed to its unique blood supply and the presence of progenitor or postnatal dental pulp stem cells. Here we aimed to isolate and compare the angiogenic properties of endothelial cells (EC) prepared from mouse dental pulp and periodontal ligament (PDL). EC were isolated from 4-week-old wild type immorto mice. Mice were sacrificed and after mandible isolation, the molar and incisor teeth and the PDL from molar teeth were dissected...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29429201/-survey-on-the-prevalence-of-continuous-blood-purification-in-chinese-pediatric-critical-care
#6
X Yang, S Y Qian, Y M Zhu, X Xu, C F Liu, F Xu, X X Ren, Y Wang, Y C Zhang, G P Lu
Objective: To investigate the current application status of continuous blood purification (CBP) technology and equipment in pediatric intensive care unit (PICU) in China. Methods: A cross-sectional survey was conducted to understand the current popularization of CBP technology and equipment, the management of CBP equipment and consumables, and the application of CBP in different diseases. A questionnaire named Application Status of Continuous Blood Purification Technology was applied. Children's hospitals and polyclinic hospitals with the pediatric qualification (pediatric emergency or critical care unit members of Chinese Medical Association and Chinese Medical Doctor Association) were selected...
February 2, 2018: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/29425448/design-of-carrageenan-based-heparin-mimetic-gel-beads-as-self-anticoagulant-hemoperfusion-adsorbents
#7
Xin Song, Kang Wang, Cheng-Qiang Tang, Wen-Wen Yang, Weifeng Zhao, Changsheng Zhao
The currently used hemoperfusion adsorbents such as activated carbon and ion exchange resin show dissatisfactory hemocompatibility, and a large dose of injected heparin leads to the increasing cost and the risk of systematic bleeding. Natural polysaccharide adsorbents commonly have good biocompatibility, but their application is restricted by the poor mechanical strength and low content of functional groups. Herein, we developed an efficient, self-anticoagulant and blood compatible hemoperfusion adsorbent by imitating the structure and functional groups of heparin...
February 9, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29423386/erythrocyte-membrane-unsaturated-mono-and-poly-fatty-acids-profile-in-newly-diagnosed-basal-cell-carcinoma-patients
#8
Fatemeh Rahrovani, Mohammad Hassan Javanbakht, Ehsan Ghaedi, Hamed Mohammadi, Amir-Hooshang Ehsani, Ali Esrafili, Mahmoud Djalali
Studies have reported different changes in the fatty acid composition of red blood cell (RBC) total lipids in patients with various types of cancer. It has been indicated that n-3/n-6 ratio plays a key role in the general consequence of skin photocarcinogenesis. However, to our knowledge there was no study examining the unsaturated fatty acid profile in basal cell carcinoma (BCC) patients. So, we explore the fatty acid composition of RBCs in newly diagnosed BCC patients in a hospital-based case-control study...
January 2018: Clinical Nutrition Research
https://www.readbyqxmd.com/read/29417731/super-high-flux-continuous-venovenous-hemodialysis-using-regional-citrate-anticoagulation-long-term-stability-of-middle-molecule-clearance
#9
Martin Siebeck, Thomas Dimski, Timo Brandenburger, Torsten Slowinski, Detlef Kindgen-Milles
Continuous renal replacement therapy is a standard treatment in critically ill patients with acute kidney injury. All CRRT techniques provide a high low-molecular weight clearance but even with hemofiltration, clearance of middle molecules is low. We investigated whether a new super high-flux hemofilter provides effective and sustained middle molecule clearance during citrate-anticoagulated continuous venovenous hemodialysis for up to 72 h. We included 14 critically ill patients with AKI-KDIGO-III in a prospective observational trial...
February 8, 2018: Therapeutic Apheresis and Dialysis
https://www.readbyqxmd.com/read/29413611/rationally-designed-magnetic-nanoparticles-as-anticoagulants-for-blood-purification
#10
Weifeng Zhao, Qiang Liu, Xiang Zhang, Baihai Su, Changsheng Zhao
Heparin-based anticoagulant drugs are widely used for the prevention of blood clotting during extracorporeal circuit (bloodlines or cassette system) and surgical procedures as well as for the treatment of thromboembolic events. However, these anticoagulants are associated with bleeding risks that demand continuous monitoring and neutralization with antidotes. We explore the possibility of utilizing anticoagulants for blood clotting prevention, then removing them before transfusing the blood back to body, thus avoid bleeding risks...
February 2, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29409663/penta-therapy-for-severe-acute-hyperlipidemic-pancreatitis-a-retrospective-chart-review-over-a-10-year-period
#11
Jian Lu, Yun Xie, Jiang Du, Mei Kang, Wei Jin, Yan Li, Hui Xie, Ruijie Cheng, Rui Tian, Ruilan Wang
INTRODUCTION: The purpose of our study is to evaluate the efficacy of penta-therapy for HL-SAP in a retrospective study. METHODS: Retrospective study between January 2007 and December 2016 in a hospital intensive care unit. HL-SAP patients were assigned to conventional treatment alone (the control group) or conventional treatment with the experimental protocol (the penta-therapy group) consists of blood purification, antihyperlipidemic agents, low-molecular weight heparin, insulin, covering the whole abdomen with Pixiao (a traditional Chinese medicine)...
February 3, 2018: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29409517/isolation-and-purification-of-glycosylphosphatidylinositols-gpis-in-the-schizont-stage-of-theileria-annulata-and-determination-of-antibody-response-to-gpi-anchors-in-vaccinated-and-infected-animals
#12
Toktam Abbasnia, Ahmad Asoodeh, Gholamreza Habibi, Alireza Haghparast
BACKGROUND: Tropical theileriosis is widely distributed from North Africa to East Asia. It is a tick-borne disease caused by Theileria annulata, an obligate two-host intracellular protozoan parasite of cattle. Theileria annulata use leukocytes and red blood cells for completion of the life-cycle in mammalian hosts. The stage of Theileria annulata in monocytes and B lymphocytes of cattle is an important step in pathogenicity and diagnosis of the disease. Glycosylphosphatidylinositols (GPIs) are a distinct class of glycolipid structures found in eukaryotic cells and are implicated in several biological functions...
February 6, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29404509/newly-discovered-hepatitis-c-virus-minicores-circulate-in-human-blood
#13
Francis J Eng, Ahmed El-Shamy, Erin H Doyle, Arielle Klepper, A Scott Muerhoff, Andrea D Branch
Hepatitis C virus (HCV) is one of the most prevalent causes of chronic blood-borne infections worldwide. Despite developments of highly effective treatments, most infected individuals are unaware of their infection. Approximately 75% of infections are in low- and middle-income countries; therefore, continuing research in HCV molecular virology and the development of vaccines and affordable diagnostics is required to reduce the global burden. Various intracellular forms of the HCV nucleocapsid (core) protein are produced in cell culture; these comprise the conventional p21 core and the newly discovered shorter isoforms (minicores)...
January 2018: Hepatology Communications
https://www.readbyqxmd.com/read/29404413/bioid-reveals-novel-proteins-of-the-plasmodium-parasitophorous-vacuole-membrane
#14
Cilly Bernardette Schnider, Damaris Bausch-Fluck, Francis Brühlmann, Volker T Heussler, Paul-Christian Burda
During their development within the vertebrate host, Plasmodium parasites infect hepatocytes and red blood cells. Within these cells, parasites are surrounded by a parasitophorous vacuole membrane (PVM). The PVM plays an essential role for the interaction of parasites with their host cells; however, only a limited number of proteins of this membrane have been identified so far. This is partially because systematic proteomic analysis of the protein content of the PVM has been difficult in the past, due to difficulties encountered in attempts to separate the PVM from other membranes such as the parasite plasma membrane...
January 2018: MSphere
https://www.readbyqxmd.com/read/29390237/-customization-of-hemodialysis-therapy-dialysis-is-not-a-washing-machine
#15
EDITORIAL
Antonio Santoro
In recent years, the population of chronic dialysis has grown in number but also in age and frequency of co-morbidies such as cardiac diseases, vascular pathologies, diabetes, etc. The majority of patients on chronic hemodialysis are over 70 years and, given the high number of comorbidities, they often exhibit poor tolerance to dialysis treatments. A non-tolerated dialytic treatment can have side-effects that would require an intensification of the dialysis sessions and many hospitalizations. Consequently, the problematic dialysis treatments, as well as harmful for the patient, become economically more detrimental than other treatments apparently more expensive but more tolerated ones In the current days we have, thanks to the huge developments in dialysis technology, powerful weapons to ensure effective and scarcely symptomatic dialysis treatments to the majority of the HD patients...
February 2018: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
https://www.readbyqxmd.com/read/29389948/photocleavage-based-affinity-purification-of-biomarkers-from-serum-application-to-multiplex-allergy-testing
#16
Zhi Wan, Heather P Ostendorff, Ziying Liu, Lynda C Schneider, Kenneth J Rothschild, Mark J Lim
Multiplex serological immunoassays, such as implemented on microarray or microsphere-based platforms, provide greater information content and higher throughput, while lowering the cost and blood volume required. These features are particularly attractive in pediatric food allergy testing to facilitate high throughput multi-allergen analysis from finger- or heel-stick collected blood. However, the miniaturization and microfluidics necessary for creating multiplex assays make them highly susceptible to the "matrix effect" caused by interference from non-target agents in serum and other biofluids...
2018: PloS One
https://www.readbyqxmd.com/read/29375123/the-chemokine-mcp-1-ccl2-in-the-host-interaction-with-cancer-a-foe-or-ally
#17
REVIEW
Teizo Yoshimura
Macrophages are one of the most abundant leukocyte populations infiltrating tumor tissues and can exhibit both tumoricidal and tumor-promoting activities. In 1989, we reported the purification of monocyte chemoattractant protein-1 (MCP-1) from culture supernatants of mitogen-activated peripheral blood mononuclear cells and tumor cells. MCP-1 is a potent monocyte-attracting chemokine, identical to the previously described lymphocyte-derived chemotactic factor or tumor-derived chemotactic factor, and greatly contributes to the recruitment of blood monocytes into sites of inflammatory responses and tumors...
January 29, 2018: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/29373818/a-novel-fibrinolytic-serine-metalloprotease-from-the-marine-serratia-marcescens-subsp-sakuensis-purification-and-characterization
#18
Anusha Krishnamurthy, Prasanna Devarbhat Belur
This study demonstrates the purification and characterization of a fibrinolytic serine metalloprotease from the marine Serratia marcescens subsp. sakuensis (KU296189.1). The purified enzyme (1033 U/mg) had a molecular weight of 43 KDa, with optimum pH and temperature being 7 and 55 °C. The in vitro half-life of the fibrinolytic enzyme at 37 °C was found to be 19 h. The kinetic constants, Km and Vmax of the purified enzyme determined using fibrin as substrate was 0.66 mg/mL and 158.73 U/mL. The Kcat and catalytic efficiency of the enzyme was found to be 12...
January 23, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29373719/quantification-of-15-psychotropic-drugs-in-serum-and-postmortem-blood-samples-after-a-modified-mini-quechers-by-uhplc-ms-ms
#19
A Pouliopoulos, E Tsakelidou, A Krokos, H G Gika, G Theodoridis, N Raikos
The aim of the study was to develop a LC-MS-MS method able to detect and quantify a number of frequently prescribed antipsychotic and antidepressant drugs for toxicological purposes. Separation of compounds was performed on a C-18 RP column by Ultra High-Pressure Chromatography over a 11 min run. A modified single step QuEChERS protocol consisted essentially by the addition of acetonitrile, potassium carbonate and magnesium sulfate in 100 μL of sample, vortexing, centrifugation and evaporation has been selected...
January 24, 2018: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/29371118/ankrd1-regulates-innate-immune-responses-against-herpes-simplex-virus-1-a-potential-role-in-eczema-herpeticum
#20
Lianghua Bin, Xiaozhao Li, Brittany Richers, Joanne E Streib, Jack W Hu, Patricia Taylor, Donald Y M Leung
BACKGROUND: Atopic dermatitis (AD) is a common inflammatory skin disease. A subset of AD is susceptible to disseminated herpes simplex virus (HSV) infection, a complication termed as eczema herpeticum(ADEH+). The immune mechanisms causing ADEH+ remain elusive. Using RNA-sequencing, we recently found that ankyrin repeat domain 1 (ANKRD1) was significantly induced in human peripheral blood mononuclear cells (PBMCs) upon HSV-1 stimulation; and its induction in ADEH+ was significantly reduced as compared to AD patients without a history of EH (ADEH-)...
January 22, 2018: Journal of Allergy and Clinical Immunology
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