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Blood purification

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https://www.readbyqxmd.com/read/29243277/purification-of-angiotensin-converting-enzyme-from-human-plasma-and-investigation-of-the-effect-of-some-active-ingredients-isolated-from-nigella-sativa-l-extract-on-the-enzyme-activity
#1
Zehra Basi, Vedat Turkoglu
In the present study, one-step purification of angiotensin converting enzyme (ACE, peptidyldipeptidase A, EC 3.4.15.1), responsible from regulation of blood pressure, was achieved using affinity chromatography from human plasma. The enzyme was purified 12860-fold with a spesific activtiy of 5080 EU/mg.protein. Optimum temperature and pH were determined for the enzyme as 35-40 °C and pH 7.4-7.5, respectively. The purity of ACE was determined by SDS-PAGE and the enzyme showed two bands at 60 kDa and 70 kDa on the gel...
December 15, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29234445/bioactivity-guided-fractionation-of-the-traditional-chinese-medicine-resina-draconis-reveals-loureirin-b-as-a-pai-1-inhibitor
#2
Yu Jiang, Guangping Zhang, Dong Yan, Hong Yang, Zuguang Ye, Tonghui Ma
Thrombotic diseases have become a global burden due to morbidity, mortality, and disability. Traditional Chinese medicine has been proven effective in removing blood stasis and promoting blood circulation, but the exact mechanisms remain unclear. Plasminogen activator inhibitor-1 (PAI-1) is a natural inhibitor of tissue-type and urokinase-type plasminogen activators. In this study, we screened four fractions of Resina Draconis (a traditional Chinese medicine) extract for PAI-1 inhibitory activity. Bioactivity-guided purification and chromogenic substrate-based assay led to the identification of loureirin B as the major PAI-1 inhibitor, with an IC50 value of 26...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29234179/vitamin-e-coated-polysulfone-membrane-based-hemodiafiltration-attenuates-inflammation-in-a-rat-model-of-lipopolysaccharide-induced-systemic-inflammation
#3
Yoshihiro Hatanaka, Satoru Inoue, Koji Goto, Norihisa Yasuda, Seigo Hidaka, Takaaki Kitano
Background: Acute blood purification (ABP) therapy is used regularly in the clinical setting and reportedly alleviates organ failure associated with severe systemic inflammatory responses, leading to reduced mortality. The present study aimed to determine whether there is a difference in efficacy between polysulfone (PS) membranes, which are currently used regularly in the clinical setting, and vitamin E-coated polysulfone (VEPS) membranes, which are anticipated to exhibit the antioxidant and anti-inflammatory properties of vitamin E...
September 2017: Iranian Journal of Medical Sciences
https://www.readbyqxmd.com/read/29234006/enhancing-size-based-size-separation-through-vertical-focus-microfluidics-using-secondary-flow-in-a-ridged-microchannel
#4
Bushra Tasadduq, Wilbur Lam, Alexander Alexeev, A Fatih Sarioglu, Todd Sulchek
High throughput size based separation and sorting of bioparticles and cells is critical to a variety of biomedical processing steps for medical diagnostics and pharmaceutical purification. Improving microfluidic size-based particle/cell sorting is a challenge to better address the need for generating more homogeneous subpopulations for study and use. We propose a novel advance to microfluidic sorting devices that uses three-dimensional focusing of the sample to optimally position particles to amplify the size-dependent differences in trajectories caused by differential secondary flows...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29231971/enzooty-of-non-hodgkin-s-malignant-lymphoma-of-papio-hamadryas-in-sukhumi-monkey-colony-clinical-and-morphological-signs-of-pre-lymphoma
#5
Lelita A Yakovleva, Boris A Lapin, Aslan A Agumava
Inoculation of hamadryas baboons with blood of leukemia ill people-induced malignant non-Hodgkin's lymphoma in experimental animals for a very considerable latency period. At close contact of inoculated baboons with healthy non-inoculated animals, the lymphoma spread between them. The epidemiological analysis, postmortem examination, histological analysis, tissue culturing, and PCR were used for the diagnostics of lymphoma and pre-lymphoma, purification, identification of STLV-1, and HVP viruses. Characteristic clinical and morphological signs designated by us as pre-lymphoma often precede the lymphoma development...
December 12, 2017: Journal of Medical Primatology
https://www.readbyqxmd.com/read/29231025/-comparison-of-mpure-12-automatic-nucleic-acid-purification-and-chelex-100-method
#6
X Shen, M Li, Y L Wang, Y L Chen, Y Lin, Z M Zhao, T Z Que
OBJECTIVES: To explore the forensic application value of MPure-12 automatic nucleic acid purification (MPure-12 Method) for DNA extraction by extracting and typing DNA from bloodstains and various kinds of biological samples with different DNA contents. METHODS: Nine types of biological samples, such as bloodstains, semen stains, and saliva were collected. DNA were extracted using MPure-12 method and Chelex-100 method, followed by PCR amplification and electrophoresis for obtaining STR-profiles...
April 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29224166/dna-methylation-analysis-from-blood-spots-increasing-yield-and-quality-for-genome-wide-and-locus-specific-methylation-analysis
#7
Akram Ghantous, Hector Hernandez-Vargas, Zdenko Herceg
Blood represents an easily accessible human tissue for numerous research and clinical applications, including surrogate roles for biomarkers of other tissues. Dried blood spots (DBS) are space- and cost-efficient storage forms of blood while stably retaining many of its chemical constituents. Consequently, neonatal DBS are routinely collected in many countries, and their biobanks represent gold mines for research. However, the utility of DBS is restricted by the limited amount and quality of extractable biomolecules (including DNA), especially for genome-wide profiling...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29214671/multilayer-cucurbit-6-uril-based-magnetic-nanoparticles-prepared-via-host-guest-interaction-remarkable-adsorbent-for-the-removal-of-low-density-lipoprotein-from-plasma
#8
Dandan Jiang, Xiqian Li, Qiong Jia
Efficient removal of low density lipoprotein (LDL) is a key challenge since its high level in plasma is a primary risk factor in the pathogenesis of atherosclerotic cardiovascular disease. In this work, a facile synthesis strategy based on host-guest interactions was developed to prepare multilayer cucurbit[6]uril-based magnetic nanoparticles, MNPs-(HA-DAH5/HA-CB[6]5). The compound was employed as a blood purification material for the removal of LDL from plasma because it had good blood compatibilitiy and could be easily separated with an external magnet...
December 6, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29211557/acoustophoretic-purification-of-platelets-feasibility-and-impact-on-platelet-activation-and-function
#9
Pierre Bohec, Jérémie Gachelin, Véronique Ollivier, Thibaut Mutin, Xavier Télot, Benoît Ho-Tin-Noé, Sandra Sanfilippo
Purity, limited platelet activation, and preservation of platelet function are important stakes of preparation of platelet concentrates (PC) for clinical use. In fact, contaminating red blood cells and leukocytes, as well as activated and/or poorly functional platelets in PC, represents a risk of poor efficiency and adverse side effects during platelet transfusion. Therefore, optimization of preparation and storage of PC is still an active field of research. Shear-induced platelet activation is an unwanted side effect of the hard-spin (up to 5000g) step of centrifugation-based methods currently used in blood banks to prepare PC from whole blood samples...
December 6, 2017: Platelets
https://www.readbyqxmd.com/read/29207229/-bilirubin-removal-with-coupled-plasma-filtration-and-adsorption-in-patients-affected-by-hilar-cholangiocarcinoma
#10
Walter De Simone, Francesco Crafa, Adele Noviello, Francesco Esposito, Bruno Zito, Rocco Manganelli, Antonella De Simone, Luigi Covotta, Giuseppe Palladino, Emanuele De Simone
Background: Patients affected by hilar cholangiocarcinoma are eligible for surgery only in the 20-30% of the cases and postoperative mortality is 40-50%. Many specialists are involved in the treatment of this disease, like surgeons, gastroenterologists, oncologists and radiotherapists. Recent studies have shown that preoperative bilirubinaemia is a predictor of morbidity and mortality after surgery. Coupled Plasma Filtration and Adsorption (CPFA) is a blood purification extracorporeal therapy recommended for sepsis and able to reduce bilirubinaemia...
December 5, 2017: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
https://www.readbyqxmd.com/read/29202360/enhanced-capillary-electrophoretic-screening-of-alzheimer-based-on-direct-apolipoprotein-e-genotyping-and-one-step-multiplex-pcr
#11
Nain Woo, Su-Kang Kim, Yucheng Sun, Seong Ho Kang
Human apolipoprotein E (ApoE) is associated with high cholesterol levels, coronary artery disease, and especially Alzheimer's disease. In this study, we developed an ApoE genotyping and one-step multiplex polymerase chain reaction (PCR) based-capillary electrophoresis (CE) method for the enhanced diagnosis of Alzheimer's. The primer mixture of ApoE genes enabled the performance of direct one-step multiplex PCR from whole blood without DNA purification. The combination of direct ApoE genotyping and one-step multiplex PCR minimized the risk of DNA loss or contamination due to the process of DNA purification...
November 27, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29195219/nanoconjugates-of-ferrocene-and-carbon-encapsulated-iron-nanoparticles-as-sensing-platforms-for-voltammetric-determination-of-ceruloplasmin-in-blood
#12
Jakub P Sęk, Artur Kasprzak, Michał Bystrzejewski, Magdalena Poplawska, Waldemar Kaszuwara, Zbigniew Stojek, Anna M Nowicka
The nanoparticles comprising of iron core and carbon shell were decorated with ferrocene derivatives: ferrocenecarboxaldehyde (Fc-1) and ferrocenecarboxaldehyde oxime (Fc-2). A microdrop of suspension of the nanoconjugate was placed on a glassy-carbon electrode to prepare the recognition/sensing layer. Drying and purification of the sensing layer resulted in a well-defined and stable square-wave voltammogram of the ferrocene moiety. The height of the voltammetric peak increased in the presence of ceruloplasmin...
November 23, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29187662/-primary-culture-of-human-normal-epithelial-cells
#13
Yu Tang, Wenji Xu, Wanbei Guo, Ming Xie, Huilong Fang, Chen Chen, Jun Zhou
The traditional primary culture methods of human normal epithelial cells have disadvantages of low activity of cultured cells, the low cultivated rate and complicated operation. To solve these problems, researchers made many studies on culture process of human normal primary epithelial cell. In this paper, we mainly introduce some methods used in separation and purification of human normal epithelial cells, such as tissue separation method, enzyme digestion separation method, mechanical brushing method, red blood cell lysis method, percoll layered medium density gradient separation method...
November 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/29186770/the-evaluation-of-pro-cognitive-and-antiamnestic-properties-of-berberine-and-magnoflorine-isolated-from-barberry-species-by-centrifugal-partition-chromatography-cpc-in-relation-to-qsar-modelling
#14
(no author information available yet)
Civilization diseases associated with memory disorders are important health problems occurring due to a prolonged life span. The manuscript shows the results of an in vivo study targeting the emergence of two drug candidates with anti-amnestic properties. The preceding quantitative structure-activity relationship (QSAR) studies provided information on the ability of berberine and magnoflorine to cross the blood-brain barrier (BBB). In the light of these findings, both compounds were purified from crude plant extracts of barberries: berberine-from Berberis siberica using a method published earlier, and magnoflorine-from Berberis cretica by centrifugal partition chromatography (solvent system: ethyl acetate:butanol:water-0...
November 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29185591/safety-and-efficacy-of-regional-citrate-anticoagulation-in-continuous-blood-purification-treatment-of-patients-with-multiple-organ-dysfunction-syndrome
#15
B Tuerdi, L Zuo, H Sun, K Wang, Z Wang, G Li
The aim of this study was to discuss the safety and efficacy of regional citrate anticoagulation (RCA) on continuous blood purification (CBP) during the treatment of multiple organ dysfunction syndrome (MODS). Thirty-five patients with MODS were divided into two groups: the local citrate anticoagulation (RCA) group, and the heparin-free blood purification (hfBP) group. The MODS severity was assessed according to Marshall's MODS score criteria. Blood coagulation indicators, blood pressure, filter lifespan, filter replacement frequency, anticoagulation indicators, and main metabolic and electrolyte indicators were analyzed and compared between RCA and hfBP groups...
November 17, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/29178943/high-volume-hemofiltration-in-adult-burn-patients-with-septic-shock-and-acute-kidney-injury-a-multicenter-randomized-controlled-trial
#16
Kevin K Chung, Elsa C Coates, David J Smith, Rachel A Karlnoski, William L Hickerson, Angela L Arnold-Ross, Michael J Mosier, Marcia Halerz, Amy M Sprague, Robert F Mullins, Daniel M Caruso, Marlene Albrecht, Brett D Arnoldo, Agnes M Burris, Sandra L Taylor, Steven E Wolf
BACKGROUND: Sepsis and septic shock occur commonly in severe burns. Acute kidney injury (AKI) is also common and often results as a consequence of sepsis. Mortality is unacceptably high in burn patients who develop AKI requiring renal replacement therapy and is presumed to be even higher when combined with septic shock. We hypothesized that high-volume hemofiltration (HVHF) as a blood purification technique would be beneficial in this population. METHODS: We conducted a multicenter, prospective, randomized, controlled clinical trial to evaluate the impact of HVHF on the hemodynamic profile of burn patients with septic shock and AKI involving seven burn centers in the United States...
November 25, 2017: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/29169803/expression-purification-and-characterization-of-two-leucine-aminopeptidases-of-the-blood-fluke-schistosoma-mansoni
#17
Gabriela Maggioli, Gabriel Rinaldi, Ines Giaudrone, Patricia Berasain, José F Tort, Paul J Brindley, Carlos Carmona
Schistosomiasis is a major neglected tropical disease (NTD) and considered the most important of the human helminthiases in terms of morbidity and mortality. Whereas treatment with praziquantel has been effective since the 1980s, the potential for the emergence of drug resistance has propelled the search for new interventions. Studies have revealed key roles of proteases in parasitic helminths during establishment of infection, tissue invasion, immune evasion, parasite feeding and development throughout the different developmental stages, pinpointing them as possible candidates...
November 20, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29168915/a-novel-microdevice-for-rapid-neutrophil-purification-and-phenotyping-in-type-2-diabetes-mellitus
#18
Hui Min Tay, Rinkoo Dalan, King Ho Holden Li, Bernhard O Boehm, Han Wei Hou
Neutrophil dysfunction is strongly linked to type 2 diabetes mellitus (T2DM) pathophysiology, but the prognostic potential of neutrophil biomarkers remains largely unexplored due to arduous leukocyte isolation methods. Herein, a novel integrated microdevice is reported for single-step neutrophil sorting and phenotyping (chemotaxis and formation of neutrophil extracellular traps (NETosis)) using small blood volumes (fingerprick). Untouched neutrophils are purified on-chip from whole blood directly using biomimetic cell margination and affinity-based capture, and are exposed to preloaded chemoattractant or NETosis stimulant to initiate chemotaxis or NETosis, respectively...
November 23, 2017: Small
https://www.readbyqxmd.com/read/29164154/the-application-of-dextran-sedimentation-as-an-initial-step-in-neutrophil-purification-promotes-their-stimulation-due-to-the-presence-of-monocytes
#19
Alex Quach, Antonio Ferrante
The purification of human neutrophils for in vitro studies is challenging as they are easily activated through ex vivo manipulations. The technique of erythrocyte sedimentation combined with density gradient centrifugation remains widely practiced and was the subject of this study. Since in the sedimentation step the leukocytes are incubated with dextran, we have raised the likelihood that cellular activation would occur with mediator release leading to neutrophil activation. By comparing the activity of neutrophils purified from whole blood by the classical 2-step method of dextran sedimentation followed by low-density Ficoll-Hypaque (1...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/29161268/nucleic-acid-purification-from-plants-animals-and-microbes-in-under-30-seconds
#20
Yiping Zou, Michael Glenn Mason, Yuling Wang, Eugene Wee, Conny Turni, Patrick J Blackall, Matt Trau, Jose Ramon Botella
Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples. To address this issue, we investigated a variety of materials for their suitability for nucleic acid capture and purification. We report here that untreated cellulose-based paper can rapidly capture nucleic acids within seconds and retain them during a single washing step, while contaminants present in complex biological samples are quickly removed...
November 2017: PLoS Biology
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