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Blood purification

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https://www.readbyqxmd.com/read/28543387/the-hematocrit-affects-the-volume-of-plasma-treated-with-coupled-plasma-filtration-and-adsorption-with-predilution
#1
Stefano Finazzi, Elena Garbero, Giampietro Trussardi, Guido Bertolini
Coupled plasma filtration and adsorption (CPFA) is an extracorporeal blood purification technique proposed for the treatment of septic-shock. By removing pro- and anti-inflammatory mediators from plasma, CPFA is supposed to have a therapeutic effect on the abnormal inflammatory response seen in this condition. Recently, blood predilution with citrate solution has been adopted to prevent clotting in the CPFA circuit-one of the main problems of the technique. Taking into account the patient's hematocrit, we worked out a formula for the volume of plasma effectively treated by CPFA after predilution...
May 2017: Artificial Organs
https://www.readbyqxmd.com/read/28536858/quantification-of-micrornas-directly-from-body-fluids-using-a-base-stacking-isothermal-amplification-method-in-a-point-of-care-device
#2
Maggie R Williams, Robert D Stedtfeld, Tiffany M Stedtfeld, James M Tiedje, Syed A Hashsham
MicroRNAs have been proposed to be a class of biomarkers of disease as expression levels are significantly altered in various tissues and body fluids when compared to healthy controls. As such, the detection and quantification of microRNAs is imperative. While many methods have been established for quantification of microRNAs, they typically rely on time consuming handling such as RNA extraction, purification, or ligation. Here we describe a novel method for quantification of microRNAs using direct amplification in body fluids without upstream sample preparation...
September 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28532053/functionalized-fe3o4-graphene-oxide-nanocomposites-with-hairpin-aptamers-for-the-separation-and-preconcentration-of-trace-pb-2-from-biological-samples-prior-to-determination-by-icp-ms
#3
Mojtaba Shamsipur, Leila Farzin, Mahmoud Amouzadeh Tabrizi, Shahab Sheibani
Lead (Pb) as a topically poisonous metal represents a serious threat to the ecological environment and especially to human beings. Therefore, it is urgent to develop a rapid and reliable monitoring technique for this heavy metal in the environmental samples. In the present study, we have designed a selective and sensitive method for the determination of ultratrace contents of Pb(2+) in biological samples, based on the guanine (G)-quadruplex formed by the aptamer with hairpin structure and Pb(2+). For this purpose, Pb(2+) specific aptamer serving as affinity probe to capture and separate trace amounts of the analyte, was covalently linked to Fe3O4/graphene oxide (GO) surface by using a suitable cross-linking agent...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28524034/-value-of-preoperative-pulmonary-artery-diastolic-pressure-on-predicting-primary-graft-dysfunction-after-bilateral-lung-transplantation-for-patients-with-idiopathic-pulmonary-fibrosis
#4
Feng Zhang, Hongyang Xu, Shuyun Jiang, Jiaqiong Li, Shunmei Lu, Dapeng Wang, Zhidong Zang, Hong Pan, Jingyu Chen
OBJECTIVE: To analyze the value of the potential risk factors on predicting primary graft dysfunction (PGD) after bilateral lung transplantation for the patients with idiopathic pulmonary fibrosis (IPF). METHODS: A retrospective study was conducted. Fifty-eight patients with IPF who underwent the bilateral lung transplantation admitted to Wuxi People's Hospital Affiliated to Nanjing Medical University from June 2014 to March 2017 were enrolled. The grade 3 PGD happened within 72 hours after transplantation was taken as the outcome event, and these patients were divided into PGD and non-PGD groups...
May 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28523846/a-novel-rapid-analysis-using-mass-spectrometry-to-evaluate-downstream-refolding-of-recombinant-human-insulin-like-growth-factor-1-mecasermin
#5
Kenichiro Furuki, Toshimasa Toyo'oka, Hideto Yamaguchi
RATIONALE: Mecasermin is used to treat elevated blood sugar as well as growth hormone-resistant Laron-type dwarfism. Mecasermin isolated from inclusion bodies in extracts of E.coli must be refolded to acquire sufficient activity. However, there is no rapid analytical method for monitoring refolding during the purification process. METHODS: We prepared mecasermin drug product, in-process samples during the oxidation of mecasermin, forced-reduced mecasermin, and aerially oxidized mecasermin after forced reduction...
May 19, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28522650/examining-the-association-between-hemodialysis-access-type-and-mortality-the-role-of-access-complications
#6
Pietro Ravani, Robert Quinn, Matthew Oliver, Bruce Robinson, Ronald Pisoni, Neesh Pannu, Jennifer MacRae, Braden Manns, Brenda Hemmelgarn, Matthew James, Marcello Tonelli, Brenda Gillespie
BACKGROUND AND OBJECTIVES: People receiving hemodialysis to treat kidney failure need a vascular access (a fistula, a graft, or a central venous catheter) to connect to the blood purification machine. Higher rates of access complications are considered the mechanism responsible for the excess mortality observed among catheter or graft users versus fistula users. We tested this hypothesis using mediation analysis. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We studied incident patients who started hemodialysis therapy from North America, Europe, and Australasia (the Dialysis Outcomes and Practice Patterns Study; 1996-2011)...
May 18, 2017: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/28514968/further-evaluation-of-the-nwf-filter-for-the-purification-of-plasmodium-vivax-infected-erythrocytes
#7
Jiangyan Li, Zhiyong Tao, Qian Li, Awtum Brashear, Ying Wang, Hui Xia, Qiang Fang, Liwang Cui
BACKGROUND: Isolation of Plasmodium-infected red blood cells (iRBCs) from clinical blood samples is often required for experiments, such as ex vivo drug assays, in vitro invasion assays and genome sequencing. Current methods for removing white blood cells (WBCs) from malaria-infected blood are time-consuming or costly. A prototype non-woven fabric (NWF) filter was developed for the purification of iRBCs, which showed great efficiency for removing WBCs in a pilot study. Previous work was performed with prototype filters optimized for processing 5-10 mL of blood...
May 17, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28510609/spr-based-fragment-screening-with-neurotensin-receptor-1-generates-novel-small-molecule-ligands
#8
Sylwia Huber, Fabio Casagrande, Melanie N Hug, Lisha Wang, Philipp Heine, Lutz Kummer, Andreas Plückthun, Michael Hennig
The neurotensin receptor 1 represents an important drug target involved in various diseases of the central nervous system. So far, the full exploitation of potential therapeutic activities has been compromised by the lack of compounds with favorable physicochemical and pharmacokinetic properties which efficiently penetrate the blood-brain barrier. Recent progress in the generation of stabilized variants of solubilized neurotensin receptor 1 and its subsequent purification and successful structure determination presents a solid starting point to apply the approach of fragment-based screening to extend the chemical space of known neurotensin receptor 1 ligands...
2017: PloS One
https://www.readbyqxmd.com/read/28510363/purification-toxoplasma-gondii-tissue-cysts-using-percoll-gradients
#9
Elizabeth A Watts, Animesh Dhara, Anthony P Sinai
The protozoan parasite Toxoplasma gondii is capable of infecting all warm-blooded animals and humans. Infectious, transmissible forms of the parasite include oocysts produced by the sexual cycle within the definitive feline host and tissue cysts that form Toxoplasma in the central nervous system and muscle during the asexual cycle within all chronically infected warm-blooded hosts. These tissue cysts are populated with slow-growing bradyzoites, which until recently have been thought to be dormant entities in the context of immune sufficiency...
May 16, 2017: Current Protocols in Microbiology
https://www.readbyqxmd.com/read/28502740/isolation-of-an-insulin-like-peptide-from-the-asian-malaria-mosquito-anopheles-stephensi-that-acts-as-a-steroidogenic-gonadotropin-across-diverse-mosquito-taxa
#10
Andrew B Nuss, Mark R Brown
Many insulin-like peptides (ILPs) have been identified in insects, yet only a few were isolated in their native form for structural and functional studies. Antiserum produced to ILP3 in Aedes aegypti was used in a radioimmunoassay to monitor the purification of an ILP from heads of adult An. stephensi and recognized the ILP in other immunoassays. The structure of the purified peptide matched that predicted for the ILP3 in this species. The native form stimulated ecdysteroid production by ovaries isolated from non-blood fed females...
May 11, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28501598/microgramma-vacciniifolia-polypodiaceae-fronds-contain-a-multifunctional-lectin-with-immunomodulatory-properties-on-human-cells
#11
Leydianne Leite de Siqueira Patriota, Thamara Figueiredo Procópio, Jéssica de Santana Brito, Virginie Sebag, Ana Patrícia Silva de Oliveira, Ana Karine de Araújo Soares, Leyllane Rafael Moreira, Thâmarah de Albuquerque Lima, Tatiana Soares, Túlio Diego da Silva, Patrícia Maria Guedes Paiva, Virgínia Maria Barros de Lorena, Cristiane Moutinho Lagos de Melo, Lidiane Pereira de Albuquerque, Thiago Henrique Napoleão
In this study, we report the purification and characterization of a multifunctional lectin (MvFL) from Microgramma vacciniifolia fronds as well as its immunomodulatory properties on human peripheral blood mononuclear cells (PBMCs). MvFL (pI 4.51; 54kDa) is a glycoprotein able to inhibit trypsin activity and that has sequence similarities (32% coverage) with a plant RNA-binding protein. Hemagglutinating activity of MvFL was not altered by heating at 100°C for 30min, but was reduced in alkaline pH (8.0 and 9...
May 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28485326/clarifications-on-continuous-renal-replacement-therapy-and-hemodynamics
#12
REVIEW
Xiao-Ting Wang, Cui Wang, Hong-Min Zhang, Da-Wei Liu
OBJECTIVE: Continuous renal replacement therapy (CRRT) is a continuous process of bedside blood purification which is widely used in the treatment of acute kidney injury (AKI) and for fluid management. However, since AKI and fluid overload are often found to be associated with hemodynamic abnormalities, determining the relationship between CRRT and hemodynamics remains a challenge in the treatment of critically ill patients. The aim of this review was to summarize key points in the relationship between CRRT and hemodynamics and to understand and monitor renal hemodynamics in critically ill patients, especially those with AKI...
May 20, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28485212/purification-and-characterization-of-hemolysin-from-periodontopathogenic-bacterium-eikenella-corrodens-strain-1073
#13
Fariha Jasin Mansur, Sari Takahara, Mihoko Yamamoto, Masafumi Shimatani, Mohammad Minnatul Karim, Yuichiro Noiri, Shigeyuki Ebisu, Hiroyuki Azakami
Eikenella corrodens 1073 was found to show hemolytic activity when grown on sheep blood agar. A high and dose-dependent hemolytic activity was detected in the cell envelope fraction, which was further purified by ion-exchange and gel-filtration chromatography. Consequently, a 65-kDa protein with hemolytic activity was obtained, suggesting that this protein might be a hemolysin. Its N-terminal amino acid sequence was nearly identical to that of X-prolyl aminopeptidase from E. corrodens ATCC 23834. To confirm that X-prolyl aminopeptidase functions as a hemolytic factor, we expressed the hlyA gene, encoding X-prolyl aminopeptidase, in Escherichia coli...
June 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28483384/-online-hemodiafiltration-practical-aspects-safety-and-efficacy
#14
Bernard Canaud, Leïla Chénine, Hélène Leray-Moraguès, Laure Patrier, Annie Rodriguez, A Gontier-Picard, Marion Moréna
Purification of high molecular uremic toxins by conventional hemodialysis is limited. It remains associated with a high morbidity and excessively high mortality. Online hemodiafiltration using a high permeability hemodiafilter, an ultrapure dialysate, and which tends to maximize substitution volumes, provides a high efficiency and low bio-incompatibility renal supplementation. Regular use of online hemodiafiltration is associated with reduced morbidity (reduction of intradialytic hypotension episodes, improved blood pressure control, reduced inflammatory profile, better anemia correction and prevention of β2-microglobulin-associated amyloidosis)...
May 2017: Néphrologie & Thérapeutique
https://www.readbyqxmd.com/read/28482383/-efficacy-of-continuous-blood-purification-in-treatment-of-severe-acute-pancreatitis-in-children
#15
Y Zhu, Y Cui, Y C Zhang, H J Miao, F Wang, R X Chen, Q F Rong
Objective: To explore the therapeutic role of bedside continuous blood purification(CBP) in children with severe acute pancreatitis(SAP). Method: The clinical and laboratory data of 11 children with SAP who were admitted to Pediatric Intensive Care Unit (PICU) of Shanghai Children's Hospital from June 2013 to May 2016 were analyzed, including using pediatric critical illness score (PCIS) and pediatric risk of score mortality (PRISM)-Ⅲ score to assessing the severity of the disease.For those patients with severe organ dysfunction, CBP treatment was used when conventional therapy was not efficient...
May 4, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/28481454/severe-acute-valproic-acid-intoxication-successfully-treated-with-liver-support-therapy
#16
Yongchun Ge, Bin Xu, Chuan Li, Qunpeng He, Tingting Zhu, Rong Fan, Dehua Gong
Valproic acid (VPA) is widely used for treatment of epilepsy. However, its overdose can cause intoxication and could be life-threatening. Due to lack of specific antidote and poorness of endogenous clearance, extracorporeal treatment for severe intoxication cases is indicated. Here, we report a case of severe intoxication of VPA which was successfully treated with liver support therapy. A previously healthy woman was admitted due to coma and hypotension after intentional ingestion of 20 g of sodium valproate...
May 8, 2017: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/28480408/effects-of-combretum-hereroense-and-canthiummundianum-water-extracts-on-production-and-expression-of-interleukin-4
#17
Amidou Samie, Nditsheni Madzie
BACKGROUND: Combretum hereroense and Canthium mundianum are two plants commonly used by traditional healers in the Northern region of Limpopo, South Africa for the treatment of diarrhea and inflammation. In the present study, the effects of their water extracts on the production and expression of interleukin-4 by peripheral blood mononuclear cells (PBMC'S) from HIV positive and negative individuals was evaluated. MATERIALS AND METHODS: Blood samples were collected from both HIV positive and HIV negative volunteers and were used for the purification of Peripheral blood mononuclear cells (PBMC)...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28477201/isolation-and-purification-of-th9-cells-for-the-study-of-inflammatory-diseases-in-research-and-clinical-settings
#18
Patricia Keating, James X Hartmann
Th9 cells are associated with atopic and inflammatory diseases, and their increased levels and function correlate with the severity of symptoms in various inflammatory disorders including asthma, food allergy, atopic dermatitis, ulcerative colitis, and psoriatic arthritis. Thus, clinical trials are warranted to evaluate the role of Th9 cells in allergic diseases with the goal of controlling these ailments.Circulating T cells (naïve or memory CD4+ T cells) purified from human blood and expanded using anti-CD3 and anti-CD28 antibodies can be treated with appropriate cytokines in order to polarize them to the Th9 phenotype as evidenced by their production of IL-9...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28476593/isolation-purification-and-characterization-of-staphylocoagulase-a-blood-coagulating-protein-from-staphylococcus-sp-mbbjp-s43
#19
Moonmee Bharadwaz, Prasenjit Manna, Dhrubajyoti Das, Niren Dutta, Jatin Kalita, Balagopalan Unni, Hari Prasanna Deka Boruah
Staphylocoagulase, a protein produced by S. aureus, play major role in blood coagulation and investigations are in advance to discover more staphylocoagulase producing species. The present study demonstrates the identification of a coagulase producing bacteria and isolation, purification and characterization of the protein. The bacteria was identified using 16S rDNA sequencing and phylogenetic investigation, classified the bacteria as Staphylococcus sp. MBBJP S43 with Genbank accession number KX907247. Tube test and Chromozym TH assay were used to study enzyme activity and comparison was made with five standard coagulase positive strains...
May 2, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28473885/prospects-and-limitations-of-antibody-mediated-clearing-of-lipoproteins-from-blood-plasma-prior-to-nanoparticle-tracking-analysis-of-extracellular-vesicles
#20
Morten Mørk, Aase Handberg, Shona Pedersen, Malene M Jørgensen, Rikke Bæk, Morten K Nielsen, Søren R Kristensen
Introduction: Nanoparticle tracking analysis (NTA) enables measurement of extracellular vesicles (EVs) but lacks the ability to distinct between EVs and lipoproteins which are abundantly present in blood plasma. Limitations in ultracentrifugation and size exclusion chromatography applied for EV isolation may result in inadequate EV purification and preservation. In this proof of concept study, we aimed to evaluate the potential of antibody-mediated removal of lipoproteins from plasma prior to extracellular vesicle (EV) analysis by nanoparticle tracking analysis (NTA)...
2017: Journal of Extracellular Vesicles
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