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https://www.readbyqxmd.com/read/27914030/active-compounds-extracted-from-extra-virgin-olive-oil-counteract-mesothelial-to-mesenchymal-transition-of-peritoneal-mesothelium-cells-exposed-to-conventional-peritoneal-dialysate-in-vitro-and-in-vivo-evidences
#1
S Lupinacci, G Toteda, D Vizza, A Perri, C Benincasa, A Mollica, A La Russa, P Gigliotti, F Leone, D Lofaro, M Bonofiglio, E Perri, R Bonofiglio
During peritoneal dialysis (PD), peritoneal mesothelial cells undergo a transition from an epithelial phenotype to a mesenchymal phenotype that, together with the inflammatory process, promotes tissue fibrosis and a failure of peritoneal membrane function. To date, there is no definitive treatment for the progressive thickening and angiogenesis of the peritoneal membrane associated with PD. In this study we tested, in vitro and in vivo, the ability of active compounds extracted from extra virgin olive oil (AC-EVOO) to counteract the mesothelial-to-mesenchymal transition process (MMT) observed in mesothelial cells chronically exposed to the conventional peritoneal dialysate (DL)...
December 2, 2016: Journal of Nephrology
https://www.readbyqxmd.com/read/27913774/gravity-flow-in-proposed-enteral-tube-small-bore-connectors
#2
Ryan T Hurt, Lisa M Epp, Adele K Pattinson, Wanda M Duellman, Stephen M Corner, Manpreet S Mundi
BACKGROUND: Enteral nutrition (EN) misconnections have been identified as a serious and potential deadly problem. An international effort led by EN industry leaders has developed a small-bore enteral connector (ENFit) that in theory will reduce the frequency of misconnections. Despite the potential benefit of preventing misconnections, the full impact of adoption of the ENFit connector is unknown. To assess the impact of transitioning to ENFit on our home EN (HEN) patients, the current study evaluated gravity feeding comparing 2 proposed small-bore connectors to the legacy (current connector) using various commercial formulas...
December 2, 2016: Nutrition in Clinical Practice
https://www.readbyqxmd.com/read/27913429/heritability-of-the-effective-connectivity-in-the-resting-state-default-mode-network
#3
Junhai Xu, Xuntao Yin, Haitao Ge, Yan Han, Zengchang Pang, Baolin Liu, Shuwei Liu, Karl Friston
The default mode network (DMN) is thought to reflect endogenous neural activity, which is considered as one of the most intriguing phenomena in cognitive neuroscience. Previous studies have found that key regions within the DMN are highly interconnected. Here, we characterized the genetic influences on causal or directed information flow within the DMN during the resting state. In this study, we recruited 46 pairs of twins and collected fMRI imaging data using a 3.0 T scanner. Dynamic causal modeling was conducted for each participant, and a structural equation model was used to calculate the heritability of DMN in terms of its effective connectivity...
November 23, 2016: Cerebral Cortex
https://www.readbyqxmd.com/read/27913338/intracardiac-vortex-dynamics-by-high-frame-rate-doppler-vortography-i-in-vivo-i-comparison-with-vector-flow-mapping-and-4-d-flow-mri
#4
Julia Faurie, Mathilde Baudet, Kondo Claude Assi, Dominique Auger, Guillaume Gilbert, Francois Tournoux, Damien Garcia
Recent studies have suggested that intracardiac vortex flow imaging could be of clinical interest to early diagnose the diastolic heart function. Doppler vortography has been intro-duced as a simple color Doppler method to detect and quantify intraventricular vortices. This method is able to locate a vortex core based on the recognition of an antisymmetric pattern in the Doppler velocity field. Because the heart is a fast-moving organ, high frame rates are needed to decipher the whole blood vortex dynamics during diastole...
November 29, 2016: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
https://www.readbyqxmd.com/read/27913304/effects-of-nongenetic-factors-on-immune-cell-dynamics-in-early-childhood-the-generation-r-study
#5
Diana van den Heuvel, Michelle A E Jansen, Kazem Nasserinejad, Willem A Dik, Ellen G van Lochem, Liesbeth E Bakker-Jonges, Halima Bouallouch-Charif, Vincent W V Jaddoe, Herbert Hooijkaas, Jacques J M van Dongen, Henriëtte A Moll, Menno C van Zelm
BACKGROUND: . Numbers of blood leukocyte subsets are highly dynamic in childhood and differ greatly between individuals. Inter-individual variation is only partly accounted for by genetic factors. OBJECTIVE: Determine which nongenetic factors affect the dynamics of innate leukocytes, and naive and memory lymphocyte subsets. METHODS: . We performed six-color flow cytometry and linear mixed effect modeling to define the dynamics of 62 leukocyte subsets from birth to 6 years of age in 1,182 children with one to five measurements per individual...
November 29, 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27913185/mir-346-promotes-the-biological-function-of-breast-cancer-cells-by-targeting-srcin1-and-reduces-chemosensitivity-to-docetaxel
#6
Fan Yang, Long-Ji Luo, Lei Zhang, Dan-Dan Wang, Su-Jin Yang, Li Ding, Jian Li, Dan Chen, Rong Ma, Jian-Zhong Wu, Jin-Hai Tang
MicroRNAs (miRNAs) are a class of highly conserved small noncoding RNAs that play pivotal roles at the post-transcriptional level in the biological function of various cancers, including breast cancer. In our study, miR-346 mimic, inhibitor, negative control or si-SRCIN1 were transfected into MCF-7 and MCF-7/Doc cells, respectively. Quantitative real time PCR (qRT-PCR) was used to measure miR-346 and SRCIN1 mRNA expressions and western blot was used to detect the expression of SRCIN1 in protein level. CCK-8 and colony formation were employed to verify cell viability and proliferation...
November 29, 2016: Gene
https://www.readbyqxmd.com/read/27913132/rapid-identification-of-candida-spp-frequently-involved-in-invasive-mycoses-by-using-flow-through-hybridization-and-gene-chip-fhgc-technology
#7
Chen Li, Xiurong Ding, Zhizhong Liu, Juanjuan Zhu
The incidence of invasive fungal infections in immunocompromised patients has increased in recent decades. Rapid and accurate identification of these pathogenic fungi is crucial for initiating a timely, safe, and effective antifungal therapy. Here we developed a microarray based on flow-through hybridization gene chip technology. The microarray was tested for its specificity using a panel of reference and blinded clinical isolates. The results proved that this microarray was highly discriminative, leading to the unequivocal identification of each species, including Candida famata and the highly related species Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis...
November 29, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27913054/development-of-a-multi-stakeholder-partnership-to-improve-access-to-and-delivery-of-neurosurgical-services-in-ontario
#8
Sunjay Sharma, Des Bohn, Iphigenia Mikroyiannakis, Joslyn Trowbridge, Donna Thompson, Robert Bell, James Rutka
Neurosurgical emergencies require expedient access to definitive care at neurosurgical centers. Neurosurgical resources in province of Ontario are highly centralized, and subsequently, most patients with neurosurgical emergencies will present to non-neurosurgical centers. From 2000-2010, metrics demonstrated the organization of neurosurgical resources might not be optimal. In response to this a program entitled Provincial Neurosurgery Ontario (PNO)- was formed to address these issues in cooperation with neurosurgeons, hospitals and the provincial government...
November 22, 2016: Health Policy
https://www.readbyqxmd.com/read/27912200/is-cha2ds2-vasc-appropriate-for-hyperthyroid-patients-with-atrial-fibrillation-implications-of-adding-a-transesophageal-echocardiography-evaluation
#9
Marcus Vinicius Leitão de Souza, Patricia de Fátima Dos Santos Teixeira, Mario Vaisman, Sergio Salles Xavier
BACKGROUND: Anticoagulation remains a controversial issue among hyperthyroid patients with atrial fibrillation (AF). We aimed to evaluate the prevalence of the thrombogenic milieu (TM), detected using transesophageal echocardiography (TEE), among patients with AF related to hyperthyroidism, and to correlate these findings with the clinical embolic risk classification (CHA2DS2-VASc). METHODS: CHA2DS2-VASc score, thyroid hormonal status, time since hyperthyroidism diagnosis, transthoracic echocardiography (TTE) and TEE were assessed in 47 consecutive patients aged between 18 and 65years with AF related to hyperthyroidism...
November 10, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27912182/influence-of-the-gas-liquid-flow-configuration-in-the-absorption-column-on-photosynthetic-biogas-upgrading-in-algal-bacterial-photobioreactors
#10
Alma Toledo-Cervantes, Cindy Madrid-Chirinos, Sara Cantera, Raquel Lebrero, Raúl Muñoz
The potential of an algal-bacterial system consisting of a high rate algal pond (HRAP) interconnected to an absorption column (AC) via recirculation of the cultivation broth for the upgrading of biogas and digestate was investigated. The influence of the gas-liquid flow configuration in the AC on the photosynthetic biogas upgrading process was assessed. AC operation in a co-current configuration enabled to maintain a biomass productivity of 15gm(-2)d(-1), while during counter-current operation biomass productivity decreased to 8...
November 23, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27911995/allometry-data-and-equations-for-coastal-marsh-plants
#11
Meng Lu, Joshua S Caplan, Jonathan D Bakker, J Adam Langley, Thomas J Mozdzer, Bert G Drake, J Patrick Megonigal
Coastal marshes are highly valued for ecosystem services such as protecting inland habitats from storms, sequestering carbon, removing nutrients and other pollutants from surface water, and providing habitat for fish, shellfish, and birds. Because plants largely determine the structure and function of coastal marshes, quantifying plant biomass is essential for evaluating these ecosystem services, understanding the biogeochemical processes that regulate ecosystem function, and forecasting tidal wetland responses to accelerated sea level rise...
December 2016: Ecology
https://www.readbyqxmd.com/read/27911823/continuous-flow-synthesis-of-zsm-5-zeolite-on-the-order-of-seconds
#12
Zhendong Liu, Kotatsu Okabe, Chokkalingam Anand, Yasuo Yonezawa, Jie Zhu, Hiroki Yamada, Akira Endo, Yutaka Yanaba, Takeshi Yoshikawa, Koji Ohara, Tatsuya Okubo, Toru Wakihara
The hydrothermal synthesis of zeolites carried out in batch reactors takes a time so long (typically, on the order of days) that the crystallization of zeolites has long been believed to be very slow in nature. We herein present a synthetic process for ZSM-5, an industrially important zeolite, on the order of seconds in a continuous flow reactor using pressurized hot water as a heating medium. Direct mixing of a well-tuned precursor (90 °C) with the pressurized water preheated to extremely high temperature (370 °C) in the millimeter-sized continuous flow reactor resulted in immediate heating to high temperatures (240-300 °C); consequently, the crystallization of ZSM-5 in a seed-free system proceeded to completion within tens of or even several seconds...
November 28, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27911466/transparent-nanofibrous-mesh-self-assembled-from-molecular-legos-for-high-efficiency-air-filtration-with-new-functionalities
#13
Varun Kumar Singh, Sai Kishore Ravi, Wanxin Sun, Swee Ching Tan
Alarming levels of particulate matter pollution in air pose a serious health threat in several countries, therefore intriguing a strong need for an economic and a viable technology of air filtration. Current air purification technology is rather expensive with certain types even having the risk of emitting hazardous by-products. The authors have developed a multifunctional air filter inspired from the nasal hairs possessing an ability to specifically trap/exhale the foreign particles and allergens while still letting the air flow...
December 2, 2016: Small
https://www.readbyqxmd.com/read/27911421/-analysis-of-changes-in-central-macular-thickness-based-on-optical-coherence-tomography-angiography-findings-in-retinal-vein-occlusion
#14
M V Budzinskaya, A V Shelankova, M A Mikhailova, A A Plyukhova, N M Nurieva, A V Fomin
AIM: To investigate the relationship between OCT angiography measurements and central fundus changes in patients with retinal vein occlusion (RVO). MATERIAL AND METHODS: The study enrolled 21 RVO patients aged from 55 to 86 years (69.9±2.28 years), including 8 patients with ischemic central RVO (I-CRVO; 73.6±3.4 years on average) and 13 patients with branch RVO (BRVO; 67.6±3.0 years on average). Of the latter, 8 cases were ischemic (I-BRVO) and 5 non-ischemic (NI-BRVO)...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27911410/experimental-infection-with-listeria-monocytogenes-as-a-model-for-studying-host-interferon-%C3%AE-responses
#15
Jeeyoon Jennifer Ahn, Thirumahal Selvanantham, Monan Angela Zhang, Thierry Mallevaey, Shannon E Dunn
L. monocytogenes is a gram-positive bacterium that is a cause of food borne disease in humans. Experimental infection of mice with this pathogen has been highly informative on the role of innate and adaptive immune cells and specific cytokines in host immunity against intracellular pathogens. Production of IFN-γ by innate cells during sublethal infection with L. monocytogenes is important for activating macrophages and early control of the pathogen(1-3). In addition, IFN-γ production by adaptive memory lymphocytes is important for priming the activation of innate cells upon reinfection(4)...
November 16, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911407/preparation-of-cd4-t-cells-for-analysis-of-gd3-and-gd2-ganglioside-membrane-expression-by-microscopy
#16
Tania M Villanueva-Cabello, Iván Martinez-Duncker
The methods described herein for activation of naïve CD4(+) T cells in suspension and their adherence in coverslips for confocal microscopy analysis allow the spatial localization and visualization of gangliosides involved in CD4(+) T cell activation, that complement expression profiling experiments such as flow cytometry, western blotting or real-time PCR. The quantification of ganglioside expression through flow cytometry and their cellular localization through microscopy can be obtained by the use of anti-ganglioside antibodies with high affinity and specificity...
November 8, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911373/the-mouse-isolated-perfused-kidney-technique
#17
Jan Czogalla, Frank Schweda, Johannes Loffing
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. This is a prerequisite for studying the physiology of the isolated organ and for many innovative applications that may be possible in the future, including perfusion decellularization for kidney bioengineering or the administration of anti-rejection or genome-editing drugs in high doses to prime the kidney for transplantation. During the time of the perfusion, the kidney can be manipulated, renal function can be assessed, and various pharmaceuticals administered...
November 17, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911067/-vascular-interventional-radiology-a-fundamental-procedure-for-the-management-of-paediatric-trauma
#18
E Bordón Cabrera, A Laín, R Gander, M Pérez Lafuente, I Díez Miranda, C G Fontecha, L Seidler, I Delgado, S Cañadas Palazón, J Lloret
INTRODUCTION: The management of active bleeding with haemodinamic lability in the paediatric trauma patient is difficult and generally leads to damage control surgery. Vascular Interventional Radiology (VIR) techniques are useful for the diagnosis as for the definitive treatment. AIM: The aim of our study was to describe our experience and evaluate effectiveness of VIR in the management of the paediatric trauma patient with active bleeding signs. METHODS: Retrospective analysis (2003-2014) of politraumatic patients who showed contrast blush on computed tomography and then treated by VIR techniques...
January 25, 2016: Cirugía Pediátrica: Organo Oficial de la Sociedad Española de Cirugía Pediátrica
https://www.readbyqxmd.com/read/27910898/hydrogen-overproducing-nitrogenases-obtained-by-random-mutagenesis-and-high-throughput-screening
#19
Emma Barahona, Emilio Jiménez-Vicente, Luis M Rubio
When produced biologically, especially by photosynthetic organisms, hydrogen gas (H2) is arguably the cleanest fuel available. An important limitation to the discovery or synthesis of better H2-producing enzymes is the absence of methods for the high-throughput screening of H2 production in biological systems. Here, we re-engineered the natural H2 sensing system of Rhodobacter capsulatus to direct the emission of LacZ-dependent fluorescence in response to nitrogenase-produced H2. A lacZ gene was placed under the control of the hupA H2-inducible promoter in a strain lacking the uptake hydrogenase and the nifH nitrogenase gene...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27910892/dynamic-microenvironment-induces-phenotypic-plasticity-of-esophageal-cancer-cells-under-flow
#20
Gizem Calibasi Kocal, Sinan Güven, Kira Foygel, Aaron Goldman, Pu Chen, Shiladitya Sengupta, Ramasamy Paulmurugan, Yasemin Baskin, Utkan Demirci
Cancer microenvironment is a remarkably heterogeneous composition of cellular and non-cellular components, regulated by both external and intrinsic physical and chemical stimuli. Physical alterations driven by increased proliferation of neoplastic cells and angiogenesis in the cancer microenvironment result in the exposure of the cancer cells to elevated levels of flow-based shear stress. We developed a dynamic microfluidic cell culture platform utilizing eshopagael cancer cells as model cells to investigate the phenotypic changes of cancer cells upon exposure to fluid shear stress...
December 2, 2016: Scientific Reports
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