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osteomyelitis; gene

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https://www.readbyqxmd.com/read/29739668/whole-genome-comparison-of-high-and-low-virulent-staphylococcus-aureus-isolates-inducing-implant-associated-bone-infections
#1
Gopala Krishna Mannala, Julian Koettnitz, Walid Mohamed, Ursula Sommer, Katrin Susanne Lips, Cathrin Spröer, Boyke Bunk, Jörg Overmann, Torsten Hain, Christian Heiss, Eugen Domann, Volker Alt
Staphylococcus aureus can cause wide range of infections from simple soft skin infections to severe endocarditis, bacteremia, osteomyelitis and implant associated bone infections (IABI). The focus of the present investigation was to study virulence properties of S. aureus isolates from acute and chronic IABI by means of their in vivo lethality, in vitro osteoblasts invasion, biofilm formation and subsequently whole genome comparison between high and low virulent strains. Application of insect infection model Galleria mellonella revealed high, intermediate and low virulence phenotypes of these clinical isolates, which showed good correlation with osteoblast invasion and biofilm formation assays...
April 25, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29723617/cd14-monocytes-contribute-to-inflammation-in-chronic-nonbacterial-osteomyelitis-cno-through-increased-nlrp3-inflammasome-expression
#2
D Brandt, E Sohr, J Pablik, A Schnabel, F Kapplusch, K Mäbert, J H Girschick, H Morbach, F Thielemann, S R Hofmann, C M Hedrich
The pathophysiology of chronic nonbacterial osteomyelitis (CNO) remains incompletely understood. Increased NLRP3 inflammasome activation and IL-1β release in monocytes from CNO patients was suggested to contribute to bone inflammation. Here, we dissect immune cell infiltrates and demonstrate the involvement of monocytes across disease stages. Differences in cell density and immune cell composition may help to discriminate between BOM and CNO. However, differences are subtle and infiltrates vary in CNO. In contrast to other cells involved, monocytes are a stable element during all stages of CNO, which makes them a promising candidate in the search for "drivers" of inflammation...
April 30, 2018: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/29691335/novel-insights-into-staphylococcus-aureus-deep-bone-infections-the-involvement-of-osteocytes
#3
Dongqing Yang, Asiri R Wijenayaka, Lucian B Solomon, Stephen M Pederson, David M Findlay, Stephen P Kidd, Gerald J Atkins
Periprosthetic joint infection (PJI) is a potentially devastating complication of orthopedic joint replacement surgery. PJI with associated osteomyelitis is particularly problematic and difficult to cure. Whether viable osteocytes, the predominant cell type in mineralized bone tissue, have a role in these infections is not clear, although their involvement might contribute to the difficulty in detecting and clearing PJI. Here, using Staphylococcus aureus , the most common pathogen in PJI, we demonstrate intracellular infection of human-osteocyte-like cells in vitro and S...
April 24, 2018: MBio
https://www.readbyqxmd.com/read/29624568/resveratrol-attenuates-lipopolysaccharides-lps-induced-inhibition-of-osteoblast-differentiation-in-mc3t3-e1-cells
#4
Jun Ma, Zhu Wang, Jianquan Zhao, WuJun Miao, TianWen Ye, Aimin Chen
BACKGROUND LPS-inhibited osteoblastic differentiation plays an important role in the pathogenesis of osteomyelitis. Thus, searching for drugs that affect LPS-mediated osteoblastic differentiation may be crucial in developing therapies for osteomyelitis. The purpose of this study was to investigate the role and mechanisms of resveratrol, a natural polyphenol present in red wine, on LPS-inhibited osteoblastic differentiation. MATERIAL AND METHODS Cell viability was measured by MMT assay. Mitochondrial ATP levels, membrane potential, and superoxide production were measured to evaluate the effects of LPS and resveratrol on mitochondrial functions in osteoblast-like MC3T3-E1 cells...
April 6, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29620718/kingella-kingae-as-the-main-cause-of-septic-arthritis-importance-of-molecular-diagnosis
#5
María Belén Hernández-Rupérez, María Del Carmen Suárez-Arrabal, Ángel Villa-García, Sara Zarzoso-Fernández, Marisa Navarro-Gómez, Mar Santos-Sebastián, Azucena García-Martín, Mercedes Marín, Felipe González-Martínez, Javier Narbona-Cárceles, Paloma Cervera-Bravo, José Luis González-López, Teresa Hernández-Sampelayo, Jesús Saavedra-Lozano
BACKGROUND: Kingella kingae is an emergent pathogen causing septic arthritis (SA) in children. OBJECTIVES: To analyze the etiology of SA in children before and after the implementation of universal 16S rRNA gene polymerase chain reaction and sequencing (16SPCR) in synovial fluid. METHODS: Children ≤ 14 years with acute SA from a Madrid cohort (2002-2013), were reviewed. Differences in etiology were analyzed before (Period 1) and after (Period 2) the implementation of bacterial 16SPCR in 2009...
March 31, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29564946/virulence-factor-genes-in-staphylococcus-aureus-isolated-from-diabetic-foot-soft-tissue-and-bone-infections
#6
Gerardo Víquez-Molina, Javier Aragón-Sánchez, Cristian Pérez-Corrales, Christian Murillo-Vargas, María Eugenia López-Valverde, Benjamin A Lipsky
The aim of this study is to describe the presence of genes encoding for 4 virulence factors (pvl, eta, etb, and tsst), as well as the mecA gene conferring resistance to beta-lactam antibiotics, in patients with diabetes and a staphylococcal foot infection. We have also analyzed whether isolates of Staphylococcus aureus from bone infections have a different profile for these genes compared with those from exclusively soft tissue infections. In this cross-sectional study of a prospectively recruited series of patients admitted to the Diabetic Foot Unit, San Juan de Dios Hospital, San José, Costa Rica with a moderate or severe diabetic foot infection (DFI), we collected samples from infected soft tissue and from bone during debridement...
March 2018: International Journal of Lower Extremity Wounds
https://www.readbyqxmd.com/read/29517430/clinicopathologic-features-of-infection-with-novel-brucella-organisms-in-captive-waxy-tree-frogs-phyllomedusa-sauvagii-and-colorado-river-toads-incilius-alvarius
#7
Kelly E Helmick, Michael M Garner, Jack Rhyan, Daniel Bradway
Two novel and distinct Brucella strains were recovered from 5 of 10 adult, sex undetermined, captive waxy tree frogs ( Phyllomedusa sauvagii) and two of five adult, sex undetermined, captive Colorado river toads ( Incilius alvarius) held in a zoologic collection with clinical and pathologic findings of bacterial disease. These amphibians originated from three separate private breeding facilities over several years and exhibited disease 9-49 mo following release from quarantine. Common presenting signs were vague but included focal abscessation, weight loss, change in coloration, anorexia, and decreased perching...
March 2018: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/29451003/escherichia-coli-isolates-from-femoral-bone-marrow-of-broilers-exhibit-diverse-pheno-and-genotypic-characteristics-that-do-not-correlate-with-macroscopic-lesions-of-bacterial-chondronecrosis-with-osteomyelitis
#8
Barbara Gaußmann, Claudia Hess, Beatrice Grafl, Mercedes Kovacs, Salome Troxler, Beatrix Stessl, Michael Hess, Surya Paudel
The pheno- and genotypic relatedness among Escherichia coli isolates from broilers with and without macroscopic lesions of the femoral head were investigated. In total, 219 isolates obtained from the bone marrow were characterized by serotyping, antimicrobial resistance (AMR) profiles, phylogenetic grouping, detection of virulence-associated genes (VAGs) and pulsed-field gel electrophoresis (PFGE). Serotyping revealed that 48.4% of the isolates were assigned to one of the three serotypes (O78:K80: 21.0%, O2:K1: 18...
June 2018: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/29321407/vertebral-osteomyelitis-caused-by-helicobacter-cinaedi-identified-using-broad-range-polymerase-chain-reaction-with-sequencing-of-the-biopsied-specimen
#9
Ryota Hase, Takuya Hirooka, Takashi Itabashi, Yasunobu Endo, Yoshihito Otsuka
A 65-year-old man presented with gradually exacerbating low back pain. Magnetic resonance imaging revealed vertebral osteomyelitis in the Th11-L2 vertebral bodies and discs. The patient showed negative findings on conventional cultures. Direct broad-range polymerase chain reaction (PCR) with sequencing of the biopsied specimen had the highest similarity to the 16S rRNA gene of Helicobacter cinaedi. This case suggests that direct broad-range PCR with sequencing should be considered when conventional cultures cannot identify the causative organism of vertebral osteomyelitis, and that this method may be particularly useful when the pathogen is a fastidious organism, such as H...
January 11, 2018: Internal Medicine
https://www.readbyqxmd.com/read/29234317/panton-valentine-leucocidin-pvl-as-a-potential-indicator-for-prevalence-duration-and-severity-of-staphylococcus-aureus-osteomyelitis
#10
Bei Jiang, Yinan Wang, Zihan Feng, Lei Xu, Li Tan, Shuang Zhao, Yali Gong, Cheng Zhang, Xiaoqiang Luo, Shu Li, Xiancai Rao, Yizhi Peng, Zhao Xie, Xiaomei Hu
Staphylococcus aureus is the most common cause of the difficult-to-treat osteomyelitis (OM). To better diagnose and manage S. aureus OM, especially for severe and long duration cases, indicators for risk prediction and severity evaluation are needed. Here, 139 clinical S. aureus isolates from orthopedic infections were divided into OM group (60 isolates from 60 OM patients) and non-OM group (79 isolates from 79 non-OM patients). Molecular types, antimicrobial susceptibility, and virulence factor profiles were evaluated and compared between the two groups to identify potential indicators associated with the prevalence of S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29231857/biodegradable-scaffolds-for-bone-regeneration-combined-with-drug-delivery-systems-in-osteomyelitis-therapy
#11
REVIEW
Rossella Dorati, Antonella DeTrizio, Tiziana Modena, Bice Conti, Francesco Benazzo, Giulia Gastaldi, Ida Genta
A great deal of research is ongoing in the area of tissue engineering (TE) for bone regeneration. A possible improvement in restoring damaged tissues involves the loading of drugs such as proteins, genes, growth factors, antibiotics, and anti-inflammatory drugs into scaffolds for tissue regeneration. This mini-review is focused on the combination of the local delivery of antibiotic agents with bone regenerative therapy for the treatment of a severe bone infection such as osteomyelitis. The review includes a brief explanation of scaffolds for bone regeneration including scaffolds characteristics and types, a focus on severe bone infections (especially osteomyelitis and its treatment), and a literature review of local antibiotic delivery by the combination of scaffolds and drug-delivery systems...
December 12, 2017: Pharmaceuticals
https://www.readbyqxmd.com/read/29227579/obesity-type-2-diabetes-increases-inflammation-periosteal-reactive-bone-formation-and-osteolysis-during-staphylococcus-aureus-implant-associated-bone-infection
#12
Christopher W Farnsworth, Eric M Schott, Abigail M Benvie, Jacob Zukoski, Stephen L Kates, Edward M Schwarz, Steven R Gill, Michael J Zuscik, Robert A Mooney
Obese and type 2 diabetic (T2D) patients have a fivefold increased rate of infection following placement of an indwelling orthopaedic device. Though implant infections are associated with inflammation, periosteal reactive bone formation, and osteolysis, the effect of obesity/T2D on these complicating factors has not been studied. To address this question, C57BL/6J mice were fed a high fat diet (60% Kcal from fat) to induce obesity/T2D, or a control diet (10% Kcal from fat) for 3 months, and challenged with a transtibial pin coated with a bioluminescent USA300 strain of S...
December 11, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29218100/-staphylococcus-aureus-peptidoglycan-promotes-osteoclastogenesis-via-tlr2-mediated-activation-of-the-nf-%C3%AE%C2%BAb-nfatc1-signaling-pathway
#13
Faqi Cao, Wu Zhou, Guohui Liu, Tian Xia, Mengfei Liu, Bobin Mi, Yi Liu
Staphylococcus aureus ( S. aureus ) peptidoglycan (PGN-sa), the major cell wall component of S. aureus , has been demonstrated to be an important virulence factor in the pathogenesis of S. aureus -induced osteomyelitis. However, the exact role of PGN-sa in osteoclastogenesis during S. aureus -induced osteomyelitis and its underlying molecular mechanisms remain unclear. In this study, we found that PGN-sa promoted receptor activator of nuclear factor-κB (NF-κB) ligand (RANKL)-induced osteoclast formation. Quantitative real-time polymerase chain reaction results showed that the mRNA expression of osteoclast-specific marker genes, including tartrate-resistant acid phosphatase, cathepsin K, matrix metalloproteinase-9, and calcitonin receptor was upregulated by PGN-sa treatment...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29204271/genome-sequencing-and-analysis-of-the-first-spontaneous-nanosilver-resistant-bacterium-proteus-mirabilis-strain-scdr1
#14
Amr T M Saeb, Khalid A Al-Rubeaan, Mohamed Abouelhoda, Manojkumar Selvaraju, Hamsa T Tayeb
Background: P. mirabilis is a common uropathogenic bacterium that can cause major complications in patients with long-standing indwelling catheters or patients with urinary tract anomalies. In addition, P. mirabilis is a common cause of chronic osteomyelitis in Diabetic foot ulcer (DFU) patients. We isolated P. mirabilis SCDR1 from a Diabetic ulcer patient. We examined P. mirabilis SCDR1 levels of resistance against Nanosilver colloids, the commercial Nanosilver and silver containing bandages and commonly used antibiotics...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29164118/infections-caused-by-antimicrobial-drug-resistant-saprophytic-gram-negative-bacteria-in-the-environment
#15
Eva Raphael, Lee W Riley
Background: Drug-resistance genes found in human bacterial pathogens are increasingly recognized in saprophytic Gram-negative bacteria (GNB) from environmental sources. The clinical implication of such environmental GNBs is unknown. Objectives: We conducted a systematic review to determine how often such saprophytic GNBs cause human infections. Methods: We queried PubMed for articles published in English, Spanish, and French between January 2006 and July 2014 for 20 common environmental saprophytic GNB species, using search terms "infections," "human infections," "hospital infection...
2017: Frontiers in Medicine
https://www.readbyqxmd.com/read/29078156/an-unusual-osteomyelitis-caused-by-moraxella-osloensis-a-case-report
#16
Nidal J Alkhatib, Manaf H Younis, Ahmad S Alobaidi, Nebal M Shaath
INTRODUCTION: Moraxella osloensis is a gram-negative coccobacillus, that is saprophytic on skin and mucosa, and rarely causing human infections. Reported cases of human infections usually occur in immunocompromised patients. PRESENTATION OF CASE: We report the second case of M. osloensis-caused-osteomyelitis in literature, occurring in a young healthy man. The organism was identified by sequencing analysis of the 16S ribosomal RNA gene. Our patient was treated successfully with surgical debridement and intravenous third-generation cephalosporins...
2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/29054550/-mlva-analysis-and-virulence-profile-of-community-acquired-methicillin-resistant-staphylococcus-aureus-causing-infections-in-paraguayan-children
#17
Fátima Rodríguez Acosta, Wilma D Basualdo Acuña, Héctor Castro, Ana Campuzano, María L Macchi, Juana Ortellado de Canese, Patricia Almada, Mónica Rodríguez, Lorena Grau, Gladys Velázquez, Carmen Espínola, Gloria C Samudio Domínguez, Gloria Gómez, Letizia Carpinelli, Rosa M Guillén Fretes
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is one of the first causes of skin and soft tissue infections, and can also produce severe diseases such as osteomyelitis and pneumonia. The aim of this descriptive study was to determine the SCCmec type and virulence profile and to study the genetic diversity by MLVA analysis of 21 CA-MRSA isolates that infected Paraguayan children in 2010. The SCCmec type and virulence factors were performed by PCR and genetic diversity by MLVA (multiple locus variable analysis)...
October 17, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29049163/examining-the-association-of-mmp-1-gene-1607-2g-1g-and-519-a-g-polymorphisms-with-the-risk-of-osteomyelitis-a-case-control-study
#18
Qingzhu Kong, Yu Jin, Shi Yan, Yin Wang, Jingxin Zhao, Zhen Feng, Junqiang Wei, Yu Wang, Lingwei Kong, Litao Guo, Jianing Yang
To investigate the effects of matrix metalloproteinase-1 (MMP-1) gene polymorphisms on the onset of osteomyelitis in Chinese Han population.In all, 80 osteomyelitis patients (case group) and 81 healthy people (control group) were recruited into this case-control study. Polymerase chain reaction-restriction fragment length polymorphism method was utilized to examine the genotypes of MMP-1 polymorphisms (-1607 2G/1G and -519A/G) in the 2 groups. Genotype and allele differences between the case and control groups were analyzed by chi-square test...
October 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29048488/clinical-and-molecular-features-of-mdr-livestock-associated-mrsa-st9-with-staphylococcal-cassette-chromosome-mecxii-in-humans
#19
Chih-Jung Chen, Tsai-Ling Yang Lauderdale, Chin-Te Lu, Yu-Yu Chuang, Chien-Chang Yang, Ting-Shu Wu, Chun-Yi Lee, Min-Chi Lu, Wen-Chien Ko, Yhu-Chering Huang
Objectives: Clonal complex (CC) 9 is a prevalent livestock-associated (LA) MRSA clone in Asia whose pathogenicity in humans remains unknown. Methods: In 2012, we identified a patient with CC9-MRSA infection linked to livestock. After screening 3328 clinical MRSA isolates from a national database, eight isolates (0.24%) collected between 1998 and 2012 were further confirmed to be of CC9. The detailed molecular features of the nine human CC9 strains and phylogenetic relatedness to animal CC9 strains were characterized with WGS...
January 1, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28942456/staphylococcus-aureus-induces-osteoclastogenesis-via-the-nf-%C3%AE%C2%BAb-signaling-pathway
#20
Li-Rong Ren, Zhi-Hua Wang, Hai Wang, Xiao-Qing He, Mu-Guo Song, Yong-Qing Xu
BACKGROUND Osteomyelitis is one of the refractory diseases encountered in orthopedics, while Staphylococcus aureus (S. aureus) is the most common causative organism in osteomyelitis. However, the precise mechanisms underlying the bone loss caused by S. aureus infection have not been well defined. Here, we investigated the effect of S. aureus on osteoclast differentiation and the probable molecular mechanism. MATERIAL AND METHODS RAW 264.7 cells were treated for 5 days with live S. aureus, inactivated S. aureus, and S...
September 24, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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