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https://www.readbyqxmd.com/read/28076526/perception-of-pre-and-post-hiv-test-counseling-among-patients-diagnosed-with-aids-in-adolescence-hiv-test-counseling-for-adolescents
#1
Stella Regina Taquette, Adriana de Oliveira Rodrigues, Lívia Rocha Bortolotti
Pre- and post-HIV test counseling has singular importance in adolescence, since it is a phase of great changes, and AIDS is an incurable chronic disease. In order to comprehend the perception of young people with AIDS diagnosed in adolescence regarding the counseling received upon testing, we conducted 39 in-depth interviews, with 23 in female patients and 16 male patients, and then analyzed the data with extensive reading, categorization and interpretation on a comprehensive basis. The results revealed that less than one third received pre-test counseling (30...
January 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28056489/use-of-high-frequency-ultrasound-to-study-the-prenatal-development-of-cranial-neural-tube-defects-and-hydrocephalus-in-gldc-deficient-mice
#2
Maria C Autuori, Yun J Pai, Daniel J Stuckey, Dawn Savery, Anna M Marconi, Valentina Massa, Mark F Lythgoe, Andrew J Copp, Anna L David, Nicholas D E Greene
OBJECTIVE: We used non-invasive high frequency ultrasound (HFUS) imaging to investigate embryonic brain development in a mouse model for neural tube defects (NTDs) and Non-Ketotic Hyperglycinemia (NKH). METHOD: Using HFUS, we imaged embryos carrying loss of function alleles of Gldc encoding glycine decarboxylase, a component of the glycine cleavage system in mitochondrial folate metabolism, which is known to be associated with cranial NTDs and NKH in humans. We serially examined the same litter during the second half of embryonic development and quantified cerebral structures...
January 5, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28004771/spatially-resolved-proteomics-rapid-quantitative-analysis-of-laser-capture-microdissected-alveolar-tissue-samples
#3
Geremy Clair, Paul D Piehowski, Teodora Nicola, Joseph A Kitzmiller, Eric L Huang, Erika M Zink, Ryan L Sontag, Daniel J Orton, Ronald J Moore, James P Carson, Richard D Smith, Jeffrey A Whitsett, Richard A Corley, Namasivayam Ambalavanan, Charles Ansong
Laser capture microdissection (LCM)-enabled region-specific tissue analyses are critical to better understand complex multicellular processes. However, current proteomics workflows entail several manual sample preparation steps and are challenged by the microscopic mass-limited samples generated by LCM, impacting measurement robustness, quantification and throughput. Here, we coupled LCM with a proteomics workflow that provides fully automated analysis of proteomes from microdissected tissues. Benchmarking against the current state-of-the-art in ultrasensitive global proteomics (FASP workflow), our approach demonstrated significant improvements in quantification (~2-fold lower variance) and throughput (>5 times faster)...
December 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27989075/maldi-imaging-mass-spectrometry-as-a-lipidomic-approach-to-heart-valve-research
#4
Peggi M Angel, Ahmed S Bayoumi, Robert B Hinton, Yan Ru Su, David Bichell, John E Mayer, H Scott Baldwin, Richard M Caprioli
BACKGROUND: Valvular disease is characterized in part by lipid deposition, but systematic analysis of the patterns of global lipid expression in healthy and diseased valve tissues are unknown. This is due in part to tissue limitations for lipidomic preparations and technologies for evaluating lipid distribution in tissues. The study aim was to examine the application of matrixassisted laser desorption ionization imaging mass spectrometry (MALDI IMS) to the aortic valve during development and disease, as an approach to detect and map lipids and ultimately better understand valve structure and function...
March 2016: Journal of Heart Valve Disease
https://www.readbyqxmd.com/read/27982391/using-the-bologna-score-to-assess-normal-delivery-healthcare
#5
Isaiane da Silva Carvalho, Rosineide Santana de Brito
OBJECTIVE: Describing the obstetric care provided in public maternity hospitals during normal labour using the Bologna Score in the city of Natal, Northeastern Brazil. METHOD: A quantitative cross-sectional study conducted with 314 puerperal women. Data collection was carried out consecutively during the months of March to July 2014. RESULTS: Prenatal care was provided to 95.9% of the mothers, beginning around the 1st trimester of pregnancy (72...
September 2016: Revista da Escola de Enfermagem da U S P
https://www.readbyqxmd.com/read/27910180/internal-migration-and-maternal-health-service-utilisation-in-jiangsu-china
#6
Hai Gu, Hua You, Weiqing Ning, Hua Zhou, Jianming Wang, Ying Lu, Jun Sun, Yun Kou, Hengjin Dong
OBJECTIVES: To investigate the use of maternal health care services by internal migrants in view of their migration status. METHODS: Cross-sectional household survey in two cities of Jiangsu Province. Questions elicited data on socioeconomic information and MHC service use (pre-natal examination, post-natal visit, pre-natal health education). Chi-square tests and multivariate logistic regression analyses were used to identify factors associated with MHC service use...
November 5, 2016: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/27891327/analysis-of-fetal-palate-to-assist-pre-natal-ultrasound
#7
Anjali Shastry, Yogitha Ravindranath, Roopa Ravindranath
INTRODUCTION: Cleft palate is one of the major facial congenital malformation in newborns. Pre-natal detection of this malformation is limited to detection of clefting of hard palate but isolated soft palate clefting still remains challenge for sonologists. As Indian literature is limited present study was attempted to provide dimensions and position of fetal palate by digitized images. AIM: To study dimensions, position and differences in parameters between second and third trimester fetuses...
October 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27856333/novel-homozygous-missense-mutation-in-aldh7a1-causes-neonatal-pyridoxine-dependent-epilepsy
#8
Emanuele G Coci, Luca Codutti, Christian Fink, Sophie Bartsch, Gunnar Grüning, Thomas Lücke, Ingo Kurth, Joachim Riedel
Pyridoxine dependent epilepsy (PDE) (OMIM#266100) is a neonatal form of epilepsy, caused by dysfunction of the enzyme α-aminoadipic semialdehyde dehydrogenase (ALDH7A1 or Antiquitin). This enzyme converts α-aminoadipic semialdehyde (α-AASA) into α-aminoadipate (AAA), a critical step in the lysine metabolism of the brain. ALDH7A1 dysfunction causes an accumulation of α-AASA and δ(1)-piperideine-6-carboxylic acid (P6C), which are in equilibrium with each other. P6C binds and inactivates pyridoxal 5'-phosphate (PLP), the active form of pyridoxine...
November 14, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27856159/pre-implantation-genetic-diagnosis
#9
REVIEW
Joanne Traeger-Synodinos
The aim of pre-implantation genetic diagnosis (PGD) is to characterize the genetic status of the cells (usually single cells) that have been biopsied from oocytes/zygotes or embryos created in vitro during assisted reproductive treatment. PGD is a multi-step procedure that requires close collaboration between gynaecologists who are experts in assisted reproduction, embryologists who are experts in micromanipulation of germ cells and in embryo biopsy and geneticists who are experts in genetic analysis at the single-cell level...
October 26, 2016: Best Practice & Research. Clinical Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/27847257/new-trend-in-the-epidemiology-of-thalassaemia
#10
REVIEW
Chi-Kong Li
Thalassaemia is the most common monogenic disorder worldwide. It is common in areas with prevalent malaria as thalassaemic red cells provide immunity against the parasite. The incidence of thalassaemia carriers is high in regions such as Mediterranean, Middle East, Indian subcontinent, Southeast Asia and South China. In the past few decades, migrants from the thalassaemia prevalent countries to non-prevalent countries, mainly North America and Central and North Europe, are rapidly increasing in number. The non-prevalent countries may not have established pre-natal screening system for thalassaemia...
October 26, 2016: Best Practice & Research. Clinical Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/27824070/modeling-the-development-of-the-post-natal-mouse-thymus-in-the-absence-of-bone-marrow-progenitors
#11
Daniela Zaharie, Radu D Moleriu, Felix A Mic
Many mathematical models have been published with the purpose of explaining aspects of T-cell development in the thymus. In this manuscript we adapted a four-compartment model of the thymus and used a range of mathematical approaches with the aim of explaining the dynamics of the four main thymocyte populations in the mouse thymus, from the emergence of the first fetal thymocyte until the death of the animal. At various pre-natal and post-natal stages we investigated experimentally the number and composition of thymocytes populations, their apoptosis and proliferation, along with data from literature, to create and validate the model...
November 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27809754/haemophilia-b-curative-fix-production-from-a-low-dose-ucoe-based-lentiviral-vector-following-hepatic-pre-natal-delivery
#12
Vincent Yu-Cheng Kao, Sonia Ferreira, Simon Nicholas Waddington, Michael Nicholas Antoniou
The ubiquitous chromatin opening element from the human HNRPA2B1-CBX3 housekeeping gene locus (A2UCOE) is able to provide stable and cell-to-cell reproducible levels of transgene expression regardless of target cell genome integration site with efficacy demonstrated in adult, embryonic and induced pluripotent stem cells and their differentiated progeny in vitro and in vivo. Here we evaluate the ability of A2UCOE-based lentiviral vectors to confer stable expression following pre-natal delivery in mice. Our results show stable post-natal A2UCOE-eGFP and A2UCOE-luciferase lentiviral vector presence in both the liver and haematopoietic system with concomitant persistence of expression demonstrating efficient transduction of both fetal hepatocytes and haematopoietic stem cells...
November 2, 2016: Current Gene Therapy
https://www.readbyqxmd.com/read/27807368/expression-of-the-fetal-hematopoiesis-regulator-fev-indicates-leukemias-of-prenatal-origin
#13
T-H Liu, Y-J Tang, Y Huang, L Wang, X-L Guo, J-Q Mi, L-G Liu, H Zhu, Y Zhang, L Chen, X Liu, L-H Zhang, Q-J Ye, B-S Li, J-Y Tang, A Ford, T Enver, F Liu, G-Q Chen, D-L Hong
The origin of cancers is associated with etiology as well as therapeutics. Several studies reveal that malignancies in children can originate in utero. However, a diagnostic approach to distinguish between cancers initiated pre- or postnatally is absent. Here we identified a transcriptional factor FEV (fifth Ewing variant) that was expressed in fetal hematopoietic cells and became silent after birth. We characterized that FEV was essential for the self-renewal of hematopoietic stem cells (HSCs). We next found that FEV was expressed in most infant leukemia samples, but seldom in adult samples, in accord with the known prenatal origins of the former...
December 9, 2016: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/27784215/epigenetic-effects-of-environmental-chemicals-on-reproductive-biology
#14
Maria Strazzullo, Maria Rosaria Matarazzo
Mammalian reproduction is a complex phenomenon. Human fertility is highly impacted from environmental exposure to toxicants as well as nutrients. The burden of environmental stimuli is heavy and multifaceted. The contaminant sources are many, often occult, and at present, the wide range of positive and negative consequences on the ecosystem and the human health is only partially understood. Compounds deriving from industrial manufacturing, pesticides, waste accumulation and burning are only some examples of the contaminants daily impacting human life...
October 25, 2016: Current Drug Targets
https://www.readbyqxmd.com/read/27783714/women-s-care-during-home-visits-for-the-first-comprehensive-healthcare-week
#15
Laura Lucinda Bezerra Silva, Katia Virginia Oliveira Feliciano, Lorena Novaes Ferraz Pacheco Oliveira, Evelyne Nascimento Pedrosa, Maria Suely Medeiros Corrêa, Ariani Impieri Souza
Objective: To evaluate women's care during home visits for the "First Comprehensive Care Week". Method: A cross-sectional study was carried out in a specialized service in Recife, Pernambuco, Brazil. A total of 190 women who had recently given birth enrolled at the Family Health Strategy answered the questionnaire between September and December 2013 to verify the association between home visits and the investigated variables. This association was verified using the chi-square test with a confidence level of 95%...
October 24, 2016: Revista Gaúcha de Enfermagem
https://www.readbyqxmd.com/read/27768967/the-impact-of-maternal-adiposity-specialization-on-infant-birthweight-upper-versus-lower-body-fat
#16
Alexandra C Sundermann, Troy D Abell, Lisa C Baker, Mark B Mengel, Kathryn E Reilly, Michael A Bonow, Gregory E Hoy, Richard D Clover
BACKGROUND: The specialization of human fat deposits is an inquiry of special importance in the study of fetal growth. It has been theorized that maternal lower-body fat is designated specifically for lactation and not for the growth of the fetus. OBJECTIVE: Our goal was to compare the contributions of maternal upper-body versus lower-body adiposity to infant birth weight. We hypothesized that upper-body adiposity would be strongly associated with infant birth weight and that lower-body adiposity would be weakly or negligibly associated with infant birth weight-after adjusting for known determinants...
November 2016: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/27759818/frequency-and-risk-factors-for-the-birth-of-small-for-gestational-age-newborns-in-a-public-maternity-hospital
#17
Marina Parca Cavelagna Teixeira, Tatiana Peloso Reis Queiroga, Maria Dos Anjos Mesquita
Objective: To determine the frequency and risk factors of small-for-gestational-age newborns in a high-risk maternity. Methods: This is an observational, cross-sectional, and case-control study, conducted in a public tertiary care maternity hospital. Data from 998 newborns and their mothers were collected through interviews and review of medical records and prenatal care cards. Some placentas underwent histopathological analysis. The variables of small-for-gestational-age and non-small-for-gestational-age newborns and of their mothers were statistically compared by means of Student's t test, Fisher's exact test, and odds ratio...
July 2016: Einstein
https://www.readbyqxmd.com/read/27729066/pre-natal-exposures-and-breast-tissue-composition-findings-from-a-british-pre-birth-cohort-of-young-women-and-a-systematic-review
#18
Rachel Denholm, Bianca De Stavola, John H Hipwell, Simon J Doran, Marta C Busana, Amanda Eng, Mona Jeffreys, Martin O Leach, David Hawkes, Isabel Dos Santos Silva
BACKGROUND: Breast density, the amount of fibroglandular tissue in the adult breast for a women's age and body mass index, is a strong biomarker of susceptibility to breast cancer, which may, like breast cancer risk itself, be influenced by events early in life. In the present study, we investigated the association between pre-natal exposures and breast tissue composition. METHODS: A sample of 500 young, nulliparous women (aged approximately 21 years) from a U...
October 12, 2016: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/27721317/analysis-of-the-relationship-between-the-month-of-birth-and-risk-of-multiple-sclerosis-in-a-spanish-population
#19
Rocío N Villar-Quiles, Jordi A Matías-Guiu, Gloria Ortega, Inés González-Suárez, Celia Oreja-Guevara, Jorge Matías-Guiu
BACKGROUND: Some studies have suggested an association between the month of birth and risk of multiple sclerosis (MS), related to environmental factors, mainly sun exposure and maternal vitamin D levels. Few studies have been conducted in Southern Europe countries. Madrid has a continental climate with considerable variation of sun hours between winter and summer, so it may be relevant to study this relationship. METHODS: MS patients, born between 1932 and 2003, 1,335 of them from our database between 2004 and 2015, were analysed...
2016: European Neurology
https://www.readbyqxmd.com/read/27687906/early-nutrition-growth-and-cognitive-development-of-infants-from-birth-to-2%C3%A2-years-in-malaysia-a-study-protocol
#20
Abdul Razak Nurliyana, Zalilah Mohd Shariff, Mohd Nasir Mohd Taib, Wan Ying Gan, Kit-Aun Tan
BACKGROUND: The first 2 years of life is a critical period of rapid growth and brain development. During this period, nutrition and environmental factors play important roles in growth and cognitive development of a child. This report describes the study protocol of early nutrition, growth and cognitive development of infants from birth to 2 years of age. METHODS/DESIGN: This is a prospective cohort study of mothers and infants recruited from government health clinics in Seremban district in Negeri Sembilan, Malaysia...
September 29, 2016: BMC Pediatrics
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