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c17.2 cell

Hojun Sung, Hyun Sik Kim, June-Young Lee, Woorim Kang, Pil Soo Kim, Dong-Wook Hyun, Euon Jung Tak, Mi-Ja Jung, Ji-Hyun Yun, Min-Soo Kim, Na-Ri Shin, Tae Woong Whon, Jeong Rae Rho, Sun Duk Park, Hyung Eun Shim, Jin-Woo Bae
A Gram-stain-positive, facultatively aerobic, spore-forming, oxidase-positive, catalase- and DNase-negative, rod-shaped and motile bacterial strain, AR23208T , was isolated from the gut of a cinereous vulture (Aegypius monachus), collected at Seoul Grand Park Zoo (Republic of Korea). Strain AR23208T grew optimally at 25-30 °C, at pH 7 and in the absence of NaCl. Phylogenetic analysis revealed that strain AR23208T shared 98.2 and 97.1 % 16S rRNA gene sequence similarity with Tumebacillus algifaecis THMBR28T and Tumebacilluslipolyticus NIO-S10T , respectively...
March 21, 2018: International Journal of Systematic and Evolutionary Microbiology
Chitti Thawai
A novel actinomycete, designated strain DH51B-4-3T , which formed long chains of spherical spores borne on the tip of sporophores arising from the aerial mycelium, was isolated from rice rhizosphere soil. The isolate contained meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars of strain DH51B-4-3T were arabinose, galactose, glucose, rhamnose and ribose. The phospholipids in the cell membrane were phosphatidylethanolamine, phosphatidyl methylethanolamine, diphosphatidylglycerol and phosphatidylinositol...
March 14, 2018: International Journal of Systematic and Evolutionary Microbiology
Peter Schumann, De-Chao Zhang, Luís França, Rosa Margesin
A Gram-stain-positive, flagellated, catalase- and cytochrome c oxidase-positive bacterial strain, designated S20-100T , was isolated from alpine forest soil. Growth occurred at a temperature range of 0-30 °C, at pH 6-9 and in the presence of 0-1 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain S20-100T was related to the genus Marmoricola and had the highest 16S rRNA gene sequence similarity to Marmoricola ginsengisoli Gsoil 097T (98.4 %) and Marmoricola solisilvae KIS18-7T (98...
March 2, 2018: International Journal of Systematic and Evolutionary Microbiology
Subhash Yadav, Radha Vaddavalli, Srinivas Siripuram, Ramaprasad Veera Venkata Eedara, Shivani Yadav, Ojha Rabishankar, Tushar Lodha, Sasikala Chintalapati, VenkataRamana Chintalapati
An antibiotic producing novel Planctomycete strain, designated JC280T , was isolated from the surface of the plant Hydrilla verticillata collected from an alkaline lake (Buffalo lake), University of Hyderabad, Hyderabad, India. The morphological and chemotaxonomic properties of strain JC280T were in agreement with the characteristics of the genus Planctopirus. The cell shape was spherical to ovoid and some were tear drop shaped. The cells were Gram-stain-negative divided by budding presenting stalks and rosette formation and were non-sporulating...
February 21, 2018: Journal of Antibiotics
Javier Pascual, Bärbel U Foesel, Alicia Geppert, Katharina J Huber, Christian Boedeker, Manja Luckner, Gerhard Wanner, Jörg Overmann
A novel slow-growing bacterium, designated strain AW1220T , was isolated from agricultural floodplain soil sampled at Mashare (Kavango region, Namibia) by using a high-throughput cultivation approach. Strain AW1220T was characterized as a Gram-negative, non-motile, rod-shaped bacterium. Occasionally, some cells attained an unusual length of up to 35 µm. The strain showed positive responses for catalase and cytochrome-c oxidase and divided by binary fission and/or budding. The strain had an aerobic chemoorganoheterotrophic metabolism and was also able to grow under micro-oxic conditions...
February 14, 2018: International Journal of Systematic and Evolutionary Microbiology
Zheng-Fei Yan, Pei Lin, Chang-Tian Li, MooChang Kook, Tae-Hoo Yi
A Gram-stain-positive, aerobic, non-motile and short-rod-shaped actinobacterium, designated THG-T121T , was isolated from forest soil. Growth occurred at 10-40 °C (optimum 28-30 °C), at pH 6-8 (optimum 7) and at 0-4 % NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T121T were identified as Actinotalea ferrariae KCTC 29134T (97.9 %), Actinotalea fermentans KCTC 3251T (97.3 %), Cellulomonas carbonis KCTC 19824T (97.2 %). 16S rRNA gene sequence similarities among strain THG-T121T and other recognized species were lower than 97...
January 17, 2018: International Journal of Systematic and Evolutionary Microbiology
Ming-Jing Shi, Chong Wang, Xu-Ting Wang, Zong-Jun Du
A novel Gram-stain-negative, strictly aerobic, non-flagellated and rod-shaped bacterium, designated HF004T , was isolated from a marine sediment sample collected from the coast of Weihai, China. The strain grew optimally at 28 °C, pH 7.5-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Based on the 16S rRNA gene sequence analysis, strain HF004T was a member of the genus Halioglobus, appearing to be closely related to Halioglobus pacificus (96.1 %) and Halioglobus japonicus (95.6 %). The major fatty acids were summed feature 3 (i...
January 29, 2018: International Journal of Systematic and Evolutionary Microbiology
Luciana Albuquerque, Ana Rita M Polónia, Cristina Barroso, Hugo J C Froufe, Olga Lage, Alexandre Lobo-da-Cunha, Conceição Egas, Milton S da Costa
An isolate, designated SPSPC-11T , with an optimum growth temperature of about 50 °C and an optimum pH for growth between 7.5 and 8.0, was recovered from a hot spring in central Portugal. Based on phylogenetic analysis of its 16S rRNA sequence, the new organism is most closely related to the species of the genus Thermonema but with a pairwise sequence similarity of <85 %. The isolate was orange-pigmented, formed non-motile long filaments and rod-shaped cells that stain Gram-negative. The organism was strictly aerobic, oxidase-positive and catalase-positive...
January 10, 2018: International Journal of Systematic and Evolutionary Microbiology
Kathrin I Mohr, Azam Moradi, Stefanie P Glaeser, Peter Kämpfer, Katja Gemperlein, Ulrich Nübel, Peter Schumann, Rolf Müller, Joachim Wink
An orange-coloured myxobacterium, MNa11734T , was isolated from desert in Iran. MNa11734T had rod-shaped vegetative cells, moved by gliding and was bacteriolytic. No real fruiting body formation could be observed, but sporangioles were produced on water agar. The strain was mesophilic, strictly aerobic and chemoheterotrophic. 16S rRNA gene analyses revealed that MNa11734T belonged to the family Nannocystaceae, genus Nannocystis and was closely related to Nannocystis pusilla Na p29T (DSM 14622T ) and Nannocystis exedens Na e1T (DSM 71T ), with 97...
January 18, 2018: International Journal of Systematic and Evolutionary Microbiology
Ram Hari Dahal, Jaisoo Kim
During a study of bacterial diversity of soil, a novel strain, CA-15T , was isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, motile, non-spore-forming, rod-shaped, oxidase-positive and catalase- negative. Tyrosine was not oxidized but produced red pigmentation on an agar palte. Strain CA-15T hydrolysed Tween 60 and DNA. It grew at 15-35 °C (optimum, 25-30 °C), pH 6.0-10.0 (optimum, 7.0-9.0) and at 1.5 % (w/v) NaCl concentration. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain CA-15T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria that was distinct from various species of the genus Brevundimonas...
January 15, 2018: International Journal of Systematic and Evolutionary Microbiology
Zubair Ahmed Nizamudeen, Lisa Chakrabarti, Virginie Sottile
Fasudil is a clinically approved Rho-associated protein kinase (ROCK) inhibitor that has been used widely to treat cerebral consequences of subarachnoid hemorrhage. It is known to have a positive effect on animal models of neurological disorders including Parkinson's disease and stroke. However, its cellular effect on progenitor populations and differentiation is not clearly understood. While recent studies suggest that fasudil promotes the mobilization of neural stem cells (NSCs) from the subventricular zone in vivo and promotes the differentiation of the C17...
February 6, 2018: Stem Cell Research
Lin Shi, Carl Brunius, Marko Lehtonen, Seppo Auriola, Ingvar A Bergdahl, Olov Rolandsson, Kati Hanhineva, Rikard Landberg
AIMS/HYPOTHESIS: The aims of the present work were to identify plasma metabolites that predict future type 2 diabetes, to investigate the changes in identified metabolites among individuals who later did or did not develop type 2 diabetes over time, and to assess the extent to which inclusion of predictive metabolites could improve risk prediction. METHODS: We established a nested case-control study within the Swedish prospective population-based Västerbotten Intervention Programme cohort...
January 18, 2018: Diabetologia
Lili Niu, Mengjie Xiong, Juan Zhang, Yangquan Xiang, Lei Song, Ziyi Hua, Wenying Li
A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterial strain, 7578-1T, was isolated from ripened Pu'er tea. Based on 16S rRNA gene sequence similarity comparisons, strain 7578-1T was grouped into the genus Bacillus and appeared to be closely related to the type strains Bacillus shackletoniiLMG 18435T (98.4 %), Bacillus acidicolaDSM 14745T (97.6 %), Bacillus paralicheniformis KACC 18426T (97.2 %) and Bacillus ginsengihumi KCTC 13944T (96.7 %). The fatty acid profile containing the major fatty acids, iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0 supported the allocation of strain 7578-1T to the genus Bacillus...
January 4, 2018: International Journal of Systematic and Evolutionary Microbiology
Dong-Shan An, Muhammad Zubair Siddiqi, Kyoung-Ho Kim, Hong-Shan Yu, Wan-Taek Im
A taxonomic study was conducted on BR7-21T, a bacterial strain isolated from the soil of a ginseng field in Baekdu Mountain. Comparative studies of the 16S rRNA gene sequence showed that the isolate was most closely related to Conexibacter woesei DSM 14684T, Solirubrobacter pauli ATCC BAA-492T, Patulibacter minatonensis JCM 12834T, with 93.8%, 92.4%, and 91.5% sequence similarity, respectively; each genus represented a family in the order Solirubrobacterales. Strain BR7-21T was Gram-reaction positive, non-spore forming, aerobic, non-motile, and short rod-shaped...
January 2018: Journal of Microbiology / the Microbiological Society of Korea
Yong-Guang Zhang, Xing-Kui Zhou, Jian-Wei Guo, Min Xiao, Hong-Fei Wang, Yun Wang, Khursheda Bobodzhanova, Wen-Jun Li
A Gram-stain-positive, alkaliphilic bacterium, designated EGI 80668T, was isolated from a Tamarix cone soil in Xinjiang, north-west China. Cells were facultatively anaerobic, terminal endospore-forming and motile by means of peritrichous flagella. Colonies were yellowish and the cells showed oxidase-negative and catalase-positive reactions. Strain EGI 80668T grew at pH 8.0-10.0 and with 0-10 % (w/v) NaCl (optimally at pH 9.0 and with 1-2 % NaCl) on marine agar 2216. The predominant menaquinone was MK-7...
January 3, 2018: International Journal of Systematic and Evolutionary Microbiology
Kristina Attoff, Anda Gliga, Jessica Lundqvist, Ulf Norinder, Anna Forsby
Despite its high relevance, developmental neurotoxicity (DNT) is one of the least studied forms of toxicity. Current guidelines for DNT testing are based on in vivo testing and they require extensive resources. Transcriptomic approaches using relevant in vitro models have been suggested as a useful tool for identifying possible DNT-generating compounds. In this study, we performed whole genome microarray analysis on the murine progenitor cell line C17.2 following 5 and 10 days of differentiation. We identified 30 genes that are strongly associated with neural differentiation...
2017: PloS One
René Maltais, Alexandre Trottier, Jenny Roy, Diana Ayan, Nicolas Bertrand, Donald Poirier
The development of a covalent inhibitor of 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1) is a promising approach for the treatment of hormone-dependent breast cancer and endometriosis. After reporting the steroid derivative PBRM as a first potent covalent inhibitor of 17β-HSD1 without estrogenic activity, we are now interested in studying its pharmaceutical behavior. The metabolism study in a human liver microsomal preparation showed a gradual transformation of PBRM into PBRM-O, an oxidized ketonic form of PBRM at position C17...
December 14, 2017: Journal of Steroid Biochemistry and Molecular Biology
Atsuko Ueki, Kazushi Goto, Nobuo Kaku, Katsuji Ueki
A strictly anaerobic bacterial strain (FH042T ) was isolated from a methanogenic reactor treating waste from cattle farms. Cells were stained Gram-positive, straight to gently curved rods with polar flagella. The strain was asaccharolytic. The strain fermented amino acids (l-arginine, l-lysine and l-serine) as growth substrates and produced acetate and butyrate. The optimum temperature for growth was 30 °C and the optimum pH was 6.1-6.8. Oxidase, catalase and nitrate-reducing activities were negative. Hydrogen sulfide was produced...
January 2018: International Journal of Systematic and Evolutionary Microbiology
Bo Zhang, Zhi-Qiang Liu, Yu-Guo Zheng
A Gram-stain-negative, aerobic, rod-shaped, bacterium, C62-2T , was isolated from activated sludge in Fujian Province, China. Phylogenetic analysis of the 16S rRNA gene sequences showed that it was closely related to Pedobacter duraquae WB 2.1-25T (97.92 %), Pedobacter bambusae THG-G118T (97.40 %), Pedobacter cryoconitis A37T (97.37 %) and Pedobacter caeni LMG 22862T (97.3 %). Cells grew aerobically at 20-37 °C (optimum, 30 °C), pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-3.0 % (w/v) NaCl...
January 2018: International Journal of Systematic and Evolutionary Microbiology
Alvaro S Villalobos, Jutta Wiese, Pablo Aguilar, Cristina Dorador, Johannes F Imhoff
A novel actinobacterium, strain DB165T , was isolated from cold waters of Llullaillaco Volcano Lake (6170 m asl) in Chile. Phylogenetic analysis based on 16S rRNA gene sequences identified strain DB165T as belonging to the genus Subtercola in the family Microbacteriaceae, sharing 97.4% of sequence similarity with Subtercola frigoramans DSM 13057T , 96.7% with Subtercola lobariae DSM 103962T , and 96.1% with Subtercola boreus DSM 13056T . The cells were observed to be Gram-positive, form rods with irregular morphology, and to grow best at 10-15 °C, pH 7 and in the absence of NaCl...
December 6, 2017: Antonie Van Leeuwenhoek
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