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c17.2 cell

Der-Shyan Sheu, Shih-Yi Sheu, Pei-Bei Xie, Sen-Lin Tang, Wen-Ming Chen
Strain Eup a-8(T), isolated from a torch coral Euphyllia glabrescens, was characterized using a polyphasic taxonomy approach. Cells of strain Eup a-8(T) were Gram-staining-negative, aerobic, motile by means of a single polar flagellum, poly-β-hydroxybutyrate-containing, rod-shaped and formed white colonies. Optimal growth occurred at 25-30 °C, pH 7-8, and in the presence of 2 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Eup a-8(T) belonged to the genus Thalassotalea and showed the highest levels of sequence similarity with respect to Thalassotalea ganghwensis JC2041(T) (97...
November 16, 2017: International Journal of Systematic and Evolutionary Microbiology
Le-Ni Sun, Dan-Dan Pan, Xiang-Wei Wu, En-Dong Yang, Ri-Mao Hua, Qing X Li
A Gram-stain-positive, rod-shaped, non-motile bacterial strain, designated JW-1(T), was isolated from activated sludge collected from the outlet of an aeration tank in a prometryn-manufacturing plant, located in Binzhou City, Shandong province, PR China. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain JW-1(T) belongs to the genus Leucobacter and its closest neighbours are 'Leucobacter kyeonggiensis' F3-P9 (98.95 % similarity), Leucobacter celer subsp. astrifaciens CBX151(T) (98...
November 15, 2017: International Journal of Systematic and Evolutionary Microbiology
Jisun Park, Min-Kyeong Kim, Bo-Ram Yun, Ji-Hye Han, Seung Bum Kim
A Gram-stain-positive strain, designated DT7-02(T), was isolated from the surface-sterilized root of Oenotherabiennis (evening primrose) and subjected to taxonomic characterization. Cells of DT7-02(T) were slender rod-shaped, motile by means of flagella, and oxidase- and catalase-positive. The colonies were circular, pinkish-yellow, opaque, glistering and 1-2 mm in diameter. The strain was moderately thermophilic and halophilic, as growth occurred at 20-44 °C (optimum 40 °C), pH 7-10 (optimum pH 8-9) and in the presence of 0-8 % of NaCl (optimum 4 %) in tryptic soy broth...
November 10, 2017: International Journal of Systematic and Evolutionary Microbiology
Zi-Wen Yang, Nimaichand Salam, Mohamed Mohany, Arunachalam Chinnathambi, Sulaiman Ali Alharbi, Min Xiao, Wael N Hozzein, Wen-Jun Li
Strains SYSU D8007(T) and SYSU D8014(T) were isolated from desert soil collected from Saudi Arabia. The two isolates were Gram-stain-positive, non-motile, aerobic and non-spore-forming. These strains were able to grow at 4-45 °C and in the presence of up to 8 % (w/v) NaCl. Strain SYSU D8007(T) could grow at pH 6.0-10.0, and strain SYSU D8014(T) at pH 5.0-10.0. They shared highest 16S rRNA gene sequence similarities with Microbacterium marinilacus YM11-607(T) and Microbacterium paludicola US15(T). Menaquinones MK-11 and MK-12 were detected as the respiratory quinones...
November 10, 2017: International Journal of Systematic and Evolutionary Microbiology
Yu-Mei Dai, Ling-Li Zhang, Yan Li, Yu-Qian Li, Xiao-Hui Deng, Ting-Ting Wang, Feng Yang, Yong-Qiang Tian, Na Li, Xing-Mei Zhou, Xiao-Feng Liu, Wen-Jun Li
Two psychrotolerant facultative anaerobes, strains B7-2(T) and B5(T), were isolated from the Zoige Wetland on the Qinghai-Tibetan Plateau. The 16S rRNA gene sequences of strains B7-2(T) and B5(T) shared high similarity (>99 %) with those of the type strains of the genus Trichococcus, while their digital DNA-DNA hybridization values with each other (49 %) and with the reference type strains (48-23 %) were lower than 70 %, which suggest that they represent two novel species of the genus Trichococcus...
November 8, 2017: International Journal of Systematic and Evolutionary Microbiology
Shih-Yao Lin, Asif Hameed, Yu-Ting Hsieh, Yi-Han Hsu, Wei-An Lai, Chiu-Chung Young
A polyphasic taxonomic approach was used to characterize a presumably novel bacterium, designated strain CC-YTH191(T), isolated from a fermented meal in Taiwan. Cells of strain CC-YTH191(T) were Gram-stain-negative aerobic rods, which grew at 15-40 °C (optimal 25-30 °C), pH 6.0-9.0 (optimal 7.0) and 1-2 % (w/v) NaCl (optimal 1 %). On the basis of 16S rRNA gene sequence analysis, strain CC-YTH191(T) appeared to belong to the genus Castellaniella, and was closely related to Castellaniella hirudinis (96...
November 8, 2017: International Journal of Systematic and Evolutionary Microbiology
Bo Liu, Guo-Hong Liu, Xiao-Ying Wang, Jie-Ping Wang, Yu-Jing Zhu, Hai-Feng Zhang, Cetin Sengonca
A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-45347(T), was isolated from rhizosphere soil collected from the Taklamakan desert in Xinjiang (PR China). Growth was observed at 15-35 °C (optimum 25 °C), in 0 % and 20.0 % NaCl (optimum 8.0 %) and at pH 7.5-12.0 (optimum 8.0), respectively. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7. The main fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine...
November 7, 2017: International Journal of Systematic and Evolutionary Microbiology
Hao Wu, Bin Liu, Xiaoyun Ou, Shangli Pan, Yuanyuan Shao, Fuchang Huang
During an investigation exploring potential sources of novel thermophilic species and natural products, a novel thermophilic and alkaliphilic actinomycete with alkaline cellulase producing ability, designated strain 4-2-13(T), was isolated from soil of a tropical rainforest in Xishuangbanna, Yunnan province, China. The morphological and chemotaxonomic characteristics of strain 4-2-13(T) are consistent with those of the members of the genus Streptomyces. The strain forms extensively branched aerial mycelia and substrate mycelia...
November 7, 2017: Antonie Van Leeuwenhoek
Marilize le Roes-Hill, Alaric Prins, Paul R Meyers
As part of an antibiotic screening program, an actinobacterium, strain HMC13(T), was isolated from soil collected from the banks of the Gamka River, Western Cape Province, South Africa. The isolate was found to produce branched mycelia that differentiated into spiral spore chains with spiny spores. 16S rRNA gene sequence analysis showed the strain to be closely related to Streptomyces caelestis NRRL 2418(T) (99.72%) and Streptomyces azureus NBRC 12744(T) (99.51%). Chemotaxonomic analyses confirmed the classification of the strain as a member of the genus Streptomyces: LL-DAP in the peptidoglycan, no diagnostic sugars in the whole cell sugar pattern, dominant menaquinones including MK9(H8), MK9(H6), and the polar lipids detected included phosphatidylethanolamine...
November 6, 2017: Antonie Van Leeuwenhoek
Yang Zhao, Daoyin Dong, E Albert Reece, Ashley R Wang, Peixin Yang
BACKGROUND: Maternal diabetes induces neural tube defects, and oxidative stress is a causal factor for maternal diabetes-induced neural tube defects. The redox gene nuclear factor erythroid 2-related factor 2 is the master regulator of the cellular antioxidant system. OBJECTIVE: In this study, we aimed to determine whether maternal diabetes inhibits nuclear factor erythroid 2-related factor 2 expression and nuclear factor erythroid 2-related factor 2-controlled antioxidant genes through the redox-sensitive miR-27a...
November 1, 2017: American Journal of Obstetrics and Gynecology
Gabriela Moya, Zheng-Fei Yan, Dong-Hun Chu, KyungHwa Won, Jung-Eun Yang, Qi-Jun Wang, Moo-Chang Kook, Tae-Hoo Yi
A Gram-stain-positive, pink-pigmented, coccus-shaped, strictly aerobic, non-motile bacterium, strain THG-AG1.5(T), was isolated from rhizosphere of Hibiscus syriacus L. (Mugunghwa flower) located in Kyung Hee University, Yongin, Gyeonggi, Republic of Korea. The isolated strain grew optimally at 25-30 °C, at pH 6.0-7.5 and in the presence of additional 0-1.5 % (w/v) NaCl. Strain THG-AG1.5(T) exhibited tolerance to UV radiation (>1500 J m(-2)) and to gamma radiation (>12 kGy). Based on 16S rRNA gene sequence comparisons, strain THG-AG1...
November 2, 2017: International Journal of Systematic and Evolutionary Microbiology
Bora Shin, So Hyun Park, Byung-Yong Kim, Shin-Il Jo, Sang Kook Lee, Jongheon Shin, Dong-Chan Oh
Four new aminoglycolipids, deinococcucins A-D (1-4), were discovered from a Deinococcus sp. strain isolated from the gut of queen carpenter ants, Camponotus japonicus. The structures of deinococcucins A-D were elucidated as a combination of N-acetyl glucosamine, 2,3-dihydroxypropanoic acid, and an alkyl amine with a C16 or C17 hydrocarbon chain primarily based on 1D and 2D NMR and mass spectroscopic data. The exact location of the olefinic double bond in deinococcucins C and D (3 and 4) was assigned based on the liquid chromatography-mass spectroscopy data obtained after olefin metathesis...
November 1, 2017: Journal of Natural Products
Juan Li, Chisaki Kudo, Akio Tonouchi
A bacterial strain designated PtRA-8(T) was isolated from the trunk surface of a Japanese aspen tree (Populus tremula var. sieboldii). Cells of strain PtRA-8(T) were aerobic, non-motile, non-spore forming, Gram-stain-negative rods, 1.0‒2.0 µm in width and 3.0‒10.0 µm in length. The pH range for growth was between 5.5 and 7.5, with an optimum at 6.5. The temperature range for growth was between 10 and 37 °C, with an optimum at around 25‒30 °C. Strain PtRA-8(T) was highly resistant to UV irradiation, similar to its Deinococcus relatives...
October 28, 2017: Archives of Microbiology
Shih-Yi Sheu, Ching-Ling Su, Soon-Wo Kwon, Wen-Ming Chen
Strain KYPY10(T), isolated from a water sample taken from the Funglin Stream in Taiwan, was characterized using a polyphasic taxonomic approach. Cells of strain KYPY10(T) were Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped surrounded by a thick capsule, and formed light-yellow colonies. Growth occurred at 20-30 °C (optimum, 25 °C), at pH 6-7 (optimum, pH 6) and with 0-0.2 % NaCl (optimum, 0 % w/v). Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strain KYPY10(T) belonged to the genus Flavobacterium and was most closely related to Flavobacterium brevivitae TTM-43(T) (98...
October 26, 2017: International Journal of Systematic and Evolutionary Microbiology
Ram Hari Dahal, Dhiraj Kumar Chaudhary, Jaisoo Kim
Two strains of bacteria, designated J107-1(T) and J107-2, were isolated from farmland soil. Cells were aerobic, Gram-stain-negative, catalase- and oxidase-positive, non-motile, non-spore-forming, coccoid rod-shaped and yellow-pigmented. Flexirubin-type pigments were absent. Both strains hydrolysed casein and tyrosine. Strains could grow at pH 9.0 and at 42 °C. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strains J107-1(T) and J107-2 formed a lineage within the phylum Bacteroidetes that was distinct from various members of the family Chitinophagaceae, including Ferruginibacter paludis HME8881(T) (89...
October 26, 2017: International Journal of Systematic and Evolutionary Microbiology
Decai Jin, Xiao Kong, Minghong Jia, Xiangyang Yu, Xinxin Wang, Xuliang Zhuang, Ye Deng, Zhihui Bai
A phthalate esters-degrading bacterial strain, designated QH-11(T), was isolated from an activated sludge wastewater treatment plant in Beijing, PR China. The cells were aerobic, Gram-stain-positive, non-motile, catalase-positive, oxidase-negative, short rods and formed white colonies on trypticase soy agar. This isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and whole-cell hydrolysates contained arabinose and ribose. Diphosphatidylglycerol and phosphatidylethanolamine were the predominant polar lipids...
October 25, 2017: International Journal of Systematic and Evolutionary Microbiology
Lingfei Lu, Min Cao, Dan Wang, Kai Yuan, Weiping Zhuang, Wei Guo, Gejiao Wang
A Gram-positive, non-motile, rod-shaped aerobic bacterial strain FLL521(T) was isolated from iron mine soil. Phylogenetic trees based on 16S rRNA gene sequences showed that strain FLL521(T) belonged to genus Nocardioides in family Nocardioidaceae, with the highest sequence identity to Nocardioides panacisoli GSoil 346(T) (96.3 %). The DNA G+C content of strain FLL521(T) was 70.3 mol%. The cell-wall peptidoglycan type was ll-2,6-diaminopimelic acid. The major respiratory quinone was MK-8(H4), and the predominant fatty acids (>5 %) were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c...
October 25, 2017: International Journal of Systematic and Evolutionary Microbiology
Rui Zou, Yumin Zhang, Xueyin Zhou, Yang Wang, Fang Peng
A yellow-pigmented strain, designated Y4AR-5(T), was characterized by using a polyphasic approach. The strain was isolated from a tundra soil from near Longyearbyen, Svalbard Islands, Norway. The cells were Gram-stain-negative, aerobic, rod-shaped and non-motile. Growth occurred at 4-28 °C (optimum 20 °C) and pH 6.0-9.0 (optimum pH 8.0) and with 0-0.5 % (w/v) NaCl (optimum 0 %). The major respiratory quinone was MK-7. The polar lipids were phosphatidylethanolamine (PE), an aminophospholipid (APL), a phospholipid (PL), an unidentified aminolipid (AL) and two unidentified lipids...
October 23, 2017: International Journal of Systematic and Evolutionary Microbiology
Jingran Huang, Zhaobin Huang, Zongze Shao
Two strains, AFRC7-2-1(T) and CFD7-4-2, were isolated from the intestine of abalone, Haliotis discus hannai. The bacterial cells were Gram-stain-negative, stick-shaped and non-flagellated. They were facultative anaerobic bacteria and could reduce Fe(III) to Fe(II). The 16S rRNA genes of two strains shared 99.7 % sequence similarity, and had the highest similarity of 97.3-97.5 % with Paraferrimonas sedimenticola NBRC 101628(T), and of <94.0 % with other species. Phylogenetic relationship showed that the two strains formed a tight clade with an isolate Paraferrimonas sp...
October 17, 2017: International Journal of Systematic and Evolutionary Microbiology
Ramya R, Manjari Shrivastava, Namitha N N, Ram Lal Thakur, Arunaloke Chakrabarti, Utpal Roy
New lipopeptide homologues (AF3, AF4 and AF5) with antifungal activities against Candida and Cryptococcus spp. were purified from the cell free supernatant of Bacillus subtilis RLID 12.1. The lipopeptides AF3, AF4 and AF5 were identified with the same peptide sequence Asn-Pro-Tyr-Asn-Gln-Thr-Ser with variations in the fatty acid branching type and chain length (anteiso-C17, iso-C17 and iso-C18 respectively). Comparing the three homologues for minimum inhibitory concentrations (MIC) against 81 Candida (64 no...
October 16, 2017: Antimicrobial Agents and Chemotherapy
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