keyword
https://read.qxmd.com/read/38506089/retinoic-acid-induced-specific-changes-in-the-phosphoproteome-of-c17-2-neural-stem-cells
#1
JOURNAL ARTICLE
Cheng Zhang, Lite Ge, Huali Xie, Xiaoqian Liu, Chengfeng Xun, Yan Chen, Haiyan Chen, Ming Lu, Ping Chen
Retinoic acid (RA), a vitamin A derivative, is an effective cell differentiating factor which plays critical roles in neuronal differentiation induction and the production of neurotransmitters in neurons. However, the specific changes in phosphorylation levels and downstream signalling pathways associated with RA remain unclear. This study employed qualitative and quantitative phosphoproteomics approaches based on mass spectrometry to investigate the phosphorylation changes induced by RA in C17.2 neural stem cells (NSCs)...
April 2024: Journal of Cellular and Molecular Medicine
https://read.qxmd.com/read/38489089/maribacter-halichondriae-sp-nov-isolated-from-the-marine-sponge-halichondria-panicea-displays-features-of-a-sponge-associated-life-style
#2
JOURNAL ARTICLE
Leon X Steiner, Jutta Wiese, Tanja Rahn, Erik Borchert, Beate M Slaby, Ute Hentschel
A new member of the family Flavobacteriaceae (termed Hal144T ) was isolated from the marine breadcrumb sponge Halichondria panicea. Sponge material was collected in 2018 at Schilksee which is located in the Kiel Fjord (Baltic Sea, Germany). Phylogenetic analysis of the full-length Hal144T 16S rRNA gene sequence revealed similarities from 94.3 to 96.6% to the nearest type strains of the genus Maribacter. The phylogenetic tree of the 16S rRNA gene sequences depicted a cluster of strain Hal144T with its closest relatives Maribacter aestuarii GY20T (96...
March 15, 2024: Antonie Van Leeuwenhoek
https://read.qxmd.com/read/38460344/stomatohabitans-albus-gen-nov-sp-nov-an-oral-living-facultative-anaerobic-actinobacteria-isolated-form-steller-sea-lion-and-proposal-of-stomatohabitantaceae-fam-nov-and-stomatohabitantales-ord-nov
#3
JOURNAL ARTICLE
Zi-Wen Yang, Wen-Li Liu, Peng Zhang, Dan-Yuan Guo, Hong-Chuan Wang, Jia-Ling Li, Pan-Deng Wang, Gui-Xin Dong, Guo-Xing Nie, Wen-Jun Li
Two novel actinobacteria, designated as SYSU M7M538T and SYSU M7M531, were isolated from oral of Eumetopias jubatus in Zhuhai Chimelong Ocean Kingdom, China. The cells of these microorganisms stained Gram-positive and were rod shaped. These strains were facultative anaerobic, and catalase-positive. Optimal growth occurred at 37 °C and pH 7.0 over 7 days of cultivation. Both strains possessed diphosphatidylglycerol, phosphatidylglycerol and phosphocholine as the major polar lipids. The main menaquinone was MK-9(H4 )...
March 7, 2024: Systematic and Applied Microbiology
https://read.qxmd.com/read/38446393/saxibacter-everestensis-gen-nov-sp-nov-a-novel-member-of-the-family-brevibacteriaceae-isolated-from-the-north-slope-of-mount-everest
#4
JOURNAL ARTICLE
Mao Tian, Shiyu Wu, Wei Zhang, Gaosen Zhang, Xue Yu, Yujie Wu, Puchao Jia, Binglin Zhang, Tuo Chen, Guangxiu Liu
We isolated and analyzed a novel, Gram-stain-positive, aerobic, rod-shaped, non-motile actinobacterium, designated as strain ZFBP1038T , from rock sampled on the north slope of Mount Everest. The growth requirements of this strain were 10-37 °C, pH 4-10, and 0-6% (w/v) NaCl. The sole respiratory quinone was MK-9, and the major fatty acids were anteiso-C15:0 and iso-C17:0 . Peptidoglycan containing meso-diaminopimelic acid, ribose, and glucose were the major cell wall sugars, while polar lipids included diphosphatidyl glycerol, phosphatidyl glycerol, an unidentified phospholipid, and an unidentified glycolipid...
March 6, 2024: Journal of Microbiology / the Microbiological Society of Korea
https://read.qxmd.com/read/38382327/panax-notoginseng-saponins-stimulates-the-differentiation-and-neurite-development-of-c17-2-neural-stem-cells-against-ogd-r-injuries-via-mtor-signaling
#5
JOURNAL ARTICLE
Jiale Gao, Mingjiang Yao, Yehao Zhang, Yunyao Jiang, Jianxun Liu
Ischemic stroke remains a major disease worldwide, and most stroke patients often suffer from serious sequelae. Endogenous neurogenesis matters in the repair and regeneration of impaired neural cells after stroke. We have previously reported in vivo that PNS could strengthen the proliferation and differentiation of neural stem cells (NSCs), modulate synaptic plasticity and protect against ischemic brain injuries in cerebral ischemia rats, which could be attributed to mTOR signaling activation. Next, to obtain further insights into the function mechanism of PNS, we evaluated the direct influence of PNS on the survival, differentiation and synaptic development of C17...
February 20, 2024: Biomedicine & Pharmacotherapy
https://read.qxmd.com/read/38369673/nesterenkonia-marinintestina-sp-nov-isolated-from-the-fish-intestine
#6
JOURNAL ARTICLE
Aolin Zhao, Hanqin Cai, Ying Huang, Quan Yang, Zhenxin Zhu, Yan Zhou, Mingguo Jiang, Yi Jiang, Wenshan Huang
A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115T , was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0-3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4-45 °C (optimum 30 °C), at pH 4.5-10.0 (optimum pH 7...
February 19, 2024: Archives of Microbiology
https://read.qxmd.com/read/38329394/-glycomyces-niveus-sp-nov-a-novel-actinomycete-isolated-from-sandy-soil
#7
JOURNAL ARTICLE
Xilang Yang, Hanshui Cao, Xi Xu, Jiayue Xie, Jie Chen, Wensheng Xiang, Junwei Zhao, Xiangjing Wang
A novel mycelium-forming actinomycete, designated strain NEAU-S30T , was isolated from the sandy soil of a sea beach in Shouguang city, Shandong province, PR China. The strain developed long chains of non-motile cylindrical spores with smooth surfaces on aerial mycelia. The results of a polyphasic taxonomic study indicated that NEAU-S30T represented a member of the genus Glycomyces . The results of 16S rRNA gene sequence analysis indicated that NEAU-S30T was closely related to 'Glycomyces luteolus ' (98.97 % sequence similarity), Glycomyces algeriensis (98...
February 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38323900/three-fe-iii-reducing-and-nitrogen-fixing-bacteria-anaeromyxobacter-terrae-sp-nov-anaeromyxobacter-oryzisoli-sp-nov-and-anaeromyxobacter-soli-sp-nov-isolated-from-paddy-soil
#8
JOURNAL ARTICLE
Rong Tang, Shang Yang, Manik Prabhu Narsing Rao, Cheng-Jie Xie, Shuang Han, Qiu-E Yang, Christopher Rensing, Guo-Hong Liu, Yong Yuan, Shun-Gui Zhou
Three microaerophilic bacterial strains, designated SG22T , SG63T and SG29T were isolated from paddy soils in PR China. Cells of these strains were Gram-staining-negative and long rod-shaped. SG22T , SG63T and SG29T showed the highest 16S rRNA gene sequence similarities with the members of the genus Anaeromyxobacter . The results of phylogenetic and phylogenomic analysis also indicated that these strains clustered with members of the genus Anaeromyxobacter . The main respiratory menaquinone of SG22T , SG63T and SG29T was MK-8 and the major fatty acids were iso-C15 : 0 , iso-C17 : 0 and C16 : 0 ...
February 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38315525/-streptomyces-herbicida-sp-nov-a-novel-actinomycete-with-antibacterial-and-herbicidal-activity-isolated-from-soil
#9
JOURNAL ARTICLE
Yanfang Luo, Ling Ling, Kun Zhang, Zhengke Song, Lida Zhang, Jiaxin Li, Jie Chen, Wensheng Xiang, Xiangjing Wang, Junwei Zhao
A novel actinobacterial strain (NEAU-HV9T ) showing antibacterial activity against Ralstonia solanacearum and herbicidal activity against Amaranthus retroflexus L. was isolated from soil sampled in Bama yao Autonomous County, Hechi City, Guangxi Zhuang Autonomous Region. The strain is aerobic and Gram-positive. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-HV9T belonged to the genus Streptomyces and showed high 16S rRNA sequence similarity to Streptomyces panaciradicis 1MR-8T (98...
February 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38271832/the-serine-protease-2-gene-regulates-lipid-metabolism-through-the-lep-ampk%C3%AE-1-srebp1-pathway-in-bovine-mammary-epithelial-cells
#10
JOURNAL ARTICLE
Huixian Lu, Zhihui Zhao, Haibin Yu, Ambreen Iqbal, Ping Jiang
Molecular breeding has brought about significant transformations in the milk market and production system during the twenty-first century. The primary economic characteristic of dairy production pertains to milk fat content. Our previous transcriptome analyses revealed that serine protease 2 (PRSS2) is a candidate gene that could impact milk fat synthesis in bovine mammary epithelial cells (BMECs) of Chinese Holstein dairy cows. To elucidate the function of the PRSS2 gene in milk fat synthesis, we constructed vectors for PRSS2 overexpression and interference and assessed intracellular triglycerides (TGs), cholesterol (CHOL), and nonesterified fatty acid (NEFA) contents in BMECs...
January 21, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38190227/-actinoplanes-oblitus-sp-nov-producing-the-glycopeptide-antibiotic-a477
#11
JOURNAL ARTICLE
Oleksandr Yushchuk, Elisa Binda, Christian Rückert-Reed, Francesca Berini, Victor Fedorenko, Jörn Kalinowski, Flavia Marinelli
In 1973, Eli Lilly and Company described the filamentous actinomycete producing the glycopeptide antibiotic A477 as an Actinoplanes species on the basis of its morphological and physiological features and deposited it as NRRL 3884T . In this paper, we report that the phylogenetic analysis based on the 16S rRNA gene sequence and the whole genome phylogenomic study indicate that NRRL 3884T forms a distinct monophyletic line within the genus Actinoplanes , being most closely related to Actinoplanes octamycinicus NBRC 14524T [99...
January 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38189401/-sulforaphane-reverses-a%C3%AE-fiber-mediated-m1-type-microglia-polarization-and-neuroinflammation-mediated-necroptosis-of-neural-stem-cells-by-downregulating-the-mapk-nf-%C3%AE%C2%BAb-signaling-pathways
#12
JOURNAL ARTICLE
J Zhang, C Yang, S Zhang, T Cao, R Peng, W Guo, Y Yan, S Xie, X Peng, T Lü, T Huang
OBJECTIVE: To explore the effects of sulforaphane (SFN) and Aβ25-35 fibers (fAβ25-35 ) on M1/M2 polarization of BV-2 cells and neuroinflammation-mediated programmed necrosis of neural stem cells. METHODS: BV-2 cells treated with different concentrations of fAβ25-35 and SFN were examined for changes in cell viability using the CCK-8 kit. The effect of fAβ25-35 alone or in combination with SFN or SB203580 on expressions of IL-6 and TNF- α mRNA and proteins were assessed in BV-2 cells using RT-qPCR and ELISA...
December 20, 2023: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://read.qxmd.com/read/38189368/-microbacterium-memoriense-sp-nov-a-member-of-the-actinomycetota-from-marine-beach-sediment-of-the-north-coast-of-portugal
#13
JOURNAL ARTICLE
José Diogo Neves Dos Santos, Dominika Klimek, Magdalena Calusinska, Alexandre Lobo-da-Cunha, José Catita, Hugo Gonçalves, Ignacio González, Olga Maria Lage
The oceans harbour a myriad of unknown micro-organisms that remain unstudied because of a failure to establish the right growth conditions under laboratory conditions. To overcome this limitation, an isolation effort inspired by the iChip was performed using marine sediments from Memória beach, Portugal. A novel strain, PMIC_1C1BT , was obtained and subjected to a polyphasic study. Cells of strain PMIC_1C1BT were Gram-positive, rod-shaped, divided by binary fission and formed colonies that were shiny light-yellow...
January 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38180333/-actinoplanes-pyxinae-sp-nov-a-new-lichen-derived-rare-actinobacterium-exhibiting-antimicrobial-and-anticancer-activity
#14
JOURNAL ARTICLE
Achiraya Somphong, Trinset Weeraphan, Vasun Poengsungnoen, Chanwit Suriyachadkun, Paranee Sripreechasak, Chatchai Chaotham, Somboon Tanasupawat, Wongsakorn Phongsopitanun
A novel lichen-derived actinobacterium, designated Pm04-4T , was isolated from Pyxine cocoes (Sw.) Nyl. lichen collected from Chaiyaphum, Thailand. A polyphasic approach was used to describe the taxonomic position of the strain. The strain had morphological and chemotaxonomic properties similar to members of the genus Actinoplanes . It produced sporangia on the substrate mycelia. Meso -diaminopimelic acid, galactose, glucose and mannose were detected in the whole-cell hydrolysate of the strain. The major menaquinone was MK-9(H4 )...
January 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38180325/-actinoplanes-sandaracinus-sp-nov-a-novel-ligninase-producing-and-cellulose-degrading-actinobacterium-isolated-from-soil
#15
JOURNAL ARTICLE
Zishan Wang, Yan Xu, Changjian Zhou, Xiujun Sun, Zhenzhen Huang, Chuan He, Siqi Yao, Junwei Zhao, Xiangjing Wang, Jia Song, Wensheng Xiang
A novel ligninase-producing and cellulose-degrading actinobacterium, designated strain NEAU-A12T , was isolated from a soil sample collected from Aohan banner, Chifeng City, Inner Mongolia Autonomous Region, PR China. A polyphasic taxonomic study was used to establish the status of strain NEAU-A12T . 16S rRNA gene sequence analysis revealed that strain NEAU-A12T belonged to the genus Actinoplanes and showed the highest similarity (98.3 %) to Actinoplanes palleronii DSM 43940T , while showing less than 98...
January 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38079210/-marivirga-aurantiaca-sp-nov-a-halophilic-nitrite-reducing-bacterium-isolated-from-intertidal-surface-sediments
#16
JOURNAL ARTICLE
Mingxia Zhang, Yulian Zhang, Qing Yao, Fan Yang, Honghui Zhu
A novel Gram-stain-negative strain, designated S37H4T , was isolated from an intertidal surface sediment sample collected from Zhanjiang City, Guangdong province, south PR China. Cells of the strain were aerobic, non-flagellated, long rod-shaped and motile by gliding. S37H4T could grow at 4-40 °C, pH 7.0-8.5 and in 2.0-15.0 % NaCl, with optimal growth at 25-30 °C, pH 7.5 and 9.0 % NaCl, respectively. S37H4T was capable of nitrite removal under high-salt conditions, and there were three denitrification genes, nirK , norB and nosZ, in its genome...
December 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38054484/-flavobacterium-sedimenticola-sp-nov-isolated-from-sediment
#17
JOURNAL ARTICLE
Ningning Wu, Le Liu, Xueting Jiang, Ye Yuan, Dongmei Mao, Jiahui Shao, Jian He, Qirong Shen
A novel yellow-pigmented bacterial strain, designated YZ-48T , was isolated from the sediment of the Yangtze River, PR China. Cells were Gram-stain-negative, non-motile, rod-shaped, strictly aerobic, catalase-positive and oxidase-positive. The strain grew optimally on R2A medium at 37 °C, pH 7.0 and with 1.0 % (w/v) NaCl. Strain YZ-48T showed the closest 16S rRNA gene sequence similarity to Flavobacterium solisilvae SE-s27T (96.4 %) and F. dankookense DSM 25687T (96.2 %). The phylogenetic trees based on 16S rRNA gene sequences showed that strain YZ-48T belonged to the genus Flavobacterium but formed a distinct phylogenetic lineage...
December 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37966456/-robiginitalea-aestuariiviva-sp-nov-isolated-from-sediment-of-tidal-flat-located-in-zhejiang-pr-china
#18
JOURNAL ARTICLE
Ke Cao, Jia-Wei Gao, Wen-Wu Zhang, Yu-Ruo Wang, Yue Su, Jun-Jie Ying, Lin Xu, Yan Bai, Cong Sun
A Gram-stain-negative, strictly aerobic and rod- to coccoid-shaped bacterium, designated as strain M366T , was isolated from coastal sediment of Jiaoshanjiao, Zhejiang Province, PR China (121°54' E 29 °38' N). The draft genome of strain M366T was 3 225 479 bp long (with 55.6 mol% G+C content) and assembled into four contigs. The N50 value was 563 270 bp and the genomic completeness and contamination were estimated to be 99.34 and 0.05 %, respectively...
November 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37962187/-neopusillimonas-aromaticivorans-sp-nov-isolated-from-poultry-manure
#19
JOURNAL ARTICLE
Shih-Yao Lin, Tzu-Yu Lin, Asif Hameed, Yu-Shan Tang, Chiu-Chung Young
A polyphasic taxonomic approach was used to characterize a novel bacterium, designated strain CC-YST667T , isolated from poultry manure sampled in Taiwan. The cells were observed to be aerobic, motile and non-spore-forming rods, displaying positive reactions for oxidase. Optimal growth of CC-YST667T was observed at 25 °C, pH 8.0 and with 1 % (w/v) NaCl. The polar lipid profile consisted of phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and multiple unidentified polar lipids...
November 2023: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/37952730/periplaneta-americana-l-extract-activates-the-erk-creb-bdnf-pathway-to-promote-post-stroke-neuroregeneration-and-recovery-of-neurological-functions-in-rats
#20
JOURNAL ARTICLE
Jiangyan Rao, Hongpu Li, Haonan Zhang, Xiaoxia Xiang, Xinyu Ding, Li Li, Funeng Geng, Hongyi Qi
ETHNOPHARMACOLOGICAL RELEVANCE: Periplaneta americana (L.) (PA) has been used in traditional Chinese medicine for thousands of years for the effect of invigorating blood circulation and removing blood stasis. Modern pharmacological research shown that PA extract exhibits promising effects in promoting wound healing and regeneration, as well as in brain diseases such as Parkinson's disease (PD). However, whether it is effective for neuroregeneration and neurological function recovery after stroke still unknown...
November 10, 2023: Journal of Ethnopharmacology
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