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https://www.readbyqxmd.com/read/29669898/multi-omics-analysis-of-thermal-stress-response-in-a-zooxanthellate-cnidarian-reveals-the-importance-of-associating-with-thermotolerant-symbionts
#1
Maha J Cziesielski, Yi Jin Liew, Guoxin Cui, Sebastian Schmidt-Roach, Sara Campana, Claudius Marondedze, Manuel Aranda
Corals and their endosymbiotic dinoflagellates of the genus Symbiodinium have a fragile relationship that breaks down under heat stress, an event known as bleaching. However, many coral species have adapted to high temperature environments such as the Red Sea (RS). To investigate mechanisms underlying temperature adaptation in zooxanthellate cnidarians we compared transcriptome- and proteome-wide heat stress response (24 h at 32°C) of three strains of the model organism Aiptasia pallida from regions with differing temperature profiles; North Carolina (CC7), Hawaii (H2) and the RS...
April 25, 2018: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29615919/using-aiptasia-as-a-model-to-study-metabolic-interactions-in-cnidarian-symbiodinium-symbioses
#2
Nils Rädecker, Jean-Baptiste Raina, Mathieu Pernice, Gabriela Perna, Paul Guagliardo, Matt R Kilburn, Manuel Aranda, Christian R Voolstra
The symbiosis between cnidarian hosts and microalgae of the genus Symbiodinium provides the foundation of coral reefs in oligotrophic waters. Understanding the nutrient-exchange between these partners is key to identifying the fundamental mechanisms behind this symbiosis, yet has proven difficult given the endosymbiotic nature of this relationship. In this study, we investigated the respective contribution of host and symbiont to carbon and nitrogen assimilation in the coral model anemone Aiptaisa. For this, we combined traditional measurements with nanoscale secondary ion mass spectrometry (NanoSIMS) and stable isotope labeling to investigate patterns of nutrient uptake and translocation both at the organismal scale and at the cellular scale...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29553817/sphingolipid-metabolism-of-a-sea-anemone-is-altered-by-the-presence-of-dinoflagellate-symbionts
#3
Sheila A Kitchen, Angela Z Poole, Virginia M Weis
In host-microbe interactions, signaling lipids function in interpartner communication during both the establishment and maintenance of associations. Previous evidence suggests that sphingolipids play a role in the mutualistic cnidarian-Symbiodinium symbiosis. Exogenously applied sphingolipids have been shown to alter this partnership, though endogenous host regulation of sphingolipids by the sphingosine rheostat under different symbiotic conditions has not been characterized. The rheostat regulates levels of pro-survival sphingosine-1-phosphate (S1P) and pro-apoptotic sphingosine (Sph) through catalytic activities of sphingosine kinase (SPHK) and S1P phosphatase (SGPP)...
December 2017: Biological Bulletin
https://www.readbyqxmd.com/read/29507840/first-insight-into-the-viral-community-of-the-cnidarian-model-metaorganism-aiptasia-using-rna-seq-data
#4
Jan D Brüwer, Christian R Voolstra
Current research posits that all multicellular organisms live in symbioses with associated microorganisms and form so-called metaorganisms or holobionts. Cnidarian metaorganisms are of specific interest given that stony corals provide the foundation of the globally threatened coral reef ecosystems. To gain first insight into viruses associated with the coral model system Aiptasia ( sensu Exaiptasia pallida ), we analyzed an existing RNA-Seq dataset of aposymbiotic, partially populated, and fully symbiotic Aiptasia CC7 anemones with Symbiodinium ...
2018: PeerJ
https://www.readbyqxmd.com/read/29373064/natural-variation-in-responses-to-acute-heat-and-cold-stress-in-a-sea-anemone-model-system-for-coral-bleaching
#5
Emily S Bellis, Dee R Denver
Rising ocean temperatures disrupt the symbiosis between corals and their microalgae, accelerating global decline of coral reef ecosystems. Because of the difficulty of performing laboratory experiments with corals, the sea anemone Aiptasia has emerged as an important model system for molecular studies of coral bleaching and symbiosis. Here, we investigate natural variation in bleaching responses among different genetic lineages of Aiptasia. Both heat- and cold-induced paths to symbiosis breakdown were analyzed...
October 2017: Biological Bulletin
https://www.readbyqxmd.com/read/29218749/evidence-for-mirna-mediated-modulation-of-the-host-transcriptome-in-cnidarian-dinoflagellate-symbiosis
#6
Sebastian Baumgarten, Maha J Cziesielski, Ludivine Thomas, Craig T Michell, Lisl Y Esherick, John R Pringle, Manuel Aranda, Christian R Voolstra
Reef-building corals and other cnidarians living in symbiotic relationships with intracellular, photosynthetic dinoflagellates in the genus Symbiodinium undergo transcriptomic changes during infection with the algae and maintenance of the endosymbiont population. However, the precise regulatory mechanisms modulating the host transcriptome are unknown. Here, we report apparent post-transcriptional gene regulation by miRNAs in the sea anemone Aiptasia, a model system for cnidarian-dinoflagellate endosymbiosis...
December 8, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/29175860/high-salinity-conveys-thermotolerance-in-the-coral-model-aiptasia
#7
Hagen M Gegner, Maren Ziegler, Nils Rädecker, Carol Buitrago-López, Manuel Aranda, Christian R Voolstra
The endosymbiosis between dinoflagellate algae of the genus Symbiodinium and stony corals provides the foundation of coral reef ecosystems. Coral bleaching, the expulsion of endosymbionts from the coral host tissue as a consequence of heat or light stress, poses a threat to reef ecosystem functioning on a global scale. Hence, a better understanding of the factors contributing to heat stress susceptibility and tolerance is needed. In this regard, some of the most thermotolerant corals live in particularly saline habitats, but possible effects of high salinity on thermotolerance in corals are anecdotal...
December 15, 2017: Biology Open
https://www.readbyqxmd.com/read/29167511/transcription-factor-nf-%C3%AE%C2%BAb-is-modulated-by-symbiotic-status-in-a-sea-anemone-model-of-cnidarian-bleaching
#8
Katelyn M Mansfield, Nicole M Carter, Linda Nguyen, Phillip A Cleves, Anar Alshanbayeva, Leah M Williams, Camerron Crowder, Ashley R Penvose, John R Finnerty, Virginia M Weis, Trevor W Siggers, Thomas D Gilmore
Transcription factor NF-κB plays a central role in immunity from fruit flies to humans, and NF-κB activity is altered in many human diseases. To investigate a role for NF-κB in immunity and disease on a broader evolutionary scale we have characterized NF-κB in a sea anemone (Exaiptasia pallida; called Aiptasia herein) model for cnidarian symbiosis and dysbiosis (i.e., "bleaching"). We show that the DNA-binding site specificity of Aiptasia NF-κB is similar to NF-κB proteins from a broad expanse of organisms...
November 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29158383/optimal-nutrient-exchange-and-immune-responses-operate-in-partner-specificity-in-the-cnidarian-dinoflagellate-symbiosis
#9
Jennifer L Matthews, Camerron M Crowder, Clinton A Oakley, Adrian Lutz, Ute Roessner, Eli Meyer, Arthur R Grossman, Virginia M Weis, Simon K Davy
The relationship between corals and dinoflagellates of the genus Symbiodinium is fundamental to the functioning of coral ecosystems. It has been suggested that reef corals may adapt to climate change by changing their dominant symbiont type to a more thermally tolerant one, although the capacity for such a shift is potentially hindered by the compatibility of different host-symbiont pairings. Here we combined transcriptomic and metabolomic analyses to characterize the molecular, cellular, and physiological processes that underlie this compatibility, with a particular focus on Symbiodinium trenchii , an opportunistic, thermally tolerant symbiont that flourishes in coral tissues after bleaching events...
December 12, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28925024/complete-characterization-of-new-isolates-of-neptunomonas-phycophila-leads-to-emend-its-description-and-opens-possibilities-of-biotechnological-applications
#10
Ana L Diéguez, Phillip Pichon, Sabela Balboa, Thorolf Magnesen, Jesús L Romalde
Five strains were isolated from gonad of Great scallop (Pecten maximus) broodstock in a Norwegian hatchery. The study of 16S rRNA gene sequences showed that these isolates belong to Neptunomonas phycophila, a bacterium originally isolated from a symbiont of the anemone Aiptasia tagetes from Puerto Rico. The gyrB and rpoB genes sequences confirmed the affiliation of the scallop isolates to this species. Phenotypic characterization was performed and some differences between the Norwegian isolates and the type strain of N...
December 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28851289/coral-cell-separation-and-isolation-by-fluorescence-activated-cell-sorting-facs
#11
Benyamin Rosental, Zhanna Kozhekbaeva, Nathaniel Fernhoff, Jonathan M Tsai, Nikki Traylor-Knowles
BACKGROUND: Generalized methods for understanding the cell biology of non-model species are quite rare, yet very much needed. In order to address this issue, we have modified a technique traditionally used in the biomedical field for ecological and evolutionary research. Fluorescent activated cell sorting (FACS) is often used for sorting and identifying cell populations. In this study, we developed a method to identify and isolate different cell populations in corals and other cnidarians...
August 29, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28544363/expression-patterns-of-sterol-transporters-npc1-and-npc2-in-the-cnidarian-dinoflagellate-symbiosis
#12
Vincent Dani, Fabrice Priouzeau, Marjolijn Mertz, Magali Mondin, Sophie Pagnotta, Sandra Lacas-Gervais, Simon K Davy, Cécile Sabourault
The symbiotic interaction between cnidarians (e.g., corals and sea anemones) and photosynthetic dinoflagellates of the genus Symbiodinium is triggered by both host-symbiont recognition processes and metabolic exchange between the 2 partners. The molecular communication is crucial for homeostatic regulation of the symbiosis, both under normal conditions and during stresses that further lead to symbiosis collapse. It is therefore important to identify and fully characterise the key players of this intimate interaction at the symbiotic interface...
May 22, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28481198/a-diverse-host-thrombospondin-type-1-repeat-protein-repertoire-promotes-symbiont-colonization-during-establishment-of-cnidarian-dinoflagellate-symbiosis
#13
Emilie-Fleur Neubauer, Angela Z Poole, Philipp Neubauer, Olivier Detournay, Kenneth Tan, Simon K Davy, Virginia M Weis
The mutualistic endosymbiosis between cnidarians and dinoflagellates is mediated by complex inter-partner signaling events, where the host cnidarian innate immune system plays a crucial role in recognition and regulation of symbionts. To date, little is known about the diversity of thrombospondin-type-1 repeat (TSR) domain proteins in basal metazoans or their potential role in regulation of cnidarian-dinoflagellate mutualisms. We reveal a large and diverse repertoire of TSR proteins in seven anthozoan species, and show that in the model sea anemone Aiptasia pallida the TSR domain promotes colonization of the host by the symbiotic dinoflagellate Symbiodinium minutum...
May 8, 2017: ELife
https://www.readbyqxmd.com/read/28474894/thermal-shock-induces-host-proteostasis-disruption-and-endoplasmic-reticulum-stress-in-the-model-symbiotic-cnidarian-aiptasia
#14
Clinton A Oakley, Elysanne Durand, Shaun P Wilkinson, Lifeng Peng, Virginia M Weis, Arthur R Grossman, Simon K Davy
Coral bleaching has devastating effects on coral survival and reef ecosystem function, but many of the fundamental cellular effects of thermal stress on cnidarian physiology are unclear. We used label-free liquid chromatography-tandem mass spectrometry to compare the effects of rapidly (33.5 °C, 24 h) and gradually (30 and 33.5 °C, 12 days) elevated temperatures on the proteome of the model symbiotic anemone Aiptasia. We identified 2133 proteins in Aiptasia, 136 of which were differentially abundant between treatments...
May 11, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28323278/acceptable-symbiont-cell-size-differs-among-cnidarian-species-and-may-limit-symbiont-diversity
#15
Elise Biquand, Nami Okubo, Yusuke Aihara, Vivien Rolland, David C Hayward, Masayuki Hatta, Jun Minagawa, Tadashi Maruyama, Shunichi Takahashi
Reef-building corals form symbiotic relationships with dinoflagellates of the genus Symbiodinium. Symbiodinium are genetically and physiologically diverse, and corals may be able to adapt to different environments by altering their dominant Symbiodinium phylotype. Notably, each coral species associates only with specific Symbiodinium phylotypes, and consequently the diversity of symbionts available to the host is limited by the species specificity. Currently, it is widely presumed that species specificity is determined by the combination of cell-surface molecules on the host and symbiont...
July 2017: ISME Journal
https://www.readbyqxmd.com/read/28272836/mapping-carbon-fate-during-bleaching-in-a-model-cnidarian-symbiosis-the-application-of-13-c-metabolomics
#16
Katie E Hillyer, Daniel A Dias, Adrian Lutz, Ute Roessner, Simon K Davy
Coral bleaching is a major threat to the persistence of coral reefs. Yet we lack detailed knowledge of the metabolic interactions that determine symbiosis function and bleaching-induced change. We mapped autotrophic carbon fate within the free metabolite pools of both partners of a model cnidarian-dinoflagellate symbiosis (Aiptasia-Symbiodinium) during exposure to thermal stress via the stable isotope tracer ((13) C bicarbonate), coupled to GC-MS. Symbiont photodamage and pronounced bleaching coincided with substantial increases in the turnover of non(13) C-labelled pools in the dinoflagellate (lipid and starch store catabolism)...
June 2017: New Phytologist
https://www.readbyqxmd.com/read/28250108/the-sphingosine-rheostat-is-involved-in-the-cnidarian-heat-stress-response-but-not-necessarily-in-bleaching
#17
Sheila A Kitchen, Virginia M Weis
Sphingolipids play important roles in mitigating cellular heat and oxidative stress by altering membrane fluidity, receptor clustering and gene expression. Accumulation of signaling sphingolipids that comprise the sphingosine rheostat, pro-apoptotic sphingosine (Sph) and pro-survival sphingosine-1-phosphate (S1P) is key to determining cell fate. Reef-building corals and other symbiotic cnidarians living in shallow tropical waters can experience elevated seawater temperature and high UV irradiance, two stressors that are increasing in frequency and severity with climate change...
May 1, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28203041/body-size-and-symbiotic-status-influence-gonad-development-in-aiptasia-pallida-anemones
#18
Judith F Carlisle, Grant K Murphy, Alison M Roark
Pale anemones (Aiptasia pallida) coexist with dinoflagellates (primarily Symbiodinium minutum) in a mutualistic relationship. The purpose of this study was to investigate the role of these symbionts in gonad development of anemone hosts. Symbiotic and aposymbiotic anemones were subjected to light cycles that induced gametogenesis. These anemones were then sampled weekly for nine weeks, and gonad development was analyzed histologically. Anemone size was measured as mean body column diameter, and oocytes or sperm follicles were counted for each anemone...
2017: Symbiosis
https://www.readbyqxmd.com/read/27896028/the-scavenger-receptor-repertoire-in-six-cnidarian-species-and-its-putative-role-in-cnidarian-dinoflagellate-symbiosis
#19
Emilie F Neubauer, Angela Z Poole, Virginia M Weis, Simon K Davy
Many cnidarians engage in a mutualism with endosymbiotic photosynthetic dinoflagellates that forms the basis of the coral reef ecosystem. Interpartner interaction and regulation includes involvement of the host innate immune system. Basal metazoans, including cnidarians have diverse and complex innate immune repertoires that are just beginning to be described. Scavenger receptors (SR) are a diverse superfamily of innate immunity genes that recognize a broad array of microbial ligands and participate in phagocytosis of invading microbes...
2016: PeerJ
https://www.readbyqxmd.com/read/27582179/aiptasia-sp-larvae-as-a-model-to-reveal-mechanisms-of-symbiont-selection-in-cnidarians
#20
Iliona Wolfowicz, Sebastian Baumgarten, Philipp A Voss, Elizabeth A Hambleton, Christian R Voolstra, Masayuki Hatta, Annika Guse
Symbiosis, defined as the persistent association between two distinct species, is an evolutionary and ecologically critical phenomenon facilitating survival of both partners in diverse habitats. The biodiversity of coral reef ecosystems depends on a functional symbiosis with photosynthetic dinoflagellates of the highly diverse genus Symbiodinium, which reside in coral host cells and continuously support their nutrition. The mechanisms underlying symbiont selection to establish a stable endosymbiosis in non-symbiotic juvenile corals are unclear...
2016: Scientific Reports
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