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https://www.readbyqxmd.com/read/27907139/contribution-towards-a-metabolite-profile-of-the-detoxification-of-benzoic-acid-through-glycine-conjugation-an-intervention-study
#1
Cindy Irwin, Mari van Reenen, Shayne Mason, Lodewyk J Mienie, Johan A Westerhuis, Carolus J Reinecke
Benzoic acid is widely used as a preservative in food products and is detoxified in humans through glycine conjugation. Different viewpoints prevail on the physiological significance of the glycine conjugation reaction and concerns have been raised on potential public health consequences following uncontrolled benzoic acid ingestion. We performed a metabolomics study which used commercial benzoic acid containing flavored water as vehicle for designed interventions, and report here on the controlled consumption of the benzoic acid by 21 cases across 6 time points for a total of 126 time points...
2016: PloS One
https://www.readbyqxmd.com/read/27896426/expression-and-characterization-of-a-codon-optimized-alkaline-stable-carbonic-anhydrase-from-aliivibrio-salmonicida-for-co2-sequestration-applications
#2
So-Young Jun, Sung Ho Kim, Bashista Kumar Kanth, Jinwon Lee, Seung Pil Pack
The CO2 mineralization process, accelerated by carbonic anhydrase (CA) was proposed for the efficient capture and storage of CO2, the accumulation of which in the atmosphere is the main cause of global warming. Here, we characterize a highly stable form of the cloned CA from the Gram-negative marine bacterium Aliivibrio salmonicida, named ASCA that can promote CO2 absorption in an alkaline solvent required for efficient carbon capture. We designed a mature form of ASCA (mASCA) using a codon optimization of ASCA gene and removal of ASCA signal peptide...
November 28, 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/27887202/serological-markers-in-diagnosis-of-pediatric-inflammatory-bowel-disease-and-as-predictors-for-early-tumor-necrosis-factor-blocker-therapy
#3
Christine Olbjørn, Milada Cvancarova Småstuen, Espen Thiis-Evensen, Britt Nakstad, Morten Harald Vatn, Gøri Perminow
OBJECTIVE: To describe the prevalence of serological markers in newly diagnosed treatment-naïve pediatric inflammatory bowel disease (IBD), their utility in differentiating Crohn's disease (CD), ulcerative colitis (UC) and symptomatic non-IBD patients and whether serological markers are associated with early TNF blocker treatment. MATERIAL AND METHODS: Ninety-six children and adolescents <18 years, 58 with IBD and 38 symptomatic non-IBD controls were included...
November 25, 2016: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/27831916/retrospective-evaluation-of-the-clinical-utility-of-serological-biomarkers-in-chinese-patients-with-inflammatory-bowel-disease-2-year-clinical-experience
#4
Shulan Zhang, Jing Luo, Ji Li, Ziyan Wu, Chaojun Hu, Ping Li, Chuiwen Deng, Fengchun Zhang, Jiaming Qian, Yongzhe Li
BACKGROUND: Antibodies to saccharomyces cerevisiae (ASCA), antibodies to perinuclear anti-neutrophil cytoplasmic (pANCA), pancreatic autoantibodies (PAB) and antibodies against intestinal goblet cells (GAB) are important in diagnosing Crohn's disease (CD) and ulcerative colitis (UC). However, little is known about their diagnostic value in real clinical practice in China. This retrospective study aimed to present our 2-year clinical experience with those biomarkers in diagnosis of CD and UC...
November 10, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/27753692/serologic-anti-gp2-antibodies-are-associated-with-genetic-polymorphisms-fibrostenosis-and-need-for-surgical-resection-in-crohn-s-disease
#5
Frauke Degenhardt, Andrea Dirmeier, Rocio Lopez, Sylvia Lang, Claudia Kunst, Dirk Roggenbuck, Dirk Reinhold, Silke Szymczak, Gerhard Rogler, Frank Klebl, Andre Franke, Florian Rieder
BACKGROUND: The presentation of Crohn's disease (CD) is heterogeneous and often leads to serious complications and need for surgery. We tested serum anti-zymogen granule glycoprotein 2 (GP2) antibodies, including its novel isoform alpha, for association with genetic variants, diagnosis, disease stratification, and prediction of CD courses in a combined cross-sectional and cohort study. METHODS: Serum samples of 303 CD, 108 ulcerative colitis, 72 other inflammatory gastrointestinal diseases, and 206 controls without predominant gastrointestinal diseases controls (HC) were tested for the presence of Anti-GP2 and Anti-Saccharomyces cervisiae (ASCA) by enzyme-linked immunosorbent assay...
November 2016: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/27651359/bacteriome-and-mycobiome-interactions-underscore-microbial-dysbiosis-in-familial-crohn-s-disease
#6
G Hoarau, P K Mukherjee, C Gower-Rousseau, C Hager, J Chandra, M A Retuerto, C Neut, S Vermeire, J Clemente, J F Colombel, H Fujioka, D Poulain, B Sendid, M A Ghannoum
UNLABELLED: Crohn's disease (CD) results from a complex interplay between host genetic factors and endogenous microbial communities. In the current study, we used Ion Torrent sequencing to characterize the gut bacterial microbiota (bacteriome) and fungal community (mycobiome) in patients with CD and their nondiseased first-degree relatives (NCDR) in 9 familial clusters living in northern France-Belgium and in healthy individuals from 4 families living in the same area (non-CD unrelated [NCDU])...
September 20, 2016: MBio
https://www.readbyqxmd.com/read/27649663/the-involvement-of-igh-enhancer-hs1-2-in-the-pathogenesis-of-crohn-s-disease-how-the-immune-system-can-influence-a-multifactorial-disease
#7
R Cianci, S Lolli, D Pagliari, G Gambassi, S Frosali, R Marmo, G Melioli, A Orlando, E E Newton, E Serone, R Landolfi, F Pandolfi, D Frezza
OBJECTIVE: To study the 3' immunoglobulin heavy-chain regulatory region (3'RR) enhancer complex, active in class switching recombination and in B-cells, in Crohn's disease. PATIENTS AND METHODS: A total of 167 patients [79 females (47.3%) and 88 males (52.7%)] affected by Crohn's disease were enrolled in the study. As a control, we included 64 healthy subjects, age and sex matched, from the same geographical area. Blood tests were performed on all subjects to determine their antibody levels and to detect the presence of any possible infections...
September 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27552181/untargeted-identification-of-wood-type-specific-markers-in-particulate-matter-from-wood-combustion
#8
Benedikt A Weggler, Saray Ly-Verdu, Maximilian Jennerwein, Olli Sippula, Ahmed A Reda, Jürgen Orasche, Thomas Gröger, Jorma Jokiniemi, Ralf Zimmermann
Residential wood combustion emissions are one of the major global sources of particulate and gaseous organic pollutants. However, the detailed chemical compositions of these emissions are poorly characterized due to their highly complex molecular compositions, nonideal combustion conditions, and sample preparation steps. In this study, the particulate organic emissions from a masonry heater using three types of wood logs, namely, beech, birch, and spruce, were chemically characterized using thermal desorption in situ derivatization coupled to a GCxGC-ToF/MS system...
September 20, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27455092/anti-food-and-anti-microbial-igg-subclass-antibodies-in-inflammatory-bowel-disease
#9
Anke Jansen, Ana D Mandić, Eveline Bennek, Lisa Frehn, Julien Verdier, Irene Tebrügge, Holger Lutz, Konrad Streetz, Christian Trautwein, Gernot Sellge
OBJECTIVES: Inflammatory bowel disease (IBD), particularly Crohn's disease (CD), is associated with increased microbial-specific IgG and IgA antibodies, whereas alterations of anti-food antibodies are still disputed. The knowledge about IgG subclass antibodies in IBD is limited. In this study we analysed IgG subclass antibodies specific for nutritional and commensal antigens in IBD patients and controls. METHODS: Serum IgG1, IgG2, IgG3 and IgG4 specific for wheat and milk extracts, purified ovalbumin, Escherichia coli and Bacteroides fragilis lysates and mannan from Saccharomyces cerevisiae were analysed by ELISA in patients with CD (n = 56), ulcerative colitis (UC; n = 29), acute gastroenteritis/colitis (n = 12) as well as non-inflammatory controls (n = 62)...
December 2016: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/27282252/physical-chemical-and-kinetic-factors-affecting-prion-infectivity
#10
Francesca Properzi, Anjna Badhan, Steffi Klier, Christian Schmidt, Peter C Klöhn, Jonathan D F Wadsworth, Anthony R Clarke, Graham S Jackson, John Collinge
The mouse-adapted scrapie prion strain RML is one of the most widely used in prion research. The introduction of a cell culture-based assay of RML prions, the scrapie cell assay (SCA) allows more rapid and precise prion titration. A semi-automated version of this assay (ASCA) was applied to explore a range of conditions that might influence the infectivity and properties of RML prions. These include resistance to freeze-thaw procedures; stability to endogenous proteases in brain homogenate despite prolonged exposure to varying temperatures; distribution of infective material between pellet and supernatant after centrifugation, the effect of reducing agents and the influence of detergent additives on the efficiency of infection...
May 3, 2016: Prion
https://www.readbyqxmd.com/read/27148330/metabolic-profile-and-root-development-of-hypericum-perforatum-l-in-vitro-roots-under-stress-conditions-due-to-chitosan-treatment-and-culture-time
#11
Elisa Brasili, Alfredo Miccheli, Federico Marini, Giulia Praticò, Fabio Sciubba, Maria E Di Cocco, Valdir Filho Cechinel, Noemi Tocci, Alessio Valletta, Gabriella Pasqua
The responses of Hypericum perforatum root cultures to chitosan elicitation had been investigated through (1)H-NMR-based metabolomics associated with morpho-anatomical analyses. The root metabolome was influenced by two factors, i.e., time of culture (associated with biomass growth and related "overcrowding stress") and chitosan elicitation. ANOVA simultaneous component analysis (ASCA) modeling showed that these factors act independently. In response to the increase of biomass density over time, a decrease in the synthesis of isoleucine, valine, pyruvate, methylamine, etanolamine, trigonelline, glutamine and fatty acids, and an increase in the synthesis of phenolic compounds, such as xanthones, epicatechin, gallic, and shikimic acid were observed...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27146725/new-nordic-diet-versus-average-danish-diet-a-randomized-controlled-trial-revealed-healthy-long-term-effects-of-the-new-nordic-diet-by-gc-ms-blood-plasma-metabolomics
#12
Bekzod Khakimov, Sanne Kellebjerg Poulsen, Francesco Savorani, Evrim Acar, Gözde Gürdeniz, Thomas M Larsen, Arne Astrup, Lars O Dragsted, Søren Balling Engelsen
A previous study has shown effects of the New Nordic Diet (NND) to stimulate weight loss and lower systolic and diastolic blood pressure in obese Danish women and men in a randomized, controlled dietary intervention study. This work demonstrates long-term metabolic effects of the NND as compared with an Average Danish Diet (ADD) in blood plasma and reveals associations between metabolic changes and health beneficial effects of the NND including weight loss. A total of 145 individuals completed the intervention and blood samples were taken along with clinical examinations before the intervention started (week 0) and after 12 and 26 weeks...
June 3, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27135480/panca-and-asca-in-children-with-ibd-unclassified-crohn-s-colitis-and-ulcerative-colitis-a-longitudinal-report-from-the-ibd-porto-group-of-espghan
#13
Liron Birimberg-Schwartz, David C Wilson, Kaija-Leena Kolho, Katarzyna Karolewska-Bochenek, Nadeem Ahmad Afzal, Christine Spray, Claudio Romano, Paolo Lionetti, Almuthe C Hauer, Christine Martinez-Vinson, Gabor Veres, Johanna C Escher, Dan Turner
INTRODUCTION: No study to date has evaluated perinuclear antineutrophil cytoplasmic antibody (pANCA) and anti-Saccharomyces cerevisiae antibody (ASCA) in pediatric inflammatory bowel disease-unclassified (IBDU) as compared with Crohn's colitis (CC) and ulcerative colitis (UC), which represent the diagnostic challenge. We aimed to explore the diagnostic utility of serology and to assess whether serology can predict disease severity in these subgroups. METHODS: This was a multicenter retrospective longitudinal study including 406 children with inflammatory bowel diseases (IBD) from 23 centers affiliated with the Porto group of European Society of Pediatric Gastroenterology, Hepatology and Nutrition (mean age 10...
August 2016: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/27117843/serologic-microbial-associated-markers-can-predict-crohn-s-disease-behaviour-years-before-disease-diagnosis
#14
R S Choung, F Princen, T P Stockfisch, J Torres, A C Maue, C K Porter, F Leon, B De Vroey, S Singh, M S Riddle, J A Murray, J F Colombel
BACKGROUND: Patients with Crohn's disease (CD) have serologic responses to various microbial antigens. Serologic markers are associated with aggressive forms of disease and can be detected before onset of symptoms. Their utility in pre-clinical disease or prediction of complicated disease course before diagnosis is unclear. AIM: To evaluate the pattern of serologic anti-microbial antibodies long prior to diagnosis and the subsequent risk of complicated Crohn's disease at diagnosis...
June 2016: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/27117106/markers-of-inflammation-activation-of-blood-platelets-and-coagulation-disorders-in-inflammatory-bowel-diseases
#15
REVIEW
Joanna Matowicka-Karna
Inflammatory bowel disease (IBD) includes ulcerative colitis and Crohn's disease. It is a group of chronic disorders characterized by inflammation of the gastrointestinal track with unknown etiology. Currently applied biomarkers include CRP, ESR, pANCA, ASCA, and fecal calprotectin. The etiopathogenesis of IBD is multifactorial. In patients with IBD in inflamed alimentary tract mucosa the number of recruited monocytes and activated macrophages which are source of cytokines. In IBD, the exacerbation is accompanied by thrombocytosis...
2016: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/27111346/assessment-of-fecal-asca-measurement-as-a-biomarker-of-crohn-disease-in-pediatric-patients
#16
Vivian Tang, Clarissa Valim, Rajat Moman, Ashley Richman, Jing Zhou, Veena Ramgopal, Rachel Albert, James H Boone, Paul A Rufo
OBJECTIVES: A simple and reliable biomarker for Crohn disease (CD) would be a valuable clinical tool. We hypothesized that anti-Saccharomyces cerevisiae antibody (ASCA) might be present in the stool of patients with CD. Accordingly, we measured ASCA in the stool and serum of children and adolescents with known or suspected inflammatory bowel disease (IBD). METHODS: We included 114 patients aged ≤ 19 years (73 male) with IBD, including 83 patients with Crohn disease (CD) and 31 subjects without CD (28 with UC, and 3 patients with suspected IBD but without evidence of chronic inflammation at the time of their endoscopy and colonoscopy)...
April 21, 2016: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/27078751/perfluoroalkylated-substance-effects-in-xenopus-laevis-a6-kidney-epithelial-cells-determined-by-atr-ftir-spectroscopy-and-chemometric-analysis
#17
Eva Gorrochategui, Sílvia Lacorte, Romà Tauler, Francis L Martin
The effects of four perfluoroalkylated substances (PFASs), namely, perfluorobutanesulfonate (PFBS), perfluorooctanoic acid (PFOA), perfluorooctanesulfonate (PFOS), and perfluorononanoic acid (PFNA) were assessed in Xenopus laevis A6 kidney epithelial cells by attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy and chemometric analysis. Principal component analysis-linear discriminant analysis (PCA-LDA) was used to visualize wavenumber-related alterations and ANOVA-simultaneous component analysis (ASCA) allowed data processing considering the underlying experimental design...
May 16, 2016: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/27061484/anti-saccharomyces-cerevisiae-antibodies-in-patients-with-anti-%C3%AE-2-glycoprotein-i-antibodies
#18
Amani Mankaï, Skander Layouni, Ibtissem Ghedira
BACKGROUND: In this study, cross-reactive epitopes on β2 glycoprotein I and Saccharomyces cerevisiae have been described. The objective of our study was to determine the frequency of anti S. cerevisiae antibodies (ASCA) in patients with anti-β2 glycoprotein I antibodies (aβ2GPI). METHODS: A retrospective study was conducted in 77 patients with aβ2GPI (aβ2GPI-IgG or aβ2GPI-IgA). Eighty blood donors were used as a control group. ASCA IgG and ASCA IgA were determined by Enzyme Linked Immunosorbent Assay (ELISA)...
April 7, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/26992953/anti-saccharomyces-cerevisiae-antibodies-asca-in-spondyloarthritis-prevalence-and-associated-phenotype
#19
Jérémy Maillet, Sébastien Ottaviani, Florence Tubach, Carine Roy, Pascale Nicaise-Rolland, Elisabeth Palazzo, Philippe Dieudé
OBJECTIVE: The aim of the study was to compare the prevalence of ASCA in spondyloarthrites (SpA) patients and to investigate the association between ASCA status and disease phenotype. METHODS: We performed a case-control study including SpA individuals fulfilling the ESSG SpA criteria. The following data were collected for analysis: gender, age, disease duration, clinical or associated features of SpA, treatments, HLAB27 and ASCA status. A control group of patients without SpA was also analyzed...
March 15, 2016: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/26982193/asca-anca-alca-and-many-more-are-they-useful-in-the-diagnosis-of-inflammatory-bowel-disease
#20
Guangxi Zhou, Yang Song, Wenjing Yang, Yanmin Guo, Leilei Fang, Yamei Chen, Zhanju Liu
BACKGROUND: Inflammatory bowel disease (IBD) is characterized by excessive immune responses to altered intestinal microbiota in genetically susceptible individuals. The diagnosis of IBD depends on clinical, endoscopic, histological, radiological and biochemical criteria, which may be invasive, time consuming and usually not accepted by patients with IBD. KEY MESSAGES: Serological biomarkers have been demonstrated to be a series of rapid, non-invasive approaches for assessments of early diagnosis, disease activity and prognosis for IBD...
2016: Digestive Diseases
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