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https://www.readbyqxmd.com/read/29034577/untargeted-gc-ms-metabolomics-reveals-changes-in-the-metabolite-dynamics-of-industrial-scale-batch-fermentations-of-streptoccoccus-thermophilus-broth
#1
Bekzod Khakimov, Lene D Christiansen, Anna-Lena Heins, Klavs M Sørensen, Charlotte Schöller, Anders Clausen, Thomas Skov, Krist V Gernaey, Søren B Engelsen
An industrial scale biomass production using batch or fed-batch fermentations usually optimized by selection of bacterial strains, tuning fermentation media, feeding strategy, and temperature. However, in-depth investigation of the biomass metabolome during the production may reveal new knowledge for better optimization. In this study, for the first time, the authors investigated seven fermentation batches performed on five Streptoccoccus thermophilus strains during the biomass production at Chr. Hansen (Denmark) in a real life large scale fermentation process...
October 16, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28822928/combined-effects-of-salinity-temperature-and-hypoxia-on-daphnia-magna-metabolism
#2
Elba Garreta-Lara, Bruno Campos, Carlos Barata, Silvia Lacorte, Romà Tauler
Metabolic changes of Daphnia magna pools due different abiotic factors linked to global climate change (salinity, temperature and hypoxia) were investigated using untargeted GC-MS and advanced chemometric strategies using a three factors two-level full factorial experimental design (DoE). Effects of these three factors and identity of the metabolites whose concentrations changed because of them were investigated. The simultaneous analysis of GC-MS data sets using Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) allowed the resolution of the elution and mass spectra profiles of a large number of D...
January 1, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/28802139/analysis-of-neurobehavioural-data-by-chemometric-methods-in-ecotoxicological-studies
#3
Cristian Gómez-Canela, Eva Prats, Romà Tauler, Demetrio Raldúa
Incorporation of chemometric tools in behavioural data management workflows allows for the early identification of most relevant endpoints complementarily to statistical confirmatory approaches. In this work, the effects of two model neurotoxicants, chlorpyrifos (CPF) and nicotine, exposures on behavioural profiles of adult zebrafish at three different times (2, 6 and 24h) were evaluated using open field test (OFT) paradigm experiments. Two chemometric methods like Principal Component Analysis (PCA) and Analysis of Variance-Simultaneous Component Analysis (ASCA) have been used to interpret the changes observed in the obtained behavioural data...
August 9, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28745018/a-comparison-between-linguistic-skills-and-socio-communicative-abilities-in-williams-syndrome
#4
P Alfieri, D Menghini, L Marotta, L De Peppo, L Ravà, F Salvaguardia, C Varuzza, S Vicari
BACKGROUND: Individuals with Williams syndrome (WS) show a disharmonic linguistic profile with a clear pattern of strengths and weaknesses. Despite their sociable nature, atypical socio-communicative abilities and deficits in communication and relationship with others have been found. AIM: The aim of the present study was to investigate whether linguistic skills (LS) were in line with the pragmatic and social use of language and the cognitive development of 32 individuals with WS (18 boys and 14 girls) with a mean chronological age of 12...
September 2017: Journal of Intellectual Disability Research: JIDR
https://www.readbyqxmd.com/read/28725447/anti-saccharomyces-cerevisiae-antibodies-asca-are-associated-with-body-fat-mass-and-systemic-inflammation-but-not-with-dietary-yeast-consumption-a-cross-sectional-study
#5
Anne Stine Kvehaugen, Martin Aasbrenn, Per G Farup
BACKGROUND: Baker's/brewer's yeast, Saccharomyces cerevisiae, has been used as an alternative to antibiotic growth promoters to improve growth performance in animals. In humans, Saccharomyces cerevisiae is among the most commonly detected fungi in fecal samples and likely originates from food. Recently, an association between anti-Saccharomyces cerevisiae antibodies (ASCA) and obesity in humans was suggested, but the cause of the elevated ASCA levels is not clear. Our aim was to study ASCA in morbidly obese subjects and explore potential associations with anthropometrics, diet, co-morbidities and biomarkers of inflammation and gut permeability...
2017: BMC Obesity
https://www.readbyqxmd.com/read/28692288/toward-reliable-lipoprotein-particle-predictions-from-nmr-spectra-of-human-blood-an-interlaboratory-ring-test
#6
Sandra Monsonis Centelles, Huub C J Hoefsloot, Bekzod Khakimov, Parvaneh Ebrahimi, Mads V Lind, Mette Kristensen, Niels de Roo, Doris M Jacobs, John van Duynhoven, Claire Cannet, Fang Fang, Eberhard Humpfer, Hartmut Schäfer, Manfred Spraul, Søren B Engelsen, Age K Smilde
Lipoprotein profiling of human blood by (1)H nuclear magnetic resonance (NMR) spectroscopy is a rapid and promising approach to monitor health and disease states in medicine and nutrition. However, lack of standardization of measurement protocols has prevented the use of NMR-based lipoprotein profiling in metastudies. In this study, a standardized NMR measurement protocol was applied in a ring test performed across three different laboratories in Europe on plasma and serum samples from 28 individuals. Data was evaluated in terms of (i) spectral differences, (ii) differences in LPD predictions obtained using an existing prediction model, and (iii) agreement of predictions with cholesterol concentrations in high- and low-density lipoproteins (HDL and LDL) particles measured by standardized clinical assays...
August 1, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28664835/prevalence-and-significance-of-anti-saccharomyces-cerevisiae-antibodies-in-primary-sj%C3%A3-gren-s-syndrome
#7
Alessia Alunno, Onelia Bistoni, Francesco Carubbi, Valentina Valentini, Giacomo Cafaro, Elena Bartoloni Bocci, Roberto Giacomelli, Roberto Gerli
OBJECTIVES: Saccharomyces cerevisiae is a common yeast used in the food industry. IgG and IgA antibodies against the phosphopeptidomannan of the S. cerevisiae cell wall (ASCA) are a well known marker of disease severity in Crohn's disease. Moreover, a number of studies assessed ASCA in several systemic and organ-specific autoimmune diseases postulating molecular mimicry as a possible link between ASCA and autoimmunity. However, since they have never been tested in primary Sjögren's syndrome (pSS), the purpose of this study was to investigate these antibodies in a large cohort of pSS patients, compared to healthy donors (HD), and their significance as potentially helpful biomarker in a clinical setting...
June 29, 2017: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/28661185/the-value-of-serum-antibodies-in-differentiating-inflammatory-bowel-disease-predicting-disease-activity-and-disease-course-in-the-newly-diagnosed-patient
#8
Carolijn Smids, Carmen S Horjus Talabur Horje, Marcel J M Groenen, Elly H M van Koolwijk, Peter J Wahab, Ellen G van Lochem
BACKGROUND: Data on serum antibodies in untreated adult inflammatory bowel disease (IBD) patients at diagnosis are scarcely available, and results on the stability of antibody presence over time are inconsistent. Our aim was to investigate antibodies in newly diagnosed, untreated IBD patients in relation to disease phenotype and course. Furthermore, we analyzed antibody presence over time. METHODS: Baseline anti-Saccharomyces cerevisiae antibodies (ASCA), anti-chitobioside carbohydrate antibodies (ACCA), anti-laminaribioside carbohydrate antibodies (ALCA) and anti-mannobioside carbohydrate antibodies (AMCA) were measured with enzyme-linked immunosorbent assays and perinuclear anti-neutrophilic cytoplasmic antibodies (pANCA) was measured by indirect immunofluorescence in serum of 120 untreated IBD patients at diagnosis and 19 healthy controls...
June 29, 2017: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/28505469/the-association-between-immune-markers-and-recent-suicide-attempts-in-patients-with-serious-mental-illness-a-pilot-study
#9
Faith Dickerson, Maria Adamos, Emily Katsafanas, Sunil Khushalani, Andrea Origoni, Christina Savage, Lucy Schweinfurth, Cassie Stallings, Kevin Sweeney, Armin Alaedini, Melanie Uhde, Emily Severance, Holly C Wilcox, Robert Yolken
Previous studies have identified elevations in markers of gastrointestinal inflammation in schizophrenia and mood disorders but studies have not measured the association between these markers and recent suicide attempts. We assessed 210 patients receiving treatment for schizophrenia, bipolar disorder, or major depression. We employed the Columbia Suicide Severity Rating Scale to identify recent and lifetime suicide attempts (actual, aborted, and interrupted). Psychiatric participants and a control group of 72 individuals without a psychiatric disorder had a blood sample drawn from which were measured specific markers of gastrointestinal inflammation and also C-Reactive protein (CRP)...
September 2017: Psychiatry Research
https://www.readbyqxmd.com/read/28413638/serologic-testing-of-a-panel-of-five-antibodies-in-inflammatory-bowel-diseases-diagnostic-value-and-correlation-with-disease-phenotype
#10
Zhi-Zhi Wang, Ke Shi, Jie Peng
The aim of the present study was to evaluate the diagnostic value of five serological antibodies, perinuclear antineutrophil cytoplasmic antibody (pANCA), anti-Saccharomyces cerevisiae antibodies [ASCA; ASCA-immunoglobulin (IgG)and ASCA-IgA], Escherichia coli outer membrane porin C antibody (anti-OmpC) and CBir1 flagellin antibody for detection in inflammatory bowel diseases. Whether the antibody status correlated with the disease phenotype was also evaluated. Sera from 71 patients with Crohn's disease (CD), 41 patients with ulcerative colitis (UC), 78 patients with other gastrointestinal diseases and 31 healthy control subjects were investigated...
April 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28344794/does-calprotectin-level-identify-a-subgroup-among-patients-suffering-from-irritable-bowel-syndrome-results-of-a-prospective-study
#11
Chloé Melchior, Moutaz Aziz, Typhaine Aubry, Guillaume Gourcerol, Muriel Quillard, Alberto Zalar, Moïse Coëffier, Pierre Dechelotte, Anne-Marie Leroi, Philippe Ducrotté
BACKGROUND: Irritable bowel syndrome is a multifactorial disease. Although faecal calprotectin has been shown to be a reliable marker of intestinal inflammation, its role in irritable bowel syndrome remains debated. OBJECTIVE: The aims of this prospective study were to select a subgroup of irritable bowel syndrome patients and to characterise those patients with high faecal calprotectin by systematic work-up. METHODS: Calprotectin levels were determined by enzyme-linked immunosorbent assay test in consecutive irritable bowel syndrome patients fulfilling Rome III criteria in whom normal colonoscopy and appropriate tests had excluded organic disease...
March 2017: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/28261032/eosinophilic-colitis-in-children
#12
Urszula Grzybowska-Chlebowczyk, Sabina Horowska-Ziaja, Maciej Kajor, Sabina Więcek, Wojciech Chlebowczyk, Halina Woś
INTRODUCTION: Eosinophilic colitis, which is a rare form of eosinophilic gastrointestinal diseases, occurs as primary and secondary allergic eosinophilic colitis of the gastrointestinal tract infection, inflammatory bowel disease, celiac disease, and vasculitis. The diagnosis is based on a significant amount of eosinophils in the inflammatory infiltrate of the colon wall. AIM: To analyze the clinical picture taking into account comorbidities and endoscopic picture in children with eosinophilic colitis...
February 2017: Postȩpy Dermatologii i Alergologii
https://www.readbyqxmd.com/read/28243597/early-effect-of-amyloid-%C3%AE-peptide-on-hippocampal-and-serum-metabolism-in-rats-studied-by-an-integrated-method-of-nmr-based-metabolomics-and-anova-simultaneous-component-analysis
#13
Yao Du, Hong Zheng, Huanhuan Xia, Liangcai Zhao, Wenyi Hu, Guanghui Bai, Zhihan Yan, Hongchang Gao
Amyloid β (Aβ) deposition has been implicated in the pathogenesis of Alzheimer's disease. However, the early effect of Aβ deposition on metabolism remains unclear. In the present study, thus, we explored the metabolic changes in the hippocampus and serum during first 2 weeks of Aβ25-35 injection in rats by using an integrated method of NMR-based metabolomics and ANOVA-simultaneous component analysis (ASCA). Our results show that Aβ25-35 injection, time, and their interaction had statistically significant effects on the hippocampus and serum metabolome...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28125550/o-002-predicting-a-complicated-course-for-children-newly-diagnosed-with-crohn-s-disease-a-multicenter-inception-cohort-study
#14
Subra Kugathasan, Lee Denson, Thomas Walters, Mi-Ok Kim, Urko Marigorta, Melanie Schirmer, Kajari Mondal, Chunyan Liu, Michael Stephens, Robert Baldassano, James Markowitz, Ramnik Xavier, Bruce Aronow, Greg Gibson, Jeffrey Hyams, Marla Dubinsky
BACKGROUND: A subset of patients with Crohn's disease (CD) rapidly progress to a stricturing or internal penetrating phenotype that requires surgery. Despite great strides in therapy, there have not been population-level declines in the rates of these serious complications or need for surgery in CD. Effective risk stratification models to predict progression to such complications in pediatric CD are presently lacking. We aimed to develop and test a prognostic model incorporating clinical and host biology and integrated the comparative effectiveness of anti-TNF in preventing stricturing or perforating complications...
February 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/27976801/serologic-antibodies-in-relation-to-outcome-in-post-operative-crohn-s-disease
#15
Amy L Hamilton, Michael A Kamm, Peter De Cruz, Emily K Wright, Fabiyola Selvaraj, Fred Princen, Alexandra Gorelik, Danny Liew, Ian C Lawrance, Jane M Andrews, Peter A Bampton, Miles P Sparrow, Timothy H Florin, Peter R Gibson, Henry Debinski, Richard B Gearry, Finlay A Macrae, Rupert W Leong, Ian Kronborg, Graham Radford-Smith, Warwick Selby, Sally J Bell, Steven J Brown, William R Connell
BACKGROUND: Disease recurs frequently after Crohn's disease resection. The role of serological antimicrobial antibodies in predicting recurrence or as a marker of recurrence has not been well defined. METHODS: 169 patients (523 samples) were prospectively studied, with testing peri-operatively, and 6, 12 and 18 months post-operatively. Colonoscopy was performed at 18 months post-operatively. Serologic antibody presence (pANCA, ASCA IgA/IgG, anti-OmpC, anti-CBir1, anti-A4-Fla2, anti-Fla-X) and titre were tested...
December 15, 2016: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/27907139/contribution-towards-a-metabolite-profile-of-the-detoxification-of-benzoic-acid-through-glycine-conjugation-an-intervention-study
#16
Cindy Irwin, Mari van Reenen, Shayne Mason, Lodewyk J Mienie, Johan A Westerhuis, Carolus J Reinecke
Benzoic acid is widely used as a preservative in food products and is detoxified in humans through glycine conjugation. Different viewpoints prevail on the physiological significance of the glycine conjugation reaction and concerns have been raised on potential public health consequences following uncontrolled benzoic acid ingestion. We performed a metabolomics study which used commercial benzoic acid containing flavored water as vehicle for designed interventions, and report here on the controlled consumption of the benzoic acid by 21 cases across 6 time points for a total of 126 time points...
2016: PloS One
https://www.readbyqxmd.com/read/27896426/expression-and-characterization-of-a-codon-optimized-alkaline-stable-carbonic-anhydrase-from-aliivibrio-salmonicida-for-co2-sequestration-applications
#17
So-Young Jun, Sung Ho Kim, Bashista Kumar Kanth, Jinwon Lee, Seung Pil Pack
The CO2 mineralization process, accelerated by carbonic anhydrase (CA) was proposed for the efficient capture and storage of CO2, the accumulation of which in the atmosphere is the main cause of global warming. Here, we characterize a highly stable form of the cloned CA from the Gram-negative marine bacterium Aliivibrio salmonicida, named ASCA that can promote CO2 absorption in an alkaline solvent required for efficient carbon capture. We designed a mature form of ASCA (mASCA) using a codon optimization of ASCA gene and removal of ASCA signal peptide...
March 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/27887202/serological-markers-in-diagnosis-of-pediatric-inflammatory-bowel-disease-and-as-predictors-for-early-tumor-necrosis-factor-blocker-therapy
#18
Christine Olbjørn, Milada Cvancarova Småstuen, Espen Thiis-Evensen, Britt Nakstad, Morten Harald Vatn, Gøri Perminow
OBJECTIVE: To describe the prevalence of serological markers in newly diagnosed treatment-naïve pediatric inflammatory bowel disease (IBD), their utility in differentiating Crohn's disease (CD), ulcerative colitis (UC) and symptomatic non-IBD patients and whether serological markers are associated with early TNF blocker treatment. MATERIAL AND METHODS: Ninety-six children and adolescents <18 years, 58 with IBD and 38 symptomatic non-IBD controls were included...
April 2017: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/27831916/retrospective-evaluation-of-the-clinical-utility-of-serological-biomarkers-in-chinese-patients-with-inflammatory-bowel-disease-2-year-clinical-experience
#19
Shulan Zhang, Jing Luo, Ji Li, Ziyan Wu, Chaojun Hu, Ping Li, Chuiwen Deng, Fengchun Zhang, Jiaming Qian, Yongzhe Li
BACKGROUND: Antibodies to saccharomyces cerevisiae (ASCA), antibodies to perinuclear anti-neutrophil cytoplasmic (pANCA), pancreatic autoantibodies (PAB) and antibodies against intestinal goblet cells (GAB) are important in diagnosing Crohn's disease (CD) and ulcerative colitis (UC). However, little is known about their diagnostic value in real clinical practice in China. This retrospective study aimed to present our 2-year clinical experience with those biomarkers in diagnosis of CD and UC...
May 1, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/27753692/serologic-anti-gp2-antibodies-are-associated-with-genetic-polymorphisms-fibrostenosis-and-need-for-surgical-resection-in-crohn-s-disease
#20
Frauke Degenhardt, Andrea Dirmeier, Rocio Lopez, Sylvia Lang, Claudia Kunst, Dirk Roggenbuck, Dirk Reinhold, Silke Szymczak, Gerhard Rogler, Frank Klebl, Andre Franke, Florian Rieder
BACKGROUND: The presentation of Crohn's disease (CD) is heterogeneous and often leads to serious complications and need for surgery. We tested serum anti-zymogen granule glycoprotein 2 (GP2) antibodies, including its novel isoform alpha, for association with genetic variants, diagnosis, disease stratification, and prediction of CD courses in a combined cross-sectional and cohort study. METHODS: Serum samples of 303 CD, 108 ulcerative colitis, 72 other inflammatory gastrointestinal diseases, and 206 controls without predominant gastrointestinal diseases controls (HC) were tested for the presence of Anti-GP2 and Anti-Saccharomyces cervisiae (ASCA) by enzyme-linked immunosorbent assay...
November 2016: Inflammatory Bowel Diseases
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