keyword
https://read.qxmd.com/read/38536819/stem-cell-derived-vessels-on-chip-for-cardiovascular-disease-modeling
#1
JOURNAL ARTICLE
Maren Marder, Caroline Remmert, Julius A Perschel, Munkhtur Otgonbayar, Christine von Toerne, Stefanie Hauck, Judith Bushe, Annette Feuchtinger, Bilal Sheikh, Michel Moussus, Matthias Meier
The metabolic syndrome is accompanied by vascular complications. Human in vitro disease models are hence required to better understand vascular dysfunctions and guide clinical therapies. Here, we engineered an open microfluidic vessel-on-chip platform that integrates human pluripotent stem cell-derived endothelial cells (SC-ECs). The open microfluidic design enables seamless integration with state-of-the-art analytical technologies, including single-cell RNA sequencing, proteomics by mass spectrometry, and high-resolution imaging...
March 26, 2024: Cell Reports
https://read.qxmd.com/read/38535481/cell-reprogramming-and-differentiation-utilizing-messenger-rna-for-regenerative-medicine
#2
REVIEW
Masahito Inagaki
The COVID-19 pandemic generated interest in the medicinal applications of messenger RNA (mRNA). It is expected that mRNA will be applied, not only to vaccines, but also to regenerative medicine. The purity of mRNA is important for its medicinal applications. However, the current mRNA synthesis techniques exhibit problems, including the contamination of undesired 5'-uncapped mRNA and double-stranded RNA. Recently, our group developed a completely capped mRNA synthesis technology that contributes to the progress of mRNA research...
December 20, 2023: Journal of Developmental Biology
https://read.qxmd.com/read/38534877/effect-of-fibrillin-2-on-differentiation-into-periodontal-ligament-stem-cell-like-cells-derived-from-human-induced-pluripotent-stem-cells
#3
JOURNAL ARTICLE
Sayuri Hamano, Daiki Yamashita, Daigaku Hasegawa, Hideki Sugii, Tomohiro Itoyama, Hidefumi Maeda
Periodontal tissue regeneration is important for preserving teeth. Periodontal ligament stem cells (PDLSCs) are useful in periodontal tissue regeneration, however tooth extraction is required to obtain these cells. Therefore, we focused on induced pluripotent stem (iPS) cells and established a method to obtain PDLSC-like cells from iPS cells. Specifically, we first differentiated iPS cells into neural crest-like cells (iNCs). Next, we obtained PDLSC-like cells (iPDLSCs) by culturing iNCs on extracellular matrix (ECM) derived from human primary periodontal ligament cells (HPDLCs)...
March 27, 2024: Stem Cells and Development
https://read.qxmd.com/read/38534757/to-repair-a-broken-heart-stem-cells-in-ischemic-heart-disease
#4
REVIEW
Theodora M Stougiannou, Konstantinos C Christodoulou, Ioannis Dimarakis, Dimitrios Mikroulis, Dimos Karangelis
Despite improvements in contemporary medical and surgical therapies, cardiovascular disease (CVD) remains a significant cause of worldwide morbidity and mortality; more specifically, ischemic heart disease (IHD) may affect individuals as young as 20 years old. Typically managed with guideline-directed medical therapy, interventional or surgical methods, the incurred cardiomyocyte loss is not always completely reversible; however, recent research into various stem cell (SC) populations has highlighted their potential for the treatment and perhaps regeneration of injured cardiac tissue, either directly through cellular replacement or indirectly through local paracrine effects...
March 8, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534508/contractile-and-genetic-characterization-of-cardiac-constructs-engineered-from-human-induced-pluripotent-stem-cells-modeling-of-tuberous-sclerosis-complex-and-the-effects-of-rapamycin
#5
JOURNAL ARTICLE
Veniamin Y Sidorov, Tatiana N Sidorova, Philip C Samson, Ronald S Reiserer, Clayton M Britt, M Diana Neely, Kevin C Ess, John P Wikswo
The implementation of three-dimensional tissue engineering concurrently with stem cell technology holds great promise for in vitro research in pharmacology and toxicology and modeling cardiac diseases, particularly for rare genetic and pediatric diseases for which animal models, immortal cell lines, and biopsy samples are unavailable. It also allows for a rapid assessment of phenotype-genotype relationships and tissue response to pharmacological manipulation. Mutations in the TSC1 and TSC2 genes lead to dysfunctional mTOR signaling and cause tuberous sclerosis complex (TSC), a genetic disorder that affects multiple organ systems, principally the brain, heart, skin, and kidneys...
February 28, 2024: Bioengineering
https://read.qxmd.com/read/38534402/transgene-free-cynomolgus-monkey-ipscs-generated-under-chemically-defined-conditions
#6
JOURNAL ARTICLE
Yuliia Tereshchenko, Nesil Esiyok, Enrique Garea-Rodríguez, Daniele Repetto, Rüdiger Behr, Ignacio Rodríguez-Polo
Non-human primates (NHPs) are pivotal animal models for translating novel cell replacement therapies into clinical applications, including validating the safety and efficacy of induced pluripotent stem cell (iPSC)-derived products. Preclinical development and the testing of cell-based therapies ideally comprise xenogeneic (human stem cells into NHPs) and allogenic (NHP stem cells into NHPs) transplantation studies. For the allogeneic approach, it is necessary to generate NHP-iPSCs with generally equivalent quality to the human counterparts that will be used later on in patients...
March 21, 2024: Cells
https://read.qxmd.com/read/38534378/deep-learning-powered-identification-of-differentiated-early-mesoderm-cells-from-pluripotent-stem-cells
#7
JOURNAL ARTICLE
Sakib Mohammad, Arpan Roy, Andreas Karatzas, Sydney L Sarver, Iraklis Anagnostopoulos, Farhan Chowdhury
Pluripotent stem cells can be differentiated into all three germ-layers including ecto-, endo-, and mesoderm in vitro. However, the early identification and rapid characterization of each germ-layer in response to chemical and physical induction of differentiation is limited. This is a long-standing issue for rapid and high-throughput screening to determine lineage specification efficiency. Here, we present deep learning (DL) methodologies for predicting and classifying early mesoderm cells differentiated from embryoid bodies (EBs) based on cellular and nuclear morphologies...
March 18, 2024: Cells
https://read.qxmd.com/read/38534355/recent-research-trends-in-neuroinflammatory-and-neurodegenerative-disorders
#8
REVIEW
Jessica Cohen, Annette Mathew, Kirk D Dourvetakis, Estella Sanchez-Guerrero, Rajendra P Pangeni, Narasimman Gurusamy, Kristina K Aenlle, Geeta Ravindran, Assma Twahir, Dylan Isler, Sara Rukmini Sosa-Garcia, Axel Llizo, Alison C Bested, Theoharis C Theoharides, Nancy G Klimas, Duraisamy Kempuraj
Neuroinflammatory and neurodegenerative disorders including Alzheimer's disease (AD), Parkinson's disease (PD), traumatic brain injury (TBI) and Amyotrophic lateral sclerosis (ALS) are chronic major health disorders. The exact mechanism of the neuroimmune dysfunctions of these disease pathogeneses is currently not clearly understood. These disorders show dysregulated neuroimmune and inflammatory responses, including activation of neurons, glial cells, and neurovascular unit damage associated with excessive release of proinflammatory cytokines, chemokines, neurotoxic mediators, and infiltration of peripheral immune cells into the brain, as well as entry of inflammatory mediators through damaged neurovascular endothelial cells, blood-brain barrier and tight junction proteins...
March 14, 2024: Cells
https://read.qxmd.com/read/38534351/optical-genome-mapping-reveals-genomic-alterations-upon-gene-editing-in-hipscs-implications-for-neural-tissue-differentiation-and-brain-organoid-research
#9
JOURNAL ARTICLE
Lucia Gallego Villarejo, Wanda M Gerding, Lisa Bachmann, Luzie H I Hardt, Stefan Bormann, Huu Phuc Nguyen, Thorsten Müller
Genome editing, notably CRISPR (cluster regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9), has revolutionized genetic engineering allowing for precise targeted modifications. This technique's combination with human induced pluripotent stem cells (hiPSCs) is a particularly valuable tool in cerebral organoid (CO) research. In this study, CRISPR/Cas9-generated fluorescently labeled hiPSCs exhibited no significant morphological or growth rate differences compared with unedited controls...
March 14, 2024: Cells
https://read.qxmd.com/read/38533916/exosomes-secreted-from-induced-pluripotent-stem-cell-ameliorate-the-lipopolysaccharide-induced-neuroinflammatory-response-via-lncrna-0949
#10
JOURNAL ARTICLE
Lixiu Ma, Ce Xiao, Zhizhe Zhang, Yi-An Zhan
PURPOSE: To study the effect of exosomes derived from the induced pluripotent stem cells (iPSCs) in the neuroinflammatory response of microglia caused by lipopolysaccharide (LPS) and reveal the potential underlying mechanism. METHODS: A permanent microglia cell line HMO6 was activated by LPS. The features of exosomes were analyzed by nano flow cytometry, Western blot and transmission electron microscope. The RNA-seq was used to analyze the difference of noncoding RNA profiles between iPSC-Exos and HMO6 derived exosomes and proved that long no-coding RNA (lncRNA-0949) was highly expressed in the iPSC-Exos...
March 2024: Immunity, Inflammation and Disease
https://read.qxmd.com/read/38533750/an-optimized-protocol-for-routine-development-of-cell-culture-from-adult-oyster-crassostrea-madrasensis
#11
JOURNAL ARTICLE
Soumya Balakrishnan, Ambadi Kannan Maliyekkal Sajeevan, Sreevidya Chandrasekharan Parvathi, I S Bright Singh, Jayesh Puthumana
Marine molluscan cell lines, required for virus screening and cultivation, form essential tools for developing health management strategies for these animals in the blue economy. Moreover, they are also crucial to develop cultivated seafood. As there is no valid marine molluscan cell line, primary cell cultures are relied upon for all investigations. A sound protocol for generating primary cell cultures from molluscs is entailed, but existing protocols often involve heavy antibiotic usage and depuration that invariably affect gene expression and cell health...
March 27, 2024: Cell Biology International
https://read.qxmd.com/read/38533521/embryo-and-fetal-gene-editing-technical-challenges-and-progress-toward-clinical-applications
#12
REVIEW
Citra N Z Mattar, Wei Leong Chew, Poh San Lai
Gene modification therapies (GMTs) are slowly but steadily making progress toward clinical application. As the majority of rare diseases have an identified genetic cause, and as rare diseases collectively affect 5% of the global population, it is increasingly important to devise gene correction strategies to address the root causes of the most devastating of these diseases and to provide access to these novel therapies to the most affected populations. The main barriers to providing greater access to GMTs continue to be the prohibitive cost of developing these novel drugs at clinically relevant doses, subtherapeutic effects, and toxicity related to the specific agents or high doses required...
June 13, 2024: Molecular Therapy. Methods & Clinical Development
https://read.qxmd.com/read/38533065/pcgf5-an-important-regulatory-factor-in-early-embryonic-neural-induction
#13
JOURNAL ARTICLE
Xuan Yang, Wenjuan Zhou, Juan Zhou, Anna Li, Changqing Zhang, Zhenya Fang, Chunying Wang, Shiyu Liu, Aijun Hao, Meihua Zhang
Polycomb group RING finger (PCGF) proteins, a crucial subunits of the Polycomb complex, plays an important role in regulating gene expression, embryonic development, and cell fate determination. In our research, we investigated Pcgf5, one of the six PCGF homologs, and its impact on the differentiation of P19 cells into neural stem cells. Our findings revealed that knockdown of Pcgf5 resulted in a significant decrease in the expression levels of the neuronal markers Sox2, Zfp521, and Pax6, while the expression levels of the pluripotent markers Oct4 and Nanog increased...
March 30, 2024: Heliyon
https://read.qxmd.com/read/38532875/piv-myomonitor-an-accessible-particle-image-velocimetry-based-software-tool-for-advanced-contractility-assessment-of-cardiac-organoids
#14
JOURNAL ARTICLE
Hoyeon Lee, Boyoung Kim, Jiyue Yun, Jinseung Bae, Sungsu Park, Junseok Jeon, Hye Ryoun Jang, Jaecheol Lee, Soah Lee
Induced pluripotent stem cell (iPSC)-derived cardiac organoids offer a versatile platform for personalized cardiac toxicity assessment, drug screening, disease modeling, and regenerative therapies. While previous image-based contractility analysis techniques allowed the assessment of contractility of two-dimensional cardiac models, they face limitations, including encountering high noise levels when applied to three-dimensional organoid models and requiring expensive equipment. Additionally, they offer fewer functional parameters compared to commercial software...
2024: Frontiers in Bioengineering and Biotechnology
https://read.qxmd.com/read/38532378/biosafe-cerium-oxide-nanozymes-protect-human-pluripotent-stem-cells-and-cardiomyocytes-from-oxidative-stress
#15
JOURNAL ARTICLE
Chengwen Hang, Mohamed S Moawad, Zheyi Lin, Huixin Guo, Hui Xiong, Mingshuai Zhang, Renhong Lu, Junyang Liu, Dan Shi, Duanyang Xie, Yi Liu, Dandan Liang, Yi-Han Chen, Jian Yang
BACKGROUND: Cardiovascular diseases (CVDs) have the highest mortality worldwide. Human pluripotent stem cells (hPSCs) and their cardiomyocyte derivatives (hPSC-CMs) offer a valuable resource for disease modeling, pharmacological screening, and regenerative therapy. While most CVDs are linked to significant over-production of reactive oxygen species (ROS), the effects of current antioxidants targeting excessive ROS are limited. Nanotechnology is a powerful tool to develop antioxidants with improved selectivity, solubility, and bioavailability to prevent or treat various diseases related to oxidative stress...
March 26, 2024: Journal of Nanobiotechnology
https://read.qxmd.com/read/38532120/nilotinib-induced-alterations-in-endothelial-cell-function-recapitulate-clinical-vascular-phenotypes-independent-of-abl1
#16
JOURNAL ARTICLE
Emily A Pinheiro, Jean-Marc DeKeyser, Brian Lenny, Yadav Sapkota, Paul W Burridge
Nilotinib is a highly effective treatment for chronic myeloid leukemia but has been consistently associated with the development of nilotinib-induced arterial disease (NAD) in a subset of patients. To date, which cell types mediate this effect and whether NAD results from on-target mechanisms is unknown. We utilized human induced pluripotent stem cells (hiPSCs) to generate endothelial cells and vascular smooth muscle cells for in vitro study of NAD. We found that nilotinib adversely affects endothelial proliferation and migration, in addition to increasing intracellular nitric oxide...
March 26, 2024: Scientific Reports
https://read.qxmd.com/read/38532089/quantitative-analysis-of-epigenetic-modifications-in-fagopyrum-nuclei-with-confocal-microscope-imagej-and-r-studio
#17
JOURNAL ARTICLE
Alicja Tomasiak, Lea Sophie Berg, Katarzyna Sala, Agnieszka Braszewska
Epigenetic programming plays a vital role in regulating pluripotency genes, which become activated or inactivated during the processes of dedifferentiation and differentiation during an organism's development. The analysis of epigenetic modifications has become possible through the technique of immunostaining, where specific antibodies allow the identification of a single target protein. This chapter describes a detailed protocol for the analysis of the epigenetic modifications with the use of confocal microscopy, subsequent image, and statistical analysis on the example of Fagopyrum calli with the use of nine antibodies raised against histone H3 and H4 methylation and acetylation on several lysines as well as DNA methylation...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38532070/generating-human-bone-marrow-organoids-for-disease-modeling-and-drug-discovery
#18
REVIEW
Aude-Anais Olijnik, Antonio Rodriguez-Romera, Zoë C Wong, Yuqi Shen, Jasmeet S Reyat, Natalie J Jooss, Julie Rayes, Bethan Psaila, Abdullah O Khan
The bone marrow supports and regulates hematopoiesis, responding to physiological requirements for blood cell production over ontogeny and during pathological challenges. Interactions between hematopoietic cells and niche components are challenging to study mechanistically in the human context, but are important to delineate in order to explore the pathobiology of blood and bone marrow disorders. Organoids are proving transformative in many research settings, but an accurate human bone marrow model incorporating multiple hematopoietic and stromal elements has been lacking...
March 26, 2024: Nature Protocols
https://read.qxmd.com/read/38532010/serotonin-effects-on-human-ipsc-derived-neural-cell-functions-from-mitochondria-to-depression
#19
JOURNAL ARTICLE
Iseline Cardon, Sonja Grobecker, Frederike Jenne, Tatjana Jahner, Rainer Rupprecht, Vladimir M Milenkovic, Christian H Wetzel
Depression's link to serotonin dysregulation is well-known. The monoamine theory posits that depression results from impaired serotonin activity, leading to the development of antidepressants targeting serotonin levels. However, their limited efficacy suggests a more complex cause. Recent studies highlight mitochondria as key players in depression's pathophysiology. Mounting evidence indicates that mitochondrial dysfunction significantly correlates with major depressive disorder (MDD), underscoring its pivotal role in depression...
March 26, 2024: Molecular Psychiatry
https://read.qxmd.com/read/38531866/noninvasive-assessment-of-hipsc-differentiation-toward-cardiomyocytes-using-pretrained-convolutional-neural-networks-and-the-channel-pruning-algorithm
#20
JOURNAL ARTICLE
Yujie Duan, Kaitong He, Wei Lan, Yuli Luo, Hao Fan, Peiran Lin, Wenlong Wang, Yadong Tang
Human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hiPSC-CMs) offer versatile applications in tissue engineering and drug screening. To facilitate the monitoring of hiPSC cardiac differentiation, a noninvasive approach using convolutional neural networks (CNNs) was explored. HiPSCs were differentiated into cardiomyocytes and analyzed using the quantitative real-time polymerase chain reaction (qRT-PCR). The bright-field images of the cells at different time points were captured to create the dataset...
March 26, 2024: ACS Biomaterials Science & Engineering
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