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https://www.readbyqxmd.com/read/29684900/generation-of-the-human-induced-pluripotent-stem-cell-hipsc-line-psmi003-a-from-a-patient-affected-by-an-autosomal-recessive-form-of-long-qt-syndrome-type-1
#1
Manuela Mura, Monia Ginevrino, Rita Zappatore, Federica Pisano, Marina Boni, Silvia Castelletti, Lia Crotti, Enza Maria Valente, Peter J Schwartz, Massimiliano Gnecchi
We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a 51years old female patient homozygous for the mutation c.535 G>A p.G179S on the KCNQ1 gene, causing a severe form of autosomal recessive Long QT Syndrome type 1 (AR-LQT1), not associated with deafness. The hiPSCs, generated using four retroviruses each encoding for a reprogramming factor OCT4, SOX2, KLF4, cMYC, are pluripotent and can differentiate into spontaneously beating cardiomyocytes (hiPSC-CMs).
April 6, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29684809/simultaneous-effects-of-igf1-and-fadrozole-on-parthenogenesis-and-pluripotency-markers-in-chicken-embryo
#2
Zohreh Hajihoseini, Shahin Eghbalsaied
So far, a synergistic effect was detected between insulin-like growth factor-I (IGF1) and anti-aromatase for sex reversal and pre-/post-natal growth of fertilized chicken embryo. Here, we hypothesized whether the growth and sexual female-to-male reversal effects of IGF1 and an anti-aromatase, Fadrozole, could improve the development of unfertilized, parthenogenetic chicken embryos. Simultaneous administration of IGF1 and Fadrozole increased the percentage of grade A embryos from 1.7% (no injection group) to 70...
April 13, 2018: Theriogenology
https://www.readbyqxmd.com/read/29684521/the-hypoxic-peri-arteriolar-glioma-stem-cell-niche-an-integrated-concept-of-five-types-of-niches-in-human-glioblastoma
#3
REVIEW
Diana A Aderetti, Vashendriya V V Hira, Remco J Molenaar, Cornelis J F van Noorden
Glioblastoma is the most lethal primary brain tumor and poor survival of glioblastoma patients is attributed to the presence of glioma stem cells (GSCs). These therapy-resistant, quiescent and pluripotent cells reside in GSC niches, which are specific microenvironments that protect GSCs against radiotherapy and chemotherapy. We previously showed the existence of hypoxic peri-arteriolar GSC niches in glioblastoma tumor samples. However, other studies have described peri-vascular niches, peri-hypoxic niches, peri-immune niches and extracellular matrix niches of GSCs...
April 20, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29684447/type-3-iodothyronine-deiodinase-is-expressed-in-human-induced-pluripotent-stem-cell-derived-cardiomyocytes
#4
Kumiko Nishimura, Masafumi Takeda, Jun K Yamashita, Ichiro Shiojima, Nagaoki Toyoda
AIMS: Type 3 iodothyronine deiodinase (D3), which converts thyroxine (T4 ) and 3,5,3'-triiodothyronine (T3 ) to 3,3',5'-triiodothyronine (rT3 ) and 3,3'-diiodothyronine (T2 ), respectively, inactivates thyroid hormones. We investigated the expression and regulation of D3 in human cardiomyocytes which were differentiated from human induced pluripotent stem cells (hiPSCs). MAIN METHODS: We characterized D3 activity using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis...
April 20, 2018: Life Sciences
https://www.readbyqxmd.com/read/29681920/combination-of-heterologous-fibrin-sealant-and-bioengineered-human-embryonic-stem-cells-to-improve-regeneration-following-autogenous-sciatic-nerve-grafting-repair
#5
Roghayeh Mozafari, Sergiy Kyrylenko, Mateus Vidigal Castro, Rui Seabra Ferreira, Benedito Barraviera, Alexandre Leite Rodrigues Oliveira
Background: Peripheral nerve injury is a worldwide clinical problem, and the preferred surgical method for treating it is the end-to-end neurorrhaphy. When it is not possible due to a large nerve gap, autologous nerve grafting is used. However, these surgical techniques result in nerve regeneration at highly variable degrees. It is thus very important to seek complementary techniques to improve motor and sensory recovery. One promising approach could be cell therapy. Transplantation therapy with human embryonic stem cells (hESCs) is appealing because these cells are pluripotent and can differentiate into specialized cell types and have self-renewal ability...
2018: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/29681545/nf-%C3%AE%C2%BAb-activity-initiates-human-esc-derived-neural-progenitor-cell-differentiation-by-inducing-a-metabolic-maturation-program
#6
Lorna M FitzPatrick, Kate E Hawkins, Juliette M K M Delhove, Emilio Fernandez, Chiara Soldati, Louise F Bullen, Axel Nohturfft, Simon N Waddington, Diego L Medina, Juan P Bolaños, Tristan R McKay
Human neural development begins at embryonic day 19 and marks the beginning of organogenesis. Neural stem cells in the neural tube undergo profound functional, morphological, and metabolic changes during neural specification, coordinated by a combination of exogenous and endogenous cues. The temporal cell signaling activities that mediate this process, during development and in the postnatal brain, are incompletely understood. We have applied gene expression studies and transcription factor-activated reporter lentiviruses during in vitro neural specification of human pluripotent stem cells...
April 17, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29681544/dissecting-the-contributions-of-cooperating-gene-mutations-to-cancer-phenotypes-and-drug-responses-with-patient-derived-ipscs
#7
Chan-Jung Chang, Andriana G Kotini, Malgorzata Olszewska, Maria Georgomanoli, Julie Teruya-Feldstein, Henrik Sperber, Roberto Sanchez, Robert DeVita, Timothy J Martins, Omar Abdel-Wahab, Robert K Bradley, Eirini P Papapetrou
Connecting specific cancer genotypes with phenotypes and drug responses constitutes the central premise of precision oncology but is hindered by the genetic complexity and heterogeneity of primary cancer cells. Here, we use patient-derived induced pluripotent stem cells (iPSCs) and CRISPR/Cas9 genome editing to dissect the individual contributions of two recurrent genetic lesions, the splicing factor SRSF2 P95L mutation and the chromosome 7q deletion, to the development of myeloid malignancy. Using a comprehensive panel of isogenic iPSCs-with none, one, or both genetic lesions-we characterize their relative phenotypic contributions and identify drug sensitivities specific to each one through a candidate drug approach and an unbiased large-scale small-molecule screen...
April 17, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29681541/differentiation-of-human-pluripotent-stem-cells-into-functional-endothelial-cells-in-scalable-suspension-culture
#8
Ruth Olmer, Lena Engels, Abdulai Usman, Sandra Menke, Muhammad Nasir Hayat Malik, Frank Pessler, Gudrun Göhring, Dorothee Bornhorst, Svenja Bolten, Salim Abdelilah-Seyfried, Thomas Scheper, Henning Kempf, Robert Zweigerdt, Ulrich Martin
Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers...
April 17, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29681539/x-chromosome-dosage-influences-dna-methylation-dynamics-during-reprogramming-to-mouse-ipscs
#9
Vincent Pasque, Rahul Karnik, Constantinos Chronis, Paula Petrella, Justin Langerman, Giancarlo Bonora, Juan Song, Lotte Vanheer, Anupama Sadhu Dimashkie, Alexander Meissner, Kathrin Plath
A dramatic difference in global DNA methylation between male and female cells characterizes mouse embryonic stem cells (ESCs), unlike somatic cells. We analyzed DNA methylation changes during reprogramming of male and female somatic cells and in resulting induced pluripotent stem cells (iPSCs). At an intermediate reprogramming stage, somatic and pluripotency enhancers are targeted for partial methylation and demethylation. Demethylation within pluripotency enhancers often occurs at ESC binding sites of pluripotency transcription factors...
April 14, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29681516/super-obese-patient-derived-ipsc-hypothalamic-neurons-exhibit-obesogenic-signatures-and-hormone-responses
#10
Uthra Rajamani, Andrew R Gross, Brooke E Hjelm, Adolfo Sequeira, Marquis P Vawter, Jie Tang, Vineela Gangalapudi, Yizhou Wang, Allen M Andres, Roberta A Gottlieb, Dhruv Sareen
The hypothalamus contains neurons that integrate hunger and satiety endocrine signals from the periphery and are implicated in the pathophysiology of obesity. The limited availability of human hypothalamic neurons hampers our understanding of obesity disease mechanisms. To address this, we generated human induced pluripotent stem cells (hiPSCs) from multiple normal body mass index (BMI; BMI ≤ 25) subjects and super-obese (OBS) donors (BMI ≥ 50) with polygenic coding variants in obesity-associated genes...
April 6, 2018: Cell Stem Cell
https://www.readbyqxmd.com/read/29681444/high-cell-density-suppresses-bmp4-induced-differentiation-of-human-pluripotent-stem-cells-to-produce-macroscopic-spatial-patterning-in-a-unidirectional-perfusion-culture-chamber
#11
Shota Tashiro, Minh Nguyen Tuyet Le, Yuta Kusama, Eri Nakatani, Mika Suga, Miho K Furue, Taku Satoh, Shinji Sugiura, Toshiyuki Kanamori, Kiyoshi Ohnuma
Spatial pattern formation is a critical step in embryogenesis. Bone morphogenetic protein 4 (BMP4) and its inhibitors are major factors for the formation of spatial patterns during embryogenesis. However, spatial patterning of the human embryo is unclear because of ethical issues and isotropic culture environments resulting from conventional culture dishes. Here, we utilized human pluripotent stem cells (hiPSCs) and a simple anisotropic (unidirectional perfusion) culture chamber, which creates unidirectional conditions, to measure the cell community effect...
April 19, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29680945/cilostazol-is-effective-to-prevent-stroke-associated-pneumonia-in-patients-receiving-tube-feeding
#12
Shizuka Netsu, Atsushi Mizuma, Masaki Sakamoto, Sachiko Yutani, Eiichiro Nagata, Shunya Takizawa
Stroke-associated pneumonia (SAP) is a frequent complication in acute ischemic stroke (IS) patients, especially those receiving tube feeding (TF). In this retrospective study, we investigated whether or not cilostazol, a pluripotent phosphodiesterase III-specific inhibitor with anti-platelet and vasculogenic effects, can prevent SAP in these patients and reduce their duration of stay in intensive care unit/hospitalization. We recruited 158 IS patients receiving TF. Patients' characteristics (including age, gender, past history), National Institute of Health Stroke Scale and serum albumin level on admission, concomitant medications associated with SAP prevention (including cilostazol), and stroke characteristics (bilateral subcortical white matter lesion, brainstem involvement, large infarction, and asymptomatic hemorrhagic infarction) were compared between the SAP(-) and SAP(+) groups...
April 21, 2018: Dysphagia
https://www.readbyqxmd.com/read/29679845/establishment-of-stub1-chip-mutant-induced-pluripotent-stem-cells-ipscs-from-a-patient-with-gordon-holmes-syndrome-scar16
#13
Stefanie Schuster, Yvonne Schelling, Matthis Synofzik, Philip Höflinger, Ludger Schöls, Stefan Hauser
STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Here, we reprogrammed human skin fibroblasts from a 12-year-old male patient with recessive spinocerebellar ataxia type 16 (OMIM #615768), carrying compound heterozygous mutations (c.355C>T, c.880A>T) in STUB1. Genomic integrity of the iPSC line HIHCNi001-A without transgene integration and genomic aberration but with maintained disease-relevant mutations was proven by SNP array analysis and Sanger sequencing while pluripotency was verified by the expression of important pluripotency markers and the capacity to differentiate into cells of all three germ layers...
April 9, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29679475/chemically-controlled-aggregation-of-pluripotent-stem-cells
#14
Yonatan Y Lipsitz, Peter Tonge, Peter W Zandstra
Heterogeneity in pluripotent stem cell (PSC) aggregation leads to variability in mass transfer and signaling gradients between aggregates, which results in heterogeneous differentiation and therefore variability in product quality and yield. We have characterized a chemical based method to control aggregate size within a specific, tunable range with low heterogeneity, thereby reducing process variability in PSC expansion. This method enables controlled, scalable, stirred suspension based manufacturing of PSC cultures which are critical for the translation of regenerative medicine strategies to clinical products...
April 21, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29678882/mesenchyme-homeobox-1-mediates-transforming-growth-factor-%C3%AE-tgf-%C3%AE-induced-smooth-muscle-cell-differentiation-from-mouse-mesenchymal-progenitors
#15
Kun Dong, Xia Guo, Weiping Chen, Amanda C Hsu, Qiang Shao, Jian-Fu Chen, Shi-You Chen
Differentiation of smooth muscle cells (SMCs) is critical for proper vasculogenesis and angiogenesis. However, the molecular mechanisms controlling SMC differentiation are not completely understood. During embryogenesis, the transcription factor mesenchyme homeobox 1 (Meox1) is expressed in the early developing somite, which is one of the origins of SMCs. In the present study, we identified Meox1 as a positive regulator of SMC differentiation. We found that transforming growth factor-β (TGF-β) induces Meox1 expression in the initial phase of SMC differentiation of pluripotent murine C3H10T1/2 cells...
April 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29678834/from-pluripotent-stem-to-car-t-cells
#16
(no author information available yet)
Within the field of allogeneic chimeric antigen receptor T-cell therapy, La Jolla, CA-based Fate Therapeutics is exploring in vitro differentiation of induced pluripotent stem cells, rather than T cells harvested from donors, as their source material. Preclinical data on the company's off-the-shelf candidate, FT819, look promising so far.
April 20, 2018: Cancer Discovery
https://www.readbyqxmd.com/read/29677590/generation-of-a-human-induced-pluripotent-stem-cell-line-from-urinary-cells-of-a-patient-with-primary-congenital-glaucoma-using-integration-free-sendai-technology
#17
Jingxue Zhang, Shen Wu, Man Hu, Qian Liu
We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of a 10years old patient with primary congenital glaucoma (PCG). The cells were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system and shown to have full differentiation potential. The line is available and registered in the human pluripotent stem cell registry as BIOi001-A.
April 9, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29677589/generation-of-the-human-induced-pluripotent-stem-cell-hipsc-line-psmi002-a-from-a-patient-affected-by-the-jervell-and-lange-nielsen-syndrome-and-carrier-of-two-compound-heterozygous-mutations-on-the-kcnq1-gene
#18
Manuela Mura, Yee-Ki Lee, Monia Ginevrino, Rita Zappatore, Federica Pisano, Marina Boni, Federica Dagradi, Lia Crotti, Enza Maria Valente, Peter J Schwartz, Hung-Fat Tse, Massimiliano Gnecchi
We report the generation of human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a female patient carrier of the two compound heterozygous mutations c.568 C>T p.R190W (maternal allele), and c.1781 G>A p.R594Q (paternal allele) on the KCNQ1 gene, causing Jervell and Lange-Nielsen Syndrome (JLNS). To obtain hiPSCs, we used the classical approach of the four retroviruses each encoding for a reprogramming factor OCT4, SOX2, KLF4, cMYC. The obtained hiPSC clones display pluripotent stem cell characteristics, and differentiate into spontaneously beating cardiomyocytes (hiPSC-CMs)...
April 7, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29677519/suspended-graphene-oxide-nanosheets-maintain-the-self-renewal-of-mouse-embryonic-stem-cells-via-down-regulating-the-expression-of-vinculin
#19
Guoxin Jing, Zhaojie Wang, Xizhen Zhuang, Xiaolie He, Huijun Wu, Qingxiu Wang, Liming Cheng, Zhongmin Liu, Shilong Wang, Rongrong Zhu
Graphene oxide (GO), with good hydrophilicity and biocompatibility, is widely explored as a carrier for various factors in the field of stem cell differentiation. However, its function of sustaining the stemness of mouse embryonic stem cells (mESCs) and the underlying mechanisms of this process remains undiscovered. Herein, we explored the biofunction of GO on mESCs and revealed the involved signaling pathways and key gene. The alkaline phosphatase activity detection, pluripotency genes quantification and the teratomas formation in vivo confirmed that GO nanosheets could sustain the self-renewal ability of mESCs instead of influencing its pluripotency...
April 11, 2018: Biomaterials
https://www.readbyqxmd.com/read/29676650/the-pluripotency-factor-musashi-2-is-a-novel-target-for-lung-cancer-therapy
#20
Mary R Zhang, Sichuan Xi, Vivek Shukla, Julie A Hong, Haobin Chen, Yin Xiong, R Taylor Ripley, Chuong D Hoang, David S Schrump
Previously we reported that mithramycin represses multiple pathways critical for stem cell signaling and pluripotency in lung cancer cells. This phenomenon coincided with decreased side population fraction and dramatic dose-dependent growth arrest of lung cancer cells in vitro and in vivo. In the present study, microarray, quantitative reverse transcriptase polymerase chain reaction and immunoblot experiments were performed to further examine the effects of mithramycin on stem cell gene expression in lung cancer cells...
April 2018: Annals of the American Thoracic Society
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