Fang-fang Wen, Li-ming Zheng, Xiao-jing Li, Yan Li, Lei Zhang, Xue-mei Cheng, Chang-hong Wang, Zheng-taou Wang
OBJECTIVE: To establish the quality standards of the herbs of Peganum harmala. METHOD: According to the Chinese Pharmacopoeia (2010 version, volume 1) and its appendix method, the water, total ash, acid insoluble ash, water-soluble extractives, and heavy metal were analyzed for herbs of P. harmala. TLC method was used to separate harmaline, harmine and vasicine from the herb samples by mixture of ethyl acetate-methanol-ammonia (10: 1.5: 0.5) as a developing solvent on high performance silica gel precoated plate (HSGF254) and to identify them inspected under UV 366 nm, visualized by spraying with both Dragendorff reagent, and by bioautographic assay...
October 2012: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica