keyword
MENU ▼
Read by QxMD icon Read
search

Zonula occluden-1

keyword
https://www.readbyqxmd.com/read/29777753/characterization-of-impaired-cerebrovascular-structure-in-app-ps1-mouse-brains
#1
Kee-Chan Ahn, Cameron R Learman, Gary L Dunbar, Panchanan Maiti, Won-Cheoul Jang, Hyeon-Cheol Cha, Mee-Sook Song
Alzheimer's disease (AD) is defined by senile plaques, tauopathy and neuronal cell death in specific area of the brain. Recent studies suggest that neurovascular dysfunction may be an integral part of AD pathogenesis, contributing to the onset and development of AD pathologies such as neuronal death, inflammatory response, and breakdown of blood brain barrier (BBB). In addition, vascular complications caused by age-related metabolic diseases such as diabetes and high blood pressure have high incidence in development of dementia and AD...
May 16, 2018: Neuroscience
https://www.readbyqxmd.com/read/29776350/increased-systemic-zonula-occludens-1-associated-with-inflammation-and-independent-biomarker-in-patients-with-hepatocellular-carcinoma
#2
Amit Kumar Ram, Biju Pottakat, Balasubramaniyan Vairappan
BACKGROUND: Hepatocellular carcinoma (HCC) is a frequent type of primary liver cancer, and its prevalence is increasing worldwide. Indeed, the underlying molecular mechanism is not well understood. Previous studies have shown evidence that tight junction (TJ) components were correlated with carcinogenesis and tumor development. Our aims were to determine the serum levels of tight junction protein Zonula Occludens (ZO)-1 and an inflammatory marker such as high-sensitive C-reactive protein (hs-CRP) in HCC patients compared to healthy volunteers and also to identify the association between ZO-1 and inflammation in HCC...
May 18, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29775658/hydroxysafflor-yellow-a-protects-brain-microvascular-endothelial-cells-against-oxygen-glucose-deprivation-reoxygenation-injury-involvement-of-inhibiting-autophagy-via-class-i-pi3k-akt-mtor-signaling-pathway
#3
Guang Yang, Ning Wang, Sai Wang Seto, Dennis Chang, Huazheng Liang
The present study aimed to test whether Hydroxysafflor yellow A (HSYA) protects the brain microvascular endothelial cells (BMECs) injury induced by oxygen glucose deprivation/reoxygenation (OGD/R) via the PI3K/Akt/mTOR autophagy signaling pathway. Primary rat BMECs were cultured and identified by the expression of factor VIII-related antigen before being exposed to OGD/R to imitate ischemia/reperfusion (I/R) damage in vitro. The protective effect of HSYA was evaluated by assessing (1) cellular morphologic and ultrastructural changes; (2) cell viability and cytotoxicity; (3) transendothelial electrical resistance (TEER) of monolayer BMECs; (4) cell apoptosis; (5) fluorescence intensity of LC3B; (6) LC3 mRNA expression; (7) protein expressions of LC3, Beclin-1, Zonula occludens-1 (ZO-1), phospho-Akt (p-Akt), Akt, phospho-mTOR (p-mTOR) and mTOR...
May 15, 2018: Brain Research Bulletin
https://www.readbyqxmd.com/read/29771433/microrna-23a-3p-promotes-the-perihematomal-edema-formation-after-intracerebral-hemorrhage-via-zo-1
#4
Y-L Hu, H Wang, Q Huang, G Wang, H-B Zhang
OBJECTIVE: To explore the relationship between microRNA-23a-3p expression and perihematomal edema (PHE) in patients with acute intracerebral hemorrhage (ICH) and its underlying mechanism. PATIENTS AND METHODS: Clinical data and blood samples on the 3rd day after ICH onset were collected. Head CT was performed in each subject when admitted. Serum expressions of microRNAs were detected by quantitative real-time PCR (qRT-PCR). The relationship between hematoma volume and perihematomal edema was analyzed by correlation analysis...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29770758/dietary-l-threonine-supplementation-attenuates-lipopolysaccharide-induced-inflammatory-responses-and-intestinal-barrier-damage-of-broiler-chickens-at-an-early-age
#5
Yueping Chen, Hao Zhang, Yefei Cheng, Yue Li, Chao Wen, Yanmin Zhou
This study was conducted to investigate the protective effects of l-threonine (l-Thr) supplementation on growth performance, inflammatory responses and intestinal barrier function of young broilers challenged with lipopolysaccharide (LPS). A total of 144 1-d-old male chicks were allocated to one of three treatments: non-challenged broilers fed a basal diet (control group), LPS-challenged broilers fed a basal diet without l-Thr supplementation and LPS-challenged broilers fed a basal diet supplemented with 3·0 g/kg l-Thr...
June 2018: British Journal of Nutrition
https://www.readbyqxmd.com/read/29768670/patients-affected-by-a-new-variant-of-endemic-pemphigus-foliaceus-have-autoantibodies-colocalizing-with-myzap-p0071-desmoplakins-1-2-and-arvcf-causing-renal-damage
#6
A M Abreu-Velez, M S Howard, H Yi, A A Florez-Vargas
BACKGROUND: We have previously reported that about 30% of patients affected by a new variant of endemic pemphigus foliaceus (EPF) in El Bagre, Colombia (termed El Bagre-EPF or pemphigus Abreu-Manu) have systemic compromise. In the current study, we focused on studying autoreactivity to the kidney and its pathological correlations. AIM: To investigate patients with El Bagre-EPF for renal compromise. METHODS: We performed a case-control study, enrolling 57 patients with El Bagre-EPF and 57 controls from the endemic area, matched by age, sex, race, work activity, demographics and comorbidities...
May 16, 2018: Clinical and Experimental Dermatology
https://www.readbyqxmd.com/read/29767267/hdac-inhibitors-trichostatin-a-and-valproic-acid-increase-e%C3%A2-cadherin-and-vimentin-expression-but-inhibit-migration-and-invasion-of-cholangiocarcinoma-cells
#7
Joon Ho Wang, Eun Jeoung Lee, Meiying Ji, Seon Mee Park
The effects of histone deacetylase (HDAC) inhibitors on epithelial-mesenchymal transition (EMT) differ in various cancers. E‑cadherin is a cell‑to‑cell adhesion protein, whereas accumulation of vimentin is related to the development of the spindle shape of the mesenchymal cell phenotype. We investigated the EMT phenotypes of human cholangiocellular carcinoma HuCC‑T1, JCK and SNU‑1079 cell lines. To this end, we measured the expression of E‑cadherin or zonula occludens (ZO)‑1 and vimentin, epithelial and mesenchymal cell markers, respectively, using real‑time reverse transcription‑polymerase chain reaction, western blotting, and immunofluorescence microscopy following treatment with trichostatin A (TSA, 200 nM) or valproic acid (VPA, 0...
May 16, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29765546/proteomic-screening-identifies-the-zonula-occludens-protein-zo-1-as-a-new-partner-for-adam12-in-invadopodia-like-structures
#8
Bassil Dekky, Michael Ruff, Dominique Bonnier, Vincent Legagneux, Nathalie Théret
The epithelial mesenchymal transition (EMT) is a key process for cancer cell invasion and migration. This complex program whereby epithelial tumor cells loose polarity and acquire mesenchymal phenotype is driven by the regulation of cell-cell adhesion and cell-substrate interactions. We recently described the association of ADAM12 with EMT and we now use immunoprecipitation and proteomic approaches to identify interacting partners for ADAM12 during EMT. We identify twenty proteins that are involved in molecular mechanisms associated with adhesion/invasion processes...
April 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29749551/angiotensin-ii-inhibits-the-protein-expression-of-zo%C3%A2-1-in-vascular-endothelial-cells-by-downregulating-ve%C3%A2-cadherin
#9
Longbin Liu, Liping Meng, Peng Zhang, Hui Lin, Jufang Chi, Fang Peng, Hangyuan Guo
Angiotensin II (Ang II) is reported to be involved in the development of various cardiovascular diseases by disrupting microvessel permeability, however, the underlying mechanism remains to be elucidated. The present study aimed to investigate the mechanism by which Ang II disrupts microvascular permeability. Rat endothelial cells were subjected to primary culture and identification. Cells in passages 4‑7 were then used for the following experiments. The cells were divided into control, Ang II, and Ang II + valsartan groups, and reverse transcription‑quantitative polymerase chain reaction and western blot analyses were perform to evaluate the expression of zonula occludens‑1 (ZO‑1) and vascular endothelial (VE)‑cadherin in the cells...
May 8, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29740204/oral-treatment-with-plecanatide-or-dolcanatide-attenuates-visceral-hypersensitivity-via-activation-of-guanylate-cyclase-c-in-rat-models
#10
Illona-Marie Boulete, Anusha Thadi, Catherine Beaufrand, Viren Patwa, Apoorva Joshi, John A Foss, E Priya Eddy, Helene Eutamene, Vaseem A Palejwala, Vassilia Theodorou, Kunwar Shailubhai
AIM: To investigate the effects of plecanatide and dolcanatide on maintenance of paracellular permeability, integrity of tight junctions and on suppression of visceral hypersensitivity. METHODS: Transport of fluorescein isothiocyanate (FITC)-dextran was measured to assess permeability across cell monolayers and rat colon tissues. Effects of plecanatide and dolcanatide on the integrity of tight junctions in Caco-2 and T84 monolayers and on the expression and localization of occludin and zonula occludens-1 (ZO-1) were examined by immunofluorescence microscopy...
May 7, 2018: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29719591/acidic-bile-salts-induces-mucosal-barrier-dysfunction-through-let-7a-reduction-during-gastric-carcinogenesis-after-helicobacter-pylori-eradication
#11
Yasushi Takahashi, Kaname Uno, Katsunori Iijima, Yasuhiko Abe, Tomoyuki Koike, Naoki Asano, Kiyotaka Asanuma, Tooru Shimosegawa
Gastric cancer (GC) after eradication for Helicobacter pylori ( H.pylori ) increases, but its carcinogenesis is not elucidated. It is mainly found in acid non-secretion areas (ANA), as mucosal regeneration in acid secretory areas (AA) after eradication changes the acidity and bile toxicity of gastric juice. We aimed to clarify the role of barrier dysfunction of ANA by the stimulation of pH3 bile acid cocktail (ABC) during carcinogenesis. We collected 18 patients after curative endoscopic resection for GC, identified later than 24 months after eradication, and took biopsies by Congo-red chromoendoscopy to distinguish AA and ANA (UMIN00018967)...
April 6, 2018: Oncotarget
https://www.readbyqxmd.com/read/29713894/fluoxetine-is-neuroprotective-in-early-brain-injury-via-its-anti-inflammatory-and-anti-apoptotic-effects-in-a-rat-experimental-subarachnoid-hemorrhage-model
#12
Hui-Min Hu, Bin Li, Xiao-Dong Wang, Yun-Shan Guo, Hua Hui, Hai-Ping Zhang, Biao Wang, Da-Geng Huang, Ding-Jun Hao
Fluoxetine, an anti-depressant drug, has recently been shown to provide neuroprotection in central nervous system injury, but its roles in subarachnoid hemorrhage (SAH) remain unclear. In this study, we aimed to evaluate whether fluoxetine attenuates early brain injury (EBI) after SAH. We demonstrated that intraperitoneal injection of fluoxetine (10 mg/kg per day) significantly attenuated brain edema and blood-brain barrier (BBB) disruption, microglial activation, and neuronal apoptosis in EBI after experimental SAH, as evidenced by the reduction of brain water content and Evans blue dye extravasation, prevention of disruption of the tight junction proteins zonula occludens-1, claudin-5, and occludin, a decrease of cells staining positive for Iba-1, ED-1, and TUNEL and a decline in IL-1β, IL-6, TNF-α, MDA, 3-nitrotyrosine, and 8-OHDG levels...
April 30, 2018: Neuroscience Bulletin
https://www.readbyqxmd.com/read/29713228/blood-brain-barrier-disruption-and-perivascular-beta-amyloid-accumulation-in-the-brain-of-aged-rats-with-spontaneous-hypertension-evaluation-with-dynamic-contrast-enhanced-magnetic-resonance-imaging
#13
Yu Wang, Ruzhi Zhang, Chuanyuan Tao, Ziqian Xu, Wei Chen, Chunhua Wang, Li Song, Jie Zheng, Fabao Gao
Objective: Whether blood-brain barrier (BBB) disruption induced by chronic spontaneous hypertension is associated with beta-amyloid (Aβ) accumulation in the brain remains poorly understood. The purpose of this study was to investigate the relationship between BBB disruption and Aβ influx and accumulation in the brain of aged rats with chronic spontaneous hypertension. Materials and Methods: Five aged spontaneously hypertensive rats (SHRs) and five age-matched normotensive Wistar-Kyoto (WKY) rats were studied...
May 2018: Korean Journal of Radiology: Official Journal of the Korean Radiological Society
https://www.readbyqxmd.com/read/29702245/female-mice-carrying-a-defective-alox15-gene-are-protected-from-experimental-colitis-via-sustained-maintenance-of-the-intestinal-epithelial-barrier-function
#14
Saskia Kroschwald, Cheng-Ying Chiu, Dagmar Heydeck, Nadine Rohwer, Tatjana Gehring, Ulrike Seifert, Anke Lux, Michael Rothe, Karsten-Henrich Weylandt, Hartmut Kuhn
Lipoxygenases (ALOX) are involved in the regulation of cellular redox homeostasis. They also have been implicated in the biosynthesis of pro- and anti-inflammatory lipid mediators and play a role in the pathogenesis of inflammatory diseases, which constitute a major health challenge owing to increasing incidence and prevalence in all industrialized countries around the world. To explore the pathophysiological role of Alox15 (leukocyte-type 12-LOX) in mouse experimental colitis we tested the impact of systemic inactivation of the Alox15 gene on the extent of dextrane sulfate sodium (DSS) colitis...
April 24, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29690739/-effects-of-short-chain-fatty-acid-on-barrier-disruption-of-human-intestinal-epithelial-cell-induced-by-endotoxin-lipopolysaccharide-and-the-related-mechanism
#15
Y H Feng, Y L Huang, P Wang, F J Wang
Objective: To investigate the effects of short chain fatty acid (SCFA) on barrier disruption of human intestinal epithelial cell induced by endotoxin/lipopolysaccharide (LPS) and the related mechanism. Methods: The human intestinal epithelial cell line Caco-2 was used to reproduce monolayer-cells. Cells were divided into control group, LPS group, and SCFA+ LPS group according to the random number table. Cells in control group were only routinely cultured with DMEM medium. Cells in LPS group were cultured with DMEM medium and LPS with final mass concentration of 10 μg/mL...
April 20, 2018: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/29680447/cultivation-of-corneal-endothelial-cells-from-sheep
#16
Najla Khaled Al Abdulsalam, Nigel L Barnett, Damien G Harkin, Jennifer Walshe
Research is currently under way to produce tissue engineered corneal endothelium transplants for therapeutic use in humans. This work requires the use of model animals, both for the supply of corneal endothelial cells (CECs) for experimentation, and to serve as recipients for test transplants. A variety of species can be used, however, a number of important advantages can be gained by using sheep as transplant recipients. The purpose of the present study was therefore to develop a method for culturing sheep CECs that would be suitable for the eventual construction of corneal endothelium grafts destined for sheep subjects...
April 19, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29679061/the-role-of-macrophage-migration-inhibitory-factor-in-the-function-of-intestinal-barrier
#17
Milica Vujicic, Tamara Saksida, Sanja Despotovic, Svetlana Sokovic Bajic, Ivana Lalić, Ivan Koprivica, Dragica Gajic, Natasa Golic, Maja Tolinacki, Ivana Stojanovic
Macrophage migration inhibitory factor (MIF) is a multifunctional protein that is involved in the development of gut-related inflammation. To investigate the role of MIF in the function of the intestinal barrier, we have explored intestinal permeability and gut-associated immune response in MIF-deficient (MIF-KO) mice. The absence of MIF provoked impairment of tight and adherens epithelial junctions in the colon through the disturbance of E-cadherin, zonula occludens-1, occludin and claudin-2 expression, which lead to the increase of intestinal barrier permeability...
April 20, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29673258/methylglyoxal-disrupts-paranodal-axoglial-junctions-via-calpain-activation
#18
Ryan B Griggs, Leonid M Yermakov, Domenica E Drouet, Duc V M Nguyen, Keiichiro Susuki
Nodes of Ranvier and associated paranodal and juxtaparanodal domains along myelinated axons are essential for normal function of the peripheral and central nervous systems. Disruption of these domains as well as increases in the reactive carbonyl species methylglyoxal are implicated as a pathophysiology common to a wide variety of neurological diseases. Here, using an ex vivo nerve exposure model, we show that increasing methylglyoxal produces paranodal disruption, evidenced by disorganized immunostaining of axoglial cell-adhesion proteins, in both sciatic and optic nerves from wild-type mice...
January 2018: ASN Neuro
https://www.readbyqxmd.com/read/29661352/effects-of-alpinetin-on-intestinal-barrier-function-inflammation-and-oxidative-stress-in-dextran-sulfate-sodium-induced-ulcerative-colitis-mice
#19
Yue Tan, Changqing Zheng
BACKGROUND: Alpinetin is a flavonoid isolated from Alpinia katsumadai Hayata that has demonstrated anti-inflammatory, antibacterial and anti-tumor activities. However, alpinetin has not been widely studied in amelioration of inflammatory bowel disease. The study aimed to investigate the role of alpinetin on intestinal epithelial tight junctions, oxidative stress and Nrf2/HO-1 signaling pathway in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice. METHODS: A total of 40 mice were divided into 5 groups (n = 8/group): control group, DSS group (received 3% DSS), and low, medium and high-dose treatment groups (3% DSS + alpinetin 25, 50 and 100mg/kg)...
April 2018: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/29660533/role-of-lysophosphatidic-acid-in-the-retinal-pigment-epithelium-and-photoreceptors
#20
Grace E Lidgerwood, Andrew J Morris, Alison Conquest, Maciej Daniszewski, Louise A Rooney, Shiang Y Lim, Damián Hernández, Helena H Liang, Penelope Allen, Paul P Connell, Robyn H Guymer, Alex W Hewitt, Alice Pébay
The human retina is a complex structure of organised layers of specialised cells that support the transmission of light signals to the visual cortex. The outermost layer of the retina, the retinal pigment epithelium (RPE) forms part of the blood retina barrier and is implicated in many retinal diseases. Lysophosphatidic acid (LPA) is a bioactive lipid exerting pleiotropic effects in various cell types, during development, normal physiology and disease. Its producing enzyme AUTOTAXIN (ATX) is highly expressed by the pigmented epithelia of the human eye, including the RPE...
April 13, 2018: Biochimica et Biophysica Acta
keyword
keyword
46155
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"