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https://www.readbyqxmd.com/read/28339048/agr2-ameliorates-tumor-necrosis-factor-%C3%AE-induced-epithelial-barrier-dysfunction-via-suppression-of-nf-%C3%AE%C2%BAb-p65-mediated-mlck-p-mlc-pathway-activation
#1
Xiaolin Ye, Mei Sun
Intestinal epithelial barrier dysfunction plays a critical role in the pathogenesis of inflammatory bowel disease (IBD). Anterior gradient protein 2 homologue (AGR2) assists in maintaining intestinal homeostasis in dextran sulphate sodium-induced mouse ileocolitis; however, it is unclear whether it modulates intestinal barrier function. Our study aimed to investigate the protective role of AGR2 in tumor necrosis factor (TNF)-α-induced intestinal epithelial barrier injury. Caco-2 cell monolayers were pre-transfected with an AGR2 plasmid and then exposed to TNF-α...
March 21, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28333312/establishment-maintenance-and-functional-integrity-of-the-blood-testis-barrier-in-organotypic-cultures-of-fresh-and-frozen-thawed-prepubertal-mouse-testes
#2
C Rondanino, A Maouche, L Dumont, A Oblette, N Rives
STUDY QUESTION: Can the spatio-temporal formation of an intact blood-testis barrier (BTB), which is essential for the progression of spermatogenesis, be reproduced in cultures of fresh or frozen/thawed prepubertal mouse testes? SUMMARY ANSWER: Organotypic cultures allow the establishment and maintenance of major BTB components and the formation of a functional BTB in mouse testicular tissues. WHAT IS KNOWN ALREADY: In vitro maturation of prepubertal testicular tissues is a promising approach to restore fertility in adult survivors of childhood cancer...
March 17, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28328069/autophagy-maintains-the-integrity-of-endothelial-barrier-in-lps-induced-lung-injury
#3
Dan Zhang, Jian Zhou, Le Chi Ye, Jing Li, Zhenzhou Wu, Yuping Li, Chichi Li
Understanding the role and underlying regulation mechanism of autophagy in lipopolysaccharide-induced lung injury (LPS-LI) may provide potentially new pharmacological targets for treatment of acute lung injury. The aim of this study was to investigate the functional significance of autophagy in LPS-LI. The autophagy of human pulmonary microvascular endothelial cells (HPMVECs) and mice was inhibited before they were challenged with LPS. In vitro, permeability, vitality and the lactate dehydrogenase (LDH) release rate of the cells were detected, the zonula occluden-1 (ZO-1) expression and the stress fiber formation were determined...
March 22, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28323272/constipation-predominant-irritable-bowel-syndrome-females-have-normal-colonic-barrier-and-secretory-function
#4
Stephanie A Peters, Shoko Edogawa, Wendy J Sundt, Roy B Dyer, Daniel A Dalenberg, Amelia Mazzone, Ravinder J Singh, Natalie Moses, Thomas C Smyrk, Christopher Weber, David R Linden, Wallace K MacNaughton, Jerrold R Turner, Michael Camilleri, David A Katzka, Gianrico Farrugia, Madhusudan Grover
OBJECTIVES: The objective of this study was to determine whether constipation-predominant irritable bowel syndrome (IBS-C) is associated with changes in intestinal barrier and secretory function. METHODS: A total of 19 IBS-C patients and 18 healthy volunteers (all females) underwent saccharide excretion assay (0.1 g (13)C mannitol and 1 g lactulose), measurements of duodenal and colonic mucosal barrier (transmucosal resistance (TMR), macromolecular and Escherichia coli Bio-Particle translocation), mucosal secretion (basal and acetylcholine (Ach)-evoked short-circuit current (Isc)), in vivo duodenal mucosal impedance, circulating endotoxins, and colonic tight junction gene expression...
March 21, 2017: American Journal of Gastroenterology
https://www.readbyqxmd.com/read/28320523/roles-of-fluid-shear-stress-and-retinoic-acid-in-the-differentiation-of-primary-cultured-human-podocytes
#5
Seung Hee Yang, Jin Woo Choi, Dongeun Huh, Hyung Ah Jo, Sejoong Kim, Chun Soo Lim, Jung Chan Lee, Hee Chan Kim, Hyug Moo Kwon, Chang Wook Jeong, Cheol Kwak, Kwon Wook Joo, Yon Su Kim, Dong Ki Kim
Due to the distinct features that distinguish immortalized podocyte cell lines from their in vivo counterparts, primary cultured human podocytes might be a superior cell model for glomerular disease studies. However, the podocyte de-differentiation that occurs in culture remains an unresolved problem. Here, we present a method to differentiate primary cultured podocytes using retinoic acid (RA) and fluid shear stress (FSS), which mimic the in vivo environment of the glomerulus. RA treatment induced changes in the cell shape of podocytes from a cobblestone-like morphology to an arborized configuration with enhanced mobility...
March 16, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28303918/effects-of-matrix-stiffness-on-epithelial-to-mesenchymal-transition-like-processes-of-endometrial-epithelial-cells-implications-for-the-pathogenesis-of-endometriosis
#6
Sachiko Matsuzaki, Claude Darcha, Jean-Luc Pouly, Michel Canis
Endometriosis is defined as the presence of endometrial glands and stroma within extrauterine sites. Our previous study revealed an epithelial to mesenchymal transition (EMT)-like process in red peritoneal endometriosis, whereas membrane localization of E-cadherin was well maintained in epithelial cells of deep infiltrating endometriosis (DIE). Here we show that endometrial epithelial cells (EEE) grown on polyacrylamide gel substrates (PGS) of 2 kilopascal (kPa), a soft matrix, initiate a partial EMT-like process with transforming growth factor-β1 (TGF-β1) stimulation...
March 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28300333/psychological-stress-induced-colonic-barrier-dysfunction-role-of-immune-mediated-mechanisms
#7
P Hattay, D K Prusator, L Tran, B Greenwood-Van Meerveld
BACKGROUND: Evidence suggests that patients with irritable bowel syndrome (IBS) exhibit increases in gut permeability and alterations in tight junction (TJ) protein expression. Although psychological stress worsens IBS symptoms, the mechanisms by which stress enhances gut permeability and affects TJ protein expression remain to be determined. Here, we test the hypothesis that chronic intermittent psychological stress activates the release of proinflammatory cytokines to alter TJ proteins and promotes increased gut permeability...
March 16, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28287613/knockdown-of-long-non-coding-rna-xist-increases-blood-tumor-barrier-permeability-and-inhibits-glioma-angiogenesis-by-targeting-mir-137
#8
H Yu, Y Xue, P Wang, X Liu, J Ma, J Zheng, Z Li, Z Li, H Cai, Y Liu
Antiangiogenic therapy plays a significant role in combined glioma treatment. However, poor permeability of the blood-tumor barrier (BTB) limits the transport of chemotherapeutic agents, including antiangiogenic drugs, into tumor tissues. Long non-coding RNAs (lncRNAs) have been implicated in various diseases, especially malignant tumors. The present study found that lncRNA X-inactive-specific transcript (XIST) was upregulated in endothelial cells that were obtained in a BTB model in vitro. XIST knockdown increased BTB permeability and inhibited glioma angiogenesis...
March 13, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28279791/methionine-hydroxy-analogue-improves-intestinal-immunological-and-physical-barrier-function-in-young-grass-carp-ctenopharyngodon-idella
#9
Fei-Yu Pan, Pei Wu, Lin Feng, Wei-Dan Jiang, Sheng-Yao Kuang, Ling Tang, Wu-Neng Tang, Yong-An Zhang, Xiao-Qiu Zhou, Yang Liu
This study was conducted to test the hypothesis that methionine hydroxy analogue (MHA) enhances the defense against enteritis occurrence via improving intestinal barrier function in fish. After 630 young grass carp (Ctenopharyngodon idella) (259.70 ± 0.47 g) fed six graded levels of MHA (0, 2.4, 4.4, 6.4, 8.5 and 10.5 g/kg diet) and one dl-methionine group (6.4 g/kg diet) for 8 weeks. At the end of feeding trial, 15 fish from each treatment were challenged with Aeromonas hydrophila for 14 days. The results indicated that optimal MHA enhanced the capacity of fish against enteritis emergence, which might be related to the positive effects of MHA on intestinal immunological and physical barrier function in fish...
March 6, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28266180/serum-from-plasma-rich-in-growth-factors-regenerates-rabbit-corneas-by-promoting-cell-proliferation-migration-differentiation-adhesion-and-limbal-stemness
#10
Jaime Etxebarria, Sara Sanz-Lázaro, Raquel Hernáez-Moya, Vanesa Freire, Juan A Durán, María-Celia Morales, Noelia Andollo
PURPOSE: To evaluate the regenerating potential and the mechanisms through which the autologous serum derived from plasma rich in growth factors (s-PRGF) favours corneal wound healing in vitro and in vivo. METHODS: We compared the effect of various concentrations of s-PRGF versus fetal bovine serum (FBS) and control treatment in rabbit primary corneal epithelial and stromal cells and wounded rabbit corneas. Cell proliferation was measured using an enzymatic colorimetric assay...
March 7, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28259952/ppar%C3%A2-%C3%AE-agonist-rosiglitazone-protects-rat-peritoneal-mesothelial-cells-against-peritoneal-dialysis-solution%C3%A2-induced-damage
#11
Yun-Fang Zhang, Qi Wang, Yan-Yan Su, Jie-Lin Wang, Bao-Jun Hua, Shen Yang, Jun-Xia Feng, Hong-Yan Li
Long-term peritoneal dialysis (PD) leads to ultrafiltration failure (UFF). Peritoneal mesothelial cells, which form the innermost monolayer of the peritoneal cavity, have been shown to regulate various responses, including inflammation, in UFF. The present study was designed to investigate the effect of the peroxisome proliferator‑activated receptor‑γ (PPAR‑γ) agonist, rosiglitazone, on peritoneal dialysis solution (PDS)‑induced injuries in rat peritoneal mesothelial cells (RPMCs). RPMCs were cultured for different durations and with different concentrations of PDS...
February 13, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28259408/variants-of-%C3%AE-casofensin-a-bioactive-milk-peptide-differently-modulate-the-intestinal-barrier-in-vivo-and-ex-vivo-studies-in-rats
#12
Jérémie Bruno, Aurélie Nicolas, Sandra Pesenti, Jessica Schwarz, Jean-Luc Simon, Joëlle Léonil, Pascale Plaisancié
β-Casofensin is a bioactive milk peptide that modulates the intestinal barrier, particularly through its action on goblet cells. β-Casofensin corresponds to fragment (f) 94-123 of the bovine β-casein (β-CN) A2 variant. Fifteen genetic variants of bovine β-CN (A1-3, B-G, H1-2, I-L) are known, of which the A2, A1, and B forms are the most common. These variants differ from each other by the substitution of one or more AA, some of which are localized in f94 to 123. The aim of our study was to compare the intestinal effects of β-casofensin A2 and its 3 main variants: A1, A3, and B...
March 2, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28259136/therapeutic-effects-of-different-doses-of-polyethylene-glycosylated-porcine-glucagon-like-peptide-2-on-ulcerative-colitis-in-male-rats
#13
Ke-Ke Qi, Jia-Jia Lv, Jie Wu, Zi-Wei Xu
BACKGROUND: Polyethylene glycosylated (PEGylated) porcine glucagon-like peptide-2 (pGLP-2) considerably increases half-life and stability compared with the native pGLP-2, but the effective dose for intestinal damage is still unclear. This study aims to evaluate the available dose of polyethylene glycosylated porcine glucagon-like peptide-2 (PEG-pGLP-2), a modified, long-acting form of pGLP-2 in an experimental rat model of ulcerative colitis. METHODS: Thirty-five male rats were randomly assigned into five groups: control, dextran sodium sulphate (DSS), DSS + PEG-pGLP-2(L), DSS + PEG-pGLP-2(M) and DSS + PEG-pGLP-2(H)...
March 4, 2017: BMC Gastroenterology
https://www.readbyqxmd.com/read/28237245/the-formation-of-quiescent-glomerular-endothelial-cell-monolayer-in-vitro-is-strongly-dependent-on-the-choice-of-extracellular-matrix-coating
#14
Kamilla Pajęcka, Malik Nygaard Nielsen, Troels Krarup Hansen, Julie M Williams
BACKGROUND AND AIMS: Nephropathy involves pathophysiological changes to the glomerulus. The primary glomerular endothelial cells (GEnCs) have emerged as an important tool for studying glomerulosclerotic mechanisms and in the screening process for drug-candidates. The success of the studies is dependent on the quality of the cell model. Therefore, we set out to establish an easy, reproducible model of the quiescent endothelial monolayer with the use of commercially available extracellular matrices (ECMs)...
February 22, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28211084/endothelial-cells-biophysical-biochemical-and-chromosomal-aberrancies-in-high-glucose-condition-within-the-diabetic-range
#15
Aysa Rezabakhsh, Elahe Nabat, Mina Yousefi, Soheila Montazersaheb, Omid Cheraghi, Amir Mehdizadeh, Farzaneh Fathi, Ali Akbar Movassaghpour, Nasrin Maleki-Dizaji, Reza Rahbarghazi, Alireza Garjani
To date, many studies have been conducted to find out the underlying mechanisms of hyperglycemia-induced complications in diabetes mellitus, attributed to the cellular pathologies of different cells-especially endothelial cells. However, there are still many ambiguities and unresolved issues to be clarified. Here, we investigated the alteration in biophysical and biochemical properties in human umbilical vein endothelial cells exposed to a high-glucose concentration (30mM), comparable to glucose content in type 2 diabetes mellitus, over a course of 120 hours...
March 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28193579/zinc-enhances-intestinal-epithelial-barrier-function-through-the-pi3k-akt-mtor-signaling-pathway-in-caco-2-cells
#16
Yuxin Shao, Patricia G Wolf, Shuangshuang Guo, Yuming Guo, H Rex Gaskins, Bingkun Zhang
Zinc plays an important role in maintaining intestinal barrier function as well as modulating cellular signaling recognition and protein kinase activities. The phosphatidylinositol 3-kinase (PI3K) cascade has been demonstrated to affect intercellular integrity and tight junction (TJ) proteins. The current study investigated the hypothesis that zinc regulates intestinal intercellular junction integrity through the PI3K/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway. A transwell model of Caco-2 cell was incubated with 0, 50 and 100 μM of zinc at various time points...
January 31, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28193461/gill-structural-integrity-changes-in-fish-deficient-or-excessive-in-dietary-isoleucine-towards-the-modulation-of-tight-junction-protein-inflammation-apoptosis-and-antioxidant-defense-via-nf-%C3%AE%C2%BAb-tor-and-nrf2-signaling-pathways
#17
Lin Feng, Lu Gan, Wei-Dan Jiang, Pei Wu, Yang Liu, Jun Jiang, Ling Tang, Sheng-Yao Kuang, Wu-Neng Tang, Yong-An Zhang, Xiao-Qiu Zhou
This study firstly aimed to test the impact of dietary isoleucine (Ile) on tight junction protein, inflammation, apoptosis, antioxidant defense and related signaling molecule gene expression in the gill of fish. Young grass carp (Ctenopharyngodon idella) (weighing 256.8 ± 3.5 g) were fed six diets containing graded levels of Ile, namely, 3.8, 6.6, 9.3, 12.5, 15.2 and 18.5 g/kg diet for 8 weeks. The results firstly revealed that Ile deficiency down-regulated the mRNA expressions of claudin-3, claudin-b, claudin-c, occludin and zonula occludens-1 (ZO-1) and up-regulated the mRNA expression of claudin-12, which led to the intercellular structure damage of fish gill...
February 11, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28182318/molecular-and-structural-changes-related-to-hepatitis-e-virus-hev-antigen-and-its-expression-in-testis-inducing-apoptosis-in-mongolian-gerbil-model
#18
Majid Hussain Soomro, Ruihan Shi, Ruiping She, Yifei Yang, Tongtong Wang, Qiaoxing Wu, Heng Li, Wenzhuo Hao
Hepatitis E virus (HEV) infection has been associated with a wide range of extrahepatic manifestations, so this study was designed to examine the effect and role of HEV on structural and molecular changes in the testicular tissues of Mongolian gerbils experimentally infected with swine HEV. HEV RNA was first detected in testis at 14 day post inoculation and reached a peak between 28 to 42 days later with viral load between 3.12 logs g-¹ to 6.23 logs g-¹ by PCR assays. Microscopic changes showed vacuolation, sloughing of germ cells, formation of multinuclear giant cells, degeneration, necrosis of tubules and damaged blood-testis barrier was observed through transmission electron microscopy...
February 9, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28170537/adenosine-deaminase-2-induced-hyperpermeability-in-human-retinal-vascular-endothelial-cells-is-suppressed-by-microrna-146b-3p
#19
Yara A Samra, Heba M Saleh, Khaled A Hussein, Nehal M Elsherbiny, Ahmed S Ibrahim, Khaled Elmasry, Sadanand Fulzele, Mamdouh M El-Shishtawy, Laila A Eissa, Mohamed Al-Shabrawey, Gregory I Liou
Purpose: We recently demonstrated that adenosine deaminase-2 (ADA2) contributes to diabetic retinopathy (DR) via up-regulating the production of inflammatory cytokines in macrophages. Also, microRNA (miR)-146b-3p has the ability to inhibit ADA2. The goal of this study was to investigate the potential role of ADA2 and therapeutic benefit of miR-146b-3p in retinal inflammation and endothelial barrier dysfunction during diabetes. Methods: Adenosine deaminase-2 activity was determined by colorimetric method in diabetic human vitreous...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28156053/urothelial-cell-expansion-and-differentiation-are-improved-by-exposure-to-hypoxia
#20
Stéphane Chabaud, Ingrid Saba, Clément Baratange, Brice Boiroux, Maude Leclerc, Alexandre Rousseau, Sara Bouhout, Stéphane Bolduc
Cells obtained from a patient's biopsy have to be expanded after extraction to produce autologous tissues, but standard cell culture conditions often limit their growth or lifespan and could induce early and inadequate cell differentiation. Moreover, it has previously been reported that the air-liquid interface, that induces maturation of the urothelium, stimulated inadequate differentiation associated with aberrant keratin-14 expression. The aim of this study was to test the benefits of hypoxia during expansion of urothelial cells and maturation of the bladder epithelium in the context of tissue engineering...
February 3, 2017: Journal of Tissue Engineering and Regenerative Medicine
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