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https://www.readbyqxmd.com/read/28428961/glucose-insulin-potassium-alleviates-intestinal-mucosal-barrier-injuries-involving-decreased-expression-of-uncoupling-protein-2-and-nlr-family-pyrin-domain-containing-3-inflammasome-in-polymicrobial-sepsis
#1
Jun-Liang Zhang, Yi-Ting Chen, Guang-Dao Chen, Tao Wang, Ju-Xin Zhang, Qi-Yi Zeng
Uncoupling protein 2 (UCP2) may be critical for intestinal barrier function which may play a key role in the development of sepsis, and insulin has been reported to have anti-inflammatory effects. Male Sprague-Dawley rats were randomly allocated into five groups: control group, cecal ligation and puncture (CLP) group, sham surgery group, CLP plus glucose-insulin-potassium (GIK) group, and CLP plus glucose and potassium (GK) group. Ileum tissues were collected at 24 h after surgery. Histological and cytokine analyses, intestinal permeability tests, and western blots of intestinal epithelial tight junction component proteins and UCP2 were performed...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28428082/calcitonin-receptor-increases-invasion-of-prostate-cancer-cells-by-recruiting-zonula-occludens-1-and-promoting-pka-mediated-tj-disassembly
#2
Ahmed Aljamilee, Arvind Thakkar, Girish Shah
Almost all primary prostate cancers (PCs) and PC cell lines express calcitonin (CT) and/or its receptor (CTR), and their co-expression positively correlates with their invasiveness. Activation of the CT-CTR axis in non-invasive LNCaP cells induces an invasive phenotype. In contrast, silencing of CT/CTR expression in highly metastatic PC-3M cells markedly reduces their tumorigenicity and abolishes their ability to form distant metastases in nude mice. Our recent studies suggest that CTR interacts with zonula occludens 1 (ZO-1) through PDZ interaction to destabilize tight junctions and increase invasion of PC cells...
April 17, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28425012/a-study-of-the-damage-of-the-intestinal-mucosa-barrier-structure-and-function-of-ctenopharyngodon-idella-with-aeromonas-hydrophila
#3
Wei-Guang Kong, Si-Si Li, Xiao-Xuan Chen, Yu-Qing Huang, Ying Tang, Zhi-Xin Wu
The aim of this study is to explore the effect of Aeromonas hydrophila on the intestinal mucosal barrier structure and intestinal permeability in grass carp (Ctenopharyngodon idella). Histopathological examinations showed that A. hydrophila induced severe intestinal lesions, including inflammatory cell infiltration and intestinal villus fusion and swelling. Messenger RNA (mRNA) expression of the inflammatory cytokines TNF-α, IL-1β, IL-8, IL-10 and MyD88 was significantly increased after infection with A. hydrophila...
April 19, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28421532/matrix-metalloproteinase-8-inhibition-prevents-disruption-of-blood-spinal-cord-barrier-and-attenuates-inflammation-in-rat-model-of-spinal-cord-injury
#4
Hemant Kumar, Min-Jae Jo, Hyemin Choi, Manjunatha S Muttigi, Seil Shon, Byung-Joo Kim, Soo-Hong Lee, In-Bo Han
After spinal cord injury (SCI), tight junction (TJ) protein degradation increases permeability and disrupts the blood-spinal cord barrier (BSCB). The BSCB is primarily formed of endothelial cell, which forms a specialized tight seal due to the presence of TJs. BSCB disruption after SCI allows neutrophil infiltration. Matrix metalloproteinase (MMP)-8 is believed to be mainly expressed by neutrophils and is quickly released upon neutrophil activation. Here, we determined whether MMP-8 is involved in the TJ protein degradation in endothelial cells and also determined its role in the neuroinflammation after SCI...
April 18, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28405145/acanthopanax-senticosus-polysaccharides-induced-intestinal-tight-junction-injury-alleviation-via-inhibition-of-nf-%C3%AE%C2%BAb-mlck-pathway-in-a-mouse-endotoxemia-model
#5
Jie Han, Ji-Hong Li, Guang Bai, Guo-Shun Shen, Jing Chen, Jia-Nan Liu, Shuo Wang, Xian-Jun Liu
AIM: To examine the effects of Acanthopanax senticosus polysaccharides (ASPS) on intestinal tight junction (TJ) disruption and nuclear factor-kappa B (NF-κB)/myosin light chain kinase (MLCK) activation in endotoxemia. METHODS: BALB/C mice (6-8-weeks-old) received continuous intragastric gavage of ASPS for 7 d before injection of lipopolysaccharide (LPS), or received ASPS once after LPS injection. Blood and intestinal mucosal samples were collected 6 h after LPS challenge...
March 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28378130/the-role-of-actin-depolymerizing-factor-in-advanced-glycation-endproducts-induced-impairment-in-mouse-brain-microvascular-endothelial-cells
#6
Xinfeng Liu, Rongju Zhang, Hai Di, Dengfa Zhao, Jun Wang
Endothelial dysfunction is one of the most important pathological events during the development of several diabetic complications including stroke. The aim of this work was to investigate the role of actin depolymerizing factor (ADF) in advanced glycation endproducts (AGEs)-induced impairment in mouse brain microvascular endothelial cells (MBMECs). Incubation of MBMECs with AGEs led to downregulation of expression of ADF, which was reversed by treatment with soluble receptor of AGEs or tempol (an antioxidant)...
April 4, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28370715/l-fermentum-cect-5716-prevents-stress-induced-intestinal-barrier-dysfunction-in-newborn-rats
#7
T Vanhaecke, P Aubert, P-A Grohard, T Durand, P Hulin, P Paul-Gilloteaux, A Fournier, F Docagne, A Ligneul, C Fressange-Mazda, P Naveilhan, H Boudin, P Le Ruyet, M Neunlist
BACKGROUND: Intestinal epithelial barrier (IEB) dysfunction plays a critical role in various intestinal disorders affecting infants and children, including the development of food allergies and colitis. Recent studies highlighted the role of probiotics in regulating IEB functions and behavior in adults, but their effects in the newborn remain largely unknown. We therefore characterized in rat pups, the impact of Lactobacillus fermentum CECT 5716 (L. fermentum) on stress-induced IEB dysfunction, systemic immune response and exploratory behavior...
March 31, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28368613/in-vivo-study-of-the-efficacy-of-the-essential-oil-of-zanthoxylum-bungeanum-pericarp-in-dextran-sulfate-sodium-induced-murine-experimental-colitis
#8
Zecai Zhang, Peng Shen, Jiuxi Liu, Cong Gu, Xiaojie Lu, Yanxin Li, Yongguo Cao, Bo Liu, Yunhe Fu, Naisheng Zhang
The purpose of this study was to investigate the protective effects and mechanisms of the essential oil of Zanthoxylum bungeanum pericarp (ZBEO) on dextran sulfate sodium (DSS)-induced experimental colitis in mice. ZBEO decreased DSS-induced body weight loss, the disease activity index, colon length shortening, colonic pathological damage, and myeloperoxidase activities. The production of pro-inflammatory mediators was significantly alleviated by ZBEO. Further mechanistic analysis showed that ZBEO inhibited inflammation by regulating NF-κB and PPARγ pathways...
April 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28339048/agr2-ameliorates-tumor-necrosis-factor-%C3%AE-induced-epithelial-barrier-dysfunction-via-suppression-of-nf-%C3%AE%C2%BAb-p65-mediated-mlck-p-mlc-pathway-activation
#9
Xiaolin Ye, Mei Sun
Intestinal epithelial barrier dysfunction plays a critical role in the pathogenesis of inflammatory bowel disease (IBD). Anterior gradient protein 2 homologue (AGR2) assists in maintaining intestinal homeostasis in dextran sulphate sodium-induced mouse ileocolitis; however, it is unclear whether it modulates intestinal barrier function. Our study aimed to investigate the protective role of AGR2 in tumor necrosis factor (TNF)-α-induced intestinal epithelial barrier injury. Caco-2 cell monolayers were pre-transfected with an AGR2 plasmid and then exposed to TNF-α...
March 21, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28333312/establishment-maintenance-and-functional-integrity-of-the-blood-testis-barrier-in-organotypic-cultures-of-fresh-and-frozen-thawed-prepubertal-mouse-testes
#10
C Rondanino, A Maouche, L Dumont, A Oblette, N Rives
STUDY QUESTION: Can the spatio-temporal formation of an intact blood-testis barrier (BTB), which is essential for the progression of spermatogenesis, be reproduced in cultures of fresh or frozen/thawed prepubertal mouse testes? SUMMARY ANSWER: Organotypic cultures allow the establishment and maintenance of major BTB components and the formation of a functional BTB in mouse testicular tissues. WHAT IS KNOWN ALREADY: In vitro maturation of prepubertal testicular tissues is a promising approach to restore fertility in adult survivors of childhood cancer...
March 17, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28328069/autophagy-maintains-the-integrity-of-endothelial-barrier-in-lps-induced-lung-injury
#11
Dan Zhang, Jian Zhou, Le Chi Ye, Jing Li, Zhenzhou Wu, Yuping Li, Chichi Li
Understanding the role and underlying regulation mechanism of autophagy in lipopolysaccharide-induced lung injury (LPS-LI) may provide potentially new pharmacological targets for treatment of acute lung injury. The aim of this study was to investigate the functional significance of autophagy in LPS-LI. The autophagy of human pulmonary microvascular endothelial cells (HPMVECs) and mice was inhibited before they were challenged with LPS. In vitro, permeability, vitality and the lactate dehydrogenase (LDH) release rate of the cells were detected, the zonula occluden-1 (ZO-1) expression and the stress fiber formation were determined...
March 22, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28323272/constipation-predominant-irritable-bowel-syndrome-females-have-normal-colonic-barrier-and-secretory-function
#12
Stephanie A Peters, Shoko Edogawa, Wendy J Sundt, Roy B Dyer, Daniel A Dalenberg, Amelia Mazzone, Ravinder J Singh, Natalie Moses, Thomas C Smyrk, Christopher Weber, David R Linden, Wallace K MacNaughton, Jerrold R Turner, Michael Camilleri, David A Katzka, Gianrico Farrugia, Madhusudan Grover
OBJECTIVES: The objective of this study was to determine whether constipation-predominant irritable bowel syndrome (IBS-C) is associated with changes in intestinal barrier and secretory function. METHODS: A total of 19 IBS-C patients and 18 healthy volunteers (all females) underwent saccharide excretion assay (0.1 g (13)C mannitol and 1 g lactulose), measurements of duodenal and colonic mucosal barrier (transmucosal resistance (TMR), macromolecular and Escherichia coli Bio-Particle translocation), mucosal secretion (basal and acetylcholine (Ach)-evoked short-circuit current (Isc)), in vivo duodenal mucosal impedance, circulating endotoxins, and colonic tight junction gene expression...
March 21, 2017: American Journal of Gastroenterology
https://www.readbyqxmd.com/read/28320523/roles-of-fluid-shear-stress-and-retinoic-acid-in-the-differentiation-of-primary-cultured-human-podocytes
#13
Seung Hee Yang, Jin Woo Choi, Dongeun Huh, Hyung Ah Jo, Sejoong Kim, Chun Soo Lim, Jung Chan Lee, Hee Chan Kim, Hyug Moo Kwon, Chang Wook Jeong, Cheol Kwak, Kwon Wook Joo, Yon Su Kim, Dong Ki Kim
Due to the distinct features that distinguish immortalized podocyte cell lines from their in vivo counterparts, primary cultured human podocytes might be a superior cell model for glomerular disease studies. However, the podocyte de-differentiation that occurs in culture remains an unresolved problem. Here, we present a method to differentiate primary cultured podocytes using retinoic acid (RA) and fluid shear stress (FSS), which mimic the in vivo environment of the glomerulus. RA treatment induced changes in the cell shape of podocytes from a cobblestone-like morphology to an arborized configuration with enhanced mobility...
March 16, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28303918/effects-of-matrix-stiffness-on-epithelial-to-mesenchymal-transition-like-processes-of-endometrial-epithelial-cells-implications-for-the-pathogenesis-of-endometriosis
#14
Sachiko Matsuzaki, Claude Darcha, Jean-Luc Pouly, Michel Canis
Endometriosis is defined as the presence of endometrial glands and stroma within extrauterine sites. Our previous study revealed an epithelial to mesenchymal transition (EMT)-like process in red peritoneal endometriosis, whereas membrane localization of E-cadherin was well maintained in epithelial cells of deep infiltrating endometriosis (DIE). Here we show that endometrial epithelial cells (EEE) grown on polyacrylamide gel substrates (PGS) of 2 kilopascal (kPa), a soft matrix, initiate a partial EMT-like process with transforming growth factor-β1 (TGF-β1) stimulation...
March 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28300333/psychological-stress-induced-colonic-barrier-dysfunction-role-of-immune-mediated-mechanisms
#15
P Hattay, D K Prusator, L Tran, B Greenwood-Van Meerveld
BACKGROUND: Evidence suggests that patients with irritable bowel syndrome (IBS) exhibit increases in gut permeability and alterations in tight junction (TJ) protein expression. Although psychological stress worsens IBS symptoms, the mechanisms by which stress enhances gut permeability and affects TJ protein expression remain to be determined. Here, we test the hypothesis that chronic intermittent psychological stress activates the release of proinflammatory cytokines to alter TJ proteins and promotes increased gut permeability...
March 16, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28287613/knockdown-of-long-non-coding-rna-xist-increases-blood-tumor-barrier-permeability-and-inhibits-glioma-angiogenesis-by-targeting-mir-137
#16
H Yu, Y Xue, P Wang, X Liu, J Ma, J Zheng, Z Li, Z Li, H Cai, Y Liu
Antiangiogenic therapy plays a significant role in combined glioma treatment. However, poor permeability of the blood-tumor barrier (BTB) limits the transport of chemotherapeutic agents, including antiangiogenic drugs, into tumor tissues. Long non-coding RNAs (lncRNAs) have been implicated in various diseases, especially malignant tumors. The present study found that lncRNA X-inactive-specific transcript (XIST) was upregulated in endothelial cells that were obtained in a BTB model in vitro. XIST knockdown increased BTB permeability and inhibited glioma angiogenesis...
March 13, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28279791/methionine-hydroxy-analogue-improves-intestinal-immunological-and-physical-barrier-function-in-young-grass-carp-ctenopharyngodon-idella
#17
Fei-Yu Pan, Pei Wu, Lin Feng, Wei-Dan Jiang, Sheng-Yao Kuang, Ling Tang, Wu-Neng Tang, Yong-An Zhang, Xiao-Qiu Zhou, Yang Liu
This study was conducted to test the hypothesis that methionine hydroxy analogue (MHA) enhances the defense against enteritis occurrence via improving intestinal barrier function in fish. After 630 young grass carp (Ctenopharyngodon idella) (259.70 ± 0.47 g) fed six graded levels of MHA (0, 2.4, 4.4, 6.4, 8.5 and 10.5 g/kg diet) and one dl-methionine group (6.4 g/kg diet) for 8 weeks. At the end of feeding trial, 15 fish from each treatment were challenged with Aeromonas hydrophila for 14 days. The results indicated that optimal MHA enhanced the capacity of fish against enteritis emergence, which might be related to the positive effects of MHA on intestinal immunological and physical barrier function in fish...
May 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28266180/serum-from-plasma-rich-in-growth-factors-regenerates-rabbit-corneas-by-promoting-cell-proliferation-migration-differentiation-adhesion-and-limbal-stemness
#18
Jaime Etxebarria, Sara Sanz-Lázaro, Raquel Hernáez-Moya, Vanesa Freire, Juan A Durán, María-Celia Morales, Noelia Andollo
PURPOSE: To evaluate the regenerating potential and the mechanisms through which the autologous serum derived from plasma rich in growth factors (s-PRGF) favours corneal wound healing in vitro and in vivo. METHODS: We compared the effect of various concentrations of s-PRGF versus fetal bovine serum (FBS) and control treatment in rabbit primary corneal epithelial and stromal cells and wounded rabbit corneas. Cell proliferation was measured using an enzymatic colorimetric assay...
March 7, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28259952/ppar%C3%A2-%C3%AE-agonist-rosiglitazone-protects-rat-peritoneal-mesothelial-cells-against-peritoneal-dialysis-solution%C3%A2-induced-damage
#19
Yun-Fang Zhang, Qi Wang, Yan-Yan Su, Jie-Lin Wang, Bao-Jun Hua, Shen Yang, Jun-Xia Feng, Hong-Yan Li
Long-term peritoneal dialysis (PD) leads to ultrafiltration failure (UFF). Peritoneal mesothelial cells, which form the innermost monolayer of the peritoneal cavity, have been shown to regulate various responses, including inflammation, in UFF. The present study was designed to investigate the effect of the peroxisome proliferator‑activated receptor‑γ (PPAR‑γ) agonist, rosiglitazone, on peritoneal dialysis solution (PDS)‑induced injuries in rat peritoneal mesothelial cells (RPMCs). RPMCs were cultured for different durations and with different concentrations of PDS...
April 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28259408/variants-of-%C3%AE-casofensin-a-bioactive-milk-peptide-differently-modulate-the-intestinal-barrier-in-vivo-and-ex-vivo-studies-in-rats
#20
Jérémie Bruno, Aurélie Nicolas, Sandra Pesenti, Jessica Schwarz, Jean-Luc Simon, Joëlle Léonil, Pascale Plaisancié
β-Casofensin is a bioactive milk peptide that modulates the intestinal barrier, particularly through its action on goblet cells. β-Casofensin corresponds to fragment (f) 94-123 of the bovine β-casein (β-CN) A2 variant. Fifteen genetic variants of bovine β-CN (A1-3, B-G, H1-2, I-L) are known, of which the A2, A1, and B forms are the most common. These variants differ from each other by the substitution of one or more amino acids, some of which are localized in f94 to 123. The aim of our study was to compare the intestinal effects of β-casofensin A2 and its 3 main variants: A1, A3, and B...
May 2017: Journal of Dairy Science
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