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Bacillus thuringiensis receptors

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https://www.readbyqxmd.com/read/29777666/analysis-of-cross-resistance-to-vip3-proteins-in-eight-insect-colonies-from-four-insect-species-selected-for-resistance-to-bacillus-thuringiensis-insecticidal-proteins
#1
Joaquín Gomis-Cebolla, Yuequin Wang, Yudong Quan, Kanglai He, Tom Walsh, Bill James, Sharon Downes, Wendy Kain, Ping Wang, Kathy Leonard, Tom Morgan, Brenda Oppert, Juan Ferré
Bacillus thuringiensis Vip3 proteins are synthesized and secreted during the vegetative growth phase. They are activated by gut proteases, recognize and bind to midgut receptors, form pores and lyse cells. We tested the susceptibility to Vip3Aa and Vip3Ca of Cry1A-, Cry2A-, Dipel- and Vip3-resistant insect colonies from different species to determine whether resistance to other insecticidal proteins confers cross-resistance to Vip3 proteins. As expected, the colonies resistant to Cry1A proteins, Dipel (Helicoverpa armigera, Trichoplusia ni, Ostrinia furnacalis and Plodia interpunctella) or Cry2Ab (H...
May 16, 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29775454/in-vivo-and-in-vitro-studies-of-cry5b-and-nicotinic-acetylcholine-receptor-agonist-anthelmintics-reveal-a-powerful-and-unique-combination-therapy-against-intestinal-nematode-parasites
#2
Yan Hu, Melanie Miller, Bo Zhang, Thanh-Thanh Nguyen, Martin K Nielsen, Raffi V Aroian
BACKGROUND: The soil-transmitted nematodes (STNs) or helminths (hookworms, whipworms, large roundworms) infect the intestines of ~1.5 billion of the poorest peoples and are leading causes of morbidity worldwide. Only one class of anthelmintic or anti-nematode drugs, the benzimidazoles, is currently used in mass drug administrations, which is a dangerous situation. New anti-nematode drugs are urgently needed. Bacillus thuringiensis crystal protein Cry5B is a powerful, promising new candidate...
May 18, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29767964/a-synthetic-polymer-affinity-ligand-for-bacillus-thuringiensis-bt-cry1ab-ac-protein-the-use-of-biomimicry-based-on-the-bt-protein-insect-receptor-binding-mechanism
#3
Mingming Liu, Rong Huang, Adam C Weisman, Xiaoyang Yu, Shih-Hui Lee, Yalu Chen, Chao Huang, Senhua Hu, Xiuhua Chen, Wenfeng Tan, Fan Liu, Hao Chen, Kenneth J Shea
We report a novel strategy for creating abiotic Bacillus thuringiensis (Bt) protein affinity ligands by biomimicry of the recognition process that takes place between Bt Cry1Ab/Ac proteins and insect receptor cadherin-like Bt-R1 proteins. Guided by this strategy, a library of synthetic polymer nanoparticles (NPs) was prepared and screened for binding to three epitopes 280FRGSAQGIEGS290, 368RRPFNIGINNQQ379 and 436FRSGFSNSSVSIIR449 located in loop α8, loop 2 and loop 3 of domain II of Bt Cry1Ab/Ac proteins. A negatively charged and hydrophilic nanoparticle (NP12) was found to have high affinity to one of the epitopes, 368RRPFNIGINNQQ379...
May 16, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29700928/labeling-membrane-receptors-with-lectins-and-evaluate-on-of-the-midgut-histochemistry-of-tuta-absoluta-meyrick-lepidoptera-gelechiidae-populations-with-different-levels-of-susceptibility-to-formulated-bt
#4
Andresa C B Oliveira, Valeria Wanderley-Teixeira, Cristiane T S Silva, Álvaro A C Teixeira, Herbert A A Siqueira, Glauciane S Cruz, Clovis L Neto, Amanda L R Lima, Maria T S Correia
BACKGROUND: Studies show that insects can adapt to the toxins of Bacillus thuringiensis in field and laboratory conditions through the development of resistance to this bacterium and its formulations. This has been demonstrated in the failure to control Tuta absoluta populations in Brazil. This study evaluated membrane receptors using peroxidase labelled lectins and the midgut histochemistry of T. absoluta populations in order to assess susceptibility to the insecticides Dipel® and XenTari® ...
April 26, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29686508/first-report-of-detection-of-the-putative-receptor-of-bacillus-thuringiensis-toxin-vip3aa-from-black-cutworm-agrotis-ipsilon
#5
Gamal H Osman, Waleed J Altaf, Ibrahim A S Saleh, Raya Soltane, Hussein H Abulreesh, Ibrahim A Arif, Ahmed M Ramadan, Yehia A Osman
Black cutworm (BCW) Agrotis ipsilon , an economically important lepidopteran insect, has attracted a great attention. Bacillus thuringiensis (Bt) is spore forming soil bacteria and is an excellent environment-friendly approach for the control of phytophagous and disease-transmitting insects. In fact, bio-pesticide formulations and insect resistant transgenic plants based on the bacterium Bt delta-endotoxin have attracted worldwide attention as a safer alternative to harmful chemical pesticides. The major objective of the current study was to understand the mechanism of interaction of Bt toxin with its receptor molecule(s)...
March 2018: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/29666188/extracellular-loop-structures-in-silkworm-abcc-transporters-determine-their-specificities-for-bacillus-thuringiensis-cry-toxins
#6
Haruka Endo, Shiho Tanaka, Satomi Adegawa, Fumika Ichino, Hiroko Tabunoki, Shingo Kikuta, Ryoichi Sato
Bacillus thuringiensis Cry toxins are insecticidal proteins used widely for pest control. They are lethal to a restricted range of insects via specific interactions with insect receptors such as the ATP-binding cassette transporter subfamily members C2 (ABCC2) and C3 (ABCC3). It is unclear how these different receptors contribute to insect susceptibility to Cry1A toxins. Here, we investigated the differences between the silkworm (Bombyx mori) ABCC2 (BmABCC2_S) and ABCC3 (BmABCC3) receptors in mediating Cry toxicity...
April 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29563565/engineering-bacillus-thuringiensis-cyt1aa-toxin-specificity-from-dipteran-to-lepidopteran-toxicity
#7
Mary-Carmen Torres-Quintero, Isabel Gómez, Sabino Pacheco, Jorge Sánchez, Humberto Flores, Joel Osuna, Gretel Mendoza, Mario Soberón, Alejandra Bravo
The Cyt and Cry toxins are different pore-forming proteins produced by Bacillus thuringiensis bacteria, and used in insect-pests control. Cry-toxins have a complex mechanism involving interaction with several proteins in the insect gut such as aminopeptidase N (APN), alkaline phosphatase (ALP) and cadherin (CAD). It was shown that the loop regions of domain II of Cry toxins participate in receptor binding. Cyt-toxins are dipteran specific and interact with membrane lipids. We show that Cry1Ab domain II loop3 is involved in binding to APN, ALP and CAD receptors since point mutation Cry1Ab-G439D affected binding to these proteins...
March 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29547530/the-replacement-of-five-consecutive-amino-acids-in-the-cyt1a-protein-of-bacillus-thuringiensis-enhances-its-cytotoxic-activity-against-lung-epithelial-cancer-cells
#8
Kavita Nair, Ahmad Iskandarani, Roda Al-Thani, Ramzi Mohammad, Samir Jaoua
Cyt1A protein is a cytolytic protein encoded by the cyt gene of Bacillus thuringiensis subsp. israelensis (Bti) as part of the parasporal crystal proteins produced during the sporulation. Cyt1A protein is unique compared to the other endotoxins present in these parasporal crystals. Unlike δ-endotoxins, Cyt1A protein does not require receptors to bind to the target cell and activate the toxicity. It has the ability to affect a broad range of cell types and organisms, due to this characteristic. Cyt1A has been recognized to not only target the insect cells directly, but also recruit other endotoxins by acting as receptors...
March 16, 2018: Toxins
https://www.readbyqxmd.com/read/29546335/alimentary-tract-transcriptome-analysis-of-the-tea-geometrid-ectropis-oblique-lepidoptera-geometridae
#9
Junxiang Wang, Guifang Lin, Khadija Batool, Shuaiqi Zhang, Mingfeng Chen, Jin Xu, Juan Wu, Liang Jin, Ivan Gelbic, Lei Xu, Lingling Zhang, Xiong Guan
Ectropis oblique Prout (Lepidoptera: Geometridae) is one of the main pests that damages the tea crop in Southeast Asia. To understand the molecular mechanisms of its feeding biology, transcriptomes of the alimentary tract (AT) and of the body minus the AT of E. oblique were successfully sequenced and analyzed in this study. A total of 36,950 unigenes from de novo sequences were assembled. After analysis using six annotation databases (e.g., Gene Ontology, Kyoto Encyclopedia of Genes and Genome, and NCBI nr), a series of putative genes were found for this insect species that were related to digestion, detoxification, the immune system, and Bacillus thuringiensis (Bt) receptors...
March 12, 2018: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29502179/nematode-specific-cadherin-cdh-8-acts-as-a-receptor-for-cry5b-toxin-in-caenorhabditis-elegans
#10
Donghai Peng, Danfeng Wan, Chunsheng Cheng, Xiaobo Ye, Ming Sun
Parasitic nematodes of animals and plants cause worldwide devastating impacts on people's lives and agricultural crops. The crystal protein Cry5B produced by Bacillus thuringiensis has efficient and specific activity against a wide range of nematodes. However, the action mode of this toxin has not yet been thoroughly determined. Here, a nematode-specific cadherin CDH-8 was demonstrated to be a receptor for Cry5B toxin by using Caenorhabditis elegans as a model, providing evidence that the cadherin mutant worm cdh-8(RB815) possesses significant resistance to Cry5B, and the CDH-8 fragments bind specifically to Cry5B...
April 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29448008/insecticidal-activity-putative-binding-proteins-and-histopathological-effects-of-bacillus-thuringiensis-vip3-459-toxin-on-the-lepidopteran-pest-ectomyelois-ceratoniae
#11
Hanen Boukedi, Slim Tounsi, Lobna Abdelkefi-Mesrati
The carob moth, Ectomyelois ceratoniae, is an important agricultural pest that is susceptible to the Vip3(459) protein. The insecticidal activity, evaluated against this lepidopteran pest, displayed an LC50 value of about 28 ng/cm2 . The investigation of the mode of action of this B. thuringiensis protein demonstrated that the active form of this toxin bound to putative receptors in the BBMV of E. ceratoniae. Ligand blotting experiment proved that Vip3(459) specifically bound to two proteins of about 53 and 57 kDa, located on the midgut...
June 2018: Acta Tropica
https://www.readbyqxmd.com/read/29425691/the-intracellular-region-of-silkworm-cadherin-like-protein-is-not-necessary-to-mediate-the-toxicity-of-bacillus-thuringiensis-cry1aa-and-cry1ab-toxins
#12
Haruka Endo, Satomi Adegawa, Shingo Kikuta, Ryoichi Sato
The cadherin-like protein in lepidopteran insects, known as a receptor for Bacillus thuringiensis Cry1A toxins, is a single-pass membrane protein that can be divided into extracellular and intracellular regions. The extracellular region is important for toxin binding and oligomerization, whereas the role of the intracellular region during Cry1A intoxication is unclear. In the present study, we generated a deletion mutant of Bombyx mori cadherin-like protein (BtR175) that lacked the intracellular region to investigate its role in mediating Cry1A toxicity...
March 2018: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29415259/analysis-of-homologs-of-cry-toxin-receptor-related-proteins-in-the-midgut-of-a-non-bt-target-nilaparvata-lugens-st%C3%A3-l-hemiptera-delphacidae
#13
Ensi Shao, Li Lin, Sijun Liu, Jiao Zhang, Xuelin Chen, Li Sha, Zhipeng Huang, Biwang Huang, Xiong Guan
The brown planthopper (BPH) Nilaparvata lugens is one of the most destructive insect pests in the rice fields of Asia. Like other hemipteran insects, BPH is not susceptible to Cry toxins of Bacillus thuringiensis (Bt) or transgenic rice carrying Bt cry genes. Lack of Cry receptors in the midgut is one of the main reasons that BPH is not susceptible to the Cry toxins. The main Cry-binding proteins (CBPs) of the susceptible insects are cadherin, aminopeptidase N (APN), and alkaline phosphatase (ALP). In this study, we analyzed and validated de novo assembled transcripts from transcriptome sequencing data of BPH to identify and characterize homologs of cadherin, APN, and ALP...
February 5, 2018: Journal of Insect Science
https://www.readbyqxmd.com/read/29408155/the-full-length-cry1ac-protoxin-without-proteolytic-activation-exhibits-toxicity-against-insect-cell-line-cf-203
#14
Xiaodi Li, Feng Zhao, Xianfeng Qiu, Xiaomeng Ren, Xiangtao Mo, Xuezhi Ding, Liqiu Xia, Yunjun Sun
The new dual model for Bacillus thuringiensis insecticidal mechanism proposed that Cry1A protoxins without proteolytic activation could bind to insect midgut receptors to exert toxicity. To evaluate insecticidal potency of Cry1Ac protoxin at precluding interference of midgut proteases, the cytotoxicity of Cry1Ac protoxin against midgut cell line CF-203 derived from Choristoneura fumiferana was analyzed. It was revealed that Cry1Ac protoxin was toxic to CF-203 cells and there existed certain differences in the cytological changes when treated with protoxin and toxin...
February 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29380507/exposure-of-helices-%C3%AE-4-and-%C3%AE-5-is-required-for-insecticidal-activity-of-cry2ab-by-promoting-assembly-of-a-prepore-oligomeric-structure
#15
Lian Xu, Zhi-Zhen Pan, Jing Zhang, Li-Yang Niu, Jie Li, Zheng Chen, Bo Liu, Yu-Jing Zhu, Qing-Xi Chen
Cry2Ab, a pore-forming toxin derived from Bacillus thuringiensis, is widely used as a bio-insecticide to control lepidopteran pests around the world. A previous study revealed that proteolytic activation of Cry2Ab by Plutella xylostella midgut juice was essential for its insecticidal activity against P. xylostella, although the exact molecular mechanism remained unknown. Here, we demonstrated for the first time that proteolysis of Cry2Ab uncovered an active region (the helices α4 and α5 in Domain I), which was required for the mode of action of Cry2Ab...
January 30, 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29378203/comparative-analysis-of-the-susceptibility-tolerance-of-spodoptera-littoralis-to-vip3aa-vip3ae-vip3ad-and-vip3af-toxins-of-bacillus-thuringiensis
#16
Hanen Boukedi, Saoussen Ben Khedher, Lobna Abdelkefi-Mesrati, Jeroen Van Rie, Slim Tounsi
The cotton leaf worm Spodoptera littoralis is known for causing serious damages to various crops. In this study, the susceptibility/tolerance of this larvae to four Vip3A (Vip3Aa, Vip3Ae, Vip3Ad and Vip3Af) toxins was investigated. UnlikeVip3Ad which showed no activity to S. littoralis, Vip3Aa, Vip3Ae and Vip3Af exhibited high toxicity to this larva with LC50 of 228.42 ng/cm2 , 65.71 ng/cm2 , and 388.90 ng/cm2 , respectively. Activation of the 90 kDa Vip3A proteins by S. littoralis larvae juice generated four major bands of sizes 62, 45, 33 and 22 kDa...
February 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29366893/molecular-characterization-of-cry1d-133-toxin-from-bacillus-thuringiensis-strain-hd133-and-its-toxicity-against-spodoptera-littoralis
#17
Dalel BenFarhat-Touzri, Fatma Driss, Sonia Jemli, Slim Tounsi
Bacillus thuringiensis subsp. aizawai strain HD133, known by its effectiveness against Spodoptera species, produces bipyramidal crystals encompassing the insecticidal proteins Cry1Ab, Cry1Ca and Cry1D-133 in the proportions 60:37:3, respectively. In this study, we dealt with the relevance of the low rate of Cry1D-133. The cry1D-133 gene from HD133 was cloned and sequenced. Both nucleotide and amino acid sequence similarity analyses with the cry1D genes available in the GenBank database revealed that cry1D-133 is a new variant of cry1Da-type genes with 99% identity with cry1Da1...
June 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29305214/expression-of-cry1ac-toxin-binding-region-in-plutella-xyllostella-cadherin-like-receptor-and-studying-their-interaction-mode-by-molecular-docking-and-site-directed-mutagenesis
#18
Xiaodan Hu, Xiao Zhang, Jianfeng Zhong, Yuan Liu, Cunzheng Zhang, Yajing Xie, Manman Lin, Chongxin Xu, Lina Lu, Qing Zhu, Xianjin Liu
Cadherin-like protein has been identified as the primary Bacillus thuringiensis (Bt) Cry toxin receptor in Lepidoptera pests and plays a key role in Cry toxin insecticidal. In this study, we successfully expressed the putative Cry1Ac toxin-binding region (CR7-CR11) of Plutella xylostella cadherin-like in Escherichia coli BL21 (DE3). The expressed CR7-CR11 fragment showed binding ability to Cry1Ac toxin under denaturing (Ligand blot) and non-denaturing (ELISA) conditions. The three-dimensional structure of CR7-CR11 was constructed by homology modeling...
May 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29193387/the-nuclear-hormone-receptor-e75a-regulates-vitellogenin-gene-al-vg-expression-in-the-mirid-bug-apolygus-lucorum
#19
Y-A Tan, X-D Zhao, Y Sun, D-J Hao, J Zhao, Y-P Jiang, L-X Bai, L-B Xiao
Apolygus lucorum is the predominant pest of Bacillus thuringiensis (Bt) cotton in China. 20-hydroxyecdysone (20E) plays a key role in the reproduction of this insect. To better understand the mechanism underlying 20E-regulated reproduction, the nuclear hormone receptor E75 isoform-A of Ap. lucorum (Al-E75A) was cloned and its expression analysed. A 2241-bp sequence of Al-E75A cDNA encoded an open reading frame of a polypeptide with a predicted molecular mass of 69.04 kDa. Al-E75A mRNA was detected in female adult stages of Ap...
November 29, 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/29128667/bombyx-mori-abc-transporter-c2-structures-responsible-for-the-receptor-function-of-bacillus-thuringiensis-cry1aa-toxin
#20
Shiho Tanaka, Haruka Endo, Satomi Adegawa, Ami Iizuka, Kazuhiro Imamura, Shingo Kikuta, Ryoichi Sato
Because Bombyx mori ABC transporter C2 (BmABCC2) has 1000-fold higher potential than B. mori cadherin-like protein as a receptor for Bacillus thuringiensis Cry1Aa toxin (Tanaka et al., 2013), the gate-opening ability of the latent pore under six extracellular loops (ECLs) of BmABCC2 was expected to be the reason for its higher potential (Heckel, 2012). In this study, cell swelling assays in Sf9 cells showed that BmABCC2 mutants lacking substrate-excreting activity retained receptor activity, indicating that the gate-opening activity of BmABCC2 is not responsible for Cry1Aa toxicity...
December 2017: Insect Biochemistry and Molecular Biology
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