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Li-Chieh Lin, Osama Elkashty, Murali Ramamoorthi, Nathalie Trinh, Younan Liu, Gulshan Sunavala-Dossabhoy, Thomas Pranzatelli, Drew G Michael, Clara Chivasso, Jason Perret, John A Chiorini, Christine Delporte, Simon D Tran
The Human Salivary Gland cell line (HSG), labelled as a submandibular ductal cell line, is commonly used as in vitro models to study radiation therapy, Sjögren's syndrome, pleomorphic adenoma, mucocele, epithelial-to-mesenchymal transition, and epigenetics. However, the American Type Culture Collection (ATCC) has recently released a list of cross-contaminated cell lines that included HSG. Despite this notice, some research labs still use HSG as a salivary cell model. Accordingly, this study examined the authenticity of HSG sampled from three different laboratories METHODS: DNA was extracted from HSG and additional salivary cell lines (NS-SV-AC, NS-SV-DC, A253, HSY), and submitted for cell line authentication with short tandem repeat (STR) analysis RESULTS: All HSG samples had STR profiles indicating > 80% match with HeLa in both the ATCC and Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) databases...
June 20, 2018: Oral Diseases
Hannah Shoval, Esther Antelis, Andrew Hillman, Xiaofang Wei, Patricia Tan, Ruth Alejandro, Heakyung Kim
OBJECTIVE: To investigate the safety and efficacy of Onabotulinum Toxin-A (BTX-A) injection to the salivary glands under ultrasound guidance for the treatment of sialorrhea in patients with spinal muscular atrophy type I (SMA I). DESIGN: Prospective case series with four patients with SMA I who received BTX-A injection to parotid and submandibular glands for sialorrhea as part of clinical care. All four patients received validated surveys for measuring drooling, including objective measures of number of bib changes, and number of mouth wipes before injection and 4-6 weeks after injection...
June 18, 2018: American Journal of Physical Medicine & Rehabilitation
Shreya Sengupta, Sayantan Bose
We present a case of a 35-year-old gentleman with a submandibular duct stone measuring 12 × 6 mm. Considering the literature, most stones are less than 5 mm, and stones more than 10 mm are quite unusual. This gentleman had typical symptoms of chronic sialadenitis, who was clinically diagnosed to have sialolithiasis, which was later confirmed by imaging studies. He was operated upon to remove the stone along with the submandibular gland. The term sialolithiasis is derived from the Greek words sialon (saliva) and lithos (stone), and the Latin -iasis meaning "process" or "morbid condition"...
April 2018: Indian Journal of Surgery
Mateusz Falkowski, Mateusz Maciejczyk, Tomasz Koprowicz, Bożena Mikołuć, Anna Milewska, Anna Zalewska, Halina Car
Whey protein concentrate (WPC) is characterized by powerful antioxidant properties, but its effect on redox homeostasis of salivary glands of aging organisms is still unknown. In this study, we are the first to evaluate the antioxidant barrier of salivary glands of 14-month Wistar rats fed WPC-80. Total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT) as well as concentrations of reduced glutathione (GSH) are estimated in the submandibular and parotid glands of rats administered WPC-80 intragastrically for a period of 7 and 14 days...
June 17, 2018: Nutrients
T Mukaibo, T Munemasa, C Masaki, C Y Cui, J E Melvin
Mutations in the ectodysplasin A gene ( EDA) cause X-LHED (X-linked hypohidrotic ectodermal dysplasia), the most common human form of ectodermal dysplasia. Defective EDA signaling is linked to hypoplastic development of epithelial tissues, resulting in hypotrichosis, hypodontia, hypohidrosis, and xerostomia. The primary objective of the present study was to better understand the salivary gland dysfunction associated with ectodermal dysplasia using the analogous murine disorder. The salivary flow rate and ion composition of the 3 major salivary glands were determined in adult Eda-deficient Tabby hemizygous male (Ta/Y) and heterozygous female (Ta/X) mice...
June 1, 2018: Journal of Dental Research
Jeroen Vinke, Hans J Kaper, Arjan Vissink, Prashant K Sharma
Advances in medical research has resulted in successful treatment of many life-threatening infectious diseases as well as autoimmune and lifestyle-related diseases, increasing life-expectancy of both the developed and developing world. As a result of a growing ageing population, the focus has also turned on chronic diseases which seriously affect the quality of older patient life. Xerostomia (dry mouth) is one such condition, which leads to bad oral health and difficulty in consumption of dry foods and speech...
June 14, 2018: Scientific Reports
Federico Biglioli, Alessandro Lozza, Giacomo Colletti, Fabiana Allevi
Lingual nerve (LN) injury is one of the most serious consequences of oral surgery. Prompt microsurgical reconstruction of the nerve can alleviate most of those symptoms leading to satisfactory functional recovery.Thirty-five patients with partial to complete LN injury underwent surgery in the period between January 2006 and May 2015. All patients underwent a preoperative clinical and neurological evaluation with the assessment of lingual tactile and pain sensory thresholds and masseteric inhibitory reflex.All patients underwent explorative surgery and direct microneurorrhaphy of distal and proximal stumps in case of complete lesion, while the removal of traumatic neuroma and the following microneurorrhaphy of distal and proximal stumps of the injured nerve was performed in case of incomplete lesion...
June 11, 2018: Journal of Craniofacial Surgery
Naveen B Shivanand, Mihir T Mohan, Shawn T Joseph
Reconstruction of floor of the mouth and ventral surface of the tongue defects can be challenging because inadequate correction can lead to mobility restriction of the tongue and resultant impairment of speech and swallowing. Ideal flap should be pliable, provide adequate bulk, be easy to harvest, and cosmetically acceptable. Commonly used ipsilateral facial artery-based myomucosal flaps may not be ideal if facial vessels need resection. We share our experience in a case of simultaneous primary mucoepidermoid carcinoma of right submandibular and sublingual glands, with a postsurgical defect involving floor of the mouth and ventral surface of the tongue, reconstructed with islanded facial artery myomucosal flap raised from left buccal mucosa and tunneled into the right floor of the mouth defect medial to mandible...
June 2018: Craniomaxillofacial Trauma & Reconstruction
Abeer Shaalan, Guy Carpenter, Gordon Proctor
AIM: Inducible nitric oxide synthase (iNOS) is a key regulator of the innate immune system. The aim of the current study was to explore whether innate immune-mediated iNOS and reactive nitrogen species acutely perturb acinar cell physiology and calcium homeostasis of exocrine salivary tissues. METHODS: Innate immunity in the submandibular gland of C57BL/6 mice was locally activated via intraductal retrograde infusion of polyinosinic:polycytidylic acid (poly (I:C)...
June 7, 2018: Nitric Oxide: Biology and Chemistry
Jiannis Hajiioannou, Eleni Sioka, Ourania Koukoura, John Bizakis
Subcutaneous emphysema to the neck represents a rare entity mainly derived from iatrogenic and traumatic origin. We report a case of a subcutaneous emphysema resulted from an intraoral injury aiming to emphasise the significance of precise medical history to identify an unlikely mechanism. A 40-year-old female patient was presented with subcutaneous emphysema extending from the region underneath the left eye up to the submandibular area of the neck. The patient complained of painful, swollen neck. The patient mentioned that the symptoms began after an intraoral injury accidentally caused by the use of a high-pressure water jet device meant for car wash...
June 8, 2018: BMJ Case Reports
Brittney M Clark, Matthew L Carlson, Jamie J Van Gompel, Matthew A Warner
No abstract text is available yet for this article.
June 5, 2018: Canadian Journal of Anaesthesia, Journal Canadien D'anesthésie
Ricardo Coringa, Eduardo Martins de Sousa, Juliana Nunes Botelho, Rafael Soares Diniz, Joicy Cortez de Sá, Maria Carmen Fontoura Nogueira da Cruz, Marco Aurelio Beninni Paschoal, Letícia Machado Gonçalves
Despite their demonstrated biocompatibility and osteogenic properties, oyster shells have been reported as a potential alternative to other commonly used materials for bone substitution. This study evaluated whether an experimental bone substitute (EBS) made from a typical oyster shell of Northeastern Brazil (Crassostrea rhizophora) has effects on bone development using an animal model. Oysters were collected from a biologically assisted vivarium, and their inner layer was used for preparing an EBS. Chemical and surface characterization of EBS was performed using Individually Coupled Plasma Optical Emission Spectrometry (ICP-OES) and Scanning Electron Microscope (SEM), respectively...
2018: PloS One
Di-Kan Wang, Si-En Zhang, Yu-Xiong Su, Guang-Sen Zheng, Wei-Fa Yang, Gui-Qing Liao
PURPOSE: Keratoconjunctivitis sicca (KCS) is a relatively common disease that results in discomfort, tear film instability, visual impairment, and ocular surface damage. Artificial tear substitutes may be suitable for the treatment of mild KCS, but no effective treatment currently exists for severe KCS. Therefore, this study evaluated the effectiveness of autologous microvascular submandibular gland transplantation in the treatment of severe KCS. PATIENTS AND METHODS: A total of 61 eyes (56 patients) with severe KCS were treated with autologous submandibular gland transplantation from June 2002 to June 2017...
June 2, 2018: Journal of Oral and Maxillofacial Surgery
Wided Kouidhi, Raymond Bergès, Gaetan Drouin, Catherine Desmetz, Jacques Auger, Michèle El May, Marie Chantal Canivenc-Lavier
OBJECTIVES: We previously reported that maternal exposure to genistein, vinclozolin, ingested alone or in combination, affects submandibular salivary glands of rats' offspring's. Here, we investigated the responsiveness of submandibular gland when such xenohormone exposure occurs later in life. MATERIALS AND METHODS: Chemicals were given orally to male and female Wistar rats (1mg/kg body weight per day), from weaning to adulthood. Submandibular glands and plasma were collected at postnatal day 100 for histological and molecular analysis...
June 4, 2018: Oral Diseases
Yeni H Yücel, Kirsten Cardinell, Shireen Khattak, Xun Zhou, Michael Lapinski, Fang Cheng, Neeru Gupta
Purpose: To visualize and quantify lymphatic drainage of aqueous humor from the eye to cervical lymph nodes in the dynamic state. Methods: A near-infrared tracer was injected into the right eye anterior chamber of 10 mice under general anesthesia. Mice were imaged with photoacoustic tomography before and 20 minutes, 2, 4, and 6 hours after injection. Tracer signal intensity was measured in both eyes and right and left neck lymph nodes at every time point and signal intensity slopes were calculated...
June 1, 2018: Investigative Ophthalmology & Visual Science
Paul G Werthmann, Lothar Kindermann, Gunver S Kienle
BACKGROUND: Merkel cell carcinoma (MCC) is a rare, aggressive, neuroendocrine skin tumor with frequent local recurrence, lymph node involvement, distant metastasis, and a high mortality rate. Viscum album extracts (VAE) are a widely used adjunct in cancer treatment and show cytotoxic and immune-modulating effects. CASE PRESENTATION: A 64-year-old woman was diagnosed with a MCC of the left forearm. In the following course of 21 years, she experienced 4 episodes of lymph node relapse (axillary, submandibular, axillary, clavicular)...
June 2018: Complementary Therapies in Medicine
Xue-Ming Zhang, Yan Huang, Kuo Zhang, Ling-Han Qu, Xin Cong, Jia-Zeng Su, Li-Ling Wu, Guang-Yan Yu, Yan Zhang
Tight junction (TJ) proteins play a dynamic role in paracellular fluid transport in salivary gland epithelia. Most TJ studies are carried out in mice and rats. However, the morphology of rodent salivary glands differs from that of human glands. This study aimed to compare the histological features and the expression pattern of TJ proteins in porcine salivary glands with those of human and mouse. The results showed that porcine parotid glands were pure serous glands. Submandibular glands (SMGs) were serous acinar cell-predominated mixed glands, whereas sublingual glands were mucous acinar cell-predominated...
May 31, 2018: Journal of Anatomy
Joo Wan Kim, Jae-Suk Choi, Du Jin Seol, Jai Jun Choung, Sae Kwang Ku
Aim: Kuseonwangdogo is a traditional Korean immunomodulatory polyherbal prescription. However, there are no systemic findings on its complex immunomodulatory effects on in vivo models. In this study, we observed the immunomodulatory effects of Kuseonwangdogo-based mixed herbal formula aqueous extracts (MHFe) on cyclophosphamide- (CPA-) induced immunosuppression mouse model. Methods: In total, 60 male 6-week-old ICR mice (10 mice/group) were selected based on body weight 24 h after the second CPA treatment and used in this experiment...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Rose Mark, Swithin Song, Peter Mark
Descending necrotising mediastinitis (DNM) is an uncommon clinical entity which may arise secondary to primary odontogenic or neck infection in susceptible patients. Infection may spread contiguously via the alar or 'danger' space, a potential anatomical space posterior to the true retropharyngeal space.1 Spread of infection to the mediastinum almost always necessitates urgent cardiothoracic surgical intervention.2-4 This case report describes a male patient whose clinical deterioration following the diagnosis of submandibular abscess was investigated with CT imaging of the chest, where a diagnosis of DNM was made...
May 30, 2018: BMJ Case Reports
Benedetta Amato, Maria Vitale, Maria Teresa Capucchio, Michele Fiasconaro, Elisabetta Gerace, Roberto Puleio, Vincenzo Di Marco Lo Presti
During a routine abattoir inspection of pig carcasses and control activities of hunted wildlife in 2013, 118 large white pigs Sus scrofa domesticus, 474 Nebrodi black pigs and 135 wild boars Sus scrofa scrofa were submitted to anatomopathological examination to evaluate the presence of tuberculosis-like lesions. Localized and generalized granulomatous lesions were detected with a prevalence of about 25% in large white pigs, 13% in Nebrodi black pigs and 8.15% in wild boars. Localized lesions involved mainly the submandibular lymph nodes, but when the disease was spread throughout the body, the inner organs and, also, in some cases, udders and/or bones were injured...
May 31, 2018: Advances in Experimental Medicine and Biology
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