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Glucoamylase

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https://www.readbyqxmd.com/read/28780468/design-and-synthesis-of-2-6-di-substituted-phenyl-thiazolo-3-2-b-1-2-4-triazoles-as-%C3%AE-glucosidase-and-%C3%AE-amylase-inhibitors-co-relative-pharmacokinetics-and-3d-qsar-and-risk-analysis
#1
Pervaiz Ali Channar, Aamer Saeed, Fayaz Ali Larik, Sajid Rashid, Qaiser Iqbal, Maryam Rozi, Saima Younis, Jamaluddin Mahar
Ten fused heterocyclic derivatives bearing the 2,6-di(subsituted phenyl)thiazolo[3,2-b]-1,2,4-triazoles as central rings were synthesized and structures of the compounds were established by analytical and spectral data using FTIR, EI-MS, (1)H NMR and (13)C NMR techniques. In vitro inhibitory activities of synthesized compounds on α-amylase, α-glucosidase and α-burylcholinesterase (α-BuChE) were evaluated using a purified enzyme assays. Compound 5c demonstrated strong and selective α-amylase inhibitory activity (IC50=1...
August 3, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28747585/amylolytic-fungi-in-starter-cakes-for-rice-beer-production
#2
Arup Jyoti Das, Tatsuro Miyaji, Sankar Chandra Deka
Two types of starter cakes, viz. amou and perok-kushi, used in the production of rice beer in Assam, India, by the Bodo and Deori communities, respectively, were used for the isolation of amylolytic fungi. Based on the sequencing of their internal transcribed spacer (ITS) regions the fungi were identified as Amylomyces rouxii and Rhizopus oryzae, and given the strain names TU460 and TU465, respectively. Both the strains showed the ability to degrade and saccharify starch. The glucoamylase activity was considerably high in A...
July 27, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28743272/establishment-of-neurospora-crassa-as-a-host-for-heterologous-protein-production-using-a-human-antibody-fragment-as-a-model-product
#3
David Havlik, Ulrike Brandt, Kathrin Bohle, André Fleißner
BACKGROUND: Filamentous fungi are commonly used as production hosts for bulk enzymes in biotechnological applications. Their robust and quick growth combined with their ability to secrete large amounts of protein directly into the culture medium makes fungi appealing organisms for the generation of novel production systems. The red bread mold Neurospora crassa has long been established as a model system in basic research. It can be very easily genetically manipulated and a wealth of molecular tools and mutants are available...
July 25, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28727742/physical-structure-and-absorption-properties-of-tailor-made-porous-starch-granules-produced-by-selected-amylolytic-enzymes
#4
Yi-Seul Jung, Byung-Hoo Lee, Sang-Ho Yoo
Porous starch granules (PSGs) with various pores and cavity sizes were prepared by amylolysis enzymes. The greatest hydrolysis rate on corn starch granule was observed with α-amylase, followed by gluco- and β-amylases. Temperature increase enhanced glucoamylase reaction rate more drastically than other enzyme treatments. Final hydrolysis level with glucoamylase reached to 66.9%, close to 67.5% of α-amylolysis. The α-amylase-treated PSGs displayed the greatest pore size and ratio of cavity-to-granule diameters...
2017: PloS One
https://www.readbyqxmd.com/read/28726400/quinoliniumolate-and-2h-1-2-3-triazole-derivatives-from-the-stems-of-paramignya-trimera-and-their-%C3%AE-glucosidase-inhibitory-activities-in-vitro-and-in-silico-studies
#5
Nhan T Nguyen, Phu H Dang, Ngoc X T Vu, Tho H Le, Mai T T Nguyen
From a CHCl3-soluble extract of the stems of Paramignya trimera, two new alkaloids, (E)-2-(prop-1-enyl)-N-methylquinolinium-4-olate (1) and (R)-2-ethylhexyl 2H-1,2,3-triazole-4-carboxylate (2), were isolated. Their structures were elucidated based on the spectroscopic data interpretation. Compound 2 possesses α-glucosidase inhibitory activity, with an IC50 value of 137.9 μM. Molecular docking studies of 1 and 2 with human maltase-glucoamylase (MGAM) were performed for the first time; thus, the 2,3-diH(+)-1H-1,2,3-triazolium cation (2i) showed good interactions with both MGAM-N (2QMJ) and -C (3TOP) terminal subunits...
July 20, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28724263/mutagenesis-and-characterization-of-a-bacillus-amyloliquefaciens-strain-for-cinnamomum-camphora-seed-kernel-oil-extraction-by-aqueous-enzymatic-method
#6
Cheng Zeng, Rongbin Zhao, Maomao Ma, Zheling Zeng, Deming Gong
The purpose of the present study was to increase the proteinase activity of the strain NCU116 by combining ultraviolet irradiation and N-methyl-N'-nitro-N-nitroso guanidine treatment, in order to enhance the efficiency of Cinnamomum camphora seed kernel oil (CCSKO) extraction by aqueous enzymatic method (AEM). The mutated strain, designated as NCU116-1, was screened out by the ratio of hydrolytic zone diameter to colony diameter on skim milk plate. The proteinase activity (9116.1 U/ml) of NCU116-1 was increased by 31...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28660456/sorghum-husk-biomass-as-a-potential-substrate-for-production-of-cellulolytic-and-xylanolytic-enzymes-by-nocardiopsis-sp-knu
#7
Siddheshwar D Kshirsagar, Bhumika N Bhalkar, Pankajkumar R Waghmare, Ganesh D Saratale, Rijuta G Saratale, Sanjay P Govindwar
Nocardiopsis sp. KNU was found to degrade various lignocellulosic waste materials, namely, sorghum husk, sugarcane tops and leaves, wheat straw, and rice husk very efficiently. The strain was found to produce high amounts of cellulase and hemicellulase. Augmentation of cotton seed cake as an organic nitrogen source revealed inductions in activities of endoglucanase, glucoamylase, and xylanase up to 70.03, 447.89, and 275.10 U/ml, respectively. Nonionic surfactant Tween-80 addition was found to enhance the activity of endoglucanase enzyme...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28637248/expression-and-comparison-of-codon-optimised-aspergillus-tubingensis-amylase-variants-in-saccharomyces-cerevisiae
#8
Rosemary A Cripwell, Shaunita H Rose, Willem H van Zyl
The expression of codon optimised genes is a popular genetic engineering approach for the production of industrially relevant proteins. This study investigates and compares the expression of codon optimised and codon adapted amylase variants. The Aspergillus tubingensis raw starch hydrolysing α-amylase (amyA) and glucoamylase (glaA) encoding genes were redesigned using synonymous codons and expressed in S. cerevisiae Y294. Codon optimisation to favour S. cerevisiae codon bias resulted in a decrease in extracellular enzyme activity of 72% (30...
June 15, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28568243/rethinking-the-starch-digestion-hypothesis-for-amy1-copy-number-variation-in-humans
#9
EDITORIAL
Catalina I Fernández, Andrea S Wiley
Alpha-amylase exists across taxonomic kingdoms with a deep evolutionary history of gene duplications that resulted in several α-amylase paralogs. Copy number variation (CNV) in the salivary α-amylase gene (AMY1) exists in many taxa, but among primates, humans appear to have higher average AMY1 copies than nonhuman primates. Additionally, AMY1 CNV in humans has been associated with starch content of diets, and one known function of α-amylase is its involvement in starch digestion. Thus high AMY1 CNV is considered to result from selection favoring more efficient starch digestion in the Homo lineage...
August 2017: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/28527863/over-production-of-fermentable-sugar-for-bioethanol-production-from-carbohydrate-rich-malaysian-food-waste-via-sequential-acid-enzymatic-hydrolysis-pretreatment
#10
Halimatun Saadiah Hafid, Abdul Rahman Nor 'Aini, Mohd Noriznan Mokhtar, Ahmad Tarmezee Talib, Azhari Samsu Baharuddin, Md Shah Umi Kalsom
In Malaysia, the amount of food waste produced is estimated at approximately 70% of total municipal solid waste generated and characterised by high amount of carbohydrate polymers such as starch, cellulose, and sugars. Considering the beneficial organic fraction contained, its utilization as an alternative substrate specifically for bioethanol production has receiving more attention. However, the sustainable production of bioethanol from food waste is linked to the efficient pretreatment needed for higher production of fermentable sugar prior to fermentation...
May 17, 2017: Waste Management
https://www.readbyqxmd.com/read/28514399/-disaccharidase-deficiency-and-functional-bowel-diseases
#11
A I Parfenov, O V Akhmadullina, E A Sabelnikova, N I Belostotsky, M V Kirova, S G Khomeriki, S V Bykova
AIM: To elucidate the role of intestinal carbohydrases (glucoamylase, maltase, sucrose, and lactase) in the etiology and pathogenesis of functional bowel diseases (FBD). SUBJECTS AND METHODS: 74 patients (36 men and 38 women) aged 18 to 50 years with FBD were examined. According to Rome IV criteria (2016), there was diarrhea-predominant irritable bowel syndrome (IBS) in 21 patients, functional diarrhea (FD) in 33, constipation-predominant IBS in 6, functional constipation (FC) in 4, and mixed IBS in 10...
2017: Terapevticheskiĭ Arkhiv
https://www.readbyqxmd.com/read/28483534/comparative-secretomic-analysis-of-lignocellulose-degradation-by-lentinula-edodes-grown-on-microcrystalline-cellulose-lignosulfonate-and-glucose
#12
Yingli Cai, Yuhua Gong, Wei Liu, Yue Hu, Lianfu Chen, Lianlian Yan, Yan Zhou, Yinbing Bian
Lentinula edodes has the potential to degrade woody and nonwoody lignocellulosic biomass. However, the mechanism of lignocellulose degradation by L. edodes is unclear. The aim of this work is to explore the profiling of soluble secreted proteins involved in lignocellulose degradation in L. edodes. For that, we compared the secretomes of L. edodes grown on microcrystalline cellulose, cellulose with lignosulfonate and glucose. Based on nanoliquid chromatography coupled with tandem mass spectrometry of whole-protein hydrolysate, 230 proteins were identified...
June 23, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28481023/dynamic-modulation-of-enzyme-activity-by-near-infrared-light
#13
Changping Wang, Qiang Zhang, Xinyu Wang, Hong Chang, Sanjun Zhang, Yuankai Tang, Jianhua Xu, Ruijuan Qi, Yiyun Cheng
Engineering near-infrared (NIR) light-sensitive enzymes remains a huge challenge. A photothermal effect-associated method is developed for tailoring the enzymatic activity of enzymes by exposure to NIR light. An ultrasmall platinum nanoparticle was anchored in an enzyme to generate local heating upon NIR irradiation, which enhanced the enzyme activity without increasing bulk temperature. Following NIR irradiation, the enzyme activity was tailored rapidly and reversibly, and was modulated by varying laser power density and irradiation time...
May 8, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28465177/simultaneous-production-of-both-high-molecular-weight-pullulan-and-oligosaccharides-by-aureobasdium-melanogenum-p16-isolated-from-a-mangrove-ecosystem
#14
Nan-Nan Liu, Zhe Chi, Qin-Qing Wang, Jiang Hong, Guang-Lei Liu, Zhong Hu, Zhen-Ming Chi
After the compositional change of a pullulan production medium, a molecular weight (Mw) of the pullulan produced by Aureobasidium melanogenum P16 was 2.32×10(6) and a pullulan titer was 44.4g/L while a Mw of the pullulan produced by A. melanogenum P16 grown in the initial medium was only 3.47×10(5) and a pullulan titer was 65.3g/L. The increased Mw of the pullulan was due to the decreased activities of α-amylase, glucoamylase and pullulanase while the decreased pullulan titer was related to the decreased transcriptional levels of the genes encoding 6-P-glucose kinase, glucosyltransferase, α-phosphoglucose mutase, UDPG-pyrophosphorylase and pullulan synthetase...
September 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28464820/purification-and-characterization-of-a-novel-cold-adapted-fungal-glucoamylase
#15
Mario Carrasco, Jennifer Alcaíno, Víctor Cifuentes, Marcelo Baeza
BACKGROUND: Amylases are used in various industrial processes and a key requirement for the efficiency of these processes is the use of enzymes with high catalytic activity at ambient temperature. Unfortunately, most amylases isolated from bacteria and filamentous fungi have optimal activity above 45 °C and low pH. For example, the most commonly used industrial glucoamylases, a type of amylase that degrades starch to glucose, are produced by Aspergillus strains displaying optimal activities at 45-60 °C...
May 2, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28455587/comprehensive-analysis-of-fungal-diversity-and-enzyme-activity-in-nuruk-a-korean-fermenting-starter-for-acquiring-useful-fungi
#16
Emily Carroll, Tran Ngoc Trinh, Hokyoung Son, Yin-Won Lee, Jeong-Ah Seo
Nuruk is a fermenting starter that is involved in the production of alcoholic beverages, and has been used in South Korea for a very long time. To analyze the fungal diversity, we collected a total of 59 nuruk samples from several companies and persons in 2013 to 2014, and obtained 364 isolates. All of the single isolated fungi were identified, both morphologically and molecularly, based on the sequences of ribosomal RNA gene [18S, ITS1-5.8S-ITS2, and 26S (D1/D2 region)]. In 46 nuruk samples out of 59 (78%), Saccharomycopsis fibuligera, a dimorphic yeast, was most frequently isolated...
May 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28443839/phenolic-compounds-increase-the-transcription-of-mouse-intestinal-maltase-glucoamylase-and-sucrase-isomaltase
#17
Meric Simsek, Roberto Quezada-Calvillo, Buford L Nichols, Bruce R Hamaker
Diverse natural phenolic compounds show inhibition activity of intestinal α-glucosidases, which may constitute the molecular basis for their ability to control systemic glycemia. Additionally, phenolics can modify mRNA expression for proteins involved in nutritional, metabolic or immune processes. To explore the possibility that phenolics can regulate the mRNA expression, enzymatic activity, and protein synthesis/processing of intestinal Maltase-Glucoamylase (MGAM) and Sucrase-Isomaltase (SI), small intestinal explants from Balb/c mice were cultured for 24 h in the presence or absence of gallic acid, caffeic acid, and (+)-catechin at 0...
May 24, 2017: Food & Function
https://www.readbyqxmd.com/read/28412339/purification-and-functional-properties-of-a-novel-glucoamylase-activated-by-manganese-and-lead-produced-by-aspergillus-japonicus
#18
Thiago Machado Pasin, Vivian Machado Benassi, Paulo Ricardo Heinen, André Ricardo de Lima Damasio, Mariana Cereia, João Atílio Jorge, Maria de Lourdes Teixeira de Moraes Polizeli
Microbial amylases are used to produce ethanol, glucose and can be applied in textiles products, detergents and other industries. This study aimed to determine the best carbon source concentration to induce the amylase production by A. japonicus, and its purification and biochemical characterization. For that, this fungus was cultivated in Khanna medium, pH 5.5, for 4 days, at 25°C, in static condition, supplemented with potato starch and maltose in different concentrations. The fungal crude enzymatic extract was purified in a unique elution in DEAE-cellulose column and the molecular mass was determined as 72kDa...
September 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28389849/high-efficient-production-of-citric-acid-by-aspergillus-niger-from-high-concentration-of-substrate-based-on-the-staged-addition-glucoamylase-strategy
#19
Baoshi Wang, Hua Li, Linghuan Zhu, Fengling Tan, Youran Li, Liang Zhang, Zhongyang Ding, Guiyang Shi
Citric acid (CA), an important platform-compound, has attracted much attention because of its broad applications and huge market demand. To solve high residual sugar at the fermentation end, we put forwarded strategy of pre-saccharification and then fermentation. Results showed that the residual total sugar decreased by 10.4% and the productivity increased by 4.0% and initially high glucose inhibited cell growth. Furthermore, commercial glucoamylase with high low-pH stability was proposed to staged-add in the fermentation process, which timely compensated enzyme loss, ensuring the glucose supply rate...
April 7, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28371716/bacillus-licheniformis-affects-the-microbial-community-and-metabolic-profile-in-the-spontaneous-fermentation-of-daqu-starter-for-chinese-liquor-making
#20
Peng Wang, Qun Wu, Xuejian Jiang, Zhiqiang Wang, Jingli Tang, Yan Xu
Chinese liquor is produced from spontaneous fermentation starter (Daqu) that provides the microbes, enzymes and flavors for liquor fermentation. To improve the flavor character of Daqu, we inoculated Bacillus licheniformis and studied the effect of this strain on the community structure and metabolic profile in Daqu fermentation. The microbial relative abundance changed after the inoculation, including the increase in Bacillus, Clavispora and Aspergillus, and the decrease in Pichia, Saccharomycopsis and some other genera...
March 14, 2017: International Journal of Food Microbiology
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