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Glucoamylase

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https://www.readbyqxmd.com/read/29657410/the-effect-of-purified-quercus-cortex-extract-on-biochemical-parameters-of-organism-and-productivity-of-healthy-broiler-chickens
#1
Galimzhan Kalihanovich Duskaev, Nadezhda Mihajlovna Kazachkova, Alexander Sergeevich Ushakov, Baer Serekpaevich Nurzhanov, Albert Farhitdinovich Rysaev
Aim: Modern methods of producing poultry meat without the use of antibiotics are known, and it is possible to achieve the desired conditions, including the use of herbal preparations. In addition, it is known that metabolites of medicinal plants are inhibitors of the quorum sensing system in bacteria. The aim of the present study was to determine the effect of Quercus cortex extract in a reduced dose on the productivity and body state of healthy chicken broilers. Materials and Methods: For the experiment, 120 heads of 7-day-old healthy broiler chickens were selected, and they were divided into four groups (n=30, 3 replicates of 10 birds in each group) by the analog method...
February 2018: Veterinary World
https://www.readbyqxmd.com/read/29644595/enhanced-bioethanol-production-from-potato-peel-waste-via-consolidated-bioprocessing-with-statistically-optimized-medium
#2
Tahmina Hossain, Abdul Bathen Miah, Siraje Arif Mahmud, Abdullah-Al- Mahin
In this study, an extensive screening was undertaken to isolate some amylolytic microorganisms capable of producing bioethanol from starchy biomass through Consolidated Bioprocessing (CBP). A total of 28 amylolytic microorganisms were isolated, from which 5 isolates were selected based on high α-amylase and glucoamylase activities and identified as Candida wangnamkhiaoensis, Hyphopichia pseudoburtonii (2 isolates), Wickerhamia sp., and Streptomyces drozdowiczii based on 26S rDNA and 16S rDNA sequencing. Wickerhamia sp...
April 12, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29622213/efficient-transformation-of-sucrose-into-high-pullulan-concentrations-by-aureobasidium-melanogenum-tn1-2-isolated-from-a-natural-honey
#3
Hong Jiang, Si-Jia Xue, Yan-Feng Li, Guang-Lei Liu, Zhen-Ming Chi, Zhong Hu, Zhe Chi
A very high pullulan producing yeast-like fungus, Aureobasidium melanogenum TN1-2 isolated from a natural honey was found to be able to produce 97.0 g/L of pullulan from 140.0 g/L sucrose at a flask level while it could yield 114.0 g/L of pullulan within 134 h during a 10-liter fermentation, the yield was 0.81 g/g and the productivity was 0.86 g/L/h. The high ability to biosynthesize pullulan by this yeast-like fungal strain TN1-2 was related to high glucosyltransferase activity, high phosphofructo-2-kinase activity, high content of its cellular glycerol and low glucose repressor...
August 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29605944/the-glaa-signal-peptide-substantially-increases-the-expression-and-secretion-of-%C3%AE-galactosidase-in-aspergillus-niger
#4
Yue Xu, Yan-Hui Wang, Tian-Qi Liu, Hui Zhang, He Zhang, Jie Li
OBJECTIVE: α-Galactosidases are widely used in many fields. It is necessary to improve the production of enzymes through microbiological processes. The aim of this study was to construct recombinant Aspergillus niger strains with high α-galactosidase production. RESULTS: Two recombinant A. niger strains were constructed: AB and AGB. The recombinant AB strain contained the α-galactosidase aglB gene from A. niger with its native AglB signal peptide regulated by the glucoamylase promoter...
March 31, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29603462/structural-basis-for-the-flexible-recognition-of-%C3%AE-glucan-substrates-by-bacteroides-thetaiotaomicron-susg
#5
Gregory Arnal, Darrell W Cockburn, Harry Brumer, Nicole M Koropatkin
Bacteria that reside in the mammalian intestinal tract efficiently hydrolyze dietary carbohydrates, including starch, that escape digestion in the small intestine. Starch is an abundant dietary carbohydrate comprised of α1,4 and α1,6 linked glucose, yet mammalian intestinal glucoamylases cannot effectively hydrolyze starch that has frequent α1,6 branching as these structures hinder recognition and processing by α1,4-specific amylases. Here we present the structure of the cell surface amylase SusG from Bacteroides thetaiotaomicron complexed with a mixed linkage amylosaccharide generated from transglycosylation during crystallization...
March 30, 2018: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29565725/potential-application-of-a-low-viscosity-and-high-transparency-xanthan-gum-produced-from-xanthomonas-campestris-cctcc-m2015714-in-foods
#6
Zichao Wang, Libo Yang, Jianrong Wu, Hongtao Zhang, Li Zhu, Xiaobei Zhan
Xanthan gum is commonly used as thickener in food industry, while the usage of xanthan gum as dietary fiber is restricted for its low additive volume. Herein, the potential use of a low-viscosity and high-transparency xanthan gum as dietary fiber was evaluated in vitro . This new xanthan shows better transparency and faster dissolution rate than most commercial products, and its viscosity increases along with the treatment of freeze-thaw cycles at -20 °C. Moreover, this new xanthan can absorb heavy metals (Pb, Cd, Cu) and retard starch digestion by glucoamylase...
March 22, 2018: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/29548257/mechanistic-pathway-on-human-%C3%AE-glucosidase-maltase-glucoamylase-unveiled-by-qm-mm-calculations
#7
Natércia Fernandes Brás, Diogo Santos-Martins, Pedro A Fernandes, Maria João Ramos
The excessive consumption of starch in human diets is associated with highly prevalent chronic metabolic diseases such as type 2 diabetes and obesity. α-glucosidase enzymes contribute for the digestion of starch into glucose and are thus attractive therapeutic targets for diabetes. Given that the active sites of the various families of α-glucosidases have different sizes and structural features, atomistic descriptions of the catalytic mechanisms of these enzymes can support the development of potent and selective new inhibitors...
March 16, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29545946/supplementation-of-amylase-combined-with-glucoamylase-or-protease-changes-intestinal-microbiota-diversity-and-benefits-for-broilers-fed-a-diet-of-newly-harvested-corn
#8
Dafei Yin, Xiaonan Yin, Xingyu Wang, Zhao Lei, Maofei Wang, Yuming Guo, Samuel E Aggrey, Wei Nie, Jianmin Yuan
Background: The effect of amylases combined with exogenous carbohydrase and protease in a newly harvested corn diet on starch digestibility, intestine health and cecal microbiota was investigated in broiler chickens. Methods: Two hunderd and eighty-eight 5-day-old female chickens were randomly divided into six treatments: a newly harvested corn-soybean meal diet (control); control supplemented with 1,500 U/g α-amylase (Enzyme A); Enzyme A + 300 U/g amylopectase + 20,000 U/g glucoamylase (Enzyme B); Enzyme B + protease 10,000 U/g (Enzyme C); Enzyme C + xylanase 15,000 U/g (Enzyme D); and Enzyme D + cellulase 200 U/g + pectinase 1,000 U/g (Enzyme E)...
2018: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29541080/novel-bioengineered-cassava-expressing-an-archaeal-starch-degradation-system-and-a-bacterial-adp-glucose-pyrophosphorylase-for-starch-self-digestibility-and-yield-increase
#9
Ayalew Ligaba-Osena, Jenna Jones, Emmanuel Donkor, Sanjeev Chandrayan, Farris Pole, Chang-Hao Wu, Claire Vieille, Michael W W Adams, Bertrand B Hankoua
To address national and global low-carbon fuel targets, there is great interest in alternative plant species such as cassava ( Manihot esculenta ), which are high-yielding, resilient, and are easily converted to fuels using the existing technology. In this study the genes encoding hyperthermophilic archaeal starch-hydrolyzing enzymes, α-amylase and amylopullulanase from Pyrococcus furiosus and glucoamylase from Sulfolobus solfataricus , together with the gene encoding a modified ADP-glucose pyrophosphorylase ( glgC ) from Escherichia coli , were simultaneously expressed in cassava roots to enhance starch accumulation and its subsequent hydrolysis to sugar...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29518233/saccharomyces-cerevisiae-variety-diastaticus-friend-or-foe-spoilage-potential-and-brewing-ability-of-different-saccharomyces-cerevisiae-variety-diastaticus-yeast-isolates-by-genetic-phenotypic-and-physiological-characterization
#10
Tim Meier-Dörnberg, Oliver Ingo Kory, Fritz Jacob, Maximilian Michel, Mathias Hutzler
Saccharomyces cerevisiae variety diastaticus is generally considered to be an obligatory spoilage microorganism and spoilage yeast in beer and beer-mixed beverages (Folz, Hofmann and Stahl 2011; Hutzler et al. 2012). Their super-attenuating ability causes increased carbon dioxide concentrations, beer gushing and potential bottle explosion along with changes in flavor, sedimentation and increased turbidity. This research shows clear differences in the super-attenuating properties of S. cerevisiae var. diastaticus yeast strains and their potential for industrial brewing applications...
March 6, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29515964/bio-ethanol-production-from-waste-biomass-of-pogonatherum-crinitum-phytoremediator-an-eco-friendly-strategy-for-renewable-energy
#11
Pankajkumar R Waghmare, Anuprita D Watharkar, Byong-Hun Jeon, Sanjay P Govindwar
In this study, we have described three steps to produce ethanol from Pogonatherum crinitum , which was derived after the treatment of textile wastewater. (a) Production of biomass: biomass samples collected from a hydroponic P. crinitum phytoreactor treating dye textile effluents and augmented with Ca-alginate immobilized growth-promoting bacterium, Bacillus pumilus strain PgJ (consortium phytoreactor), and waste sorghum husks were collected and dried. Compositional analysis of biomass (consortium phytoreactor) showed that the concentration of cellulose, hemicelluloses and lignin was 42, 30 and 17%, respectively, whereas the biomass samples without the growth-promoting bacterium (normal phytoreactor) was slightly lower, 40, 29 and 16%, respectively...
March 2018: 3 Biotech
https://www.readbyqxmd.com/read/29495635/the-postprandial-anti-hyperglycemic-effect-of-pyridoxine-and-its-derivatives-using-in-vitro-and-in-vivo-animal-models
#12
Hyuk Hwa Kim, Yu-Ri Kang, Jung-Yun Lee, Hung-Bae Chang, Ki Won Lee, Emmanouil Apostolidis, Young-In Kwon
In the current study, we investigated the inhibitory activity of pyridoxine, pyridoxal, and pyridoxamine, against various digestive enzymes such as α-glucosidases, sucrase, maltase, and glucoamylase. Inhibition of these enzymes involved in the absorption of disaccharide can improve post-prandial hyperglycemia due to a carbohydrate-based diet. Pyridoxal (4.14 mg/mL of IC50 ) had the highest rat intestinal α-glucosidase inhibitory activity, followed by pyridoxamine and pyridoxine (4.85 and 5.02 mg/mL of IC50 , respectively)...
February 28, 2018: Nutrients
https://www.readbyqxmd.com/read/29492105/direct-and-highly-productive-conversion-of-cyanobacteria-arthrospira-platensis-to-ethanol-with-cacl-2-addition
#13
Shimpei Aikawa, Kentaro Inokuma, Satoshi Wakai, Kengo Sasaki, Chiaki Ogino, Jo-Shu Chang, Tomohisa Hasunuma, Akihiko Kondo
Background: The cyanobacterium Arthrospira platensis shows promise as a carbohydrate feedstock for biofuel production. The glycogen accumulated in A. platensis can be extracted by lysozyme-degrading the peptidoglycan layer of the bacterial cell walls. The extracted glycogen can be converted to ethanol through hydrolysis by amylolytic enzymes and fermentation by the yeast Saccharomyces cerevisiae . Thus, in the presence of lysozyme, a recombinant yeast expressing α-amylase and glucoamylase can convert A...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29476862/the-effect-of-ultrasound-on-the-properties-and-conformation-of-glucoamylase
#14
Huanmei Meng, Dan Li, Chuanhe Zhu
In this study, the effects of ultrasonic treatment on the enzyme activity of glucoamylase were investigated and the mechanism of the effect was explored by determining their conformational changes. The enzyme activity was increased by 21.07% over the control with ultrasound at 420 W for 10 min at 60 °C, and Vmax and Km values of glucoamylase were also increased. After ultrasonic treatment at 420 W, thermodynamic experiments of glucoamylase showed that Ea, ΔG, ΔH decreased but slight increase in ΔS was observed...
February 21, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29465310/dietary-starch-breakdown-product-sensing-mobilizes-and-apically-activates-%C3%AE-glucosidases-in-small-intestinal-enterocytes
#15
Mohammad Chegeni, Mahdi Amiri, Buford L Nichols, Hassan Y Naim, Bruce R Hamaker
Dietary starch is finally converted to glucose for absorption by the small intestine mucosal α-glucosidases (sucrase-isomaltase [SI] and maltase-glucoamylase), and control of this process has health implications. Here, the molecular mechanisms were analyzed associated with starch-triggered maturation and transport of SI. Biosynthetic pulse-chase in Caco-2 cells revealed that the high MW SI species (265 kDa) induced by maltose (an α-amylase starch digestion product) had a higher rate of early trafficking and maturation compared with a glucose-induced SI (245 kDa)...
February 20, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29458106/combi-metal-organic-framework-combi-mof-of-%C3%AE-amylase-and-glucoamylase-for-one-pot-starch-hydrolysis
#16
Manish Salgaonkar, Shamraja S Nadar, Virendra K Rathod
The multi-enzyme biocatalyst allows to run in vitro multi-step cascade reactions in a single pot. An efficient combi-metal organic frameworks (combi-MOF) of α-amylase and glucoamylase for one pot starch hydrolysis was constructed by mixing zinc acetate and 2‑methylimmidazole with enzyme mixture in one pot under biocompatible conditions. The prepared combi-MOF was characterized and analyzed by powder X-ray diffraction (PXRD), Fourier transform infrared (FT-IR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM)...
February 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29430363/optimization-of-gluco-amylase-production-from-aspergillus-spp-for-its-use-in-saccharification-of-liquefied-corn-starch
#17
Deepali Jain, Priya Katyal
Fungal gluco-amylase is required for the production of sugars from starchy substrates. Commercially available fungal gluco-amylase is quite costly which makes the process uneconomical. This study was undertaken to standardize physico-chemical parameters for optimum production of gluco-amylases from Aspergillus spp. Two fungal cultures, i.e., Aspergillus niger and Aspergillus terreus, were compared for gluco-amylase activity both under stationary and shake flask conditions. Among two fungal cultures, maximum gluco-amylase activity was shown by A...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29421697/a-new-entry-into-the-portfolio-of-%C3%AE-glucosidase-inhibitors-as-potent-therapeutics-for-type-2-diabetes-design-bioevaluation-and-one-pot-multi-component-synthesis-of-diamine-bridged-coumarinyl-oxadiazole-conjugates
#18
Madiha Kazmi, Sumera Zaib, Aliya Ibrar, Sayyeda Tayyeba Amjad, Zainab Shafique, Saifullah Mehsud, Aamer Saeed, Jamshed Iqbal, Imtiaz Khan
Diabetes mellitus (DM), a chronic multifarious metabolic disorder resulting from impaired glucose homeostasis has become one of the most challenging diseases with severe life threat to public health. The inhibition of α-glucosidase, a key carbohydrate hydrolyzing enzyme, could serve as one of the effective methodology in both preventing and treating diabetes through controlling the postprandial glucose levels and suppressing postprandial hyperglycemia. In this context, three series of diamine-bridged bis-coumarinyl oxadiazole conjugates were designed and synthesized by one-pot multi-component methodology...
January 16, 2018: Bioorganic Chemistry
https://www.readbyqxmd.com/read/29421509/morning-glory-resin-glycosides-as-%C3%AE-glucosidase-inhibitors-in%C3%A2-vitro-and-in-silico-analysis
#19
Daniel Rosas-Ramírez, Sonia Escandón-Rivera, Rogelio Pereda-Miranda
Twenty-seven individual resin glycosides from the morning glory family (Convolvulaceae) were evaluated for their α-glucosidase inhibitory potential. Four of these compounds displayed an inhibitory activity comparable to acarbose, which was used as a positive control. Molecular modeling studies performed by docking analysis were accomplished to predict that the active compounds and acarbose bind to the α-1,4-glucosidase enzyme catalytic site of MAL12 from the yeast Saccharomyces cerevisiae through stable hydrogen bonds primarily with the amino acid residues HIS279 and GLN322...
February 1, 2018: Phytochemistry
https://www.readbyqxmd.com/read/29414174/valorisation-of-food-and-beverage-waste-via-saccharification-for-sugars-recovery
#20
Tsz Him Kwan, Khai Lun Ong, Md Ariful Haque, Wing Hei Kwan, Sandeep Kulkarni, Carol Sze Ki Lin
Valorisation of mixed food and beverage (F&B) waste was studied for the recovery of sugars via saccharification. Glucoamylase and sucrase were employed to hydrolyse the starch and sucrose present in the mixed F&B waste because of the high cost-effectiveness for such recovery. The Michaelis-Menten kinetics model suggests that preservatives and additives in beverages did not inhibit glucoamylase and sucrase during saccharification. High levels of glucose (228.1 g L-1) and fructose (55.7 g L-1) were efficiently produced within 12 h at a solid-to-liquid ratio of 37...
January 31, 2018: Bioresource Technology
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