keyword
MENU ▼
Read by QxMD icon Read
search

Glucoamylase

keyword
https://www.readbyqxmd.com/read/29046501/characterization-of-amylolytic-enzyme-overproducing-mutant-of-aspergillus-luchuensis-obtained-by-ion-beam-mutagenesis
#1
Toshihiro Kojo, Chihiro Kadooka, Marisa Komohara, Shiori Onitsuka, Miya Tanimura, Yukiko Muroi, Shugo Kurazono, Yohei Shiraishi, Ken Oda, Kazuhiro Iwashita, Masahira Onoue, Kayu Okutsu, Yumiko Yoshizaki, Kazunori Takamine, Taiki Futagami, Kazuki Mori, Hisanori Tamaki
Aspergillus luchuensis is a kuro (black) koji fungus that has been used as a starch degrader for the awamori- and shochu-making industries in Japan. In this study, we investigated the effect of ion beam irradiation on A. luchuensis RIB2601 and obtained a high starch-degrading mutant strain U1. Strain U1 showed reduced growth rate, whereas it showed higher α-amylase, glucoamylase, and α-glucosidase activities on a mycelial mass basis than the wild type (wt) strain both on agar plates and in rice koji. In addition, strain U1 showed higher N-acetylglucosamine content in the cell wall and higher sensitivity to calcofluor white, suggesting a deficiency in cell wall composition...
October 17, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/29023000/application-of-response-surface-methodology-for-optimization-of-extracellular-glucoamylase-production-by-candida-guilliermondii
#2
Lagzouli Mohamed, Youssfi El Kettani, Aitounejjar Ali, Elyachioui Mohamed, Jadal Mohamed
OBJECTIVE: Glucoamylase is among the most important enzymes in biotechnology. The present study aims to determine better conditions for growth and glucoamylase production by Candida guilliermondii and to reduce the overall cost of the medium using Box-Behnken design with one central point and response surface methodology. METHODOLOGY: Box-Behnken factorial design based on three levels was carried out to obtain optimal medium combination of five independent variables such as initial pH, soluble starch, CH4N2O, yeast extract and MgSO4...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/28994704/characterization-of-mucosal-disaccharidases-from-human-intestine
#3
Mahdi Amiri, Hassan Y Naim
In this study, we used a brush border membrane (BBM) preparation from human small intestine to analyze the proportion and the activity of major intestinal disaccharidases, including sucrase-isomaltase (SI), maltase-glucoamylase (MGAM) and lactase-phlorizin hydrolase (LPH). SI, MGAM and LPH respectively constituted 8.2%, 2.7% and 1.4% of total BBM protein. The activity of SI and LPH decreased threefold after purification from the brush border membrane, which highlights the effect of membrane microdomains on the functional capacity of these enzymes...
October 10, 2017: Nutrients
https://www.readbyqxmd.com/read/28994402/crystal-structure-of-the-starch-binding-domain-of-glucoamylase-from-aspergillus-niger
#4
Yousuke Suyama, Norifumi Muraki, Masami Kusunoki, Hideo Miyake
Glucoamylases are widely used commercially to produce glucose syrup from starch. The starch-binding domain (SBD) of glucoamylase from Aspergillus niger is a small globular protein containing a disulfide bond. The structure of A. niger SBD has been determined by NMR, but the conformation surrounding the disulfide bond was unclear. Therefore, X-ray crystal structural analysis was used to attempt to clarify the conformation of this region. The SBD was purified from an Escherichia coli-based expression system and crystallized at 293 K...
October 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28965975/metabolic-engineering-of-aspergillus-oryzae-for-efficient-production-of-l-malate-directly-from-corn-starch
#5
Jingjing Liu, Jianghua Li, Hyun-Dong Shin, Guocheng Du, Jian Chen, Long Liu
l-Malate, an important chemical building block, has been widely applied in the food, pharmaceutical, and bio-based materials industries. In previous work, we engineered Aspergillus oryzae by rewiring the reductive tricarboxylic acid pathway to produce l-malate from glucose. To decrease the production cost, here, we further engineered A. oryzae to efficiently produce l-malate directly from corn starch with simultaneous liquefaction-saccharification and fermentation. First, a promoter PN5 was constructed by quintuple tandem of the 97-bp fragment containing the cis-element of the glucoamylase gene promoter (PglaA), and with the promoter PN5, the transcriptional level of glaA gene increased by 25-45%...
September 28, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28951303/purification-and-biochemical-characterization-of-a-novel-mesophilic-glucoamylase-from-aspergillus-tritici-wz99
#6
Liang Xian, Jia-Xun Feng
Glucoamylase, cleaving the nonreducing end of starch releasing glucose, is an important enzyme in starch processing. The optimal temperature for industrial glucoamylase activity is 60-70°C, which is not compatible with the optimal growth temperature for Saccharomyces cerevisiae. In this study, 26 fungal strains producing amylolytic activities that were more active at 30°C than at 60°C were isolated from 151 environmental samples. Fungal strain WZ99, produced extracellular amylolytic activities with the lowest optimal temperature at 40°C, was identified as Aspergillus tritici by analysis of morphological and molecular data...
September 23, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28941890/comparison-study-of-the-volatile-profiles-and-microbial-communities-of-wuyi-qu-and-gutian-qu-two-major-types-of-traditional-fermentation-starters-of-hong-qu-glutinous-rice-wine
#7
Zhibin Liu, Zhiyao Wang, Xucong Lv, Xiaoping Zhu, Liling Chen, Li Ni
Hong Qu, which mainly contains Monascus sp. and other microorganisms, as well as numerous microbial metabolites, is used as the fermentation starter of Hong Qu glutinous rice wine, a traditional alcoholic beverage. Two widely-used types of Hong Qu, namely Wuyi Qu (WYQ) and Gutian Qu (GTQ), were thoroughly compared for their fermentation properties, volatile profiles, and microbiota structures in this study. Significantly higher color value, glucoamylase and α-amylase activities were discovered in WYQ. And substantial variation in volatile components and microbial communities were also observed between them...
February 2018: Food Microbiology
https://www.readbyqxmd.com/read/28917890/ultrasound-assisted-enzymatic-hydrolysis-of-starch-catalyzed-by-glucoamylase-investigation-on-starch-properties-and-degradation-kinetics
#8
Danli Wang, Xiaobin Ma, Lufeng Yan, Thunthacha Chantapakul, Wenjun Wang, Tian Ding, Xingqan Ye, Donghong Liu
The present work investigates the synergistic impact of glucoamylase and ultrasound on starch hydrolysis. The extent of starch hydrolysis at different reaction parameters (ultrasonic intensity, temperature, reaction time) was analyzed. The hydrolysis extent increased with the reaction time and reached a maximum value under ultrasonic intensity of 7.20W/mL at 10min. Ultrasound did not alter the optimum enzymatic temperature but speeded up the thermal inactivation of glucoamylase. The evaluation of enzymatic kinetics and starch degradation kinetics indicated a promotion of the reaction rate and enzyme-substrate affinity...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28880327/liposome-encoded-magnetic-beads-initiated-by-padlock-exponential-rolling-circle-amplification-for-portable-and-accurate-quantification-of-micrornas
#9
Qingwang Xue, Yancong Kong, Huaisheng Wang, Wei Jiang
Development of reliable and affordable quantitative methods for miRNAs with high specificity and sensitivity is a central challenge to make miRNA testing a routine part of medical care with respect to cancer. Herein, we propose a strategy for glucoamylase-encapsulated liposome-encoded magnetic beads initiated by padlock exponential rolling circle amplification (P-ERCA) for portable and accurate quantification of miRNA by using a glucometer readout.
September 28, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28875706/dietary-flavonoids-and-acarbose-synergistically-inhibit-%C3%AE-glucosidase-and-lower-postprandial-blood-glucose
#10
Bo-Wei Zhang, Xia Li, Wen-Long Sun, Yan Xing, Zhi-Long Xiu, Chun-Lin Zhuang, Yue-Sheng Dong
The inhibition of porcine pancreatic α-amylase and mammalian α-glucosidase by 16 individual flavonoids was determined. The IC50 values for baicalein, (+)-catechin, quercetin, and luteolin were 74.1 ± 5.6, 175.1 ± 9.1, 281.2 ± 19.2, and 339.4 ± 16.3 μM, respectively, against α-glucosidase. The IC50 values for apigenin and baicalein were 146.8 ± 7.1 and 446.4 ± 23.9 μM, respectively, against α-amylase. The combination of baicalein, quercetin, or luteolin with acarbose showed synergistic inhibition, and the combination of (+)-catechin with acarbose showed antagonistic inhibition of α-glucosidase...
September 15, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28861662/extracellular-expression-in-aspergillus-niger-of-an-antibody-fused-to-leishmania-sp-antigens
#11
Denis Magaña-Ortíz, Francisco Fernández, Achim M Loske, Miguel A Gómez-Lim
Nucleoside hydrolase and sterol 24-c-methyltransferase, two antigenic proteins of Leishmania sp., were expressed in Aspergillus niger. Genetic transformation of conidia was achieved using underwater shock waves. scFv antibody addressed to DEC205, a receptor of dendritic cells, was fused to two proteins of Leishmania sp. Receptor 205 has a relevant role in the immune system in mammals; it can modulate T cell response to different antigens. Extracellular expression strategy of recombinant antibody was achieved using a fragment of native glucoamylase A (514 aa) as a carrier...
August 31, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28780468/design-and-synthesis-of-2-6-di-substituted-phenyl-thiazolo-3-2-b-1-2-4-triazoles-as-%C3%AE-glucosidase-and-%C3%AE-amylase-inhibitors-co-relative-pharmacokinetics-and-3d-qsar-and-risk-analysis
#12
Pervaiz Ali Channar, Aamer Saeed, Fayaz Ali Larik, Sajid Rashid, Qaiser Iqbal, Maryam Rozi, Saima Younis, Jamaluddin Mahar
Ten fused heterocyclic derivatives bearing the 2,6-di(subsituted phenyl)thiazolo[3,2-b]-1,2,4-triazoles as central rings were synthesized and structures of the compounds were established by analytical and spectral data using FTIR, EI-MS, (1)H NMR and (13)C NMR techniques. In vitro inhibitory activities of synthesized compounds on α-amylase, α-glucosidase and α-burylcholinesterase (α-BuChE) were evaluated using a purified enzyme assays. Compound 5c demonstrated strong and selective α-amylase inhibitory activity (IC50=1...
October 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28747585/amylolytic-fungi-in-starter-cakes-for-rice-beer-production
#13
Arup Jyoti Das, Tatsuro Miyaji, Sankar Chandra Deka
Two types of starter cakes, viz. amou and perok-kushi, used in the production of rice beer in Assam, India, by the Bodo and Deori communities, respectively, were used for the isolation of amylolytic fungi. Based on the sequencing of their internal transcribed spacer (ITS) regions the fungi were identified as Amylomyces rouxii and Rhizopus oryzae, and given the strain names TU460 and TU465, respectively. Both the strains showed the ability to degrade and saccharify starch. The glucoamylase activity was considerably high in A...
July 27, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28743272/establishment-of-neurospora-crassa-as-a-host-for-heterologous-protein-production-using-a-human-antibody-fragment-as-a-model-product
#14
David Havlik, Ulrike Brandt, Kathrin Bohle, André Fleißner
BACKGROUND: Filamentous fungi are commonly used as production hosts for bulk enzymes in biotechnological applications. Their robust and quick growth combined with their ability to secrete large amounts of protein directly into the culture medium makes fungi appealing organisms for the generation of novel production systems. The red bread mold Neurospora crassa has long been established as a model system in basic research. It can be very easily genetically manipulated and a wealth of molecular tools and mutants are available...
July 25, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28727742/physical-structure-and-absorption-properties-of-tailor-made-porous-starch-granules-produced-by-selected-amylolytic-enzymes
#15
Yi-Seul Jung, Byung-Hoo Lee, Sang-Ho Yoo
Porous starch granules (PSGs) with various pores and cavity sizes were prepared by amylolysis enzymes. The greatest hydrolysis rate on corn starch granule was observed with α-amylase, followed by gluco- and β-amylases. Temperature increase enhanced glucoamylase reaction rate more drastically than other enzyme treatments. Final hydrolysis level with glucoamylase reached to 66.9%, close to 67.5% of α-amylolysis. The α-amylase-treated PSGs displayed the greatest pore size and ratio of cavity-to-granule diameters...
2017: PloS One
https://www.readbyqxmd.com/read/28726400/quinoliniumolate-and-2h-1-2-3-triazole-derivatives-from-the-stems-of-paramignya-trimera-and-their-%C3%AE-glucosidase-inhibitory-activities-in-vitro-and-in-silico-studies
#16
Nhan T Nguyen, Phu H Dang, Ngoc X T Vu, Tho H Le, Mai T T Nguyen
From a CHCl3-soluble extract of the stems of Paramignya trimera, two new alkaloids, (E)-2-(prop-1-enyl)-N-methylquinolinium-4-olate (1) and (R)-2-ethylhexyl 2H-1,2,3-triazole-4-carboxylate (2), were isolated. Their structures were elucidated based on the spectroscopic data interpretation. Compound 2 possesses α-glucosidase inhibitory activity, with an IC50 value of 137.9 μM. Molecular docking studies of 1 and 2 with human maltase-glucoamylase (MGAM) were performed for the first time; thus, the 2,3-diH(+)-1H-1,2,3-triazolium cation (2i) showed good interactions with both MGAM-N (2QMJ) and -C (3TOP) terminal subunits...
July 28, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28724263/mutagenesis-and-characterization-of-a-bacillus-amyloliquefaciens-strain-for-cinnamomum-camphora-seed-kernel-oil-extraction-by-aqueous-enzymatic-method
#17
Cheng Zeng, Rongbin Zhao, Maomao Ma, Zheling Zeng, Deming Gong
The purpose of the present study was to increase the proteinase activity of the strain NCU116 by combining ultraviolet irradiation and N-methyl-N'-nitro-N-nitroso guanidine treatment, in order to enhance the efficiency of Cinnamomum camphora seed kernel oil (CCSKO) extraction by aqueous enzymatic method (AEM). The mutated strain, designated as NCU116-1, was screened out by the ratio of hydrolytic zone diameter to colony diameter on skim milk plate. The proteinase activity (9116.1 U/ml) of NCU116-1 was increased by 31...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28660456/sorghum-husk-biomass-as-a-potential-substrate-for-production-of-cellulolytic-and-xylanolytic-enzymes-by-nocardiopsis-sp-knu
#18
Siddheshwar D Kshirsagar, Bhumika N Bhalkar, Pankajkumar R Waghmare, Ganesh D Saratale, Rijuta G Saratale, Sanjay P Govindwar
Nocardiopsis sp. KNU was found to degrade various lignocellulosic waste materials, namely, sorghum husk, sugarcane tops and leaves, wheat straw, and rice husk very efficiently. The strain was found to produce high amounts of cellulase and hemicellulase. Augmentation of cotton seed cake as an organic nitrogen source revealed inductions in activities of endoglucanase, glucoamylase, and xylanase up to 70.03, 447.89, and 275.10 U/ml, respectively. Nonionic surfactant Tween-80 addition was found to enhance the activity of endoglucanase enzyme...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28637248/expression-and-comparison-of-codon-optimised-aspergillus-tubingensis-amylase-variants-in-saccharomyces-cerevisiae
#19
Rosemary A Cripwell, Shaunita H Rose, Willem H van Zyl
The expression of codon optimised genes is a popular genetic engineering approach for the production of industrially relevant proteins. This study investigates and compares the expression of codon optimised and codon adapted amylase variants. The Aspergillus tubingensis raw starch hydrolysing α-amylase (amyA) and glucoamylase (glaA) encoding genes were redesigned using synonymous codons and expressed in Saccharomyces cerevisiae Y294. Codon optimisation to favour S. cerevisiae codon bias resulted in a decrease in extracellular enzyme activity of 72% (30...
June 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28568243/rethinking-the-starch-digestion-hypothesis-for-amy1-copy-number-variation-in-humans
#20
EDITORIAL
Catalina I Fernández, Andrea S Wiley
Alpha-amylase exists across taxonomic kingdoms with a deep evolutionary history of gene duplications that resulted in several α-amylase paralogs. Copy number variation (CNV) in the salivary α-amylase gene (AMY1) exists in many taxa, but among primates, humans appear to have higher average AMY1 copies than nonhuman primates. Additionally, AMY1 CNV in humans has been associated with starch content of diets, and one known function of α-amylase is its involvement in starch digestion. Thus high AMY1 CNV is considered to result from selection favoring more efficient starch digestion in the Homo lineage...
August 2017: American Journal of Physical Anthropology
keyword
keyword
46099
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"