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Israel Sánchez-Moreno, Carmen Monsalve-Hernando, Ana Godino, Luis Illa, María Jesús Gaspar, Guillermo Manuel Muñoz, Ana Díaz, José Luis Martín, Eduardo García-Junceda, Alfonso Fernández-Mayoralas, Carmen Hermida
Hypolactasia, or intestinal lactase deficiency, affects more than half of the world population. Currently, xylose quantification in urine after gaxilose oral administration for the noninvasive diagnosis of hypolactasia is performed with the hand-operated nonautomatable phloroglucinol reaction. This work demonstrates that a new enzymatic xylose quantification method, based on the activity of xylose dehydrogenase from Caulobacter crescentus , represents an excellent alternative to the manual phloroglucinol reaction...
2017: BioMed Research International
José Luis Domínguez Jiménez, Antonio Fernández Suárez, Aurora Úrsula Muñoz Colmenero, Daniel Fatela Cantillo, Iratxe López Pelayo
BACKGROUND & AIMS: There is no consensus on the most accurate method to diagnose primary hypolactasia. We aimed to compare the diagnostic accuracy of the new gaxilose test with 2 traditional tests (lactose tolerance test and clinical criteria) for the diagnosis of primary hypolactasia using the C/T-13910 polymorphism as a reference standard. METHODS: Patients with a clinical suspicion of lactose intolerance were subjected to gaxilose tests, shortened lactose tolerance tests, and symptom questionnaires before and after overload with 50 g lactose and after a lactose-free diet...
April 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
Carmen Hermida, Oscar H Martínez-Costa, Guillermo Corrales, Cristina Teruel, Valentina Sánchez, José J Sánchez, Dolores Sarrión, María J Ariza, Rosa Codoceo, Idoya Calvo, Alfonso Fernández-Mayoralas, Juan J Aragón
BACKGROUND: The phloroglucinol assay is the current method for d-xylose determination in urine/plasma/serum. However, its sensitivity is limited when low amounts of d-xylose are to be measured, such as in the noninvasive evaluation of intestinal lactase with 4-galactosylxylose (gaxilose). An improved assay was therefore needed. METHODS: We developed and validated a modified version of the phloroglucinol-based assay for quantification of d-xylose in urine/serum samples...
November 2014: Journal of Clinical Laboratory Analysis
Juan J Aragón, Carmen Hermida, Oscar H Martínez-Costa, Valentina Sánchez, Igor Martín, José J Sánchez, Rosa Codoceo, José M Cano, Ana Cano, Laura Crespo, Yolanda Torres, Francisco J García, Alfonso Fernández-Mayoralas, Jesús Solera, Pilar Martínez
GOALS AND BACKGROUND: Hypolactasia affects over half of the world population. Diagnosis remains problematic as currently available tests, such as the hydrogen breath test, have low reliability and lactose intolerance symptoms are unspecific. We evaluated the diagnostic performance and safety of a new noninvasive diagnostic test based on urine or serum measurement of D-xylose after lactase cleavage of orally administered 4-galactosylxylose (gaxilose). STUDY: In a multicentre, open-label, nonrandomized, phase IIb-III study, consecutive patients with symptoms suggestive of lactose intolerance sequentially underwent intestinal biopsy for direct measurement of lactase activity (reference standard), hydrogen breath test, and blood glucose test after lactose challenge, 4- and 5-hour urine-based gaxilose test, and blood-based gaxilose test...
January 2014: Journal of Clinical Gastroenterology
Carmen Hermida, Pedro Guerra, Oscar H Martínez-Costa, Valentina Sánchez, José J Sánchez, Jesús Solera, Alfonso Fernández-Mayoralas, Rosa Codoceo, Jesús Frías, Juan J Aragón
GOALS AND BACKGROUND: Hypolactasia is widespread, yet reliable diagnostic tests are lacking. A new test based on oral administration of 4-galactosylxylose (gaxilose) and urine or serum measurement of D-xylose after cleavage by intestinal lactase is under clinical development. We investigated the optimal dose of gaxilose and calculate cutoff values of D-xylose for that dose. STUDY: In the randomized, dose-finding, phase I study, urine and serum pharmacokinetics of D-xylose were determined after oral administration of 6 ascending doses of gaxilose (and placebo) to 12 healthy adult volunteers...
July 2013: Journal of Clinical Gastroenterology
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