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antimicrobial coating

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https://www.readbyqxmd.com/read/28537751/using-neutron-reflectometry-to-characterize-antimicrobial-protein-surface-coatings
#1
Peter W Akers, Andrew J Dingley, Simon Swift, Andrew R J Nelson, Julie Martin, Duncan James McGillivray
Understanding the interaction of adsorbed or covalently immobilized proteins with solid substrates at the molecular level guides the successful design of functionalized surfaces used in biomedical applications. In this report, neutron reflectometry (NR) was used to characterize the structure of surface-attached antimicrobial protein films, with antimicrobial activity assessed using an adaption of the Japanese Industrial Standard Test JIS Z 2801. NR allowed parameters influencing bioactivity to be measured at nanometer resolution and for conclusions about structural characteristics relating to bioactivity to be drawn...
May 24, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28534703/chlorhexidine-and-silver-sulfadiazine-coating-on-central-venous-catheters-is-not-sufficient-for-protection-against-catheter-related-infection-simulation-based-laboratory-research-with-clinical-validation
#2
Yoon Ji Choi, Jae Kwan Lim, Jeong Jun Park, Hyub Huh, Dong-Joo Kim, Chang-Hoon Gong, Seung Zhoo Yoon
Objective The efficacy of chlorhexidine- and silver sulfadiazine-coated central venous catheters (CSS-CVC) against catheter-related infection remains controversial. We hypothesized that the loss of silver nanoparticles may reduce the antibacterial efficacy of CSS-CVCs and that this loss could be due to the frictional force between the surface of the CVC and the bloodstream. The objective of this study was to investigate whether the antimicrobial effect of CSS-CVCs decreases with increasing exposure time in a bloodstream model and quantitatively assay the antimicrobial effect of CSS-CVCs compared with polyurethane and antiseptic-impregnated CVCs...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28532068/synthetic-peptides-derived-from-salivary-proteins-and-the-control-of-surface-charge-densities-of-dental-surfaces-improve-the-inhibition-of-dental-calculus-formation
#3
Bernd Grohe
Peptides descended from the salivary proteins statherin and histatin were recently identified in saliva and the acquired enamel pellicle (AEP), a proteomic layer coated on enamel. In particular, the statherin phosphopeptide DpSpSEEKFLR (DSS) was found to adsorb to enamel-like hydroxyapatite and inhibit plaque-related crystal formation. To determine the mechanism of these processes, we studied peptide-crystal interactions based on the sequences DSS and RKFHEKHHSHRGYR (RKF). The latter is a basic histatin sequence showing antimicrobial effects...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28532029/surfactin-loaded-polyvinyl-alcohol-pva-nanofibers-alters-adhesion-of-listeria-monocytogenes-to-polystyrene
#4
J J Ahire, D D Robertson, A J van Reenen, L M T Dicks
Surfactin-loaded polyvinyl alcohol (PVA) nanofibers were spun using gravity electrospinning. Scanning electron microscopy (SEM) images showed that nanofibers spun with surfactin are free from bead formation and uniform in diameter. The average nanofiber diameters were decreased (273±39nm, 259±39nm and 217±33nm) with increasing levels of surfactin (0.5, 1.0 and 1.5%, w/v) into PVA (10%, w/v). The 10% (w/v) PVA had average fiber diameter of 303±33nm. Atomic force microscopy (AFM) analysis showed that fibers spun with surfactin are not smooth as PVA fibers...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531982/study-of-the-keratin-based-therapeutic-dermal-patches-for-the-delivery-of-bioactive-molecules-for-wound-treatment
#5
Kush Kumar Nayak, Pratima Gupta
In the present study, dressing materials were fabricated in the form of dermal patches. The keratin based biocompatible materials, namely Keratin/Alginate, Keratin/Agar, and Keratin/Gellan were developed as therapeutic dermal patches. The patches were coated with green synthesized silver nanoparticles to prevent the microbial infection and were found to be antimicrobial against pathogens. Papain was used in the patches to acquire the enzymatic cell debridement and its activity was measured by reduction rate of benzoyl-l-arginine ethyl ester...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531875/elaboration-of-antibacterial-plastic-surfaces-by-a-combination-of-antiadhesive-and-biocidal-coatings-of-natural-products
#6
Jean-Baptiste Paris, Damien Seyer, Thierry Jouenne, Pascal Thébault
Antibacterial polyolefins surfaces, combining biocidal and antiadhesive properties, were elaborated by a covalent grafting of antimicrobial peptides (AMPs), able to kill adherent bacteria, on a pre-immobilized hyaluronic acid (HA) layer, able to repel the micro-organisms. Different HA activation rate for its immobilization, were used to change HA layer morphology and number of residual free carboxylic acid functions for AMPs grafting. Based on adhesion tests on Staphylococcus epidermidis and microscopy fluorescent observations, the presence of the two combined properties was shown to be depended on the HA activation rate...
May 13, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28530300/physicochemical-and-antimicrobial-properties-of-%C3%AE%C2%B5-polylysine-carboxymethyl-chitosan-polyelectrolyte-complexes-and-their-effect-against-spoilage-microorganisms-in-raw-pork
#7
Liang Guo, Yuecheng Meng, Sheng Fang
This study investigated the properties of polyelectrolyte complexes (PECs) fabricated by using ε-polylysine (EPL) and N,O-carboxymethyl chitosan (NOCC) and their potential applications in raw pork. The phase behaviors of PECs in aqueous solutions are characterized by the ζ-potential, mean radius and turbidimetric measurements. Stable colloidal and soluble PEC systems can be fabricated by carefully adjusting the mass ratio of NOCC to EPL. The minimum inhibitory concentration (MIC) assay showed that the colloidal and soluble PECs could maintain the antimicrobial activity of EPL in a laboratory medium...
May 22, 2017: Food & Function
https://www.readbyqxmd.com/read/28529865/hyaluronic-acid-and-its-composites-as-a-local-antimicrobial-antiadhesive-barrier
#8
REVIEW
C L Romanò, E De Vecchi, M Bortolin, I Morelli, L Drago
Living in biofilms is probably the most common condition for bacteria and fungi and biofilm-related infections account for the majority of bacterial infectious diseases worldwide. Among others biofilm-related infections, those associated with implanted biomaterials have an enormous and still largely underestimated impact in orthopaedics and trauma, cardio-surgery and several other surgical disciplines. Given the limited efficacy of existing antibiotics in the prevention and treatment of bacterial biofilms, new strategies are needed to protect implants and host tissues, overcoming the striking ability of the microorganisms to adhere on different surfaces and to immediately protect themselves by forming the biofilm matrix...
2017: J Bone Jt Infect
https://www.readbyqxmd.com/read/28527354/incorporation-of-silver-nanoparticles-into-magnetron-sputtered-calcium-phosphate-layers-on-titanium-as-an-antibacterial-coating
#9
Maria A Surmeneva, Anna A Sharonova, Svitlana Chernousova, Oleg Prymak, Kateryna Loza, Mikhail S Tkachev, Ivan A Shulepov, Matthias Epple, Roman A Surmenev
A three-layer system of nanocrystalline hydroxyapatite (first layer; 1000nm thick), silver nanoparticles (second layer; 1.5μg Ag cm(-2)) and calcium phosphate (third layer, either 150 or 1000nm thick) on titanium was prepared by a combination of electrophoretic deposition of silver nanoparticles and the deposition of calcium phosphate by radio frequency magnetron sputtering. Scanning electron microscopy showed that the silver nanoparticles were evenly distributed over the surface. The adhesion of multilayered coating on the substrate was evaluated using the scratch test method...
May 9, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28521994/double-coated-enrofloxacin-microparticles-with-chitosan-and-alginate-preparation-characterization-and-taste-masking-effect-study
#10
Mengxi Liu, Daiping Yin, Hualin Fu, Fengying Deng, Guangneng Peng, Gang Shu, Zhixiang Yuan, Fei Shi, Juchun Lin, Ling Zhao, Lizi Yin, Guoqing Fan
Enrofloxacin (ENRO) is widely used as an antimicrobial drug for treatment of uncomplicated and complicated infections in veterinary medicine. Its bitter taste limits its clinical applications in veterinary. To mask the bitter taste of this drug, double-coated taste-masking microparticles of ENRO (DTME) were prepared through stearic acid solid dispersion and chitosan-alginate microparticle coating technologies. The taste-masking effect was evaluated by pig feeding experiment. Results showed that DTME exhibited a spherical-like shape (170490μm)...
August 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28512650/effect-of-antimicrobial-photodynamic-therapy-%C3%A2-and-laser-alone-as-adjunct-to-mechanical-debridement-in-the-management-of-halitosis-a-systematic-review
#11
Sergio Varela Kellesarian, Vanessa Ros Malignaggi, Abdulaziz A Al-Kheraif, Mansour Al-Askar, Michael Yunker, Fawad Javed
OBJECTIVE: The aim of the present study was to assess the efficacy of laser therapy (LT) and antimicrobial photodynamic therapy (aPDT) as adjunct to mechanical debridement (MD) on the management of halitosis. DATA SOURCES: In order to address the focused question "Is MD with adjunct LT and/or aPDT more effective in the management of halitosis compared with MD alone?" an electronic search without time or language restrictions was conducted up to January 2017 in indexed databases using the combination of different key words including photochemotherapy, lasers, light, photodynamic therapy, halitosis, and bad breath...
May 16, 2017: Quintessence International
https://www.readbyqxmd.com/read/28511708/enzyme-mediated-formulation-of-stable-elliptical-silver-nanoparticles-tested-against-clinical-pathogens-and-mdr-bacteria-and-development-of-antimicrobial-surgical-thread
#12
Rupak Thapa, Chintan Bhagat, Pragya Shrestha, Suvash Awal, Pravin Dudhagara
BACKGROUND: Silver nanoparticles (AgNPs) are believed to be emerging tool against various infectious diseases including multi-drug resistant (MDR) bacteria. In the present study, in vitro synthesis of AgNPs was optimized using 1:50 ratio of macerozyme (25 μg/μl) and 1 mM AgNO3 incubated at 80 °C for 8 h. AgNPs were characterized by UV-Visible spectroscopy, dynamic light scattering (DLS), scanning electron microscopy, energy-dispersive X-ray spectroscopy, transmission electron microscopy (TEM) and X-ray diffraction (XRD)...
May 16, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28502123/novel-method-for-the-depletion-of-cariogenic-bacteria-using-dextranomer-microspheres
#13
Lauren Mashburn-Warren, Jennifer S Downey, Steven D Goodman
Streptococcus mutans is recognized as one of the key contributors to the dysbiotic state that results in dental caries. While existing treatment strategies reduce the incidence of tooth decay, they also eliminate both the cariogenic and beneficial microbes. Here we introduce a novel treatment alternative using Sephadex, cross-linked dextranomer microspheres (DMs), typically used for gel filtration chromatography. In addition DM beads can be used for affinity purification of glucosyltransferases (GTFs) from S...
May 14, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/28499217/adhesive-coatings-based-on-melanin-like-nanoparticles-for-surgical-membranes
#14
Francesca Scognamiglio, Andrea Travan, Gianluca Turco, Massimiliano Borgogna, Eleonora Marsich, Mattia Pasqua, Sergio Paoletti, Ivan Donati
Adhesive coatings for implantable biomaterials can be designed to prevent material displacement from the site of implant. In this paper, a strategy based on the use of melanin-like nanoparticles (MNPs) for the development of adhesive coatings for polysaccharidic membranes was devised. MNPs were synthesized in vitro and characterized in terms of dimensions and surface potential, as a function of pH and ionic strength. The in vitro biocompatibility of MNPs was investigated on fibroblast cells, while the antimicrobial properties of MNPs in suspension were evaluated on E...
April 29, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28494101/stability-enhancement-of-ocimum-sanctum-linn-essential-oils-using-stearic-acid-in-aluminum-carboxymethyl-cellulose-film-coated-gelatin-microcapsules
#15
Pakamon Chitprasert, Narisara Ngamekaue
Holy basil essential oils (HBEO) can be used in many food applications due to antioxidant and antimicrobial attributes, but they are susceptible to degradation upon storage. Therefore, a protective system is required to extend their shelf life. HBEO was microencapsulated by coacervation using gelatin and the microcapsules were subsequently coated with stearic acid (1%, 2%, and 3%) in carboxymethyl cellulose emulsions. The results showed that HBEO contents decreased with increasing stearic acid concentrations from 76% to 59%...
May 11, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28488039/development-of-antimicrobial-coating-by-later-by-layer-dip-coating-of-chlorhexidine-loaded-micelles
#16
Supreeda Tambunlertchai, Siriwan Srisang, Norased Nasongkla
Layer-by-layer (LbL) dip coating, accompanying with the use of micelle structure, allows hydrophobic molecules to be coated on medical devices' surface via hydrogen bonding interaction. In addition, micelle structure also allows control release of encapsulated compound. In this research, we investigated methods to coat and maximize the amount of chlorhexidine (CHX) on silicone surface through LbL dip coating method utilizing hydrogen bonding interaction between PEG on micelle corona and PAA. The number of coated cycles was varied in the process and 90 coating cycles provided the maximum amount of CHX loaded onto the surface...
June 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28483964/towards-elimination-of-pin-tract-infections-novel-antibacterial-coating-on-orthopaedic-wires
#17
Dmitry Gil, Sergey Shuvaev, Anastasia Frank-Kamenetskii, Vladimir Reukov, Christopher Gross, Alexey Vertegel
Novel approaches to prevention of microbial infections after insertion of orthopedic external fixators are in great demand because of extremely high incidence rate of such infections, which can reach up to 100% for longer implant residence times. Monolaurin is an antimicrobial agent with known safety record, broadly used in food and cosmetic industries; however, its use in antimicrobial coatings of medical devices has not been studied in much detail. Here, we report the use of monolaurin as an antibacterial coating on external fixators for the first time...
May 8, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28482553/iodine-loaded-metal-organic-framework-as-growth-triggered-antimicrobial-agent
#18
Ai-Nhan Au-Duong, Cheng-Kang Lee
Zeolitic imidazolate framework-8 (ZIF-8) is one of easily available metal organic frameworks because of its facile preparation via mixing aqueous solutions of zinc nitrate and 2-methylimidazole. It turned into a very effective pH-responsive bactericide after loading with iodine. Approximately, 0.9g of iodine could be readily loaded into one gram of ZIF-8 from iodine dissolved n-heptane solution. Both Gram-negative Escherichia coli and Gram-positive Staphylococcus epidermidis and Staphylococcus aureus could be very effectively killed by iodine loaded ZIF-8 (ZIF-8@I) at pH6...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28475440/the-effect-of-negative-pressure-wound-therapy-with-antibacterial-dressings-or-antiseptics-on-an-in-vitro-wound-model
#19
J Matiasek, K J Domig, G Djedovic, R Babeluk, O Assadian
OBJECTIVE: The aim of this study was to investigate the bacterial bioburden in experimental in vitro wounds during the application of conventional negative pressure wound therapy (NPWT), with and without antimicrobial dressings (polyhexanide, silver), against NPWT instillation of octenidine. METHOD: Experimental wounds produced in an in vitro porcine wound model were homogenously contaminated with bacterial suspension and treated with NPWT and different options...
May 2, 2017: Journal of Wound Care
https://www.readbyqxmd.com/read/28474012/covalent-attachment-of-daptomycin-to-ti6al4v-alloy-surfaces-by-a-thioether-linkage-to-inhibit-colonization-by-staphylococcus-aureus
#20
Chang-Po Chen, Rui-Yan Jing, Eric Wickstrom
Infections are a devastating complication of titanium alloy orthopedic implants. Current therapies include antibiotic-impregnated bone cement and antibiotic-containing coatings. Daptomycin (DAP) (1) is a novel peptide antibiotic that penetrates the cell membranes of Gram-positive bacteria. Few DAP-resistant strains have appeared so far. We hypothesized that when DAP covalently bonded via a flexible, hydrophilic spacer it could prevent bacterial colonization of titanium alloy surfaces. We designed and synthesized a series of DAP conjugates for bonding to the surface of Ti6Al4V foils through tetra(ethylene glycol) spacers via thioether linkages...
April 30, 2017: ACS Omega
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