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Regeneration kidney

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https://www.readbyqxmd.com/read/28922990/streamlined-system-for-conducting-in-vitro-studies-using-decellularized-kidney-scaffolds
#1
Andrew Reyes Padalhin, Chanmi Park, Byong-Taek Lee
Kidney regeneration is a complex process that can only be studied in vitro at a limited capacity due to the inherent structural and functional complexity of its tissues. Thus, a suitable platform for conducting cellular response and development should be established from decellularized tissues with intact microarchitecture. In this study, a modular streamlined system was developed to allow manageable handling and set-up of in vitro studies using decellularized rat kidneys. The system is composed of commercially available parts that can be reused, interchanged, and re-configured based on the desired experimental stage and process...
September 19, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28905451/gene-edited-human-kidney-organoids-reveal-mechanisms-of-disease-in-podocyte-development
#2
Yong Kyun Kim, Ido Refaeli, Craig R Brooks, Peifeng Jing, Ramila E Gulieva, Michael R Hughes, Nelly M Cruz, Yannan Liu, Angela J Churchill, Yuliang Wang, Hongxia Fu, Jeffrey W Pippin, Lih Y Lin, Stuart J Shankland, A Wayne Vogl, Kelly M McNagny, Benjamin S Freedman
A critical event during kidney organogenesis is the differentiation of podocytes, specialized epithelial cells that filter blood plasma to form urine. Podocytes derived from human pluripotent stem cells (hPSC-podocytes) have recently been generated in nephron-like kidney organoids, but the developmental stage of these cells and their capacity to reveal disease mechanisms remains unclear. Here we show that hPSC-podocytes phenocopy mammalian podocytes at the capillary loop stage (CLS), recapitulating key features of ultrastructure, gene expression, and mutant phenotype...
September 14, 2017: Stem Cells
https://www.readbyqxmd.com/read/28887969/viability-of-hek-293-cells-on-poly-%C3%AE-hydroxybutyrate-phb-biosynthesized-from-a-mutant-azotobacter-vinelandii-strain-cast-film-and-electrospun-scaffolds
#3
Angel Romo-Uribe, Angelica Meneses-Acosta, Maraolina Domínguez-Díaz
Sterilization, cytotoxicity and cell viability are essential properties defining a material for medical applications and these characteristics were investigated for poly(β-hydroxybutyrate) (PHB) of 230kDa obtained by bacterial synthesis from a mutant strain of Azotobacter vinelandii. Cell viability was investigated for two types of PHB scaffolds, solution cast films and non-woven electrospun fibrous membranes, and the efficiency was compared against a culture dish. The biosynthesized PHB was sterilized by ultraviolet radiation and autoclave, it was found that the thermal properties and intrinsic viscosity remained unchanged indicating that the sterilization methods did not degrade the polymer...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28869686/concise-reviews-kidney-generation-with-human-pluripotent-stem-cells
#4
REVIEW
Ryuji Morizane, Tomoya Miyoshi, Joseph V Bonventre
Chronic kidney disease (CKD) is a world-wide healthcare problem, resulting in increased cardiovascular mortality and often leading to end-stage kidney disease (ESKD) where patients require kidney replacement therapies such as hemodialysis or kidney transplantation. Loss of functional nephrons contributes to the progression of CKD, which can be attenuated but not reversed due to inability to generate new nephrons in human adult kidneys. Human pluripotent stem cells (hPSCs), by virtue of their unlimited self-renewal and ability to differentiate into cells of all three embryonic germ layers, are attractive sources for kidney regenerative therapies...
September 4, 2017: Stem Cells
https://www.readbyqxmd.com/read/28858213/recreational-exposure-during-algal-bloom-in-carrasco-beach-uruguay-a-liver-failure-case-report
#5
Flavia Vidal, Daniela Sedan, Daniel D'Agostino, María Lorena Cavalieri, Eduardo Mullen, María Macarena Parot Varela, Cintia Flores, Josep Caixach, Dario Andrinolo
In January 2015, a 20-month-old child and her family took part in recreational activities at Carrasco and Malvín beaches (Montevideo, Uruguay). An intense harmful algae bloom (HAB) was developing along the coast at that time. A few hours after the last recreational exposure episode, the family suffered gastrointestinal symptoms which were self-limited except in the child's case, who was admitted to hospital in Uruguay with diarrhea, vomiting, fatigue, and jaundice. The patient had increased serum levels of liver enzymes and bilirubin and five days later presented acute liver failure...
August 31, 2017: Toxins
https://www.readbyqxmd.com/read/28841424/mechanical-induction-of-dentin-like-differentiation-by-adult-mouse-bone-marrow-stromal-cells-using-compressive-scaffolds
#6
Basma Hashmi, Tadanori Mammoto, James Weaver, Thomas Ferrante, Amanda Jiang, Elisabeth Jiang, Juani Feliz, Donald E Ingber
Tooth formation during embryogenesis is controlled through a complex interplay between mechanical and chemical cues. We have previously shown that physical cell compaction of dental mesenchyme cells during mesenchymal condensation is responsible for triggering odontogenic differentiation during embryogenesis, and that expression of Collagen VI stabilizes this induction. In addition, we have shown that synthetic polymer scaffolds that artificially induce cell compaction can induce embryonic mandible mesenchymal cells to initiate tooth differentiation both in vitro and in vivo...
August 17, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28831294/ameliorative-effect-of-daidzein-on-cisplatin-induced-nephrotoxicity-in-mice-via-modulation-of-inflammation-oxidative-stress-and-cell-death
#7
Hongzhou Meng, Guanghou Fu, Jie Shen, Kezhen Shen, Zhijie Xu, Yiming Wang, Baiye Jin, Hao Pan
Oxidative stress and inflammation are part and parcel of cisplatin-induced nephrotoxicity. The purpose of this work is to study the role of soy isoflavone constituent, daidzein, in cisplatin-induced renal damage. Cisplatin-induced nephrotoxicity was evident by the histological damage in proximal tubular cells and by the increase in serum neutrophil gelatinase-associated lipocalin (NGAL), blood urea nitrogen (BUN), creatinine, and urinary kidney injury molecule-1 (KIM-1). Cisplatin-induced cell death was shown by TUNEL staining and caspase-3/7 activity...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28819717/mitigation-of-acute-aluminum-toxicity-by-sodium-selenite-and-n-acetylcysteine-in-adult-male-rats
#8
Naglaa H Nour-Eldein, El-Sayed A Hassanin, Wael M El-Sayed
The objective of this study is to investigate the toxic effects of aluminum and the potential alleviation of selenite and N-acetylcysteine (NAC) on this toxicity. Acute aluminum toxicity was induced by intraperitoneal (i.p.) injection of AlCl3 (30 mg Al(3+)/kg) for four consecutive days. Al(3+) damaged the synthetic capability and regeneration power of liver cells and induced inflammation. It also damaged the kidney and disturbed the lipid profile enhancing the total cholesterol level and LDL-cholesterol level increasing the risks of atherosclerosis...
August 17, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28811645/inhibin-a-and-decorin-secreted-by-human-adult-renal-stem-progenitor-cells-through-the-tlr2-engagement-induce-renal-tubular-cell-regeneration
#9
Fabio Sallustio, Claudia Curci, Alessandra Aloisi, Chiara Cristina Toma, Elisabetta Marulli, Grazia Serino, Sharon Natasha Cox, Giuseppe De Palma, Alessandra Stasi, Chiara Divella, Rosaria Rinaldi, Francesco Paolo Schena
Acute kidney injury (AKI) is a public health problem worldwide. Several therapeutic strategies have been made to accelerate recovery and improve renal survival. Recent studies have shown that human adult renal progenitor cells (ARPCs) participate in kidney repair processes, and may be used as a possible treatment to promote regeneration in acute kidney injury. Here, we show that human tubular ARPCs (tARPCs) protect physically injured or chemically damaged renal proximal tubular epithelial cells (RPTECs) by preventing cisplatin-induced apoptosis and enhancing proliferation of survived cells...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28795416/the-acute-effect-of-beta-guanidinopropionic-acid-versus-creatine-or-placebo-in-healthy-men-abc-trial-a-randomized-controlled-first-in-human-trial
#10
F A Karamat, D L Horjus, Y C Haan, L van der Woude, M C Schaap, I Oudman, G A van Montfrans, R Nieuwland, G S Salomons, J F Clark, L M Brewster
AIM: Increasing evidence indicates that the ATP-generating enzyme creatine kinase (CK) is involved in hypertension. CK rapidly regenerates ATP from creatine phosphate and ADP. Recently, we showed that beta-guanidinopropionic acid (GPA), a kidney-synthesized creatine analogue and competitive CK inhibitor, reduced blood pressure in spontaneously hypertensive rats. To further develop the substance as a potential blood pressure-lowering agent, we assessed the tolerability of a sub-therapeutic GPA dose in healthy men...
August 10, 2017: British Journal of Clinical Pharmacology
https://www.readbyqxmd.com/read/28774079/synthesis-and-in-vitro-activity-assessment-of-novel-silicon-oxycarbide-based-bioactive-glasses
#11
Isabel Gonzalo-Juan, Rainer Detsch, Sanjay Mathur, Emanuel Ionescu, Aldo R Boccaccini, Ralf Riedel
Novel bioactive glasses based on a Ca- and Mg-modified silicon oxycarbide (SiCaMgOC) were prepared from a polymeric single-source precursor, and their in vitro activity towards hydroxyapatite mineralization was investigated upon incubating the samples in simulated body fluid (SBF) at 37 °C. The as-prepared materials exhibit an outstanding resistance against devitrification processes and maintain their amorphous nature even after exposure to 1300 °C. The X-ray diffraction (XRD) analysis of the SiCaMgOC samples after the SBF test showed characteristic reflections of apatite after only three days, indicating a promising bioactivity...
November 24, 2016: Materials
https://www.readbyqxmd.com/read/28760433/branched-chain-amino-acid-supplementation-in-treatment-of-liver-cirrhosis-updated-views-on-how-to-attenuate-their-harmful-effects-on-cataplerosis-and-ammonia-formation
#12
REVIEW
Milan Holeček
Branched-chain amino acid (BCAA; valine, leucine, and isoleucine) supplementation is common for patients with liver cirrhosis due to decreased levels of BCAA in the blood plasma of these patients, which plays a role in pathogenesis of hepatic encephalopathy and cachexia. The unique pharmacologic properties of BCAA also are a factor for use as supplementation in this population. In the present article, BCAA is shown to provide nitrogen to alpha-ketoglutarate (α-KG) for synthesis of glutamate, which is a substrate for ammonia detoxification to glutamine (GLN) in the brain and muscles...
September 2017: Nutrition
https://www.readbyqxmd.com/read/28736435/extracellular-vesicles-in-renal-disease
#13
REVIEW
Diana Karpman, Anne-Lie Ståhl, Ida Arvidsson
Extracellular vesicles, such as exosomes and microvesicles, are host cell-derived packages of information that allow cell-cell communication and enable cells to rid themselves of unwanted substances. The release and uptake of extracellular vesicles has important physiological functions and may also contribute to the development and propagation of inflammatory, vascular, malignant, infectious and neurodegenerative diseases. This Review describes the different types of extracellular vesicles, how they are detected and the mechanisms by which they communicate with cells and transfer information...
September 2017: Nature Reviews. Nephrology
https://www.readbyqxmd.com/read/28735502/regenerative-medicine-in-kidney-disease-where-we-stand-and-where-to-go
#14
REVIEW
Fernanda T Borges, Nestor Schor
The kidney is a complex organ with more than 20 types of specialized cells that play an important role in maintaining the body's homeostasis. The epithelial tubular cell is formed during embryonic development and has little proliferative capacity under physiological conditions, but after acute injury the kidney does have regenerative capacity. However, after repetitive or severe lesions, it may undergo a maladaptation process that predisposes it to chronic kidney injury. Regenerative medicine includes various repair and regeneration techniques, and these have gained increasing attention in the scientific literature...
July 22, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28727410/exosomes-mediate-epithelium-mesenchyme-crosstalk-in-organ-development
#15
Nan Jiang, Lusai Xiang, Ling He, Guodong Yang, Jinxuan Zheng, Chenglin Wang, Yimei Zhang, Sainan Wang, Yue Zhou, Tzong-Jen Sheu, Jiaqian Wu, Kenian Chen, Paulo G Coelho, Nicky M Tovar, Shin Hye Kim, Mo Chen, Yan-Heng Zhou, Jeremy J Mao
Organ development requires complex signaling by cells in different tissues. Epithelium and mesenchyme interactions are crucial for the development of skin, hair follicles, kidney, lungs, prostate, major glands, and teeth. Despite myriad literature on cell-cell interactions and ligand-receptor binding, the roles of extracellular vesicles in epithelium-mesenchyme interactions during organogenesis are poorly understood. Here, we discovered that ∼100 nm exosomes were secreted by the epithelium and mesenchyme of a developing tooth organ and diffused through the basement membrane...
August 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/28714420/evaluation-of-temporal-changes-in-urine-based-metabolomic-and-kidney-injury-markers-to-detect-compound-induced-acute-kidney-tubular-toxicity-in-beagle-dogs
#16
M P Wagoner, Y Yang, J E McDuffie, M Klapczynski, W Buck, L Cheatham, D Eisinger, F Sace, K M Lynch, M Sonee, J-Y Ma, Y Chen, K Marshall, M Damour, L Stephen, Y P Dragan, J Fikes, S Snook, L B Kinter
Urinary protein biomarkers and metabolomic markers have been leveraged to detect acute drug induced kidney injury (DIKI) in rats; however, the utility of these indicators to enable early detection of DIKI in canine models has not been well documented. Therefore, we evaluated temporal changes in biomarkers and metabolites in urine from male and female beagle dogs. Gentamicin-induced kidney lesions in male dogs were characterized by moderate to severe tubular epithelial cell degeneration/necrosis, epithelial cell regeneration and dilation; and a unique urine-based metabolomic fingerprint...
July 13, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28711779/hyperactive-foxo1-results-in-lack-of-tip-stalk-identity-and-deficient-microvascular-regeneration-during-kidney-injury
#17
Lan T H Dang, Takahide Aburatani, Graham A Marsh, Bryce G Johnson, Stella Alimperti, Christine J Yoon, Angela Huang, Suzanne Szak, Naoki Nakagawa, Ivan Gomez, Shuyu Ren, Sarah K Read, Chris Sparages, Alfred C Aplin, Roberto F Nicosia, Chris Chen, Giovanni Ligresti, Jeremy S Duffield
Loss of the microvascular (MV) network results in tissue ischemia, loss of tissue function, and is a hallmark of chronic diseases. The incorporation of a functional vascular network with that of the host remains a challenge to utilizing engineered tissues in clinically relevant therapies. We showed that vascular-bed-specific endothelial cells (ECs) exhibit differing angiogenic capacities, with kidney microvascular endothelial cells (MVECs) being the most deficient, and sought to explore the underlying mechanism...
October 2017: Biomaterials
https://www.readbyqxmd.com/read/28704129/electromagnetic-information-transfer-through-aqueous-system
#18
Alberto Foletti, Mario Ledda, Maria Grazia Lolli, Settimio Grimaldi, Antonella Lisi
Several beneficial effects of the electromagnetic information transfer through aqueous system (EMITTAS) procedure have previously been reported in vitro. The clinical potential of this procedure has also started to be evaluated. Information flow in biological systems can be investigated through chemical and molecular approaches or by a biophysical approach focused on endogenous electrodynamic activities. Electromagnetic signals are endogenously generated at different levels of the biological organization and, likely, play an active role in synchronizing internal cell function or local/systemic adaptive response...
July 13, 2017: Electromagnetic Biology and Medicine
https://www.readbyqxmd.com/read/28699526/hydrogel-based-cell-therapies-for-kidney-regeneration-current-trends-in-biofabrication-and-in-vivo-repair
#19
Katja Jansen, Carl C L Schuurmans, Jitske Jansen, Rosalinde Masereeuw, Tina Vermonden
Facing the problems of limited renal regeneration capacity and the persistent shortage of donor kidneys, dialysis remains the only treatment option for many end-stage renal disease patients. Unfortunately, dialysis is only a medium-term solution because large and protein-bound uremic solutes are not efficiently cleared from the body and lead to disease progression over time. Current strategies for improved renal replacement therapies (RRTs) range from whole organ engineering to biofabrication of renal assist devices and biological injectables for in vivo regeneration...
July 10, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28698077/examination-of-in-vitro-and-in-vivo-biocompatibility-of-alginate-hyaluronic-acid-microbeads-as-a-promising-method-in-cell-delivery-for-kidney-regeneration
#20
Jhaleh Amirian, Tran Thi Tuong Van, Sang-Ho Bae, Hae-Il Jung, Hwan-Jun Choi, Hyun-Deuk Cho, Byong-Taek Lee
In this study, alginate (ALG) and alginate-hyaluronic acid (ALG-HA) injectable microbeads, with the purpose of delivering stem cells for tissue engineering, were prepared by a spraying method into a CaCl2 solution that shows high porosity for the exchange of nutrition and waste. In addition, the size distribution and surface morphology was investigated using optical and scanning electron microscopy, respectively. The chemical structural properties of the ALG-HA microbeads were examined by Fourier transform infrared spectroscopy...
July 8, 2017: International Journal of Biological Macromolecules
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