Mattia Francesco Maria Gerli, Giuseppe Calà, Max Arran Beesley, Beatrice Sina, Lucinda Tullie, Kylin Yunyan Sun, Francesco Panariello, Federica Michielin, Joseph R Davidson, Francesca Maria Russo, Brendan C Jones, Dani Do Hyang Lee, Savvas Savvidis, Theodoros Xenakis, Ian C Simcock, Anna A Straatman-Iwanowska, Robert A Hirst, Anna L David, Christopher O'Callaghan, Alessandro Olivo, Simon Eaton, Stavros P Loukogeorgakis, Davide Cacchiarelli, Jan Deprest, Vivian S W Li, Giovanni Giuseppe Giobbe, Paolo De Coppi
Isolation of tissue-specific fetal stem cells and derivation of primary organoids is limited to samples obtained from termination of pregnancies, hampering prenatal investigation of fetal development and congenital diseases. Therefore, new patient-specific in vitro models are needed. To this aim, isolation and expansion of fetal stem cells during pregnancy, without the need for tissue samples or reprogramming, would be advantageous. Amniotic fluid (AF) is a source of cells from multiple developing organs. Using single-cell analysis, we characterized the cellular identities present in human AF...
March 4, 2024: Nature Medicine