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https://www.readbyqxmd.com/read/28203018/-high-glucose-reduced-the-repair-function-of-kidney-stem-cells-conditional-medium-to-the-hypoxia-injured-renal-tubular-epithelium-cells
#1
G Yang, Q L Cheng, C L Li, Y L Jia, W Yue, X T Pei, Y Liu, J H Zhao, J DU, Q G Ao
OBJECTIVE: To evaluate the impacts of high glucose on the repair function of kidney stem cells (KSC) conditional medium to the hypoxia-injured renal tubular epithelium cells (RTEC). METHODS: KSC were isolated from the renal papilla in 4-week-Sprague-Dawley rats. The KSC were pretreated in media with high glucose (30 mmol/L) or with normal glucose (5.6 mmol/L), respectively. The supernatants of the pre-treated KSC were collected as the conditional media. The hypoxia/reoxygenation (H/R) model of rat RTEC was established using the NRK-52E cell line...
February 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28194187/effect-of-hypoxia-on-the-differentiation-and-the-self-renewal-of-metanephrogenic-mesenchymal-stem-cells
#2
Shaopeng Liu, Nana Song, Jianqiang He, Xiaofang Yu, Jia Guo, Xiaoyan Jiao, Xiaoqiang Ding, Jie Teng
Hypoxia is an important and influential factor in development. The embryonic kidney is exposed to a hypoxic environment throughout its development. The Wnt/β-catenin pathway plays vital roles in the differentiation and self-renewal of metanephrogenic mesenchymal stem cells (MMSCs) from which the kidney is derived. Thus, we hypothesized that hypoxia can regulate the differentiation and pluripotency of MMSCs through the Wnt/β-catenin pathway. To test this hypothesis, MMSCs from rats at embryonic day 18.5 were cultured in normoxic (21% O2) and hypoxic (1% O2) conditions...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28191781/direct-isolation-and-characterization-of-human-nephron-progenitors
#3
Stefano Da Sacco, Matthew E Thornton, Astgik Petrosyan, Maria Lavarreda-Pearce, Sargis Sedrakyan, Brendan H Grubbs, Roger E De Filippo, Laura Perin
Mature nephrons originate from a small population of uninduced nephrogenic progenitor cells (NPs) within the cap mesenchyme. These cells are characterized by the coexpression of SIX2 and CITED1. Many studies on mouse models as well as on human pluripotent stem cells have advanced our knowledge of NPs, but very little is known about this population in humans, since it is exhausted before birth and strategies for its direct isolation are still limited. Here we report an efficient protocol for direct isolation of human NPs without genetic manipulation or stepwise induction procedures...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28173878/the-effects-of-glomerular-and-tubular-renal-progenitors-and-derived-extracellular-vesicles-on-recovery-from-acute-kidney-injury
#4
Andrea Ranghino, Stefania Bruno, Benedetta Bussolati, Aldo Moggio, Veronica Dimuccio, Marta Tapparo, Luigi Biancone, Paolo Gontero, Bruno Frea, Giovanni Camussi
BACKGROUND: Mesenchymal stromal cells (MSCs) and renal stem/progenitors improve the recovery of acute kidney injury (AKI) mainly through the release of paracrine mediators including the extracellular vesicles (EVs). Several studies have reported the existence of a resident population of MSCs within the glomeruli (Gl-MSCs). However, their contribution towards kidney repair still remains to be elucidated. The aim of the present study was to evaluate whether Gl-MSCs and Gl-MSC-EVs promote the recovery of AKI induced by ischemia-reperfusion injury (IRI) in SCID mice...
February 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28164637/clinical-implications-of-plasma-n-acetyl-seryl-aspartyl-lysyl-proline-level-in-stable-kidney-transplant-recipients
#5
Yosuke Suzuki, Fumihiko Katagiri, Fuminori Sato, Kanako Fujioka, Yukie Sato, Takashi Fujioka, Yuhki Sato, Hiromitsu Mimata, Hiroki Itoh
BACKGROUND: N-acetyl-seryl-aspartyl-lysyl-proline (AcSDKP) is a natural inhibitor of pluripotent hematopoietic stem cell proliferation and is normally found in human plasma. Because AcSDKP is partially eliminated in urine, accumulation of AcSDKP due to chronic renal failure may cause anemia. However, the status of plasma AcSDKP level in stable kidney transplant recipients is unknown although some recipients develop anemia after kidney transplantation. In this study, we investigated the relationship between plasma AcSDKP-like immunoreactive substance (IS) level and clinical characteristics associated with renal anemia in stable kidney transplant recipients...
July 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28135776/long-term-renal-outcome-after-allogeneic-haemopoietic-stem-cell-transplant-a-comprehensive-analysis-of-risk-factors-in-an-asian-patient-population
#6
Wei Zhou, Rehena Sultana, Colin Diong, Yeow-Tee Goh, Sathish Gopalakrishnan, Aloysius Ho, William Hwang, Liang-Piu Koh, Mickey Koh, Yvonne Loh, Patrick Tan, Yeh-Ching Linn
Allogeneic haemopoietic stem cell transplantation (allo-HSCT) poses a significant challenge to renal function due to multiple drug- and complication-related renal toxicity. In this single center series of 216 adult Asian patients with a long and complete follow up, 41 developed chronic kidney disease (CKD) giving a cumulative incidence of 19.0% at 25 years (median follow up duration 7.84 years, range 2.0 - 27.7 years), but only 2 of the 41 patients reaching stage 4 CKD and another 2 requiring dialysis. In contrast, acute kidney injury occurred in most patients, where glomerular filtration rate (GFR) suffered a mean fall of 50ml/min/1...
January 30, 2017: Clinical Transplantation
https://www.readbyqxmd.com/read/28129785/human-umbilical-cord-derived-mesenchymal-stromal-cells-protect-against-premature-renal-senescence-resulting-from-oxidative-stress-in-rats-with-acute-kidney-injury
#7
Camila Eleuterio Rodrigues, José Manuel Condor Capcha, Ana Carolina de Bragança, Talita Rojas Sanches, Priscila Queiroz Gouveia, Patrícia Aparecida Ferreira de Oliveira, Denise Maria Avancini Costa Malheiros, Rildo Aparecido Volpini, Mirela Aparecida Rodrigues Santinho, Bárbara Amélia Aparecida Santana, Rodrigo do Tocantins Calado, Irene de Lourdes Noronha, Lúcia Andrade
BACKGROUND: Mesenchymal stromal cells (MSCs) represent an option for the treatment of acute kidney injury (AKI). It is known that young stem cells are better than are aged stem cells at reducing the incidence of the senescent phenotype in the kidneys. The objective of this study was to determine whether AKI leads to premature, stress-induced senescence, as well as whether human umbilical cord-derived MSCs (huMSCs) can prevent ischaemia/reperfusion injury (IRI)-induced renal senescence in rats...
January 28, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28117405/biological-membrane-packed-mesenchymal-stem-cells-treat-acute-kidney-disease-by-ameliorating-mitochondrial-related-apoptosis
#8
Xiaodong Geng, Quan Hong, Weiwei Wang, Wei Zheng, Ou Li, Guangyan Cai, Xiangmei Chen, Di Wu
The mortality of rhabdomyolysis-induced AKI remains high because no effective therapy exists. We investigated a new therapeutic method using MSCs. The aim of this study was to investigate the therapeutic potential and anti-apoptotic mechanisms of action of MSCs in the treatment of AKI induced by glycerol in vivo and in vitro. We used Duragen as a biological membrane to pack MSCs on the glycerol-injured renal tissue in vivo. The anti-apoptotic mechanism was investigated. In vitro, HK-2 cells were incubated with ferrous myoglobin and MSCs-conditioned medium, followed by cell proliferation and apoptosis assays...
January 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28116543/icariin-combined-with-human-umbilical-cord-mesenchymal-stem-cells-significantly-improve-the-impaired-kidney-function-in-chronic-renal-failure
#9
Wen Li, Li Wang, Xiaoqian Chu, Huantian Cui, Yuhong Bian
At present, the main therapy for chronic renal failure (CRF) is dialysis and renal transplantation, but neither obtains satisfactory results. Human umbilical cord mesenchymal stem cells (huMSCs) are isolated from the fetal umbilical cord which has a high self-renewal and multi-directional differentiation potential. Icariin (ICA), a kidney-tonifying Chinese Medicine can enhance the multipotency of huMSCs. Therefore, this work seeks to employ the use of ICA-treated huMSCs for the treatment of chronic renal failure...
January 23, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28105130/biological-characterization-of-sheep-kidney-derived-mesenchymal-stem-cells
#10
Meng Ji, Chunyu Bai, Lu Li, Ya'Nan Fan, Caiyun Ma, Xiangchen Li, Weijun Guan
The aim of the present study was to isolate, culture and characterize sheep metanephric mesenchymal stem cells (MMSCs). The MMSCs were isolated from the kidney tissue of six-week-old sheep fetus. This study investigated whether primary MMSCs could be grown for 26 passages and expressed Oct-4, which is involved in the self-renewal of undifferentiated pluripotent stem cells. The MMSCs also expressed the renal lineage marker gene PAX2, and mesenchymal cell marker genes CD44, FN1 and VIM. Expression of these genes was detected using immunofluorescence and reverse transcription-polymerase chain reaction assays...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28092863/adipose-derived-stem-cells-ameliorate-renal-interstitial-fibrosis-through-inhibition-of-emt-and-inflammatory-response-via-tgf-%C3%AE-1-signaling-pathway
#11
Yan Song, Changliang Peng, Shasha Lv, Jing Cheng, Shanshan Liu, Qing Wen, Guangju Guan, Gang Liu
Adipose-derived stem cells (ADSCs) have been successfully used to treat acute kidney injury or acute renal failure. However, the effect of ADSCs on treating renal interstitial fibrosis remains unknown. Here, we assessed the therapeutic efficacy of ADSCs on renal interstitial fibrosis induced by unilateral ureter obstruction (UUO) and explored the potential mechanisms. After 7days of UUO, rats were injected with ADSCs (5×10(6)) or vehicle via tail vein. We found that ADSCs administration significantly ameliorated renal interstitial fibrosis, the occurrence of epithelial-mesenchymal transition (EMT) and inflammatory response...
January 13, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28092350/long-term-renal-survival-in-patients-undergoing-t-cell-depleted-versus-conventional-hematopoietic-stem-cell-transplants
#12
I G Glezerman, S Devlin, M Maloy, M Bui, E A Jaimes, S A Giralt, A A Jakubowski
Calcineurin inhibitor (CNI)-sparing T-cell depleted (TCD) hematopoietic stem cell transplants (HSCTs) are presumed to be less nephrotoxic than conventional HSCTs. We evaluated incidence and risk factors for kidney failure and chronic kidney disease (CKD) in 231 TCD and 212 conventional HSCT recipients. Kidney failure required a median glomerular filtration rate (GFR) <60 ml/min/1.73 m(2) for ⩾100 days anytime after 180-days post-HSCT. Two-year cumulative incidence (CI) of kidney failure was 42% in the conventional versus 31% in the TCD group (P=0...
January 16, 2017: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/28089754/stem-cell-therapy-an-emerging-modality-in-glomerular-diseases
#13
REVIEW
Umang G Thakkar, Aruna V Vanikar, Hargovind L Trivedi
The kidney has been considered a highly terminally differentiated organ with low proliferative potential and thus unlikely to undergo regeneration. Glomerular disease progresses to end-stage renal disease (ESRD), which requires dialysis or renal transplantation for better quality of life for patients with ESRD. Because of the shortage of implantable kidneys and complications such as immune rejection, septicemia and toxicity of immunosuppression, kidney transplantation remains a challenge. Therapeutic options available for glomerular disease include symptomatic treatment and strategies to delay progression...
January 12, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28070858/exosome-and-microvesicle-enriched-fractions-isolated-from-mesenchymal-stem-cells-by-gradient-separation-showed-different-molecular-signatures-and-functions-on-renal-tubular-epithelial-cells
#14
Federica Collino, Margherita Pomatto, Stefania Bruno, Rafael Soares Lindoso, Marta Tapparo, Wen Sicheng, Peter Quesenberry, Giovanni Camussi
Several studies have suggested that extracellular vesicles (EVs) released from mesenchymal stem cells (MSCs) may mediate MSC paracrine action on kidney regeneration. This activity has been, at least in part, ascribed to the transfer of proteins/transcription factors and different RNA species. Information on the RNA/protein content of different MSC EV subpopulations and the correlation with their biological activity is currently incomplete. The aim of this study was to evaluate the molecular composition and the functional properties on renal target cells of MSC EV sub-populations separated by gradient floatation...
January 9, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28070037/-bm-mscs-from-wuzhishan-mini-pigs-delay-the-progress-of-renal-fibrosis-induced-by-chronic-kidney-disease-through-%C3%A2-autocrine-hepatocyte-growth-factor-in-vitro
#15
Yang Xiang, Jiale Long, Jiansheng Xing, Yuanhui Gao, Qing Cheng, Yong Cai, Zhenxiang Liu, Shufang Zhang, Lie Chen, Chao Yang, Zhiming Bai
To isolate bone marrow mesenchymal stem cells (BM-MSCs) and establish the model of chronic kidney disease (CKD) of Wuzhishan (WZS) mini-pig, and to study the repairment effect of BM-MSCs on CKD-induced renal fibrosis in vitro.
 Methods: Density gradient method was used to isolate and culture BM-MSCs. The cells were verified by morphology, phenotype, differentiation and so on. The left partial ureteral obstruction (LPUUO) was used to establish the CKD model, which was evaluated by B-ultrasound, single-photon emission computed tomography (SPECT), HE and Masson staining...
December 28, 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28056882/functional-significance-of-cd105-positive-cells-in-papillary-renal-cell-carcinoma
#16
Damian Matak, Klaudia K Brodaczewska, Cezary Szczylik, Irena Koch, Adam Myszczyszyn, Monika Lipiec, Slawomir Lewicki, Lukasz Szymanski, Robert Zdanowski, Anna M Czarnecka
BACKGROUND: CD105 was postulated as a renal cell carcinoma (RCC) stem cell marker, and CD133 as a putative RCC progenitor. Hypoxia, a natural microenvironment that prevails in tumors, was also incorporated into the study, especially in terms of the promotion of hypothetical stem-like cell properties. METHODS: Within this study, we verify the existence of CD105+ and CD133+ populations in selected papillary subtype RCC (pRCC) cell lines. Both populations were analyzed for correlation with stem-like cell properties, such as stemness gene expression, and sphere and colony formation...
January 5, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28017905/stem-cell-derived-kidney-cells-and-organoids-recent-breakthroughs-and-emerging-applications
#17
REVIEW
Jacqueline Kai Chin Chuah, Daniele Zink
The global rise in the numbers of kidney patients and the shortage in transplantable organs have led to an increasing interest in kidney-specific regenerative therapies, renal disease modelling and bioartificial kidneys. Sources for large quantities of high-quality renal cells and tissues would be required, also for applications in in vitro platforms for compound safety and efficacy screening. Stem cell-based approaches for the generation of renal-like cells and tissues would be most attractive, but such methods were not available until recently...
December 23, 2016: Biotechnology Advances
https://www.readbyqxmd.com/read/27990015/the-renal-fanconi-syndrome-in-cystinosis-pathogenic-insights-and-therapeutic-perspectives
#18
REVIEW
Stephanie Cherqui, Pierre J Courtoy
Cystinosis is an autosomal recessive metabolic disease that belongs to the family of lysosomal storage disorders. It is caused by a defect in the lysosomal cystine transporter, cystinosin, which results in an accumulation of cystine in all organs. Despite the ubiquitous expression of cystinosin, a renal Fanconi syndrome is often the first manifestation of cystinosis, usually presenting within the first year of life and characterized by the early and severe dysfunction of proximal tubule cells, highlighting the unique vulnerability of this cell type...
February 2017: Nature Reviews. Nephrology
https://www.readbyqxmd.com/read/27987038/efficient-genome-editing-of-differentiated-renal-epithelial-cells
#19
Alexis Hofherr, Tilman Busch, Nora Huber, Andreas Nold, Albert Bohn, Amandine Viau, Frank Bienaimé, E Wolfgang Kuehn, Sebastian J Arnold, Michael Köttgen
Recent advances in genome editing technologies have enabled the rapid and precise manipulation of genomes, including the targeted introduction, alteration, and removal of genomic sequences. However, respective methods have been described mainly in non-differentiated or haploid cell types. Genome editing of well-differentiated renal epithelial cells has been hampered by a range of technological issues, including optimal design, efficient expression of multiple genome editing constructs, attainable mutation rates, and best screening strategies...
February 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/27982115/generation-of-kidney-tubular-organoids-from-human-pluripotent-stem-cells
#20
Shintaro Yamaguchi, Ryuji Morizane, Koichiro Homma, Toshiaki Monkawa, Sayuri Suzuki, Shizuka Fujii, Muneaki Koda, Ken Hiratsuka, Maho Yamashita, Tadashi Yoshida, Shu Wakino, Koichi Hayashi, Junichi Sasaki, Shingo Hori, Hiroshi Itoh
Recent advances in stem cell research have resulted in methods to generate kidney organoids from human pluripotent stem cells (hPSCs), which contain cells of multiple lineages including nephron epithelial cells. Methods to purify specific types of cells from differentiated hPSCs, however, have not been established well. For bioengineering, cell transplantation, and disease modeling, it would be useful to establish those methods to obtain pure populations of specific types of kidney cells. Here, we report a simple two-step differentiation protocol to generate kidney tubular organoids from hPSCs with direct purification of KSP (kidney specific protein)-positive cells using anti-KSP antibody...
December 16, 2016: Scientific Reports
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