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Xiaoyan Liu, Xiaoxin Hu, Xinying Zhang, Xueping Chen, Jing Chen, Xiaoyu Yuan
A complex mix of organic pollutants and heavy metal made the remediation of contaminated wetlands more difficult. Few research focus on the remediation for pyrene enhanced by chemical reagents and pyrene degrading bacteria in the nickel co-contaminated soil. In this paper, the effect of chemical reagents (nitrilotriacetic acid and alkyl polyglucoside) and Bacillus subtilis on pyrene dissipation in phytoremediation of nickel co-contaminated soil by Scirpus triqueter was investigated. Similar seedlings of Scirpus triqueter were moved to uncontaminated soil and pyrene-nickel co-contaminated soil...
February 14, 2018: Ecotoxicology and Environmental Safety
Diego A Díaz-Dinamarca, José I Jerias, Daniel A Soto, Jorge A Soto, Natalia V Díaz, Yessica Y Leyton, Rodrigo A Villegas, Alexis M Kalergis, Abel E Vásquez
Group B Streptococcus (GBS) is the leading cause of neonatal meningitis and a common pathogen in livestock and aquaculture industries around the world. Conjugate polysaccharide and protein-based vaccines are under development. The surface immunogenic protein (SIP) is a conserved protein in all GBS serotypes and has been shown to be a good target for vaccine development. The expression of recombinant proteins in Escherichia coli cells has been shown to be useful in the development of vaccines, and the protein purification is a factor affecting their immunogenicity...
February 13, 2018: Molecular Biotechnology
Vishakha A Pawar, Anil S Prajapati, Rekha C Akhani, Darshan H Patel, R B Subramanian
A thermostable keratinase designated as KBALT was purified from Bacillus altitudinis RBDV1 from a poultry farm in Gujarat, India. The molecular weight of the native KBALT (nKBALT) purified using ammonium sulfate and ion exchange and gel permeation chromatography with a 40% yield and 80-fold purification was estimated to be ~ 43 kDa. The gene for KBALT was successfully cloned, sequenced and expressed in Escherichia coli. Recombinant KBALT (rKBALT) when purified using a single step Ni-NTA His affinity chromatography achieved a yield of 38...
February 2018: 3 Biotech
Yeong-Jun Park, Byung Kwon Jung, Sung-Jun Hong, Gun-Seok Park, Jerald Conrad Ibal, HuyQuang Pham, Jae-Ho Shin
The enzyme xylose isomerase (EC, XI) is responsible for theconversion of an aldose to ketose, especially xylose to xylulose. Owing to the ability of XI to isomerize glucose to fructose, this enzyme is used in the food industry to prepare high-fructose corn syrup. Therefore, we studiedthe characteristics of XI from Anoxybacillus kamchatkensis G10, a thermophilic bacterium. First, a gene coding for XI (xylA) was insertedinto the pET-21a (+) expression vector and the construct was transformed into an Escherichia coli competent cell BL21 (DE3)...
February 13, 2018: Journal of Microbiology and Biotechnology
Zuochen Yu, Benwei Zhu, Wenxia Wang, Haidong Tan, Heng Yin
A new oligoalginate lyase encoding gene, designed oal17A, was cloned from marine bacterium Vibrio sp. W13, and then expressed in Escherichia coli. The recombinant Oal17A was purified by NTA-Ni resin with maximal activity at 30 °C and pH 7.0. Oal17A exhibited broad substrate specificity, and preferred to degrade alginate than polyM or polyG into monosaccharide acid. The specific activity of Oal17A toward alginate, polyM and polyG was 21.14 U/mg, 12.31 U/mg and 7.43 U/mg, respectively. With features of high-level expression and broad substrate specificity, Oal17A would be a potential tool for alginate monomer production process of alginate utilizing for biofuels and bioethanol production...
February 8, 2018: International Journal of Biological Macromolecules
Basilius Thalmann, Urs von Gunten, Ralf Kaegi
Ozonation can be applied to mitigate the discharge of organic micropollutants from municipal wastewater treatment plants (WWTPs) to the aquatic environment. The toxicity of metals also present in WWTP effluents strongly depends on their speciation. Therefore, knowledge on the change of the metal speciation during ozonation of a WWTP effluent is essential to assess possible negative impacts. The kinetics and the stoichiometries of the reactions of ozone with three metal sulfides (ZnS, CuS and CdS) and metal-ethylenediaminetetraacetate (EDTA)/nitriloriacetic acid (NTA) complexes of Cu(II), Cd(II), Ni(II), Zn(II), Mg(II) and Pb(II) were investigated...
February 6, 2018: Water Research
Zhaohui Meng, Song Wang, Lijuan Wang, Hongjiang Hou
N-Doped carbon coated Li2ZnTi3O8 (LZTO@C-N) anodes have been successfully synthesized via a simple NTA-assisted solid-state method using NTA as C and N sources, as well as a chelating agent. It is shown that the N element can be doped into carbon. The N-doped carbon greatly benefits the electrochemical performance of LZTO@C-N. The initial capacities of 276.5, 271.3 and 231.9 mA h g-1 are delivered at 2, 3 and 5 A g-1, respectively, for LZTO@C-N-2 with a carbon content of 4.89%. 186.0, 177.3 and 159.7 mA h g-1 are still maintained at the 100th cycle, respectively...
February 7, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Monika Golda-Cepa, Paulina Chytrosz, Aleksandra Chorylek, Andrzej Kotarba
A facile one-step sonochemical method was employed for the first time for gentamicin nanoparticles (GNPs) fabrication and embedding into the surface of parylene C implant coating. The developed system was thoroughly characterized in terms of particle size (NTA, STEM/EDX), surface dispersion (IR-image) and drug release kinetics (UV-Vis). It was revealed that the optimization of the applied ultrasound conditions resulted in the formation of GNPs with an average size in the narrow range of 30-70 nm and their docking into the parylene C nanopores, while the molecular structure of the antibiotic was preserved as confirmed by the FTIR spectra...
January 30, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
Yu Larpin, Frank Oechslin, Philippe Moreillon, Grégory Resch, José Manuel Entenza, Stefano Mancini
The recent rise of multidrug-resistant Gram-negative bacteria represents a serious threat to public health and makes the search for novel effective alternatives to antibiotics a compelling need. Bacteriophage (Phage) lysins are enzymes that hydrolyze the cell wall of bacteria and represent a promising alternative to tackle this ever-increasing problem. Despite their use is believed to be restricted to Gram-positive bacteria, recent findings have shown that they can also be used against Gram-negative bacteria...
2018: PloS One
N D Vlieland, M R Nejadnik, H Gardarsdottir, S Romeijn, A S Sediq, M L Bouvy, A C G Egberts, B J F van den Bemt, W Jiskoot
PURPOSE: To measure aggregate and particle formation in tumor necrosis factor-alpha (TNF-α) inhibitors etanercept, adalimumab and certolizumab pegol product samples after exposure to freezing temperature conditions similar to storage conditions previously observed in patients' homes. METHODS: TNF-α inhibitors in their original primary and secondary packaging were exposed to 32 freeze-thaw cycles (-10°C for 120min/5°C for 60 min) or continuous low storage temperature (-20°C for 96 h) before thawing at 2-8°C...
February 5, 2018: Pharmaceutical Research
Zahra Sadat Hosseini, Jafar Amani, Fahimeh Baghbani Arani, Shahram Nazarian, Mohammad Javad Motamedi, Fatemeh Shafighian
Purpose: Escherichia coli O157:H7 is one of the most important pathogens which create hemorrhagic colitis and hemolytic uremic syndrome in human. It is one of the most prevalent causes of diarrhea leading to death of many people every year. The first diagnosed gene in the locus of enterocyte effacement pathogenicity island is eae gene. The product of this gene is a binding protein called intimin belonging to the group of external membrane proteins regarded as a good stimulants of the immune system...
January 2018: Clinical and Experimental Vaccine Research
Zhuyu Sun, Chaojie Zhang, Lu Xing, Qi Zhou, Wenbo Dong, Michael R Hoffmann
Perfluorooctane sulfonate (PFOS) is a toxic, bioaccumulative and highly persistent anthropogenic chemical. Hydrated electrons (eaq-) are potent nucleophiles that can effectively decompose PFOS. In previous studies, eaq- are mainly produced by photoionization of aqueous anions or aromatic compounds. In this study, we proposed a new photolytic strategy to generate eaq- and in turn decompose PFOS, which utilizes nitrilotriacetic acid (NTA) as a photosensitizer to induce water photodissociation and photoionization, and subsequently as a scavenger of hydroxyl radical (∙OH) to minimize the geminate recombination between ∙OH and eaq-...
February 3, 2018: Environmental Science & Technology
Akilandeswari Gopalan, Raghu Bhagavat, Nagasuma Chandra, Savithri Handanahal Subbarao, Alamelu Raja, Ramalingam Bethunaickan
Mycobacterium tuberculosis, the causative agent of tuberculosis disease, is one among the deadliest pathogens in the world. Due to long treatment regimen, HIV co-infection, persistence of bacilli in latent form and development of XDR and TDR strains of Mtb, tuberculosis has posed serious concerns for managing the disease, and calls for discovery of new drugs and drug targets. Using a computational pipeline involving analysis of the structural models of the Mtb proteome and an analysis of the ATPome, followed by a series of filters to identify druggable proteins, solubility and length of the protein, several candidate proteins were shortlisted...
February 12, 2018: Biochemical and Biophysical Research Communications
Luiz H Marques, Antonio C Santos, Boris A Castro, Nicholas P Storer, Jonathan M Babcock, Miles D Lepping, Verissimo Sa, Valéria F Moscardini, Dwain M Rule, Odair A Fernandes
Field-scale studies that examine the potential for adverse effects of Bt crop technology on non-target arthropods may supplement data from laboratory studies to support an environmental risk assessment. A three year field study was conducted in Brazil to evaluate potential for adverse effects of cultivating soybean event DAS-81419-2 that produces the Cry1Ac and Cry1F proteins. To do so, we examined the diversity and abundance of non-target arthropods (NTAs) in Bt soybean in comparison with its non-Bt near isoline, with and without conventional insecticide applications, in three Brazilian soybean producing regions...
2018: PloS One
Shibao He, Yaru Yin, Xi Zheng, Dongdong Guo, Jia Xin, Yong Zhu
The head of the silkworm is a nerve center and a sense organ, contains antennaes and sensory hair, feels the outside signal, and responds to the external signal delivered to the brain. Juvenile hormone is mainly synthesized and secreted by corpora allata, and it needs to be played with the aid of the hormone binding protein, because the juvenile hormone binding protein is the carrier of juvenile hormone transport and plays a functional in vivo, they have an extremely important function in insects. The objective of this study is to screened and identify a novel BmTOL proteins that it has a conserved structure of the juvenile hormone binding protein family by SilkDB and NCBI database...
January 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Pirudee Tuntrakool, Suttipun Keawsompong
Endo-1,4-β-mannanase is an enzyme that can catalyze the random hydrolysis of β-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans and offers many applications in different biotechnology industries. Purification and kinetic properties of the endo-1,4-β-mannanase from recombinant Escherichia coli strain KMAN-3 were examined. Recombinant β-mannanase (KMAN-3) was purified 50.5 fold using Ni-NTA Agarose resin and specific activity of 11900 U/mg protein was obtained. Purified KMAN-3 showed a single band on SDS-PAGE with a molecular weight of 43 kDa...
January 26, 2018: Protein Expression and Purification
H Y Huang, L Xiao, L L Bi, Y Gao, X R Kong, R Du, B Y Li, Y Han, W H Zhang, B Y Shi
Objective: Virus infection is a common complication of transplantation.With the research and application of exosome is becoming more popular, this study focused on whether the virus particles and nucleic acids exist in the exosomes extracted from the plasma of recipients with virus infection after renal transplantation. Methods: A total of 10 independent transplantation recipients at Institute of Organ Transplantation, 309th Hospital of Chinese People's Liberation Army from January 2015 to July 2017 were studied in this study...
January 16, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Neda Soleimani, Baharak Farhangi, Masoumeh Tavakoli Yaraki
Breast cancer imposes a considerable amount of cancer-related mortality and morbidity among women worldwide. Many efforts are in progress to reduce the disease burden and amongst the bacterial-based products received considerable attention as potential anti-cancer drugs. In the present study, the effect of recombinant pro-inflammatory outer membrane protein (HopH) of Helicobacter pylori on the angiogenic factor and tumor development in metastatic breast cancer model was evaluated. The HopH gene was cloned into Pet28a vector, induced by IPTG and expressed and purified by Ni-NTA affinity chromatography...
December 2017: Acta Medica Iranica
D Wasserberg, J Cabanas-Danés, V Subramaniam, J Huskens, P Jonkheijm
We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.
January 26, 2018: Chemical Communications: Chem Comm
Swathine Chandrashekaran, Srija Karthikeyan, Arvind Balakrishnan, Sreepriya Nair, Satheesh Kumar Kumaran Madathiparambil, Jane Jose Vattathara, Krishnakumar N Menon
Quinine dihydropteridinereductase (QDPR) is involved in the synthesis of tetradihydrobiopteridine (BH4) that serve as cofactor for many aromatic hydroxylases including induced nitric oxide synthase (NOS) leading to NO production. Increased activity of QDPR has been associated with decrease levels of TGF-β, a cytokine that regulates the immune response and that elevated levels of NO has been associated with neurodegenerative diseases. Thus, expression of QDPR in astrocytes is essential to study the pathological changes observed in many neurodegenerative disorders...
January 17, 2018: International Journal of Biological Macromolecules
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