keyword
MENU ▼
Read by QxMD icon Read
search

NTA

keyword
https://www.readbyqxmd.com/read/27928487/immunoreactivity-evaluation-of-a-new-recombinant-chimeric-protein-against-brucella-in-the-murine-model
#1
Abbas Abdollahi, Shahla Mansouri, Jafar Amani, Mahdi Fasihi-Ramandi, Mohammad Moradi
BACKGROUND AND OBJECTIVES: Brucellosis is an important health problem in developing countries and no vaccine is available for the prevention of infection in humans. Because of clinically infectious diseases and their economic consequences in human and animals, designing a proper vaccine against Brucella is desirable. In this study, we evaluated the immune responses induced by a designed recombinant chimera protein in murine model. MATERIALS AND METHODS: Three immunodominant antigens of Brucella have been characterized as potential immunogenic and protective antigens including: trigger factor (TF), Omp31 and Bp26 were fused together by EAAAK linkers to produce a chimera (structure were designed in silico), which was synthesized, cloned, and expressed in E...
June 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/27920885/cloning-and-expression-of-soluble-recombinant-hiv-1-crf35-protease-hp-thioredoxin-fusion-protein
#2
Asaad Azarnezhad, Zohreh Sharifi, Rahmatollah Seyedabadi, Arshad Hosseini, Behrooz Johari, Mahsa Sobhani Fard
BACKGROUND: As a drug target and an antigenic agent, HIV-1 protease (HIV-1 PR) is at the center of attention for designing anti-AIDS inhibitors and diagnostic tests. In previous studies, the production of the recombinant protease has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of protease in E. coli and investigation of its immunoreactivity. METHODS: Protease coding region was isolated from the serum of an infected individual, amplified by RT-PCR and cloned into PTZ57R using TA-cloning...
October 2016: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/27917276/preparation-and-characterization-of-a-novel-nanobody-against-t-cell-immunoglobulin-and-mucin-3-tim-3
#3
Vida Homayouni, Mazdak Ganjalikhani-Hakemi, Abbas Rezaei, Hossein Khanahmad, Mahdi Behdani, Fatemeh Kazemi Lomedasht
OBJECTIVES: As T-cell immunoglobulin and mucin domain 3 (TIM-3) is an immune regulatory molecule; its blocking or stimulating could alter the pattern of immune response towards a desired condition. Based on the unique features of nanobodies, we aimed to construct an anti-TIM-3 nanobody as an appropriate tool for manipulating immune responses for future therapeutic purposes. MATERIALS AND METHODS: We immunized a camel with TIM-3 antigen and then, synthesized a VHH phage: mid library from its B cell's transcriptome using nested PCR...
November 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27915002/label-free-histamine-detection-with-nanofluidic-diodes-through-metal-ion-displacement-mechanism
#4
Mubarak Ali, Patricio Ramirez, Ivana Duznovic, Saima Nasir, Salvador Mafe, Wolfgang Ensinger
We design and characterize a nanofluidic device for the label-free specific detection of histamine neurotransmitter based on a metal ion displacement mechanism. The sensor consists of an asymmetric polymer nanopore fabricated via ion track-etching technique. The nanopore sensor surface having metal-nitrilotriacetic (NTA-Ni(2+)) chelates is obtained by covalent coupling of native carboxylic acid groups with Nα,Nα-bis(carboxymethyl)-l-lysine (BCML), followed by exposure to Ni(2+) ion solution. The BCML immobilization and subsequent Ni(2+) ion complexation with NTA moieties change the surface charge concentration, which has a significant impact on the current-voltage (I-V) curve after chemical modification of the nanopore...
November 28, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27890597/identification-eukaryotic-expression-and-structure-function-characterizations-of-%C3%AE-defensin-like-homologues-from-pelodiscus-sinensis
#5
Haining Yu, Xuelian Liu, Hui Wang, Lan Feng, Xue Qiao, Shasha Cai, Nannan Shi, Yipeng Wang
Defensins are a group of host defense peptides that play a central role in host innate immune responses. Here, 26 genes encoding β-defensin-like peptides have been identified for the first time in Pelodiscus sinensis using database mining approach. Phylogenetic study confirmed that β-defensins are fast evolving genes with high rates of sequence substitutions. The expression level of several selected genes in different tissues was examined by RT-PCR. Ps-BDs mainly adopt β-strands and/or α-helix conformations homology modeled by Rosetta program...
November 24, 2016: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/27881682/stabilization-of-luciferase-from-renilla-reniformis-using-random-mutations
#6
Megumi Shigehisa, Norie Amaba, Shigeki Arai, Chisato Higashi, Ryo Kawanabe, Ayano Matsunaga, Fina Amreta Laksmi, Masao Tokunaga, Matsujiro Ishibashi
We expressed luciferase (RLuc) from Renilla reniformis in Escherichia coli RLuc was purified using a Ni-NTA column and subsequently characterized. It was unstable in acidic solutions and at 30°C. To increase the stability of RLuc, the Rluc gene was randomly mutated using error-prone polymerase chain reaction. E. coli harboring the mutated gene was screened by detecting luminescence on a plate containing the substrate coelenterazine at 34°C. Three mutants, i.e. N264SS287P, N178D and F116LI137V, were obtained...
November 23, 2016: Protein Engineering, Design & Selection: PEDS
https://www.readbyqxmd.com/read/27881345/-extraction-and-identification-of-semen-derived-exosomes-using-peg6000
#7
Cheng Yang, Wen-Bin Guo, Wan-Song Zhang, Jun Bian, Jian-Kun Yang, Tao Qi, Chun-Yan Wang, Cun-Dong Liu
OBJECTIVE: To extract and identify semen-derived exosome using PEG6000. METHODS: Exosomes were extracted from semen specimens from 6 healthy volunteers with step-by-step centrifugations and ultracentrifugation prior to 8% PEG6000 enrichment. The extracted exosomes were characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and Western blotting. RESULTS: The pellets obtained were round or elliptic membrane vesicles 30 to 150 nm in diameter with intact double membranes and contained low electron density material...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27871884/structural-characterization-of-recombinant-human-fibroblast-growth-factor-receptor-2b-kinase-domain-upon-interaction-with-omega-fatty-acids
#8
Masoumeh Moghadasi, Dariush Ilghari, Majid Sirati-Sabet, Abbas Amini, Hamideh Asghari, Nematollah Gheibi
The mutated recombinant kinase domain of human fibroblast growth factor receptor 2b (hFGFR2b) is overexpressed and purified, and its structural changes upon the interaction with three unsaturated fatty acids (UFAs), oleic, linoleic and α-linolenic are studied. This interaction is investigated to find out about the folding and unfolding effect of unsaturated fatty acids on the kinase domain structure of hFGFR2b. Recombinant pLEICS-01 vectors, containing the mutated coding region of hFGFR2b, are expressed in the standard Escherichia coli BL21 (DE3) host cells and purified by Ni(2+)-NTA affinity chromatography...
November 18, 2016: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/27864159/high-level-expression-and-efficient-refolding-of-therapeutically-important-recombinant-human-interleukin-3-hil-3-in-e-%C3%A2-coli
#9
Vikas Kumar Dagar, Adivitiya, Yogender Pal Khasa
Human interleukin-3 (hIL-3) is a pleiotropic cytokine that stimulates the differentiation and proliferation of multipotent hematopoietic cells thus making it a therapeutically important molecule. In this study, its poor expression yield was improved by addressing various upstream bottlenecks in E. coli heterologous system. The codon-optimized hIL-3 gene was cloned under various signal sequences and solubility enhancer fusion tags for its hyper-expression under a strong T7 promoter. The optimization of shake flask expression studies resulted in a hIL-3 protein concentration of 225 mg/L in the form of inclusion bodies (IBs)...
November 15, 2016: Protein Expression and Purification
https://www.readbyqxmd.com/read/27863521/seroprevalence-of-bovine-theileriosis-in-northern-china
#10
Yaqiong Li, Zhijie Liu, Junlong Liu, Jifei Yang, Qian Li, Pengfei Guo, Guiquan Guan, Guangyuan Liu, Jianxun Luo, Hong Yin, Youquan Li
BACKGROUND: Bovine theileriosis is a common disease transmitted by ticks, and can cause loss of beef and dairy cattle worldwide. Here, an indirect enzyme-linked immunosorbent assay (iELISA) based on Theileria luwenshuni surface protein (TlSP) was developed and used to carry out a seroepidemiological survey of bovine theileriosis in northern China. METHODS: We used the BugBuster Ni-NTA His•Bind Purification Kit to purify recombinant TlSP (rTlSP), which was subsequently analyzed by Western Blotting to evaluate cross-reactivity with other pathogen-positive sera...
November 18, 2016: Parasites & Vectors
https://www.readbyqxmd.com/read/27854041/functional-analysis-of-plantaricin-e-and-its-mutant-by-heterologous-expression-in-escherichia-coli
#11
Fanqiang Meng, Xiaoyu Zhu, Fengxia Lu, Xiaomei Bie, Zhaoxin Lu
Plantaricins are a group of ribosomally synthesized antimicrobial peptides in Lactobacillus plantarum that exert antimicrobial activities against some foodborne pathogens. In this study, we observed that plantaricin E in L. plantarum 163 was missing 19 amino acids (plnE mutant amino acid sequence: FNRGGYNFGKSVRH, plnE amino acid sequence: FNRGGYNFGKSVRHVVDAIGSVAGIRGILKSIR). In order to study the effects of mutant plnE, plnE mutant genes with and without the signal peptide were cloned from the L. plantarum 163 genome, linked to the pET32a vector, and expressed via a fusion protein (thioredoxin) in Escherichia coli BL21 (DE3)...
November 16, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27829635/quantitative-determination-of-nta-and-other-chelating-agents-in-detergents-by-potentiometric-titration-with-copper-ion-selective-electrode
#12
Sana Ito, Masaki Morita
Quantitative analysis of nitrilotriacetate (NTA) in detergents by titration with Cu(2+) solution using a copper ion selective electrode was achieved. This method tolerates a wide range of pH and ingredients in detergents. In addition to NTA, other chelating agents, having relatively lower stability constants toward Cu(2+), were also qualified with sufficient accuracy by this analytical method for model detergent formulations. The titration process was automated by automatic titrating systems available commercially...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829207/ellagic-acid-inhibits-iron-mediated-free-radical-formation
#13
Luana T Dalvi, Daniel C Moreira, Roberto Andrade, Janini Ginani, Antonio Alonso, Marcelo Hermes-Lima
Polyphenols are reported to have some health benefits, which are link to their antioxidant properties. In the case of ellagic acid (EA), there is evidence that it has free radical scavenger properties and that it is able to form complexes with metal ions. However, information on a possible link between the formation of iron-EA complexes and their interference in Haber-Weiss/Fenton reactions was not yet determined. Thus, the present study investigated the in vitro antioxidant mechanism of EA in a system containing ascorbate, Fe(III) and different iron ligands (EDTA, citrate and NTA)...
October 21, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/27819794/memristive-behaviour-in-poly-acrylic-acid-coated-tio2-nanotube-arrays
#14
Daniele Conti, Andrea Lamberti, Samuele Porro, Paola Rivolo, Alessandro Chiolerio, Candido Fabrizio Pirri, Carlo Ricciardi
This work investigates titanium dioxide nanotube arrays (TiO2-NTA) grown by anodic oxidation as an active material for memristive applications. In particular, metal-insulator-metal structures made of vertically oriented amorphous TiO2-NTA grown on titanium foil were exploited in Ti/TiO2-NTA/Pt devices. The deposition of a polymeric thin film between NTA and top electrodes significantly improved the stability of the devices and increased by more than double the off/on resistance ratio. The resistive switching of TiO2-NTA samples crystallised by thermal annealing was also studied...
December 2, 2016: Nanotechnology
https://www.readbyqxmd.com/read/27798662/assessing-potential-impact-of-bt-eggplants-on-non-target-arthropods-in-the-philippines
#15
Mario V Navasero, Randolph N Candano, Desiree M Hautea, Randy A Hautea, Frank A Shotkoski, Anthony M Shelton
Studies on potential adverse effects of genetically engineered crops are part of an environmental risk assessment that is required prior to the commercial release of these crops. Of particular concern are non-target organisms (NTOs) that provide important ecosystem services. Here, we report on studies conducted in the Philippines over three cropping seasons with Bt eggplants expressing Cry1Ac for control of the eggplant fruit and shoot borer (EFSB), Leucinodes orbonalis, to examine potential effects on field abundance, community composition, structure and biodiversity of NTO's, particularly non-target arthropod (NTA) communities...
2016: PloS One
https://www.readbyqxmd.com/read/27792985/an-investigation-of-the-interactions-between-an-e-coli-bacterial-quorum-sensing-biosensor-and-chitosan-based-nanocapsules
#16
Xiaofei Qin, Christoph Engwer, Saaketh Desai, Celina Vila-Sanjurjo, Francisco M Goycoolea
We examined the interaction between chitosan-based nanocapsules (NC), with average hydrodynamic diameter ∼114-155nm, polydispersity ∼0.127, and ζ-potential ∼+50mV, and an E. coli bacterial quorum sensing reporter strain. Dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) allowed full characterization and assessment of the absolute concentration of NC per unit volume in suspension. By centrifugation, DLS, and NTA, we determined experimentally a "stoichiometric" ratio of ∼80 NC/bacterium...
October 17, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27777836/affinity-capturing-and-surface-enrichment-of-a-membrane-protein-embedded-in-a-continuous-supported-lipid-bilayer
#17
Anders Gunnarsson, Lisa Simonsson Nyström, Sabina Burazerovic, Jenny Gunnarsson, Arjan Snijder, Stefan Geschwindner, Fredrik Höök
Investigations of ligand-binding kinetics to membrane proteins are hampered by their poor stability and low expression levels, which often translates into sensitivity-related limitations impaired by low signal-to-noise ratios. Inspired by affinity capturing of water-soluble proteins, which utilizes water as the mobile phase, we demonstrate affinity capturing and local enrichment of membrane proteins by using a fluid lipid bilayer as the mobile phase. Specific membrane-protein capturing and enrichment in a microfluidic channel was accomplished by immobilizing a synthesized trivalent nitrilotriacetic acid (tris-NTA)-biotin conjugate...
October 2016: ChemistryOpen
https://www.readbyqxmd.com/read/27775592/prokaryotic-expression-purification-and-immunogenicity-in-rabbits-of-the-small-antigen-of-hepatitis-delta-virus
#18
Vera L Tunitskaya, Olesja V Eliseeva, Vladimir T Valuev-Elliston, Daria A Tyurina, Natalia F Zakirova, Olga A Khomich, Martins Kalis, Oleg E Latyshev, Elizaveta S Starodubova, Olga N Ivanova, Sergey N Kochetkov, Maria G Isaguliants, Alexander V Ivanov
Hepatitis delta virus (HDV) is a viroid-like blood-borne human pathogen that accompanies hepatitis B virus infection in 5% patients. HDV has been studied for four decades; however, the knowledge on its life-cycle and pathogenesis is still sparse. The studies are hampered by the absence of the commercially-available HDV-specific antibodies. Here, we describe a set of reproducible methods for the expression in E. coli of His-tagged small antigen of HDV (S-HDAg), its purification, and production of polyclonal anti-S-HDAg antibodies in rabbits...
October 20, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27773766/affinity-purification-of-bacterial-outer-membrane-vesicles-omvs-utilizing-a-his-tag-mutant
#19
Nathan J Alves, Kendrick B Turner, Kyle A DiVito, Michael A Daniele, Scott A Walper
To facilitate the rapid purification of bacterial outer membrane vesicles (OMVs), we developed two plasmid constructs that utilize a truncated, transmembrane protein to present an exterior histidine repeat sequence. We chose OmpA, a highly abundant porin protein, as the protein scaffold and utilized the lac promoter to allow for inducible control of the epitope-presenting construct. OMVs containing mutant OmpA-His6 were purified directly from Escherichia coli culture media on an immobilized metal affinity chromatography (IMAC) Ni-NTA resin...
October 20, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27771514/keepex-a-simple-dilution-protocol-for-improving-extracellular-vesicle-yields-from-urine
#20
M Puhka, M-E Nordberg, S Valkonen, A Rannikko, O Kallioniemi, P Siljander, T M Af Hällström
Urinary extracellular vesicles (EVs) are a promising source of biomarkers, which can be obtained in a non-invasive manner. However, the yield of EVs from urine samples may be insufficient for various analyses due to the entrapment of EVs by the Tamm-Horsfall protein (THP) meshwork. Here, we developed a simple dilution protocol to increase the urinary EV yield by disrupting the interaction between THP filaments and EVs with the help of alkaline pH and lowered ionic concentration. The integrity of the EVs and THP was assessed by electron microscopy...
October 19, 2016: European Journal of Pharmaceutical Sciences
keyword
keyword
46081
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"