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https://www.readbyqxmd.com/read/28338635/a-formaldehyde-sensor-based-on-molecularly-imprinted-polymer-on-a-tio%C3%A2-nanotube-array
#1
Xiaohui Tang, Jean-Pierre Raskin, Driss Lahem, Arnaud Krumpmann, André Decroly, Marc Debliquy
Today, significant attention has been brought to the development of sensitive, specific, cheap, and reliable sensors for real-time monitoring. Molecular imprinting technology is a versatile and promising technology for practical applications in many areas, particularly chemical sensors. Here, we present a chemical sensor for detecting formaldehyde, a toxic common indoor pollutant gas. Polypyrrole-based molecularly-imprinted polymer (PPy-based MIP) is employed as the sensing recognition layer and synthesized on a titanium dioxide nanotube array (TiO₂-NTA) for increasing its surface-to-volume ratio, thereby improving the sensor performance...
March 24, 2017: Sensors
https://www.readbyqxmd.com/read/28335675/development-and-application-of-an-indirect-enzyme-linked-immunosorbent-assay-using-recombinant-mag1-for-serodiagnosis-of-toxoplasma-gondii-in-dogs
#2
Xunhui Zhuo, Hongchao Sun, Zhi Zhang, Jiaqing Luo, Ying Shan, Aifang Du
Serologic tests are widely accepted and applied as means to detect anti Toxoplasma gondii immunoglobulin G antibodies(IgG). In this study, recombinant matrix antigen (MAG1) was induced by isopropyl-β-d-thiogalactoside(IPTG) and purified by nickel-nitrilotriacetic acid (Ni-NTA) purification system. We then developed and optimized an indirect enzyme-linked immunosorbent assay (ELISA) through checkerboard assays using serial dilutions of antigens and sera to assess the potential use of MAG1 in serologic detection of T...
March 23, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28330270/expression-and-purification-of-a-gene-encoding-a-9-7%C3%A2-kda-pe-protein-of-mycobacterium-avium-subsp-paratuberculosis
#3
S Chandra Sekar, P P Goswami, R Deb
Mycobacterium avium subsp. paratuberculosis (Map) contains PE family antigens which are Proline and glutamic acid rich and may play important role as T-cell antigens. In the present study, the Map 1507 ORF encoding 9.7 kDa PE protein was amplified by polymerase chain reaction and cloned into E. coli vector pQE30 UA. The recombinant plasmid designated as pQ PE was transformed into E. coli M15 cells and induced with IPTG revealed the high level expression of 11.9 kDa His-fusion protein as estimated by migration in 15 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330086/cloning-expression-purification-and-characterization-of-human-mitochondrial-carbonic-anhydrase-va
#4
Danish Idrees, Sudhir Kumar, Syed Abdul Arif Rehman, Samudrala Gourinath, Asimul Islam, Faizan Ahmad, Md Imtaiyaz Hassan
Carbonic anhydrase VA (CAVA) is a mitochondrial enzyme that catalyzes the reversible hydration of CO2 to produce HCO3(-) and proton. CAV is primarily involved in several biosynthetic processes such as ureagenesis, gluconeogenesis and lipogenesis by providing bicarbonate ion. Here, we report a new strategy for cloning, expression and purification for CAVA in the bacterial system followed by its biophysical characterization. The cDNA of CAVA, a 801 nucleotide long that encodes a 267-amino acid polypeptide of molecular mass of 30-kDa (excluding signal peptide), was sub-cloned in the expression vector pET21c and transformed into Escherichia coli strain BL21 (DE3) for expression...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28317857/enhanced-biennial-variability-in-the-pacific-due-to-atlantic-capacitor-effect
#5
Lei Wang, Jin-Yi Yu, Houk Paek
The El Niño-Southern Oscillation (ENSO) and the variability in the Pacific subtropical highs (PSHs) have major impacts on social and ecological systems. Here we present an Atlantic capacitor effect mechanism to suggest that the Atlantic is a key pacemaker of the biennial variability in the Pacific including that in ENSO and the PSHs during recent decades. The 'charging' (that is, ENSO imprinting the North Tropical Atlantic (NTA) sea surface temperature (SST) via an atmospheric bridge mechanism) and 'discharging' (that is, the NTA SST triggering the following ENSO via a subtropical teleconnection mechanism) processes alternate, generating the biennial rhythmic changes in the Pacific...
March 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28315745/production-of-low-expressing-recombinant-cationic-biopolymers-with-high-purity
#6
Xuguang Chen, Alireza Nomani, Niket Patel, Arash Hatefi
The growing complexity of recombinant biopolymers for delivery of bioactive agents requires the ability to control the biomaterial structure with high degree of precision. Genetic engineering techniques have provided this opportunity to synthesize biomaterials in an organism such as E. coli with full control over their lengths and sequences. One class of such biopolymers is recombinant cationic biopolymers with applications in gene delivery, regenerative medicine and variety of other biomedical applications...
March 16, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28301074/high-throughput-inclusion-body-sizing-nano-particle-tracking-analysis
#7
Wieland N Reichelt, Andreas Kaineder, Markus Brillmann, Lukas Neutsch, Alexander Taschauer, Hans Lohninger, Christoph Herwig
The expression of pharmaceutical relevant proteins in E. coli frequently triggers inclusion body (IB) formation caused by protein aggregation. In literature, substantial effort has been invested into the quantification of IB size. But up to this date particle based methods to analyze physical IB properties of representative numbers of IBs lack sensitivity and/or lack orthogonal verification. Using high pressure freezing and automated freeze substitution for transmission electron microscopy (TEM) the cytosolic inclusion body structure was preserved within the cells...
March 16, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28296083/immobilization-of-pt-nanoparticles-via-rapid-and-reusable-electropolymerization-of-dopamine-on-tio2-nanotube-arrays-for-reversible-sers-substrates-and-nonenzymatic-glucose-sensors
#8
Jingsheng Cai, Jianying Huang, Mingzheng Ge, James Iocozzia, Zhiqun Lin, Ke-Qin Zhang, Yuekun Lai
Inspired by mussel-adhesion phenomena in nature, polydopamine (PDA) coatings are a promising route to multifunctional platforms for decorating various materials. The typical self-polymerization process of dopamine is time-consuming and the coatings of PDA are not reusable. Herein, a reusable and time-saving strategy for the electrochemical polymerization of dopamine (EPD) is reported. The PDA layer is deposited on vertically aligned TiO2 nanotube arrays (NTAs). Owing to the abundant catechol and amine groups in the PDA layer, uniform Pt nanoparticles (NPs) are deposited onto the TiO2 NTAs and can effectively prevent the recombination of electron-hole pairs generated from photo-electrocatalysis and transfer the captured electrons to participate in the photo-electrocatalytic reaction process...
March 10, 2017: Small
https://www.readbyqxmd.com/read/28294278/controlling-adsorption-and-passivation-properties-of-bovine-serum-albumin-on-silica-surfaces-by-ionic-strength-modulation-and-cross-linking
#9
Jae Hyeon Park, Tun Naw Sut, Joshua A Jackman, Abdul Rahim Ferhan, Bo Kyeong Yoon, Nam-Joon Cho
Understanding the physicochemical factors that influence protein adsorption onto solid supports holds wide relevance for fundamental insights into protein structure and function as well as for applications such as surface passivation. Ionic strength is a key parameter that influences protein adsorption, although how its modulation might be utilized to prepare well-coated protein adlayers remains to be explored. Herein, we investigated how ionic strength can be utilized to control the adsorption and passivation properties of bovine serum albumin (BSA) on silica surfaces...
March 15, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28286754/prokaryotic-expression-of-%C3%AE-13-giardin-gene-and-its-intracellular-localization-in-giardia-lamblia
#10
Xingang Yu, Auwalu Yusuf Abdullahi, Sheng Wu, Weida Pan, Xianli Shi, Wei Hu, Liping Tan, Kangxin Li, Zhen Wang, Guoqing Li
To study prokaryotic expression and subcellular localization of α-13 giardin in Giardia lamblia trophozoites, α-13 giardin gene was amplified and cloned into prokaryotic expression vector pET-28a(+). The positive recombinant plasmid was transformed into E. coli BL21(DE3) for expression by using IPTG and autoinduction expression system (ZYM-5052). The target protein was validated by SDS-PAGE and Western blotting and purified by Ni-NTA Resin. Rabbits were immunized with purified fusion proteins for preparation of polyclonal antibody; then the intracellular location of α-13 giardin was determined by fluorescence immunoassay...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28285404/exosomes-mediated-transfer-of-long-noncoding-rna-zfas1-promotes-gastric-cancer-progression
#11
Lei Pan, Wei Liang, Min Fu, Zhen-Hua Huang, Xia Li, Wen Zhang, Peng Zhang, Hui Qian, Peng-Cheng Jiang, Wen-Rong Xu, Xu Zhang
BACKGROUND: ZFAS1 is a newly identified long noncoding RNA (lncRNA) that promotes tumor growth and metastasis. Exosomes mediate cellular communications in cancer by transmitting active molecules. The presence of ZFAS1 in the circulating exosomes and the roles of exosomal ZFAS1 in gastric cancer (GC) remains unknown. The aim of this study was to investigate the potential roles of exosomal ZFAS1 in GC. METHODS: The expression of ZFAS1 was examined in the tumor tissues, serum samples, serum exosomes of GC patients and cell lines using qRT-PCR...
March 11, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28274740/lipid-nanocapsules-maintain-full-integrity-after-crossing-a-human-intestinal-epithelium-model
#12
Emilie Roger, Jean-Christophe Gimel, Conor Bensley, Andrey S Klymchenko, Jean-Pierre Benoit
Lipid nanocapsules (LNCs) have demonstrated great potential for the oral delivery of drugs having very limited oral bioavailability (BCS class II, III and IV molecules). It has been shown previously that orally-administered LNCs can permeate through mucus, increase drug absorption by the epithelial tissue, and finally, increase drug bioavailability. However, even if transport mechanisms through mucus and the intestinal barrier have already been clarified, the preservation of particle integrity is still not known...
March 6, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28263879/ctenopharyngodon-idella-irf2-and-atf4-down-regulate-the-transcriptional-level-of-prkra
#13
Keyi Huang, Guoqin Qi, Zhicheng Sun, Xiancheng Liu, Xiaowen Xu, Haizhou Wang, Zhen Wu, Yiqi Wan, Chengyu Hu
PRKRA (interferon-inducible double-stranded RNA-dependent protein kinase activator A) is a protective protein which regulates the adaptation of cells to ER stress and virus-stimulated signaling pathways by activating PKR. In the present study, a grass carp (Ctenopharyngodon idella) PRKRA full-length cDNA (named CiPRKRA, KT891991) was cloned and identified. The full-length cDNA is comprised of a 5' UTR (36 bp), a 3' UTR (350 bp) and the longest ORF (882 bp) encoding a polypeptide of 293 amino acids. The deduced amino acid sequence of CiPRKRA contains three typical dsRNA binding motifs (dsRBM)...
March 2, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28260494/hemoglobin-and-myoglobin-as-reducing-agents-in-biological-systems-redox-reactions-of-globins-with-copper-and-iron-salts-and-complexes
#14
REVIEW
G B Postnikova, E A Shekhovtsova
In addition to reversible O2 binding, respiratory proteins of the globin family, hemoglobin (Hb) and myoglobin (Mb), participate in redox reactions with various metal complexes, including biologically significant ones, such as those of copper and iron. HbO2 and MbO2 are present in cells in large amounts and, as redox agents, can contribute to maintaining cell redox state and resisting oxidative stress. Divalent copper complexes with high redox potentials (E0, 200-600 mV) and high stability constants, such as [Cu(phen)2](2+), [Cu(dmphen)2](2+), and CuDTA oxidize ferrous heme proteins by the simple outer-sphere electron transfer mechanism through overlapping π-orbitals of the heme and the copper complex...
December 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28258552/isolation-and-functional-characterization-of-a-methyl-jasmonate-responsive-3-carene-synthase-from-lavandula-x-intermedia
#15
Ayelign M Adal, Lukman S Sarker, Ashley D Lemke, Soheil S Mahmoud
A methyl jasmonate responsive 3-carene synthase (Li3CARS) gene was isolated from Lavandula x intermedia and functionally characterized in vitro. Lavenders produce essential oils consisting mainly of monoterpenes, including the potent antimicrobial and insecticidal monoterpene 3-carene. In this study we isolated and functionally characterized a leaf-specific, methyl jasmonate (MeJA)-responsive monoterpene synthase (Li3CARS) from Lavandula x intermedia. The ORF excluding transit peptides encoded a 64.9 kDa protein that was expressed in E...
March 3, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28247879/rapid-and-specific-detection-of-cell-derived-microvesicles-using-a-magnetoresistive-biochip
#16
Solène Cherré, Elisabete Fernandes, José Germano, Tomás Dias, Susana Cardoso, Moisés S Piedade, Noemi Rozlosnik, Marta I Oliveira, Paulo P Freitas
Microvesicles (MVs) are a promising source of diagnostic biomarkers which have gained a wide interest in the biomedical and biosensing field. They can be interpreted as a "fingerprint" of various diseases. Nonetheless, MVs implementation into clinical settings has been hampered by the lack of technologies to accurately characterize, detect and quantify them. Here, we report the specific sensing and quantification of MVs from endothelial cells using a portable magnetoresistive (MR) biochip platform, in less than one hour and within physiologically relevant concentrations (1 × 10(8) MVs per ml)...
March 13, 2017: Analyst
https://www.readbyqxmd.com/read/28241021/extracellular-vesicles-mediate-signaling-between-the-aqueous-humor-producing-and-draining-cells-in-the-ocular-system
#17
Natalie Lerner, Sofia Avissar, Elie Beit-Yannai
PURPOSE: Canonical Wnt signaling is associated with glaucoma pathogenesis and intraocular pressure (IOP) regulation. Our goal was to gain insight into the influence of non-pigmented ciliary epithelium (NPCE)-derived exosomes on Wnt signaling by trabecular meshwork (TM) cells. The potential impact of exosomes on Wnt signaling in the ocular drainage system remains poorly understood. METHODS: Exosomes isolated from media collected from cultured NPCE cells by differential ultracentrifugation were characterized by dynamic light scattering (DLS), tunable resistive pulse sensing (TRPS), and nanoparticle tracking analysis (NTA), sucrose density gradient migration and transmission electron microscopy (TEM)...
2017: PloS One
https://www.readbyqxmd.com/read/28238785/zn-2-dependent-autocatalytic-activity-of-the-bordetella-pertussis-cyaa-hemolysin
#18
Veerada Raksanoh, Lalida Shank, Panchika Prangkio, Mattayaus Yentongchai, Somsri Sakdee, Chompounoot Imtong, Chanan Angsuthanasombat
Proteolytic degradation of the ∼100-kDa isolated RTX (Repeat-in-ToXin) subdomain (CyaA-RTX) of the Bordetella pertussis CyaA-hemolysin (CyaA-Hly) was evidently detected upon solely-prolonged incubation. Here, a truncated CyaA-Hly fragment (CyaA-HP/BI) containing hydrophobic and acylation regions connected with the first RTX block (BI1015-1088) was constructed as a putative precursor for investigating its potential autocatalysis. The 70-kDa His-tagged CyaA-HP/BI fragment which was over-expressed in Escherichia coli as insoluble aggregate was entirely solubilized with 4 M urea...
April 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28238128/nanoparticle-tracking-analysis-for-determination-of-hydrodynamic-diameter-concentration-and-zeta-potential-of-polyplex-nanoparticles
#19
David R Wilson, Jordan J Green
Nanoparticle tracking analysis (NTA) is a recently developed nanoparticle characterization technique that offers certain advantages over dynamic light scattering for characterizing polyplex nanoparticles in particular. Dynamic light scattering results in intensity-weighted average measurements of nanoparticle characteristics. In contrast, NTA directly tracks individual particles, enabling concentration measurements as well as the direct determination of number-weighted particle size and zeta-potential. A direct number-weighted assessment of nanoparticle characteristics is particularly useful for polydisperse samples of particles, including many varieties of gene delivery particles that can be prone to aggregation...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28235780/inhibitory-cortactin-nanobodies-delineate-the-role-of-nta-and-sh3-domain-specific-functions-during-invadopodium-formation-and-cancer-cell-invasion
#20
Laurence Bertier, Ciska Boucherie, Olivier Zwaenepoel, Berlinda Vanloo, Marleen Van Troys, Isabel Van Audenhove, Jan Gettemans
Cancer cells exploit different strategies to escape from the primary tumor, gain access to the circulation, disseminate throughout the body, and form metastases, the leading cause of death by cancer. Invadopodia, proteolytically active plasma membrane extensions, are essential in this escape mechanism. Cortactin is involved in every phase of invadopodia formation, and its overexpression is associated with increased invadopodia formation, extracellular matrix degradation, and cancer cell invasion. To analyze endogenous cortactin domain function in these processes, we characterized the effects of nanobodies that are specific for the N-terminal acidic domain of cortactin and expected to target small epitopes within this domain...
February 24, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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