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https://www.readbyqxmd.com/read/28428688/modulation-of-angiogenesis-proliferative-response-and-apoptosis-by-%C3%AE-sitosterol-in-rat-model-of-renal-carcinogenesis
#1
Ramalingam Sharmila, Ganapathy Sindhu
As expanded understanding of molecular tumor characteristics, which drive renal cancer growth and progression gives a promising future for renal carcinoma therapy. The objective of the present study was designed to examine the effect of β-sitosterol on a rat model of experimental renal carcinogenesis. Renal carcinogenesis was induced in rats treated with N-diethylnitrosamine (DEN; 200 mg/kg bw single i.p., injection) and ferric nitrilotriacetate (Fe-NTA; 9 mg Fe/kg bw i.p., twice a week for 16 weeks). β-sitosterol pretreatment (20 mg/kg bw in 0...
June 2017: Indian Journal of Clinical Biochemistry: IJCB
https://www.readbyqxmd.com/read/28427419/tnf-%C3%AE-promotes-extracellular-vesicle-release-in-mouse-astrocytes-through-glutaminase
#2
Kaizhe Wang, Ling Ye, Hongfang Lu, Huili Chen, Yanyan Zhang, Yunlong Huang, Jialin C Zheng
BACKGROUND: Extracellular vesicles (EVs) are membrane-contained vesicles shed from cells. EVs contain proteins, lipids, and nucleotides, all of which play important roles in intercellular communication. The release of EVs is known to increase during neuroinflammation. Glutaminase, a mitochondrial enzyme that converts glutamine to glutamate, has been implicated in the biogenesis of EVs. We have previously demonstrated that TNF-α promotes glutaminase expression in neurons. However, the expression and the functionality of glutaminase in astrocytes during neuroinflammation remain unknown...
April 20, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28417626/production-and-characterization-of-monoclonal-antibody-against-recombinant-virus-coat-protein-cp42
#3
Naeimeh Shibaei, Jafar Majidi, Khadijeh Razavi, Ali Asghar Karkhane, Nemat Sokhandan-Bashir, Leili Aghebati-Maleki
There are many studies related to the production of a ELISA kit for diagnosing virus infections. However, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. The purpose of this study was to check out if the anti-CP42 antibodies could be used as a diagnostic assay for detection of Grapevine fanleaf Virus (GFLV). In this study, recombinant GFLV coat protein gene related to selected antigenic determinants was inserted into pET-28a bacterial expression vector and the construct (pET-28a CP42) was cloned into E...
February 2017: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/28416498/deprescribing-anticholinergic-and-sedative-medicines-protocol-for-a-feasibility-trial-defeat-polypharmacy-in-residential-aged-care-facilities
#4
Nagham Ailabouni, Dee Mangin, Prasad S Nishtala
INTRODUCTION: Targeted deprescribing of anticholinergic and sedative medicines can lead to positive health outcomes in older people; as they have been associated with cognitive and physical functioning decline. This study will examine whether the proposed intervention is feasible at reducing the prescription of anticholinergic and sedative medicines in older people. METHODS AND ANALYSIS: The Standard Protocol Items: Recommendations for Interventional trials (SPIRIT checklist) was used to develop and report the protocol...
April 16, 2017: BMJ Open
https://www.readbyqxmd.com/read/28412830/expression-and-immunogenicity-of-vp40-protein-of-zebov
#5
Yinbiao Wang, Xiao Liu, Ling Tao, Pengwei Xu, Xia Gao, Huijun Li, Zhongzhi Yang, Weidong Wu
BACKGROUND: EBOV outbreaks continue to threaten the world due to the absence of effective vaccines and therapeutics. Easy-to-use and rapid diagnostic tests for EBOV are highly desired for prevention and control of the EVD epidemic. METHODS: Escherichia coli expression system was used to express VP40 protein of Zaire Ebola virus (ZEBOV) as water-soluble protein upon optimization of temperature, time, and IPTG concentration. VP40 protein was purified through Ni-NTA affinity chromatography and applied to immunize rabbits for immunogenicity analysis...
April 2017: Archives of Iranian Medicine
https://www.readbyqxmd.com/read/28410993/development-of-a-novel-recombinant-lhrh-fusion-protein-for-therapy-of-androgen-and-estrogen-dependent-cancers
#6
Jagdish C Gupta, Rohit S Hada, P Sahai, G P Talwar
LHRH based vaccines are promising candidates for therapy of androgen and estrogen dependent cancers. We report in this communication development of a novel recombinant protein vaccine candidate against LHRH. A synthetic gene was designed in which the codon sequence in the LHRH decapeptide was modified by substituting the codon for 6-glycine with that of l-leucine. Further the LHRH(6leu) gene was linked to heat-labile enterotoxin of E. coli (LTB) as carrier. This LHRH(6leu)-LTB gene was cloned into a prokaryotic expression vector under the control of inducible and strong bacteriophage T7 promoter to over-express LHRH(leu) fused to LTB as recombinant protein in E...
April 11, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28407595/proteo-lipobeads-to-encapsulate-cytochrome-c-oxidase-from-paracoccus-denitrificans
#7
Andreas F Geiss, Christina Bliem, Pinar Frank, Ciril Reiner-Rozman, Justin Kewney, Michael Boersch, Renate L C Naumann
Proteo-lipobeads (PLBs) are investigated as cell-free model systems to encapsulate membrane proteins such as ion channels and transporters. PLBs are based on nickel nitrile tri-acetic acid (Ni-NTA)-functionalized agarose beads, onto which membrane proteins (MP) are bound via histidine(his)-tag. Composite beads thus obtained (subsequently called proteobeads) are dialyzed in the presence of lipid micelles to form PLBs. As an example we employed cytochrome c oxidase from P. denitrificans with a his-tag fused to the C-terminus of subunitI...
April 5, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28388447/application-of-uv-irradiated-fe-iii-nitrilotriacetic-acid-uv-fe-iii-nta-and-uv-nta-fenton-systems-to-degrade-model-and-natural-occurring-naphthenic-acids
#8
Ying Zhang, Pamela Chelme-Ayala, Nikolaus Klamerth, Mohamed Gamal El-Din
Naphthenic acids (NAs) are a highly complex mixture of organic compounds naturally present in bitumen and identified as the primary toxic constituent of oil sands process-affected water (OSPW). This work investigated the degradation of cyclohexanoic acid (CHA), a model NA compound, and natural occurring NAs during the UV photolysis of Fe(III)-nitrilotriacetic acid (UV-Fe(III)NTA) and UV-NTA-Fenton processes. The results indicated that in the UV-Fe(III)NTA process at pH 8, the CHA removal increased with increasing NTA dose (0...
March 28, 2017: Chemosphere
https://www.readbyqxmd.com/read/28368115/generation-and-stability-of-bulk-nanobubbles
#9
Seung Hoon Oh, Jong-Min Kim
Recently, extremely small bubbles, referred to as nanobubbles, have drawn increased attention due to their novel properties and great potential for various applications. In this study, a novel method for the generation of bulk nanobubbles (BNBs) was introduced, and stability of fabricated BNBs was investigated. BNBs were created from CO2 gas with a mixing method; the chemical identity and phase state of these bubbles can be determined via infrared spectroscopy. The presence of BNBs was observed with a nanoparticle tracking analysis (NTA)...
April 3, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28350871/oxygen-tension-regulates-the-mirna-profile-and-bioactivity-of-exosomes-released-from-extravillous-trophoblast-cells-liquid-biopsies-for-monitoring-complications-of-pregnancy
#10
Grace Truong, Dominic Guanzon, Vyjayanthi Kinhal, Omar Elfeky, Andrew Lai, Sherri Longo, Zarin Nuzhat, Carlos Palma, Katherin Scholz-Romero, Ramkumar Menon, Ben W Mol, Gregory E Rice, Carlos Salomon
Our understanding of how cells communicate has undergone a paradigm shift since the recent recognition of the role of exosomes in intercellular signaling. In this study, we investigated whether oxygen tension alters the exosome release and miRNA profile from extravillous trophoblast (EVT) cells, modifying their bioactivity on endothelial cells (EC). Furthermore, we have established the exosomal miRNA profile at early gestation in women who develop pre-eclampsia (PE) and spontaneous preterm birth (SPTB). HTR-8/SVneo cells were used as an EVT model...
2017: PloS One
https://www.readbyqxmd.com/read/28338635/a-formaldehyde-sensor-based-on-molecularly-imprinted-polymer-on-a-tio%C3%A2-nanotube-array
#11
Xiaohui Tang, Jean-Pierre Raskin, Driss Lahem, Arnaud Krumpmann, André Decroly, Marc Debliquy
Today, significant attention has been brought to the development of sensitive, specific, cheap, and reliable sensors for real-time monitoring. Molecular imprinting technology is a versatile and promising technology for practical applications in many areas, particularly chemical sensors. Here, we present a chemical sensor for detecting formaldehyde, a toxic common indoor pollutant gas. Polypyrrole-based molecularly-imprinted polymer (PPy-based MIP) is employed as the sensing recognition layer and synthesized on a titanium dioxide nanotube array (TiO₂-NTA) for increasing its surface-to-volume ratio, thereby improving the sensor performance...
March 24, 2017: Sensors
https://www.readbyqxmd.com/read/28335675/development-and-application-of-an-indirect-enzyme-linked-immunosorbent-assay-using-recombinant-mag1-for-serodiagnosis-of-toxoplasma-gondii-in-dogs
#12
Xunhui Zhuo, Hongchao Sun, Zhi Zhang, Jiaqing Luo, Ying Shan, Aifang Du
Serologic tests are widely accepted and applied as means to detect anti Toxoplasma gondii immunoglobulin G antibodies(IgG). In this study, recombinant matrix antigen (MAG1) was induced by isopropyl-β-d-thiogalactoside(IPTG) and purified by nickel-nitrilotriacetic acid (Ni-NTA) purification system. We then developed and optimized an indirect enzyme-linked immunosorbent assay (ELISA) through checkerboard assays using serial dilutions of antigens and sera to assess the potential use of MAG1 in serologic detection of T...
March 23, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/28330270/expression-and-purification-of-a-gene-encoding-a-9-7%C3%A2-kda-pe-protein-of-mycobacterium-avium-subsp-paratuberculosis
#13
S Chandra Sekar, P P Goswami, R Deb
Mycobacterium avium subsp. paratuberculosis (Map) contains PE family antigens which are Proline and glutamic acid rich and may play important role as T-cell antigens. In the present study, the Map 1507 ORF encoding 9.7 kDa PE protein was amplified by polymerase chain reaction and cloned into E. coli vector pQE30 UA. The recombinant plasmid designated as pQ PE was transformed into E. coli M15 cells and induced with IPTG revealed the high level expression of 11.9 kDa His-fusion protein as estimated by migration in 15 % sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330086/cloning-expression-purification-and-characterization-of-human-mitochondrial-carbonic-anhydrase-va
#14
Danish Idrees, Sudhir Kumar, Syed Abdul Arif Rehman, Samudrala Gourinath, Asimul Islam, Faizan Ahmad, Md Imtaiyaz Hassan
Carbonic anhydrase VA (CAVA) is a mitochondrial enzyme that catalyzes the reversible hydration of CO2 to produce HCO3(-) and proton. CAV is primarily involved in several biosynthetic processes such as ureagenesis, gluconeogenesis and lipogenesis by providing bicarbonate ion. Here, we report a new strategy for cloning, expression and purification for CAVA in the bacterial system followed by its biophysical characterization. The cDNA of CAVA, a 801 nucleotide long that encodes a 267-amino acid polypeptide of molecular mass of 30-kDa (excluding signal peptide), was sub-cloned in the expression vector pET21c and transformed into Escherichia coli strain BL21 (DE3) for expression...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28317857/enhanced-biennial-variability-in-the-pacific-due-to-atlantic-capacitor-effect
#15
Lei Wang, Jin-Yi Yu, Houk Paek
The El Niño-Southern Oscillation (ENSO) and the variability in the Pacific subtropical highs (PSHs) have major impacts on social and ecological systems. Here we present an Atlantic capacitor effect mechanism to suggest that the Atlantic is a key pacemaker of the biennial variability in the Pacific including that in ENSO and the PSHs during recent decades. The 'charging' (that is, ENSO imprinting the North Tropical Atlantic (NTA) sea surface temperature (SST) via an atmospheric bridge mechanism) and 'discharging' (that is, the NTA SST triggering the following ENSO via a subtropical teleconnection mechanism) processes alternate, generating the biennial rhythmic changes in the Pacific...
March 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28315745/production-of-low-expressing-recombinant-cationic-biopolymers-with-high-purity
#16
Xuguang Chen, Alireza Nomani, Niket Patel, Arash Hatefi
The growing complexity of recombinant biopolymers for delivery of bioactive agents requires the ability to control the biomaterial structure with high degree of precision. Genetic engineering techniques have provided this opportunity to synthesize biomaterials in an organism such as E. coli with full control over their lengths and sequences. One class of such biopolymers is recombinant cationic biopolymers with applications in gene delivery, regenerative medicine and variety of other biomedical applications...
March 16, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28301074/high-throughput-inclusion-body-sizing-nano-particle-tracking-analysis
#17
Wieland N Reichelt, Andreas Kaineder, Markus Brillmann, Lukas Neutsch, Alexander Taschauer, Hans Lohninger, Christoph Herwig
The expression of pharmaceutical relevant proteins in E. coli frequently triggers inclusion body (IB) formation caused by protein aggregation. In literature, substantial effort has been invested into the quantification of IB size. But up to this date particle based methods to analyze physical IB properties of representative numbers of IBs lack sensitivity and/or lack orthogonal verification. Using high pressure freezing and automated freeze substitution for transmission electron microscopy (TEM) the cytosolic inclusion body structure was preserved within the cells...
March 16, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28296083/immobilization-of-pt-nanoparticles-via-rapid-and-reusable-electropolymerization-of-dopamine-on-tio2-nanotube-arrays-for-reversible-sers-substrates-and-nonenzymatic-glucose-sensors
#18
Jingsheng Cai, Jianying Huang, Mingzheng Ge, James Iocozzia, Zhiqun Lin, Ke-Qin Zhang, Yuekun Lai
Inspired by mussel-adhesion phenomena in nature, polydopamine (PDA) coatings are a promising route to multifunctional platforms for decorating various materials. The typical self-polymerization process of dopamine is time-consuming and the coatings of PDA are not reusable. Herein, a reusable and time-saving strategy for the electrochemical polymerization of dopamine (EPD) is reported. The PDA layer is deposited on vertically aligned TiO2 nanotube arrays (NTAs). Owing to the abundant catechol and amine groups in the PDA layer, uniform Pt nanoparticles (NPs) are deposited onto the TiO2 NTAs and can effectively prevent the recombination of electron-hole pairs generated from photo-electrocatalysis and transfer the captured electrons to participate in the photo-electrocatalytic reaction process...
March 10, 2017: Small
https://www.readbyqxmd.com/read/28294278/controlling-adsorption-and-passivation-properties-of-bovine-serum-albumin-on-silica-surfaces-by-ionic-strength-modulation-and-cross-linking
#19
Jae Hyeon Park, Tun Naw Sut, Joshua A Jackman, Abdul Rahim Ferhan, Bo Kyeong Yoon, Nam-Joon Cho
Understanding the physicochemical factors that influence protein adsorption onto solid supports holds wide relevance for fundamental insights into protein structure and function as well as for applications such as surface passivation. Ionic strength is a key parameter that influences protein adsorption, although how its modulation might be utilized to prepare well-coated protein adlayers remains to be explored. Herein, we investigated how ionic strength can be utilized to control the adsorption and passivation properties of bovine serum albumin (BSA) on silica surfaces...
March 15, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28286754/prokaryotic-expression-of-%C3%AE-13-giardin-gene-and-its-intracellular-localization-in-giardia-lamblia
#20
Xingang Yu, Auwalu Yusuf Abdullahi, Sheng Wu, Weida Pan, Xianli Shi, Wei Hu, Liping Tan, Kangxin Li, Zhen Wang, Guoqing Li
To study prokaryotic expression and subcellular localization of α-13 giardin in Giardia lamblia trophozoites, α-13 giardin gene was amplified and cloned into prokaryotic expression vector pET-28a(+). The positive recombinant plasmid was transformed into E. coli BL21(DE3) for expression by using IPTG and autoinduction expression system (ZYM-5052). The target protein was validated by SDS-PAGE and Western blotting and purified by Ni-NTA Resin. Rabbits were immunized with purified fusion proteins for preparation of polyclonal antibody; then the intracellular location of α-13 giardin was determined by fluorescence immunoassay...
2017: BioMed Research International
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