Willem S van Hoogstraten, Marit C C Lute, Zhiqiang Liu, Robin Broersen, Luca Mangili, Lieke Kros, Zhenyu Gao, Xiaolu Wang, Arn M J M van den Maagdenberg, Chris I De Zeeuw
The olivocerebellar system, which is critical for sensorimotor performance and learning, functions through modules with feedback loops. The main feedback to the inferior olive comes from the cerebellar nuclei (CN), which are predominantly GABAergic and contralateral. However, for the d subnucleus of the caudomedial accessory olive (cdMAO), a crucial region for oculomotor and upper-body movements, the source of GABAergic input has yet to be identified. Here, we demonstrate the existence of a disynaptic inhibitory projection from the medial CN (MCN) to the cdMAO via the superior colliculus (SC) by exploiting retrograde, anterograde and transsynaptic viral tracing at the light microscopic level as well as anterograde classical and viral tracing combined with immunocytochemistry at the electron microscopic level...
January 19, 2024: ENeuro