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https://www.readbyqxmd.com/read/29676528/e3-ubiquitin-ligase-rnf123-targets-lamin-b1-and-lamin-binding-proteins
#1
Richa Khanna, Vidhya Krishnamoorthy, Veena K Parnaik
Lamins are key nuclear proteins which are important for maintaining nuclear structure and function. Mutations in lamins cause a spectrum of genetic diseases termed as laminopathies. RING finger containing E3 ubiquitin ligase, RNF123 is transcriptionally upregulated in cells expressing rod-domain lamin A mutations. However, the functional relevance of RNF123 in laminopathic cells is not clear. Using a mass spectrometry based approach, we identified lamins and lamin-binding proteins retinoblastoma protein (pRb), lamina-associated polypeptide 2α (LAP2α) and emerin as RNF123-interacting proteins...
April 20, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29674003/tanshinone-iia-suppresses-fc%C3%AE%C2%B5ri-mediated-mast-cell-signaling-and-anaphylaxis-by-activation-of-the-sirt1-lkb1-ampk-pathway
#2
Xian Li, Soon Jin Park, Fansi Jin, Yifeng Deng, Ju Hye Yang, Jae-Hoon Chang, Dong-Young Kim, Jung-Ae Kim, Youn Ju Lee, Makoto Murakami, Kun Ho Son, Hyeun Wook Chang
AMP-activated protein kinase (AMPK) and its upstream mediators liver kinase B1 (LKB1) and sirtuin 1 (Sirt1) are generally known as key regulators of metabolism. We have recently reported that the AMPK pathway negatively regulates mast cell activation and anaphylaxis. Tanshinone IIA (Tan IIA), an active component of Salvia miltiorrhiza extract that is currently used for the treatment of cardiovascular and cerebrovascular diseases, shows anti-diabetic activity and improves insulin resistance in db/db mice through activation of AMPK...
April 16, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29672918/ionizing-radiation-but-not-ultraviolet-radiation-induces-mitotic-catastrophe-in-mouse-epidermal-keratinocytes-with-aberrant-cell-cycle-checkpoints
#3
Ming Wang, QingXiang Gao, Xu Teng, MeiPing Pan, TianMiao Lin, GuiXuan Zhou, BenHua Xu, ZhiCao Yue
Ultraviolet radiation (UVR) and ionizing radiation (IR) are common genotoxic stresses that damage human skin, although the specific damages to the genomic DNA are different. Here we show that in the mouse glabrous skin, both UVR and IR induce DNA damage, cell cycle arrest, and condensed cell nuclei. However, only IR induces mitotic catastrophe (MC) in the epidermis. This is because UVR induces a complete blockage of pRB phosphorylation and cell cycle arrest in the G1 phase, whereas pRB phosphorylation remains positive in a significant portion of the epidermal keratinocytes following IR exposure...
April 19, 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/29669986/quantitative-analyses-of-the-metaphase-to-anaphase-transition-reveal-differential-kinetic-regulation-for-securin-and-cyclin-b1
#4
Makoto Konishi, Norihisa Shindo, Masataka Komiya, Kozo Tanaka, Takehiko Itoh, Toru Hirota
Separation of sister chromatids is a drastic and irreversible step in the cell cycle. The key biochemistry behind this event is the proteolysis mediated by the ubiquitin ligase called the anaphase promoting complex, or APC/C. Securin and cyclin B1 are the two established substrates for APC/C whose degradation releases separase and inactivates cyclin B1-dependent kinase 1 (cdk1), respectively, at the metaphase-to-anaphase transition. In this study, we have combined biochemical quantifications with mathematical simulations to characterize the kinetic regulation of securin and cyclin B1, in the cytoplasmic and chromosomal compartments, and found that they are differentially distributed and degraded with different rates...
2018: Biomedical Research
https://www.readbyqxmd.com/read/29669919/mutations-in-the-pancreatic-secretory-enzymes-cpa1-and-cpb1-are-associated-with-pancreatic-cancer
#5
Koji Tamura, Jun Yu, Tatsuo Hata, Masaya Suenaga, Koji Shindo, Toshiya Abe, Anne MacGregor-Das, Michael Borges, Christopher L Wolfgang, Matthew J Weiss, Jin He, Marcia Irene Canto, Gloria M Petersen, Steven Gallinger, Sapna Syngal, Randall E Brand, Anil Rustgi, Sara H Olson, Elena Stoffel, Michele L Cote, George Zogopoulos, James B Potash, Fernando S Goes, Richard W McCombie, Peter P Zandi, Mehdi Pirooznia, Melissa Kramer, Jennifer Parla, James R Eshleman, Nicholas J Roberts, Ralph H Hruban, Alison Patricia Klein, Michael Goggins
To evaluate whether germline variants in genes encoding pancreatic secretory enzymes contribute to pancreatic cancer susceptibility, we sequenced the coding regions of CPB1 and other genes encoding pancreatic secretory enzymes and known pancreatitis susceptibility genes ( PRSS1 , CPA1 , CTRC , and SPINK1 ) in a hospital series of pancreatic cancer cases and controls. Variants in CPB1 , CPA1 (encoding carboxypeptidase B1 and A1), and CTRC were evaluated in a second set of cases with familial pancreatic cancer and controls...
April 18, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29666569/inhibitory-effects-of-betulinic-acid-on-lps-induced-neuroinflammation-involve-m2-microglial-polarization-via-camkk%C3%AE-dependent-ampk-activation
#6
Chuwen Li, Chao Zhang, Hefeng Zhou, Yu Feng, Fan Tang, Maggie P M Hoi, Chengwei He, Dan Ma, Chao Zhao, Simon M Y Lee
In response to the microenvironment, microglia may polarize into either an M1 pro-inflammatory phenotype, exacerbating neurotoxicity, or an M2 anti-inflammatory phenotype, conferring neuroprotection. Betulinic acid (BA) is a naturally pentacyclic triterpenoid with considerable anti-inflammatory properties. Here, we aim to investigate the potential effects of BA on microglial phenotype polarization and to reveal the underlying mechanisms of action. First, we confirmed that BA promoted M2 polarization and inhibited M1 polarization in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29663367/gadd45a-gene-silencing-by-rnai-promotes-cell-proliferation-and-inhibits-apoptosis-and-senescence-in-skin-squamous-cell-carcinoma-through-the-p53-signaling-pathway
#7
Li-Qian Liu, Fu-Jun Tian, Ying Xiong, Yan Zhao, Jian-Bo Song
Skin squamous cell carcinoma (SCC) is generally considered as nonaggressive lesions and mainly caused by ultraviolet (UV) radiation. Gadd45a is a key component protecting skin against UV-induced tumors. For that, the study aims to investigate the mechanism of Gadd45a gene silencing on cell proliferation, apoptosis, and senescence in nude mice with skin SCC through the p53 signaling pathway. Healthy nude mice was collected as the normal group and 40 nude mouse models of skin SCC were successfully established as the model group, which were sub-divided into five groups...
April 16, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29661720/pd-l1-immuno-expression-assay-in-thymomas-study-of-84-cases-and-review-of-literature
#8
Prerna Guleria, Nuzhat Husain, Saumya Shukla, Sunil Kumar, Rajinder Parshad, Deepali Jain
BACKGROUND AND AIMS: Programmed death ligand 1 (PD-L1), an immune check point inhibitor, is known to be expressed in several malignancies and is being considered as a prognostic factor and a potential immunotherapeutic target. The aim of this study was to characterize PD-L1 expression in thymomas and to determine correlation with clinicopathological features and previously published studies in the literature. METHODS: Tissue microarrays were prepared from selected blocks of thymomas and immunohistochemistry (IHC) for PD-L1 was performed...
April 4, 2018: Annals of Diagnostic Pathology
https://www.readbyqxmd.com/read/29660609/aflatoxin-b-1-contaminated-diet-disrupts-the-blood-brain-barrier-and-affects-fish-behavior-involvement-of-neurotransmitters-in-brain-synaptosomes
#9
Matheus D Baldissera, Carine F Souza, Carla Cristina Zeppenfeld, Sharine N Descovi, Karen Luise S Moreira, Maria Izabel U M da Rocha, Marcelo L da Veiga, Aleksandro S da Silva, Bernardo Baldisserotto
It is known that the cytotoxic effects of aflatoxin B1 (AFB1 ) in endothelial cells of the blood-brain barrier (BBB) are associated with behavioral dysfunction. However, the effects of a diet contaminated with AFB1 on the behavior of silver catfish remain unknown. Thus, the aim of this study was to evaluate whether an AFB1 -contaminated diet (1177 ppb kg feed-1 ) impaired silver catfish behavior, as well as whether disruption of the BBB and alteration of neurotransmitters in brain synaptosomes are involved...
April 4, 2018: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29660302/mir-223-rhob-signaling-pathway-regulates-the-proliferation-and-apoptosis-of-colon-adenocarcinoma
#10
Li-Juan Wei, Jun-An Li, Dong-Mei Bai, Ying Song
MicroRNAs (miRNAs) can function as tumor suppressor or oncogenic genes. The putative targets of miR-223 include tumor suppressor gene, RhoB. Here we sought to investigate the role of miR-223-RhoB signaling pathway in proliferation of colon cancer. We used Western blot, immunofluorescence staining, or RT-PCR to detect expression levels of miR-223 and RhoB in colon adenocarcinoma and adjacent non-cancerous tissue samples, or in human colon adenocarcinoma cell lines. MTT assay was used to determine proliferation and apoptosis in cell lines...
April 13, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29658595/huaier-suppresses-proliferative-and-metastatic-potential-of-prostate-cancer-pc3-cells-via-downregulation-of-lamin-b1-and-induction-of-autophagy
#11
Ailin Yang, Yanan Zhao, Ying Wang, Xiaojun Zha, Yunfang Zhao, Pengfei Tu, Zhongdong Hu
Prostate cancer is one of the most common malignancies occurring in males. Although large advances have been made in the pathogenesis of prostate cancer, the development of drugs with high efficacy and low toxicity for the treatment of prostate cancer is urgently needed. Recently, more and more attention has been paid to the antitumor effect of Traditional Chinese Medicine (TCM) worldwide. Trametes robiniophila Murr. (Huaier) has been applied as a type of TCM drug for ~1,600 years. Huaier exhibits excellent clinical efficacy in the treatment of cancer, including prostate cancer...
April 5, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29658576/paclitaxel-suppresses-proliferation-and-induces-apoptosis-through-regulation-of-ros-and-the-akt-mapk-signaling-pathway-in-canine-mammary-gland-tumor-cells
#12
Xiaoli Ren, Bingbing Zhao, Hongjian Chang, Min Xiao, Yuhong Wu, Yun Liu
Paclitaxel is a diterpenoid compound, derived from the pacific yew (Taxus brevifolia) berry, which exhibits antineoplastic effects against various types of cancer. However, the antitumor effects and the molecular mechanisms of paclitaxel on canine CHMm cells remain to be elucidated. The aim of the present study was to investigate the antitumor effects of paclitaxel on CHMm cells and identify relevant signal transduction pathways modulated by paclitaxel using multiple methods including MTT assay, flow cytometry, acridine orange/ethidium bromide staining, transmission electron microscopy, determination of cellular reactive oxygen species (ROS), superoxide dismutase (SOD) and malondiadehyde (MDA) and western blotting, the data indicated that paclitaxel decreased cell viability, induced G2/M‑phase cell cycle arrest, suppressed the expression of cyclin B1 and induced apoptosis in a dose‑dependent manner...
April 11, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29657412/effect-of-levamisole-vitamin-e-and-selenium-against-aflatoxicosis-in-broilers-chicken
#13
Amjed H Ulaiwi
Aim: The experiment was conducted to determine of levamisole (0.2 ml/kg-BW), Vitamin E (80 mg)+selenium (1.6 mg), and aflatoxin (B1) (positive control) compared with group without aflatoxin (negative control) on some liver enzymes (aspartate transaminase [AST] and alanine transaminase [ALT]), as well as to study the histopathological changes. Materials and Methods: The experiment included (200) 1-day-old broilers Ross 308 (Turkey source) mixed sexes. They were divided into four equal groups (50 chicks each group)...
February 2018: Veterinary World
https://www.readbyqxmd.com/read/29656006/par-4-dependent-p53-up-regulation-plays-a-critical-role-in-thymoquinone-induced-cellular-senescence-in-human-malignant-glioma-cells
#14
Karthikeyan Subburayan, Faisal Thayyullathil, Siraj Pallichankandy, Anees Rahman, Sehamuddin Galadari
Thymoquinone (TQ), the predominant bioactive constituent present in black cumin (Nigella sativa), exerts tumor suppressive activity against a wide variety of cancer cells. Cellular senescence, characterized by stable and long term loss of proliferative capacity, acts as a potent tumor suppressive mechanism. Here, we provide evidence for the first time that TQ suppresses growth of glioma cells by potentially inducing the expression of prostate apoptosis response-4 (Par-4) tumor suppressor protein. In turn, TQ-induced Par-4 expression triggers cellular senescence, as evidenced by increasing cellular size, β-galactosidase staining, G1 phase arrest, and increased expression of senescence markers such as p53, p21, Rb, and decreased expression of lamin B1, cyclin E and cyclin depended kinase-2 (CDK-2)...
April 12, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29648628/the-serosal-immune-system-of-the-thorax-in-toxicology
#15
C Frieke Kuper, Jolanda van Bilsen, Marcel V W Wijnands
The thoracic cavities receive increasing attention in toxicology, because inhaled fibers and (nano)particles can reach these cavities and challenge the local lymphoid tissues. The thoracic and abdominopelvic cavities are controlled by the serosal immune system with its special, loosely organized lymphoid clusters, namely the fat-associated lymphoid clusters (FALCs) and milky spots, which together can be denoted as serosa-associated lymphoid clusters (SALCs). These clusters house numerous innate lymphoid cells, namely the B1 and ILC2 populations...
April 10, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29645059/diagnosis-of-thymic-epithelial-tumor-subtypes-by-a-quantitative-proteomic-approach
#16
Ting Zhao, Jie Wu, Xiaohui Liu, Lei Zhang, Gang Chen, Haojie Lu
The histological typing of thymic epithelial tumours (TETs) still remains a challenge for surgical pathologists, especially when encountering borderline cases mainly focused on spindle cell types (including type A, atypical type A (aA), AB, and B3). A systematic proteomics analysis of TETs was performed using isobaric tags for relative and absolute quantification (iTRAQ) labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS). In total, 6479 and 6305 proteins were identified and quantified, respectively...
April 12, 2018: Analyst
https://www.readbyqxmd.com/read/29644433/boron-application-improves-yield-of-rice-cultivars-under-high-temperature-stress-during-vegetative-and-reproductive-stages
#17
Mohammad Shahid, Amaresh Kumar Nayak, Rahul Tripathi, Jawahar Lal Katara, Priyanka Bihari, Banwari Lal, Priyanka Gautam
It is reported that high temperatures (HT) would cause a marked decrease in world rice production. In tropical regions, high temperatures are a constraint to rice production and the most damaging effect is on spikelet sterility. Boron (B) plays a very important role in the cell wall formation, sugar translocation, and reproduction of the rice crop and could play an important role in alleviating high temperature stress. A pot culture experiment was conducted to study the effect of B application on high temperature tolerance of rice cultivars in B-deficient soil...
April 12, 2018: International Journal of Biometeorology
https://www.readbyqxmd.com/read/29628399/lkb1-controlling-quiescence-and-genomic-integrity-at-home
#18
Carmen Berasain, Matías A Ávila
The pleiotropic liver kinase B1 (LKB1) controls metabolism, cell polarity, and proliferation in an apparently cell- and context-specific manner. A recent study in Cell Reports has demonstrated that LKB1 is essential to maintain the characteristic quiescence of the liver and to secure genomic integrity during liver regeneration independently of AMPK.
April 5, 2018: Trends in Endocrinology and Metabolism: TEM
https://www.readbyqxmd.com/read/29628303/overexpression-of-tfeb-drives-a-pleiotropic-neurotrophic-effect-and-prevents-parkinson-s-disease-related-neurodegeneration
#19
Albert Torra, Annabelle Parent, Thais Cuadros, Beatriz Rodríguez-Galván, Esther Ruiz-Bronchal, Andrea Ballabio, Analía Bortolozzi, Miquel Vila, Jordi Bové
The possible implication of transcription factor EB (TFEB) as a therapeutic target in Parkinson's disease has gained momentum since it was discovered that TFEB controls lysosomal biogenesis and autophagy and that its activation might counteract lysosomal impairment and protein aggregation. However, the majority of putative direct targets of TFEB described to date is linked to a range of biological processes that are not related to the lysosomal-autophagic system. Here, we assessed the effect of overexpressing TFEB with an adeno-associated viral vector in mouse substantia nigra dopaminergic neurons...
February 27, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29627157/tgfb1-modulates-in-vitro-secretory-activity-and-viability-of-equine-luteal-cells
#20
António Galvão, Karolina Wolodko, Maria Rosa Rebordão, Dariusz Skarzynski, Graça Ferreira-Dias
In the present report we describe the involvement of transforming growth factor B1 (TGF) in functional regression and structural luteolysis in the mare. Firstly, TGF and its receptors activin-like kinase (ALK) 5 and TGF receptor 2 were identified in corpus luteum (CL) steroidogenic, endothelial and fibroblast-like cells. Also, TGF and ALK5 protein expression were shown to be increased in Mid-, and Late-CL (p < 0.05). Subsequently, using an in vitro model with Mid-CL cells, we studied the role of TGF on secretory activity and cell viability...
April 4, 2018: Cytokine
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