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Functional reconstruction

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https://www.readbyqxmd.com/read/28534477/decoding-and-reprogramming-fungal-iterative-nonribosomal-peptide-synthetases
#1
Dayu Yu, Fuchao Xu, Shuwei Zhang, Jixun Zhan
Nonribosomal peptide synthetases (NRPSs) assemble a large group of structurally and functionally diverse natural products. While the iterative catalytic mechanism of bacterial NRPSs is known, it remains unclear how fungal NRPSs create products of desired length. Here we show that fungal iterative NRPSs adopt an alternate incorporation strategy. Beauvericin and bassianolide synthetases have the same C1-A1-T1-C2-A2-MT-T2a-T2b-C3 domain organization. During catalysis, C3 and C2 take turns to incorporate the two biosynthetic precursors into the growing depsipeptide chain that swings between T1 and T2a/T2b with C3 cyclizing the chain when it reaches the full length...
May 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/28534087/interspecies-differences-in-pth-mediated-pka-phosphorylation-of-the-epithelial-calcium-channel-trpv5
#2
Mark K van Goor, Sjoerd Verkaart, Teunis J van Dam, Martijn A Huynen, Jenny van der Wijst
The epithelial calcium (Ca(2+)) channel TRPV5 (transient receptor potential vanilloid 5) is expressed in the distal convoluted tubule of the kidney and facilitates active Ca(2+) reabsorption. This process is instrumental for the maintenance of Ca(2+) homeostasis. Therefore, all aspects of TRPV5 function are tightly regulated by the calciotropic parathyroid hormone (PTH). Rabbit (rb)TRPV5 channel activity was shown to be stimulated upon PTH-mediated protein kinase A (PKA) phosphorylation. Since there is incomplete conservation of the PKA consensus motif (RR/QxT) across species, the aim of this study was to extend these findings to humans and characterize the expression and function of human (h)TRPV5...
May 22, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28532969/lip-reconstruction-using-a-functioning-serratus-anterior-free-flap-preliminary-study
#3
A O Gundeslioglu, E C Karadag, I Inan, L Jasharllari, M N Selimoglu, F Guney, B Yuruten, M Bekerecioglu
Reconstructive surgery to the lips requires the replacement of defective tissues with similarly functioning tissues. While non-dynamic free tissue transfers provide adequate lower lip reconstruction, improved benefits may be obtained with innervated free muscle flaps. This study reports the use of innervated serratus anterior muscle free flaps for lip reconstruction in five patients. All patients had squamous cell carcinoma of either the upper or lower lip. After resection of the tumours, the resultant defects comprised two-thirds of the lips...
May 19, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28532890/fascial-manipulation-%C3%A2-for-persistent-knee-pain-following-acl-and-meniscus-repair
#4
Sannasi Rajasekar, Aurélie Marie Marchand
Fascial Manipulation(®) (FM) is a manual therapy approach for the treatment of musculoskeletal pain. Anomalous fascial tension is common following surgery due to surgical scar, inadequate mobility and fear of movement. Fascial tension may result in pain and loss of mobility. This case report aims at investigating the effectiveness of FM(®) on pain and function in a patient following knee surgery. A 32 years old male patient, with persisting knee pain following anterior cruciate ligament reconstruction (hamstring graft) and meniscal repair, underwent the systematic FM(®) assessment process, the selected centers of coordination of myofascial units were treated...
April 2017: Journal of Bodywork and Movement Therapies
https://www.readbyqxmd.com/read/28532493/distal-femoral-replacement-with-the-mml-system-a-single-center-experience-with-an-average-follow-up-of-86%C3%A2-months
#5
Andreas Toepfer, Norbert Harrasser, Paul-Ruben Schwarz, Florian Pohlig, Ulrich Lenze, Heinrich M L Mühlhofer, Ludger Gerdesmeyer, Ruediger von Eisenhart-Rothe, Christian Suren
BACKGROUND: The aim of this study was to compare the functional outcomes and complication rates after distal femoral replacement (DFR) performed with the modular Munich-Luebeck (MML) modular prosthesis (ESKA/Orthodynamics, Luebeck, Germany) in patients being treated for malignant disease or failed total knee arthroplasty. METHODS: A retrospective review of patient charts and a functional investigation (involving Musculoskeletal Tumor Society Score [MSTS], American Knee Society Score [AKSS], Oxford Knee Score [OKS], Western Ontario and McMaster Universities Osteoarthritis Index [WOMAC], Toronto Extremity Salvage Score [TESS], the 12-Item Short-Form [SF-12] Health Survey, and a failure classification system developed by Henderson et al...
May 22, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28532442/an-integrated-enhancement-and-reconstruction-strategy-for-the-quantitative-extraction-of-actin-stress-fibers-from-fluorescence-micrographs
#6
Zhen Zhang, Shumin Xia, Pakorn Kanchanawong
BACKGROUND: The stress fibers are prominent organization of actin filaments that perform important functions in cellular processes such as migration, polarization, and traction force generation, and whose collective organization reflects the physiological and mechanical activities of the cells. Easily visualized by fluorescence microscopy, the stress fibers are widely used as qualitative descriptors of cell phenotypes. However, due to the complexity of the stress fibers and the presence of other actin-containing cellular features, images of stress fibers are relatively challenging to quantitatively analyze using previously developed approaches, requiring significant user intervention...
May 22, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28532119/poly-d-l-lactide-polycaprolactone-bioactive-glasss-nanocomposites-materials-for-anterior-cruciate-ligament-reconstruction-screws-the-effect-of-glass-surface-functionalization-on-mechanical-properties-and-cell-behaviors
#7
Javad Esmaeilzadeh, Saeed Hesaraki, Seyed Mohammad-Mehdi Hadavi, Mohammad Hosein Ebrahimzadeh, Masoud Esfandeh
In this paper, different nanocomposites made of a polymer blend (80% of PDLLA and 20% of PCL in w/w) and various amounts of a sol-gel derived bioactive glass nanoparticles (0, 1, 3 and 6wt%) were prepared using a solvent-evaporation technique. The morphology, mechanical properties and osteoblastic cell behaviors of the nanocomposites were evaluated. According to the early results, addition of bioactive glass nanoparticles to the polymer matrix reduced the tensile and flexural strength because of a non-uniform distribution of the nanoparticles...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28531346/projector-augmented-wave-method-incorporated-into-gauss-type-atomic-orbital-based-density-functional-theory
#8
Xiao-Gen Xiong, Takeshi Yanai
The Projector Augmented Wave (PAW) method developed by Blochl is well recognized as an efficient, accurate pseudopotential approach in solid-state density functional theory (DFT) calculations with plane-wave basis. Here we present an approach to incorporate the PAW method into the Gauss-type function (GTF) based DFT implementation, which is widely used for molecular quantum chemistry calculations. The nodal and high-exponent GTF components of valence molecular orbitals (MOs) are removed or pseudized by the ultrasoft PAW treatment, while there is elaborate transparency to construct accurate and well-controlled pseudopotential from all-electron atomic description and to reconstruct all-electron form of valence MOs from the pseudo MOs...
May 22, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28530904/wiring-variations-that-enable-and-constrain-neural-computation-in-a-sensory-microcircuit
#9
William F Tobin, Rachel I Wilson, Wei-Chung Allen Lee
Neural network function can be shaped by varying the strength of synaptic connections. One way to achieve this is to vary connection structure. To investigate how structural variation among synaptic connections might affect neural computation, we examined primary afferent connections in the Drosophila olfactory system. We used large-scale serial section electron microscopy to reconstruct all the olfactory receptor neuron (ORN) axons that target a left-right pair of glomeruli, as well as all the projection neurons (PNs) postsynaptic to these ORNs...
May 22, 2017: ELife
https://www.readbyqxmd.com/read/28530621/-laparoscopic-ureteral-reimplantation-in-the-treatment-of-urinary-tract-complications-after-renal-transplantation
#10
Juan Soto Villalba, Jesús Rosety Rodríguez, Cristina León Delgado, María José Ledo Cepero, Patricia Parra Serván, José Luis Álvarez-Ossorio Fernández
OBJECTIVES: Laparoscopic ureteral reconstructive surgery is routinely performed , because it demonstrated efficacy and safety profiles similar to open surgery in expert hands. The most frequent surgical complications after transplant are urological, appearing in up to 12,5% of the cases; they can compromise graft function and mortality. The most frequent ones include ureterovesical anastomosis stenosis (2,5-7,5%) and vesicoureteral reflux (0,4-2,2), which present in up to 80% of the cases...
May 2017: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28530619/-current-status-of-laparoscopic-sacrocolpopexy-in-the-treatment-of-pelvic-organ-prolapse
#11
Carmen González-Enguita, Natalia Gennaro-DellaRossa, Esther López-López, Juliana Escobar-Castaño, Percy Miguel Rodríguez-Castro, Raquel González-López
OBJECTIVES: Surgical restoration of pelvic floor anatomy in pelvic organ prolapse (POP) should avoid extensive areas that may injure healthy tissues and lead to scar fibrosis producing dysfunctional rigidity. Laparoscopic sacrocolpopexy corrects POP by lifting pelvic elements with a minimally invasive procedure.Various current strategies and approaches make it a diverse procedure. METHODS: We performed a bibliographic review on the published experience about abdominal sacrocolpopexy over the last 20 years...
May 2017: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28530617/-principles-of-urinary-tract-reconstructive-surgery
#12
Juan Gomez Rivas, Jesús Cisneros Ledo
INTRODUCTION: In the last 15 years, the role of laparoscopic surgery has progressively increased to include reconstructive procedures such as pyeloplasty and management of ureteral stricture, in addition to being a field in continuous development, this refers to the improvement of ergonomics, new instruments and new techniques. We present a review on basic features of laparoscopic reconstructive surgery in urology. ACQUISITION OF EVIDENCE: For this review, an exhaustive literature search was performed in PUBMED, MEDILNE, BioMed central and others, with the keywords: reconstructive surgery, urology, laparoscopy...
May 2017: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28529417/nasolabial-perforator-flap-for-one-stage-reconstruction-of-nasal-defects
#13
Siddharth Prakash, Ritesh Panda, Vivek Kumar, Shiv Shankar Saha, Lalit Choudhary, Anurag Pandey, J Sasidhar Reddy
BACKGROUND: The excellent freedom of movement and range of this flap when based on a the nasolabial perforator flap have not been sufficiently explored. In this study, along with demonstrating the other key advantages of this flap over its traditional counterpart, we will endeavour to fill these lacunae in the available literature. MATERIALS AND METHODS: From February 2009 to February 2012, twenty patients with nasal defects were repaired with a nasolabial perforator flap in the Department of Plastic and Cosmetic Surgery at Sir Ganga Ram Hospital, New Delhi...
January 2017: Journal of Cutaneous and Aesthetic Surgery
https://www.readbyqxmd.com/read/28529251/image-quality-and-clinical-usefulness-of-ray-summation-image-reconstructed-from-ct-data-compared-with-digital-radiography
#14
Shogo Suzuki, Katsuhiro Ichikawa, Shigeru Tamaki
Ray-summation (raysum) images reconstructed from computed tomography (CT) volume data resemble digital radiography (DR) images. Therefore, they have a potential to be used instead of DR images.The aim of this study was to compare the physical image quality evaluated by signal-difference-to-noise ratio (SDNR) and clinical usefulness between raysum and DR images. We employed an oval water phantom simulating adult abdomen for image quality measurement. Raysum images were reconstructed from CT volume data using an assumed x-ray quality of 70 keV...
2017: Nihon Hoshasen Gijutsu Gakkai Zasshi
https://www.readbyqxmd.com/read/28529013/bipolar-fresh-total-osteochondral-allograft-in-the-ankle-is-it-a-successful-long-term-solution
#15
S Giannini, A Mazzotti, F Vannini
INTRODUCTION: Severe post-traumatic ankle arthritis poses a reconstructive challenge in young and active patients. Although technically demanding and despite unsolved immunological issues, bipolar fresh total osteochondral allograft (BFTOA) represent an intriguing option to arthrodesis and prosthetic replacement. The purpose of this paper is to evaluate the outcomes of a series of 48 ankle BFTOA at 10 years follow up and to investigate the rate of survival long term. METHODS: 58 patients underwent BFTOA, of these 48 were available for follow up...
May 11, 2017: Injury
https://www.readbyqxmd.com/read/28528636/correlative-two-photon-and-serial-block-face-scanning-electron-microscopy-in-neuronal-tissue-using-3d-near-infrared-branding-maps
#16
Robert M Lees, Christopher J Peddie, Lucy M Collinson, Michael C Ashby, Paul Verkade
Linking cellular structure and function has always been a key goal of microscopy, but obtaining high resolution spatial and temporal information from the same specimen is a fundamental challenge. Two-photon (2P) microscopy allows imaging deep inside intact tissue, bringing great insight into the structural and functional dynamics of cells in their physiological environment. At the nanoscale, the complex ultrastructure of a cell's environment in tissue can be reconstructed in three dimensions (3D) using serial block face scanning electron microscopy (SBF-SEM)...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28528191/subcutaneous-implant-breast-reconstruction-time-to-reconsider
#17
REVIEW
M-K Tasoulis, F M Iqbal, S Cawthorn, F MacNeill, R Vidya
Improvements in breast surgery techniques such as skin and nipple preserving mastectomy and innovative prosthetics (implants, acellular dermal matrices and meshes) is renewing interest in subcutaneous (pre-pectoral) implant reconstruction. The aim of this paper is to review the current literature in an attempt to provide a rationale that may support a return to subcutaneous implant placement, so minimising the pain and functional problems resulting from submuscular breast reconstruction.
May 4, 2017: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28527883/endovascular-iliocaval-stent-reconstruction-for-inferior-vena-cava-filter-associated-iliocaval-thrombosis-approach-technical-success-safety-and-two-year-outcomes-in-120-patients
#18
Jeffrey Forris Beecham Chick, Alexandria Jo, J Matthew Meadows, Steven D Abramowitz, Minhaj S Khaja, Kyle J Cooper, David M Williams
PURPOSE: To report technical success, outcomes, and patency of iliocaval stent reconstruction for inferior vena cava (IVC) filter-bearing iliocaval thrombosis. MATERIALS AND METHODS: A total of 120 patients with 123 IVC filters and symptomatic iliocaval thrombosis underwent stent reconstruction. Mean patient age was 55 years (range, 19-88 y). Filters included 70 (57%) retrievable and 53 (43%) permanent filters. Symptoms included lower extremity swelling or pain (n = 93), ulcers (n = 8), phlegmasia (n = 7), back pain (n = 5), shortness of breath (n = 4), worsening renal function (n = 2), and stenosis identified during translumbar catheter placement (n = 1)...
May 18, 2017: Journal of Vascular and Interventional Radiology: JVIR
https://www.readbyqxmd.com/read/28527321/short-term-exposure-to-gold-nanoparticle-suspension-impairs-swimming-behavior-in-a-widespread-calanoid-copepod
#19
François-Gaël Michalec, Markus Holzner, Alexandre Barras, Anne-Sophie Lacoste, Loïc Brunet, Jae-Seong Lee, Christian Slomianny, Rabah Boukherroub, Sami Souissi
Calanoid copepods play an important role in the functioning of marine and brackish ecosystems. Information is scarce on the behavioral toxicity of engineered nanoparticles to these abundant planktonic organisms. We assessed the effects of short-term exposure to nonfunctionalized gold nanoparticles on the swimming behavior of the widespread estuarine copepod Eurytemora affinis. By means of three-dimensional particle tracking velocimetry, we reconstructed the trajectories of males, ovigerous and non-ovigerous females...
May 17, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28526903/compartmental-tongue-resection-with-submental-island-flap-reconstruction-for-large-carcinoma-of-the-oral-tongue
#20
Sameh Roshdy, Mohamed Elbadrawy, Ashraf Khater, Tamer Fady, Hend A El-Hadaad, Nazem Shams, Hany M Elbarbary
BACKGROUND: Tongue resection is a surgical challenge because of its adverse effects on language articulation, swallowing, respiration, the eventual quality of life, and poor prognosis of advanced disease. To date, the currently accepted standard treatment has been based on excision of the primary lesion with a 1.5-2-cm circumferential macroscopic margin. Compartmental tongue surgery (CTS) is a surgical technique that removes an anatomo-functional compartment containing the primary tumor...
May 19, 2017: Oral and Maxillofacial Surgery
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