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Tethering complex

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https://www.readbyqxmd.com/read/29166001/a-redox-role-for-the-4fe4s-cluster-of-yeast-dna-polymerase-%C3%AE
#1
Phillip L Bartels, Joseph L Stodola, Peter M J Burgers, Jacqueline K Barton
A [4Fe4S]2+ cluster in the C-terminal domain of the catalytic subunit of the eukaryotic B-family DNA polymerases is essential for the formation of active multi-subunit complexes. Here we use a combination of electrochemical and biochemical methods to assess the redox activity of the [4Fe4S]2+ cluster in Saccharomyces cerevisiae polymerase (Pol) δ, the lagging strand DNA polymerase. We find that Pol δ bound to DNA is indeed redox-active at physiological potentials, generating a DNA-mediated signal electrochemically with a midpoint potential of 113 ± 5 mV versus NHE...
November 22, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29165989/bispentafluorophenyl-containing-additive-enhancing-efficiency-and-morphological-stability-of-polymer-solar-cells-via-hand-grabbing-like-supramolecular-pentafluorophenyl-fullerene-interactions
#2
Kai-En Hung, Che-En Tsai, Shao-Ling Chang, Yu-Ying Lai, U-Ser Jeng, Fong-Yi Cao, Chain-Shu Hsu, Chun-Jen Su, Yen-Ju Cheng
A new class of additive materials bis(pentafluorophenyl) diesters (BFEs) where the two pentafluorophenyl (C6F5) moieties are attached at the both ends of a linear aliphatic chain with tunable tether lengths (BF5, BF7, and BF13) were designed and synthesized. In the presence of the BF7 to restrict the migration of fullerene by hand-grabbing-like supramolecular interactions induced between the C6F5 groups and the surface of fullerene, the P3HT:PC61BM:BF7 device showed stable device characteristics after thermal heating at 150 oC for 25 h...
November 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29160806/synthesis-of-nitrogen-heterocycles-using-samarium-ii-iodide
#3
REVIEW
Shicheng Shi, Michal Szostak
Nitrogen heterocycles represent vital structural motifs in biologically-active natural products and pharmaceuticals. As a result, the development of new, convenient and more efficient processes to N-heterocycles is of great interest to synthetic chemists. Samarium(II) iodide (SmI₂, Kagan's reagent) has been widely used to forge challenging C-C bonds through reductive coupling reactions. Historically, the use of SmI₂ in organic synthesis has been focused on the construction of carbocycles and oxygen-containing motifs...
November 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29160601/metalated-chromene-and-chromone-complexes-ph-switchable-metal-carbon-bonding-interaction-photo-triggerable-chromone-delivery-application-and-antioxidative-activity
#4
Sze-Wing Ng, Lai-Hon Chung, Chi-Fung Yeung, Hoi-Shing Lo, Hau-Lam Shek, Tian-Shu Kang, Chung-Hang Leung, Chun-Yuen Wong
The two families of Ru(II)-chromene and -chromone complexes isolated in this work represent the first examples of metalated chromene and chromone complexes synthesized through transition-metal mediated cyclization of phenol-tethered ynone. These unprecedented metalated heterocyclic compounds exhibit remarkable features, such as pH-switchable metal-carbon bonding interactions, photo-triggerable release of organic chromone upon visible-light irradiation, and superior antioxidative property to their organic analog (1,4-benzopyrone)...
November 21, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29158815/tethering-interleukin-22-to-apolipoprotein-a-i-ameliorates-mice-from-acetaminophen-induced-liver-injury
#5
Wei Chen, Xuyao Zhang, Jiajun Fan, Wenjing Zai, Jingyun Luan, Yubin Li, Shaofei Wang, Qicheng Chen, Yichen Wang, Yanxu Liang, Dianwen Ju
Increasing evidence indicates that interleukin-22 (IL-22) holds tremendous potential as a protective agent in preventing liver injury, but its pleiotropic effects and pathogenic role in carcinogenesis, rheumatoid arthritis and psoriasis restrict its systemic application. Here, we first developed a nanoparticle (liposIA) as a liver-targeted agent through IL-22 tethered to apolipoprotein A-I (ApoA-I) in a gene therapy vector. LiposIA was prepared using thin film dispersion method and the complexes exhibited desirable nanoparticle size, fine polydisperse index, highly efficient transfection, and excellent serum and storage stability...
2017: Theranostics
https://www.readbyqxmd.com/read/29158426/cohesin-function-in-cohesion-condensation-and-dna-repair-is-regulated-by-wpl1p-via-a-common-mechanism-in-saccharomyces%C3%A2-cerevisiae
#6
Michelle S Bloom, Douglas Koshland, Vincent Guacci
Cohesin tethers DNA to mediate sister chromatid cohesion, chromosome condensation, and DNA repair. How the cell regulates cohesin to perform these distinct functions remains to be elucidated. One cohesin regulator, Wpl1p, was characterized in Saccharomyces cerevisiae as a promoter of efficient cohesion and an inhibitor of condensation. Wpl1p is also required for resistance to DNA damaging agents. Here we provide evidence that Wpl1p promotes the timely repair of DNA damage induced during S-phase. Previous studies indicate that Wpl1p destabilizes cohesin's binding to DNA by modulating the interface between the cohesin subunits Mcd1p and Smc3p...
November 20, 2017: Genetics
https://www.readbyqxmd.com/read/29157516/anatomy-of-the-tricuspid-valve-pathophysiology-of-functional-tricuspid-regurgitation-and-implications-for-percutaneous-therapies
#7
REVIEW
Nicola Buzzatti, Michele De Bonis, Neil Moat
The tricuspid valve is a complex dynamic apparatus made up of many different closely linked structures: the annulus, the three leaflets, the chordae, the papillary muscles and the right ventricle. Other nearby structures, such as the coronary sinus ostium, the conduction system, the membranous septum, and the right coronary artery must be taken into account when dealing with the tricuspid. Annulus dilation and leaflet tethering due to right ventricular remodeling are the 2 major mechanisms responsible for most tricuspid regurgitation cases...
January 2018: Interventional cardiology clinics
https://www.readbyqxmd.com/read/29155589/the-reaction-mechanism-of-isopentenyl-phosphate-kinase-a-qm-mm-study
#8
James McClory, David J Timson, Warispreet Singh, Jian Zhang, Meilan Huang
Isopentenyl phosphate kinase (IPK) catalyzes the Mg2+-ATP dependent phosphorylation reactions to produce isopentenyl diphosphate, an important precursor in the synthesis of isopentenols. However, the position of the divalent metal ion in the crystal structures of IPK in complex with ATP and its native substrate IP has not been definitively resolved, and as a result ambiguity surrounds the catalytic mechanism of IP, limiting its exploitation as a biofuel and in drug design. Here we report the conformational change caused by substrate binding and the catalytically competent structure in complex with the metal ion Mg2+ using molecular dynamic simulations and density functional theory-based quantum mechanics/molecular mechanics calculations (B97d/AMBER99)...
November 20, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29149603/structural-basis-of-mitochondrial-transcription-initiation
#9
Hauke S Hillen, Yaroslav I Morozov, Azadeh Sarfallah, Dmitry Temiakov, Patrick Cramer
Transcription in human mitochondria is driven by a single-subunit, factor-dependent RNA polymerase (mtRNAP). Despite its critical role in both expression and replication of the mitochondrial genome, transcription initiation by mtRNAP remains poorly understood. Here, we report crystal structures of human mitochondrial transcription initiation complexes assembled on both light and heavy strand promoters. The structures reveal how transcription factors TFAM and TFB2M assist mtRNAP to achieve promoter-dependent initiation...
November 16, 2017: Cell
https://www.readbyqxmd.com/read/29148789/amphiphilic-n-heterocyclic-carbene-stabilized-gold-nanoparticles-and-their-self-assembly-in-polar-solvents
#10
Mina R Narouz, Chien-Hung Li, Ali Nazemi, Cathleen M Crudden
Soft matter-directed self-assembly of amphiphilic inorganic nanoparticles (NPs) has recently emerged as a promising approach to access NP ensembles with superior collective properties. While thiol-terminated molecules are primarily employed to tether the amphiphilic ligand to the metal, serious concerns remain regarding the stabilities of the resulting NPs and their corresponding superstructures. As an alternative to such ligands, we report the first example of amphiphilic N-heterocyclic carbene (NHC)-functionalized gold nanoparticles (AuNPs)...
November 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29141912/wh2-and-proline-rich-domains-of-wasp-family-proteins-collaborate-to-accelerate-actin-filament-elongation
#11
Peter Bieling, Scott D Hansen, Orkun Akin, Tai-De Li, Carl C Hayden, Daniel A Fletcher, R Dyche Mullins
WASP-family proteins are known to promote assembly of branched actin networks by stimulating the filament-nucleating activity of the Arp2/3 complex. Here, we show that WASP-family proteins also function as polymerases that accelerate elongation of uncapped actin filaments. When clustered on a surface, WASP-family proteins can drive branched actin networks to grow much faster than they could by direct incorporation of soluble monomers. This polymerase activity arises from the coordinated action of two regulatory sequences: (i) a WASP homology 2 (WH2) domain that binds actin, and (ii) a proline-rich sequence that binds profilin-actin complexes...
November 15, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29135058/distinct-features-of-multivesicular-body-lysosome-fusion-revealed-by-a-new-cell-free-content-mixing-assay
#12
Mahmoud Abdul Karim, Dieter Ronny Samyn, Sevan Mattie, Christopher Leonard Brett
When marked for degradation, surface receptor and transporter proteins are internalized and delivered to endosomes where they are packaged into intralumenal vesicles (ILVs). Many rounds of ILV formation create multivesicular bodies (MVBs) that fuse with lysosomes exposing ILVs to hydrolases for catabolism. Despite being critical for protein degradation, the molecular underpinnings of MVB-lysosome fusion remain unclear, although machinery underlying other lysosome fusion events is implicated. But how then is specificity conferred? And how is MVB maturation and fusion coordinated for efficient protein degradation? To address these questions, we developed a cell-free MVB-lysosome fusion assay using S...
November 14, 2017: Traffic
https://www.readbyqxmd.com/read/29134801/adsorbed-polyzwitterion-copolymer-layers-designed-for-protein-repellency-and-interfacial-retention
#13
S Kalasin, R A Letteri, T Emrick, M M Santore
Poly(2-methacryloyloxyethyl phosphorylcholine) (pMPC), when end-tethered to surfaces by the adsorption of copolymeric cationic segments, forms adsorbed layers that substantially reduce protein adsorption. This study examined variations in the molecular architecture of copolymers containing cationic poly(trimethylammonium ethyl methacrylate (pTMAEMA) anchor blocks that adsorbed strongly to negative surfaces. With appropriate copolymer design, the pTMAEMA blocks were shielded, by pMPC tethers, from solution-phase proteins...
November 14, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29132559/oncoplastic-breast-reconstruction-should-all-patients-be-considered
#14
REVIEW
Mehran Habibi, Kristen P Broderick, Mohamad E Sebai, Lisa K Jacobs
Oncoplastic surgery of the tissue defect from partial mastectomy should be considered for all patients. It can result in in significant asymmetries from scar contraction, skin tethering, and alterations in the nipple areolar complex location. Indications, risks, and benefits are discussed. Optimal procedures are described, considering resected specimen volume, primary tumor location, tumor to breast size ratio, and the impact on the nipple areolar complex. Indications for plastic surgery consultation and joint surgery are discussed...
January 2018: Surgical Oncology Clinics of North America
https://www.readbyqxmd.com/read/29129923/ist1-regulates-escrt-iii-assembly-and-function-during-multivesicular-endosome-biogenesis-in-caenorhabditis-elegans-embryos
#15
E B Frankel, Raakhee Shankar, James J Moresco, John R Yates, Niels Volkmann, Anjon Audhya
Degradation of most integral membrane proteins is directed by the endosomal sorting complex required for transport (ESCRT) machinery, which selectively targets ubiquitin-modified cargoes into intralumenal vesicles (ILVs) within multivesicular endosomes (MVEs). To better understand the mechanisms underlying ESCRT-mediated formation of ILVs, we exploited the rapid, de novo biogenesis of MVEs during the oocyte-to-embryo transition in C. elegans. In contrast to previous models suggesting that ILVs form individually, we demonstrate that they remain tethered to one another subsequent to internalization, arguing that they bud continuously from stable subdomains...
November 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/29129639/determinants-of-histone-h3k4-methylation-patterns
#16
Luis M Soares, P Cody He, Yujin Chun, Hyunsuk Suh, TaeSoo Kim, Stephen Buratowski
Various factors differentially recognize trimethylated histone H3 lysine 4 (H3K4me3) near promoters, H3K4me2 just downstream, and promoter-distal H3K4me1 to modulate gene expression. This methylation "gradient" is thought to result from preferential binding of the H3K4 methyltransferase Set1/complex associated with Set1 (COMPASS) to promoter-proximal RNA polymerase II. However, other studies have suggested that location-specific cues allosterically activate Set1. Chromatin immunoprecipitation sequencing (ChIP-seq) experiments show that H3K4 methylation patterns on active genes are not universal or fixed and change in response to both transcription elongation rate and frequency as well as reduced COMPASS activity...
November 16, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29125732/targeted-activation-of-molecular-transportation-by-visible-light
#17
Ammathnadu S Amrutha, K R Sunil Kumar, Takashi Kikukawa, Nobuyuki Tamaoki
Regulated transportation of nanoscale objects with a high degree of spatiotemporal precision is a prerequisite for the development of targeted molecular delivery. In vitro integration of the kinesin-microtubule motor system with synthetic molecules offers opportunities to develop controllable molecular shuttles for lab-on-a-chip applications. We attempted a combination of the kinesin-microtubule motor system with push-pull type azobenzene tethered inhibitory peptides (azo-peptides) through which reversible, spatiotemporal control over the kinesin motor activity was achieved locally by a single, visible wavelength...
November 16, 2017: ACS Nano
https://www.readbyqxmd.com/read/29125278/grafting-commercial-surfactants-brij-ciej-and-peg-to-electrodes-via-aryldiazonium-salts
#18
Ophélie Squillace, Charles Esnault, Jean-François Pilard, Guillaume Brotons
Grafting commercial surfactants appears to be a simple way to modify electrodes and conducting interfaces, avoiding the synthesis of complex organic molecules. A new surface functionalisation route is presented to build surfactants coatings with monolayer thicknesses grafting molecules considered as non-reactive. A monolayer of -SO2Cl functions (from a para-benzene sulfonyl chloride) was first electro-grafted. It shown a high reactivity toward weak nucleophiles commonly found on surfactants end-moieties such as hydroxyl groups (-OH) and it was used to covalently graft: 1) non-ionic diblock oligomers (Brij or CiEj, CxH2x+1(OCH2CH2)nOH with x=16 and n=23 for Brij58; x=16 and n=10 for Brij C10; x=16 and n=2 for Brij52); 2) Poly (ethylene glycol) PEG short chains (PEO9 for (OCH2CH2)nOH with n=9); and mixed formula...
November 10, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29125140/mouse-embryonic-stem-cells-have-increased-capacity-for-replication-fork-restart-driven-by-the-specific-filia-floped-protein-complex
#19
Bo Zhao, Weidao Zhang, Yixian Cun, Jingzheng Li, Yan Liu, Jing Gao, Hongwen Zhu, Hu Zhou, Rugang Zhang, Ping Zheng
Pluripotent stem cells (PSCs) harbor constitutive DNA replication stress during their rapid proliferation and the consequent genome instability hampers their applications in regenerative medicine. It is therefore important to understand the regulatory mechanisms of replication stress response in PSCs. Here, we report that mouse embryonic stem cells (ESCs) are superior to differentiated cells in resolving replication stress. Specifically, ESCs utilize a unique Filia-Floped protein complex-dependent mechanism to efficiently promote the restart of stalled replication forks, therefore maintaining genomic stability...
November 10, 2017: Cell Research
https://www.readbyqxmd.com/read/29124827/recovery-from-temporary-er-stress-in-plants-relies-on-tissue-specific-and-largely-independent-roles-of-bzip28-and-bzip60-as-well-as-an-antagonizing-function-of-bax-inhibitor1-onto-the-pro-adaptive-signaling-mediated-by-bzip28
#20
Cristina Ruberti, YaShiuan Lai, Federica Brandizzi
The unfolded protein response (UPR) is an ancient signaling pathway that commits to life-or-death outcomes in response to proteotoxic stress in the endoplasmic reticulum (ER). In plants, the membrane-tethered transcription factor bZIP28 and the ribonuclease-kinase IRE1 along with its splicing target, bZIP60, govern the two cytoprotective UPR signaling pathways known to date. The conserved ER membrane-associated BAX inhibitor1 (BI1) modulates ER stress-induced programmed cell death through yet-unknown mechanisms...
November 10, 2017: Plant Journal: for Cell and Molecular Biology
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