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Tethering complex

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https://www.readbyqxmd.com/read/29330305/restricted-processing-of-cd16a-fc-%C3%AE-receptor-iiia-n-glycans-from%C3%A2-primary-human-nk-cells-impacts-structure-and-function
#1
Kashyap R Patel, Jacob T Roberts, Ganesh P Subedi, Adam W Barb
CD16a/Fc γ receptor IIIa is the most abundant antibody Fc receptor expressed on human natural killer(NK) cells and activates a protective cytotoxic response following engagement with antibody clustered on the surface of a pathogen or diseased tissue. Therapeutic monoclonal antibodies(mAbs) with greater Fc-mediated affinity for CD16a show superior therapeutic outcome, however, one significant factor that promotes antibody-CD16a interactions, the asparagine-linked carbohydrates(N-glycans), remains undefined...
January 12, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29319811/identification-of-a-consensus-motif-in-erg28p-required-for-c-4-demethylation-in-yeast-ergosterol-biosynthesis-based-on-mutation-analysis
#2
Xia Ke, Xiao-Yuan Xia, Ren-Chao Zheng, Yu-Guo Zheng
The Erg28p protein is localized to the endoplasmic reticulum (ER), where it acts as a scaffold to tether the C-4 demethylase complex involved in the sterol biosynthesis pathway of Saccharomyces cerevisiae. However, due to the challenges involved in characterizing the interactions of membrane proteins, the precise region of Erg28p that is responsible for the assembly of this enzyme complex remains unknown. To address this question, mutants with serial truncations in the C-terminus of Erg28p were constructed based on a topology prediction of its transmembrane domain...
January 8, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29319703/tailoring-2d-and-3d-molecular-sieves-structures-for-polyolefin-composites-do-all-roads-lead-to-remarkable-performances
#3
H M Moura, N L Gibbons, S A Miller, H O Pastore
Multiple synthetic strategies were performed in order to tether a zirconium-based catalyst to the 2D and 3D molecular sieves for olefin polymerizations. The anchoring of fluorene silane to the mesoporous MCM-41 was performed in order to obtain a stable catalyst for olefin polymerization (1@MCM-41). Using spectroscopic methods, this system was shown to have the metal center locked on a face down conformation with the surface. Also, immobilized zirconium complexes have been prepared on three different types of aminopropyl-modified supports (2@magadiite, 2@MCM-41 and 3@MCM-48)...
January 10, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29318318/synthesis-and-x-ray-structural-analysis-of-the-ruthenium-iii-complex-na-trans-rucl4-dmso-pyrdiaz-the-diazene-derivative-of-antitumor-nami-pyr
#4
Jure Vajs, Andrej Pevec, Martin Gazvoda, Damijana Urankar, Evgeny Goreshnik, Slovenko Polanc, Janez Košmrlj
Novel tetrachloridoruthenium(III) complex Na[trans-RuCl4(DMSO)(PyrDiaz)] (3) with pyridine-tethered diazenedicarboxamide PyrDiaz ligand (PyrDiaz = N1-(4-isopropylphenyl)-N2-(pyridin-2-ylmethyl)diazene-1,2-dicarboxamide) was synthesized by direct coupling of PyrDiaz with sodium trans-bis(dimethyl sulfoxide)tetrachloridoruthenate(III) (Na-[trans-Ru(DMSO)2Cl4]). Compound 3 is the analogue of the antimetastatic Ru(III) complex NAMI-A and NAMI-Pyr. Single crystal X.
December 2017: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/29315910/early-late-heterobimetallic-tantalum-rhodium-species-assembled-through-a-novel-bifunctional-nhc-oh-ligand
#5
Ravi Srivastava, Raphaël Moneuse, Julien Petit, Paul-Alexis Pavard, Vincent Dardun, Madleen Rivat, Pauline Schiltz, Marius Solari, Erwann Jeanneau, Laurent Veyre, Chloé Thieuleux, Elsje Alessandra Quadrelli, Clement Camp
The straightforward synthesis of a new unsymmetrical hydroxyl-tethered N-heterocyclic carbene (NHC) ligand, HL is presented. The free ligand exhibits an unusual OH-carbene hydrogen-bonding interaction. This OH-carbene motif is used to yield i) the first tantalum complex displaying both a Fischer-type and a Schrock-type carbene ligand and ii) a unique NHC-based early-late heterobimetallic complex. More specifically, protonolysis chemistry between the ligand hydroxyl group and imido-alkyl or alkylidene-alkyl tantalum precursors yields rare monometallic tantalum-NHC complexes, Ta(XtBu)(L)(CH2tBu)2 (X = N, CH), where the alkoxy-carbene ligand acts as a chelate...
January 9, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29311622/dual-roles-of-trf1-in-tethering-telomeres-to-the-nuclear-envelope-and-protecting-them-from-fusion-during-meiosis
#6
Lina Wang, Zhaowei Tu, Chao Liu, Hongbin Liu, Philipp Kaldis, Zijiang Chen, Wei Li
Telomeres integrity is indispensable for chromosomal stability by preventing chromosome erosion and end-to-end fusions. During meiosis, telomeres attach to the inner nuclear envelope and cluster into a highly crowded microenvironment at the bouquet stage, which requires specific mechanisms to protect the telomeres from fusion. Here, we demonstrate that germ cell-specific knockout of a shelterin complex subunit, Trf1, results in arrest of spermatocytes at two different stages. The obliterated telomere-nuclear envelope attachment in Trf1-deficient spermatocytes impairs homologue synapsis and recombination, resulting in a pachytene-like arrest, while the meiotic division arrest might stem from chromosome end-to-end fusion due to the failure of recruiting meiosis specific telomere associated proteins...
January 8, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29311259/the-endosomal-trafficking-factors-corvet-and-escrt-suppress-plasma-membrane-residence-of-the-renal-outer-medullary-potassium-channel-romk
#7
Timothy D Mackie, Bo-Young Kim, Arohan R Subramanya, Daniel J Bain, Allyson F O'Donnell, Paul A Welling, Jeffrey L Brodsky
Protein trafficking can act as the primary regulatory mechanism for ion channels with high open probabilities, such as the renal outer medullary (ROMK) channel. ROMK, also known as Kir1.1 (KCNJ1), is the major route for potassium secretion into the pro-urine and plays an indispensable role in regulating serum potassium and urinary concentrations. However, the cellular machinery that regulates ROMK trafficking has not been fully defined. To identify regulators of the cell-surface population of ROMK, we expressed a pH-insensitive version of the channel in the budding yeast Saccharomyces cerevisiae...
January 8, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29297744/tgfb1-is-secreted-through-an-unconventional-pathway-dependent-on-the-autophagic-machinery-and-cytoskeletal-regulators
#8
Julian Nüchel, Sushmita Ghatak, Alexandra V Zuk, Anja Illerhaus, Matthias Mörgelin, Katrin Schönborn, Katrin Blumbach, Sara A Wickström, Thomas Krieg, Gerhard Sengle, Markus Plomann, Beate Eckes
TGFB1 (transforming growth factor beta 1) is a potent cytokine playing a driving role in development, fibrosis and cancer. It is synthesized as prodomain-growth factor complex that requires tethering to LTBP (latent transforming growth factor beta binding protein) for efficient secretion into the extracellular space. Upon release, this large latent complex is sequestered by anchorage to extracellular matrix (ECM) networks, from which the mature growth factor needs to be activated in order to reach its receptors and initiate signaling...
January 3, 2018: Autophagy
https://www.readbyqxmd.com/read/29284678/the-cue1-domain-of-the-snf2-like-chromatin-remodeler-smarcad1-mediates-its-association-with-krab-associated-protein-1-kap1-and-kap1-target-genes
#9
Dong Ding, Philipp Bergmaier, Parysatis Sachs, Marius Klangwart, Tamina Rückert, Nora Bartels, Jeroen Demmers, Mike Dekker, Raymond A Poot, Jacqueline E Mermoud
Chromatin in embryonic stem cells (ESCs) differs markedly from that in somatic cells, with ESCs exhibiting a more open chromatin configuration. Accordingly, ATP-dependent chromatin remodeling complexes are important regulators of ESC homeostasis. Depletion of the remodeler SMARCAD1, an ATPase of the SNF2 family, has been shown to affect stem cell state, but the mechanistic explanation for this effect is unknown. Here, we set out to gain further insights into the function of SMARCAD1 in mouse ESCs. We identified KRAB-associated protein 1 (KAP1) as the stoichiometric binding partner of SMARCAD1 in ESCs...
December 28, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29247758/ensemble-and-single-molecule-fret-studies-of-protein-synthesis
#10
Christine Wan-Jung Lai, Dmitri N Ermolenko
Protein synthesis is a complex, multi-step process that involves large conformational changes of the ribosome and protein factors of translation. Over the last decade, Förster resonance energy transfer (FRET) has become instrumental for studying structural rearrangements of the translational apparatus. Here, we discuss the design of ensemble and single-molecule (sm) FRET assays of translation. We describe a number of experimental strategies that can be used to introduce fluorophores into the ribosome, tRNA, mRNA and protein factors of translation...
December 13, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29237821/sequential-actions-of-phosphatidylinositol-phosphates-regulate-phagosome-lysosome-fusion
#11
Andreas Jeschke, Albert Haas
Phagosomes mature into phagolysosomes by sequential fusion with early endosomes, late endosomes, and lysosomes. Phagosome-with-lysosome fusion (PLF) results in the delivery of lysosomal hydrolases into phagosomes and in digestion of the cargo. The machinery that drives PLF has been little investigated. Using a cell-free system, we recently identified the phosphoinositide lipids (PIPs) phosphatidylinositol 3-phosphate [PI(3)P] and phosphatidylinositol 4-phosphate [PI(4)P] as regulators of PLF. We now report the identification and the PIP requirements of four distinct sub-reactions of PLF...
December 13, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29233296/protein-protein-and-protein-chromatin-interactions-of-ledgf-p75-as-novel-drug-targets
#12
REVIEW
Jolien Blokken, Jan De Rijck, Frauke Christ, Zeger Debyser
Lens epithelium-derived growth factor p75 (LEDGF/p75), a transcriptional co-activator, plays an important role in tethering protein complexes to the chromatin. Through this tethering function LEDGF/p75 is implicated in a diverse set of human diseases including HIV infection and mixed lineage leukemia, an aggressive form of cancer with poor prognosis. Here we provide an overview of recent progress in resolving protein-protein and protein-chromatin interaction mechanisms of LEDGF/p75. This review will focus on two well-characterized domains, the PWWP domain and the integrase binding domain (IBD)...
June 2017: Drug Discovery Today. Technologies
https://www.readbyqxmd.com/read/29231932/rhodium-mediated-asymmetric-transfer-hydrogenation-a-diastereo-and-enantioselective-synthesis-of-syn-%C3%AE-amido-%C3%AE-hydroxy-esters
#13
Long-Sheng Zheng, Charlène Férard, Phannarath Phansavath, Virginie Ratovelomanana-Vidal
The preparation of syn α-benzoylamido β-hydroxy esters through asymmetric transfer hydrogenation (ATH) with a tethered Rh(iii)-DPEN complex via dynamic kinetic resolution (DKR) has been developed for the first time starting from α-benzoylamido β-keto esters. A variety of α-benzoylamido β-keto esters were converted under mild conditions into the corresponding syn α-benzoylamino β-hydroxy esters with high yields (up to 98%) and diastereomeric ratios (up to >99 : 1 dr) as well as excellent enantioselectivities (up to >99% ee)...
December 12, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29229809/proteasomes-tether-to-two-distinct-sites-at-the-nuclear-pore-complex
#14
Sahradha Albert, Miroslava Schaffer, Florian Beck, Shyamal Mosalaganti, Shoh Asano, Henry F Thomas, Jürgen M Plitzko, Martin Beck, Wolfgang Baumeister, Benjamin D Engel
The partitioning of cellular components between the nucleus and cytoplasm is the defining feature of eukaryotic life. The nuclear pore complex (NPC) selectively gates the transport of macromolecules between these compartments, but it is unknown whether surveillance mechanisms exist to reinforce this function. By leveraging in situ cryo-electron tomography to image the native cellular environment of Chlamydomonas reinhardtii, we observed that nuclear 26S proteasomes crowd around NPCs. Through a combination of subtomogram averaging and nanometer-precision localization, we identified two classes of proteasomes tethered via their Rpn9 subunits to two specific NPC locations: binding sites on the NPC basket that reflect its eightfold symmetry and more abundant binding sites at the inner nuclear membrane that encircle the NPC...
December 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29220657/hnrnpk-recruits-pcgf3-5-prc1-to-the-xist-rna-b-repeat-to-establish-polycomb-mediated-chromosomal-silencing
#15
Greta Pintacuda, Guifeng Wei, Chloë Roustan, Burcu Anil Kirmizitas, Nicolae Solcan, Andrea Cerase, Alfredo Castello, Shabaz Mohammed, Benoît Moindrot, Tatyana B Nesterova, Neil Brockdorff
The Polycomb-repressive complexes PRC1 and PRC2 play a key role in chromosome silencing induced by the non-coding RNA Xist. Polycomb recruitment is initiated by the PCGF3/5-PRC1 complex, which catalyzes chromosome-wide H2A lysine 119 ubiquitylation, signaling recruitment of other PRC1 complexes, and PRC2. However, the molecular mechanism for PCGF3/5-PRC1 recruitment by Xist RNA is not understood. Here we define the Xist RNA Polycomb Interaction Domain (XR-PID), a 600 nt sequence encompassing the Xist B-repeat element...
December 7, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29218390/gemelli-obturator-complex-in-the-deep-gluteal-space-an-anatomic-and-dynamic-study
#16
Ramon Balius, Antonio Susín, Carles Morros, Montse Pujol, Dolores Pérez-Cuenca, Xavier Sala-Blanch
OBJECTIVE: To investigate the behavior of the sciatic nerve during hip rotation at subgluteal space. MATERIALS AND METHODS: Sonographic examination (high-resolution ultrasound machine at 5.0-14 MHZ) of the gemelli-obturator internus complex following two approaches: (1) a study on cadavers and (2) a study on healthy volunteers. The cadavers were examined in pronation, pelvis-fixed position by forcing internal and external rotations of the hip with the knee in 90° flexion...
December 7, 2017: Skeletal Radiology
https://www.readbyqxmd.com/read/29217648/the-interaction-of-enl-with-paf1-mitigates-polycomb-silencing-and-facilitates-murine-leukemogenesis
#17
Katrin Hetzner, Maria-Paz Garcia-Cuellar, Christian Büttner, Robert K Slany
ENL is a chromatin reader present in complexes stimulating transcriptional elongation. It is fused to MLL in leukemia and missense mutations have been identified in Wilms' tumor and AML. Here we demonstrate that ENL overcomes polycomb silencing through recruitment of PAF1 via the conserved YEATS domain that recognizes acetylated histone H3. PAF1 was responsible for anti-repressive activities of ENL in vitro and it determined the transforming potential of MLL-ENL. MLL-ENL target loci showed supraphysiological PAF1 binding, hyper-ubiquitination of histone H2B and hypomodification with H2AUb resulting in accelerated transcription rates...
December 7, 2017: Blood
https://www.readbyqxmd.com/read/29212755/model-based-image-analysis-of-a-tethered-brownian-fibre-for-shear-stress-sensing
#18
M T Gallagher, C V Neal, K P Arkill, D J Smith
The measurement of fluid dynamic shear stress acting on a biologically relevant surface is a challenging problem, particularly in the complex environment of, for example, the vasculature. While an experimental method for the direct detection of wall shear stress via the imaging of a synthetic biology nanorod has recently been developed, the data interpretation so far has been limited to phenomenological random walk modelling, small-angle approximation, and image analysis techniques which do not take into account the production of an image from a three-dimensional subject...
December 2017: Journal of the Royal Society, Interface
https://www.readbyqxmd.com/read/29208657/assembly-of-intermediates-for-rapid-membrane-fusion
#19
Max Harner, William Wickner
Membrane fusion is essential for intracellular protein sorting, cell growth, hormone secretion, and neurotransmission. Rapid membrane fusion requires tethering and Sec1-Munc18 (SM) function to catalyze R-, Qa-, Qb-, and Qc-SNARE complex assembly in trans, as well as SNARE engagement by the SNARE-binding chaperone Sec17/αSNAP. The hexameric vacuolar HOPS complex in the yeast Saccharomyces cerevisiae tethers membranes through its affinities for the membrane Rab GTPase Ypt7. HOPS also has specific affinities for the vacuolar SNAREs and catalyzes SNARE complex assembly, but the order of their assembly into a 4-SNARE complex is unclear...
December 5, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29204879/reconstitution-of-membrane-tethering-mediated-by-rab-family-small-gtpases
#20
REVIEW
Joji Mima
Membrane tethering is one of the most critical steps to determine the spatiotemporal specificity of membrane trafficking, which is the process to selectively transport proteins, lipids, and other biological molecules to the appropriate locations in eukaryotic cells, such as subcellular organelles, the plasma membrane, and the extracellular space. Based on genetic, cell biological, biochemical, and structural studies, Rab-family small GTPases and a number of Rab-interacting proteins (termed Rab effectors), including coiled-coil tethering proteins and multisubunit tethering complexes, have been proposed to be key protein components for membrane tethering...
December 4, 2017: Biophysical Reviews
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