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Tethering complex

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https://www.readbyqxmd.com/read/28338727/rin4-recruits-the-exocyst-subunit-exo70b1-to-the-plasma-membrane
#1
Peter Sabol, Ivan Kulich, Viktor Žárský
The exocyst is a conserved vesicle-tethering complex with principal roles in cell polarity and morphogenesis. Several studies point to its involvement in polarized secretion during microbial pathogen defense. In this context, we have found an interaction between the Arabidopsis EXO70B1 exocyst subunit, a protein which was previously associated with both the defense response and autophagy, and RPM1 INTERACTING PROTEIN 4 (RIN4), the best studied member of the NOI protein family and a known regulator of plant defense pathways...
February 20, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28337049/use-of-3d-printed-bone-plate-in-novel-technique-to-surgically-correct-hallux-valgus-deformities
#2
Kathryn E Smith, Kenneth M Dupont, David L Safranski, Jeremy Blair, Dawn Buratti, Vladimir Zeetser, Ryan Callahan, Jason Lin, Ken Gall
Three-dimensional (3-D) printing offers many potential advantages in designing and manufacturing plating systems for foot and ankle procedures that involve small, geometrically complex bony anatomy. Here, we describe the design and clinical use of a Ti-6Al-4V ELI bone plate (FastForward™ Bone Tether Plate, MedShape, Inc., Atlanta, GA) manufactured through 3-D printing processes. The plate protects the second metatarsal when tethering suture tape between the first and second metatarsals and is a part of a new procedure that corrects hallux valgus (bunion) deformities without relying on doing an osteotomy or fusion procedure...
September 2016: Techniques in Orthopaedics: TIO
https://www.readbyqxmd.com/read/28331962/sex-combs-reduced-scr-regulatory-region-of-drosophila-revisited
#3
Juan M Calvo-Martín, Montserrat Papaceit, Carmen Segarra
The Hox gene Sex combs reduced (Scr) is responsible for the differentiation of the labial and prothoracic segments in Drosophila. Scr is expressed in several specific tissues throughout embryonic development, following a complex path that must be coordinated by an equally complex regulatory region. Although some cis-regulatory modules (CRMs) have been identified in the Scr regulatory region (~75 kb), there has been no detailed and systematic study of the distinct regulatory elements present within this region...
March 22, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/28331092/monkeypox-virus-host-factor-screen-in-haploid-cells-identifies-essential-role-of-garp-complex-in-extracellular-virus-formation
#4
Susan Realegeno, Andreas S Puschnik, Amrita Kumar, Cynthia Goldsmith, Jillybeth Burgado, Suryaprakash Sambhara, Victoria A Olson, Darin Carroll, Inger Damon, Tetsuya Hirata, Taroh Kinoshita, Jan E Carette, Panayampalli Subbian Satheshkumar
Monkeypox virus (MPXV) is a human pathogen that is a member of the Orthopoxvirus genus, which includes Vaccinia virus and Variola virus (the causative agent of smallpox). Human monkeypox is considered an emerging zoonotic infectious disease. To identify host factors required for MPXV infection, we performed a genome-wide insertional mutagenesis screen in human haploid cells. The screen revealed several candidate genes, including those involved in Golgi trafficking, glycosaminoglycan biosynthesis and glycosylphosphatidylinositol (GPI) - anchor biosynthesis...
March 22, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28329369/long-term-results-up-to-14-years-of-the-clover-technique-for-the-treatment-of-complex-tricuspid-valve-regurgitation%C3%A2
#5
Michele De Bonis, Elisabetta Lapenna, Stefania Di Sanzo, Benedetto Del Forno, Federico Pappalardo, Alessandro Castiglioni, Luca Vicentini, Alberto Pozzoli, Ilaria Giambuzzi, Azeem Latib, Davide Schiavi, Giovanni La Canna, Ottavio Alfieri
OBJECTIVES: To report the long-term results of the clover technique for the treatment of complex forms of tricuspid regurgitation (TR). METHODS: Ninety-six consecutive patients (mean age 60 ± 16.4, left ventricular ejection fraction 58 ± 8.8%) with severe or moderately-severe TR due to important leaflets prolapse/flail (81 patients), tethering (13 patients) or mixed (2 patients) lesions underwent clover repair combined with annuloplasty. The aetiology of TR was degenerative in 74 cases (77...
February 23, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28325821/phosphorylation-of-the-exocyst-protein-exo84-by-tbk1-promotes-insulin-stimulated-glut4-trafficking
#6
Maeran Uhm, Merlijn Bazuine, Peng Zhao, Shian-Huey Chiang, Tingting Xiong, Sheelarani Karunanithi, Louise Chang, Alan R Saltiel
Insulin stimulates glucose uptake through the translocation of the glucose transporter GLUT4 to the plasma membrane. The exocyst complex tethers GLUT4-containing vesicles to the plasma membrane, a process that requires the binding of the G protein (heterotrimeric guanine nucleotide-binding protein) RalA to the exocyst complex. We report that upon activation of RalA, the protein kinase TBK1 phosphorylated the exocyst subunit Exo84. Knockdown of TBK1 blocked insulin-stimulated glucose uptake and GLUT4 translocation; knockout of TBK1 in adipocytes blocked insulin-stimulated glucose uptake; and ectopic overexpression of a kinase-inactive mutant of TBK1 reduced insulin-stimulated glucose uptake in 3T3-L1 adipocytes...
March 21, 2017: Science Signaling
https://www.readbyqxmd.com/read/28323853/afm-tirf-force-clamp-measurements-of-neurosecretory-vesicle-tethers-reveal-characteristic-unfolding-steps
#7
Mark C Harris, Dillon Cislo, Joan S Lenz, Christopher Umbach, Manfred Lindau
Although several proteins have been implicated in secretory vesicle tethering, the identity and mechanical properties of the components forming the physical vesicle-plasma membrane link remain unknown. Here we present the first experimental measurements of nanomechanical properties of secretory vesicle-plasma membrane tethers using combined AFM force clamp and TIRF microscopy on membrane sheets from PC12 cells expressing the vesicle marker ANF-eGFP. Application of pulling forces generated tether extensions composed of multiple steps with variable length...
2017: PloS One
https://www.readbyqxmd.com/read/28317958/the-collective-behavior-of-spring-like-motifs-tethered-to-a-dna-origami-nanostructure
#8
E-C Schöneweiß, B Saccà
Dynamic DNA nanotechnology relies on the integration of small switchable motifs at suitable positions of DNA nanostructures, thus enabling the manipulation of matter with nanometer spatial accuracy in a trigger-dependent fashion. Typical examples of such motifs are hairpins, whose elongation into duplexes can be used to perform long-range, translational movements. In this work, we used temperature-dependent FRET spectroscopy to determine the thermal stabilities of distinct sets of hairpins integrated into the central seam of a DNA origami structure...
March 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28314748/flight-control-of-fruit-flies-dynamic-response-to-optic-flow-and-headwind
#9
Kiaran K K Lawson, Mandyam V Srinivasan
Insects are magnificent fliers that are capable of performing many complex tasks such as speed regulation, smooth landings, and collision avoidance, even though their computational abilities are limited by their small brain. To investigate how flying insects respond to changes in wind speed and surrounding optic flow, the open-loop sensorimotor response of female Queensland fruit flies (Bactrocera tryoni) was examined. 136 flies were exposed to stimuli comprising sinusoidally varying optic flow and air flow (simulating forward movement) under tethered conditions in a virtual reality arena...
March 17, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/28314511/novel-platinum-iv-complexes-conjugated-with-a-wogonin-derivative-as-multi-targeted-anticancer-agents
#10
Xiaodong Qin, Gang Xu, Feihong Chen, Lei Fang, Shaohua Gou
Platinum-based complexes like cisplatin and oxaliplatin are well known the mainstay of chemotherapy regimens on clinic. Wogonin, a natural product that possesses wide biological activities, is now in phase I clinical test as an anticancer agent in China. Herein reported are a series of novel Pt(IV) complexes that conjugated a wogonin derivative (compound 3) to the axial position via a linker group. After being tethered to the platinum(IV) complexes, the wogonin derivative provided multiple anticancer effects, especially in compound 10, a fusion containing wogonin and cisplatin units...
March 7, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28306502/pacer-mediates-the-function-of-class-iii-pi3k-and-hops-complexes-in-autophagosome-maturation-by-engaging-stx17
#11
Xiawei Cheng, Xiuling Ma, Xianming Ding, Lin Li, Xiao Jiang, Zhirong Shen, She Chen, Wei Liu, Weihua Gong, Qiming Sun
Class III PI3-kinase (PI3KC3) is essential for autophagy initiation, but whether PI3KC3 participates in other steps of autophagy remains unknown. The HOPS complex mediates the fusion of intracellular vesicles to lysosome, but how HOPS specifically tethers autophagosome to lysosome remains elusive. Here, we report Pacer (protein associated with UVRAG as autophagy enhancer) as a regulator of autophagy. Pacer localizes to autophagic structures and positively regulates autophagosome maturation. Mechanistically, Pacer antagonizes Rubicon to stimulate Vps34 kinase activity...
March 16, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28304048/regioselective-c8-metalation-of-n-phosphine-tethered-adenine-derivatives-via-c8-h-activation
#12
D Brackemeyer, C Schulte To Brinke, F Roelfes, F E Hahn
Two new adenine derivatives bearing a N-methylene (1) or N-ethylene (2) tethered diphenylphosphine group have been reacted with [M(III)Cl2Cp*]2 (M = Ir, Rh) or [Ru(II)ClCp*]4. The formation of Werner-type phosphine complexes [3]-[6] and upon heating their subsequent rearrangement into complexes bearing an NH,NR-NHC^PRPh2 chelate ligand in [7]Cl (M = Ir) and [8] (M = Ru) have been observed.
March 17, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28300390/recruitment-and-regulation-of-the-non-ribosomal-peptide-synthetase-modifying-cytochrome-p450-involved-in-nikkomycin-biosynthesis
#13
Courtney E Wise, Thomas M Makris
The beta-hydroxylation of L-histidine is the first step in the biosynthesis of the imidazolone base of the antifungal drug nikkomycin. The cytochrome P450 (NikQ) hydroxylates the amino acid while it is appended via a phosphopantetheine linker to the non-ribosomal peptide synthetase (NRPS) NikP1. The latter enzyme is comprised of an MbtH, and single adenylation and thiolation domains, a minimal composition that allows for detailed binding and kinetics studies using an intact and homogeneous NRPS substrate. Electron paramagnetic resonance studies confirm that a stable complex is formed with NikQ with NikP1 when the amino acid is tethered...
March 16, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28297644/dna-endonuclease-complex-dynamics-by-simultaneous-fret-and-fluorophore-intensity-in-evanescent-field
#14
Marijonas Tutkus, Tomas Marciulionis, Giedrius Sasnauskas, Danielis Rutkauskas
The single-molecule Förster resonance energy transfer (FRET) is a powerful tool to study interactions and conformational changes of biological molecules in the distance range from a few to 10 nm. In this study, we demonstrate a method to augment this range with longer distances. The method is based on the intensity changes of a tethered fluorophore, diffusing in the exponentially decaying evanescent excitation field. In combination with FRET it allowed us to reveal and characterize the dynamics of what had been inaccessible conformations of the DNA-protein complex...
March 14, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28294274/discovery-of-hierarchical-superstructures-in-block-copolymers-by-integrating-different-liquid-crystalline-interactions
#15
Wei Wei, Zhichao Wu, Mingjun Huang, Chih-Hao Hsu, Yu Liu, Xinlin Zhang, Huiming Xiong
Hierarchically assembled superstructures of block copolymers are discovered by introduction of the competition of anisotropy attributed from liquid crystalline ordering and nano-phase separation. When the two prototypical fields of liquid crystals (LCs), smectics (S) and nematics (N), are adjoined within the framework of diblock copolymers (S-N) in a variety of precisely tuned compositions, a novel class of superlattices with an interdigitated array of smectic layers between the nearest lamellae or cylinders are observed...
March 15, 2017: Soft Matter
https://www.readbyqxmd.com/read/28292915/a-cascade-of-multiple-proteins-and-lipids-catalyzes-membrane-fusion
#16
William Wickner, Josep Rizo
Recent studies suggest revisions to the SNARE paradigm of membrane fusion. Membrane tethers and/or SNAREs recruit proteins of the Sec 1/Munc18 family to catalyze SNARE assembly into trans-complexes. SNARE-domain zippering draws the bilayers into immediate apposition and provides a platform to position fusion triggers such as Sec 17/α-SNAP and/or synaptotagmin, which insert their apolar "wedge" domains into the bilayers, initiating the lipid rearrangements of fusion.
March 15, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28285942/small-molecule-inhibitors-of-psd95-nnos-protein-protein-interactions-suppress-formalin-evoked-fos-protein-expression-and-nociceptive-behavior-in-rats
#17
Lawrence M Carey, Wan-Hung Lee, Tannia Gutierrez, Pushkar M Kulkarni, Ganesh A Thakur, Yvonne Y Lai, Andrea G Hohmann
Excessive activation of NMDA receptor (NMDAR) signaling within the spinal dorsal horn contributes to central sensitization and the induction and maintenance of pathological pain states. However, direct antagonism of NMDARs produces undesirable side effects which limit their clinical use. NMDAR activation produces central sensitization, in part, by initiating a signaling cascade that activates the enzyme neuronal nitric oxide synthase (nNOS) and generates the signaling molecule nitric oxide (NO). NMDAR-mediated activation of nNOS requires a scaffolding protein, postsynaptic density protein 95 kDa (PSD95), which tethers nNOS to NMDARs...
March 7, 2017: Neuroscience
https://www.readbyqxmd.com/read/28284242/epstein-barr-virus-nuclear-antigen-1-interacts-with-regulator-of-chromosome-condensation-1-dynamically-throughout-the-cell-cycle
#18
Thibaut Deschamps, Quentin Bazot, Derek M Leske, Ruth MacLeod, Dimitri Mompelat, Lionel Tafforeau, Vincent Lotteau, Vincent Maréchal, George S Baillie, Henri Gruffat, Joanna B Wilson, Evelyne Manet
The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is a sequence-specific DNA-binding protein that plays an essential role in viral episome replication and segregation, by recruiting the cellular complex of DNA replication onto the origin (oriP) and by tethering the viral DNA onto the mitotic chromosomes. Whereas the mechanisms of viral DNA replication are well documented, those involved in tethering EBNA1 to the cellular chromatin are far from being understood. Here, we have identified regulator of chromosome condensation 1 (RCC1) as a novel cellular partner for EBNA1...
February 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28264817/regulation-of-cell-polarity-by-exocyst-mediated-trafficking
#19
Noemi Polgar, Ben Fogelgren
One requirement for establishing polarity within a cell is the asymmetric trafficking of intracellular vesicles to the plasma membrane. This tightly regulated process creates spatial and temporal differences in both plasma membrane composition and the membrane-associated proteome. Asymmetric membrane trafficking is also a critical mechanism to regulate cell differentiation, signaling, and physiology. Many eukaryotic cell types use the eight-protein exocyst complex to orchestrate polarized vesicle trafficking to certain membrane locales...
March 6, 2017: Cold Spring Harbor Perspectives in Biology
https://www.readbyqxmd.com/read/28257190/oxoiron-iv-tetramethylcyclam-complexes-with-axial-carboxylate-ligands-effect-of-tethering-the-carboxylate-on-reactivity
#20
Jennifer O Bigelow, Jason England, Johannes E M N Klein, Erik R Farquhar, Jonathan R Frisch, Marlène Martinho, Debasish Mandal, Eckard Münck, Sason Shaik, Lawrence Que
Oxoiron(IV) species are implicated as reactive intermediates in nonheme monoiron oxygenases, often acting as the agent for hydrogen-atom transfer from substrate. A histidine is the most likely ligand trans to the oxo unit in most enzymes characterized thus far but is replaced by a carboxylate in the case of isopenicillin N synthase. As the effect of a trans carboxylate ligand on the properties of the oxoiron(IV) unit has not been systematically studied, we have synthesized and characterized four oxoiron(IV) complexes supported by the tetramethylcyclam (TMC) macrocycle and having a carboxylate ligand trans to the oxo unit...
March 3, 2017: Inorganic Chemistry
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