Sabah N A Hussain, Mahroo Mofarrahi, Ioanna Sigala, Ho Cheol Kim, Theodoros Vassilakopoulos, Francois Maltais, Ion Bellenis, Rakesh Chaturvedi, Stewart B Gottfried, Peter Metrakos, Gawiyou Danialou, Stefan Matecki, Samir Jaber, Basil J Petrof, Peter Goldberg
RATIONALE: Controlled mechanical ventilation (CMV) results in atrophy of the human diaphragm. The autophagy-lysosome pathway (ALP) contributes to skeletal muscle proteolysis, but its contribution to diaphragmatic protein degradation in mechanically ventilated patients is unknown. OBJECTIVES: To evaluate the autophagy pathway responses to CMV in the diaphragm and limb muscles of humans and to identify the roles of FOXO transcription factors in these responses. METHODS: Muscle biopsies were obtained from nine control subjects and nine brain-dead organ donors...
December 1, 2010: American Journal of Respiratory and Critical Care Medicine