keyword
MENU ▼
Read by QxMD icon Read
search

Ion mobility spectrometry

keyword
https://www.readbyqxmd.com/read/28933014/size-exclusion-chromatography-ion-mobility-mass-spectrometry-coupling-a-step-toward-structural-biology
#1
Guillaume Van der Rest, Frédéric Halgand
Noncovalent interactions are essential for the structural organization of biomacromolecules in cells. For this reason, the study of the biophysical, dynamic, and architectural interactions among biomacromolecules is essential. Since mass spectrometry requires compatible solutions while preserving the noncovalent bonding network, we envisioned that size exclusion chromatography coupled with ion mobility and mass spectrometry would be a valuable technique to desalt the initial sample and provide solution and gas-phase structural information in a single stage experiment...
September 20, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28932891/identification-of-terpenes-and-essential-oils-by-means-of-static-headspace-gas-chromatography-ion-mobility-spectrometry
#2
Roman Rodríguez-Maecker, Eduardo Vyhmeister, Stefan Meisen, Antonio Rosales Martinez, Andriy Kuklya, Ursula Telgheder
Static headspace gas chromatography-ion mobility spectrometry (SHS GC-IMS) is a relatively new analytical technique that has considerable potential for analysis of volatile organic compounds (VOCs). In this study, SHS GC-IMS was used for the identification of the major terpene components of various essential oils (EOs). Based on the data obtained from 25 terpene standards and 50 EOs, a database for fingerprint identification of characteristic terpenes and EOs was generated utilizing SHS GC-IMS for authenticity testing of fragrances in foods, cosmetics, and personal care products...
September 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28930473/molecular-insights-into-human-serum-albumin-as-a-receptor-of-amyloid-%C3%AE-in-the-extracellular-region
#3
Tae Su Choi, Hyuck Jin Lee, Jong Yoon Han, Mi Hee Lim, Hugh I Kim
Regulation of amyloid-β (Aβ) aggregation by metal ions and proteins is essential for understanding the pathology of Alzheimer's disease (AD). Human serum albumin (HSA), a regulator of metal and protein transportation, can modulate metal-Aβ interactions and Aβ aggregation in human fluid; however, the molecular mechanisms for such activities remain unclear. Herein, we report the molecular-level complexation between Zn(II), Cu(II), Aβ, and HSA, which is able to alter the aggregation and cytotoxicity of Aβ peptides and induce their cellular transportation...
September 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28929691/-deuterated-surrogate-and-uplc-ms-ms-method-for-simultaneous-determination-of-22-endocannabinoids-in-ovariectomized-rat-plasma
#4
Shi-Xie Xiang, Ying Xu, Jing-Jing Zhu, Zhi-Min Wang, Dong Zhang, Liang-Mian Chen, Wei-Hong Feng
A new method based on ultraperformance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for the simultaneous determination of 22 endocannabinoids(eCBs) and relevant compounds in ovariectomized rat plasma. After being extracted by solid-phase column(SPE), the plasma samples were detected by using UPLC-MS/MS. Analysis was carried out with ACQUITY UPLC BEH C₁₈ column. The mobile phase was 0.1% acetic acid solution(A)-acetonitrile and isopropanol(9∶1, B) for the gradient elution. In the positive ion multiple reaction monitoring(MRM) mode, deuterated reagents were taken as standard alternatives to calculate recoveries and simultaneously quantify 22 endocannabinoids...
November 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28922569/strategies-for-analysis-of-isomeric-peptides
#5
Erik T Jansson
This review presents an overview and recent progress of strategies for detecting isomerism in peptides, with focus on d/l epimerization and the various isomers that the presence of an aspartic acid residue may yield in a protein or peptide. While mass spectrometry has become a majorly used method of choice within proteomics, isomerism is inherently difficult to analyze because it is a modification that does not yield any change in mass of the analyte. Here, several techniques used for analysis of peptide isomerism are discussed, including enzymatic assays, liquid chromatography and capillary electrophoresis...
September 18, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28918324/simultaneous-determination-and-pharmacokinetic-study-of-twelve-bioactive-compounds-in-rat-plasma-after-intravenous-administration-of-xuebijing-injection-by-uhplc-q-orbitrap-hrms
#6
Lihua Zuo, Qiuyue Zhong, Zhenhui Wang, Zhi Sun, Lin Zhou, Zhuolun Li, Tanye Xu, Yingying Shi, Jinfa Tang, Shuzhang Du, Xiaojian Zhang
Xuebijing injection (XBJ) is a traditional Chinese herbal prescription widely used in the treatment of sepsis. Extensive studies revealed that the major bioactive constituents of XBJ injection, including phenolic acids, flavonoids, monoterpene glycosides, lactones and organic acids, play an important role in the treatment. In this study, a rapid, sensitive and accurate ultra high performance liquid chromatography - Q Exactive hybrid quadrupole - orbitrap high-resolution accurate mass spectrometry (UHPLC-Q-Orbitrap HRMS) method was developed for simultaneous determination of twelve bioactive compounds in rat plasma after intravenous administration of XBJ injection...
September 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28918323/quantitative-determination-of-carfilzomib-in-mouse-plasma-by-liquid-chromatography-tandem-mass-spectrometry-and-its-application-to-a-pharmacokinetic-study
#7
Jee Sun Min, Jiseon Kim, Jung Ho Kim, Doyun Kim, Yu Fen Zheng, Ji Eun Park, Wooin Lee, Soo Kyung Bae
A highly sensitive and rapid LC-MS/MS method was developed and validated to determine the levels of carfilzomib in mice plasma by using chlorpropamide as an internal standard. Carfilzomib and chlorpropamide were extracted from 5 μL of plasma after protein precipitation with acetonitrile. Chromatographic separation was performed on Phenomenex Luna C18 column (50×2.0mm id, 3μm). The mobile phase consisted of 0.1% formic acid in acetonitrile -0.1% formic acid in water (1:1v/v) and the flow rate was 0.3mL/min...
September 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28917748/selectivity-improvement-of-positive-photoionization-ion-mobility-spectrometry-for-rapid-detection-of-organophosphorus-pesticides-by-switching-dopant-concentration
#8
Qinghua Zhou, Jia Li, Bin Wang, Shuang Wang, Haiyang Li, Jinyuan Chen
Ion mobility spectrometry (IMS) opened a potential avenue for the rapid detection of organophosphorus pesticides (OPPs), though an improved selectivity of stand-alone IMS was still in high demand. In this study, a stand-alone positive photoionization ion mobility spectrometry (PP-IMS) apparatus was constructed for the rapid detection of OPPs with acetone as dopant. The photoionization of acetone molecules was induced by the ultraviolet irradiation to produce the reactant ions (Ac)2H(+), which were employed to ionize the OPPs including fenthion, imidan, phosphamidon, dursban, dimethoate and isocarbophos via the proton transfer reaction...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917733/stabilization-of-gas-phase-uranyl-complexes-enables-rapid-speciation-using-electrospray-ionization-and-ion-mobility-mass-spectrometry
#9
Austen L Davis, Brian H Clowers
Significant challenges exist when characterizing f-element complexes in solution using traditional approaches such as electrochemical and spectroscopic techniques as they do not always capture information for lower abundance species. However, provided a metal-complex with sufficient stability, soft ionization techniques such as electrospray offer a means to quantify and probe the characteristics of such systems using mass spectrometry. Unfortunately, the gas-phase species observed in ESI-MS systems do not always reflect the solution phase distributions due to the inherent electrochemical mechanism of the electrospray process, ion transfer from ambient to low pressures conditions, and other factors that are related to droplet evaporation...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28911626/high-throughput-liquid-chromatography-tandem-mass-spectrometry-method-for-simultaneous-determination-of-fampridine-paroxetine-and-quinidine-in-rat-plasma-application-to-in%C3%A2-vivo-perfusion-study
#10
Suneetha Achanti, Raja Rajeswari Katta
A selective and high-throughput liquid chromatography-mass spectrometry method has been developed and validated for the simultaneous quantification of paroxetine, fampridine, and quinidine in rat plasma using imipramine as an internal standard. Following protein precipitation extraction, the analytes and internal standard were run on XBridge C18 column (150 mm × 4.6 mm, 5 μm) using a gradient mobile phase consisting of 5mM ammonium formate in water (pH 9.0) and acetonitrile in a flow gradience program...
October 2016: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28910313/a-detailed-comparison-of-analysis-processes-for-mcc-ims-data-in-disease-classification-automated-methods-can-replace-manual-peak-annotations
#11
Salome Horsch, Dominik Kopczynski, Elias Kuthe, Jörg Ingo Baumbach, Sven Rahmann, Jörg Rahnenführer
MOTIVATION: Disease classification from molecular measurements typically requires an analysis pipeline from raw noisy measurements to final classification results. Multi capillary column-ion mobility spectrometry (MCC-IMS) is a promising technology for the detection of volatile organic compounds in the air of exhaled breath. From raw measurements, the peak regions representing the compounds have to be identified, quantified, and clustered across different experiments. Currently, several steps of this analysis process require manual intervention of human experts...
2017: PloS One
https://www.readbyqxmd.com/read/28910102/effects-of-charge-state-charge-distribution-and-structure-on-the-ion-mobility-of-protein-ions-in-helium-gas-results-from-trajectory-method-calculations
#12
Kenneth J Laszlo, Matthew F Bush
Collision cross section (Ω) values of gas-phase ions of proteins and protein complexes are used to probe the structures of the corresponding species in solution. Ions of many proteins exhibit increasing Ω values with increasing charge state, but most Ω values calculated for protein ions have used simple collision models that do not explicitly account for charge. Here we use a combination of ion mobility mass spectrometry experiments using helium gas and trajectory method calculations to characterize the extents to which increases in experimental Ω values with increasing charge state may be attributed to increased momentum transfer concomitant with enhanced long-range interactions between the protein ion and helium atoms...
September 14, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28906001/overcoming-interference-of-plasma-phospholipids-using-hybridspe-for-the-determination-of-trimetazidine-by-uplc-ms-ms
#13
Pravin G Vanol, Manish Yadav, Mallika Sanyal, Priyanka A Shah, Pranav S Shrivastav
An improved, precise and reliable ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the quantification of trimetazidine, using trimetazidine-d8 as the internal standard (IS). Interference due to plasma phospholipids during sample preparation was overcome by using hybrid solid phase extraction-phospholipid ultra cartridge. The mean extraction recovery of trimetazidine (98.66 %) and trimetazidine-d8 (97.63 %) from spiked plasma was consistent and reproducible...
September 14, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28901031/isomer-separation-and-effect-of-the-polymerization-degree-on-the-gas-phase-structure-of-chondroitin-sulfate-oligosaccharides-analyzed-by-ion-mobility-tandem-mass-spectrometry
#14
Salomé Poyer, Chrystel Lopin-Bon, Jean-Claude Jacquinet, Jean-Yves Salpin, Régis Daniel
RATIONALE: Chondroitin sulfate (CS) glycosaminoglycans are bioactive sulfated polysaccharides comprising repeating units of uronic acid and N-acetyl galactose sulfated at various positions. The optimal length and sulfation pattern of the CS bioactive sequences remain elusive so that structure-activity relationship cannot be easily established. Development of efficient analytical methods allowing the differentiation of the various sulfation patterns of CS sequences is therefore of particular importance to correlate their biological functions to the sulfation pattern...
September 12, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28900836/using-in-silico-fragmentation-to-improve-routine-residue-screening-in-complex-matrices
#15
Anton Kaufmann, Patrick Butcher, Kathryn Maden, Stephan Walker, Mirjam Widmer
Targeted residue screening requires the use of reference substances in order to identify potential residues. This becomes a difficult issue when using multi-residue methods capable of analyzing several hundreds of analytes. Therefore, the capability of in silico fragmentation based on a structure database ("suspect screening") instead of physical reference substances for routine targeted residue screening was investigated. The detection of fragment ions that can be predicted or explained by in silico software was utilized to reduce the number of false positives...
September 12, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28900708/apparent-activation-energies-of-protein-protein-complex-dissociation-in-the-gas-phase-determined-by-electrospray-mass-spectrometry
#16
Yelena Yefremova, F Teresa I Melder, Bright D Danquah, Kwabena F M Opuni, Cornelia Koy, Alexandra Ehrens, David Frommholz, Harald Illges, Knut Koelbel, Frank Sobott, Michael O Glocker
We have developed a method to determine apparent activation energies of dissociation for ionized protein-protein complexes in the gas phase using electrospray ionization mass spectrometry following the Rice-Ramsperger-Kassel-Marcus quasi-equilibrium theory. Protein-protein complexes were formed in solution, transferred into the gas phase, and separated from excess free protein by ion mobility filtering. Afterwards, complex disassembly was initiated by collision-induced dissociation with step-wise increasing energies...
September 12, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28889248/towards-discovery-and-targeted-peptide-biomarker-detection-using-nanoesi-tims-tof-ms
#17
Alyssa Garabedian, Paolo Benigni, Cesar E Ramirez, Erin S Baker, Tao Liu, Richard D Smith, Francisco Fernandez-Lima
In the present work, the potential of trapped ion mobility spectrometry coupled to TOF mass spectrometry (TIMS-TOF MS) for discovery and targeted monitoring of peptide biomarkers from human-in-mouse xenograft tumor tissue was evaluated. In particular, a TIMS-MS workflow was developed for the detection and quantification of peptide biomarkers using internal heavy analogs, taking advantage of the high mobility resolution (R = 150-250) prior to mass analysis. Five peptide biomarkers were separated, identified, and quantified using offline nanoESI-TIMS-CID-TOF MS; the results were in good agreement with measurements using a traditional LC-ESI-MS/MS proteomics workflow...
September 9, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28888687/temporary-removal-rapid-screening-of-abused-drugs-by-direct-analysis-in-real-time-dart-coupled-to-time-of-flight-mass-spectrometry-tof-ms-combined-with-ion-mobility-spectrometry-ims
#18
Ru Lian, Zhongping Wu, Xiaobao Lv, Yulan Rao, Haiyang Li, Jinghua Li, Rong Wang, Chunfang Ni, Yurong Zhang
The publisher regrets that this article has been temporarily removed. A replacement will appear as soon as possible in which the reason for the removal of the article will be specified, or the article will be reinstated. The full Elsevier Policy on Article Withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal.
July 13, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28886522/quantification-of-the-antimalarial-drug-pyronaridine-in-whole-blood-using-lc-ms-ms-increased-sensitivity-resulting-from-reduced-non-specific-binding
#19
Daniel Blessborn, Karnrawee Kaewkhao, Lijiang Song, Nicholas J White, Nicholas P J Day, Joel Tarning
Malaria is one of the most important parasitic diseases of man. The development of drug resistance in malaria parasites is an inevitable consequence of their widespread and often unregulated use. There is an urgent need for new and effective drugs. Pyronaridine is a known antimalarial drug that has received renewed interest as a partner drug in artemisinin-based combination therapy. To study its pharmacokinetic properties, particularly in field settings, it is necessary to develop and validate a robust, highly sensitive and accurate bioanalytical method for drug measurements in biological samples...
August 26, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28881131/where-ion-mobility-and-molecular-dynamics-meet-to-unravel-the-un-folding-mechanisms-of-an-oligorotaxane-molecular-switch
#20
Emeline Hanozin, Benoit Mignolet, Denis Morsa, Damien Sluysmans, Anne-Sophie Duwez, J Fraser Stoddart, Françoise Remacle, Edwin De Pauw
At the interface between foldamers and mechanically interlocked molecules, oligorotaxanes exhibit a spring-like folded secondary structure with remarkable mechanical and physicochemical properties. Among these properties, the ability of oligorotaxanes to act as molecular switches through controlled modulations of their spatial extension over (un)folding dynamics is of particular interest. The present study aims to assess and further characterize this remarkable feature in the gas phase using mass spectrometry tools...
September 13, 2017: ACS Nano
keyword
keyword
45838
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"