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Ion mobility spectrometry

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https://www.readbyqxmd.com/read/27908495/analysis-of-low-abundant-trehalose-6-phosphate-and-related-metabolites-in-medicago-truncatula-by-hydrophilic-interaction-liquid-chromatography-triple-quadrupole-mass-spectrometry
#1
Ana Teresa Mata, Tiago Filipe Jorge, João Ferreira, Maria do Rosário Bronze, Diana Branco, Pedro Fevereiro, Susana Araújo, Carla António
Trehalose-6-phosphate (T6P) is an important signaling metabolite involved in plant growth control that inhibits the sucrose nonfermenting-1-related protein kinase 1 (SnRK1), a key regulator of energy and carbon metabolism in plants. The quantification of T6P in plant tissues is fundamental to improve our understanding of sugar signaling and the links between plant growth and development in response to stress conditions. However, the almost undetectable levels of T6P together with the complex plant matrix and the presence of T6P isomers such as sucrose-6-phosphate (S6P), makes the detection of this metabolite challenging...
November 21, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27902942/a-simple-and-sensitive-liquid-chromatography-tandem-mass-spectrometry-method-for-trans-%C3%AE%C2%B5-viniferin-quantification-in-mouse-plasma-and-its-application-to-a-pharmacokinetic-study-in-mice
#2
Jiseon Kim, Jee Sun Min, Doyun Kim, Yu Fen Zheng, Karabasappa Mailar, Won Jun Choi, Choongho Lee, Soo Kyung Bae
In this study, a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of trans-ε-viniferin in small volumes (10μl) of mouse plasma using chlorpropamide as an internal standard was developed and validated. Plasma samples were precipitated with acetonitrile and separated using an Eclipse Plus C18 column (100×4.6mm, 1.8-μm) with a mobile phase consisting of 0.1% formic acid in acetonitrile and 0.1% formic acid in water (60:40v/v) at a flow rate of 0.5ml/min...
November 24, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27900858/rapid-identification-of-triphenylmethane-dyes-by-ion-mobility-time-of-flight-mass-spectrometry
#3
Alexey A Sysoev, Sergey S Poteshin, Denis M Chernyshev, Alexander A Sysoev
An ion mobility time-of-flight mass spectrometry (IM-TOFMS)-based method has been preliminarily investigated for the identification of triphenylmethane ballpoint pen dyes on paper. The dyes were sampled from one-year-old ballpoint pen ink entries. The entries were written on paper documents stored in the dark in a bookcase. Sample solutions were prepared by extraction of dyes in a vial. Basic violet 2, Methyl violet 6B, Methyl violet 2B and Crystal violet were characterized by IM-TOFMS. Since the ballpoint ink dyes contain ionic compounds, the studied compounds were expected to form stable peaks in the atmospheric pressure drift tube ion mobility spectrometry, and this was experimentally verified...
2016: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/27896757/lc-ms-e-multiplex-ms-ms-ion-mobility-and-label-free-quantitation-in-clinical-proteomics
#4
Gustavo Henrique Martins Ferreira Souza, Paul C Guest, Daniel Martins-de-Souza
Proteomic tools can only be implemented in clinical settings if high-throughput, automated, sensitive, and accurate methods are developed. This has driven researchers to the edge of mass spectrometry (MS)-based proteomics capacity. Here we provide an overview of recent achievements in mass spectrometric technologies and instruments. This includes development of high and ultra definition-MS(E) (HDMS(E) and UDMS(E)) through implementation of ion mobility (IM) MS towards sensitive and accurate label-free proteomics using ultra performance liquid chromatography (UPLC)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27884874/development-and-validation-of-a-sensitive-lc-ms-ms-method-for-quantification-of-mogrol-in-rat-plasma-and-application-to-pharmacokinetic-study
#5
Zuliang Luo, Kailun Zhang, Hongwu Shi, Yuhua Guo, Xiaojun Ma, Feng Qiu
Mogrol, the aglycone of mogrosides, is a potential pharmacologically active ingredient isolated from the fruits of Siraitia grosvenorii The aim of this study was to develop and validate an LC-MS-MS method for the quantification of mogrol in rat plasma. Protein precipitation extraction procedure using methanol/water (1:1, v/v) was employed to extract mogrol from rat plasma. Chromatographic separation was performed on a reverse-phase Agilent Zorbax XDB C18 column (50 mm × 2.1 mm, 3.5 μm) with gradient elution using a mobile phase containing methanol and water, both of which contained 0...
November 24, 2016: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/27872613/rhamnolipids-produced-by-indigenous-acinetobacter-junii-from-petroleum-reservoir-and-its-potential-in-enhanced-oil-recovery
#6
Hao Dong, Wenjie Xia, Honghong Dong, Yuehui She, Panfeng Zhu, Kang Liang, Zhongzhi Zhang, Chuanfu Liang, Zhaozheng Song, Shanshan Sun, Guangqing Zhang
Biosurfactant producers are crucial for incremental oil production in microbial enhanced oil recovery (MEOR) processes. The isolation of biosurfactant-producing bacteria from oil reservoirs is important because they are considered suitable for the extreme conditions of the reservoir. In this work, a novel biosurfactant-producing strain Acinetobacter junii BD was isolated from a reservoir to reduce surface tension and emulsify crude oil. The biosurfactants produced by the strain were purified and then identified via electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR-MS)...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27871801/lipid-profiling-of-polarized-human-monocyte-derived-macrophages
#7
J Rafael Montenegro-Burke, Jessica A Sutton, Lisa M Rogers, Ginger L Milne, John A McLean, David M Aronoff
The highly orchestrated transcriptional and metabolic reprogramming during activation drastically transforms the main functions and physiology of human macrophages across the polarization spectrum. Lipids, for example, can modify protein function by acting remotely as signaling molecules but also locally by altering the physical properties of cellular membranes. These changes play key roles in the functions of highly plastic immune cells due to their involvement in inflammation, immune responses, phagocytosis and wound healing processes...
November 15, 2016: Prostaglandins & Other Lipid Mediators
https://www.readbyqxmd.com/read/27869199/ion-mobility-spectrometry-as-a-simple-and-rapid-method-to-measure-the-plasma-propofol-concentrations-for-intravenous-anaesthesia-monitoring
#8
Xin Wang, Qinghua Zhou, Dandan Jiang, Yulei Gong, Enyou Li, Haiyang Li
The plasma propofol concentration is important information for anaesthetists to monitor and adjust the anaesthesia depth for patients during a surgery operation. In this paper, a stand-alone ion mobility spectrometer (IMS) was constructed for the rapid measurement of the plasma propofol concentrations. Without any sample pre-treatment, the plasma samples were dropped on a piece of glass microfiber paper and then introduced into the IMS cell by the thermal desorption directly. Each individual measurement could be accomplished within 1 min...
November 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27865831/rutin-suppresses-human-amylin-hiapp-misfolding-and-oligomer-formation-in-vitro-and-ameliorates-diabetes-and-its-impacts-in-human-amylin-hiapp-transgenic-mice
#9
Jacqueline F Aitken, Kerry M Loomes, Isabel Riba-Garcia, Richard D Unwin, Gordana Prijic, Ashley S Phillips, Anthony R J Phillips, Donghai Wu, Sally D Poppitt, Ke Ding, Perdita E Barran, Andrew W Dowsey, Garth J S Cooper
Pancreatic islet β-cells secrete the hormones insulin and amylin, and defective β-cell function plays a central role in the pathogenesis of type-2 diabetes (T2D). Human amylin (hA, also termed hIAPP) misfolds and forms amyloid aggregates whereas orthologous mouse amylin does neither. Furthermore, hA elicits apoptosis in cultured β-cells and β-cell death in ex-vivo islets. In addition, hA-transgenic mice that selectively express hA in their β-cells, manifest β-cell apoptosis and progressive islet damage that leads to diabetes closely resembling that in patients with T2D...
November 16, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27862997/identification-of-l-carnitine-and-its-impurities-in-food-supplement-formulations-by-online-column-switching-liquid-chromatography-coupled-with-linear-ion-trap-mass-spectrometry
#10
Hang Wang, Sijun Xie
The identification of impurities in l-carnitine by mass spectrometry is difficult because derivative reagents or ion pair reagents are usually used to separate and increase the retention of l-carnitine on the reversed-phase column. In this study, four impurities including 3-chloro-2-hydroxy-N,N,N-trimethylpropan-1-aminium, 3-cyano-2-hydroxy-N,N,N-trimethylpropan-1-aminium, 3-carboxy-N,N,N-trimethylprop-2-en-1-aminium and 4-chloro-2,3,4-trihydroxy-N,N,N-trimethylbutan-1-aminium were identified in l-carnitine and its tablets by using two-dimensional column-switching high-performance liquid chromatography coupled with linear ion trap mass spectrometry...
November 15, 2016: Journal of Separation Science
https://www.readbyqxmd.com/read/27862777/deriving-structural-information-from-experimentally-measured-data-on-biomolecules
#11
REVIEW
Wilfred F van Gunsteren, Jane R Allison, Xavier Daura, Jožica Dolenc, Niels Hansen, Alan E Mark, Chris Oostenbrink, Victor H Rusu, Lorna J Smith
During the past half century, the number and accuracy of experimental techniques that can deliver values of observables for biomolecular systems have been steadily increasing. The conversion of a measured value Q(exp) of an observable quantity Q into structural information is, however, a task beset with theoretical and practical problems: 1) insufficient or inaccurate values of Q(exp) , 2) inaccuracies in the function Q(r→) used to relate the quantity Q to structure r→ , 3) how to account for the averaging inherent in the measurement of Q(exp) , 4) how to handle the possible multiple-valuedness of the inverse r→(Q) of the function Q(r→) , to mention a few...
November 8, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27862466/role-of-ammonium-in-the-ionization-of-phosphatidylcholines-during-electrospray-mass-spectrometry
#12
Michael D Rush, Richard B van Breemen
RATIONALE: Electrospray mass spectrometry methods for the analysis of phosphatidylcholines (PCs) routinely include ammonium acetate or ammonium formate in the mobile phase. In an effort to justify and optimize the use of these additives, we investigated possible functions of ammonium compounds in the ionization of PCs. METHODS: Because PCs contain a quaternary amine, the role of ammonium in neutralizing the negatively charged phosphate group was investigated by using deuterated ammonium acetate, adjusting the pH, varying the organic solvent composition, and by comparing the additives ammonium acetate, ammonium formate and ammonium bicarbonate...
November 11, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/27859537/development-of-a-targeted-method-for-quantification-of-gypenoside-xlix-in-rat-plasma-using-spe-and-lc-ms-ms
#13
Song Guo, Chengxu Sui, Ying Ma
A sensitive, selective and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of gypenoside XLIX, a naturally occurring gypenoside of Gynostemma pentaphyllum in rat plasma and then validated according to FDA Guidance for industry-bioanalytical method validation. Plasma samples were prepared by a simple solid-phase extraction. Separation was performed on a Waters XBridgeTM BEH C18 chromatography column (4.6 mm × 50 mm, 2.5 µm) using a mobile phase of acetonitrile and water (62...
November 16, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27856639/the-atypical-dual-specificity-phosphatase-hyvh1-associates-with-multiple-ribonucleoprotein-particles
#14
Qiudi Geng, Besa Xhabija, Colleen Knuckle, Christopher A Bonham, Panayiotis O Vacratsis
Human YVH1 (hYVH1), also known as dual specificity phosphatase 12 (DUSP12) is a poorly characterized atypical dual specificity phosphatase widely conserved throughout evolution. Recent findings have demonstrated that hYVH1 expression affects cellular DNA content and is a novel cell survival phosphatase preventing both thermal and oxidative stress induced cell death, while studies in yeast have established YVH1 as a novel 60S ribosome biogenesis factor. In this study, we have isolated novel hYVH1 associating proteins from human U2OS osteosarcoma cells using affinity chromatography coupled to mass spectrometry employing ion mobility separation...
November 17, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27855912/target-identification-of-volatile-metabolites-to-allow-the-differentiation-of-lactic-acid-bacteria-by-gas-chromatography-ion-mobility-spectrometry
#15
Janneth Gallegos, Cristina Arce, Rafael Jordano, Lourdes Arce, Luis M Medina
The purpose of this work was to study the potential of gas chromatography-ion mobility spectrometry (GC-IMS) to differentiate lactic acid bacteria (LAB) through target identification and fingerprints of volatile metabolites. The LAB selected were used as reference strains for their influence in the flavour of cheese. The four strains of LAB can be distinguished by the fingerprints generated by the volatile organic compounds (VOCs) emitted. 2-butanone, 2-pentanone, 2-heptanone and 3-methyl-1-butanol were identified as relevant VOCs for Lactobacillus casei and Lactobacillus paracasei subsp...
April 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27853790/supramolecular-influence-on-cis-trans-isomerization-probed-by-ion-mobility-spectrometry
#16
Izabella Czerwinska, Alexander Kulesza, Changmin Choi, Fabien Chirot, Anne-Laure Simon, Johann Far, Chistopher Kune, Edwin de Pauw, Philippe Dugourd
We used tandem ion mobility spectrometry measurements to investigate how the photo-isomerization of a chromophore (a methylpyridinium derivative) is affected by the complexation with a crown ether. A dramatic blue-shift of the photo-isomerization spectrum was observed upon complexation, which could be well reproduced by ab initio calculations. Our results support that the observed changes in the photo-physical properties of the chromophore originate from the charge-solvation of its pyridinium moiety by the host cage...
November 30, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27851982/interdomain-electron-transfer-in-cellobiose-dehydrogenase-is-governed-by-surface-electrostatics
#17
Alan Kadek, Daniel Kavan, Julien Marcoux, Johann Stojko, Alfons K G Felice, Sarah Cianférani, Roland Ludwig, Petr Halada, Petr Man
BACKGROUND: Cellobiose dehydrogenase (CDH) is a fungal extracellular oxidoreductase which fuels lytic polysaccharide monooxygenase with electrons during cellulose degradation. Interdomain electron transfer between the flavin and cytochrome domain in CDH, preceding the electron flow to lytic polysaccharide monooxygenase, is known to be pH dependent, but the exact mechanism of this regulation has not been experimentally proven so far. METHODS: To investigate the structural aspects underlying the domain interaction in CDH, hydrogen/deuterium exchange (HDX-MS) with improved proteolytic setup (combination of nepenthesin-1 with rhizopuspepsin), native mass spectrometry with ion mobility and electrostatics calculations were used...
November 13, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27823666/modifier-assisted-differential-mobility-tandem-mass-spectrometry-method-for-detection-and-quantification-of-amphetamine-type-stimulants-in-urine
#18
Pai-Shan Chen, Shu-Hua Chen, Jung-Hsuan Chen, Wan-Yun Haung, Hsin-Tung Liu, Po-Hsin Kong, Olivia Hsu-Yuan Yang
An advantage of differential mobility spectrometry (DMS) is it provides an orthogonal mechanism to mass spectrometry (MS). The DMS-MS/MS detects analytes in the gas phase on the basis of differences in ion mobility in low and high electric fields, which makes DMS-MS/MS an alternative to chromatographic separation-MS. One drawback of DMS is its limited resolution and sensitivity, especially for detecting small molecules when using a nonpolar inert gas as the carrier gas. The present work has evaluated the effects on peak capacity of adding chemical modifiers to inert carrier gases (nitrogen, helium, argon and carbon dioxide)...
November 23, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27822705/structural-characterization-of-monomers-and-oligomers-of-d-amino-acid-containing-peptides-using-t-wave-ion-mobility-mass-spectrometry
#19
Xueqin Pang, Chenxi Jia, Zhengwei Chen, Lingjun Li
The D-residues are crucial to biological function of D-amino acid containing peptides (DAACPs). Previous ion mobility mass spectrometry (IM-MS) studies revealing oligomerization patterns of amyloid cascade demonstrated conversion from native soluble unstructured assembly to fibril ß-sheet oligomers, which has been implicated in amyloid diseases, such as Alzheimer's disease and type 2 diabetes. Although neuropeptides are typically present at very low concentrations in circulation, their local concentrations could be much higher in large dense core vesicles, forming dimers or oligomers...
November 7, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27812920/signatures-of-mechanically-interlocked-topology-of-lasso-peptides-by-ion-mobility-mass-spectrometry-lessons-from-a-collection-of-representatives
#20
Kevin Jeanne Dit Fouque, Hélène Lavanant, Séverine Zirah, Julian D Hegemann, Marcel Zimmermann, Mohamed A Marahiel, Sylvie Rebuffat, Carlos Afonso
Lasso peptides are characterized by a mechanically interlocked structure, where the C-terminal tail of the peptide is threaded and trapped within an N-terminal macrolactam ring. Their compact and stable structures have a significant impact on their biological and physical properties and make them highly interesting for drug development. Ion mobility - mass spectrometry (IM-MS) has shown to be effective to discriminate the lasso topology from their corresponding branched-cyclic topoisomers in which the C-terminal tail is unthreaded...
November 3, 2016: Journal of the American Society for Mass Spectrometry
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