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https://www.readbyqxmd.com/read/27903090/pediococcus-acidilactici-lab4-and-lactobacillus-plantarum-lab12-assimilate-cholesterol-and-modulate-abca1-cd36-npc1l1-and-scarb1-in-vitro
#1
F T Lim, S M Lim, K Ramasamy
There is growing interest in the use of probiotic lactic acid bacteria (LAB) for prevention of hypercholesterolaemia. This study assessed the cholesterol lowering ability of Pediococcus acidilactici LAB4 and Lactobacillus plantarum LAB12 in growth media. Both LAB yielded >98% (39.2 μg/ml) cholesterol lowering in growth media. Nile Red staining indicated direct assimilation of cholesterol by the LAB. The LAB were then explored for their prophylactic (pre-treatment of HT29 cells with LAB prior to cholesterol exposure) and biotherapeutic (treatment of HT29 cells with LAB after exposure to cholesterol) use against short and prolonged exposure of HT29 cells to cholesterol, respectively...
December 1, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27902765/loss-of-cathepsin-b-and-l-leads-to-lysosomal-dysfunction-npc-like-cholesterol-sequestration-and-accumulation-of-the-key-alzheimer-s-proteins
#2
Stjepko Cermak, Marko Kosicek, Aleksandra Mladenovic-Djordjevic, Kosara Smiljanic, Selma Kanazir, Silva Hecimovic
Proper function of lysosomes is particularly important in neurons, as they cannot dilute accumulated toxic molecules and aggregates by cell division. Thus, impairment of lysosomal function plays an important role in neuronal degeneration and in the pathogenesis of numerous neurodegenerative diseases. In this work we analyzed how inhibition and/or loss of the major lysosomal proteases, the cysteine cathepsins B and L (CtsB/L), affects lysosomal function, cholesterol metabolism and degradation of the key Alzheimer's disease (AD) proteins...
2016: PloS One
https://www.readbyqxmd.com/read/27890613/activation-of-ppar%C3%AE-does-not-contribute-to-macrophage-abca1-expression-and-abca1-mediated-cholesterol-efflux-to-apoai
#3
Meixiu Jiang, Xiaoju Li
Activation of macrophage ABCA1/G1 expression and cholesterol efflux is believed one of the mechanisms by which PPARγ inhibits atherosclerosis. PPARγ can also activate CD36 expression, a receptor for oxLDL, which may supply LXR ligands to activate LXR-ABCA1/G1 pathways. However, the controversial effects of PPARγ on ABCA1 expression have been reported. In this study, we used peritoneal macrophages isolated from wild type and CD36 deficient (CD36(-/-)) mice to clarify if PPARγ ligands can influence ABCA1 expression by CD36 function...
November 24, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27890580/lipid-free-apoa-i-structure-origins-of-model-diversity
#4
REVIEW
Michael N Oda
Apolipoprotein A-I (apoA-I) is a prominent member of the exchangeable apolipoprotein class of proteins, capable of transitioning between lipid-bound and lipid-free states. It is the primary structural and functional protein of high density lipoprotein (HDL). Lipid-free apoA-I is critical to de novo HDL formation as it is the preferred substrate of the lipid transporter, ATP Binding Cassette Transporter A1 (ABCA1) Remaley et al. (2001) [1]. Lipid-free apoA-I is an important element in reverse cholesterol transport and comprehension of its structure is a core issue in our understanding of cholesterol metabolism...
November 24, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27889764/high-uric-acid-activates-the-ros-ampk-pathway-impairs-cd68-expression-and-inhibits-oxldl-induced-foam-cell-formation-in-a-human-monocytic-cell-line-thp-1
#5
Chaohuan Luo, Xueke Lian, Liangli Hong, Jingfang Zou, Zhi Li, Yuzhang Zhu, Tianliang Huang, Yongneng Zhang, Yaqiu Hu, Huier Yuan, Tengfei Wen, Wanling Zhuang, Bozhi Cai, Xin Zhang, Ichiro Hisatome, Tetsuya Yamamoto, Jiexiong Huang, Jidong Cheng
BACKGROUND/AIMS: Hyperuricemia is part of the metabolic-syndrome cluster of abdominal obesity, impaired glucose tolerance, insulin resistance, dyslipidemia, and hypertension. Monocytes/macrophages are critical in the development of metabolic syndrome, including gout, obesity and atherosclerosis. However, how high uric acid (HUA) exposure affects monocyte/macrophage function remains unclear. In this study, we investigated the molecular mechanism of HUA exposure in monocytes/macrophages and its impact on oxidized low-density lipoprotein (oxLDL)-induced foam-cell formation in a human monocytic cell line, THP-1...
November 25, 2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27889409/bioactive-compound-screen-for-pharmacological-enhancers-of-apolipoprotein-e-in-primary-human-astrocytes
#6
Gina M Finan, Ronald Realubit, Sungkwon Chung, Dieter Lütjohann, Nan Wang, John R Cirrito, Charles Karan, Tae-Wan Kim
Pharmacological screening in physiologically relevant brain cells is crucial for identifying neuroactive compounds that better translate into in vivo biology and efficacious therapeutics. Pharmacological enhancement of apolipoprotein E (apoE), a cholesterol-transporting apolipoprotein, has been proposed as a promising therapeutic approach for Alzheimer's disease. Several nuclear receptor agonists were initially shown to increase brain apoE levels together with ATP-binding cassette transporter 1 (ABCA1), but their underlying mechanisms remain unclear...
November 18, 2016: Cell Chemical Biology
https://www.readbyqxmd.com/read/27881008/mir-33a-expression-sensitizes-lgr5-hcc-cscs-to-doxorubicin-via-abca1
#7
H Hou, Y Kang, Y Li, Y Zheng, G Ding, J Shang
Cancer stem cells (CSCs) are responsible for the unrestrained cell growth and chemo-resistance of malignant tumors. Reports about miR-33a in different type of cancer are limited, and it remains elusive whether there is a link between miR-33a and chemo-resistance of CSCs. Here we report that Lgr5+ hepatocellular carcinoma (HCC) cells from primary tissues and cell lines behave similarly to CSCs and are chemo-resistant to doxorubicin. Significantly, reduced miR-33a expression is associated with the chemo-resistance of Lgr5+ HCC-CSCs, accompanied by an overexpression of ABCA1 which is identified as target of miR-33a by mainly using miRNA luciferase assay and western-blotting...
November 24, 2016: Neoplasma
https://www.readbyqxmd.com/read/27871059/selective-p38%C3%AE-map-kinase-mapk14-inhibition-in-enzymatically-modified-ldl-stimulated-human-monocytes-implications-for-atherosclerosis
#8
Fei Cheng, Laura Twardowski, Sarah Fehr, Christoph Aner, Elke Schaeffeler, Thomas Joos, Thomas Knorpp, Bernhard Dorweiler, Stefan Laufer, Matthias Schwab, Michael Torzewski
The first ATP-competitive p38α MAPK/MAPK14 inhibitor with excellent in vivo efficacy and selectivity, skepinone-L, is now available. We investigated the impact of selective p38α MAPK/MAPK14 inhibition on enzymatically modified LDL (eLDL) stimulated human monocytes with its implications for atherosclerosis. Among the different p38 MAPK isoforms, p38α/MAPK14 was the predominantly expressed and activated isoform in isolated human peripheral blood monocytes. Moreover, eLDL colocalized with macrophages positive for p38α MAPK/MAPK14 in human carotid endarterectomy specimens...
November 8, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27869741/isoliquiritigenin-attenuates-atherogenesis-in-apolipoprotein-e-deficient-mice
#9
Fen Du, Quzhen Gesang, Jia Cao, Mei Qian, Li Ma, Dongfang Wu, Hong Yu
Isoliquiritigenin (ISL) exhibits antioxidation and anti-inflammation activity. We sought to investigate the effects and mechanism of ISL on the development of atherosclerotic lesions in apolipoprotein E-deficient (apoE(-/-)) mice. Firstly, we determined that ISL reduced the mRNA levels of inflammatory factors interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), and monocyte chemotactic protein-1 (MCP-1), while it increased the expression of several lipoprotein-related genes in peritoneal macrophages treated with lipopolysaccharide (LPS)...
November 18, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27862930/piperine-inhibits-abca1-degradation-and-promotes-cholesterol-efflux-from-thp-1-derived-macrophages
#10
Limei Wang, Veronika Palme, Susanne Rotter, Nicole Schilcher, Malsor Cukaj, Dongdong Wang, Angela Ladurner, Elke H Heiss, Herbert Stangl, Verena M Dirsch, Atanas G Atanasov
SCOPE: Increased macrophage cholesterol efflux is considered to have anti-atherosclerotic effect counteracting cardiovascular disease. The principle pungent ingredient of the fruits of Piper nigrum, piperine, is identified in this study as a cholesterol efflux (ChE) inducer in THP-1-derived macrophages, and mechanisms underlying this effect are explored. METHODS AND RESULTS: Without affecting cell viability, piperine concentration-dependently enhances ChE in THP-1-derived macrophages from 25 to 100 μM...
November 15, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27859593/placental-specific-overexpression-of-sflt-1-alters-trophoblast-differentiation-and-nutrient-transporter-expression-in-an-iugr-mouse-model
#11
E Kühnel, V Kleff, V Stojanovska, S Kaiser, R Waldschütz, F Herse, T Plösch, E Winterhager, A Gellhaus
Since it is known that placental overexpression of the human anti-angiogenic molecule sFlt-1, the main candidate in the progression of preeclampsia, lead to intrauterine growth restriction (IUGR) in mice by lentiviral transduction of mouse blastocysts, we hypothesize that sFlt-1 influence placental morphology and physiology resulting in fetal IUGR. We therefore examined the effect of sFlt-1 on placental morphology and physiology at embryonic day 18.5 with histologic and morphometric analyses, transcript analyses, immunoblotting, and methylation studies...
November 9, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27853448/a-novel-mutation-in-abca1-gene-causing-tangier-disease-in-an-italian-family-with-uncommon-neurological-presentation
#12
Marco Ceccanti, Chiara Cambieri, Vittorio Frasca, Emanuela Onesti, Antonella Biasiotta, Carla Giordano, Sabina M Bruno, Giancarlo Testino, Marco Lucarelli, Marcello Arca, Maurizio Inghilleri
Tangier disease is an autosomal recessive disorder characterized by severe reduction in high-density lipoprotein cholesterol and peripheral lipid storage. We describe a family with c.5094C > A p.Tyr1698* mutation in the ABCA1 gene, clinically characterized by syringomyelic-like anesthesia, demyelinating multineuropathy, and reduction in intraepidermal small fibers innervation. In the proband patient, cardiac involvement determined a myocardial infarction; lipid storage was demonstrated in gut, cornea, and aortic wall...
2016: Frontiers in Neurology
https://www.readbyqxmd.com/read/27845788/effects-of-pomegranate-peel-polyphenols-on-lipid-accumulation-and-cholesterol-metabolic-transformation-in-l-02-human-hepatic-cells-via-the-ppar%C3%AE-abca1-cyp7a1-pathway
#13
Ou Lv, Lifang Wang, Jianke Li, Qianqian Ma, Wei Zhao
To study the effect of pomegranate peel polyphenols on lipid accumulation and cholesterol metabolic transformation in human hepatic cells, purified pomegranate peel polyphenols (PPPs), their main component, punicalagin (PC), and the metabolite of PC, pomegranate ellagic acid (PEA), were chosen as the polyphenols to be tested. At the same time the human hepatocyte cell line L-02 was selected as the experimental cell and a model of steatotic L-02 hepatocytes in vitro was constructed in this paper. The results showed that PPPs, PC and PEA in different concentrations could decrease the total cholesterol (TC) content and increase the total bile acid (TBA) content, and so possess a lipid-lowering effect...
November 15, 2016: Food & Function
https://www.readbyqxmd.com/read/27829662/high-glucose-upregulates-bace1-mediated-a%C3%AE-production-through-ros-dependent-hif-1%C3%AE-and-lxr%C3%AE-abca1-regulated-lipid-raft-reorganization-in-sk-n-mc-cells
#14
Hyun Jik Lee, Jung Min Ryu, Young Hyun Jung, Sei-Jung Lee, Jeong Yeon Kim, Sang Hun Lee, In Koo Hwang, Je Kyung Seong, Ho Jae Han
There is an accumulation of evidence indicating that the risk of Alzheimer's disease is associated with diabetes mellitus, an indicator of high glucose concentrations in blood plasma. This study investigated the effect of high glucose on BACE1 expression and amyloidogenesis in vivo, and we present details of the mechanism associated with those effects. Our results, using ZLC and ZDF rat models, showed that ZDF rats have high levels of amyloid-beta (Aβ), phosphorylated tau, BACE1, and APP-C99. In vitro result with mouse hippocampal neuron and SK-N-MC, high glucose stimulated Aβ secretion and apoptosis in a dose-dependent manner...
November 10, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27824936/differential-effects-of-apoe4-and-activation-of-abca1-on-brain-and-plasma-lipoproteins
#15
Anat Boehm-Cagan, Roni Bar, Dror Harats, Aviv Shaish, Hana Levkovitz, John K Bielicki, Jan O Johansson, Daniel M Michaelson
Apolipoprotein E4 (apoE4), the leading genetic risk factor for Alzheimer's disease (AD), is less lipidated compared to the most common and AD-benign allele, apoE3. We have recently shown that i.p. injections of the ATP-binding cassette A1 (ABCA1) agonist peptide CS-6253 to apoE mice reverse the hypolipidation of apoE4 and the associated brain pathology and behavioral deficits. While in the brain apoE is the main cholesterol transporter, in the periphery apoE and apoA-I both serve as the major cholesterol transporters...
2016: PloS One
https://www.readbyqxmd.com/read/27818935/intestinal-crebh-overexpression-prevents-high-cholesterol-diet-induced-hypercholesterolemia-by-reducing-npc1l1-expression
#16
Takuya Kikuchi, Kana Orihara, Fusaka Oikawa, Song-Iee Han, Motoko Kuba, Kanako Okuda, Aoi Satoh, Yoshinori Osaki, Yoshinori Takeuchi, Yuichi Aita, Takashi Matsuzaka, Hitoshi Iwasaki, Shigeru Yatoh, Motohiro Sekiya, Naoya Yahagi, Hiroaki Suzuki, Hirohito Sone, Yoshimi Nakagawa, Nobuhiro Yamada, Hitoshi Shimano
OBJECTIVE: The transcription factor cyclic AMP-responsive element-binding protein H (CREBH, encoded by Creb3l3) is highly expressed in the liver and small intestine. Hepatic CREBH contributes to glucose and triglyceride metabolism by regulating fibroblast growth factor 21 (Fgf21) expression. However, the intestinal CREBH function remains unknown. METHODS: To investigate the influence of intestinal CREBH on cholesterol metabolism, we compared plasma, bile, fecal, and tissue cholesterol levels between wild-type (WT) mice and mice overexpressing active human CREBH mainly in the small intestine (CREBH Tg mice) under different dietary conditions...
November 2016: Molecular Metabolism
https://www.readbyqxmd.com/read/27815075/gly-14-humanin-inhibits-ox-ldl-uptake-and-stimulates-cholesterol-efflux-in-macrophage-derived-foam-cells
#17
Wa-Wa Zhu, Shu-Rong Wang, Zhi-Hua Liu, Yong-Jun Cao, Fen Wang, Jing Wang, Chun-Feng Liu, Ying Xie, Yan-Lin Zhang
Foam cell formation, which is caused by imbalanced cholesterol influx and efflux by macrophages, plays a vital role in the occurrence and development of atherosclerosis. Humanin (HN), a mitochondria-derived peptide, can prevent the production of reactive oxygen species and death of human aortic endothelial cells exposed to oxidized low-density lipoprotein (ox-LDL) and has a protective effect on patients with in early atherosclerosis. However, the effects of HN on the regulation of cholesterol metabolism in RAW 264...
November 1, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27814978/identification-of-a-novel-lipid-binding-motif-in-apolipoprotein-b-by-the-analysis-of-hydrophobic-cluster-domains
#18
Scott M Gordon, Mohsen Pourmousa, Maureen Sampson, Denis Sviridov, Rafique Islam, B Scott Perrin, Georgina Kemeh, Richard W Pastor, Alan T Remaley
Apolipoprotein B (apoB) is a large amphipathic protein that is the structural scaffold for the formation of several classes of lipoproteins involved in lipid transport throughout the body. The goal of the present study was to identify specific domains in the apoB sequence that contribute to its lipid binding properties. A sequence analysis algorithm was developed to identify stretches of hydrophobic amino acids devoid of charged amino acids, which are referred to as hydrophobic cluster domains (HCDs). This analysis identified 78 HCDs in apoB with hydrophobic stretches ranging from 6 to 26 residues...
November 1, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27807992/radiation-induced-surge-of-macrophage-foam-cell-formation-oxidative-damage-and-cytokine-release-is-attenuated-by-a-nanoformulation-of-curcumin
#19
Behrooz Soltani, Narges Bodaghabadi, Nasser Ghaemi, Majid Sadeghizadeh
PURPOSE: We examined the potential of a dendrosomal nanoformulation of curcumin (DNC) for intervention of ionizing radiation (IR)-induced damage (particularly leading to atherosclerosis), employing an irradiated THP-1 macrophage model. MATERIALS AND METHODS: Differentiated THP-1 macrophages were irradiated and treated with curcumin or DNC nanoformulation (and oxidized low density lipoprotein, ox-LDL, to promote foam cells). Chemical, biochemical, and genetics tools including viability and apoptosis, multiple ELISA, real-time PCR, Western blotting, enzyme activity, and fluorimetry assays were employed to illustrate IR damage as well as the DNC intervention potential...
November 3, 2016: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27787652/transcriptional-control-of-apoptotic-cell-clearance-by-macrophage-nuclear-receptors
#20
Tamás Röszer
Apoptotic cell clearance by macrophages is key for normal tissue development and homeostasis. Nuclear receptors, such as peroxisome proliferator activated receptors (PPARs), liver X receptor (LXR), retinoic acid receptor (RAR), retinoid X receptor (RXR) and glucocorticoid receptor (GR) orchestrate this vital process. The underlying mechanism involves the transcriptional control of key genes of apoptotic cell recognition and internalization, such as Cd36, Mertk, Axl, C1qa, Tgm2, Abca1. In addition, apoptotic cell uptake leads to M2 activation of macrophages, and this process is also controlled at the gene transcription level by nuclear receptors...
October 27, 2016: Apoptosis: An International Journal on Programmed Cell Death
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