Taotao Li, Changrong Ge, Alexander Krämer, Outi Sareila, Monica Leu Agelii, Linda Johansson, Kristina Forslind, Erik Lönnblom, Min Yang, Bingze Xu, Qixing Li, Lei Cheng, Göran Bergström, Gonzalo Fernandez, Alf Kastbom, Solbritt Rantapää-Dahlqvist, Inger Gjertsson, Rikard Holmdahl
OBJECTIVES: To identify the arthritogenic B cell epitopes of glucose-6-phosphate isomerase (GPI) and their association with rheumatoid arthritis (RA). METHODS: IgG response towards a library of GPI peptides in patients with early RA, pre-symptomatic individuals and population controls, as well as in mice, were tested by bead-based multiplex immunoassays and ELISA. Monoclonal IgG were generated, and the binding specificity and affinity were determined by ELISA, gel size exclusion chromatography, surface plasma resonance and X-ray crystallography...
March 1, 2023: Annals of the Rheumatic Diseases