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https://www.readbyqxmd.com/read/28780857/barcode-dna-mediated-signal-amplifying-strategy-for-ultrasensitive-biomolecular-detection-on-matrix-assisted-laser-desorption-ionization-time-of-flight-maldi-tof-mass-spectrometry
#1
Raheel Ahmad, Hyowon Jang, Bhagwan S Batule, Hyun Gyu Park
We have devised a barcode DNA-mediated signal amplifying strategy for ultrasensitive biomolecular detection by utilizing matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). As a model target, thrombin was first captured by specific aptamer15 functionalized on magnetic beads (MBs-apt15) and sandwiched through the simultaneous interaction with gold nanoparticles modified with another specific aptamer29 and barcode DNA molecules (apt29-AuNPs-bcDNAs). The sandwiched complex was collected by convenient magnetic separation and then treated with potassium cyanide (KCN) to dissolve the gold nanoparticles (AuNPs) and consequently release the barcode DNA molecules (bcDNAs), which were then again magnetically separated and analyzed by using MALDI-TOF MS...
August 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28754902/effect-of-sodium-treatment-on-the-performance-of-electrostatic-spray-assisted-vapour-deposited-copper-poor-cu-in-ga-s-se-2-solar-cells
#2
Mingqing Wang, Md Anower Hossain, Kwang-Leong Choy
In our work, eco-friendly, non-vacuum and low cost Electrostatic Spray Assisted Vapour Deposition (ESAVD) method has been used to produce Cu(In,Ga)(S,Se)2 (CIGS) solar cells. Copper (Cu) deficient (Cu/In + Ga = 0.76) CIGS films were designed to avoid the rather dangerous KCN treatment step for the removal of conductive minor phases of Cu2S/Cu2Se. A simple sodium (Na) treatment method was used to modify the morphology and electronic properties of the absorber and it clearly improved the solar cell performance...
July 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28749896/elimination-of-anterior-corneal-steepening-with-descemet-membrane-endothelial-keratoplasty-in-a-patient-with-fuchs-dystrophy-and-keratoconus-implications-for-iol-calculation
#3
Reena Gupta, Ruta Kinderyte, Deborah S Jacobs, Ula V Jurkunas
PURPOSE: To report a case of coexistent Fuchs endothelial corneal dystrophy (FECD) and keratoconus (KCN) in which there was normalization of corneal topography after Descemet membrane endothelial keratoplasty (DMEK). METHODS: Retrospective medical record review. RESULTS: Preoperative findings revealed a best-corrected visual acuity of 20/40 with -1.00 - 2.50 × 147, topographic maximum keratometry of 50.8 D with inferior steeping, and confluent guttae in the left eye...
July 26, 2017: Cornea
https://www.readbyqxmd.com/read/28735400/zymographic-method-for-distinguishing-different-classes-of-superoxide-dismutases-in-plants
#4
Ashwini R Jamdhade, Ramanjulu Sunkar, Vandana K Hivrale
In plants, especially in chloroplasts, superoxide radical is generated when an electron is transferred to dimolecular O2 due to decreased activity of Photosystem I. The superoxide (O2(-)) radical accumulation is more rampant in plants exposed to abiotic stresses due to oxidation of photosystem components. Excessive superoxide radical accumulation will lead to oxidative damage to the cellular macromolecules. The ubiquitous superoxide dismutases (SODs) represent critical enzymatic antioxidant system present in cells, which can catalyze the disproportion of superoxide (O2(-)) radical rapidly into hydrogen peroxide (H2O2) and molecular oxygen...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28711978/the-activity-of-the-antioxidant-defense-system-of-the-weed-species-senna-obtusifolia-l-and-its-resistance-to-allelochemical-stress
#5
Érica Marusa Pergo Coelho, Mauro Cezar Barbosa, Márcio Shigueaki Mito, Gislaine Cristiane Mantovanelli, Rubem Silvério Oliveira, Emy Luiza Ishii-Iwamoto
Senna obtusifolia L., a common weed in the tropical and subtropical regions of the world, is able to germinate under adverse environmental conditions, suggesting that this species has efficient stress-adaptation strategies. The aims of the present work were to examine the energy metabolism and the antioxidant defense system of the Senna obtusifolia L. during seed germination and initial growth, and the responses to allelochemical-induced stress. Respiratory activity, the activities of alcohol dehydrogenase (ADH), superoxide dismutase (SOD), catalase (CAT),guaicol peroxidase (POD), ascorbate peroxidase (APX), glutathione reductase (GR), lipoxygenase (LOX) and the content of malondialdehyde (MDA) and glutathione (GSSG and GSH) were measured...
July 15, 2017: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/28688894/a-review-of-keratoconus-diagnosis-pathophysiology-and-genetics
#6
V Mas-Tur, C MacGregor, R Jayaswal, D P S O'Brart, N J R Maycock
We discuss new approaches to the early detection of keratoconus (KCN) and recent investigations regarding the nature of its pathophysiology. We review the current evidence for its complex genetics and evaluate the presently identified genes/loci and potential candidate gene/loci. In addition, we highlight current research methodologies that may be used to further elucidate the pathogenesis of KCN.
July 5, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/28634393/a-small-molecule-ds44170716-inhibits-ca-2-induced-mitochondrial-permeability-transition
#7
Naohiro Kon, Atsushi Satoh, Naoki Miyoshi
Mitochondria are involved in a variety of physiological and pathological processes. Ca(2+) uptake is one of the important functions of the organelle for maintenance of cellular Ca(2+) homeostasis. In pathological conditions such as ischemia reperfusion injury, Ca(2+) overload into mitochondria induces mitochondrial permeability transition (MPT), a critical step for cell death. Because inhibition of MPT is a promising approach to protecting cells and organs, it is important for drug discovery to identify novel chemicals or mechanisms to inhibit MPT...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28623432/identification-and-expression-analysis-of-cys-a1-cys-c1-nit4-genes-in-rice-seedlings-exposed-to-cyanide
#8
Xiao-Zhang Yu, Yu-Juan Lin, Chun-Jiao Lu, Xue-Hong Zhang
Involvement of genes (CYS-A1, CYS-C1 and NIT4) encoded with cysteine synthase, β-cyanoalanine synthase, nitrilase and cyanide metabolisms are evident in Arabidopsis. In the present study, identifications of CYS-A1, CYS-C1 and NIT4, predictions of conserved motifs, and constructions of phylogenetic relationships, based on their amino acid sequences in rice, were conducted. In order to elucidate the transcriptional responses of these cyanide-degrading genes, two candidate homologues were selected for each gene to test their expression changes upon exposure to exogenous KCN in rice seedlings using RT-PCR...
June 16, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28552364/rna-seq-analysis-of-the-role-of-calcium-chloride-stress-and-electron-transport-in-mitochondria-for-malachite-green-decolorization-by-aspergillus-niger
#9
Ola M Gomaa, Nabila S Selim, Josephine Wee, John E Linz
Aspergillus niger was previously demonstrated to decolorize the commercial dye malachite green (MG) and this process was enhanced under calcium chloride (CaCl2) treatment. Previous data also suggested that the decolorization process is related to mitochondrial cytochrome c. In the current work, we analyzed in depth the specific relationship between CaCl2 treatment and MG decolorization. Gene expression analysis (RNA Seq) using Next Generation Sequencing (NGS) revealed up-regulation of 28 genes that are directly or indirectly associated with stress response functions as early as 30min of CaCl2 treatment; these data further strengthen our previous findings that CaCl2 treatment induces a stress response in A...
May 24, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28534231/comparison-of-outcomes-and-complications-of-deep-anterior-lamellar-keratoplasty-and-penetrating-keratoplasty-performed-in-a-large-group-of-patients-with-keratoconus
#10
Ashbala Khattak, Fouad R Nakhli, Khaled M Al-Arfaj, Abdullah A Cheema
PURPOSE: To compare visual acuity, refraction, topography, and complications of deep anterior lamellar keratoplasty (DALK) and penetrating keratoplasty (PKP) performed in patients with keratoconus (KCN). METHODS: In this retrospective interventional non-randomized clinical study, patients with moderate to severe KCN that underwent either PKP or DALK for optical corrections were enrolled. The final outcome analysis was performed after complete suture removal for both groups of patients...
May 22, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28497061/mining-of-microbial-genomes-for-the-novel-sources-of-nitrilases
#11
Nikhil Sharma, Neerja Thakur, Tilak Raj, Savitri, Tek Chand Bhalla
Next-generation DNA sequencing (NGS) has made it feasible to sequence large number of microbial genomes and advancements in computational biology have opened enormous opportunities to mine genome sequence data for novel genes and enzymes or their sources. In the present communication in silico mining of microbial genomes has been carried out to find novel sources of nitrilases. The sequences selected were analyzed for homology and considered for designing motifs. The manually designed motifs based on amino acid sequences of nitrilases were used to screen 2000 microbial genomes (translated to proteomes)...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28435217/patient-selection-for-corneal-collagen-cross-linking-an-updated-review
#12
REVIEW
Virgilio Galvis, Alejandro Tello, Alvaro I Ortiz, Luis C Escaf
Corneal cross-linking (CXL) is an option that in the last decade has demonstrated its efficacy and safety in halting the progression of keratoconus (KCN) and other corneal ectasias. Its indication has been extended beyond the classic definition that required evidence of KCN progression, especially in the presence of some risk factors for a possible progression (particularly the younger age). However, the results can be still somewhat variable today. There are several protocols, each with its own advantages and disadvantages...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28432454/cobalt-tetradehydrocorrins-coordinated-by-imidazolate-like-histidine-in-the-heme-pocket-of-horseradish-peroxidase
#13
Koji Oohora, Ning Tang, Yoshitsugu Morita, Takashi Hayashi
Horseradish peroxidase was reconstituted with cobalt tetradehydrocorrin, rHRP(Co(TDHC)), as a structural analog of cobalamin coordinated with an imidazolate-like His residue, which is generally seen in native enzymes. In contrast to the previously reported cobalt tetradehydrocorrin-reconstituted myoglobin, rMb(Co(TDHC)), the HRP matrix was expected to provide strong axial ligation by His170 which has imidazolate character. rHRP(Co(II)(TDHC)) was characterized by EPR and its reaction with reductants indicates a negative shift of its redox potential compared to rMb(Co(TDHC))...
April 21, 2017: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/28424712/a-functional-approach-towards-understanding-the-role-of-the-mitochondrial-respiratory-chain-in-an-endomycorrhizal-symbiosis
#14
Louis Mercy, Eva Lucic-Mercy, Amaia Nogales, Areg Poghosyan, Carolin Schneider, Birgit Arnholdt-Schmitt
Arbuscular mycorrhizal fungi (AMF) are crucial components of fertile soils, able to provide several ecosystem services for crop production. Current economic, social and legislative contexts should drive the so-called "second green revolution" by better exploiting these beneficial microorganisms. Many challenges still need to be overcome to better understand the mycorrhizal symbiosis, among which (i) the biotrophic nature of AMF, constraining their production, while (ii) phosphate acts as a limiting factor for the optimal mycorrhizal inoculum application and effectiveness...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28382074/inhibiting-cytochrome-c-oxidase-leads-to-alleviated-ischemia-reperfusion-injury
#15
Zhaoyun Yang, Zhongxin Duan, Tian Yu, Junmei Xu, Lei Liu
BACKGROUND AND OBJECTIVES: The overall purpose of this study was to investigate the role of cytochrome C oxidase (CcO) in preventing ischemia reperfusion-induced cardiac injury through gaseous signaling molecule pathways. MATERIALS AND METHODS: We used CcO inhibitor, potassium cyanide (KCN) to mimic the pre-treatment of gaseous signaling molecules in a global ischemia/reperfusion (IR) injury model in rats. Intracellular reactive oxygen species (ROS) was determined by measuring mitochondrial H2O2 and mitochondrial complex activity...
March 2017: Korean Circulation Journal
https://www.readbyqxmd.com/read/28328983/novel-keyword-co-occurrence-network-based-methods-to-foster-systematic-reviews-of-scientific-literature
#16
Srinivasan Radhakrishnan, Serkan Erbis, Jacqueline A Isaacs, Sagar Kamarthi
Systematic reviews of scientific literature are important for mapping the existing state of research and highlighting further growth channels in a field of study, but systematic reviews are inherently tedious, time consuming, and manual in nature. In recent years, keyword co-occurrence networks (KCNs) are exploited for knowledge mapping. In a KCN, each keyword is represented as a node and each co-occurrence of a pair of words is represented as a link. The number of times that a pair of words co-occurs in multiple articles constitutes the weight of the link connecting the pair...
2017: PloS One
https://www.readbyqxmd.com/read/28318118/distinct-expression-of-nk2-homeobox-1-nkx2-1-and-goblet-cell-hyperplasia-in-nasal-polyps-with-different-endotypes
#17
Jintao Du, Luo Ba, Bo Li, Feng Liu, Xianting Hu, Jie Zhang, Yafeng Liu, Junming Xian, Sixi Liu, Huabin Li
BACKGROUND: Decreased expression of airway epithelial-specific transcription factor NK2 homeobox 1 (NKX2-1) was associated with allergic inflammation in asthma patients. However, the expression and role of NKX2-1 in nasal polyps (NPs) with different endotypes were undefined yet. METHODS: We examined the expression of key cytokines (interleukin [IL]-4 IL-5, IL-13, and IL-17A, etc.) and NKX2-1 in NPs with different endotypes and control tissues by immunohistochemistry staining, qualitative polymerase chain reaction (qPCR), enzyme-linked immunosorbent assay (ELISA), and Western blot analysis...
July 2017: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/28299936/development-of-decarboxylative-cyanation-reactions-for-c-13-c-14-carboxylic-acid-labeling-using-an-electrophilic-cyanating-reagent
#18
Fengbin Song, Rhys Salter, Lu Chen
Degradation-reconstruction approaches for isotope labeling synthesis have been known for their remarkable efficiency, but applications are scarce due to some fundamental limitations of the chemistries developed to date. The decarboxylative cyanation reaction, as a degradation-reconstruction approach, is especially useful in rapid carboxylic acid carbon isotope labeling, however development toward its application as a widespread technique has stalled at the early stages due to numerous limitations which include somewhat narrow applicability...
March 16, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28286097/effect-of-the-periodic-properties-of-toxic-stress-on-the-oscillatory-behaviour-of-glycolysis-in-yeast-evidence-of-a-toxic-effect-frequency
#19
COMPARATIVE STUDY
C André, F Gagné
Starving and nondividing yeast cells induce changes in the electron donor nicotinamide adenine dinucleotide (NADH) levels in a cyclic and wave-like manner for over 90min. Yeast suspensions were used to examine the toxic effects of contaminants on the cyclic behaviour of metabolite changes during anaerobic glycolysis. The cyclic behaviour NADH levels in yeast cell suspensions starved for 2 to 5h was studied after the addition of 10mM glucose for 5min followed by 10mM KCN to block aerobic glycolysis. The effects of three toxic elements (CuSO4, silver nanoparticles-nAg, and GdCl3), known for their potential to alter glycolsysis, on NADH levels over time were examined during the 3-h starvation step...
June 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28279900/electrochemical-oxidation-of-cyanide-on-3d-ti-ruo2-anode-using-a-filter-press-electrolyzer
#20
Tzayam Pérez, Rosa L López, José L Nava, Isabel Lázaro, Guillermo Velasco, Roel Cruz, Israel Rodríguez
The novelty of this communication lies in the use of a Ti-RuO2 anode which has not been tested for the oxidation of free cyanide in alkaline media at concentrations similar to those found in wastewater from the Merrill Crowe process (100 mg L(-1) KCN and pH 11), which is typically used for the recovery of gold and silver. The anode was prepared by the Pechini method and characterized by SEM. Linear sweep voltammetries on a Ti-RuO2 rotating disk electrode (RDE) confirmed that cyanide is oxidized at 0.45 < E < 1...
June 2017: Chemosphere
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