keyword
MENU ▼
Read by QxMD icon Read
search

Real time pcr intrapartum gbs

keyword
https://www.readbyqxmd.com/read/27378534/-usefulness-of-a-rapid-intrapartum-real-time-pcr-assay-in-comparison-with-the-group-b-streptococcus-culture-screening-at-the-end-of-pregnancy-in-pregnant-women
#1
M Defez, F Khizar, M Maurin, F Biot, J-C Pons, F Sergent
OBJECTIVES: The objectives were to evaluate and compare the diagnostic accuracy of a rapid real-time PCR assay at the onset of labor with those of the current antenatal culture-based test at 34-38 weeks gestation for group B Streptococcus (GBS) screening. MATERIALS AND METHODS: A prospective study including all pregnant women admitted for delivery after a 34-week gestation period was conducted in October 2012 at the Grenoble University Hospital Centre. A first culture-based GBS screening test was performed between 34 and 38 weeks of gestation followed by a second screening test at the onset of labor, using a real-time PCR Assay and a culture-based method (gold standard) in order to calculate the diagnostic accuracy...
July 1, 2016: Journal de Gynécologie, Obstétrique et Biologie de la Reproduction
https://www.readbyqxmd.com/read/26237371/influence-of-intrapartum-antibiotic-prophylaxis-for-group-b-streptococcus-on-gut-microbiota-in-the-first-month-of-life
#2
Luigi Corvaglia, Giacomo Tonti, Silvia Martini, Arianna Aceti, Giuseppe Mazzola, Irene Aloisio, Diana Di Gioia, Giacomo Faldella
OBJECTIVES: The effect of intrapartum antibiotic prophylaxis (IAP) for group B Streptococcus (GBS) on bacterial colonization of the infant's gut has not been investigated extensively. We aimed to evaluate the effect of IAP on gut microbiota in healthy term infants, also exploring the influence of type of feeding. METHODS: Healthy term infants, whose mothers had been screened for GBS in late gestation, were divided into 2 groups: infants born to GBS-positive mothers who had received IAP versus controls...
February 2016: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/26153188/culture-and-real-time-pcr-based-maternal-screening-and-antibiotic-susceptibility-for-group-b-streptococcus-an-iranian-experience
#3
Gholamreza Goudarzi, Masoumeh Ghafarzadeh, Pegah Shakib, Khatereh Anbari
INTRODUCTION: Vertical Transmission of group B streptococcus (GBS) from a vagina colonized mother to her infant upon rupture of membranes (ROM) or after the onset of labor can cause life-threatening infections in newborn. Although intrapartum antibiotic prophylaxis (IAP) can significantly decrease neonatal GBS diseases, this issue has potentiated the emergence of antibiotic resistance strains. Our study examined the colonization rate of GBS using real-time PCR and culture methods, and trends in antibiotic resistance of GBS isolates obtained from pregnant women in Khorramabad, Iran...
2015: Global Journal of Health Science
https://www.readbyqxmd.com/read/25471089/intrapartum-group-b-streptococcus-screening-using-real-time-polymerase-chain-reaction-in-japanese-population
#4
Kei Tanaka, Mitsutoshi Iwashita, Miho Matsushima, Yuichi Wachi, Tomoko Izawa, Keiji Sakai, Yoichi Kobayashi
OBJECTIVE: The objective of this study was to analyze the diagnostic accuracy of a commercial real-time polymerase chain reaction (PCR) assay for group B streptococcus (GBS) colonization status and to compare results of the intrapartum PCR with the antepartum conventional GBS culture in Japanese pregnant women. METHODS: This prospective observational study enrolled Japanese pregnant women at 35-37 weeks' gestation. Paired recto-vaginal swabs were obtained for PCR and conventional culture, both at 35-37 weeks' gestation and at admission for delivery...
2016: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/25287153/direct-identification-of-streptococcus-agalactiae-and-capsular-type-by-real-time-pcr-in-vaginal-swabs-from-pregnant-women
#5
Miyuki Morozumi, Naoko Chiba, Yuko Igarashi, Naoki Mitsuhashi, Takeaki Wajima, Satoshi Iwata, Kimiko Ubukata
Most group B streptococcus (GBS) infections in newborns are with capsular type Ia, Ib, or III. To prevent these infections more effectively, we developed a real-time PCR method to simultaneously detect GBS species and identify these 3 capsular types in vaginal swab samples from women at 36-39 weeks of gestation. DNA to be detected included those of the dltS gene (encoding a histidine kinase specific to GBS) and cps genes encoding capsular types. PCR sensitivity was 10 CFU/well for a 33-35 threshold cycle. Results were obtained within 2 h...
January 2015: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/25197396/successful-off-label-use-of-the-cepheid-xpert-gbs-in-a-late-onset-neonatal-meningitis-by-streptococcus-agalactiae
#6
Vincenzo Savini, Roberta Marrollo, Eleonora Coclite, Paola Fusilli, Carmine D'Incecco, Paolo Fazii
We report the case of a late-onset neonatal meningitis by Streptococcus agalactiae (group B Streptococcus - GBS) that was diagnosed with a latex agglutination assay (on cerebrospinal fluid, CSF), as well as by using, for the first time, Xpert GBS (Cepheid, US) on CSF. Due to empirical antibiotics given before sampling, both CSF and blood culture were negative, so the abovementioned diagnostics was crucial. Moreover, the Xpert GBS assay, performed according to an off-label, modified protocol (the system is designed for GBS-carriage intrapartum screening, based on a completely automated real time-Polymerase Chain Reaction) quickly detected the organism's genome target...
2014: International Journal of Clinical and Experimental Pathology
https://www.readbyqxmd.com/read/24687755/influence-of-intrapartum-antibiotic-prophylaxis-against-group-b-streptococcus-on-the-early-newborn-gut-composition-and-evaluation-of-the-anti-streptococcus-activity-of-bifidobacterium-strains
#7
Irene Aloisio, Giuseppe Mazzola, Luigi Tommaso Corvaglia, Giacomo Tonti, Giacomo Faldella, Bruno Biavati, Diana Di Gioia
Several factors are known to influence the early colonization of the gut in newborns. Among them, the use of antibiotics on the mother during labor, referred to as intrapartum antibiotic prophylaxis (IAP), has scarcely been investigated, although this practice is routinely used in group B Streptococcus (GBS)-positive women. This work is therefore aimed at verifying whether IAP can influence the main microbial groups of the newborn gut microbiota at an early stage of microbial establishment. Fifty-two newborns were recruited: 26 born by mothers negative to GBS (control group) and 26 by mothers positive to GBS and subjected to IAP with ampicillin (IAP group)...
July 2014: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/23795754/real-time-pcr-assay-in-the-delivery-suite-for-determination-of-group-b-streptococcal-colonization-in-a-setting-with-risk-based-antibiotic-prophylaxis
#8
RANDOMIZED CONTROLLED TRIAL
Stellan Håkansson, Karin Källén, Maria Bullarbo, Per-Åke Holmgren, Katarina Bremme, Asa Larsson, Margareta Norman, Håkan Norén, Catharina Ortmark-Wrede, Karin Pettersson, Sissel Saltvedt, Birgitta Sondell, Magdalena Tokarska, Anna von Vultee, Bo Jacobsson
OBJECTIVE: Intrapartum antibiotic prophylaxis (IAP) reduces the incidence of neonatal early onset group B streptococcal infections. The present study investigated if an automated PCR-assay, used bedside by the labor ward personnel was manageable and could decrease the use of IAP in a setting with a risk-based IAP strategy. METHODS: The study comprises two phases. Phase 1 was a multicenter, randomized, controlled trial. Women with selected risk-factors were allocated either to PCR-IAP (prophylaxis given if positive or indeterminate) or IAP...
March 2014: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/23390279/evaluation-of-the-cepheid-xpert-gbs-assay-for-rapid-detection-of-group-b-streptococci-in-amniotic-fluids-from-pregnant-women-with-premature-rupture-of-membranes
#9
COMPARATIVE STUDY
Nadege Bourgeois-Nicolaos, Anne-Gael Cordier, Christelle Guillet-Caruba, François Casanova, Alexandra Benachi, Florence Doucet-Populaire
The Xpert GBS real-time PCR assay for the detection of group B streptococci (GBS) in antepartum screening samples was evaluated on amniotic fluid samples collected from 139 women with premature rupture of membrane at term. When any intrapartum positive result from the Xpert GBS or culture was considered a true positive, the sensitivities of the Xpert GBS and culture were 92.3% and 84.6%, respectively. This assay could enhance exact identification of candidates for intrapartum antibiotic prophylaxis.
April 2013: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/21396547/evaluation-of-the-xpert%C3%A2-group-b-streptococcus-real-time-polymerase-chain-reaction-assay-compared-to-strepb-carrot-broth%C3%A2-for-the-rapid-intrapartum-detection-of-group-b-streptococcus-colonization
#10
COMPARATIVE STUDY
Deirdre Leigh Church, Heather Baxter, Tracie Lloyd, Beverley Miller, Daniel B Gregson
Xpert group B streptococcus (GBS) was compared to StrepB Carrot Broth™ (SCB) for the detection of intrapartum GBS colonization by dually collecting vaginal/rectal swabs from 231 women. Xpert GBS detected all of the cases (45, 19.5%), but 4 were missed by SCB. A rapid Xpert GBS service for women in labor would increase costs by ∼$55.000 per annum in our region.
April 2011: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/21051844/development-of-a-rapid-pcr-assay-for-screening-of-maternal-colonization-by-group-b-streptococcus-and-neonatal-invasive-escherichia-coli-during-labor
#11
Begoña Martínez de Tejada, Catalin M Stan, Michel Boulvain, Gesuele Renzi, Patrice François, Olivier Irion, Jacques Schrenzel
OBJECTIVE: Group B Streptococcus (GBS) and Escherichiacoli(E. coli) are the leading causes of early-onset neonatal disease (EOD). Intrapartum antibiotic prophylaxis of GBS-colonized women decreases vertical transmission and EOD due to GBS. Nevertheless, no intervention has been developed to reduce the risk of EOD related to E. coli. Timely and accurate identification of colonized mothers is necessary to implement preventive strategies against neonatal sepsis. To screen for colonization during labor, we developed a real-time PCR assay for the simultaneous detection of GBS and neonatal invasive strains of E...
2010: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/20592137/real-time-pcr-assay-provides-reliable-assessment-of-intrapartum-carriage-of-group-b-streptococcus
#12
COMPARATIVE STUDY
Michelle J Alfa, Shadi Sepehri, Pat De Gagne, Michael Helawa, Gunwat Sandhu, Godfrey K M Harding
The objective of this study was to determine the reliability of the real-time PCR assay for determining the group B Streptococcus (GBS) status of women in labor. In this prospective study we compared the results of culture and PCR testing of vaginal and rectal samples collected by nursing staff when women were in labor. Patients' charts were also reviewed to obtain relevant information about pregnancy risk factors. Our results demonstrated a sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 90...
September 2010: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/19732424/evaluation-of-a-novel-real-time-pcr-test-based-on-the-ssra-gene-for-the-identification-of-group-b-streptococci-in-vaginal-swabs
#13
Martina Wernecke, Ciara Mullen, Vimla Sharma, John Morrison, Thomas Barry, Majella Maher, Terry Smith
BACKGROUND: Despite the implementation of prevention guidelines, early-onset group B streptococci (GBS) disease remains a cause of neonatal morbidity and mortality worldwide. Strategies to identify women who are at risk of transmitting GBS to their infant and the administration of intrapartum antibiotics have greatly reduced the incidence of neonatal GBS disease. However, there is a requirement for a rapid diagnostic test for GBS that can be carried out in a labour ward setting especially for women whose GBS colonisation status is unknown at the time of delivery...
2009: BMC Infectious Diseases
https://www.readbyqxmd.com/read/19580414/diagnostic-accuracy-of-a-rapid-real-time-polymerase-chain-reaction-assay-for-universal-intrapartum-group-b-streptococcus-screening
#14
COMPARATIVE STUDY
Najoua El Helali, Jean-Claude Nguyen, Aïcha Ly, Yves Giovangrandi, Ludovic Trinquart
BACKGROUND: Intrapartum antibiotic prophylaxis is currently given to mothers who test positive for group B streptococcus (GBS) by antenatal culture-based screening, with a risk-based approach for cases with an unknown GBS status. A rapid real-time polymerase chain reaction (PCR) assay for the detection of GBS became available recently, making intrapartum screening possible. We aimed to assess its diagnostic accuracy and to compare it with antenatal screening. METHODS: We conducted a prospective study in a French hospital...
August 1, 2009: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/18845045/an-evaluation-of-a-rapid-real-time-polymerase-chain-reaction-assay-for-detection-of-group-b-streptococcus-as-part-of-a-neonatal-group-b-streptococcus-prevention-strategy
#15
Deborah Money, Simon Dobson, Lesley Cole, Eda Karacabeyli, Edith Blondel-Hill, Ruth Milner, Eva Thomas
OBJECTIVE: To evaluate the sensitivity, specificity, and feasibility of a rapid real-time polymerase chain reaction (PCR) test for group B streptococcus (GBS) completed during labour, compared with the standard culture test performed at 35 to 37 weeks' gestation. METHODS: Women presenting to the maternity unit for term vaginal delivery had two vaginal/rectal samples collected. One swab was tested using a rapid PCR method (IDI-Strep B, Infectio Diagnostic [IDI] Inc...
September 2008: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/17904971/a-comparison-of-a-new-rapid-real-time-polymerase-chain-reaction-system-to-traditional-culture-in-determining-group-b-streptococcus-colonization
#16
COMPARATIVE STUDY
Michael Gavino, Eileen Wang
OBJECTIVE: The objective of the study was to evaluate a rapid real-time polymerase chain reaction (PCR)-based assay for intrapartum detection of group B streptococcus (GBS). STUDY DESIGN: This prospective, observational study enrolled outpatients after GBS screening at 35-37 weeks' gestation. At admission for delivery, paired rectovaginal swabs were obtained for the GBS GeneXpert (Cepheid, Sunnyvale, CA) assay and standard culture. Using the intrapartum culture as the gold standard, sensitivities, specificities, and predictive values of the rapid assay and the antenatal screen were determined...
October 2007: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/17244804/real-time-pcr-targeting-the-sip-gene-for-detection-of-group-b-streptococcus-colonization-in-pregnant-women-at-delivery
#17
COMPARATIVE STUDY
Hakon Bergseng, Lars Bevanger, Marite Rygg, Kare Bergh
Group B streptococcus (GBS) is an important aetiological agent of serious neonatal infections. A rapid and sensitive method for the detection of GBS colonization in pregnant women at delivery could make intrapartum screening for GBS possible. A real-time PCR method targeting the sip gene of GBS in pregnant women at delivery has been evaluated. The performance of the real-time PCR was compared with optimized GBS culture. Separate vaginal and rectal swabs were collected from women hospitalized at the delivery department at St Olavs Hospital, Trondheim, Norway, from January 15 through May 2005...
February 2007: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/16946205/evaluation-of-polymerase-chain-reaction-for-group-b-streptococcus-detection-using-an-improved-culture-method
#18
COMPARATIVE STUDY
Kristin L Atkins, Robyn M Atkinson, Anthony Shanks, Curtis A Parvin, W Michael Dunne, Gilad Gross
OBJECTIVE: The administration of antibiotic prophylaxis to laboring women who harbor Group B streptococci (GBS) depends on identification of carriers. We sought to evaluate the diagnostic accuracy of real-time polymerase chain reaction (PCR) for detection of GBS using a more stringent culture method. METHODS: Two swabs were used simultaneously to obtain rectovaginal GBS samples from consenting women. One swab was analyzed using a stringent, validated culture technology, which included direct plating onto selective agar and inoculation of a selective broth...
September 2006: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/16585299/rapid-tests-for-group-b-streptococcus-colonization-in-laboring-women-a-systematic-review
#19
REVIEW
Honest Honest, Sushma Sharma, Khalid S Khan
OBJECTIVES: We set out to determine the accuracy and rapidity of various intrapartum group B Streptococcus (GBS) colonization tests. STUDY DESIGN: We performed a systematic review of test-accuracy studies, which were identified without language restriction from Medline and Cochrane databases; bibliographies of known primary and review articles; and contact with authors, experts, and manufacturers. Studies were selected if they tested pregnant women intrapartum for GBS colonization and confirmed by "gold-standard" laboratory cultures...
April 2006: Pediatrics
https://www.readbyqxmd.com/read/15232199/detection-of-group-b-streptococci-gbs-in-vaginal-swabs-using-real-time-pcr-with-taqman-probe-hybridization
#20
K Bergh, A Stoelhaug, K Loeseth, L Bevanger
BACKGROUND & OBJECTIVES: Implementation of a screening based strategy for the prevention of perinatal group B streptococcus (GBS) disease is anticipated to increase the demand of fast laboratory techniques. The aim of the present study was to develop a real-time PCR method for the specific detection of GBS in vaginal swabs. METHODS: Based on partial sequencing of the sip gene, primers and a TaqMan hybridization probe were constructed for a real-time PCR assay. The performance of the assay was tested on 100 consecutive vaginal specimens submitted to the laboratory for culture...
May 2004: Indian Journal of Medical Research
keyword
keyword
45738
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"