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https://www.readbyqxmd.com/read/28533788/grapevine-subtilase-family-update-on-new-sequences-and-nomenclature-proposal
#1
Joana Figueiredo, Marisa Maia, Marta Sousa Silva, Andreia Figueiredo
In grapevine, serine peptidases from the subtilase family were recently associated to Plasmopara viticola resistance. This family in grapevine, first characterized in 2014, was re-analyzed last year and 82 subtilase genes were identified. However, in November of 2016, the National Center for Biotechnology Information database (NCBI) made a new public release of the grapevine genome annotation based on new sequencing data and better prediction algorithms. As a consequence, some gene annotations and lengths changed...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28524029/-predictive-value-of-left-ventricular-diastolic-dysfunction-on-mechanical-ventilation-weaning
#2
Hui Wang, Ming Ma, Desheng Chen, Gang Li, Shupeng Wang, Jingen Xia, Jun Duan
OBJECTIVE: To investigate the predictive value of left ventricular diastolic function on mechanical ventilation weaning in patients with left ventricular ejection fraction (LVEF) > 0.50. METHODS: A retrospective case control study was conducted. Sixty-five patients with LVEF > 0.50 undergoing mechanical ventilation for more than 48 hours admitted to surgery intensive care unit (ICU) of China-Japan Friendship Hospital from June 2014 to December 2016 were enrolled...
May 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28468568/impact-of-low-dose-ketamine-on-the-usage-of-continuous-opioid-infusion-for-the-treatment-of-pain-in-adult-mechanically-ventilated-patients-in-surgical-intensive-care-units
#3
Jessica L Buchheit, Daniel Dante Yeh, Matthias Eikermann, Hsin Lin
BACKGROUND: Ketamine at subanesthetic doses has been shown to provide analgesic effects without causing respiratory depression and may be a viable option in mechanically ventilated patients to assist with extubation. The aim of this study was to evaluate the effects of low-dose ketamine on opioid consumption in mechanically ventilated adult surgical intensive care unit (ICU) patients. METHODS: A retrospective review of mechanically ventilated adult patients receiving low-dose ketamine continuous infusion (1-5 µcg/kg/min) for adjunctive pain control admitted to surgical ICUs was conducted...
January 1, 2017: Journal of Intensive Care Medicine
https://www.readbyqxmd.com/read/28467631/from-structure-to-function-a-family-portrait-of-plant-subtilases
#4
REVIEW
Andreas Schaller, Annick Stintzi, Susana Rivas, Irene Serrano, Nina V Chichkova, Andrey B Vartapetian, Dana Martínez, Juan J Guiamét, Daniela J Sueldo, Renier A L van der Hoorn, Vicente Ramírez, Pablo Vera
I. II. III. IV. V. References SUMMARY: Subtilases (SBTs) are serine peptidases that are found in all three domains of life. As compared with homologs in other Eucarya, plant SBTs are more closely related to archaeal and bacterial SBTs, with which they share many biochemical and structural features. However, in the course of evolution, functional diversification led to the acquisition of novel, plant-specific functions, resulting in the present-day complexity of the plant SBT family. SBTs are much more numerous in plants than in any other organism, and include enzymes involved in general proteolysis as well as highly specific processing proteases...
May 3, 2017: New Phytologist
https://www.readbyqxmd.com/read/28467468/model-based-classification-of-cpt-data-and-automated-lithostratigraphic-mapping-for-high-resolution-characterization-of-a-heterogeneous-sedimentary-aquifer
#5
Bart Rogiers, Dirk Mallants, Okke Batelaan, Matej Gedeon, Marijke Huysmans, Alain Dassargues
Cone penetration testing (CPT) is one of the most efficient and versatile methods currently available for geotechnical, lithostratigraphic and hydrogeological site characterization. Currently available methods for soil behaviour type classification (SBT) of CPT data however have severe limitations, often restricting their application to a local scale. For parameterization of regional groundwater flow or geotechnical models, and delineation of regional hydro- or lithostratigraphy, regional SBT classification would be very useful...
2017: PloS One
https://www.readbyqxmd.com/read/28438880/testicular-dose-during-prophylaxis-of-heterotopic-ossification-with-radiation-therapy
#6
Waleed F Mourad, John K Ma, Satyaseelan Packianathan, Weisi Yan, Sherif G Shaaban, Edward M Marchan, Lamiaa E Abdallah, Rei He, Paul N Mobit, Chunli Claus Yang, Srinivasan Vijayakumar
AIM: A single-institution, retrospective study was performed to investigate potential techniques to minimize radiation exposure to the testicles during heterotopic ossification (HO) prophylaxis. We report the impact of split-beam technique (SBT) and different photon energies on the total dose of radiation received by the testicles during prophylaxis of HO. MATERIALS AND METHODS: Between 2008 and 2010, we identified 64 patients with traumatic acetabular fractures who underwent surgery followed by radiation therapy (RT) without testicular shielding...
May 2017: In Vivo
https://www.readbyqxmd.com/read/28431935/diversity-changes-of-microbial-communities-into-hospital-surface-environments
#7
Rika Yano, Tomoko Shimoda, Reina Watanabe, Yasutoshi Kuroki, Torahiko Okubo, Shinji Nakamura, Junji Matsuo, Sadako Yoshimura, Hiroyuki Yamaguchi
Previous works have demonstrated considerable variability in hospital cleanliness in Japan, suggesting that contamination is driven by factors that are currently poorly controlled. We undertook 16S rRNA sequence analysis to study population structures of hospital environmental microbiomes to see which factor(s) impacted contamination. One hundred forty-four samples were collected from surfaces of three hospitals with distinct sizes ("A": >500 beds, "B": 100-500 beds, "C": <100 beds). Sample locations of two ward types (Surgical and Internal) included patient room bed table (multiple) (4BT), patient overbed table (multiple) (4OT), patient room sink (multiple) (4S), patient room bed table (single) (SBT), patient overbed table (single) (SOT), patient room sink (single) (SS), nurse desk (ND), and nurse wagon (NW)...
April 18, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28429213/effect-of-compound-sbt-828-a-new-indole-derivative-exhibiting-antiaggregant-activity-on-the-prostacyclin-thromboxane-a2-balance
#8
A F Kucheryavenko, A A Spasov, M Tian, K F Suzdalev
We investigated the effect of a new indole derivative Sbt-828 with antiaggregant properties on prostacyclin-generating activity of the vascular wall and thromboxane A2 level in platelets of intact rats. The substance under study did not affect prostacyclin production by the vascular wall and significantly reduced thromboxane A2 level, being superior to the reference drug acetylsalicylic acid by 1.6 times, as seen from reduced malonic dialdehyde level in the thrombin-induced rat platelets.
April 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28428132/novel-properties-of-hippophae-rhamnoides-l-twig-and-leaf-extracts-anti-virulence-action-and-synergy-with-antifungals-studied-in%C3%A2-vitro-on-candida-spp-model
#9
Beata Sadowska, Aleksandra Budzyńska, Anna Stochmal, Jerzy Żuchowski, Barbara Różalska
Original, chemically characterized Sea buckthorn (SBT) twig and leaf extracts were in vitro studied in terms of anti-Candida activity. Minimum inhibitory concentrations (MICs) of the extracts against C. albicans ATCC 10231 ranged: 250 μg/ml (twig), 31.5 μg/ml (leaf), and against C. glabrata G1 (clinical isolate) - 15.6 μg/ml (twig), 3.9 μg/ml (leaf). Next the extracts have been used at their subMIC. Both extracts significantly enhanced activity of fluconazole (FLC) and caspofungin (CAS) against C...
April 18, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28427857/vitrified-warmed-single-embryo-transfers-may-be-associated-with-increased-maternal-complications-compared-with-fresh-single-embryo-transfers
#10
Tal Shavit, Galia Oron, Son Weon-Young, Hananel Holzer, Togas Tulandi
Cryopreservation of embryos allows single-embryo transfer and storage of supernumerary embryos, maximizing cumulative pregnancy rates. The purpose of this retrospective cohort study was to compare pregnancy outcome in singletons born after fresh or vitrified-warmed single blastocyst transfer (SBT). Singleton live births resulting from SBT of fresh or vitrified-warmed embryos were compared. Primary outcomes were perinatal outcomes including small for gestational age (SGA), low birthweight, preterm deliveries (PTD), large for gestational age (LGA) and congenital malformations...
April 6, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28424628/branchial-pathomorphology-of-southern-bluefin-tuna-thunnus-maccoyii-castelnau-1872-infected-by-helminth-and-copepodan-parasites
#11
Mark B Adams, Craig J Hayward, Barbara F Nowak
Three metazoan parasites, a monogenean Hexostoma thynni and two species of copepods Pseudocycnus appendiculatus and Euryphorus brachypterus are known to parasitize the gills of ranched southern bluefin tuna (SBT) and other tuna species. However, there is no detailed information describing the pathological response to infection by these parasites in this species. Wild southern bluefin tuna Thunnus maccoyii (approximately 3 years of age), captured and towed to a grow-out site in the waters immediately south of Port Lincoln, South Australia were subsequently sampled (n = 10) monthly from March until August 2004 during commercial harvest operations...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28422563/effects-of-anterior-knee-displacement-during-squatting-on-patellofemoral-joint-stress
#12
Thomas W Kernozek, Naghmeh Gheidi, Matthew Zellmer, Jordan Hove, Becky L Heinert, Michael R Torry
CONTEXT: Squatting is a common rehabilitation training exercise for Patellofemoral pain syndrome (PFPS). Patellofemoral joint stress (PFJS) during squatting with more anterior knee displacement has not been systematically investigated. OBJECTIVE: The aim was to compare PFJS during squatting using two techniques: Squat while keeping the knees before the toes (SBT) and squat while allowing the knees to go past the toes (SPT). SETTING: University research laboratory...
April 19, 2017: Journal of Sport Rehabilitation
https://www.readbyqxmd.com/read/28406846/the-value-of-simulation-based-training-in-the-path-to-laparoscopic-urological-proficiency
#13
Ben S E P Van Cleynenbreugel, Ali S Gözen, Theodoros Tokas
PURPOSE OF REVIEW: Numerous factors have reduced the training opportunities of surgical residents in the operating room. There is also the question of patient's safety. This gives a need for increased training opportunities outside the operating room. We look if there is an added value in simulation-based training (SBT) in the acquisition of laparoscopic skills. RECENT FINDINGS: Incorporating SBT into the medical students curriculum reduces surgical complication rates in the operating room...
April 12, 2017: Current Opinion in Urology
https://www.readbyqxmd.com/read/28405178/utilizing-bd-max%C3%A2-enteric-bacterial-panel-to-detect-stool-pathogens-from-rectal-swabs
#14
Barbara DeBurger, Sarah Hanna, Eleanor A Powell, Cindi Ventrola, Joel E Mortensen
BACKGROUND: The BD MAX™ Enteric Bacterial Panel (BDM-EBP) is designed and FDA-cleared to detect Salmonella, Shigella, Campylobacter, and Shiga toxin genes stx1/2 from stool samples. However, rectal swabs, which are not FDA-cleared for clinical testing with the BDM-EBP, are common specimens received from pediatric patients for enteric pathogen testing. The purpose of this study was to evaluate the ability of the BDM-EBP to detect stool pathogens from rectal swabs. METHODS: Routine cultures, Shiga toxin testing, and molecular testing with BDM-EBP were performed on 272 sequential rectal swabs collected from August 2015 to December 2015...
2017: BMC Clinical Pathology
https://www.readbyqxmd.com/read/28397235/-allelic-diversity-of-kir2dl4-gene-and-identification-of-five-novel-alleles-among-southern-han-chinese-population
#15
Guobin Zhang, Zhihui Deng
OBJECTIVE: To study the polymorphisms of the KIR2DL4 gene in a southern Han Chinese population. METHODS: Genomic DNA isolated from 306 unrelated individuals was amplified by using KIR2DL4-specific PCR primers. The PCR products were genotyped for the entire coding sequence by sequencing-based typing (SBT). Assignment of allelic profile was accomplished with an Assign 3.5 software. For samples with inconclusive SBT results, the RT-PCR products covering the entire coding sequence of the KIR2DL4 gene were subjected to cloning and haplotype sequencing...
April 10, 2017: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/28396241/molecular-typing-of-legionella-pneumophila-from-air-conditioning-cooling-waters-using-mip-gene-sbt-and-faflp-methods
#16
Xiangli Gong, Juntao Li, Ying Zhang, Shuiping Hou, Pinghua Qu, Zhicong Yang, Shouyi Chen
Legionella spp. are important waterborne pathogens. Molecular typing has become an important method for outbreaks investigations and source tracking of Legionnaires. In a survey program conducted by the Guangzhou Center for Disease Control and Prevention, multiple serotypes Legionella pneumophila (L. pneumophila) were isolated from waters in air-conditioning cooling towers in urban Guangzhou region, China between 2008 and 2011. Three genotyping methods, mip (macrophage infectivity potentiator) genotyping, SBT (sequence-based typing), and FAFLP (fluorescent amplified fragment length polymorphism analysis) were used to type these waterborne L...
April 7, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28386887/a-soil-biotechnology-system-for-wastewater-treatment-technical-hygiene-environmental-lca-and-economic-aspects
#17
Sheetal Jaisingh Kamble, Yogita Chakravarthy, Anju Singh, Caroline Chubilleau, Markus Starkl, Itee Bawa
Soil biotechnology (SBT) is a green engineering approach for wastewater treatment and recycling. Five SBT units located in the Mumbai region were under consideration of which holistic assessment of two SBT plants was carried out considering its technical, environmental and economic aspects and was compared with published research of other three. LCA has been done to evaluate the environmental impacts of construction and operation phase of SBT. Chemical oxygen demand (COD) and biochemical oxygen demand (BOD) removal of more than 90% can be achieved using this technology...
April 6, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28379935/simulation-fellowship-programs-an-international-survey-of-program-directors
#18
Brenda Natal, Demian Szyld, Scott Pasichow, Zia Bismilla, Jonathan Pirie, Adam Cheng
PURPOSE: To report on the evolution of simulation-based training (SBT) by identifying the composition and infrastructure of existing simulation fellowship programs, describing the current training practices, disclosing existing program barriers, and highlighting opportunities for standardization. METHOD: Investigators conducted a cross-sectional survey study among English-speaking simulation fellowship program directors (September 2014-September 2015). They identified fellowships through academic/institutional Web sites, peer-reviewed literature, Web-based search engines, and snowball sampling...
April 4, 2017: Academic Medicine: Journal of the Association of American Medical Colleges
https://www.readbyqxmd.com/read/28371368/a-new-mica-allele-mica-007-07-characterized-by-cloning-and-sequencing
#19
W Y Wang, W Tian, F Wang, F M Zhu, P X Wang, L F Xing
A new MICA allelic variant, MICA*007:07, was identified in an individual of Mongol ethnicity in the Inner Mongolia Autonomous Region, northern China. Following polymerase chain reaction-sequence-based typing (PCR-SBT), this new allele was further confirmed by cloning and sequencing. MICA*007:07 differs from MICA*007:01 by a synonymous mutation from G to A at the 2nd nucleotide position in exon 2. MICA*007:07 was linked to HLA-B*27:05.
March 29, 2017: International Journal of Immunogenetics
https://www.readbyqxmd.com/read/28370062/simultaneous-genotyping-of-human-platelet-alloantigen-1-to-28bw-systems-by-multiplex-polymerase-chain-reaction-sequence-based-typing
#20
X Hong, S Chen, Y Ying, Y Liu, X Xu, J He, F Zhu
BACKGROUND AND OBJECTIVES: Human platelet alloantigen (HPA) genotyping is important for the diagnosis and prevention the alloimmune platelet disorders. In this study, a simultaneous genotyping method for HPA-1 to -28bw systems was established using multiplex PCR-SBT and the frequencies of genotypes and alleles of HPA-1 to -28bw systems in the Zhejiang Han population were analysed. MATERIALS AND METHODS: The specific primers were designed according to the nucleotide sequences of HPA-1 to 28bw systems which are located in ITGB3, GP1BA, ITGA2B, ITGA2, GP1BB and CD109, respectively...
March 31, 2017: Vox Sanguinis
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