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https://www.readbyqxmd.com/read/29676515/characterization-of-a-novel-allelic-variant-in-hla-b-40-lineage-hla-b-40-298-02-by-cloning-and-sequencing
#1
W Tian, F M Zhu, W Y Wang, L X Li, J H Cai
A novel allelic variant in HLA-B*40 lineage, HLA-B*40:298:02, has been identified in an individual of Han ethnicity afflicted with nasopharyngeal carcinoma in Hunan province, southern China. Following polymerase chain reaction-Sanger sequence-based typing (PCR-SBT), this new variant was further confirmed by two distinct strategies of cloning and sequencing. HLA-B*40:298:02 differs from HLA-B*40:298:01 by a single synonymous cytosine substitution at nucleotide position 26 (T→C) in exon 3, which corresponds to codon 99 of the mature HLA-B mRNA molecule...
April 20, 2018: International Journal of Immunogenetics
https://www.readbyqxmd.com/read/29666295/lung-ultrasound-prior-to-spontaneous-breathing-trial-is-not-helpful-in-weaning-the-decision-making-process
#2
Ana Carolina P Antonio, Marli M Knorst, Cassiano Teixeira
BACKGROUND: Lung ultrasound is increasingly becoming a diagnostic tool in the critical care setting. B-pattern on a lung ultrasound is an artifact composed of multiple B-lines and correlates with interstitial edema. A randomized controlled trial concluded that bedside thoracic ultrasound could predict postextubation distress through changes in lung aeration during a weaning procedure; however, it could not screen patients before performance of a spontaneous breathing trial (SBT). METHODS: We conducted a 2-year, prospective, multicenter, observational study in 2 adult medical-surgical ICUs in southern Brazil...
April 17, 2018: Respiratory Care
https://www.readbyqxmd.com/read/29665929/-anti-cd36-mediated-platelet-transfusion-refractoriness-and-related-cases-after-stem-cell-transplantation
#3
Yan Zhou, Li-Lan Li, Zhou-Lin Zhong, Xue-Jun Liu, Jin-Lian Liu, Wei-Dong Shen, Guo-Guang Wu
OBJECTIVE: To analyse the cases of platelet transfusion refractoriness after received HLA-matched unrelated donor hematopoietic stem cell transplantation, to analyze and identify the phenotype and genotype of CD36 in both the patient and stem cell donor, as well as the characteristic of antibody induced platelet transfusion refractoriness, and to analyse the efficacy of matched CD36-deficiency platelets transfusions. METHODS: The CD36 expression on platelet and monocyte was analyzed by flow cytometry (FCM) in both patient and donor...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29643948/central-venous-oxygen-saturation-as-a-predictor-of-a-successful-spontaneous-breathing-trial-from-mechanical-ventilation-a-prospective-nested-case-control-study
#4
Ioannis Georgakas, Afroditi K Boutou, Georgia Pitsiou, Ioannis Kioumis, Milly Bitzani, Kristina Matei, Paraskevi Argyropoulou, Ioannis Stanopoulos
Background: Weaning from mechanical ventilation is a key element in the care of critically ill patients, and Spontaneous Breathing Trial (SBT) is a crucial step in this procedure. This nested case-control study aimed to evaluate whether central oxygen saturation (ScvO2 ) values and their changes could independently predict the SBT outcome among mechanically ventilated patients. Methods: A prospective cohort of patients who were mechanically ventilated for at least 48hours and fulfilled the criteria of readiness to wean constituted the study population...
2018: Open Respiratory Medicine Journal
https://www.readbyqxmd.com/read/29620704/learners-perceptions-during-simulation-based-training-an-interview-study-comparing-remote-versus-locally-facilitated-simulation-based-training
#5
Margrethe Duch Christensen, Doris Oestergaard, Peter Dieckmann, Leonie Watterson
INTRODUCTION: Remotely facilitated simulation-based training (RF-SBT) is less positively appraised than face-to-face, locally facilitated simulation-based training (LF-SBT), despite being considered as an acceptable alternative. This study compared the perceptions of learners after RF-SBT and LF-SBT to explain differences between the two and identify relevant theories that would guide future practice. METHODS: Telephone interviews were conducted with 21 newly graduated doctors and nurses who completed a standardized simulation course delivered in both RF-SBT and LF-SBT formats...
April 3, 2018: Simulation in Healthcare: Journal of the Society for Simulation in Healthcare
https://www.readbyqxmd.com/read/29602747/the-predictive-ability-of-the-start-back-tool-was-limited-in-people-with-chronic-low-back-pain-a-prospective-cohort-study
#6
Michelle Kendell, Darren Beales, Peter O'Sullivan, Martin Rabey, Jonathan Hill, Anne Smith
QUESTIONS: In people with chronic non-specific low back pain (LBP), what is the predictive and discriminative validity of the STarT Back Tool (SBT) for pain intensity, self-reported LBP-related disability, and global self-perceived change at 1-year follow-up? What is the profile of the SBT risk subgroups with respect to demographic variables, pain intensity, self-reported LBP-related disability, and psychological measures? DESIGN: Prospective cohort study. PARTICIPANTS: A total of 290 adults with dominant axial LBP of≥3months' duration recruited from the general community, and private physiotherapy, psychology, and pain-management clinics in Western Australia...
March 27, 2018: Journal of Physiotherapy
https://www.readbyqxmd.com/read/29564755/choice-and-outcomes-of-rate-control-versus-rhythm-control-in-elderly-patients-with-atrial-fibrillation-a-report-from-the-reposi-study
#7
Francesco Paciullo, Marco Proietti, Vanessa Bianconi, Alessandro Nobili, Matteo Pirro, Pier Mannuccio Mannucci, Gregory Y H Lip, Graziana Lupattelli
BACKGROUND: Among rate-control or rhythm-control strategies, there is conflicting evidence as to which is the best management approach for non-valvular atrial fibrillation (AF) in elderly patients. DESIGN: We performed an ancillary analysis from the 'Registro Politerapie SIMI' study, enrolling elderly inpatients from internal medicine and geriatric wards. METHODS: We considered patients enrolled from 2008 to 2014 with an AF diagnosis at admission, treated with a rate-control-only or rhythm-control-only strategy...
March 21, 2018: Drugs & Aging
https://www.readbyqxmd.com/read/29563252/advancing-weaning-science-shorter-or-longer-duration-of-sbt-illuminating-the-road-ahead
#8
EDITORIAL
Rahul S Nanchal, Jonathon D Truwit
No abstract text is available yet for this article.
April 2018: Respiratory Care
https://www.readbyqxmd.com/read/29556805/serial-diaphragm-ultrasonography-to-predict-successful-discontinuation-of-mechanical-ventilation
#9
Atul Palkar, Paul Mayo, Karan Singh, Seth Koenig, Mangala Narasimhan, Anup Singh, Rivkah Darabaner, Harly Greenberg, Eric Gottesman
INTRODUCTION: Diaphragm excursion and contraction velocity measured using ultrasonography have been used to assess diaphragm function. We aimed to evaluate the performance of diaphragm ultrasonography during weaning from mechanical ventilation (MV). METHODS: Diaphragm ultrasonography was performed on 73 mechanically ventilated patients who were being considered for extubation on three separate occasions: (1) on assist control mode (A/C) during consistent patient triggered ventilation, (2) following 30 min during a spontaneous breathing trial (SBT), (3) 4-24 h following extubation...
March 19, 2018: Lung
https://www.readbyqxmd.com/read/29549205/diagnostic-accuracy-of-the-ottawa-3dy-and-short-blessed-test-to-detect-cognitive-dysfunction-in-geriatric-patients-presenting-to-the-emergency-department
#10
David Barbic, Brian Kim, Qadeem Salehmohamed, Kate Kemplin, Christopher R Carpenter, Skye Pamela Barbic
OBJECTIVES: Cognitive dysfunction (CD) is a common finding in geriatric patients presenting to the emergency department (ED). Our primary objective was to determine the diagnostic accuracy of the Ottawa 3DY (O3DY) and Short Blessed Test (SBT) as screening tools for the detection of CD in the ED. Our secondary objective was to estimate the inter-rater reliability of these instruments. METHODS: We conducted a prospective cross-sectional comparative study at an inner-city academic medical centre (annual ED visit census 86 000)...
March 16, 2018: BMJ Open
https://www.readbyqxmd.com/read/29549055/low-genomic-diversity-of-legionella-pneumophila-within-clinical-specimens
#11
Sophia David, Massimo Mentasti, Julian Parkhill, Victoria J Chalker
OBJECTIVES: Legionella pneumophila is the leading cause of Legionnaires' disease, a severe form of pneumonia acquired from environmental sources. Investigations of both sporadic cases and outbreaks mostly rely on analysis of a single to a few colony pick(s) isolated from each patient. However, due to the lack of data describing diversity within single patients, the optimal number of picks is unknown. Here, we investigated diversity within individual patients using sequence based typing (SBT) and whole genome sequencing (WGS)...
March 13, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29524068/gut-microbiota-and-its-implications-in-small-bowel-transplantation
#12
REVIEW
Chenyang Wang, Qiurong Li, Jieshou Li
The gut microbiota is mainly composed of a diverse population of commensal bacterial species and plays a pivotal role in the maintenance of intestinal homeostasis, immune modulation and metabolism. The influence of the gut microbiota on solid organ transplantation has recently been recognized. In fact, several studies indicated that acute and chronic allograft rejection in small bowel transplantation (SBT) is closely associated with the alterations in microbial patterns in the gut. In this review, we focused on the recent findings regarding alterations in the microbiota following SBTand the potential roles of these alterations in the development of acute and chronic allograft rejection...
March 9, 2018: Frontiers of Medicine
https://www.readbyqxmd.com/read/29520477/biodistribution-and-pharmacokinetic-evaluations-of-a-novel-taxoid-dha-sbt-1214-in-an-oil-in-water-nanoemulsion-formulation-in-na%C3%A3-ve-and-tumor-bearing-mice
#13
Gulzar Ahmad, Florence Gattacecca, Rana El Sadda, Galina Botchkina, Iwao Ojima, James Egan, Mansoor Amiji
PURPOSE: The main purpose of this study was to formulate an oil-in-water nanoemulsion of a next generation taxoid DHA-SBT-1214 and evaluate its biodistribution and pharmacokinetics. METHODS: DHA-SBT-1214 was encapsulated in a fish oil containing nanoemulsion using a high pressure homogenization method. Following morphological characterization of the nanoemulsions, qualitative and quantitative biodistribution was evaluated in naïve and cancer stem cell-enriched PPT-2 human prostate tumor bearing mice...
March 8, 2018: Pharmaceutical Research
https://www.readbyqxmd.com/read/29509509/international-practice-variation-in-weaning-critically-ill-adults-from-invasive-mechanical-ventilation
#14
Karen E A Burns, Stavroula Raptis, Rosane Nisenbaum, Leena Rizvi, Andrew Jones, Jyoti Bakshi, Wylie Tan, Aleksander Meret, Deborah J Cook, Francois Lellouche, Scott K Epstein, David Gattas, Farhad N Kapadia, Jesús Villar, Laurent Brochard, Martin R Lessard, Maureen O Meade
RATIONALE: Randomized trials and meta-analyses have informed several aspects of weaning. Results are rarely replicated in practice, as evidence is applied in intensive care units that differ from the settings in which it was generated. OBJECTIVES: We aimed to: 1) describe weaning practice variation (identifying weaning candidates, conducting spontaneous breathing trials, using ventilator modes, and other aspects of care during weaning); 2) characterize regional differences in weaning practices; and 3) identify factors associated with practice variation...
April 2018: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/29503348/trematode-infections-in-farm-animals-and-their-vector-snails-in-saint-martin-s-island-the-southeastern-offshore-area-of-bangladesh-in-the-bay-of-bengal
#15
M Golam Yasin, Md Abdul Alim, Syed Ali Ahasan, Md Nuruzzaman Munsi, Emdadul Haque Chowdhury, Takeshi Hatta, Naotoshi Tsuji, Md Motahar Hussain Mondal
The prevalence of snail-borne trematode (SBT) infections in farm animals on the offshore Saint (St.) Martin's Island of Bangladesh were 68.9% for cattle, 76.7% for buffaloes, 56.3% for goats, respectively. Examination of fecal samples showed that paramphistomes infection was the most common at 50.5% followed by schistosomes at 23.7% and Fasciola at 2.3%. Fasciola infection was found in cattle (1.9%) and buffaloes (16.7%) but not in goats. Schistosome infection in cattle, buffaloes and goats were 31.1%, 6.7% and 17...
March 5, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29489714/effects-of-combined-balance-and-plyometric-training-on-athletic-performance-in-female-basketball-players
#16
Ichrak Bouteraa, Yassine Negra, Roy J Shephard, Mohamed Souhaiel Chelly
The purpose of this study was to examine the effect of 8 weeks combined balance and plyometric training on the physical fitness of female adolescent basketball players. Twenty six healthy regional-level players were randomly assigned to either an experimental group (E; n = 16, age = 16.4 ± 0.5) or a control group (C; n = 10, age = 16.5 ± 0.5). C maintained their normal basketball training schedule, whereas for 8 weeks E replaced a part of their standard regimen by biweekly combined training sessions. Testing before and after training included the Squat Jump (SJ), Countermovement Jump (CMJ), Drop Jump (DJ), 5, 10 and 20-m sprints, Stork Balance Test (SBT), Y-Balance Test (YBT) and Modified Illinois Change of Direction Test (MICODT)...
February 27, 2018: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29489407/ct-features-of-ovarian-tumors-defining-key-differences-between-serous-borderline-tumors-and-low-grade-serous-carcinomas
#17
Stephanie Nougaret, Yulia Lakhman, Nicolas Molinari, Diana Feier, Chiara Scelzo, Hebert A Vargas, Ramon E Sosa, Hedvig Hricak, Robert A Soslow, Rachel N Grisham, Evis Sala
OBJECTIVE: The objective of our study was to investigate whether the CT features of serous borderline tumors (SBTs) differ from those of low-grade serous carcinomas (LGSCs) and to evaluate if mutation status is associated with distinct CT phenotypes. MATERIALS AND METHODS: This retrospective study included 59 women, 37 with SBT and 22 with LGSC, who underwent CT before primary surgical resection. Thirty of 59 patients were genetically profiled. Two radiologists (readers 1 and 2) independently and retrospectively reviewed CT examinations for qualitative features and quantified total tumor volumes (TTVs), solid tumor volumes (STVs), and solid proportion of ovarian masses...
February 28, 2018: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/29485959/a-case-report-of-penicillin-tolerant-streptococcus-mitis-endocarditis-chang-hua-chen-md-msc-phd
#18
Chang-Hua Chen
There is no clear relationship between the serum inhibition test and clinical outcome for Streptococcus mitis (S. mitis) endocarditis. We report an 84-year-old male with endocarditis caused by penicillin-tolerant S. mitis. The results for the serum inhibitory test (SIT) and serum bactericidal test (SBT) showed a trough level of SIT = 1:256 and SBT = 1:4 and a peak level of SIT ≥ 1:1024 and SBT = 1:16. In addition, the SIT/SBT ratio was 64 at peak level and more than 64 at trough level, which is compatible with penicillin-tolerant S...
January 16, 2018: Heart Surgery Forum
https://www.readbyqxmd.com/read/29479163/effect-of-dietary-supplementation-of-sea-buckthorn-and-giloe-leaf-meal-on-the-body-weight-gain-feed-conversion-ratio-biochemical-attributes-and-meat-composition-of-turkey-poults
#19
Aditya Sharma, Pankaj Kumar Shukla, Amitav Bhattacharyya, Upendra Kumar, Debashis Roy, Brijesh Yadav, Atul Prakash
Aim: In the recent past, few studies have been carried out about sea buckthorn (SBT) and giloe in chicken as a part of the quest for suitable alternatives to antibiotics. However, studies in turkeys are lacking. Hence, the present study was conducted to evaluate the effects of SBT and giloe leaf meal by dietary feed supplementation in turkey poults. Materials and Methods: A total of 1-day-old turkey poults (n=84) of small white variety were distributed into four dietary treatments having three replicates each with seven birds...
January 2018: Veterinary World
https://www.readbyqxmd.com/read/29469357/-correlation-between-hla-db1-genes-and-susceptibility-to-echinococcosis-in-tibetan-population-in-tibetan-plateau
#20
Gao Shu-Feng, Han Xiu-Min, Zhang Xue-Fei, Wang Yong-Shun, Wang Wei, Guo Ya-Min, Li Yong-Shou
OBJECTIVE: To determine the susceptibility genes and resistance genes in HLA-DRB1 alleles in Tibetan patients with cystic and alveolar hydatid diseases, so as to provide the references for the research of the genetic characteristics and infection mechanism of Tibetan hydatid diseases. METHODS: The case control method was applied. The Tibetan patients with cystic and alveolar hydatid diseases (63 and 73 cases respectively) in Yushu and Guoluo Tibetan Autonomous Prefecture, and unrelated healthy people (60 cases) in this area were selected as the study subjects...
October 23, 2017: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
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