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https://www.readbyqxmd.com/read/28315853/effects-of-extracts-and-compounds-from-tricholoma-populinum-lange-on-degranulation-and-il-2-il-8-secretion-of-immune-cells
#1
Simon Merdivan, Kristina Jenett-Siems, Karsten Siems, Timo H J Niedermeyer, Nadin Schultze, Carmen Steinborn, Carsten Gründemann, Ulrike Lindequist
Tricholoma populinum Lange is an edible basidiomycete from the family Tricholomataceae. Extracts, fractions, and different metabolites isolated from the fruiting bodies of this mushroom were tested for degranulation-inhibiting activities on RBL-2H3 cells (rat basophils). Dichloromethane extracts decreased degranulation significantly, as did a fraction after column chromatography. In addition, the extract decreased the IL-2 release from Jurkat T cells and the release of IL-8 from HMC-1 human mast cells. The results show the significant effects of extracts of T...
March 20, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28299505/changes-in-membrane-lipids-drive-increased-endocytosis-following-fas-ligation
#2
Mauro Degli Esposti, Paola Matarrese, Antonella Tinari, Agostina Longo, Serena Recalchi, Roya Khosravi-Far, Walter Malorni, Roberta Misasi, Tina Garofalo, Maurizio Sorice
Once activated, some surface receptors promote membrane movements that open new portals of endocytosis, in part to facilitate the internalization of their activated complexes. The prototypic death receptor Fas (CD95/Apo1) promotes a wave of enhanced endocytosis that induces a transient intermixing of endosomes with mitochondria in cells that require mitochondria to amplify death signaling. This initiates a global alteration in membrane traffic that originates from changes in key membrane lipids occurring in the endoplasmic reticulum (ER)...
March 15, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28293545/helicobacter-pylori-secreted-protein-hp1286-triggers-apoptosis-in-macrophages-via-tnf-independent-and-erk-mapk-dependent-pathways
#3
Raquel Tavares, Sushil Kumar Pathak
Macrophages constitute a powerful line of defense against H. pylori. The final disease outcome is highly dependent on the bacterial ability to modulate the effector functions of activated macrophages. Here, we report that H. pylori secreted protein HP1286 is a novel regulator of macrophage responses. Differential expression and release of HP1286 homologues were observed among H. pylori strains. Recombinant purified HP1286 (rHP1286) had the ability to bind to primary human monocyte-derived macrophages (MDM) and macrophage cell lines...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28289074/t-cell-costimulation-by-cd6-is-dependent-on-bivalent-binding-of-a-gads-slp-76-complex
#4
Johannes Breuning, Marion H Brown
The cell surface receptor, CD6 regulates T cell activation in both an activating and inhibitory manner. The adaptor protein SLP-76 is recruited to the CD6 cytoplasmic phosphorylated Y662 residue during T cell activation, providing an activating signal to T cells. In this study, a biochemical approach identified the SH2 domain-containing adaptor protein GADS as the dominant interaction partner for the CD6 cytoplasmic Y629 residue. Functional experiments in human Jurkat and primary T cells showed that mutation of both Y629F and Y662F abolished costimulation by CD6...
March 13, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/28276767/six-new-c-21-steroidal-glycosides-from-dregea-sinensis-hemsl
#5
Xiu-Jie Liu, Yu Shi, Shao-Hua Jia, Yu-Lin Deng, Fang Lv, Rong-Ji Dai
Six new C-21 steroidal glycosides (1-6) were separated from the root of Dregea sinensis Hemsl. and their structures were elucidated using extensive nuclear magnetic resonance, mass spectrometry, and infrared spectral analyses. Isolated compounds were evaluated for antitumor activity, which showed that compound 3 had moderate activity in Jurkat cells (IC50 19.54 ± 0.91 μM), and compounds 1-4 had significant effects against IL-2R and TNFR2 (IC50 1.518 ± 0.06 μM to 5.9 ± 0.07 μM).
February 13, 2017: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/28275049/the-antineoplastic-activity-of-bardoxolone-methyl-and-a-related-triterpenoid-in-leukemia-cells-is-due-to-reactive-oxygen-species-dependent-suppression-of-cmyc
#6
Unho Jin, Yating Cheng, Beiyan Zhou, Stephen Safe
Structurally-related pentacyclic triterpenoids methyl 2-cyano-3,12-dioxoolean-1,9-dien-28-oate [Bardoxolone-methyl (Bar-Me)] and methyl 2-trifluoromethyl-3,11-dioxoolean-1,12-dien-30-oate (CF3DODA-Me) contain 2-cyano-1-en-3-one and 2-trifluoromethyl-1-en-3-one moieties, respectively, in their A rings and differ in the position of their en-one structures in ring C. Only Bar-Me forms a Michael addition adduct with glutathione and inhibits IKKβ phosphorylation and these differences may be due to steric hinderance by the 11-keto group in CF3DODA-Me which prevents Michael addition by the conjugated en-one in the A-ring...
March 8, 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/28274837/a-novel-method-for-detection-of-ifn-lambda-3-binding-to-cells-for-quantifying-ifn-lambda-receptor-expression
#7
Deanna M Santer, Gillian E S Minty, Adil Mohamed, Lesley Baldwin, Rakesh Bhat, Michael Joyce, Adrian Egli, D Lorne J Tyrrell, Michael Houghton
Type III interferons (IFN-lambdas) are important antiviral cytokines that also modulate immune responses acting through a unique IFN-λR1/IL-10R2 heterodimeric receptor. Conflicting data has been reported for which cells express the IFN-λR1 subunit and directly respond to IFN-λs. In this study we developed a novel method to measure IFN-λ3 binding to IFN-λR1/IL-10R2 on the surface of cells and relate this to a functional readout of interferon stimulated gene (ISG) activity in various cell lines. We show that Huh7...
March 5, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28271957/cobalt-ii-complex-with-novel-unsymmetrical-tetradentate-schiff-base-on-ligand-in-vitro-cytotoxicity-studies-of-complex-interaction-with-dna-protein-molecular-docking-studies-and-antibacterial-activity
#8
Zahra Shokohi-Pour, Hossein Chiniforoshan, Mohammad R Sabzalian, Seyed-Alireza Esmaeili, Amir Abbas Momtazi-Borojeni
[C20H17N3O2] and cobalt (II) complex [Co(L(2))(MeOH)2].ClO4, (L(2) = 4-((E)-1-((2-(((E)-pyridin-2-ylmethylene) amino) phenyl) imino) ethyl) benzene-1, 3-diol) novel Schiff base has been synthesiszed and chracterized by Fourier transform infrared, UV-vis, (1)H-NMR spectroscopy, and elemental analysis techniques. The interaction of Co(II) complex with DNA and BSA was investigated by electronic absorption spectroscopy, fluorescence spectroscopy, circular dichroism, and thermal denaturation studies. Our experiments indicate that this complex could strongly bind to CT-DNA via minor groove mechanism...
March 8, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28266526/elf-mf-exposure-affects-the-robustness-of-epigenetic-programming-during-granulopoiesis
#9
Melissa Manser, Mohamad R Abdul Sater, Christoph D Schmid, Faiza Noreen, Manuel Murbach, Niels Kuster, David Schuermann, Primo Schär
Extremely-low-frequency magnetic fields (ELF-MF) have been classified as "possibly carcinogenic" to humans on the grounds of an epidemiological association of ELF-MF exposure with an increased risk of childhood leukaemia. Yet, underlying mechanisms have remained obscure. Genome instability seems an unlikely reason as the energy transmitted by ELF-MF is too low to damage DNA and induce cancer-promoting mutations. ELF-MF, however, may perturb the epigenetic code of genomes, which is well-known to be sensitive to environmental conditions and generally deranged in cancers, including leukaemia...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28260095/inhibin-%C3%AE-gene-mutations-and-mrna-levels-in-human-lymphoid-and-myeloid-leukemia-cells
#10
Young Il Kim, Seung-Won Park, Hye Shin Kwon, Hyung-Seok Yang, Sun Young Cho, Young Jin Kim, Hee Joo Lee
The inhibin-α gene was identified as a tumor suppressor gene in the gonads and adrenal glands by functional studies using knockout mice. Methylation of CpG sites within the regulatory regions of tumor suppressor gene is frequently associated with their transcriptional silencing. We investigated epigenetic modifications, changes in loss of heterozygosity (LOH), and mutation of the inhibin-α gene, and regulation of transcriptional expression in response to inhibitors of DNA methylation (5-aza-2'-deoxycytidine, 5-AzaC) in human lymphoid (Jurkat, Molt-4, Raji, and IM-9) and myeloid (HL-60, Kasumi-1, and K562) leukemia cells...
March 2, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28259946/%C3%AE-%C3%AE-tcr-immunoglobulin-constant-region-domain-exchange-in-human-%C3%AE-%C3%AE-tcrs-improves-tcr-pairing-without-altering-tcr-gene-modified-t-cell-function
#11
Changli Tao, Hongwei Shao, Wenfeng Zhang, Huaben Bo, Fenglin Wu, Han Shen, Shulin Huang
The adoptive genetic transfer of T cell receptors (TCRs) has been shown to be overall feasible and offer clinical potential as a treatment for different types of cancer. However, this promising clinical approach is limited by the serious potential consequence that exogenous TCR mispairing with endogenous TCR chains may lead to the risk of self-reactivity. In the present study, domain‑exchange and three‑dimensional modeling strategies were used to create a set of chimeric TCR variants, which were used to exchange the partial or complete constant region of αβTCR with corresponding γδTCR domains...
February 15, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28251953/-suppression-of-telomerase-activity-leukemic-cells-by-mutant-forms-of-rhodospirillum-rubrum-l-asparaginase
#12
M V Pokrovskaya, D D Zhdanov, M A Eldarov, S S Aleksandrova, A V Veselovskiy, V S Pokrovskiy, D V Grishin, Ju A Gladilina, N N Sokolov
The active and stable mutant forms of short chain cytoplasmic L-asparaginase type I of Rhodospirillum rubrum (RrA): RrA+N17, D60K, F61L, RrA+N17, A64V, E67K, RrA+N17, E149R, V150P, RrAE149R, V150P and RrAE149R, V150P, F151T were obtained by the method of site-directed mutagenesis. It is established that variants RrA-N17, E149R, V150P, F151T and RrАE149R, V150P are capable to reduce an expression hTERT subunit of telomerase and, hence, activity of telomeres in Jurkat cells, but not in cellular lysates. During too time, L-asparaginases of Escherichia coli, Erwinia carotovora and Wolinella succinogenes, mutant forms RrА+N17, D60K, F61L and RrА+N17, A64V, E67K do not suppress of telomerase activity...
January 2017: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/28251512/preservation-of-anticancer-and-immunosuppressive-properties-of-rapamycin-achieved-through-controlled-releasing-particles
#13
Yan Liang Fan, Han Wei Hou, Hui Min Tay, Wei Mei Guo, Per-Olof Berggren, Say Chye Joachim Loo
Rapamycin is commonly used in chemotherapy and posttransplantation rejection suppression, where sustained release is preferred. Conventionally, rapamycin has to be administered in excess due to its poor solubility, and this often leads to cytotoxicity and undesirable side effects. In addition, rapamycin has been shown to be hydrolytically unstable, losing its bioactivity within a few hours. The use of drug delivery systems is hypothesized to preserve the bioactivity of rapamycin, while providing controlled release of this otherwise potent drug...
March 1, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28247308/differential-maturation-of-mir-17%C3%A2-%C3%A2-92-cluster-members-in-human-cancer-cell-lines
#14
Mozhgan Abasi, Fatemeh Kohram, Parviz Fallah, Arash Arashkia, Masoud Soleimani, Nosratollah Zarghami, Hossein Ghanbarian
While some microRNAs are transcribed from a specific promoter, at least one third of human miRNA genes are clustered, wherein multiple miRNA genes are generated from a single primary transcript such as miR-17 ~ 92 cluster. Although six members of the cluster are generated from a single transcript, the mature level of each member may be diverse in various cell types. Here, we attempt to monitor the mature level of miR-17, miR-92a, and miR-20a from miR-17 ~ 92 cluster in blood (HL60 (human promyelocytic leukemia cells) and Jurkat) and breast (MDA-MB-231 and MCF-7) cancer cell lines...
February 28, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28247205/the-mechanism-of-jurkat-cells-apoptosis-induced-by-aggregatibacter-actinomycetemcomitans-cytolethal-distending-toxin
#15
Hui-Ping Chen, Lu Li, Xu Chen, Mi-Fang Yang, Yu Ye, Xiao-Qian Wang, Yan Xu
Cytolethal distending toxin (CDT) which is produced by Aggregatibacter actinomycetemcomitans causes apoptosis in lymphocytes. But the specific mechanism is not clear. The aim of our research was to investigate the effect and mechanism during this process. The wild-type CdtA, CdtB, CdtC (CdtA(W), CdtB(W), CdtC(W)) and mutant CdtB (CdtB(M)) were expressed and purified respectively and the purity of each subunit was examined by BandScan software. And the type I deoxyribonuclease and PI-3,4,5-triphosphate (PI-3,4,5-P3, PIP3) phosphatase activity were detected by DNA agarose gel electrophoresis and enzyme-linked immunosorbent assay respectively...
February 28, 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/28246360/multiple-histone-lysine-methyltransferases-are-required-for-the-establishment-and-maintenance-of-hiv-1-latency
#16
Kien Nguyen, Biswajit Das, Curtis Dobrowolski, Jonathan Karn
We showed previously that the histone lysine methyltransferase (HKMT) H3K27me3 (EZH2) is the catalytic subunit of Polycomb repressive complex 2 (PRC2) and is required for the maintenance of HIV-1 latency in Jurkat T cells. Here we show, by using chromatin immunoprecipitation experiments, that both PRC2 and euchromatic histone-lysine N-methyltransferase 2 (EHMT2), the G9a H3K9me2-3 methyltransferase, are highly enriched at the proviral 5' long terminal repeat (LTR) and rapidly displaced upon proviral reactivation...
February 28, 2017: MBio
https://www.readbyqxmd.com/read/28239798/effect-of-fibroblast-like-cells-of-mesenchymal-origin-of-cytotoxic-activity-of-lymphocytes-against-nk-sensitive-target-cells
#17
A Yu Lupatov, Ya S Kim, O A Bystrykh, I V Vakhrushev, S V Pavlovich, K N Yarygin, G T Sukhikh
We studied immunosuppressive properties of skin fibroblasts and mesenchymal stromal cells against NK cells. In vitro experiments showed that mesenchymal stromal cells isolated from human umbilical cord and human skin fibroblasts can considerably attenuate cytotoxic activity of NK cells against Jurkat cells sensitive to NK-mediated lysis. NK cells cultured in lymphocyte population exhibited higher cytotoxic activity than isolated NK cells. Mesenchymal stromal cells or fibroblasts added 1:1 to lymphocyte culture almost completely suppressed NK cell cytotoxicity...
February 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28235702/design-synthesis-biological-evaluation-and-x-ray-structural-studies-of-potent-human-dihydroorotate-dehydrogenase-inhibitors-based-on-hydroxylated-azole-scaffolds
#18
Stefano Sainas, Agnese C Pippione, Marta Giorgis, Elisa Lupino, Parveen Goyal, Cristina Ramondetti, Barbara Buccinnà, Marco Piccinini, Rodolpho C Braga, Carolina H Andrade, Mikael Andersson, Ann-Christin Moritzer, Rosmarie Friemann, Stefano Mensa, Salam Al-Kadaraghi, Donatella Boschi, Marco L Lolli
A new generation of potent hDHODH inhibitors designed by a scaffold-hopping replacement of the quinolinecarboxylate moiety of brequinar, one of the most potent known hDHODH inhibitors, is presented here. Their general structure is characterized by a biphenyl moiety joined through an amide bridge with an acidic hydroxyazole scaffold (hydroxylated thiadiazole, pyrazole and triazole). Molecular modelling suggested that these structures should adopt a brequinar-like binding mode involving interactions with subsites 1, 2 and 4 of the hDHODH binding site...
March 31, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28235572/role-of-akt-pkb-and-pfkfb-isoenzymes-in-the-control-of-glycolysis-cell-proliferation-and-protein-synthesis-in-mitogen-stimulated-thymocytes
#19
Amina Houddane, Laurent Bultot, Laura Novellasdemunt, Manuel Johanns, Marie-Agnès Gueuning, Didier Vertommen, Pierre G Coulie, Ramon Bartrons, Louis Hue, Mark H Rider
Proliferating cells depend on glycolysis mainly to supply precursors for macromolecular synthesis. Fructose 2,6-bisphosphate (Fru-2,6-P2) is the most potent positive allosteric effector of 6-phosphofructo-1-kinase (PFK-1), and hence of glycolysis. Mitogen stimulation of rat thymocytes with concanavalin A (ConA) led to time-dependent increases in lactate accumulation (6-fold), Fru-2,6-P2 content (4-fold), 6-phosphofructo-2-kinase (PFK-2)/fructose-2,6-bisphosphatase isoenzyme 3 and 4 (PFKFB3 and PFKFB4) protein levels (~2-fold and ~15-fold, respectively) and rates of cell proliferation (~40-fold) and protein synthesis (10-fold) after 68h of incubation compared with resting cells...
February 22, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28235359/cytotoxic-effects-of-dimorfolido-n-trichloroacetylphosphorylamide-and-dimorfolido-n-benzoylphosphorylamide-in-combination-with-c60-fullerene-on-leukemic-cells-and-docking-study-of-their-interaction-with-dna
#20
S Prylutska, I Grynyuk, A Grebinyk, V Hurmach, Iu Shatrava, T Sliva, V Amirkhanov, Yu Prylutskyy, O Matyshevska, M Slobodyanik, M Frohme, U Ritter
Dimorfolido-N-trichloroacetylphosphorylamide (HL1) and dimorfolido-N-benzoylphosphorylamide (HL2) as representatives of carbacylamidophosphates were synthesized and identified by the methods of IR, (1)H, and (31)P NMR spectroscopy. In vitro HL1 and HL2 at 1 mM concentration caused cell specific and time-dependent decrease of leukemic cell viability. Compounds caused the similar gradual decrease of Jurkat cells viability at 72 h (by 35%). HL1 had earlier and more profound toxic effect as compared to HL2 regardless on leukemic cell line...
December 2017: Nanoscale Research Letters
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