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https://www.readbyqxmd.com/read/28728406/early-weaning-of-calves-after-different-dietary-regimens-affects-later-rumen-development-growth-and-carcass-traits-in-hanwoo-cattle
#1
Kondreddy Eswar Reddy, JinYoung Jeong, Youl-Chang Baek, Young Kyun Oh, Minseok Kim, Kyung Min So, Min Ji Kim, Dong Woon Kim, Sung Kwon Park, Hyun-Jeong Lee
Objective: The main objective of this study was to determine the effect of different diets for early-weaned (EW) calves on rumen development, and how this affects fat deposition in the longissimus dorsi of adult Korean Hanwoo beef cattle. Methods: Three EW groups were established (each n = 12) in which two- week-old Hanwoo calves were fed for ten weeks with milk replacer + concentrate (T1), milk replacer + concentrate + roughage (T2), or milk replacer + concentrate + 30% starch (T3); a control group (n = 12) was weaned as normal...
July 6, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728400/studies-on-meat-color-myoglobin-content-enzyme-activities-and-genes-associated-with-oxidative-potential-of-pigs-slaughtered-at-different-growth-stages
#2
Qin Ping Yu, Ding Yuan Feng, Juan Xiao, Fan Wu, Xiao Jun He, Min Hao Xia, Tao Dong, Yi Hua Liu, Hui Ze Tan, Shi Geng Zou, Tao Zheng, Xian Hua Ou, Jian Jun Zuo
Objective: This experiment was conducted to investigate meat color, myoglobin (Mb) content, enzyme activities, and expression of genes associated with oxidative potential of pigs slaughtered at different growth stages. Methods: Sixty 4-week-old Duroc × Landrace × Yorkshire pigs were assigned to 6 replicate groups, each containing 10 pigs. One pig from each group was sacrificed at day 35, 63, 98, and 161 to isolate longissimus dorsi and triceps muscles. Results: Meat color scores were higher in pigs at 35 d than those at 63 d and 98 d (P < 0...
May 14, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728397/effects-of-dietary-valine-lysine-ratio-on-the-performance-amino-acid-composition-of-tissues-and-mrna-expression-of-genes-involved-in-branched-chain-amino-acid-metabolism-of-weaned-piglets
#3
Yetong Xu, Xiaokang Ma, Chunli Wang, Mingfeng Yuan, Xiangshu Piao
Objective: The goal of this study was to investigate the effects of dietary standard ileal digestible (SID) valine:lysine ratios on performance, intestinal morphology, amino acids of liver and muscle, plasma indices and mRNA expression of branched-chain amino acid (BCAA) metabolism enzymes. Methods: A total of 144 crossbred pigs (Duroc × Landrace × Large White) weaned at 28 ± 4 days of age (8.79 ± 0.02 kg body weight) were randomly allotted to 1 of 4 diets formulated to provide SID valine: lysine ratios of 50, 60, 70 or 80%...
May 14, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728396/characterization-and-functional-inferences-of-a-genome-wide-dna-methylation-profile-in-the-loin-longissimus-dorsi-muscle-of-swine
#4
Woonsu Kim, Hyesun Park, Kang-Seok Seo, Seongwon Seo
Objective: DNA methylation plays a major role in regulating the expression of genes related to traits of economic interest (e.g., weight gain) in livestock animals. This study characterized and investigated the functional inferences of genome-wide DNA methylome in the loin (longissimus dorsi) muscle (LDM) of swine. Methods: A total of 8.99 Gb methylated DNA immunoprecipitation sequence data were obtained from LDM samples of eight Duroc pigs (four pairs of littermates)...
May 14, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728385/effects-of-dietary-energy-sources-on-early-postmortem-muscle-metabolism-of-finishing-pigs
#5
Yanjiao Li, Changning Yu, Jiaolong Li, Lin Zhang, Feng Gao, Guanghong Zhou
Objective: This study investigated the effects of different dietary energy sources on early postmortem muscle metabolism of finishing pigs. Methods: Seventy-two barrow (Duroc × Landrace × Yorkshire, DLY) pigs (65.0 ± 2.0 kg) were allotted to three iso-energetic and iso-nitrogenous diets: A (44.1% starch, 5.9% crude fat and 12.6% neutral detergent fibre (NDF)), B (37.6% starch, 9.5% crude fat and 15.4% NDF) or C (30.9% starch, 14.3% crude fat and 17.8% NDF). After the duration of 28-day feeding experiment, 24 pigs (eight per treatment) were slaughtered and the M...
June 26, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728382/effects-of-a-traditional-chinese-medicine-formula-and-its-extraction-on-muscle-fiber-characteristics-in-finishing-pigs-porcine-cell-proliferation-and-isoforms-of-myosin-heavy-chain-gene-expression-in-myocytes
#6
Qin Ping Yu, Ding Yuan Feng, Xiao Jun He, Fan Wu, Min Hao Xia, Tao Dong, Yi Hua Liu, Hui Ze Tan, Shi Geng Zou, Tao Zheng, Xian Hua Ou, Jian Jun Zou
Objective: This study evaluated the effects of a traditional Chinese medicine formula (TCMF) on muscle fiber characteristics in finishing pigs and the effects of the formula's extract (distilled water, ethyl acetate and petroleum ether extraction) on porcine cell proliferation and isoforms of MyHC gene expression in myocytes. Methods: XThe psoas major muscle was obtained from pigs at the end of the experiment. Muscle fiber characteristics in the psoas major muscle were analyzed using myosin ATPase staining...
June 26, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728371/expression-of-fox-related-genes-in-the-skin-follicles-of-inner-mongolia-cashmere-goat
#7
Wenjing Han, Xiaoyan Li, Lele Wang, Honghao Wang, Kun Yang, Zhixin Wang, Ruijun Wang, Rui Su, Zhihong Liu, Yanhong Zhao, Yanjun Zhang, Jinquan Li
Objective: The aim of this study was to investigate the expression of genes in cashmere goats at different periods of their fetal development. Methods: Bioinformatics analysis was used to evaluate data obtained by transcriptome sequencing of fetus skin samples collected from Inner Mongolia cashmere goats on days 45, 55, and 65 of fetal age. Results: We found that FoxN1, FoxE1, and FoxI3 genes of the Fox gene family were probably involved in the growth and development of the follicle and the formation of hair, which is consistent with previous findings...
June 27, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728370/endotoxin-induced-inflammation-disturbs-melatonin-secretion-in-ewe
#8
Andrzej Przemysław Herman, Karolina Wojtulewicz, Joanna Bochenek, Agata Krawczyńska, Hanna Antushevich, Bartosz Pawlina, Marlena Zielińska-Góska, Anna Herman, Katarzyna Romanowicz, Dorota Tomaszewska-Zaremba
The study was designed to determine the effect of intravenous administration of bacterial endotoxin - lipopolysaccharide (LPS) - on the nocturnal secretion of melatonin and on the expression of enzymes of the melatonin biosynthetic pathway in the pineal gland of ewes, taking into account two different photoperiodic conditions: short night (SN; n = 12) and long night (LN; n = 12). Endotoxin administration lowered (P < 0.05) levels of circulating melatonin in animals from LN photoperiod only during the first hour after treatment, while in ewes from SN photoperiod only in the third hour after the injection...
June 27, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728367/comparative-analysis-on-genome-wide-dna-methylation-in-longissimus-dorsi-muscle-between-small-tailed-han-and-dorper-%C3%A3-small-tailed-han-crossbred-sheep
#9
Yang Cao, Hai-Guo Jin, Hui-Hai Ma, Zhihui Zhao
Objective: The objective of this study was to compare the DNA methylation profile in the longissimus dorsi muscle (LDM) between Small Tailed Han and Dorper × Small Tailed Han crossbred sheep which were known to exhibit significant difference in meat-production. Methods: Six samples were subjected to MeDIP-seq and subsequent bioinformatics analyses to detect differentially methylated regions (DMRs) between the two groups. Results: 23.08 Gb clean data from six samples were generated and 808 DMRs were identified in gene body or their neighboring up/downstream regions...
June 27, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728363/effect-of-different-levels-of-protein-concentrates-supplementation-on-the-growth-performance-plasma-amino-acids-profile-and-mtor-cascade-genes-expression-in-early-weaned-yak-calves
#10
Quhani Peng, Nazir Ahmad Khan, B Xue, T H Yan, Z S Ahmad Wang
Objective: Yak is anone of the most important livestock species in the alpine-meadow grasslands of Qinghai-Tibetan plateau. WeThis study evaluated the effects of different levels of protein concentrate supplementation on the growth performance of yak calves, and correlated the growth rate to the changes occurring in the plasma- amino acids, and -insulin profile, and to the signaling activity of mammalian target of rapamycin (mTOR) cascade, in order to characterize the mechanism through which the protein synthesis can be improved in early weaned yaks...
June 27, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728340/screening-of-differential-proteins-of-placenta-tissues-in-patients-with-pre-eclampsia-by-itraq-proteomics-techniques
#11
Wei-Hong Qi, Ming-Yan Zheng, Chao Li, Lin Xu, Jin-E Xu
BACKGROUND: Isotopically labeled relative and absolute quantification (iTRAQ) were applied together with liquid chromatogram-tandem mass spectrometry (LC-MS/MS) technique to screen and differentiate normal tissues and differential proteins of placenta tissue in the pre-eclampsia group, and to seek for a pre-eclampsia biomarker. METHODS: Thirty patients with severe pre-eclampsia (pre-eclampsia group) and 30 patients with normal full-term pregnancy (control group), who were admitted by the Obstetrical Department of The Affiliated Hospital of Qingdao University from December 2014 to June 2015, were collected for this study...
October 2017: Minerva Medica
https://www.readbyqxmd.com/read/28728309/escherichia-coli-outer-membrane-protein-f-ompf-an-immunogenic-protein-induces-cross-reactive-antibodies-against-escherichia-coli-and-shigella
#12
Xiao Wang, Da Teng, Qingfeng Guan, Ruoyu Mao, Ya Hao, Xiumin Wang, Junhu Yao, Jianhua Wang
Diarrhea caused by pathogenic Escherichia coli (E. coli) is one of the most serious infectious diseases in humans and animals. Due to antibiotics resistance and the lack of efficient vaccine, more attention should be paid to find potential versatile vaccine candidates to prevent diseases. In this study, the sequence homology analysis indicated that OmpF from E. coli CVCC 1515 shares a high identity (90-100%) with about half of the E. coli (46.7%) and Shigella (52.8%) strains. Then the recombinant OmpF was supposed to be developed as a versatile vaccine to prevent E...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28728298/-the-prognostic-analysis-of-hepatocellular-carcinoma-based-on-the-tumor-neo-vessels-macrophages-and-%C3%AE-sma-in-tumor-microenvironment
#13
M Fang, J P Yuan, L L Liu, G P Cheng, H J Ying, Y M Chen, M Chen
Objective: To analyze the quantitative expression and prognostic significance of tumor neo-vessels, macrophages and fibroblasts in tumor microenvironment of hepatocellular carcinoma (HCC). Methods: The clinic-pathological features and tissue samples for 101 HCC cases were collected. Immunohistochemistry was used to stain the tumor neo-vessels, macrophages and fibroblasts on tumor tissue. The distribution results and quantitative data of above key components were acquired by inverted microscopy equipped with CRi Nuance multispectral analysis system...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28728293/-curcumin-induces-apoptosis-and-protective-autophagy-in-human-gastric-cancer-cells-with-different-degree-of-differentiation
#14
W Li, Y Zhou, J Yang, H H Zhang, S L Zhao, T Zhang, J Huo, P Zheng
Objective: To investigate the effect of curcumin on the apoptosis and autophagy of human gastric cancer cells with different degree of differentiation. Methods: Gastric cancer cell lines BGC-823 and MKN-28 were treated with curcumin at different concentrations. The effect of curcumin on cell proliferation was measured by MTT assay. Apoptosis was assessed by flow cytometry. Autophagy status was analyzed by acridine orange staining. The expression levels of apoptotic and autophagy-related proteins were detected by Western blot...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28728292/-detection-and-clinical-significance-of-circulating-tumor-cells-in-osteosarcoma-using-immunofluorescence-combined-with-in-situ-hybridization
#15
H Q Zhang, M H Li, Z Wang, P H Lan, Y J Lu, G J Chen, L Wang
Objective: To investigate the clinical significance of detection of circulating tumor cells (CTCs) in peripheral blood from patients with osteosarcoma (OS) using the iFISH (immunofluorescence and fluorescence in situ hybridization) method. Methods: The live cells recovery rate of immune-magnetic beads was evaluated by live-cell fluorescent tracer technology. The expression of CD45 and CK18 on the cell surface of HOS and HepG2 cells was measured by flow cytometry. And the chromosome aneuploidy was detected by centromeric FISH probe CEP8...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28728289/-the-protective-effect-of-pigment-epithelial-derived-factor-modified-human-umbilical-cord-mesenchymal-stem-cells-on-rats-with-diabetic-retinopathy
#16
W Zhang, H T Duan, S Chen, Y X Wang, J H Kong, M Dong, X Bi, J Song
Objective: To investigate the effect of pigment epithelial-derived factor (PEDF) gene-modified human umbilical cord mesenchymal stem cells (MSC) on rats with diabetic retinopathy (DR). Methods: Experimental study. Human umbilical cord MSC were transfected by lentivirus packaging PEDF-MSC-green fluorescent protein (GFP) and GFP-MSC plasmid vectors, and the expression of PEDF and vascular endothelial growth factor (VEGF) was measured in the cell culture medium. Fifty adult male Sprague-Dawley rats were randomly divided into five groups: normal control group (group A), DR control group (group B), phosphate-buffered saline (PBS) treated group (group C), GFP-MSC treated group (group D) and PEDF-MSC-GFP treated group (group E), with 10 rats in each group...
July 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28728277/-effects-of-pi3k%C3%AE-rhoa-pathway-on-phagocytosis-defect-of-alveolar-macrophages-in-a-mouse-model-of-chronic-obstructive-pulmonary-disease
#17
S T Hu, Q Xia, X L Zeng, H R Bao, X J Liu
Objective: To investigate effects of Phosphoinositide3-Kinases (PI3Kδ)-Ras homolog gene family member A(RhoA) pathway on phagocytosis deficiency of alveolar macrophages (AMs) in a mouse model of chronic obstructive pulmonary disease (COPD). Methods: Twenty mice were exposed to cigarette smoking to establish the COPD model, with 20 mice as the control group. AMs were isolated from lung tissue by discontinuous density gradient centrifugation and then divided into a healthy control group, a COPD group, a healthy IC87114 group and a COPD IC87114 group...
July 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28728276/-the-role-of-hur-in-mediating-snail-expression-in-human-small-airway-epithelium-induced-by-cigarette-smoke-extract
#18
X M Gu, X G Wang, J Sun, N Wang, S J Jiang
Objective: To investigate the abundance of human antigen R (HuR) in small airway epithelial cells stimulated by cigarette smoke extract (CSE) as well as the role of HuR in mediating snail which is recognized as a key transcription factor in regulating epithelial-mesenchymal transition (EMT). Methods: Human small airway epithelial cells (HSAEpiC) cultured in vitro were exposed to cigarette smoke extract (CSE) to model COPD status. Real-time PCR and western blotting analysis were used in detecting HuR protein and mRNA expression in cells with CSE which were divided into 5 groups: a control group, a 1%-24 h group, a 3%-24 h group, a 1%-48 h group and a 3%-48 h group...
July 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28728275/-changes-and-differences-of-dna-methylation-in-human-macrophages-infected-with-virulent-and-avirulent-mycobacterium-tuberculosis
#19
L N Lyu, H Y Jia, Z H Li, Z Q Liu, Z D Zhang
Objective: To profile DNA methylation and compare differentially methylated region (DMR) of macrophages infected with virulent and avirulent strains of Mycobacterium tuberculosis (MTB). Methods: PMA(50 ng/ml) treated THP-1 macrophages were left uninfected or infected with the virulent H37Rv(THP-1/Rv) or avirulent H37Ra(THP-1/Ra). The genomic DNA was then extracted and DNA methylation was profiled via Reduced Representation Bisulfite Sequencing (RRBS). The DNA methylation pattern and DMRs between the tested samples were indentified...
July 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28728242/-expression-and-significance-of-pepsin-in-lingual-tonsil-hypertrophy
#20
Y Q Deng, L Wang, H H Chen, J J Tan, C K Gao, X X Huang, X Y Han, X P Li
Objective: To discuss the relationship between lingual tonsil hypertrophy and laryngopharyngeal reflux. Methods: Ninety-two patients who received throat surgery in Nanfang Hospital between October 2015 and October 2016 were enrolled. Twenty-six healthy volunteers were recruited as normal controls. All participants were assessed with the reflux finding score(RFS) and the size of lingual tonsils were evaluated using a clinical grading system proposed by Friedman under electronic laryngoscope. The score of reflux symptom index(RSI), personal history and medical history were gathered...
July 7, 2017: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
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