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Amino acids

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https://www.readbyqxmd.com/read/29475157/the-role-of-the-n-terminal-domain-of-human-apurinic-apyrimidinic-endonuclease-1-ape1-in-dna-glycosylase-stimulation
#1
Olga A Kladova, Milena Bazlekowa-Karaban, Sonia Baconnais, Olivier Piétrement, Alexander A Ishchenko, Bakhyt T Matkarimov, Danila A Iakovlev, Andrey Vasenko, Olga S Fedorova, Eric Le Cam, Barbara Tudek, Nikita A Kuznetsov, Murat Saparbaev
The base excision repair (BER) pathway consists of sequential action of DNA glycosylase and apurinic/apyrimidinic (AP) endonuclease necessary to remove a damaged base and generate a single-strand break in duplex DNA. Human multifunctional AP endonuclease 1 (APE1, a.k.a. APEX1, HAP-1, or Ref-1) plays essential roles in BER by acting downstream of DNA glycosylases to incise a DNA duplex at AP sites and remove 3'-blocking sugar moieties at DNA strand breaks. Human 8-oxoguanine-DNA glycosylase (OGG1), methyl-CpG-binding domain 4 (MBD4, a...
February 11, 2018: DNA Repair
https://www.readbyqxmd.com/read/29475074/stapled-truncated-orexin-peptides-as-orexin-receptor-agonists
#2
Lasse Karhu, Janne Weisell, Pauli M Turunen, Teppo O Leino, Henri Pätsi, Henri Xhaard, Jyrki P Kukkonen, Erik A A Wallén
The peptides orexin-A and -B, the endogenous agonists of the orexin receptors, have similar 19-amino-acid C-termini which retain full maximum response as truncated peptides with only marginally reduced potency, while further N-terminal truncations successively reduce the activity. The peptides have been suggested to bind in an α-helical conformation, and truncation beyond a certain critical length is likely to disrupt the overall helical structure. In this study, we set out to stabilize the α-helical conformation of orexin-A15-33 via peptide stapling at four different sites...
February 20, 2018: Peptides
https://www.readbyqxmd.com/read/29475030/screening-for-protein-adducts-of-naphthalene-and-chrysene-in-plasma-of-exposed-atlantic-cod-gadus-morhua
#3
Karianne S Enerstvedt, Magne O Sydnes, Eivind Larssen, Daniela M Pampanin
Polycyclic aromatic hydrocarbons (PAHs) are well known contaminants, ubiquitously present in the habitat and spawning areas for Atlantic cod (Gadus morhua). The Atlantic cod is a key species and a globally important food source, thus continuous monitoring of PAHs is considered highly valuable to ensure ecosystem sustainability and human food safety. PAH adducts to plasma proteins are applied as sensitive biomarkers of PAH exposure in humans and other species, thus the presence of PAH protein adducts in Atlantic cod plasma was investigated to identify PAH protein adduct biomarker candidates of exposure to PAHs...
February 16, 2018: Chemosphere
https://www.readbyqxmd.com/read/29474999/serum-metabolomics-study-in-a-group-of-parkinson-s-disease-patients-from-northern-india
#4
G Nagesh Babu, Manjeet Gupta, Vimal K Paliwal, Suruchi Singh, Tanushri Chatterji, Raja Roy
BACKGROUND: Parkinson's disease (PD) is the result of progressive degeneration of the nigrostriatal dopaminergic pathway and depletion of neurotransmitter dopamine in the striatum. METHODS: We included 17 patients with PD along with 7 patients of progressive supranuclear palsy (PSP), 6 patients of multiple system atrophy (MSA) and 22 age and sex-matched healthy controls. We analyzed metabolite profiles in the serum of these patients and controls using1 H NMR spectroscopy...
February 20, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29474755/manganese-catalyzed-carbonylative-annulations-for-redox-neutral-late-stage-diversification
#5
Yu-Feng Liang, Ralf Steinbock, Annika Münch, Dietmar Stalke, Lutz Ackermann
An inexpensive, non-toxic manganese catalyst enabled unprecedented redox-neutral carbonylative annulations under ambient pressure. The manganese catalyst outperformed all other typically used base and precious metal catalysts. The outstanding versatility of the manganese catalysis manifold was reflected by ample substrate scope, setting the stage for effective late-stage manipulations under racemization-free conditions on a wealth of marketed drugs and natural products, including alkaloids, amino acids, steroids and carbohydrates...
February 23, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29474728/longitudinal-changes-in-blood-metabolites-amino-acid-profile-and-oxidative-stress-markers-in-american-foxhounds-fed-a-nutrient-fortified-diet
#6
Alison N Beloshapka, Maria R C de Godoy, Rebecca A Carter, Andrea J Fascetti, Zengshou Yu, Bridgett J McIntosh, Kelly S Swanson, Preston R Buff
The objective of the present study was to evaluate the changes in blood metabolites, amino acid profile, and oxidative stress markers in American Foxhound dogs fed a nutrient-fortified endurance diet while undergoing unstructured endurance exercise over several months. Thirty-six adult American Foxhound dogs (mean age: 4.5, range 2-10 years and mean body weight (BW): 34.7, range: 23.1-46.9 kg) were selected to participate in the study. Prior to the study, all dogs consumed a commercial diet for 16 weeks. After collecting baseline blood samples, dogs were assigned to a standard commercial performance diet (control) or a nutrient-fortified dog food (test)...
February 21, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29474620/a-conserved-leucine-zipper-like-motif-accounts-for-strong-tetramerization-capabilities-of-sepallata-like-mads-domain-transcription-factors
#7
Florian Rümpler, Günter Theißen, Rainer Melzer
The development of angiosperm flowers is regulated by homeotic MIKC-type MADS-domain transcription factors that activate or repress target genes via the formation of DNA-bound, organ specific tetrameric complexes. The protein-protein interaction (PPI) capabilities differ considerably between different MIKC-type proteins. The floral homeotic protein SEPALLATA3 (SEP3) acts as a hub that incorporates numerous other MADS-domain proteins into tetrameric complexes that would otherwise not form. However, the molecular mechanisms that underlie these promiscuous interactions remain largely unknown...
February 21, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29474495/in-silico-and-in-vitro-studies-of-the-reduction-of-unsaturated-%C3%AE-%C3%AE-bonds-of-trans-2-hexenedioic-acid-and-6-amino-trans-2-hexenoic-acid-important-steps-towards-biobased-production-of-adipic-acid
#8
Emma Karlsson, Jae Ho Shin, Gunnar Westman, Leif A Eriksson, Lisbeth Olsson, Valeria Mapelli
The biobased production of adipic acid, a precursor in the production of nylon, is of great interest in order to replace the current petrochemical production route. Glucose-rich lignocellulosic raw materials have high potential to replace the petrochemical raw material. A number of metabolic pathways have been proposed for the microbial conversion of glucose to adipic acid, but achieved yields and titers remain to be improved before industrial applications are feasible. One proposed pathway starts with lysine, an essential metabolite industrially produced from glucose by microorganisms...
2018: PloS One
https://www.readbyqxmd.com/read/29474447/contribution-of-s4-segments-and-s4-s5-linkers-to-the-low-voltage-activation-properties-of-t-type-cav3-3-channels
#9
Ana Laura Sanchez-Sandoval, Zazil Herrera Carrillo, Clara Estela Díaz Velásquez, Dulce María Delgadillo, Heriberto Manuel Rivera, Juan Carlos Gomora
Voltage-gated calcium channels contain four highly conserved transmembrane helices known as S4 segments that exhibit a positively charged residue every third position, and play the role of voltage sensing. Nonetheless, the activation range between high-voltage (HVA) and low-voltage (LVA) activated calcium channels is around 30-40 mV apart, despite the high level of amino acid similarity within their S4 segments. To investigate the contribution of S4 voltage sensors for the low-voltage activation characteristics of CaV3...
2018: PloS One
https://www.readbyqxmd.com/read/29474444/epitopes-for-neutralizing-antibodies-induced-by-hiv-1-envelope-glycoprotein-bg505-sosip-trimers-in-rabbits-and-macaques
#10
P J Klasse, Thomas J Ketas, Christopher A Cottrell, Gabriel Ozorowski, Gargi Debnath, Diawoye Camara, Erik Francomano, Pavel Pugach, Rajesh P Ringe, Celia C LaBranche, Marit J van Gils, Christine A Bricault, Dan H Barouch, Shane Crotty, Guido Silvestri, Sudhir Kasturi, Bali Pulendran, Ian A Wilson, David C Montefiori, Rogier W Sanders, Andrew B Ward, John P Moore
The native-like, soluble SOSIP.664 trimer based on the BG505 clade A env gene of HIV-1 is immunogenic in various animal species, of which the most studied are rabbits and rhesus macaques. The trimer induces autologous neutralizing antibodies (NAbs) consistently but at a wide range of titers and with incompletely determined specificities. A precise delineation of immunogenic neutralization epitopes on native-like trimers could help strategies to extend the NAb response to heterologous HIV-1 strains. One autologous NAb epitope on the BG505 Env trimer is known to involve residues lining a hole in the glycan shield that is blocked by adding a glycan at either residue 241 or 289...
February 23, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29474408/molecular-adaptations-to-phosphorus-deprivation-and-comparison-with-nitrogen-deprivation-responses-in-the-diatom-phaeodactylum-tricornutum
#11
Leila Alipanah, Per Winge, Jens Rohloff, Javad Najafi, Tore Brembu, Atle M Bones
Phosphorus, an essential element for all living organisms, is a limiting nutrient in many regions of the ocean due to its fast recycling. Changes in phosphate (Pi) availability in aquatic systems affect diatom growth and productivity. We investigated the early adaptive mechanisms in the marine diatom Phaeodactylum tricornutum to P deprivation using a combination of transcriptomics, metabolomics, physiological and biochemical experiments. Our analysis revealed strong induction of gene expression for proteins involved in phosphate acquisition and scavenging, and down-regulation of processes such as photosynthesis, nitrogen assimilation and nucleic acid and ribosome biosynthesis...
2018: PloS One
https://www.readbyqxmd.com/read/29473933/assessment-of-genetic-integrity-splenic-phagocytosis-and-cell-death-potential-of-z-4-1-5-dimethyl-3-oxo-2-phenyl-2-3dihydro-1h-pyrazol-4-yl-amino-4-oxobut-2-enoic-acid-and-its-effect-when-combined-with-commercial-chemotherapeutics
#12
Rodrigo Juliano Oliveira, Naiara da Cruz Leite Santos, João Renato Pesarini, Beatriz Carneiro de Oliveira, Claudia Rodrigues Berno, Flávio Henrique Souza de Araújo, Ingridhy Ostaciana Maia Freitas da Silveira, Raquel Oliveira Nascimento, Andréia Conceição Milan Brochado Antoniolli-Silva, Antônio Carlos Duenhas Monreal, Adilson Beatriz, Dênis Pires de Lima, Roberto da Silva Gomes
The increased incidence of cancer and its high treatment costs have encouraged the search for new compounds to be used in adjuvant therapies for this disease. This study discloses the synthesis of (Z)-4-((1,5-dimethyl-3-oxo-2-phenyl-2,3dihydro-1H-pyrazol-4-yl) amino)-4-oxobut-2-enoic acid (IR-01) and evaluates not only the action of this compound on genetic integrity, increase in splenic phagocytosis and induction of cell death but also its effects in combination with the commercial chemotherapeutic agents doxorubicin, cisplatin and cyclophosphamide...
February 19, 2018: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/29473887/characterization-stability-and-biological-activity-in-vitro-of-cathelicidin-bf-30-loaded-4-arm-star-shaped-peg-plga-microspheres
#13
Yueli Bao, Shanrong Wang, Hongli Li, Yunjiao Wang, Haiyun Chen, Minglong Yuan
BF-30 is a single chain polypeptide of an N-segment with an α-helix from cathelicidin gene encoding, and it contains 30 amino acid residues, with a relative molecular mass and isoelectric point of 3637.54 and 11.79, respectively. Cathelicidin-BF-30 was entrapped in four-arm star-shaped poly(ethylene glycol-b-dl-lactic acid-co-glycolic acid) block copolymers (4-arm-PEG-PLGA) by a double-emulsion solvent-evaporation method. Three release phases of cathelicidin-BF-30loaded 4-arm-PEG-PLGA microspheres were observed, including an initial burst-release phase, followed by a lag phase with minimal drug release and finally a secondary zero-order release phase...
February 23, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29473879/in-silico-based-repositioning-of-phosphinothricin-as-a-novel-technetium-99m-imaging-probe-with-potential-anti-cancer-activity
#14
Tamer M Sakr, Mohammed A Khedr, Hassan M Rashed, Maged E Mohamed
l-Phosphinothricin (glufosinate or 2-amino-4-((hydroxy(methyl) phosphinyl) butyric acid ammonium salt (AHPB)), which is a structural analog of glutamate, is a recognized herbicide that acts on weeds through inhibition of glutamine synthetase. Due to the structural similarity between phosphinothricin and some bisphosphonates (BPs), this study focuses on investigating the possibility of repurposing phosphinothricin as a bisphosphonate analogue, particularly in two medicine-related activities: image probing and as an anti-cancer drug...
February 23, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29473734/ligand-size-and-conformation-affect-the-behavior-of-nanoparticles-coated-with-in-vitro-and-in-vivo-protein-corona
#15
Huajin Zhang, Tianmu Wu, Wenqi Yu, Shaobo Ruan, Qin He, Huile Gao
Protein corona is immediately established on the surface of nanoparticles upon their introduction into biological milieu. Several studies have shown that the targeting efficiency of ligand-modified nanoparticles is attenuated or abolished, owing to the protein adsorption. Here, transferrin receptor-targeting ligands, including LT7 (CHAIYPRH), DT7 (hrpyiahc, all D-form amino acids), and transferrin, were used to identify the influence of the ligand size and conformation on protein corona formation. The results showed that the targeting capacity of ligand-modified nanoparticles was lost after incubation with plasma in vitro, while it was partially retained after in vivo corona formation...
February 23, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29473729/streptomyces-wadayamensis-mppp-is-a-plp-dependent-oxidase-not-an-oxygenase
#16
Lanlan Han, Nemanja Vuksanovic, Sarah A Oehm, Tyler Grant Fenske, Alan W Schwabacher, Nicholas Silvaggi
The PLP-dependent L-arginine hydroxylase/deaminase MppP from Streptomyces wadayamensis (SwMppP) is involved in the biosynthesis of L-enduracididine, a nonproteinogenic amino acid found in a number of nonribosomally produced peptide antibiotics. SwMppP uses only PLP and molecular oxygen to catalyze a 4-electron oxidation of L-arginine to form a mixture of 2-oxo-4(S)-hydroxy-5-guanidinovaleric acid and 2-oxo-5-guanidinovaleric acid. Steady state kinetics analysis in the presence and absence of catalase show that one molecule of peroxide is formed for every molecule of dioxygen consumed in the reaction...
February 23, 2018: Biochemistry
https://www.readbyqxmd.com/read/29473702/dual-c-sp3-h-bond-functionalization-of-n-heterocycles-via-visible-light-photocatalyzed-dehydrogenation-2-2-cycloaddition-sequential-reaction
#17
Guo-Qiang Xu, Ji-Tao Xu, Zhi-Tao Feng, Hui Liang, Zhu-Yin Wang, Yong Qin, Peng-Fei Xu
Here we describe a mild method to achieve dual C(sp3)-H bond functionalization of saturated nitrogen-containing heterocycles via a sequential visible-light photocatalyzed dehydrogenation/[2+2] cycloaddition procedure. As a complementary approach to the well-established iminium ion and α-amino radical intermediates, the elusive cyclic enamine intermediates are effectively generated via photoredox catalysis under mild conditions, which are efficiently captured by acetylene ester to form a wide array of bicyclic amino acid derivatives and realize the simultaneous functionalization of two vicinal C(sp3)-H bonds...
February 23, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29473490/identification-and-analysis-of-key-residues-involved-in-folding-and-binding-of-protein-carbohydrate-complexes
#18
Michael Gromiha, N R Siva Shanmugam, J Fermin Angelo Selvin, K Veluraja
Protein-carbohydrate interactions play vital roles in several biological processes in living organisms. The comparative analysis of binding site residues along with stabilizing residues in protein-carbohydrate complexes provides ample insights to understand the structure, function and recognition mechanism. In this work, we have identified 2.45% binding site residues in a non-redundant dataset of 1130 complexes using distance-based criteria and 7.07% stabilizing residues using the concepts of hydrophobicity, long-range interactions and conservation of residues...
February 21, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29473382/a-randomized-multicenter-controlled-study-comparing-efficacy-and-safety-of-a-new-complementary-and-alternative-medicine-cam-versus-solifenacin-succinate-in-women-with-overactive-bladder-syndrome
#19
Carlo Vecchioli-Scaldazza, Carolina Morosetti, Serena Maruccia, Stefano Casellato, Wilma Rociola, Ester Illiano, Francesco Garofalo
INTRODUCTION: To assess efficacy and tolerability of a new complementary and alternative medicine (CAM) consisting of vitamins (C and D), herbal products (cucurbita maxima, capsicum annum, polygonum capsicatum) and amino acid L-Glutammina, in the treatment of female Overactive Bladder syndrome (OAB). MATERIALS AND METHODS: 90 consecutive women with OAB symptoms were enrolled in this prospective, randomized, controlled study. Women were divided randomly into two groups of 45 patients each...
December 31, 2017: Archivio Italiano di Urologia, Andrologia
https://www.readbyqxmd.com/read/29473327/epidemiological-investigation-of-pseudorabies-in-shandong-province-from-2013-to-2016
#20
J Gu, D Hu, T Peng, Y Wang, Z Ma, Z Liu, F Meng, Y Shang, S Liu, Y Xiao
In late 2011, a variant pseudorabies virus (vPRV) emerged in Bartha-K61-vaccinated pig herds, resulting in high morbidity and mortality of piglets in China. Since 2013, the autopsy lesions, histological examinations, virus isolation, phylogenetic analysis and selection pressure analysis of the gE gene of vPRV were recorded for 395 clinical cases, and 5,033 pig serum samples were detected by PRV gE-coated enzyme-linked immunosorbent assay. The major clinical symptoms were abortion in pregnant sows, fatal neurological signs in piglets and respiratory disease in growing pigs...
February 22, 2018: Transboundary and Emerging Diseases
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