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https://www.readbyqxmd.com/read/28823130/use-of-alternative-curing-salts-for-processing-salamis
#1
Dong-Gyun Yim, Ku-Young Chung, Cheorun Jo, Ki-Chang Nam
Objective: This study was performed to determine effects of different curing salts on the quality of salamis and to assess feasibility of using NaCl-alternative salts. Methods: Various types of curing salts (KCl or MgCl2) as well as NaCl (sun-dried or refined) were incorporated for processing of salamis. The proximate composition, fatty acids, nucleotide-related compounds, and free amino acids of the salamis were analyzed during 40 days of ripening. Results: The substitution of NaCl by KCl caused higher fat and ash content, but lower moisture content of the salami after 20 days of ripening (p<0...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28823128/molecular-cloning-and-expression-analysis-of-annexin-a2-gene-in-sika-deer-antler-tip
#2
Yanling Xia, Haomiao Qu, Binshan Lu, Qiang Zhang, Heping Li
Objective: Molecular cloning and bioinformatics analysis of Annexin A2 gene in sika deer antler tip were conducted. The role of Annexin A2 gene in the growth and development of the antler were analyzed initially. Methods: The reverse transcriptase-polymerase chain reaction (RT-PCR) was used to clone the cDNA sequence of the Anxa2 gene from antler tip of sika deer (Cervus nippon hortulorum) and the bioinformatics tools provided on the web site were applied to analyze the amino acid sequence of Anxa2 protein...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28823126/current-status-and-prospective-of-in-feed-additive-antibiotics-in-the-individual-stage-of-the-pork-production
#3
Junyou Li
Antibiotics have long been of great benefit for people, both in the medical treatment of human disease and in animal food that improves the growth performance and feed utilization during animal production. Antibiotics as in-feed supplements affect all stages of pork production, including the gestation, nursing, growing, and finishing stages, although the effects show stage-dependent differences. However, the use of antibiotics in animal feed has become a worldwide concern. This review describes why sub-therapeutic levels of antibiotic additives in animal feed have become an integral part of animal feeding programs for more than 70 years, particularly in pork production...
August 16, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28823097/epitope-mapping-of-histo-blood-group-antigens-bound-to-norovirus-vlps-using-std-nmr-experiments-reveals-fine-details-of-molecular-recognition
#4
Brigitte Fiege, Mila Leuthold, Francisco Parra, Kevin P Dalton, Peter J Meloncelli, Todd L Lowary, Thomas Peters
Attachment of human noroviruses to histo blood group antigens (HBGAs) is thought to be critical for the infection process. Therefore, we have determined binding epitopes of synthetic type 1 to 6 blood group A- and B-tetrasaccharides binding to GII.4 human Norovirus virus like particles (VLPs) using STD NMR experiments. So far, little information is available from crystal structure analysis studies on the interactions of the reducing-end sugars with the protruding domain (P-domain) of the viral coat protein VP1...
August 19, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28822954/the-variability-of-hepatitis-b-envelope-is-associated-with-hbs-antigen-persistence-in-either-chronic-or-acute-hbv-genotype-a-infection
#5
Marine Eschlimann, Brice Malvé, Aurélie Velay, Honorine Fenaux, Sibel Berger, Jean-Pol Frippiat, Fabien Zoulim, Mouni Bensenane, Jean-Pierre Bronowicki, François Goehringer, Thierry May, Hélène Jeulin, Evelyne Schvoerer
BACKGROUND: More than 240 million people are chronically infected by hepatitis B virus (HBV) worldwide. Envelope proteins play a crucial role in viral cellular entry and immune recognition. The loss of HBs antigen (HBsAg) correlated with a good clinical prognosis is rarely achieved with or without treatment (3-16%). OBJECTIVES: HBV envelope variability was investigated according to HBsAg persistence. STUDY DESIGN: The cohort consisted of 15 HBV genotype A-infected patients divided into "resolvers", with HBsAg clearance, and "non-resolvers", with HBsAg persistence and in subgroups: acute (n=5, AHBV) or chronic infection (n=4, CHBV) and HBV/HIV coinfection (n=6, CHBV/HIV)...
August 5, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28822940/effects-of-airborne-ammonium-and-nitrate-pollution-strongly-differ-in-peat-bogs-but-symbiotic-nitrogen-fixation-remains-unaffected
#6
Eva van den Elzen, Leon J L van den Berg, Bas van der Weijden, Christian Fritz, Lucy J Sheppard, Leon P M Lamers
Pristine bogs, peatlands in which vegetation is exclusively fed by rainwater (ombrotrophic), typically have a low atmospheric deposition of reactive nitrogen (N) (<0.5kgha(-1)y(-1)). An important additional N source is N2 fixation by symbiotic microorganisms (diazotrophs) in peat and mosses. Although the effects of increased total airborne N by anthropogenic emissions on bog vegetation are well documented, the important question remains how different N forms (ammonium, NH4(+), versus nitrate, NO3(-)) affect N cycling, as their relative contribution to the total load strongly varies among regions globally...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822928/combined-effects-of-salinity-temperature-and-hypoxia-on-daphnia-magna-metabolism
#7
Elba Garreta-Lara, Bruno Campos, Carlos Barata, Silvia Lacorte, Romà Tauler
Metabolic changes of Daphnia magna pools due different abiotic factors linked to global climate change (salinity, temperature and hypoxia) were investigated using untargeted GC-MS and advanced chemometric strategies using a three factors two-level full factorial experimental design (DoE). Effects of these three factors and identity of the metabolites whose concentrations changed because of them were investigated. The simultaneous analysis of GC-MS data sets using Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) allowed the resolution of the elution and mass spectra profiles of a large number of D...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822890/production-of-a-novel-monoclonal-antibody-applicable-for-an-immunochromatographic-assay-for-kudoa-septempunctata-spores-contaminating-the-raw-olive-flounder-paralichthys-olivaceus
#8
Michio Jinnai, Takao Kawai, Tetsuya Harada, Yasutaka Nishiyama, Hiroshi Yokoyama, Sho Shirakashi, Hiroshi Sato, Junko Sakata, Yuko Kumeda, Yutaka Fukuda, Kikuyo Ogata, Kentaro Kawatsu
Kudoa septempunctata, a myxosporean parasite of the olive flounder (Paralichthys olivaceus), causes foodborne gastroenteritis after ingestion of contaminated raw flounder. Available methods to detect K. septempunctata require expensive equipment, well-trained personnel, and lengthy procedures. Here we generated a novel monoclonal antibody (MAb 15G11) against K. septempunctata and used it to produce a prototype immunochromatographic assay (prototype Kudoa-ICA). Within 15min, the prototype Kudoa-ICA detected ≥1...
August 9, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28822547/bacterial-diversity-in-goat-milk-from-the-guanzhong-area-of-china
#9
Fuxin Zhang, Zhaoxia Wang, Feiyan Lei, Bini Wang, Shuaiming Jiang, Qiannan Peng, Jiachao Zhang, Yuyu Shao
In this study, the V3 and V4 regions of the 16S rRNA gene from metagenomic DNA were sequenced to identify differences in microbial diversity in raw milk of Saanen and Guanzhong goats from the Guanzhong area of China. The results showed that Proteobacteria was the predominant phylum, accounting for 71.31% of all phyla identified in milk from the 2 breeds, and Enterobacter was the predominant genus (24.69%) within the microbial community. Microbial alpha diversity from Saanen goat milk was significantly higher than that of Guanzhong goat milk based on bioinformatic analysis of indices of Chao1, Shannon, Simpson, observed species, and the abundance-based coverage estimator...
August 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28822346/comparative-study-of-single-combination-use-of-huang-lian-jie-du-decoction-and-berberine-on-their-protection-on-sepsis-induced-acute-liver-injury-by-nmr-metabolic-profiling
#10
Yan Lv, Junsong Wang, Dingqiao Xu, Shanting Liao, Pei Li, Qian Zhang, Minghua Yang, Lingyi Kong
Sepsis is a serious clinical disease with a high mortality rate all around the world. Liver organ dysfunction is an important sign for the severity and outcome of sepsis in patients. In this study, (1)H NMR-based metabolomics approach and biochemical assays were applied to investigate the metabolic profiling for cecal ligation and puncture (CLP) induced acute liver injury, the therapeutical effect of single/combination use of Huang-Lian-Jie-Du decoction (HLJDD) and berberine, and the interaction of them. Metabolomics analysis revealed significant perturbations in livers of septic rats, which could be ameliorated by HLJDD, berberine and their combination treatment...
August 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28822339/effects-of-simulated-n-deposition-on-foliar-nutrient-status-n-metabolism-and-photosynthetic-capacity-of-three-dominant-understory-plant-species-in-a-mature-tropical-forest
#11
Qinggong Mao, Xiankai Lu, Hui Mo, Per Gundersen, Jiangming Mo
Anthropogenic increase of nitrogen (N) deposition has threatened forest ecosystem health at both regional and global scales. In N-limited ecosystems, atmospheric N input is regarded as an important nutrient source for plant growth. However, it remains an open question on how elevated N deposition affects plant growth in N-rich forest ecosystems. To address this question, we used a simulated N deposition experiment in an N-rich mature tropical forest of southern China, with N addition levels as 0kgNha(-1)yr(-1) (Control), 50kgNha(-1)yr(-1) (Low-N), 100kgNha(-1)yr(-1) (Middle-N) and 150kgNha(-1)yr(-1) (High-N), respectively...
August 16, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822244/new-insights-into-the-enhanced-performance-of-high-solid-anaerobic-digestion-with-dewatered-sludge-by-thermal-hydrolysis-organic-matter-degradation-and-methanogenic-pathways
#12
Sisi Chen, Ning Li, Bin Dong, Wentao Zhao, Lingling Dai, Xiaohu Dai
Two lab-scale high solid anaerobic digesters fed with untreated sludge (R1) and thermally hydrolyzed sludge (R2) were operated to investigate the influence of thermal hydrolysis pretreatment (THP) on the degradation of individual macromolecular organic components (MOCs), as well as the functional and metabolic responses of microbes during anaerobic digestion (AD). The degradation of MOCs was improved by THP at different rates, in which improved degradation of proteins (by 49.0%) and hemicelluloses (by 25.0%) were the main factors contributing to the increase in volatile solids (VS) reduction...
August 8, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28822163/-mechanism-of-treatment-effect-of-huanglian-huangqin-herb-pairs-on-cerebral-ischemia-rats-based-on-metabolomic-approach
#13
Hui-Ting Cao, Hua-Xu Zhu, Qi-Chun Zhang, Li-Wei Guo
The metabolic effect of Huanglian-Huangqin herb pairs on cerebral ischemia rats was studied by using metabolomic method. The rat model of ischemia reperfusion injury induced by introduction of transient middle cerebral artery occlusion (MCAO) followed by reperfusion. Ultra high performance liquid chromatography-series four pole time of flight mass spectrometry method(UPLC-Q-TOF/MS), Markerlynx software, and principal component analysis and partial least-squares discriminant analysis were used to analyze the different endogenous metabolites among the urine samples of sham rats, cerebral ischemia model rats, Huanglian groups (HL), Huangqin groups (HQ) and Huanglian-Huangqin herb pairs groups (LQ) was achieved, combined with accurate information about the endogenous metabolites level and secondary fragment ions, retrieval and identification of possible biological markers, metabolic pathway which build in MetPA database...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822147/-research-on-chemotaxis-response-of-alternaria-panax-to-amino-acid-of-ginseng-root-exudates
#14
Ai-Hua Zhang, Wen-Li Ma, Feng-Jie Lei, Ning-Bo An, Xin-Xin Zhang, Zhi-Qing Liu, Lian-Xue Zhang
Plate assay and spore germination method were used to study the chemotaxis response of Alternaria panax to arginine, glutamic acid, aspartic acid and threonine. The result showed that the optimum temperature of A. panax chemotaxis response to four amino acids were all 25 ℃. And chemotaxis responses of A. panax were different under conditions of different concentration and pH value. The chemotaxin reached to the highest under the condition of 2 mg•L⁻¹ and pH value was 7 for arginine, glutamic acid and threonine while 20 mg•L⁻¹ and pH value was 6 for aspartic acid ...
June 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28822137/studying-the-function-of-the-phosphorylated-pathway-of-serine-biosynthesis-in-arabidopsis-thaliana
#15
Stephan Krueger, Ruben M Benstein, Sabine Wulfert, Armand D Anoman, María Flores-Tornero, Roc Ros
Photorespiration is an essential pathway in photosynthetic organisms and is particularly important to detoxify and recycle 2-phosphoglycolate (2-PG), a by-product of oxygenic photosynthesis. The enzymes that catalyze the reactions in the photorespiratory core cycle and closely associated pathways have been identified; however, open questions remain concerning the metabolic network in which photorespiration is embedded. The amino acid serine represents one of the major intermediates in the photorespiratory pathway and photorespiration is thought to be the major source of serine in plants...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28822133/isotopically-nonstationary-metabolic-flux-analysis-inst-mfa-of-photosynthesis-and-photorespiration-in-plants
#16
Fangfang Ma, Lara J Jazmin, Jamey D Young, Doug K Allen
Photorespiration is a central component of photosynthesis; however to better understand its role it should be viewed in the context of an integrated metabolic network rather than a series of individual reactions that operate independently. Isotopically nonstationary (13)C metabolic flux analysis (INST-MFA), which is based on transient labeling studies at metabolic steady state, offers a comprehensive platform to quantify plant central metabolism. In this chapter, we describe the application of INST-MFA to investigate metabolism in leaves...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28822070/chemical-shift-assignments-for-the-apo-form-of-the-catalytic-domain-the-linker-region-and-the-carbohydrate-binding-domain-of-the-cellulose-active-lytic-polysaccharide-monooxygenase-sclpmo10c
#17
Gaston Courtade, Zarah Forsberg, Gustav Vaaje-Kolstad, Vincent G H Eijsink, Finn L Aachmann
The apo-form of the 21.4 kDa catalytic domain and the 10.7 kDa carbohydrate binding domain of the AA10 family lytic polysaccharide monooxygenase ScLPMO10C from Streptomyces coelicolor have been isotopically labeled and recombinantly expressed in Escherichia coli. In this paper, we report the (1)H, (13)C, and (15)N chemical shift assignments of each individual domain as well as an ensemble of the assignment for the full-length protein, including its approximately 30-amino acid long linker.
August 18, 2017: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/28822035/identification-and-functional-characterization-of-a-p-coumaroyl-coa-2-hydroxylase-involved-in-the-biosynthesis-of-coumarin-skeleton-from-peucedanum-praeruptorum-dunn
#18
Ruolan Yao, Yucheng Zhao, Tingting Liu, Chuanlong Huang, Sheng Xu, Ziwei Sui, Jun Luo, Lingyi Kong
A p-coumaroyl CoA 2'-hydroxylase responsible for the formation of coumarin lactone ring was identified from Peucedanum praeruptorum Dunn and functionally characterized in vitro. Coumarins are important plant secondary metabolites with a variety of biological activities. Ortho-hydroxylation of cinnamates leads to the formation of coumarin lactone ring and is generally thought to be a key step in coumarin biosynthesis. However, ortho-hydroxylases, especially p-coumaroyl CoA 2'-hydroxylase (C2'H) responsible for the biosynthesis of the most common coumarin skeleton, have received insufficient attention...
August 18, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28822029/gene-structure-and-comparative-and-phylogenetic-analyses-of-catla-catla-cyp1a-full-length-cdna-and-its-responsiveness-to-benzo-a-pyrene-and-copper-sulphate-at-early-developmental-stages
#19
Kavita Kumari, Gireesh-Babu Pathakota, Shivendra Kumar, Gopal Krishna
In the present study, full-length CYP1A cDNA from Catla catla (Catla) has been identified, and its real-time quantitative reverse transcription PCR (qRT-PCR) expression has been evaluated in different tissues, developmental stages (0, 3, 6, 12 and 24 h and 5, 7 and 9 days post-fertilization) and copper sulphate and benzo(a)pyrene (BaP)-treated 5-day post-fertilization (dpf) larvae (6 to 6.5 mm). Various structural, comparative and phylogenetic analyses of the deduced amino acid sequence revealed that the identified gene of Catla belongs to the CYP1A1 subfamily...
August 19, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28821882/identification-of-a-short-sequence-in-the-hcmv-terminase-pul56-essential-for-interaction-with-pul89-subunit
#20
G Ligat, C Jacquet, S Chou, A Couvreux, S Alain, S Hantz
The human cytomegalovirus (HCMV) terminase complex consists of several components acting together to cleave viral DNA into unit length genomes and translocate them into capsids, a critical process in the production of infectious virions subsequent to DNA replication. Previous studies suggest that the carboxyl-terminal portion of the pUL56 subunit interacts with the pUL89 subunit. However, the specific interacting residues of pUL56 remain unknown. We identified a conserved sequence in the C-terminal moiety of pUL56 (671WMVVKYMGFF680)...
August 18, 2017: Scientific Reports
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