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https://www.readbyqxmd.com/read/28443083/intracellular-localization-interactions-and-functions-of-capsicum-chlorosis-virus-proteins
#1
Shirani M K Widana Gamage, Ralf G Dietzgen
Tospoviruses are among the most devastating viruses of horticultural and field crops. Capsicum chlorosis virus (CaCV) has emerged as an important pathogen of capsicum and tomato in Australia and South-east Asia. Present knowledge about CaCV protein functions in host cells is lacking. We determined intracellular localization and interactions of CaCV proteins by live plant cell imaging to gain insight into the associations of viral proteins during infection. Proteins were transiently expressed as fusions to autofluorescent proteins in leaf epidermal cells of Nicotiana benthamiana and capsicum...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28437434/comparisons-of-gnrh-antagonist-protocol-versus-gnrh-agonist-long-protocol-in-patients-with-normal-ovarian-reserve-a-systematic-review-and-meta-analysis
#2
Ruolin Wang, Shouren Lin, Yong Wang, Weiping Qian, Liang Zhou
OBJECTIVE: To evaluate the effectiveness and safety of gonadotropin-releasing hormone antagonist (GnRH-ant) protocol and gonadotropin-releasing hormone agonist (GnRH-a) long protocol in patients with normal ovarian reserve. METHODS: We searched the PubMed (1992-2016), Cochrane Library (1999-2016), Web of Science (1950-2016), Chinese Biomedical Database (CBM, 1979-2016), and China National Knowledge Infrastructure (CNKI, 1994-2016). Any randomized controlled trials (RCTs) that compared GnRH-ant protocol and GnRH-a long protocol in patients with normal ovarian reserve were included, and data were extracted independently by two reviewers...
2017: PloS One
https://www.readbyqxmd.com/read/28433053/the-envelope-proteins-of-the-bunyavirales
#3
Pablo Guardado-Calvo, Félix A Rey
The Bunyavirales Order encompasses nine families of enveloped viruses containing a single-stranded negative-sense RNA genome divided into three segments. The small (S) and large (L) segments encode proteins participating in genome replication in the infected cell cytoplasm. The middle (M) segment encodes the viral glycoproteins Gn and Gc, which are derived from a precursor polyprotein by host cell proteases. Entry studies are available only for a few viruses in the Order, and in each case they were shown to enter cells via receptor-mediated endocytosis...
2017: Advances in Virus Research
https://www.readbyqxmd.com/read/28422981/qtl-analysis-and-dissection-of-panicle-components-in-rice-using-advanced-backcross-populations-derived-from-oryza-sativa-cultivars-hr1128-and-nipponbare
#4
Zhizhong Sun, Xiaoling Yin, Jia Ding, Dong Yu, Miao Hu, Xuewu Sun, Yanning Tan, Xiabing Sheng, Ling Liu, Yi Mo, Ning Ouyang, Beibei Jiang, Guilong Yuan, Meijuan Duan, Dingyang Yuan, Jun Fang
Panicle traits are among the most important agronomic characters which directly relate to yield in rice. Grain number (GN), panicle length (PL), primary branch number (PBN), and secondary branch number (SBN) are the major components of rice panicle structure, and are all controlled by quantitative trait loci (QTLs). In our research, four advanced backcross overlapping populations (BIL152, BIL196a, BIL196b, and BIL196b-156) carrying introgressed segments from chromosome 6 were derived from an indica/japonica cross that used the super-hybrid rice restorer line HR1128 and the international sequenced japonica cultivar 'Nipponbare' as the donor and recurrent parents, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28422380/comparing-the-long-acting-and-short-acting-forms-of-gonadotropin-releasing-hormone-agonists-in-the-long-protocol-of-ivf-icsi-cycles-a-retrospective-study
#5
Liyan Duan, Shihua Bao, Kunming Li, Xiaoming Teng, Ling Hong, Xiaoyu Zhao
AIM: This study aimed to compare the efficacy of long- and short-acting gonadotropin-releasing hormone agonist on clinical outcomes of in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) long protocol cycles. METHODS: In this retrospective study, 478 patients were enrolled from October 2012 to November 2014. The pituitary downregulation result, serum hormone levels, gonadotropin (Gn) dose during controlled ovarian hyperstimulation, and outcome of IVF/ICSI-embryo transfer were compared between the two groups...
April 19, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28421524/distance-dependent-varieties-of-microbial-community-structure-and-metabolic-functions-in-the-rhizosphere-of-sedum-alfredii-hance-during-phytoextraction-of-a-cadmium-contaminated-soil
#6
Wenhao Yang, Taoxiang Zhang, Sen Lin, Wuzhong Ni
The recovery of microbial community and activities is crucial to the remediation of contaminated soils. Distance-dependent variations of microbial community composition and metabolic characteristics in the rhizospheric soil of hyperaccumulator during phytoextraction are poorly understood. A 12-month phytoextraction experiment with Sedum alfredii in a Cd-contaminated soil was conducted. A pre-stratified rhizobox was used for separating sub-layer rhizospheric (0-2, 2-4, 4-6, 6-8, 8-10 mm from the root mat)/bulk soils...
April 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28415902/metabolism-studies-on-hydroxygenkwanin-and-genkwanin-in-human-liver-microsomes-by-uhplc-q-tof-ms
#7
Lin Yuan, Caijuan Liang, Xinpeng Diao, Xiaoye Cheng, Man Liao, Lantong Zhang
Hydroxygenkwanin (HYGN) and genkwanin (GN) are major constituents of Genkwa Flos for the treatment of edema, ascites, cough, asthma, and cancer. This is a report about the investigation of the metabolic fate of HYGN and GN in human liver microsomes and the recombinant UGT enzymes by using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS). An on-line data acquisition method multiple mass defect filter (MMDF) combined with dynamic background subtraction (DBS) was developed to trace all probable metabolites...
April 18, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/28402128/effects-of-skeletally-anchored-class-ii-elastics-a-pilot-study-and-new-approach-for-treating-class-ii-malocclusion
#8
Selin Ozbilek, Ahmet Yalcin Gungor, Salih Celik
OBJECTIVE: To evaluate the skeletal, dentoalveolar, and soft tissue effects of skeletally anchored Class II elastics and compare them with a matched control group treated by a monobloc appliance for the correction of skeletal Class II malocclusion due to mandibular retrusion. MATERIALS AND METHODS: Twelve patients (6 girls, 6 boys) were randomly divided into two groups. In the elastics group, six patients (12.9 ± 1.5 years of age; 3 boys, 3 girls) were treated with skeletally anchored Class II elastics...
April 12, 2017: Angle Orthodontist
https://www.readbyqxmd.com/read/28400402/molecular-targeted-therapies-in-adrenal-pituitary-and-parathyroid-malignancies
#9
Anna Angelousi, Georgios K Dimitriadis, Gn Zografos, Svenja Nölting, Gregory A Kaltsas, Ashley B Grossman
Tumourigenesis is a relatively common event in endocrine tissues. Currently, specific guidelines have been developed for common malignant endocrine tumours which also incorporate advances in molecular targeted therapies (MTT), as in thyroid cancer and gastrointestinal neuroendocrine malignancies. However, there is little information regarding the role and efficacy of MTT in the relatively rare malignant endocrine tumours mainly involving the adrenal medulla, adrenal cortex, pituitary and parathyroid glands...
April 11, 2017: Endocrine-related Cancer
https://www.readbyqxmd.com/read/28398246/direct-electrochemical-detection-of-bisphenol-a-using-a-highly-conductive-graphite-nanoparticle-film-electrode
#10
Xinwei Dong, Xiaoli Qi, Na Liu, Yuesuo Yang, Yunxian Piao
We developed an accurate and sensitive sensor for electrochemical detection of bisphenol A (BPA) with a high-conductivity graphite nanoparticle (GN) film electrode. The GNs consisted of several stacked graphene sheets and showed a homogenous spherical shape, high conductivity, large surface area and good adsorption properties to BPA. The constructed GN film electrode exhibited improved amperometric current responses such as decreased impedance and lowered BPA oxidation potential compared with those of a pristine electrode, and also possessed a large surface area to allow fast electron transfer and BPA accumulation...
April 11, 2017: Sensors
https://www.readbyqxmd.com/read/28391345/new-approaches-to-trials-in-glomerulonephritis
#11
Jonathan C Craig, Allison Tong, Giovanni F M Strippoli
Randomized controlled trials are required to reliably identify interventions to improve the outcomes for people with glomerulonephritis (GN). Unfortunately, although easier, observational studies are inherently unreliable even though the findings of both study designs agree most of the time. Currently there are ∼790 trials in GN, but suboptimal design and reporting, together with small sample sizes, mean that they may not be reliable for decision making. If the history is somewhat bleak, the future looks bright, with recent initiatives to improve the quality, size and relevance of clinical trials in nephrology, including greater patient engagement, trial networks, core outcome sets, registry-based trials and adaptive designs...
January 1, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28391340/targeting-the-tyrosine-kinase-signalling-pathways-for-treatment-of-immune-mediated-glomerulonephritis-from-bench-to-bedside-and-beyond
#12
Terry King-Wing Ma, Stephen P McAdoo, Frederick Wai Keung Tam
Glomerulonephritis (GN) affects patients of all ages and is an important cause of morbidity and mortality. Non-selective immunosuppressive drugs have been used in immune-mediated GN but often result in systemic side effects and occasionally fatal infective complications. There is increasing evidence from both preclinical and clinical studies that abnormal activation of receptor and non-receptor tyrosine kinase signalling pathways are implicated in the pathogenesis of immune-mediated GN. Activation of spleen tyrosine kinase (SYK), Bruton's tyrosine kinase (BTK), platelet-derived growth factor receptor (PDGFR), epidermal growth factor receptor (EGFR) and discoidin domain receptor 1 (DDR1) have been demonstrated in anti-GBM disease...
January 1, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28391334/complement-activation-in-pauci-immune-necrotizing-and-crescentic-glomerulonephritis-results-of-a-proteomic-analysis
#13
Sanjeev Sethi, Ladan Zand, An S De Vriese, Ulrich Specks, Julie A Vrana, Siddak Kanwar, Paul Kurtin, Jason D Theis, Andrea Angioi, Lynn Cornell, Fernando C Fervenza
Background: Complement activation plays an important role in the pathophysiology of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), although it remains unclear which pathway is activated. Whether pauci-immune necrotizing crescentic glomerulonephritis (pauci-immune GN) with negative ANCA serology is part of the spectrum of AAV or a different disease entity is essentially unknown. Methods: We used proteomic analysis to delineate the complement profile in a series of 13 kidney biopsies of patients with pauci-immune GN, with either proteinase 3 (PR3) (five patients) or myeloperoxidase (MPO) antibodies (four patients) or with consistently negative ANCA serology (four patients)...
January 1, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28367605/prediction-of-class-ii-improvement-after-rapid-maxillary-expansion-in-early-mixed-dentition
#14
Alberto Caprioglio, Chiara Bergamini, Lorenzo Franchi, Nicolò Vercellini, Piero Antonio Zecca, Riccardo Nucera, Rosamaria Fastuca
BACKGROUND: The aim of this study is to identify cephalometric pretreatment parameters for prediction of Class II improvement induced by rapid maxillary expansion. METHODS: Lateral cephalograms of 30 patients (mean age 8.3 ± 1.6 years old) showing Class II molar relationship and undergone to rapid maxillary expansion on the upper deciduous molars were traced before treatment, and molar relation changes were evaluated on dental casts before and after treatment...
December 2017: Progress in Orthodontics
https://www.readbyqxmd.com/read/28361602/sex-differences-in-pharyngeal-airway-morphology-in-adults-with-skeletal-class-iii-malocclusion
#15
Maria Fernanda Rabelo Bozzini, José Valladares-Neto, João Batista de Paiva, José Rino-Neto
OBJECTIVES: To compare the pharyngeal airway dimensions in adults with skeletal Class III malocclusion and well-balanced faces, to ascertain whether sex can influence such measurements and to correlate skeletal pattern with pharyngeal dimensions. METHODOLOGY: Fifty adults were recruited and assigned to two groups: skeletal Class III (n = 25; 12 males and 13 females, mean age = 26.1 years), and skeletal Class I (n = 25; 11 males and 14 females, mean age = 26...
March 31, 2017: Cranio: the Journal of Craniomandibular Practice
https://www.readbyqxmd.com/read/28357988/isolation-of-lacinutrix-venerupis-strains-associated-with-disease-outbreaks-in-sea-bream-sparus-aurata-and-european-sea-bass-dicentrarchus-labrax
#16
Jose R López, Rafael Alcantara, Laura Lorenzo, J I Navas
Four Gram-negative bacterial isolates were recovered from 2 disease outbreaks that occurred in 2013 affecting European sea bass Dicentrarchus labrax fry and sea bream Sparus aurata adults. Main symptoms were erratic swimming, eroded fins and, in the sea bream outbreak, haemorrhages on the body surface; bacteria were always recovered from internal organs, almost in pure culture. On the basis of phenotypic characterization and 16S rRNA gene sequence analysis, the isolates were identified as Lacinutrix venerupis, a bacterium not previously reported as a fish pathogen...
March 30, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28356674/fibrillary-glomerulonephritis-presenting-as-crescentic-glomerulonephritis
#17
H H Shah, J Thakkar, J M Pullman, A T Mathew
Fibrillary glomerulonephritis (FGN) is a rare primary glomerular disease that commonly presents clinically with hypertension, proteinuria, microscopic hematuria, and varying degree of renal insufficiency. Histologically, FGN can present with different patterns of glomerular injury, more commonly mesangioproliferative, membranoproliferative, and membranous nephropathy. While crescent formation has been described in some kidney biopsy series of FGN, crescentic glomerulonephritis pattern of glomerular injury has been rarely described...
March 2017: Indian Journal of Nephrology
https://www.readbyqxmd.com/read/28352022/analysis-of-renal-diseases-detected-in-renal-biopsies-of-adult-patients-a-single-center-experience
#18
Salman Imtiaz, Murtaza F Drohlia, Kiran Nasir, Beena Salman, Aasim Ahmad
Renal biopsy is crucial while evaluating for the diagnosis of glomerular, vascular, tubulointerstitial, and genetic diseases. It gives vital information which helps in estimating the disease prognosis, progression, and management. This is the retrospective analysis of all adult patients aged above 18 years, who underwent percutaneous renal biopsy at The Kidney Center Post Graduate Training Institute, Karachi, over a duration of 18 years, i.e., January 1, 1996, to December 2013. Renal graft biopsies and those which were inadequate were excluded from analysis...
March 2017: Saudi Journal of Kidney Diseases and Transplantation
https://www.readbyqxmd.com/read/28345137/a-comparative-analysis-of-phosphoproteome-in-ovine-muscle-at-early-postmortem-in-relationship-to-tenderness-%C3%A2
#19
Xin Li, Lijuan Chen, Fan He, Meng Li, Qingwu Shen, Dequan Zhang
BACKGROUND: Tenderness is considered to be the most important quality characteristic of meat as it is the main cause of unacceptability of meat. Post-translational modification regulates protein functions that involve in postmortem changes in muscle and meat quality formation. Specifically, phosphorylation was proved to regulate postmortem glycolytic rates and meat tenderization. However, the relationship between protein phosphorylation and meat tenderness remains unclear. This study examined the phosphoproteomes found in ovine muscle with different degrees of tenderness over time (at 0...
March 26, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28340816/relevance-of-flow-cytometric-auto-crossmatch-to-the-post-transplant-course-of-kidney-transplant-recipients
#20
E Demir, O Yeğit, A Erol, S U Akgül, B Çalışkan, A Bayraktar, Y Çalışkan, A Türkmen, F O Savran, M S Sever
INTRODUCTION: The crossmatch test is essential prior to kidney transplantation (tx) to confirm compatibility between the donor and the recipient. However, its results can be misleading due to "undetectable antibodies" in the recipient's serum. To establish if undetectable autoantibodies are responsible for a positive result, an auto-crossmatch test can be performed. In this study, we aim to determine the long-term prognostic value of auto-flow cytometric auto-crossmatch (FCXM) test on kidney survival in kidney tx recipients...
April 2017: Transplantation Proceedings
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