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Platelet lysate

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https://www.readbyqxmd.com/read/29928900/characterization-and-comparison-of-human-limbal-explant-cultures-grown-under-defined-and-xeno-free-conditions
#1
Kristyna Brejchova, Peter Trosan, Pavel Studeny, Pavlina Skalicka, Tor Paaske Utheim, Jan Bednar, Katerina Jirsova
Human limbal epithelial cells (LECs) intended for treatment of limbal stem cell deficiency are commonly cultivated on a 3T3 feeder layer with complex culture medium supplemented with fetal bovine serum (FBS). However, FBS is a xenogeneic component containing poorly characterised constituents and exhibits quantitative and qualitative lot-to-lot variations. Human limbal explants were plated on untreated or fibrin coated plastic plates and cultured in two non-xenogeneic media (supplemented with either human serum or platelet lysate only)...
June 18, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29915318/low-affinity-nerve-growth-factor-receptor-cd271-heterogeneous-expression-in-adult-and-fetal-mesenchymal-stromal-cells
#2
Mario Barilani, Federica Banfi, Silvia Sironi, Enrico Ragni, Salomé Guillaumin, Francesca Polveraccio, Lorenzo Rosso, Monica Moro, Giuseppe Astori, Michela Pozzobon, Lorenza Lazzari
Human multipotent mesenchymal stromal cells (MSC) are isolated from a plethora of tissue sources for cell therapy purposes. In 2006, the International Society for Cellular Therapy (ISCT) published minimal guidelines to define MSC identity. Nevertheless, many independent studies demonstrated that cells meeting the ISCT criteria possessed heterogeneous phenotypes and functionalities, heavily influenced by culture conditions. In this study, human MSC derived from many adult (bone marrow and adipose tissue) or fetal (cord blood, Wharton's jelly, umbilical cord perivascular compartment and amniotic fluid) tissues were investigated...
June 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29904797/hyaluronic-acid-hydrogels-incorporating-platelet-lysate-enhance-human-pulp-cell-proliferation-and-differentiation
#3
Leopoldina D F Almeida, Pedro S Babo, Cristiana R Silva, Márcia T Rodrigues, Josimeri Hebling, Rui L Reis, Manuela E Gomes
The restoration of dentine-pulp complex remains a challenge for dentists; nonetheless, it has been poorly addressed. An ideal system should modulate the host response, as well as enable the recruitment, proliferation and differentiation of relevant progenitor cells. Herein was proposed a photocrosslinkable hydrogel system based on hyaluronic acid (HA) and platelet lysate (PL). PL is a cocktail of growth factors (GFs) and cytokines involved in wound healing orchestration, obtained by the cryogenic processing of platelet concentrates, and was expected to provide the HA hydrogels specific biochemical cues to enhance pulp cells' recruitment, proliferation and differentiation...
June 14, 2018: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29893509/characterization-of-induced-pluripotent-stem-cell-derived-megakaryocyte-lysates-for-potential-regenerative-applications
#4
Anja Baigger, Dorothee Eicke, Yuliia Yuzefovych, Denys Pogozhykh, Rainer Blasczyk, Constanca Figueiredo
Recently, platelet-derived growth factors present in lysates became an extreme interest in the field of regenerative medicine such as in wound healing and as substitutes to foetal bovine serum in xeno-free cell culture systems. However, the generation of such platelet lysates completely depends on the availability of platelet donors. In this study, the possibility to use in vitro-generated megakaryocytes derived from induced pluripotent stem cells (iPSCs) as a cell source for typical platelet growth factors was investigated...
June 12, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29876178/a-novel-porcine-model-for-future-studies-of-cell-enriched-fat-grafting
#5
Bo S Rasmussen, Celine L Sørensen, Peter V Vester-Glowinski, Mikkel Herly, Sorel Kurbegovic, Mathias Ørholt, Jesper D Svalgaard, Stig-Frederik T Kølle, Annemarie T Kristensen, Maj-Lis M Talman, Krzysztof T Drzewiecki, Anne Fischer-Nielsen
Background: Cell-enriched fat grafting has shown promising results for improving graft survival, although many questions remain unanswered. A large animal model is crucial for bridging the gap between rodent studies and human trials. We present a step-by-step approach in using the Göttingen minipig as a model for future studies of cell-enriched large volume fat grafting. Methods: Fat grafting was performed as bolus injections and structural fat grafting. Graft retention was assessed by magnetic resonance imaging after 120 days...
April 2018: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/29868646/extracellular-matrix-protein-production-in-human-adipose-derived-mesenchymal-stem-cells-on-three-dimensional-polycaprolactone-pcl-scaffolds-responds-to-gdf5-or-fgf2
#6
Yan Su, Janet M Denbeigh, Emily T Camilleri, Scott M Riester, Joshua A Parry, Eric R Wagner, Michael J Yaszemski, Allan B Dietz, Simon M Cool, Andre J van Wijnen, Sanjeev Kakar
Purpose: The poor healing potential of intra-articular ligament injuries drives a need for the development of novel, viable 'neo-ligament' alternatives. Ex vivo approaches combining stem cell engineering, 3-dimensional biocompatible scaffold design and enhancement of biological and biomechanical functionality via the introduction of key growth factors and morphogens, represent a promising solution to ligament regeneration. Methods: We investigated growth, differentiation and extracellular matrix (ECM) protein production of human adipose-derived mesenchymal stem/stromal cells (MSCs), cultured in 5% human platelet lysate (PL) and seeded on three-dimensional polycaprolactone (PCL) scaffolds, in response to the connective-tissue related ligands fibroblast growth factor 2 (basic) (FGF2) and growth and differentiation factor-5 (GDF5)...
March 2018: Gene Reports
https://www.readbyqxmd.com/read/29757934/comparison-of-synthetic-media-designed-for-expansion-of-adipose-derived-mesenchymal-stromal-cells
#7
Michelle Lensch, Angela Muise, Lisa White, Michael Badowski, David Harris
Mesenchymal stromal cells (MSCs) are multipotent cells that can differentiate into various cell types, such as osteoblasts, myocytes, and adipocytes. This characteristic makes the cells a useful tool in developing new therapies for a number of common maladies and diseases. The utilization of animal-derived growth serum, such as fetal bovine serum (FBS), for the expansion of MSCs has traditionally been used for cell culture. However, in clinical applications, animal-derived products present limitations and safety concerns for the recipient, as exposure to animal (xeno-) antigens and infectious agents is possible...
May 14, 2018: Biomedicines
https://www.readbyqxmd.com/read/29756266/the-analysis-of-false-prolongation-of-the-activated-partial-thromboplastin-time-activator-silica-interference-of-c-reactive-protein
#8
Jie Liu, Fanfan Li, Kuangyi Shu, Tao Chen, Xiaoou Wang, Yaoqi Xie, Shanshan Li, Zhaohua Zhang, Susu Jin, Minghua Jiang
BACKGROUND: To investigate the effect of C-reactive protein on the activated partial thromboplastin time (APTT) (different activators) in different detecting systems. METHODS: The C-reactive protein and coagulation test of 112 patients with the infectious disease were determined by automation protein analyzer IMMAG 800 and automation coagulation analyzer STA-R Evolution, respectively. The pooled plasma APTT with different concentrations of C-reactive protein was measured by different detecting system: STA-R Evolution (activator: silica, kaolin), Sysmex CS-2000i (activator: ellagic acid), and ACL TOP 700 (activator: colloidal silica)...
May 13, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29722764/platelet-rich-plasma-and-mesenchymal-stem-cells-exciting-but-%C3%A2-are-we-there-yet
#9
Isabel Andia, Jose I Martin, Nicola Maffulli
Joint conditions incapacitate free movement driving to a sedentary lifestyle, a major risk factor for chronic diseases. Regenerative procedures, involving the use of mesenchymal stem/stromal cells along with platelet-rich plasma (PRP), can help patients with these conditions. We describe the main characteristics of cellular products (bone marrow concentrate, stromal vascular fraction of adipose tissue, and mesenchymal stem/stromal cells derived from these tissues), and the potential benefits of combination with PRP in 3 scenarios: PRP lysates used during laboratory cell expansion; PRP to prime cellular products or the host tissue before cell implantation; PRP used as a vehicle for cell transplantation and to provide trophic signals...
June 2018: Sports Medicine and Arthroscopy Review
https://www.readbyqxmd.com/read/29720259/human-serum-and-platelet-lysate-are-appropriate-xeno-free-alternatives-for-clinical-grade-production-of-human-mustem-cell-batches
#10
Charlotte Saury, Aurélie Lardenois, Cindy Schleder, Isabelle Leroux, Blandine Lieubeau, Laurent David, Marine Charrier, Laëtitia Guével, Sabrina Viau, Bruno Delorme, Karl Rouger
BACKGROUND: Canine MuStem cells have demonstrated regenerative efficacy in a dog model of muscular dystrophy, and the recent characterization of human counterparts (hMuStem) has highlighted the therapeutic potential of this muscle-derived stem cell population. To date, these cells have only been generated in research-grade conditions. However, evaluation of the clinical efficacy of any such therapy will require the production of hMuStem cells in compliance with good manufacturing practices (GMPs)...
May 2, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29720245/human-platelet-lysate-in-mesenchymal-stromal-cell-expansion-according-to-a-gmp-grade-protocol-a-cell-factory-experience
#11
Valentina Becherucci, Luisa Piccini, Serena Casamassima, Silvia Bisin, Valentina Gori, Francesca Gentile, Riccardo Ceccantini, Elena De Rienzo, Barbara Bindi, Paola Pavan, Vanessa Cunial, Elisa Allegro, Stefano Ermini, Francesca Brugnolo, Giuseppe Astori, Franco Bambi
BACKGROUND: The use of platelet lysate (PL) for the ex-vivo expansion of mesenchymal stromal/stem cells (MSCs) was initially proposed by Doucet et al. in 2005, as an alternative to animal serum. Moreover, regulatory authorities discourage the use of fetal bovine serum (FBS) or other animal derivatives, to avoid risk of zoonoses and xenogeneic immune reactions. Even if many studies investigated PL composition, there still are some open issues related to its use in ex-vivo MSC expansion, especially according to good manufacturing practice (GMP) grade protocols...
May 2, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29714398/impact-of-human-platelet-lysate-on-the-expansion-and-chondrogenic-capacity-of-cultured-human-chondrocytes-for-cartilage-cell-therapy
#12
J G Sykes, J H Kuiper, J B Richardson, S Roberts, K T Wright, N J Kuiper
High hopes have been pinned on regenerative medicine strategies in order to prevent the progression of cartilage damage to osteoarthritis, particularly by autologous chondrocyte implantation (ACI). The loss of chondrocyte phenotype during in vitro monolayer expansion, a necessary step to obtain sufficient cell numbers, may be a key limitation in ACI. In this study, it was determined whether a shorter monolayer expansion approach could improve chondrogenic differentiation. The effects of two supplement types, foetal bovine serum (FBS) and Stemulate™ (a commercial source of human platelet lysate), on the expansion and re-differentiation potential of human chondrocytes, isolated from five individuals, were compared...
May 1, 2018: European Cells & Materials
https://www.readbyqxmd.com/read/29691826/supercritical-fluid-technology-as-a-tool-to-prepare-gradient-multifunctional-architectures-towards-regeneration-of-osteochondral-injuries
#13
Ana Rita C Duarte, Vitor E Santo, Manuela E Gomes, Rui L Reis
Platelet lysates (PLs) are a natural source of growth factors (GFs) known for its stimulatory role on stem cells which can be obtained after activation of platelets from blood plasma. The possibility to use PLs as growth factor source for tissue healing and regeneration has been pursued following different strategies. Platelet lysates are an enriched pool of growth factors which can be used as either a GFs source or as a three-dimensional (3D) hydrogel. However, most of current PLs-based hydrogels lack stability, exhibiting significant shrinking behavior...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29673841/electrospun-silk-fibroin-fibers-for-storage-and-controlled-release-of-human-platelet-lysate
#14
Cataldo Pignatelli, Giovanni Perotto, Marta Nardini, Ranieri Cancedda, Maddalena Mastrogiacomo, Athanassia Athanassiou
Human platelet lysate (hPL) is a pool of growth factors and cytokines able to induce regeneration of different tissues. Despite its good potentiality as therapeutic tool for regenerative medicine applications, hPL has been only moderately exploited in this field. A more widespread adoption has been limited because of its rapid degradation at room temperature that decreases its functionality. Another limiting factor for its extensive use is the difficulty of handling the hPL gels. In this work, silk fibroin-based patches were developed to address several points: improving the handling of hPL, enabling their delivery in a controlled manner and facilitating their storage by creating a device ready to use with expanded shelf life...
June 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29661028/development-of-large-scale-manufacturing-of-adipose-derived-stromal-cells-for-clinical-applications-using-bioreactors-and-human-platelet-lysate
#15
Mandana Haack-Sørensen, Morten Juhl, Bjarke Follin, Rebekka Harary Søndergaard, Maria Kirchhoff, Jens Kastrup, Annette Ekblond
In vitro expanded adipose-derived stromal cells (ASCs) are a useful resource for tissue regeneration. Translation of small-scale autologous cell production into a large-scale, allogeneic production process for clinical applications necessitates well-chosen raw materials and cell culture platform. We compare the use of clinical-grade human platelet lysate (hPL) and fetal bovine serum (FBS) as growth supplements for ASC expansion in the automated, closed hollow fibre quantum cell expansion system (bioreactor)...
April 17, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/29581732/isolation-culture-and-induced-differentiation-of-rabbit-mesenchymal-stem-cells-into-osteoblasts
#16
Hao Liu, Li-Kun Wei, Xiao-Fei Jian, Jie Huang, Hui Zou, Shi-Zhan Zhang, Guang-Hua Yuan
Mesenchymal stem cells (MSCs) may be easily isolated from the bone marrow, and possess multi-lineage differentiation potential and various therapeutic applications. The differentiation of MSCs into osteoblasts is a complex process that is regulated by multiple internal and external factors. In the present study, the differentiation of MSCs isolated from rabbit bone marrow into osteoblasts using different osteoblast inductive media in the presence of dexamethasone, bone morphogenetic protein 2 (BMP-2), 1,25-dihydroxyvitamin D3, transforming growth factor β (TGFβ), platelet lysate and cyclooxygenase 2 (COX2), respectively...
April 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29577822/psychocardiological-disorder-and-brain-serotonin-after-comorbid-myocardial-infarction-and-depression-an-experimental-study
#17
Li-Jun Zhang, Mei-Yan Liu, Radhika Rastogi, Jessie N Ding
Objectives We investigated whether trimetazidine pretreatment can regulate central and peripheral serotonin (5-HT) in rats of myocardial infarction (MI) combined with depression. Methods Forty rats were randomly assigned to a sham operation group (n = 10) and a disease model group (n = 30). The sham operation group was pretreated with normal saline for 4 weeks. The disease model group was randomly assigned further into a negative control subgroup, a positive control subgroup, and a treatment subgroup - the groups received saline, sertraline, and trimetazidine pretreatment, respectively, for 4 weeks, then the rats were subjected to MI combined with depression...
March 26, 2018: Neurological Research
https://www.readbyqxmd.com/read/29574078/a-novel-dressing-for-the-combined-delivery-of-platelet-lysate-and-vancomycin-hydrochloride-to-chronic-skin-ulcers-hyaluronic-acid-particles-in-alginate-matrices
#18
S Rossi, M Mori, B Vigani, M C Bonferoni, G Sandri, F Riva, C Caramella, F Ferrari
The aim of the present work was to develop a medication allowing for the combined delivery of platelet lysate (PL) and an anti-infective model drug, vancomycin hydrochloride (VCM), to chronic skin ulcers. A simple method was set up for the preparation of hyaluronic acid (HA) core-shell particles, loaded with PL and coated with calcium alginate, embedded in a VCM containing alginate matrix. Two different CaCl2 concentrations were investigated to allow for HA/PL core-shell particle formation. The resulting dressings were characterized for mechanical and hydration properties and tested in vitro (on fibroblasts) and ex-vivo (on skin biopsies) for biological activity...
June 15, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29568314/platelet-lysate-induces-chondrogenic-differentiation-of-umbilical-cord-derived-mesenchymal-stem-cells
#19
Ghmkin Hassan, Mohammad Bahjat, Issam Kasem, Chadi Soukkarieh, Majd Aljamali
Purpose: Articular cartilage has a poor capacity for self-repair, and thus still presents a major challenge in orthopedics. Mesenchymal stem cells (MSCs) are multipotent stem cells with the potential to differentiate into chondrocytes in the presence of transforming growth factor beta (TGF-β). Platelet lysate (PL) contains a relatively large number of growth factors, including TGF-β, and has been shown to ameliorate cartilage repair. Here, we investigated the ability of PL to direct chondrogenic differentiation of MSCs along with other standard differentiation components in a pellet culture system...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29566772/platelet-lysate-as-a-novel-serum-free-media-supplement-for-the-culture-of-equine-bone-marrow-derived-mesenchymal-stem-cells
#20
Maria C Naskou, Scarlett M Sumner, Anna Chocallo, Hannah Kemelmakher, Merrilee Thoresen, Ian Copland, Jacques Galipeau, John F Peroni
BACKGROUND: Mesenchymal stem cells (MSCs) produced for clinical purposes rely on culture media containing fetal bovine serum (FBS) which is xenogeneic and has the potential to significantly alter the MSC phenotype, rendering these cells immunogenic. As a result of bovine-derived exogenous proteins expressed on the cell surface, MSCs may be recognized by the host immune system as non-self and be rejected. Platelet lysate (PL) may obviate some of these concerns and shows promising results in human medicine as a possible alternative to FBS...
March 22, 2018: Stem Cell Research & Therapy
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