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Platelet lysate

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https://www.readbyqxmd.com/read/28024508/-reconstruction-of-humanized-bone-marrow-niche-in-immunodeficiency-mouse
#1
Huan Chen, Zheng Tian, Bin Li, Hai-Yan Xing, Huan Li, Ke-Jing Tang, Min Wang, Qing Rao
OBJECTIVE: To reconstruct a human bone marrow niche in immunodeficiency mouse (NOD/SCID) so as to provide a model for observing the effect of abnormal BM niche on the occurence and development of leukemia. METHODS: Human platelet lysate(HPL) was obtained by repeated freezing and thawing of concentrated platelet. Bone marrow-derived mesenchymal stem cells were cultured in α-minimal essential medium (α-MEM) containing 10% HPL or 10% FBS. The morphology, cell phenotype, multilineage differentiation potential in vitro and proliferation capacity between the mesenchymal stem cells cultured with HPL or FBS were compared...
December 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28013442/dmso-free-cryopreservation-of-adipose-derived-mesenchymal-stromal-cells-expansion-medium-affects-post-thaw-survival
#2
Olena Rogulska, Yuri Petrenko, Alexander Petrenko
Off-the-shelf availability of human adipose-derived mesenchymal stromal cells (ASCs) for regenerative medicine application requires the development of nontoxic, safe, and efficient protocols for cryopreservation. Favorably, such cell processing protocols should not contain xenogeneic or toxic components, such as fetal bovine serum (FS) and dimethyl sulfoxide (DMSO). The objective of the study was to assess the sensitivity of ASCs to DMSO-free cryopreservation protocol depending on their expansion conditions: conventional, based on the application of FS or xeno-free, using PL as a medium supplement...
December 24, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27968851/development-of-the-novel-antiplatelet-agent-adtm-originating-from-traditional-chinese-medicine-a-chemical-proteomic-analysis-and-in-vivo-assessment-of-efficacy-in-an-animal-model
#3
Guozhen Cui, Angela Wa Ho, Simon Ming Yuen Lee, Yuqiang Wang, Maggie Pui Man Hoi
BACKGROUND: An ageing population and lifestyle changes have led to increased cardiovascular disease-related morbidity and mortality in China, where an estimated 230 million people have cardiovascular disease. Long-term antiplatelet therapy with aspirin and clopidogrel are used for treatment and post-stenting primary prevention of acute coronary syndrome, but the risk of bleeding complications is high. This study aimed to identify the target of the newly developed antiplatelet agent ADTM, originating from traditional Chinese herbs...
October 2016: Lancet
https://www.readbyqxmd.com/read/27958222/effects-of-ginseng-fruit-saponins-on-serotonin-system-in-sprague-dawley-rats-with-myocardial-infarction-depression-and-myocardial-infarction-complicated-with-depression
#4
Dong-Fang He, Yan-Ping Ren, Mei-Yan Liu
BACKGROUND: Our previous studies have demonstrated that the levels of 5-hydroxytryptamine (5-HT) and 5-HT 2A receptor (5-HT2AR) in serum and platelet were associated with depression and myocardial infarction (MI), and pretreatment with ginseng fruit saponins (GFS) before MI and depression had an effect on the 5-HT system. In this study, the effects of GFS on the 5-HT system in the Sprague-Dawley (SD) rats with MI, depression, and MI + depression were evaluated. METHODS: A total of eighty SD rats were allocated to four groups: MI, depression, MI + depression, and control groups (n = 20 in each group)...
2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27943660/clinical-scale-expansion-of-adipose-derived-stromal-cells-starting-from-stromal-vascular-fraction-in-a-single-use-bioreactor-proof-of-concept-for-autologous-applications
#5
Mélanie Gadelorge, Marion Bourdens, Nicolas Espagnolle, Clémence Bardiaux, Julie Murrell, Lenaig Savary, Sylvain Ribaud, Benoît Chaput, Luc Sensebe
Adipose derived stromal cells (ASC) are adult multipotent cells increasingly used for cell therapy due to their differentiation potential, their paracrine effect and their convenience. ASC are currently selected from stromal vascular fraction (SVF) of adipose tissue and expanded in 2D flasks following Good Manufacturing Practices. This process is limited in surface area, labor-intensive and expensive, especially for autologous applications requiring selection and expansion steps for every patient. Closed and automated bioreactors offer an alternative for scalable and cost-effective production of ASCs...
December 11, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27943621/the-protective-effect-of-human-platelet-lysate-in-models-of-neurodegenerative-disease-involvement-of-the-akt-and-mek-pathways
#6
Flore Gouel, Bruce Do Van, Ming-Li Chou, Aurélie Jonneaux, Caroline Moreau, Régis Bordet, Thierry Burnouf, Jean-Christophe Devedjian, David Devos
Neurodegenerative diseases have huge economic and societal impacts, and place an immense emotional burden on patients and caregivers. Given that platelets have an essential physiological role in wound healing and tissue repair, human platelet lysates (HPLs) are being developed as a novel, effective biotherapy for neurodegenerative diseases. HPLs constitute abundant, readily accessible sources of physiological mixtures of many growth factors (GFs), with demonstrable effects on neuron survival and thus the development, maintenance, function and plasticity of the vertebrate nervous system...
December 12, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27941770/polyvalent-immunoglobulins-platelet-lysate-and-lenalidomide-cocktail-for-polyfunctional-nk-cells-expansion-for-multiple-myeloma
#7
H Trébéden-Negre, V Vieillard, M Rosenzwajg, L Garderet, M Cherai, S Nguyen-Quoc, M L Tanguy, F Norol
No abstract text is available yet for this article.
December 12, 2016: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/27939374/mesenchymal-stromal-cell-secreted-chemerin-is-a-novel-immunomodulatory-molecule-driving-the-migration-of-chemr23-expressing-cells
#8
Paola Vinci, Antonio Bastone, Silvia Schiarea, Claudia Cappuzzello, Annalisa Del Prete, Erica Dander, Andrea Biondi, Giovanna D'Amico
BACKGROUND: Mesenchymal stromal cells (MSCs) are multipotent cells characterized by broad immunomodulatory properties exploited for the treatment of inflammatory disorders. However, the efficacy of MSC-based therapy is highly variable and tightly linked to MSC culture conditions and treatment schedule. Thus, the identification of novel key molecules regulating MSC immunomodulatory activities in vivo might constitute a crucial step toward the optimization of currently available clinical protocols...
February 2017: Cytotherapy
https://www.readbyqxmd.com/read/27933268/autologous-platelet-lysates-local-injections-for-treatment-of-tibia-non-union-with-breakage-of-the-nickelclad-a-case-report
#9
Hong-Jiang Jiang, Xun-Xiang Tan, Hai-Yang Ju, Jin-Ping Su, Wei Yan, Xiu-Gang Song, Li-Wu Qin, Chang-Jun Ju, Ling-Shuang Wang, De-Bao Zou
BACKGROUND: Nonunions of the tibia represent challenging orthopedic problems, which require the surgeon to analyze numerous factors and choose an appropriate treatment. This article presents a case report of tibia and fibula fracture patient who failed the internal fixation surgery and successfully recovered after one course of percutaneous autologous platelet lysates injection. CASE DESCRIPTION: The patient received an internal nickelclad breakage at 9 months post-surgery but reluctant to accept a second surgery, then autologous platelet lysates (APL) injection which is a less invasive method was recommended...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27890405/platelets-inhibit-migration-of-canine-osteosarcoma-cells
#10
S C Bulla, P R Badial, R C Silva, K Lunsford, C Bulla
The interaction between platelets and tumour cells is important for tumour growth and metastasis. Thrombocytopenia or antiplatelet treatment negatively impact on cancer metastasis, demonstrating potentially important roles for platelets in tumour progression. To our knowledge, there is no information regarding the role of platelets in cancer progression in dogs. This study was designed to test whether canine platelets affected the migratory behaviour of three canine osteosarcoma cell lines and to give insights of molecular mechanisms...
November 24, 2016: Journal of Comparative Pathology
https://www.readbyqxmd.com/read/27887865/overcoming-the-bottleneck-of-platelet-lysate-supply-in-large-scale-clinical-expansion-of-adipose-derived-stem-cells-a-comparison-of-fresh-versus-three-types-of-platelet-lysates-from-outdated-buffy-coat-derived-platelet-concentrates
#11
Peter V Glovinski, Mikkel Herly, Anders B Mathiasen, Jesper D Svalgaard, Rehannah Borup, Maj-Lis M Talman, Jens J Elberg, Stig-Frederik T Kølle, Krzysztof T Drzewiecki, Anne Fischer-Nielsen
BACKGROUND: Platelet lysates (PL) represent a promising replacement for xenogenic growth supplement for adipose-derived stem cell (ASC) expansions. However, fresh platelets from human blood donors are not clinically feasible for large-scale cell expansion based on their limited supply. Therefore, we tested PLs prepared via three methods from outdated buffy coat-derived platelet concentrates (PCs) to establish an efficient and feasible expansion of ASCs for clinical use. METHODS: PLs were prepared by the freeze-thaw method from freshly drawn platelets or from outdated buffy coat-derived PCs stored in the platelet additive solution, InterSol...
February 2017: Cytotherapy
https://www.readbyqxmd.com/read/27882464/analysis-of-reparative-activity-of-platelet-lysate-effect-on-cell-monolayer-recovery-in-vitro-and-skin-wound-healing-in-vivo
#12
N S Sergeeva, Ya D Shanskii, I K Sviridova, P A Karalkin, V A Kirsanova, S A Akhmedova, A D Kaprin
Platelet lysate prepared from donor platelet concentrate and pooled according to a developed technique stimulates migration of multipotent mesenchymal stromal cells of the human adipose tissue and promotes healing of the monolayer defect in cultures of human fibroblasts and multipotent mesenchymal stromal cells in vitro in concentrations close those of fetal calf serum (5-10%). Lysate of platelets from platelet-rich rat blood plasma stimulated healing of the skin defect by promoting epithelialization and granulation tissue formation...
November 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27865782/application-of-nano-lc-maldi-tof-tof-ms-for-proteomic-analysis-of-microvesicles
#13
Joanna Kasprzyk, Ewa Stępień, Wojciech Piekoszewski
Activated platelets and platelet derived microvesicles (PMVs) emerged recently to be promising biomarkers. There is no universal procedure to carry out the proteomic analysis on microvesicles. In this study we proposed a nano-liquid chromatography (nano-LC) technique coupled off-line with a spectrometric measurement MALDI-TOF-MS/MS as a throughput and time-saving procedure. In this study we developed a simplified method to evaluate the protein composition of platelet organelles and PMVs. Platelet-rich plasma (PRP) was collected from healthy donors...
November 16, 2016: Clinical Biochemistry
https://www.readbyqxmd.com/read/27849108/influence-of-calcium-salts-and-bovine-thrombin-on-growth-factor-release-from-equine-platelet-rich-gel-supernatants
#14
Carlos E Giraldo, María E Álvarez, Jorge U Carmona
OBJECTIVE: To compare five activation methods in equine platelet-rich plasma (PRP) by determination of platelet-derived growth factor BB (PDGF-BB) and transforming growth factor beta 1 (TGF-β1) concentrations in platelet-rich gel (PRG) supernatants. METHODS: Platelet-rich plasma from 20 horses was activated by calcium chloride (CC), calcium gluconate (CG), bovine thrombin (BT), and their combinations, BTCC and BTCG. Both growth factor concentrations in PRG supernatants were measured by ELISA and compared with plasma and platelet lysates (PL) over time...
November 16, 2016: Veterinary and Comparative Orthopaedics and Traumatology: V.C.O.T
https://www.readbyqxmd.com/read/27848264/underlying-mechanism-and-specific-prevention-of-hemolysis-induced-platelet-activation
#15
Thomas Gremmel, Simone Fedrizzi, Günter Weigel, Beate Eichelberger, Simon Panzer
Thromboembolic complications significantly impair the outcome of hemolytic disorders. We hypothesized that red cell adenosine diphosphate (ADP) release results in significant platelet activation in hemolysis and that this prothrombotic state can be prevented by inhibition of the ADP P2Y12 receptor. In the current study, we therefore sought to investigate the mechanism and inhibition of hemolysis-induced platelet activation. The expression of activated integrin αIIbß3 was determined by flow cytometry, and platelet aggregation was assessed by multiple electrode platelet aggregometry...
November 16, 2016: Platelets
https://www.readbyqxmd.com/read/27837159/variability-in-platelet-derived-growth-factor-receptor-alpha-antibody-specificity-may-impact-clinical-utility-of-immunohistochemistry-assays
#16
Timothy R Holzer, Leslie O'Neill Reising, Kelly M Credille, Andrew E Schade, Gerard J Oakley
Aberrant regulation of the receptor tyrosine kinase platelet-derived growth factor alpha (PDGFRα) is implicated in several types of cancer. Inhibition of the PDGFRα pathway may be a beneficial therapy, and detection of PDGFRα in tumor biopsies may lead to insights about which patients respond to therapy. Exploratory or clinical biomarker use of PDGFRα IHC has been frequently reported, often with polyclonal antibody sc-338. An sc-338-based assay was systematically compared with anti-PDGFRα rabbit monoclonal antibody D13C6 using immunoblot profiling and IHC in formalin-fixed and paraffin-embedded human tumor cell lines...
December 2016: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/27798372/safety-studies-for-use-of-adipose-tissue-derived-mesenchymal-stromal-stem-cells-in-a-rabbit-model-for-osteoarthritis-to-support-a-phase-i-clinical-trial
#17
Scott M Riester, Janet M Denbeigh, Yang Lin, Dakota L Jones, Tristan de Mooij, Eric A Lewallen, Hai Nie, Christopher R Paradise, Darcie J Radel, Amel Dudakovic, Emily T Camilleri, Dirk R Larson, Wenchun Qu, Aaron J Krych, Matthew A Frick, Hee-Jeong Im, Allan B Dietz, Jay Smith, Andre J van Wijnen
: : Adipose-derived mesenchymal stem cells (AMSCs) offer potential as a therapeutic option for clinical applications in musculoskeletal regenerative medicine because of their immunomodulatory functions and capacity for trilineage differentiation. In preparation for a phase I clinical trial using AMSCs to treat patients with osteoarthritis, we carried out preclinical studies to assess the safety of human AMSCs within the intra-articular joint space. Culture-expanded human AMSCs grown in human platelet-lysate were delivered via intra-articular injections into normal healthy rabbit knees and knees at risk for the development of osteoarthritis after bilateral medial anterior hemimeniscectomy...
October 26, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27788223/humanizing-the-protease-activated-receptor-par-expression-profile-in-mouse-platelets-by-knocking-par1-into-the-par3-locus-reveals-par1-expression-is-not-tolerated-in-mouse-platelets
#18
Shauna L French, Antonia C Paramitha, Mitchell J Moon, Ross A Dickins, Justin R Hamilton
Anti-platelet drugs are the mainstay of pharmacotherapy for heart attack and stroke prevention, yet improvements are continually sought. Thrombin is the most potent activator of platelets and targeting platelet thrombin receptors (protease-activated receptors; PARs) is an emerging anti-thrombotic approach. Humans express two PARs on their platelets-PAR1 and PAR4. The first PAR1 antagonist was recently approved for clinical use and PAR4 antagonists are in early clinical development. However, pre-clinical studies examining platelet PAR function are challenging because the platelets of non-primates do not accurately reflect the PAR expression profile of human platelets...
2016: PloS One
https://www.readbyqxmd.com/read/27774106/highly-efficient-in-vitro-reparative-behaviour-of-dental-pulp-stem-cells-cultured-with-standardised-platelet-lysate-supplementation
#19
Pasquale Marrazzo, Francesco Paduano, Francesca Palmieri, Massimo Marrelli, Marco Tatullo
Dental pulp is an accessible source of multipotent mesenchymal stromal cells (MSCs). The perspective role of dental pulp stem cells (DPSCs) in regenerative medicine demands an in vitro expansion and in vivo delivery which must deal with the safety issues about animal serum, usually required in cell culture practice. Human platelet lysate (PL) contains autologous growth factors and has been considered as valuable alternative to fetal bovine serum (FBS) in cell cultures. The optimum concentration to be added of such supplement is highly dependent on its preparation whose variability limits comparability of results...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27771213/senescence-and-quiescence-in-adipose-derived-stromal-cells-effects-of-human-platelet-lysate-fetal-bovine-serum-and-hypoxia
#20
Rebekka Harary Søndergaard, Bjarke Follin, Lisbeth Drozd Lund, Morten Juhl, Annette Ekblond, Jens Kastrup, Mandana Haack-Sørensen
BACKGROUND AIMS: Adipose-derived stromal cells (ASCs) are attractive sources for cell-based therapies. The hypoxic niche of ASCs in vivo implies that cells will benefit from hypoxia during in vitro expansion. Human platelet lysate (hPL) enhances ASC proliferation rates, compared with fetal bovine serum (FBS) at normoxia. However, the low proliferation rates of FBS-expanded ASCs could be signs of senescence or quiescence. We aimed to determine the effects of hypoxia and hPL on the expansion of ASCs and whether FBS-expanded ASCs are senescent or quiescent...
January 2017: Cytotherapy
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