keyword
MENU ▼
Read by QxMD icon Read
search

Platelet lysate

keyword
https://www.readbyqxmd.com/read/29673841/electrospun-silk-fibroin-fibers-for-storage-and-controlled-release-of-human-platelet-lysate
#1
Cataldo Pignatelli, Giovanni Perotto, Marta Nardini, Ranieri Cancedda, Maddalena Mastrogiacomo, Athanassia Athanassiou
Human platelet lysate (hPL) is a pool of growth factors and cytokines able to induce regeneration of different tissues. Despite its good potentiality as therapeutic tool for regenerative medicine applications, hPL has been only moderately exploited in this field. A more widespread adoption has been limited because of its rapid degradation at room temperature that decreases its functionality. Another limiting factor for its extensive use is the difficulty of handling the hPL gels. In this work, silk fibroin-based patches were developed to address several points: improving the handling of hPL, enabling their delivery in a controlled manner and facilitating their storage by creating a device ready to use with expanded shelf life...
April 16, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29661028/development-of-large-scale-manufacturing-of-adipose-derived-stromal-cells-for-clinical-applications-using-bioreactors-and-human-platelet-lysate
#2
Mandana Haack-Sørensen, Morten Juhl, Bjarke Follin, Rebekka Harary Søndergaard, Maria Kirchhoff, Jens Kastrup, Annette Ekblond
In vitro expanded adipose-derived stromal cells (ASCs) are a useful resource for tissue regeneration. Translation of small-scale autologous cell production into a large-scale, allogeneic production process for clinical applications necessitates well-chosen raw materials and cell culture platform. We compare the use of clinical-grade human platelet lysate (hPL) and fetal bovine serum (FBS) as growth supplements for ASC expansion in the automated, closed hollow fibre quantum cell expansion system (bioreactor)...
April 17, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/29581732/isolation-culture-and-induced-differentiation-of-rabbit-mesenchymal-stem-cells-into-osteoblasts
#3
Hao Liu, Li-Kun Wei, Xiao-Fei Jian, Jie Huang, Hui Zou, Shi-Zhan Zhang, Guang-Hua Yuan
Mesenchymal stem cells (MSCs) may be easily isolated from the bone marrow, and possess multi-lineage differentiation potential and various therapeutic applications. The differentiation of MSCs into osteoblasts is a complex process that is regulated by multiple internal and external factors. In the present study, the differentiation of MSCs isolated from rabbit bone marrow into osteoblasts using different osteoblast inductive media in the presence of dexamethasone, bone morphogenetic protein 2 (BMP-2), 1,25-dihydroxyvitamin D3, transforming growth factor β (TGFβ), platelet lysate and cyclooxygenase 2 (COX2), respectively...
April 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29577822/psychocardiological-disorder-and-brain-serotonin-after-comorbid-myocardial-infarction-and-depression-an-experimental-study
#4
Li-Jun Zhang, Mei-Yan Liu, Radhika Rastogi, Jessie N Ding
Objectives We investigated whether trimetazidine pretreatment can regulate central and peripheral serotonin (5-HT) in rats of myocardial infarction (MI) combined with depression. Methods Forty rats were randomly assigned to a sham operation group (n = 10) and a disease model group (n = 30). The sham operation group was pretreated with normal saline for 4 weeks. The disease model group was randomly assigned further into a negative control subgroup, a positive control subgroup, and a treatment subgroup - the groups received saline, sertraline, and trimetazidine pretreatment, respectively, for 4 weeks, then the rats were subjected to MI combined with depression...
March 26, 2018: Neurological Research
https://www.readbyqxmd.com/read/29574078/a-novel-dressing-for-the-combined-delivery-of-platelet-lysate-and-vancomycin-hydrochloride-to-chronic-skin-ulcers-hyaluronic-acid-particles-in-alginate-matrices
#5
S Rossi, M Mori, B Vigani, M C Bonferoni, G Sandri, F Riva, C Caramella, F Ferrari
The aim of the present work was to develop a medication allowing for the combined delivery of platelet lysate (PL) and an anti-infective model drug, vancomycin hydrochloride (VCM), to chronic skin ulcers. A simple method was set up for the preparation of hyaluronic acid (HA) core-shell particles, loaded with PL and coated with calcium alginate, embedded in a VCM containing alginate matrix. Two different CaCl2 concentrations were investigated to allow for HA/PL core-shell particle formation. The resulting dressings were characterized for mechanical and hydration properties and tested in vitro (on fibroblasts) and ex-vivo (on skin biopsies) for biological activity...
March 21, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29568314/platelet-lysate-induces-chondrogenic-differentiation-of-umbilical-cord-derived-mesenchymal-stem-cells
#6
Ghmkin Hassan, Mohammad Bahjat, Issam Kasem, Chadi Soukkarieh, Majd Aljamali
Purpose: Articular cartilage has a poor capacity for self-repair, and thus still presents a major challenge in orthopedics. Mesenchymal stem cells (MSCs) are multipotent stem cells with the potential to differentiate into chondrocytes in the presence of transforming growth factor beta (TGF-β). Platelet lysate (PL) contains a relatively large number of growth factors, including TGF-β, and has been shown to ameliorate cartilage repair. Here, we investigated the ability of PL to direct chondrogenic differentiation of MSCs along with other standard differentiation components in a pellet culture system...
2018: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29566772/platelet-lysate-as-a-novel-serum-free-media-supplement-for-the-culture-of-equine-bone-marrow-derived-mesenchymal-stem-cells
#7
Maria C Naskou, Scarlett M Sumner, Anna Chocallo, Hannah Kemelmakher, Merrilee Thoresen, Ian Copland, Jacques Galipeau, John F Peroni
BACKGROUND: Mesenchymal stem cells (MSCs) produced for clinical purposes rely on culture media containing fetal bovine serum (FBS) which is xenogeneic and has the potential to significantly alter the MSC phenotype, rendering these cells immunogenic. As a result of bovine-derived exogenous proteins expressed on the cell surface, MSCs may be recognized by the host immune system as non-self and be rejected. Platelet lysate (PL) may obviate some of these concerns and shows promising results in human medicine as a possible alternative to FBS...
March 22, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29553865/comparison-of-human-platelet-lysate-alternatives-using-expired-and-freshly-isolated-platelet-concentrates-for-adipose-derived-stromal-cell-expansion
#8
Carla Dessels, Chrisna Durandt, Michael S Pepper
Pooled human platelet lysate (pHPL) has been used to expand adipose-derived stromal cells (ASCs) and can be formulated using fresh or expired buffy coats (BCs) which are then resuspended in either plasma or an additive solution. Not much is known about the effects that expired products and additive solutions have on ASC expansion, and the need for quality control and release criteria has been expressed. This pilot study compared proliferation, cell size, morphology and immunophenotype of ASCs expanded in the different pHPL alternatives versus foetal bovine serum (FBS)...
March 19, 2018: Platelets
https://www.readbyqxmd.com/read/29547658/the-corneal-epitheliotrophic-abilities-of-lyophilized-powder-form-human-platelet-lysates
#9
Lily Wei Chen, Chien-Jung Huang, Wen-Hui Tu, Chia-Ju Lu, Yi-Chen Sun, Szu-Yuan Lin, Wei-Li Chen
PURPOSE: To evaluate whether lyophilized human platelet lysate (HPL) powder can preserve the growth factor concentrations and epitheliotrophic properties of liquid HPL, and potentially be used as a clinically-friendly treatment option. METHODS: Two commercialized liquid HPLs, UltraGRO TM (Helios, Atlanta, GA) and PLTMax (Mill Creek, Rochester, MI), were obtained and converted to lyophilized powder. After redissolution, lyophilized powder HPLs were compared with liquid HPLs, as well as human peripheral serum (HPS) and fetal bovine serum (FBS) in liquid or redissolved lyophilized powder forms...
2018: PloS One
https://www.readbyqxmd.com/read/29536393/clinical-and-research-approaches-to-treat-non-union-fracture
#10
REVIEW
Claudia Schlundt, Christian H Bucher, Serafeim Tsitsilonis, Hanna Schell, Georg N Duda, Katharina Schmidt-Bleek
PURPOSE OF REVIEW: Impaired healing outcomes or even non-unions after bone injury are still a highly relevant problem in the daily clinical life. Especially within an aging population, the occurrence of bone fractures increases and thus novel treatment approaches to overcome compromised bone regeneration are needed. RECENT FINDINGS: The gold standard to treat delayed or non-healing bone injuries is still the use of autologous bone grafts to foster regeneration. Besides its successful treatment outcome, it also has disadvantages: a second surgery is needed in order to harvest the bone material and the material is highly limited...
March 13, 2018: Current Osteoporosis Reports
https://www.readbyqxmd.com/read/29531818/preserving-self-renewal-of-porcine-pluripotent-stem-cells-in-serum-free-3i-culture-condition-and-independent-of-lif-and-b-fgf-cytokines
#11
Yangyang Ma, Tong Yu, Yuanxing Cai, Huayan Wang
Derivation of bona fide porcine pluripotent stem cells is still a critical issue because porcine embryonic stem cells (ESCs) are not available yet, and most of the culture conditions to maintain porcine induced pluripotent stem cells (piPSCs) are based on conditions for mouse and human iPS cells. In this study, we generated a doxycycline-inducible porcine iPS cell line (DOX-iPSCs) and used it to screen the optimal culture condition to sustain the self-renewal of piPSCs. We found that LIF and b-FGF were required for porcine cell reprogramming, but were not essential cytokines for maintaining the self-renewal and pluripotency of piPSCs...
December 2018: Cell Death Discovery
https://www.readbyqxmd.com/read/29527528/reflections-on-dry-eye-syndrome-treatment-therapeutic-role-of-blood-products
#12
REVIEW
Victor J Drew, Ching-Li Tseng, Jerard Seghatchian, Thierry Burnouf
Dry eye syndrome (DES) is a multifactorial, frequent, pathology characterized by deficient tear production or increased evaporation of tears and associated with ocular surface alteration and inflammation. It mostly affects, but not exclusively, older individuals and leads to varying degrees of discomfort and decreased quality of life. Although the typical treatments of DES rely on using artificial tears, polyunsaturated fatty acids, integrin antagonists, anti-inflammatory agents, or on performing punctal occlusion, recently, standardized blood-derived serum eye drops (SED) are generating much interest as a new physiological treatment option...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29510094/flow-preservation-of-umbilical-vein-for-autologous-shunt-and-cardiovascular-reconstruction
#13
David M Hoganson, Dane A Cooper, Kimberly N Rich, Breanna L Piekarski, Liqiong Gui, Joseph P Gaut, John E Mayer, Elena Aikawa, Laura E Niklason, Sitaram M Emani
BACKGROUND: Synthetic graft materials are commonly used for shunts and cardiovascular reconstruction in neonates but are prone to thrombosis and scarring. Umbilical vein is a potential source of autologous, endothelialized tissue for neonatal shunts and tissue reconstruction but requires preservation prior to implantation. METHODS: Umbilical cords were collected in UW solution with antibiotics at 4 °C until dissection. Umbilical vein segments were tested for burst pressure before and after two weeks of preservation...
March 3, 2018: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29500387/global-phenotypic-characterisation-of-human-platelet-lysate-expanded-mscs-by-high-throughput-flow-cytometry
#14
Monica Reis, David McDonald, Lindsay Nicholson, Kathrin Godthardt, Sebastian Knobel, Anne M Dickinson, Andrew Filby, Xiao-Nong Wang
Mesenchymal stromal cells (MSCs) are a promising cell source to develop cell therapy for many diseases. Human platelet lysate (PLT) is increasingly used as an alternative to foetal calf serum (FCS) for clinical-scale MSC production. To date, the global surface protein expression of PLT-expended MSCs (MSC-PLT) is not known. To investigate this, paired MSC-PLT and MSC-FCS were analysed in parallel using high-throughput flow cytometry for the expression of 356 cell surface proteins. MSC-PLT showed differential surface protein expression compared to their MSC-FCS counterpart...
March 2, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29495420/evaluation-of-peripheral-blood-and-cord-blood-platelet-lysates-in-isolation-and-expansion-of-multipotent-mesenchymal-stromal-cells
#15
Ioanna Christou, Panagiotis Mallis, Efstathios Michalopoulos, Theofanis Chatzistamatiou, George Mermelekas, Jerome Zoidakis, Antonia Vlahou, Catherine Stavropoulos-Giokas
Abstract : Background: Multipotent Mesenchymal Stromal Cells (MSCs) are used in tissue engineering and regenerative medicine. The in vitro isolation and expansion of MSCs involve the use of foetal bovine serum (FBS). However, many concerns have been raised regarding the safety of this product. In this study, alternative additives derived either from peripheral or cord blood were tested as an FBS replacement. Methods: Platelet lysates (PL) from peripheral and cord blood were used for the expansion of MSCs. The levels of growth factors in peripheral blood (PB) and cord blood (CB) PLs were determined using the Multiple Reaction Monitoring (MRM)...
February 26, 2018: Bioengineering
https://www.readbyqxmd.com/read/29473177/manufacture-of-endothelial-colony-forming-progenitor-cells-from-steady-state-peripheral-blood-leukapheresis-using-pooled-human-platelet-lysate
#16
Georg Siegel, Erika Fleck, Stefanie Elser, Ursula Hermanutz-Klein, Marc Waidmann, Hinnak Northoff, Erhard Seifried, Richard Schäfer
BACKGROUND: Endothelial colony-forming progenitor cells (ECFCs) are promising candidates for cell therapies. However, ECFC translation to the clinic requires optimized isolation and manufacture technologies according to good manufacturing practice (GMP). STUDY DESIGN AND METHODS: ECFCs were manufactured from steady-state peripheral blood (PB) leukapheresis (11 donors), using GMP-compliant technologies including pooled human platelet (PLT) lysate, and compared to human umbilical cord endothelial cells, human aortic endothelial cells, and human cerebral microvascular endothelial cells...
February 22, 2018: Transfusion
https://www.readbyqxmd.com/read/29449599/red-blood-cells-are-dynamic-reservoirs-of-cytokines
#17
Elisabeth Karsten, Edmond Breen, Benjamin R Herbert
Red blood cells (RBCs) have been shown to affect immune function and can induce inflammatory responses after transfusion. The transfusion of washed RBCs can significantly reduce adverse effects, however, the soluble factors that may mediate these effects have not been identified. Previous studies have identified, but not quantified, a small number of chemokines associated with RBCs. We isolated RBCs from healthy volunteers and quantified of a panel of 48 cytokines, chemokines, and growth factors in the lysate, cytosol, and conditioned media of these cells using Luminex® technology...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29429942/pooled-human-serum-a-new-culture-supplement-for-bioreactor-based-cell-therapies-preliminary-results
#18
Sara Savelli, Luisa Trombi, Delfo D'Alessandro, Stefania Moscato, Simone Pacini, Simone Lapi, Fabrizio Scatena, Stefano Giannotti, Mario Petrini
BACKGROUND AIMS: Bone marrow mesenchymal stromal cells (MSCs) are an appealing source for several cell-based therapies. Many bioreactors, such as the Quantum Cell Expansion System, have been developed to generate a large number of MSCs under Good Manufacturing Practice conditions by using human platelet lysate (HPL). Previously, we isolated a novel cell population in human bone marrow, mesodermal progenitor cells (MPCs), which we identified as precursors of MSCs. MPCs may represent an important cell source for regenerative medicine applications...
February 8, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29425164/association-of-alpha-tocopherol-and-ag-sulfadiazine-chitosan-oleate-nanocarriers-in-bioactive-dressings-supporting-platelet-lysate-application-to-skin-wounds
#19
Maria Cristina Bonferoni, Giuseppina Sandri, Silvia Rossi, Eleonora Dellera, Alessandro Invernizzi, Cinzia Boselli, Antonia Icaro Cornaglia, Claudia Del Fante, Cesare Perotti, Barbara Vigani, Federica Riva, Carla Caramella, Franca Ferrari
Chitosan oleate was previously proposed to encapsulate in nanocarriers some poorly soluble molecules aimed to wound therapy, such as the anti-infective silver sulfadiazine, and the antioxidant α tocopherol. Because nanocarriers need a suitable formulation to be administered to wounds, in the present paper, these previously developed nanocarriers were loaded into freeze dried dressings based on chitosan glutamate. These were proposed as bioactive dressings aimed to support the application to wounds of platelet lysate, a hemoderivative rich in growth factors...
February 9, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29405767/assessment-of-oxidative-stress-of-platelets-among-chronic-heroin-and-hashish-addicts
#20
M A Bani-Ahmad, A G Mustafa, Aab Ahmad, Ama Rahim
INTRODUCTION: Illicit drugs abuse is associated with several clinical life-threatening consequences that are primarily mediated by oxidative damage to multiple cellular components with a subsequent cellular dysfunction and death. Primarily, this study aimed to investigate oxidative stress to protein and lipid components of circulatory platelets among chronic heroin and hashish addicts. METHODS: Platelet lysates were prepared from 20 chronic intravenously administrated heroin addicts and 20 chronic smoked hashish addicts...
January 1, 2018: Human & Experimental Toxicology
keyword
keyword
45631
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"