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Platelet lysate

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https://www.readbyqxmd.com/read/29449599/red-blood-cells-are-dynamic-reservoirs-of-cytokines
#1
Elisabeth Karsten, Edmond Breen, Benjamin R Herbert
Red blood cells (RBCs) have been shown to affect immune function and can induce inflammatory responses after transfusion. The transfusion of washed RBCs can significantly reduce adverse effects, however, the soluble factors that may mediate these effects have not been identified. Previous studies have identified, but not quantified, a small number of chemokines associated with RBCs. We isolated RBCs from healthy volunteers and quantified of a panel of 48 cytokines, chemokines, and growth factors in the lysate, cytosol, and conditioned media of these cells using Luminex ® technology...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29429942/pooled-human-serum-a-new-culture-supplement-for-bioreactor-based-cell-therapies-preliminary-results
#2
Sara Savelli, Luisa Trombi, Delfo D'Alessandro, Stefania Moscato, Simone Pacini, Simone Lapi, Fabrizio Scatena, Stefano Giannotti, Mario Petrini
BACKGROUND AIMS: Bone marrow mesenchymal stromal cells (MSCs) are an appealing source for several cell-based therapies. Many bioreactors, such as the Quantum Cell Expansion System, have been developed to generate a large number of MSCs under Good Manufacturing Practice conditions by using human platelet lysate (HPL). Previously, we isolated a novel cell population in human bone marrow, mesodermal progenitor cells (MPCs), which we identified as precursors of MSCs. MPCs may represent an important cell source for regenerative medicine applications...
February 8, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29425164/association-of-alpha-tocopherol-and-ag-sulfadiazine-chitosan-oleate-nanocarriers-in-bioactive-dressings-supporting-platelet-lysate-application-to-skin-wounds
#3
Maria Cristina Bonferoni, Giuseppina Sandri, Silvia Rossi, Eleonora Dellera, Alessandro Invernizzi, Cinzia Boselli, Antonia Icaro Cornaglia, Claudia Del Fante, Cesare Perotti, Barbara Vigani, Federica Riva, Carla Caramella, Franca Ferrari
Chitosan oleate was previously proposed to encapsulate in nanocarriers some poorly soluble molecules aimed to wound therapy, such as the anti-infective silver sulfadiazine, and the antioxidant α tocopherol. Because nanocarriers need a suitable formulation to be administered to wounds, in the present paper, these previously developed nanocarriers were loaded into freeze dried dressings based on chitosan glutamate. These were proposed as bioactive dressings aimed to support the application to wounds of platelet lysate, a hemoderivative rich in growth factors...
February 9, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29405767/assessment-of-oxidative-stress-of-platelets-among-chronic-heroin-and-hashish-addicts
#4
M A Bani-Ahmad, A G Mustafa, Aab Ahmad, Ama Rahim
INTRODUCTION: Illicit drugs abuse is associated with several clinical life-threatening consequences that are primarily mediated by oxidative damage to multiple cellular components with a subsequent cellular dysfunction and death. Primarily, this study aimed to investigate oxidative stress to protein and lipid components of circulatory platelets among chronic heroin and hashish addicts. METHODS: Platelet lysates were prepared from 20 chronic intravenously administrated heroin addicts and 20 chronic smoked hashish addicts...
January 1, 2018: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/29398623/platelet-lysate-enhances-synovial-fluid-multipotential-stromal-cells-functions-implications-for-therapeutic-use
#5
Ala Altaie, Thomas G Baboolal, Owen Wall, Elena Jones, Dennis McGonagle
BACKGROUND AIMS: Although intra-articular injection of platelet products is increasingly used for joint regenerative approaches, there are few data on their biological effects on joint-resident multipotential stromal cells (MSCs), which are directly exposed to the effects of these therapeutic strategies. Therefore, this study investigated the effect of platelet lysate (PL) on synovial fluid-derived MSCs (SF-MSCs), which in vivo have direct access to sites of cartilage injury. METHODS: SF-MSCs were obtained during knee arthroscopic procedures (N = 7)...
February 2, 2018: Cytotherapy
https://www.readbyqxmd.com/read/29383878/functional-outcome-of-human-adipose-stem-cell-injections-in-rat-anal-sphincter-acute-injury-model
#6
Kirsi Kuismanen, Miia Juntunen, Nathaniel Narra Girish, Heikki Tuominen, Heini Huhtala, Kari Nieminen, Jari Hyttinen, Susanna Miettinen
Anal incontinence is a devastating condition that significantly reduces the quality of life. Our aim was to evaluate the effect of human adipose stem cell (hASC) injections in a rat model for anal sphincter injury, which is the main cause of anal incontinence in humans. Furthermore, we tested if the efficacy of hASCs could be improved by combining them with polyacrylamide hydrogel carrier, Bulkamid®. Human ASCs derived from a female donor were culture expanded in DMEM/F12 supplemented with human platelet lysate...
January 31, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29341933/the-effects-of-platelet-lysate-patches-on-the-activity-of-tendon-derived-cells
#7
Raquel Costa-Almeida, Albina R Franco, Tamagno Pesqueira, Mariana B Oliveira, Pedro S Babo, Isabel B Leonor, João F Mano, Rui L Reis, Manuela E Gomes
Platelet-derived biomaterials are widely explored as cost-effective sources of therapeutic factors, holding a strong potential for endogenous regenerative medicine. Particularly for tendon repair, treatment approaches that shift the injury environment are explored to accelerate tendon regeneration. Herein, genipin-crosslinked platelet lysate (PL) patches are proposed for the delivery of human-derived therapeutic factors in patch augmentation strategies aiming at tendon repair. Developed PL patches exhibited a controlled release profile of PL proteins, including bFGF and PDGF-BB...
January 13, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29320421/comparative-analysis-of-different-platelet-lysates-and-platelet-rich-preparations-to-stimulate-tendon-cell-biology-an-in-vitro-study
#8
Franka Klatte-Schulz, Tanja Schmidt, Melanie Uckert, Sven Scheffler, Ulrich Kalus, Markus Rojewski, Hubert Schrezenmeier, Axel Pruss, Britt Wildemann
The poor healing potential of tendons is still a clinical problem, and the use of Platelet Rich Plasma (PRP) was hypothesized to stimulate healing. As the efficacy of PRPs remains unproven, platelet lysate (PL) could be an alternative with its main advantages of storage and characterization before use. Five different blood products were prepared from 16 male donors: human serum, two PRPs (Arthrex, (PRP-ACP); RegenLab (PRP-BCT)), platelet concentrate (apheresis, PC), and PL (freezing-thawing destruction of PC)...
January 10, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29296853/role-of-calcium-in-regulating-the-intra-and-extracellular-cleavage-of-von-willebrand-factor-by-the-protease-adamts13
#9
Shobhit Gogia, Anju Kelkar, Changjie Zhang, Kannayakanahalli M Dayananda, Sriram Neelamegham
von Willebrand factor (VWF) and the metalloprotease a disintegrin and metalloprotease with thrombospondin type 1 motif 13 (ADAMTS13) are present both within endothelial cells (ECs) and in peripheral blood. Calcium concentrations are lower in intracellular compartments (80-400 μM) compared with the extracellular milieu (∼1.25 mM). Because low calcium favors VWF A2-domain proteolysis by ADAMTS13, the dependence of proteolysis rates on calcium was assayed both within ECs and in blood. Confocal microscopy studies demonstrate partial perinuclear colocalization of VWF with ADAMTS13 in human umbilical vein ECs (HUVECs)...
October 24, 2017: Blood Advances
https://www.readbyqxmd.com/read/29289444/mineralization-by-mesenchymal-stromal-cells-is-variously-modulated-depending-on-commercial-platelet-lysate-preparations
#10
Federica Boraldi, Jorge S Burns, Angelica Bartolomeo, Massimo Dominici, Daniela Quaglino
BACKGROUND AIMS: Numerous cellular models have been developed to investigate calcification for regenerative medicine applications and for the identification of therapeutic targets in various complications associated with age-related diseases. However, results have often been contradictory due to specific culture conditions, cell type ontogeny and aging status. Human platelet lysate (hPL) has been recently investigated as valuable alternative to fetal bovine serum (FBS) in cell culture and bone regeneration...
December 27, 2017: Cytotherapy
https://www.readbyqxmd.com/read/29280928/thrombin-provokes-degranulation-of-platelet-%C3%AE-granules-leading-to-the-release-of-active-plasminogen-activator-inhibitor-1-pai-1
#11
Benjamin R Huebner, Ernest E Moore, Hunter B Moore, Gregory R Stettler, Geoffrey R Nunns, Peter Lawson, Angela Sauaia, Marguerite Kelher, Anirban Banerjee, Christopher C Silliman
BACKGROUND: The balance of fibrinolytic mediators is crucial to the survival of the critically ill patient, with tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) playing significant roles. While elevated levels of PAI-1 are associated with increased morbidity and mortality, the source of this PAI-1 remains elusive. Platelets contain 90% of circulating plasma PAI-1, however, their ability to release active PAI-1 is controversial. We hypothesize platelets contain active PAI-1 in α-granules capable of immediate degranulation when exposed to high concentrations of thrombin...
December 26, 2017: Shock
https://www.readbyqxmd.com/read/29249319/innate-immune-responses-of-equine-monocytes-cultured-in-equine-platelet-lysate
#12
Maria C Naskou, Natalie A Norton, Ian B Copland, Jacques Galipeau, John F Peroni
Platelet lysate (PL) has been extensively used for the laboratory expansion of human mesenchymal stem cells (MSC) in order to avoid fetal bovine serum (FBS) which has been associated with immune-mediated host reactions and transmission of bovine-origin microbial contaminants. Before suggesting the routine use of PL for MSC culture, we wanted to further investigate whether PL alone might trigger inflammatory responses when exposed to reactive white blood cells such as monocytes. Our objectives were to evaluate the inflammatory profile of equine monocytes cultured with equine PL (ePL) and to determine if ePL can modulate the expression of inflammatory cytokines in lipopolysaccharide (LPS)-stimulated monocytes...
January 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29214094/study-of-a-two-step-centrifugation-protocol-for-concentrating-cells-and-growth-factors-in-bovine-platelet-rich-plasma
#13
Claudia M Gutiérrez, Catalina López, Carlos E Giraldo, Jorge U Carmona
There is a lack of information about the methods used for bovine platelet-rich plasma (PRP)/platelet-rich gel (PRG) procurement, including information on platelet (PLT), white blood cell (WBC) in PRP, and growth factor release from PRG supernatants. The aims of this study were to compare and to correlate the PLT, WBC, transforming growth factor beta-1 (TGF-β1), and platelet-derived growth factor BB (PDGF-BB) concentrations in bovine whole blood, plasma, and four PRP layers and their respective PRG supernatants: A and B (obtained by a single centrifugation tube method at 720g/5 min) and C and D (obtained by a double centrifugation tube method, by using two centrifugation episodes at 720g/5 min)...
2017: Veterinary Medicine International
https://www.readbyqxmd.com/read/29209609/culture-medium-supplements-derived-from-human-platelet-and-plasma-cell-commitment-and-proliferation-support
#14
Anita Muraglia, Van Thi Nguyen, Marta Nardini, Massimo Mogni, Domenico Coviello, Beatrice Dozin, Paolo Strada, Ilaria Baldelli, Matteo Formica, Ranieri Cancedda, Maddalena Mastrogiacomo
Present cell culture medium supplements, in most cases based on animal sera, are not fully satisfactory especially for the in vitro expansion of cells intended for human cell therapy. This paper refers to (i) an heparin-free human platelet lysate (PL) devoid of serum or plasma components (v-PL) and (ii) an heparin-free human serum derived from plasma devoid of PL components (Pl-s) and to their use as single components or in combination in primary or cell line cultures. Human mesenchymal stem cells (MSC) primary cultures were obtained from adipose tissue, bone marrow, and umbilical cord...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29203267/the-significance-of-diminished-stweak-and-p-selectin-content-in-platelets-of-patients-with-pulmonary-arterial-hypertension
#15
Remigiusz Kazimierczyk, Piotr Błaszczak, Krzysztof Kowal, Małgorzata Jasiewicz, Małgorzata Knapp, Anna Szpakowicz, Katarzyna Ptaszyńska-Kopczyńska, Bożena Sobkowicz, Ewa Waszkiewicz, Ryszard Grzywna, Włodzimierz J Musial, Karol A Kamiński
Pulmonary arterial hypertension (PAH) is a progressive disease characterized by proliferative changes in pulmonary arteries. There is growing evidence suggesting that soluble tumor necrosis factor-like weak inducer of apoptosis (sTWEAK) and P-selectin could be involved in PAH development and progression. Here we investigate whether circulating platelets may be a source of sTWEAK and contribute to diminished availability of sTWEAK and P-selectin in PAH patients. We have prospectively enrolled two independent study groups of stable patients with confirmed PAH and age matched controls: derivation (10 PAH; 15 controls) and validation (20 PAH; 12 controls)...
December 1, 2017: Cytokine
https://www.readbyqxmd.com/read/29197223/does-soft-really-matter-differentiation-of-induced-pluripotent-stem-cells-into-mesenchymal-stromal-cells-is-not-influenced-by-soft-hydrogels
#16
Roman Goetzke, Julia Franzen, Alina Ostrowska, Michael Vogt, Andreas Blaeser, Gerd Klein, Björn Rath, Horst Fischer, Martin Zenke, Wolfgang Wagner
Induced pluripotent stem cells (iPSCs) can be differentiated toward mesenchymal stromal cells (MSCs), but this transition remains incomplete. It has been suggested that matrix elasticity directs cell-fate decisions. Therefore, we followed the hypothesis that differentiation of primary MSCs and generation of iPSC-derived MSCs (iMSCs) is supported by a soft matrix of human platelet lysate (hPL-gel). We demonstrate that this fibrin-based hydrogel supports growth of primary MSCs with pronounced deposition of extracellular matrix, albeit it hardly impacts on gene expression profiles or in vitro differentiation of MSCs...
November 22, 2017: Biomaterials
https://www.readbyqxmd.com/read/29180051/aptamer-based-assay-of-plated-derived-grow-factor-in-unprocessed-human-plasma-sample-and-mcf-7-breast-cancer-cell-lysates-using-gold-nanoparticle-supported-%C3%AE-cyclodextrin
#17
Mohammad Hasanzadeh, Nasrin Razmi, Ahad Mokhtarzadeh, Nasrin Shadjou, Soltanali Mahboob
Platelet-derived growth factor (PDGF), a protein biomarker, is directly involved in many cell transformation processes, such as tumor growth and progression. Elevation platelet-derived growth factor (PDGF-BB) concentration in plasma could indicate the accelerating growth of metastatic breast tumors and angiogenesis. The development of an apta-assay for detection of PDGF-BB in is presented in this work. A highly specific DNA-aptamer, selected to PDGF-BB was immobilized onto a gold nanoparticles supported α-cyclodextrin and electrochemical measurements were performed in a solution containing the phosphate buffer solution with physiological pH...
November 24, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29177632/the-use-of-lumbar-epidural-injection-of-platelet-lysate-for-treatment-of-radicular-pain
#18
Christopher Centeno, Jason Markle, Ehren Dodson, Ian Stemper, Matthew Hyzy, Christopher Williams, Michael Freeman
BACKGROUND: Epidural steroid injections (ESI) are the most common pain management procedure performed in the US, however evidence of efficacy is limited. In addition, there is early evidence that the high dose of corticosteroids used can have systemic side effects. We describe the results of a case series evaluating the use of platelet lysate (PL) epidural injections for the treatment of lumbar radicular pain as an alternative to corticosteroids. METHODS: Registry data was obtained for patients (N = 470) treated with PL epidural injections presenting with symptoms of lumbar radicular pain and MRI findings that were consistent with symptoms...
November 25, 2017: Journal of Experimental Orthopaedics
https://www.readbyqxmd.com/read/29174088/platelets-are-involved-in-in-vitro-swine-granulosa-cell-luteinization-and-angiogenesis
#19
Giuseppina Basini, Simona Bussolati, Stefano Grolli, Roberto Ramoni, Virna Conti, Fausto Quintavalla, Francesca Grasselli
During corpus luteum formation, impressive biological events take place to guarantee the transition from original follicular to luteal cells and to support required massive angiogenesis. It has been demonstrated that these phenomena resemble those essential for wound healing. After ovulation, blood vessels release their content in the antral cavity and coagulation takes place. Involvement of platelets in corpus luteum growth has been hypothesized both in human and in rat. On this basis, using platelet lysate (PL), a blood derivative with a higher platelet concentration, we aimed to assess a potential involvement of platelets in swine granulosa cell luteinization and on new blood vessel growth...
November 10, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29138638/tissue-source-and-cell-expansion-condition-influence-phenotypic-changes-of-adipose-derived-stem-cells
#20
Lauren H Mangum, Shanmugasundaram Natesan, Randolph Stone, Nicole L Wrice, David A Larson, Kyle F Florell, Barbara A Christy, Maryanne C Herzig, Andrew P Cap, Robert J Christy
Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human platelet lysate (hPL) is one potential xeno-free, alternative supplement for use in ASC culture. In this study, adipogenic and osteogenic differentiation in media supplemented with 10% FBS or 10% hPL was compared in human ASCs derived from abdominoplasty (HAP) or from adipose associated with debrided burned skin (BH)...
2017: Stem Cells International
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