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https://www.readbyqxmd.com/read/28524929/xeno-free-cultivation-of-mesenchymal-stem-cells-from-the-corneal-stroma
#1
Steffi Matthyssen, Sorcha Ní Dhubhghaill, Veerle Van Gerwen, Nadia Zakaria
Purpose: The human cornea has recently been described as a source of corneal stroma-derived mesenchymal stem cells (hMSCs). In vitro expansion of these cells involves basal medium supplemented with fetal bovine serum (FBS). As animal-derived serum can confer a risk of disease transmission and can be subject to considerable lot-to-lot variability, it does not comply with newer Good Manufacturing Practice (GMP)-required animal component-free culture protocols for clinical translation. Methods: This study investigated animal-free alternatives to FBS for cultivation of human corneal stromal MSCs...
May 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28500654/altered-timing-of-riboflavin-and-ultraviolet-light-pathogen-inactivation-improves-platelet-in-vitro-quality
#2
Peter Schubert, Brankica Culibrk, Simrath Karwal, Katherine Serrano, Elena Levin, QiLong Yi, Thomas Thiele, Andreas Greinacher, Susanne Marschner, Dana V Devine
BACKGROUND: The platelet (PLT) storage lesion is in part caused by the collection and/or production process. Pathogen inactivation (PI) further accelerates its development leading to a reduced in vitro PLT functionality and hence quality. Although the treatment of PLT concentrates (PCs) with riboflavin and ultraviolet light PI should occur within 22 hours of collection, in this study the impact of treatment timing on in vitro PLT quality was investigated. STUDY DESIGN AND METHODS: Apheresis PCs were PI treated on the day of production or on Days 1, 3, or 4 of storage or left untreated as control...
May 12, 2017: Transfusion
https://www.readbyqxmd.com/read/28470742/platelet-proteins-cause-basophil-histamine-release-through-an-immunoglobulin-dependent-mechanism
#3
Donna Dong-Young Lee, Igla Muskaj, William Savage
BACKGROUND: A general understanding of allergic transfusion reaction mechanisms remains elusive. Multiple mechanisms have been proposed, but none have been compared experimentally. STUDY DESIGN AND METHODS: We used histamine release (HR) from healthy human donor basophils to model allergic transfusion reactions. Platelet component supernatant (plasma), platelet lysate, and manipulated platelet lysates (dialyzed, delipidated, trypsinized, mild heat-inactivated, and ultracentrifuged) were used to characterize allergic stimuli...
May 4, 2017: Transfusion
https://www.readbyqxmd.com/read/28464695/mesenchymal-stem-cells-maintain-the-microenvironment-of-central-nervous-system-by-regulating-the-polarization-of-macrophages-microglia-after-traumatic-brain-injury
#4
Chao Xu, Feng Fu, Xiaohong Li, Sai Zhang
Mesenchymal Stem Cells (MSCs), which are regarded as promising candidates for cell replacement therapies, are able to regulate immune responses after traumatic brain injury (TBI). Secondary immune response following the mechanical injury is the essential factor leading to the necrosis and apoptosis of neural cells during and after the cerebral edema has subsided and there is lack of efficient agent that can mitigate such neuroinflammation in the clinical application. By means of three molecular pathways (prostaglandin E2 (PGE2), tumor necrosis factor-inducible gene 6 protein (TSG-6), and progesterone receptor (PR) and glucocorticoid receptors (GR)), MSCs induce the activation of macrophages/microglia and drive them polarize into the M2 phenotypes, which inhibits the release of pro-inflammatory cytokines and promotes tissue repair and nerve regeneration...
May 3, 2017: International Journal of Neuroscience
https://www.readbyqxmd.com/read/28460641/the-production-method-affects-the-efficacy-of-platelet-derivatives-to-expand-mesenchymal-stromal-cells-in-vitro
#5
Martina Bernardi, Francesco Agostini, Katia Chieregato, Eliana Amati, Cristina Durante, Mario Rassu, Marco Ruggeri, Sabrina Sella, Elisabetta Lombardi, Mario Mazzucato, Giuseppe Astori
BACKGROUND: The use of fetal bovine serum (FBS) as a media supplement for the ex vivo expansion of bone-marrow derived mesenchymal stromal cells (BM-MSC) has been discouraged by regulatory agencies, due to the risk of transmitting zoonoses and to elicit immune reactions in the host once transplanted. Platelet derivatives are valid FBS substitutes due to their content of growth factors that can be released disrupting the platelets by physical methods or physiological stimuli. We compared platelet derivatives produced by freezing/thawing (platelet lysates, PL) or after CaCl2 activation (platelet releasate surnatant rich in growth factors, PR-SRGF) for their content in growth factors and their ability to support the ex vivo expansion of BM-MSC...
May 1, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28453889/discrepant-platelet-and-plasma-von-willebrand-factor-in-von-willebrand-disease-patients-with-p-pro2808leufs-24
#6
Mackenzie L Bowman, Fred G Pluthero, Angie Tuttle, Lara Casey, Ling Li, Hilary Christensen, K Sue Robinson, David Lillicrap, Walter H A Kahr, Paula James
BACKGROUND: A novel variant, p.Pro2808Leufs*24, in the von Willebrand factor (VWF) gene was previously identified in the Canadian von Willebrand disease (VWD) patient population. Clinical observations of type 3 VWD patients with this variant indicate a milder bleeding phenotype compared to other type 3 patients. OBJECTIVE: To assess the effect of the Pro2808Leufs*24 variant on the molecular pathogenesis of VWD and correlate with the phenotype observed in patients...
April 28, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28439897/platelet-lysate-obtained-via-plateletpheresis-performed-in-standing-and-awake-equine-donors
#7
Scarlett M Sumner, Maria C Naskou, Merrilee Thoresen, Ian Copland, John F Peroni
BACKGROUND: Platelet preparations containing growth factors, attachment factors, and enzymes are appealing to enhance healing of injured tissues and as an alternative to xenogenic serum in cell culture media. Plateletpheresis is commonly used to collect platelets in human medicine but has not been validated in horses. STUDY DESIGN AND METHODS: Plateletpheresis to collect platelet concentrate was performed on six female, mixed breed, chemically restrained horses using commercially available apheresis equipment...
April 25, 2017: Transfusion
https://www.readbyqxmd.com/read/28375818/chondrocyte-attachment-proliferation-and-differentiation-on-three-dimensional-polycaprolactone-fumarate-scaffolds
#8
Eric R Wagner, Joshua Parry, Mahrokh Dadsetan, Dalibel Bravo, Scott M Riester, Andre J van Wijnen, Michael J Yaszemski, Sanjeev Kakar
Current treatment options for cartilage injuries are limited. The goals of this study are to create a biodegradable polymer scaffold with the capabilities of sustaining chondrocyte growth and proliferation, enable cell-to-cell communication and tissue regeneration through large pores, and assess the biological augmentation of the scaffold capabilities using platelet lysate (PL). We synthesized biodegradable polycaprolactone fumarate (PCLF) scaffolds to allow cell-cell communication through large interconnected pores...
March 31, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28368018/loss-of-the-arp2-3-complex-component-arpc1b-causes-platelet-abnormalities-and-predisposes-to-inflammatory-disease
#9
Walter H A Kahr, Fred G Pluthero, Abdul Elkadri, Neil Warner, Marko Drobac, Chang Hua Chen, Richard W Lo, Ling Li, Ren Li, Qi Li, Cornelia Thoeni, Jie Pan, Gabriella Leung, Irene Lara-Corrales, Ryan Murchie, Ernest Cutz, Ronald M Laxer, Julia Upton, Chaim M Roifman, Rae S M Yeung, John H Brumell, Aleixo M Muise
Human actin-related protein 2/3 complex (Arp2/3), required for actin filament branching, has two ARPC1 component isoforms, with ARPC1B prominently expressed in blood cells. Here we show in a child with microthrombocytopenia, eosinophilia and inflammatory disease, a homozygous frameshift mutation in ARPC1B (p.Val91Trpfs*30). Platelet lysates reveal no ARPC1B protein and greatly reduced Arp2/3 complex. Missense ARPC1B mutations are identified in an unrelated patient with similar symptoms and ARPC1B deficiency...
April 3, 2017: Nature Communications
https://www.readbyqxmd.com/read/28344760/severe-pulmonary-hypertension-in-aging-female-apolipoprotein-e-deficient-mice-is-rescued-by-estrogen-replacement-therapy
#10
Soban Umar, Rod Partow-Navid, Gregoire Ruffenach, Andrea Iorga, Shayan Moazeni, Mansoureh Eghbali
BACKGROUND: Apolipoprotein E (ApoE) is a multifunctional protein, and its deficiency leads to the development of atherosclerosis in mice. Patients with pulmonary hypertension (PH) have reduced expression of ApoE in lung tissue. ApoE is known to inhibit endothelial and smooth muscle cell proliferation and has anti-inflammatory and anti-platelet aggregation properties. Young ApoE-deficient mice have been shown to develop PH on high fat diet. The combined role of female sex and aging in the development of PH has not been investigated before...
2017: Biology of Sex Differences
https://www.readbyqxmd.com/read/28335043/platelet-derived-no-in-subjects-affected-by-type-2-diabetes-with-and-without-complications-is-there-any-relationship-with-their-offspring
#11
Jacopo Sabbatinelli, Arianna Vignini, Eleonora Salvolini, Laura Nanetti, Laura Mazzanti, Rosa Anna Rabini
Macro- and microvascular complications are currently the principal causes of morbidity and mortality in patients with diabetes mellitus. Aim of this study was to determine if type 2 diabetic patients with nephropathy and coronary artery disease showed altered platelet-derived nitric oxide (NO) production, compared with diabetic subjects without complications, and if this alteration is also present in their diabetic offspring. In this case-control observational study, platelet NO and peroxynitrite content was determined on plasma from 60 male adult type 2 diabetic patients and 60 male offspring type 2 diabetic patients...
March 23, 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/28326561/hemoxcell-a-new-oxygen-carrier-usable-as-an-additive-for-mesenchymal-stem-cell-culture-in-platelet-lysate-supplemented-media
#12
Fiona Le Pape, Lucie Cosnuau-Kemmat, Gaëlle Richard, Frédéric Dubrana, Claude Férec, Franck Zal, Elisabeth Leize, Pascal Delépine
Human mesenchymal stem cells (MSCs) are promising candidates for therapeutic applications such as tissue engineering. However, one of the main challenges is to improve oxygen supply to hypoxic areas to reduce oxygen gradient formation while preserving MSC differentiation potential and viability. For this purpose, a marine hemoglobin, HEMOXCell, was evaluated as an oxygen carrier for culturing human bone marrow MSCs in vitro for future three-dimensional culture applications. Impact of HEMOXCell on cell growth and viability was assessed in human platelet lysate (hPL)-supplemented media...
March 22, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28297568/safety-studies-for-use-of-adipose-tissue-derived-mesenchymal-stromal-stem-cells-in-a-rabbit-model-for-osteoarthritis-to-support-a-phase-i-clinical-trial
#13
Scott M Riester, Janet M Denbeigh, Yang Lin, Dakota L Jones, Tristan de Mooij, Eric A Lewallen, Hai Nie, Christopher R Paradise, Darcie J Radel, Amel Dudakovic, Emily T Camilleri, Dirk R Larson, Wenchun Qu, Aaron J Krych, Matthew A Frick, Hee-Jeong Im, Allan B Dietz, Jay Smith, Andre J van Wijnen
Adipose-derived mesenchymal stem cells (AMSCs) offer potential as a therapeutic option for clinical applications in musculoskeletal regenerative medicine because of their immunomodulatory functions and capacity for trilineage differentiation. In preparation for a phase I clinical trial using AMSCs to treat patients with osteoarthritis, we carried out preclinical studies to assess the safety of human AMSCs within the intra-articular joint space. Culture-expanded human AMSCs grown in human platelet-lysate were delivered via intra-articular injections into normal healthy rabbit knees and knees at risk for the development of osteoarthritis after bilateral medial anterior hemimeniscectomy...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28287856/enzymatic-kinetics-regarding-reversible-metabolism-of-cs-0777-a-sphingosine-1-phosphate-receptor-modulator-via-phosphorylation-and-dephosphorylation-in-humans
#14
Shin-Ichi Inaba, Maki Yamaguchi-Goto, Kaoru Tanaka-Takanaka, Kiyoaki Yonesu, Hidetaka Sakurai, Kazuishi Kubota, Takashi Izumi
1. CS-0777, a candidate compound for autoimmune diseases, becomes phosphorylated active metabolite, M1, by fructosamine 3-kinase (FN3K), FN3K-related protein (FN3K-RP); and M1 is reverted back to CS-0777 by alkaline phosphatase (ALP) in the body. We performed enzyme kinetic analysis of phosphorylation of CS-0777 by FN3K, FN3K-RP, human erythrocytes and human platelets; and dephosphorylation of M1 by various ALP isozymes and human liver, kidney, lung and small intestine microsomes. 2. The Michaelis constants of human FN3K, FN3K-RP and erythrocytes for CS-0777 phosphorylation were in the range from 498 μM to 1060 μM...
April 3, 2017: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/28269764/targeted-metabolomic-analysis-of-soluble-lysates-from-platelets-of-patients-with-mild-cognitive-impairment-and-alzheimer-s-disease-compared-to-healthy-controls-is-pc-aec40-4-a-promising-diagnostic-tool
#15
Herbert Oberacher, Kathrin Arnhard, Caroline Linhart, Angela Diwo, Josef Marksteiner, Christian Humpel
Alzheimer's disease (AD) is a progressive neurodegenerative disorder of the central nervous system. The use of biological fluids in AD diagnosis remains limited to the analysis of specific protein biomarkers in cerebrospinal fluid. However, metabolomic analysis has recently revealed several metabolites in plasma, especially phosphatidylcholines (PC), as putative biomarkers specific for AD. Following on previous reports of platelet abnormalities in AD, we hypothesized that platelets metabolites released in plasma may offer new biomarkers in AD...
2017: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/28245527/isolation-of-mesenchymal-stromal-cells-from-peripheral-blood-of-st-elevation-myocardial-infarction-patients
#16
Ina Laura Pieper, Rachel Smith, Joanna C Bishop, Omar Aldalati, Alex J Chase, Gareth Morgan, Catherine A Thornton
Bone marrow mesenchymal stromal cells (MSCs) have shown therapeutic potential in the treatment of myocardial infarction patients. However, bone marrow requires invasive harvesting techniques. Therefore, the aim was to carry out a feasibility study of using autologous peripheral blood (PB) as a source for MSCs and platelet lysate (PL), a potential novel therapeutic intervention in acute ST elevation myocardial infarction (STEMI) patients. Autologous PL and MSCs were prepared from STEMI patient and healthy control blood...
February 28, 2017: Artificial Organs
https://www.readbyqxmd.com/read/28239987/effects-of-alloantibodies-to-human-leukocyte-antigen-on-endothelial-expression-and-serum-levels-of-thrombomodulin
#17
S Béland, P Vallin, O Désy, E Lévesque, S A De Serres
Essentials The effect of alloantibodies on the endothelial expression of thrombomodulin is unknown. Thrombomodulin was quantified in stimulated endothelial cells and measured in serum samples. Anti-human leukocyte antigen (HLA) I vs. II antibodies have different effects on thrombomodulin. Anti-HLA II antibodies may promote a prothrombotic state and contribute to microangiopathy. SUMMARY: Rationale Thrombomodulin (TBM) is an anticoagulant and anti-inflammatory transmembrane protein expressed on endothelial cells...
May 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28229411/therapeutic-application-of-human-wharton-jelly-mesenchymal-stem-cells-in-skin-injury-of-scid
#18
Vikram Sabapathy, Balasubramanian Sundaram, Sanjay Kumar
Mesenchymal stem cells (MSCs) are blossoming as a credible source for regenerative medical applications. The use of fetal MSCs is gaining momentum for therapeutic use. The ease of isolation, enhanced characteristics, and immunomodulation properties renders the utilization of fetal MSCs for numerous clinical applications. In this article, we will demonstrate a step-by-step protocol for isolation of Wharton's jelly MSCs (WJMSCs) from the human umbilical cord matrix, preparation of human platelet lysate, fabricating amniotic membrane scaffold and mice model to study skin regeneration using a combination of MSCs and decellularized amniotic membrane scaffold...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28215928/human-platelet-lysate-is-a-successful-alternative-serum-supplement-for-propagation-of-monocyte-derived-dendritic-cells
#19
Urban Švajger
BACKGROUND AIMS: Clinical protocols for dendritic cell (DC) generation from monocytes require the use of animal serum-free supplements. Serum-free media can also require up to 1% of serum supplementation. In addition, recommendations based on the 3Rs (Refinement, Reduction, Replacement) principle also recommend the use of non-animal sera in in vitro studies. The aim of this study was to explore the potential use of platelet lysate (PL) for generation of optimally differentiated DCs from monocytes...
February 16, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28188071/a-good-manufacturing-practice-grade-standard-protocol-for-exclusively-human-mesenchymal-stromal-cell-derived-extracellular-vesicles
#20
Karin Pachler, Thomas Lener, Doris Streif, Zsuzsanna A Dunai, Alexandre Desgeorges, Martina Feichtner, Michaela Öller, Katharina Schallmoser, Eva Rohde, Mario Gimona
BACKGROUND AIMS: Extracellular vesicles (EVs) released by mesenchymal stromal cells (MSCs) may contribute to biological processes such as tissue regeneration, immunomodulation and neuroprotection. Evaluation of their therapeutic potential and application in future clinical trials demands thorough characterization of EV content and production under defined medium conditions, devoid of xenogenic substances and serum-derived vesicles. Addressing the apparent need for such a growth medium, we have developed a medium formulation based on pooled human platelet lysate (pHPL), free from animal-derived xenogenic additives and depleted of EVs...
February 7, 2017: Cytotherapy
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